tbc1d31 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	tbc1d31
Entrez Gene ID	448614
Full Name	TBC1 domain family member 31
Synonyms	wdr67
General protein information	Preferred Names TBC1 domain family member 31 Names TBC1 domain family member 31 WD repeat domain 67 WD repeat-containing protein 67
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018089652.1	XP_017945141.1	TBC1 domain family member 31 isoform X2
XM_018089651.2	XP_017945140.2	TBC1 domain family member 31 isoform X1
NM_001005061.1	NP_001005061.1	TBC1 domain family member 31
XM_031903315.1	XP_031759175.1	TBC1 domain family member 31 isoform X1
XM_018089650.1	XP_017945139.1	TBC1 domain family member 31 isoform X1
XM_018089651.1	XP_017945140.1	TBC1 domain family member 31 isoform X2
XM_012964537.2	XP_012819991.1	TBC1 domain family member 31 isoform X2
XM_012964537.3	XP_012819991.2	TBC1 domain family member 31 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TBC1D31	NP_663622.2
Gallus gallus (chicken)	WDR67	XP_004940076.1
Pan troglodytes (chimpanzee)	WDR67	XP_001146960.1
Bos taurus (cattle)	TBC1D31	NP_001039538.1
Xenopus tropicalis (tropical clawed frog)	tbc1d31	NP_001005061.1
Canis lupus familiaris (dog)	TBC1D31	XP_850449.1
	WDR67	XP_002805527.1
Mus musculus (house mouse)	Tbc1d31	NP_001074865.2
Rattus norvegicus (Norway rat)	Tbc1d31	NP_001128314.1
Danio rerio (zebrafish)	tbc1d31	XP_695006.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tbc1d31 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tbc1d31 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa40849	XM_018089650.1 Latest version!	Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa59621	XM_031903315.1 Latest version!	Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa59621	XM_018089651.2 Latest version!	Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa59621	XM_012964537.3 Latest version!	Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa01095	NM_001005061.1 Latest version!	Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa26723	XM_018089651.1 Latest version!	Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa26723	XM_012964537.2 Latest version!	Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa26723	XM_018089652.1 Latest version!	Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tbc1d31 ( XM_018089650.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945139.1 Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa40849
Clone ID Related Accession (Same CDS sequence)	XM_018089650.1
Accession Version	XM_018089650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TBC1 domain family member 31 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683417.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGAGT TTGAAGTTCC CGCTGTTGTG CGCGGCATGT GGGTCTCCCC AGGGGGCGGG 
GCTTGCAAAA CAGTTCGTTT CACTCACATT GCTCAGGTAC AGTACCTACC TACTGTGTTC 
TACTCACATT GCTCAGCTCA TCATCATTAC TATGCTGATA TATTAGATCT CATCTTGGAT 
CTAGGACCAA CTGCAACACT TAGCAAATCT GGCACCATGC AAACCACAGA CTTGGGCAAC 
AAAGAATCTG GAAAGATTTG GCACAGGAAG CCTAACCCAT CCACCAACGA GGGGATCATA 
GTGAATATTG TGCACGTTGA TGCATCCCAC CCCTCCAAGT CTGCACGATT TCTCCATGCT 
GGATTTGACA GCGCTGGGGA CTCCTTCATA GCAGGAGATC ACCTAGGAAA TGTCTATCTG 
TTCAACTTAA ACAGAAACAG GTTTGATCTT GTGCAGAAAA CAATGCAGGC ATGTACAGCA 
ATAGCATTCA ACCTGCATCG GAGGACAGAA TTCCTTGTGG CACTTGCTGA TAATTCAGTT 
AAGTGTTTTG ACACAGGTGC AAAGGAGCTG GTTAGTTGGA TGCGAGGCCA CGAATCTGCT 
GTCACATCCA TTTCTGTACA TCCATCTGGG AGATATGCGG TTACCACATC CAGCGATACT 
GCCCAGCTCT GGGATTTGGA TACTTTCCAA AGGAAAAGGA AACTCAATGT TCGGCAGTCT 
GTGGGAATAC AAAAGGTATT CTTTCTTCCT TTAAGTAATA CTATCTTGAG CTGCTTTAAA 
GATGACTCCA TCTTTGCCTG GGAAAGTGAT ACGCTTGCGT GCAAATACCA GTTACCTATT 
CCCGAAGATG GAACAAAGCT ACGCTATAAA GCTTTTGCCA TAACCAGGGA TGGGAGGATG 
CTGGCAGCCG GAGGAAAGTC AAACAACCTT CATCTCTGGT GTCTGGACTC CAAGCAGCTG 
TTCAGGATCA TACAGATGCC CACTAAAGTC CGAAGTGTTC AGCAGCTGGA ATTTCTGCCT 
GAAAATTTTG ATGGTGGATC CAGCCAGATT CTGGGAGTAC TAAGTCAAGA TGGAATCATG 
AGGTTTATCA ACATCCAGAC ATGCAAACTT GTGTTTGATA TCGGCAGCCA TGATAATGGA 
ATAGTGACGT CGTCTGTAAG CCCAAATGGG CGCTATATCA CAAGTGTAAT GGAGAATGGC 
AGTTTGAACA TATACAGTGT GCAAGCCCTA AGCAAAGAAC TCAACAAGCC ACCTCCACCA 
CTCGTAAAAA TGGTCGACTT ATCCAAAGAT AAAGATTCTA CTGGAAACAA GTCAGGAGTG 
AGTGGAGCAA GCCAGGAAAA GGTCAGAGTG TCCTCAGGCA GAACATGTAG GCCATGGAAG 
TCAAAGGATC AAATCGTAAG AACCAAGTAC CTGAGACCAG AAGATACCAC CACGAGTGAA 
GACAAAGAGA ATACACTACC AGCGGGATTG AGCAAACAGC GCCTACAAGC TTTGTTGAAA 
GGATTTGGGG AATACCCTGC AAAATACAGA ATGTTTATTT GGCGTTCGTT GCTCCAGCTG 
CCAGAGAACC ATGCTGCTTT CAGTAGTTTA CTTGACAAAG GCACTCATAC TCAGTACAAG 
CTTCTTCACC AGGAATATCC CATCAAAAGC AGGAAGCTCT TAAGGGTCCT TCAGAGGACA 
CTATCAGCGC TGGCTCATTG GTCGGCAATA TTCGGAGAAA CAAAATACCT GCCTCTGCTT 
GCTTTTCCCT TTGTCAAGTT GTTTCAGAAC AATCAGCTCA TTTGCTTTGA AGTGGTTGCA 
ACAGTGATTA CTAATTGGTG CCAACATTGG TTTGAATATT TTCCAAATCC CCCAATTAAC 
ATTCTTGGCA TGGTTGAGAA TCTCCTGGCG CATCACGACA AGGAACTGCT TCAGCATTTC 
ATAAATTATG GCGTGACTTC TCAGGTTTAT GCTTGGCCCC TTCTGGAAAC CCTTTTCTCT 
GAGGTTCTGA CAAGAGAGGA GTGGCTGAGG CTGTTTGACA ACGTGTTTTC CAACCATCCG 
TCATTCCTTT TGATGGCAGT CGTGTCTTAT ATCATCTCCT CCCGGTCGCC CCTGCTGCAC 
TGTAACCAAA AGGATGACTT TGAGTACTTC TTTCATCACC GGAACAATCT AGACATCGGC 
AATATGATCC GAGAAGCCTA TCATCTTATG GACACATCTC CTGCAGAAAT CCATCCTCGG 
CGCTTGCTAA GTGACTTTGA ACCTCTTACC AGAGGGCAAT ATCCCATATT TAACAAATAC 
CCCAAATTCA TTGTGGATTA TCAGGGTCAG GAACGAGAGA GGATCAGGCA GGAGGAAATA 
GAATACCTGC GAGAGAGGCA ATTGACTCAT GAAGTGGAAG CTGAGGCAGT AAAGCGCAGG 
TTGGAGGACG AGGCCTGGTA CCAGCAGCAA GAACTTCTCA AAGGGGCAGA GGAGCAGAGA 
CGGAAGCTTC TTATGGACGA GGAGCAAAAG CTTCTGCATC AGAGGCAGAG GCTGGCAACT 
GTAAAACGGG AACTCAGACT GAAAGAACTT CAGCTCCTGG ATGCAGCCAG AAGGCGGTTT 
CTGAGGTACC AGCAGGACCA GCGCAAAATG GAACTTAGAA GACTCGACGA TGAATTAGAG 
AGAAAGATGA GTTTGCGAGA ACGGGAAACT GCCACCATAG CGAAAGACGT CGAAATCAGG 
CAGATGGAGC TTGAAGCACA AAGGAGATTT TTTGAACAGC AACTTGCTAA GGAGCAGGAG 
GCTGTGACCC AGGAAGTCAA AGGAGAGATG GATGCCAACC GGAGAAGGGC CGACCTGGAA 
GAACAAATGT TTTGGAGGCT TATGGAAACA GAGGAAGACC TTAAAGATAA GAAGCTGCTT 
GAAGAGAGCC TTGCAAAGGC TGAACGTCTG TGTGTGGAAA CCGACTGGAA AATACAAACC 
CTTCAGAAAC AGAAATGTGA TGACCAGGAG CGAGGGAAGC GCTACGAAGA AGTGTCCAAA 
ATGACTGACG ATGTCCGAGA GAAAGAGCGA GAGTTATGTG ATGTTCTGAA AGCCATGGAA 
ACAAGAAAGT GGGCAGAGGT CTCTGAGAAA ATGACTCAAC TGGAAACAGA GGAGCTTGCA 
AGCTCAGCGC TGCGGGCCAA AAGGAATCAG TTCTTACAGG AGAGGTTAAG GCAGGAAGCT 
GAACCTGTTA ATATCTCAGA GGATGGAAAT GAATATTTTG AGAGACTTCG CGATTTAAGC 
CGCAATTCCA CACAGAATGA TTTCAGTAGC AGCGCTGAGC GGCACGTAGC CGAAAATGTT 
TGTTTAAACG ATGTCTCTGC AAGTGACTCC TCCACTCACT TTTCTTTAGA CAGAGGAAGA 
GGGGAGCTGG AGAACAGAGA GCGAGCCCTG ATATCAGAAG TACGAGAGCT CCGACAGAAA 
CTTGCTACCC AAGCACGCAG AAAATACCCT CAGTTGCACT TCTCTGAAAC AAACTGGACC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945139.1
CDS	70..3552
Translation	MLEFEVPAVVRGMWVSPGGGACKTVRFTHIAQVQYLPTVFYSHCSAHHHYYADILDLILDLGPTATLSKSGTMQTTDLGNKESGKIWHRKPNPSTNEGIIVNIVHVDASHPSKSARFLHAGFDSAGDSFIAGDHLGNVYLFNLNRNRFDLVQKTMQACTAIAFNLHRRTEFLVALADNSVKCFDTGAKELVSWMRGHESAVTSISVHPSGRYAVTTSSDTAQLWDLDTFQRKRKLNVRQSVGIQKVFFLPLSNTILSCFKDDSIFAWESDTLACKYQLPIPEDGTKLRYKAFAITRDGRMLAAGGKSNNLHLWCLDSKQLFRIIQMPTKVRSVQQLEFLPENFDGGSSQILGVLSQDGIMRFINIQTCKLVFDIGSHDNGIVTSSVSPNGRYITSVMENGSLNIYSVQALSKELNKPPPPLVKMVDLSKDKDSTGNKSGVSGASQEKVRVSSGRTCRPWKSKDQIVRTKYLRPEDTTTSEDKENTLPAGLSKQRLQALLKGFGEYPAKYRMFIWRSLLQLPENHAAFSSLLDKGTHTQYKLLHQEYPIKSRKLLRVLQRTLSALAHWSAIFGETKYLPLLAFPFVKLFQNNQLICFEVVATVITNWCQHWFEYFPNPPINILGMVENLLAHHDKELLQHFINYGVTSQVYAWPLLETLFSEVLTREEWLRLFDNVFSNHPSFLLMAVVSYIISSRSPLLHCNQKDDFEYFFHHRNNLDIGNMIREAYHLMDTSPAEIHPRRLLSDFEPLTRGQYPIFNKYPKFIVDYQGQERERIRQEEIEYLRERQLTHEVEAEAVKRRLEDEAWYQQQELLKGAEEQRRKLLMDEEQKLLHQRQRLATVKRELRLKELQLLDAARRRFLRYQQDQRKMELRRLDDELERKMSLRERETATIAKDVEIRQMELEAQRRFFEQQLAKEQEAVTQEVKGEMDANRRRADLEEQMFWRLMETEEDLKDKKLLEESLAKAERLCVETDWKIQTLQKQKCDDQERGKRYEEVSKMTDDVREKERELCDVLKAMETRKWAEVSEKMTQLETEELASSALRAKRNQFLQERLRQEAEPVNISEDGNEYFERLRDLSRNSTQNDFSSSAERHVAENVCLNDVSASDSSTHFSLDRGRGELENRERALISEVRELRQKLATQARRKYPQLHFSETNWT

Target ORF information:

RefSeq Version	XM_018089650.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089650.1

ORF Insert Sequence:

ATGTTGGAGT TTGAAGTTCC CGCTGTTGTG CGCGGCATGT GGGTCTCCCC AGGGGGCGGG 
GCTTGCAAAA CAGTTCGTTT CACTCACATT GCTCAGGTAC AGTACCTACC TACTGTGTTC 
TACTCACATT GCTCAGCTCA TCATCATTAC TATGCTGATA TATTAGATCT CATCTTGGAT 
CTAGGACCAA CTGCAACACT TAGCAAATCT GGCACCATGC AAACCACAGA CTTGGGCAAC 
AAAGAATCTG GAAAGATTTG GCACAGGAAG CCTAACCCAT CCACCAACGA GGGGATCATA 
GTGAATATTG TGCACGTTGA TGCATCCCAC CCCTCCAAGT CTGCACGATT TCTCCATGCT 
GGATTTGACA GCGCTGGGGA CTCCTTCATA GCAGGAGATC ACCTAGGAAA TGTCTATCTG 
TTCAACTTAA ACAGAAACAG GTTTGATCTT GTGCAGAAAA CAATGCAGGC ATGTACAGCA 
ATAGCATTCA ACCTGCATCG GAGGACAGAA TTCCTTGTGG CACTTGCTGA TAATTCAGTT 
AAGTGTTTTG ACACAGGTGC AAAGGAGCTG GTTAGTTGGA TGCGAGGCCA CGAATCTGCT 
GTCACATCCA TTTCTGTACA TCCATCTGGG AGATATGCGG TTACCACATC CAGCGATACT 
GCCCAGCTCT GGGATTTGGA TACTTTCCAA AGGAAAAGGA AACTCAATGT TCGGCAGTCT 
GTGGGAATAC AAAAGGTATT CTTTCTTCCT TTAAGTAATA CTATCTTGAG CTGCTTTAAA 
GATGACTCCA TCTTTGCCTG GGAAAGTGAT ACGCTTGCGT GCAAATACCA GTTACCTATT 
CCCGAAGATG GAACAAAGCT ACGCTATAAA GCTTTTGCCA TAACCAGGGA TGGGAGGATG 
CTGGCAGCCG GAGGAAAGTC AAACAACCTT CATCTCTGGT GTCTGGACTC CAAGCAGCTG 
TTCAGGATCA TACAGATGCC CACTAAAGTC CGAAGTGTTC AGCAGCTGGA ATTTCTGCCT 
GAAAATTTTG ATGGTGGATC CAGCCAGATT CTGGGAGTAC TAAGTCAAGA TGGAATCATG 
AGGTTTATCA ACATCCAGAC ATGCAAACTT GTGTTTGATA TCGGCAGCCA TGATAATGGA 
ATAGTGACGT CGTCTGTAAG CCCAAATGGG CGCTATATCA CAAGTGTAAT GGAGAATGGC 
AGTTTGAACA TATACAGTGT GCAAGCCCTA AGCAAAGAAC TCAACAAGCC ACCTCCACCA 
CTCGTAAAAA TGGTCGACTT ATCCAAAGAT AAAGATTCTA CTGGAAACAA GTCAGGAGTG 
AGTGGAGCAA GCCAGGAAAA GGTCAGAGTG TCCTCAGGCA GAACATGTAG GCCATGGAAG 
TCAAAGGATC AAATCGTAAG AACCAAGTAC CTGAGACCAG AAGATACCAC CACGAGTGAA 
GACAAAGAGA ATACACTACC AGCGGGATTG AGCAAACAGC GCCTACAAGC TTTGTTGAAA 
GGATTTGGGG AATACCCTGC AAAATACAGA ATGTTTATTT GGCGTTCGTT GCTCCAGCTG 
CCAGAGAACC ATGCTGCTTT CAGTAGTTTA CTTGACAAAG GCACTCATAC TCAGTACAAG 
CTTCTTCACC AGGAATATCC CATCAAAAGC AGGAAGCTCT TAAGGGTCCT TCAGAGGACA 
CTATCAGCGC TGGCTCATTG GTCGGCAATA TTCGGAGAAA CAAAATACCT GCCTCTGCTT 
GCTTTTCCCT TTGTCAAGTT GTTTCAGAAC AATCAGCTCA TTTGCTTTGA AGTGGTTGCA 
ACAGTGATTA CTAATTGGTG CCAACATTGG TTTGAATATT TTCCAAATCC CCCAATTAAC 
ATTCTTGGCA TGGTTGAGAA TCTCCTGGCG CATCACGACA AGGAACTGCT TCAGCATTTC 
ATAAATTATG GCGTGACTTC TCAGGTTTAT GCTTGGCCCC TTCTGGAAAC CCTTTTCTCT 
GAGGTTCTGA CAAGAGAGGA GTGGCTGAGG CTGTTTGACA ACGTGTTTTC CAACCATCCG 
TCATTCCTTT TGATGGCAGT CGTGTCTTAT ATCATCTCCT CCCGGTCGCC CCTGCTGCAC 
TGTAACCAAA AGGATGACTT TGAGTACTTC TTTCATCACC GGAACAATCT AGACATCGGC 
AATATGATCC GAGAAGCCTA TCATCTTATG GACACATCTC CTGCAGAAAT CCATCCTCGG 
CGCTTGCTAA GTGACTTTGA ACCTCTTACC AGAGGGCAAT ATCCCATATT TAACAAATAC 
CCCAAATTCA TTGTGGATTA TCAGGGTCAG GAACGAGAGA GGATCAGGCA GGAGGAAATA 
GAATACCTGC GAGAGAGGCA ATTGACTCAT GAAGTGGAAG CTGAGGCAGT AAAGCGCAGG 
TTGGAGGACG AGGCCTGGTA CCAGCAGCAA GAACTTCTCA AAGGGGCAGA GGAGCAGAGA 
CGGAAGCTTC TTATGGACGA GGAGCAAAAG CTTCTGCATC AGAGGCAGAG GCTGGCAACT 
GTAAAACGGG AACTCAGACT GAAAGAACTT CAGCTCCTGG ATGCAGCCAG AAGGCGGTTT 
CTGAGGTACC AGCAGGACCA GCGCAAAATG GAACTTAGAA GACTCGACGA TGAATTAGAG 
AGAAAGATGA GTTTGCGAGA ACGGGAAACT GCCACCATAG CGAAAGACGT CGAAATCAGG 
CAGATGGAGC TTGAAGCACA AAGGAGATTT TTTGAACAGC AACTTGCTAA GGAGCAGGAG 
GCTGTGACCC AGGAAGTCAA AGGAGAGATG GATGCCAACC GGAGAAGGGC CGACCTGGAA 
GAACAAATGT TTTGGAGGCT TATGGAAACA GAGGAAGACC TTAAAGATAA GAAGCTGCTT 
GAAGAGAGCC TTGCAAAGGC TGAACGTCTG TGTGTGGAAA CCGACTGGAA AATACAAACC 
CTTCAGAAAC AGAAATGTGA TGACCAGGAG CGAGGGAAGC GCTACGAAGA AGTGTCCAAA 
ATGACTGACG ATGTCCGAGA GAAAGAGCGA GAGTTATGTG ATGTTCTGAA AGCCATGGAA 
ACAAGAAAGT GGGCAGAGGT CTCTGAGAAA ATGACTCAAC TGGAAACAGA GGAGCTTGCA 
AGCTCAGCGC TGCGGGCCAA AAGGAATCAG TTCTTACAGG AGAGGTTAAG GCAGGAAGCT 
GAACCTGTTA ATATCTCAGA GGATGGAAAT GAATATTTTG AGAGACTTCG CGATTTAAGC 
CGCAATTCCA CACAGAATGA TTTCAGTAGC AGCGCTGAGC GGCACGTAGC CGAAAATGTT 
TGTTTAAACG ATGTCTCTGC AAGTGACTCC TCCACTCACT TTTCTTTAGA CAGAGGAAGA 
GGGGAGCTGG AGAACAGAGA GCGAGCCCTG ATATCAGAAG TACGAGAGCT CCGACAGAAA 
CTTGCTACCC AAGCACGCAG AAAATACCCT CAGTTGCACT TCTCTGAAAC AAACTGGACC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d31 ( XM_018089651.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945140.2 Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa59621
Clone ID Related Accession (Same CDS sequence)	XM_018089651.2 , XM_031903315.1 , XM_012964537.3
Accession Version	XM_018089651.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TBC1 domain family member 31 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018089651.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA CAGACTTGGG CAACAAAGAA TCTGGAAAGA TTTGGCACAG GAAGCCTAAC 
CCATCCACCA ACGAGGGGAT CATAGTGAAT ATTGTGCACG TTGATGCATC CCACCCCTCC 
AAGTCTGCAC GATTTCTCCA TGCTGGATTT GACAGCGCTG GGGACTCCTT CATAGCAGGA 
GATCACCTAG GAAATGTCTA TCTGTTCAAC TTAAACAGAA ACAGGTTTGA TCTTGTGCAG 
AAAACAATGC AGGCATGTAC AGCAATAGCA TTCAACCTGC ATCGGAGGAC AGAATTCCTT 
GTGGCACTTG CTGATAATTC AGTTAAGTGT TTTGACACAG GTGCAAAGGA GCTGGTTAGT 
TGGATGCGAG GCCACGAATC TGCTGTCACA TCCATTTCTG TACATCCATC TGGGAGATAT 
GCGGTTACCA CATCCAGCGA TACTGCCCAG CTCTGGGATT TGGATACTTT CCAAAGGAAA 
AGGAAACTCA ATGTTCGGCA GTCTGTGGGA ATACAAAAGG TATTCTTTCT TCCTTTAAGT 
AATACTATCT TGAGCTGCTT TAAAGATGAC TCCATCTTTG CCTGGGAAAG TGATACGCTT 
GCGTGCAAAT ACCAGTTACC TATTCCCGAA GATGGAACAA AGCTACGCTA TAAAGCTTTT 
GCCATAACCA GGGATGGGAG GATGCTGGCA GCCGGAGGAA AGTCAAACAA CCTTCATCTC 
TGGTGTCTGG ACTCCAAGCA GCTGTTCAGG ATCATACAGA TGCCCACTAA AGTCCGAAGT 
GTTCAGCAGC TGGAATTTCT GCCTGAAAAT TTTGATGGTG GATCCAGCCA GATTCTGGGA 
GTACTAAGTC AAGATGGAAT CATGAGGTTT ATCAACATCC AGACATGCAA ACTTGTGTTT 
GATATCGGCA GCCATGATAA TGGAATAGTG ACGTCGTCTG TAAGCCCAAA TGGGCGCTAT 
ATCACAAGTG TAATGGAGAA TGGCAGTTTG AACATATACA GTGTGCAAGC CCTAAGCAAA 
GAACTCAACA AGCCACCTCC ACCACTCGTA AAAATGGTCG ACTTATCCAA AGATAAAGAT 
TCTACTGGAA ACAAGTCAGG AGTGAGTGGA GCAAGCCAGG AAAAGGTCAG AGTGTCCTCA 
GGCAGAACAT GTAGGCCATG GAAGTCAAAG GATCAAATCG TAAGAACCAA GTACCTGAGA 
CCAGAAGATA CCACCACGAG TGAAGACAAA GAGAATACAC TACCAGCGGG ATTGAGCAAA 
CAGCGCCTAC AAGCTTTGTT GAAAGGATTT GGGGAATACC CTGCAAAATA CAGAATGTTT 
ATTTGGCGTT CGTTGCTCCA GCTGCCAGAG AACCATGCTG CTTTCAGTAG TTTACTTGAC 
AAAGGCACTC ATACTCAGTA CAAGCTTCTT CACCAGGAAT ATCCCATCAA AAGCAGGAAG 
CTCTTAAGGG TCCTTCAGAG GACACTATCA GCGCTGGCTC ATTGGTCGGC AATATTCGGA 
GAAACAAAAT ACCTGCCTCT GCTTGCTTTT CCCTTTGTCA AGTTGTTTCA GAACAATCAG 
CTCATTTGCT TTGAAGTGGT TGCAACAGTG ATTACTAATT GGTGCCAACA TTGGTTTGAA 
TATTTTCCAA ATCCCCCAAT TAACATTCTT GGCATGGTTG AGAATCTCCT GGCGCATCAC 
GACAAGGAAC TGCTTCAGCA TTTCATAAAT TATGGCGTGA CTTCTCAGGT TTATGCTTGG 
CCCCTTCTGG AAACCCTTTT CTCTGAGGTT CTGACAAGAG AGGAGTGGCT GAGGCTGTTT 
GACAACGTGT TTTCCAACCA TCCGTCATTC CTTTTGATGG CAGTCGTGTC TTATATCATC 
TCCTCCCGGT CGCCCCTGCT GCACTGTAAC CAAAAGGATG ACTTTGAGTA CTTCTTTCAT 
CACCGGAACA ATCTAGACAT CGGCAATATG ATCCGAGAAG CCTATCATCT TATGGACACA 
TCTCCTGCAG AAATCCATCC TCGGCGCTTG CTAAGTGACT TTGAACCTCT TACCAGAGGG 
CAATATCCCA TATTTAACAA ATACCCCAAA TTCATTGTGG ATTATCAGGG TCAGGAACGA 
GAGAGGATCA GGCAGGAGGA AATAGAATAC CTGCGAGAGA GGCAATTGAC TCATGAAGTG 
GAAGCTGAGG CAGTAAAGCG CAGGTTGGAA GATGAGGCCT GGTACCAGCA GCAAGAACTT 
CTCAAAGGGG CAGAGGAGCA GAGACGGAAG CTTCTTATGG ACGAGGAGCA AAAGCTTCTG 
CATCAGAGGC AGAGGCTGGC AACTGTAAAA CGGGAACTCA GACTGAAAGA ACTTCAGCTC 
CTGGATGCAG CCAGAAGGCG GTTTCTGAGG TACCAGCAGG ACCAGCGCAA AATGGAACTT 
AGAAGACTCG ACGATGAATT AGAGAGAAAG ATGAGTTTGC GAGAACGGGA AACTGCCACC 
ATAGCGAAAG ACGTCGAAAT CAGGCAGATG GAGCTTGAAG CACAAAGGAG ATTTTTTGAA 
CAGCAACTTG CTAAGGAGCA GGAGGCTGTG ACCCAGGAAG TCAAAGGAGA GATGGATGCC 
AACCGGAGAA GGGCCGACCT GGAAGAACAA ATGTTTTGGA GGCTTATGGA AACAGAGGAA 
GACCTTAAAG ATAAGAAGCT GCTTGAAGAG AGCCTTGCAA AGGCTGAACG TCTGTGTGTG 
GAAACCGACT GGAAAATACA AACCCTTCAG AAACAGAAAT GTGATGACCA GGAGCGAGGG 
AAGCGCTACG AAGAAGTGTC CAAAATGACT GACGATGTCC GAGAGAAAGA GCGAGAGTTA 
TGTGATGTTC TGAAAGCCAT GGAAACAAGA AAGTGGGCAG AGGTCTCTGA GAAAATGACT 
CAACTGGAAA CAGAGGAGCT TGCAAGCTCA GCGCTGCAGG CCAAAAGGAA TCGGTTCTTA 
CAGGAGAGGT TAAGGCAGGA AGCTGAACCT GTTAATATCT CAGAGGATGG AAATGAATAT 
TTTGAGAGAC TTCGCGATTT AAGCCGCAAT TCCACACAGA ATGATTTCAG TAGCAGCGCT 
GAGCGGCACG TAGCCGAAAA TGTTTGTTTA AACGATGTCT CTGCAAGCGA CTCCTCCACT 
CACTTTTCTT TAGACAGAGG AAGAGGGGAG CTGGAGAACA GAGAGCGAGC CCTGATATCA 
GAAGTACGAG AGCTCCGACA GAAACTTGCT ACCCAAGCAC GCAGAAAATA CCCTCAGTTG 
CACTTCTCTG AAACAAACTG GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945140.2
CDS	471..3737
Translation	MQTTDLGNKESGKIWHRKPNPSTNEGIIVNIVHVDASHPSKSARFLHAGFDSAGDSFIAGDHLGNVYLFNLNRNRFDLVQKTMQACTAIAFNLHRRTEFLVALADNSVKCFDTGAKELVSWMRGHESAVTSISVHPSGRYAVTTSSDTAQLWDLDTFQRKRKLNVRQSVGIQKVFFLPLSNTILSCFKDDSIFAWESDTLACKYQLPIPEDGTKLRYKAFAITRDGRMLAAGGKSNNLHLWCLDSKQLFRIIQMPTKVRSVQQLEFLPENFDGGSSQILGVLSQDGIMRFINIQTCKLVFDIGSHDNGIVTSSVSPNGRYITSVMENGSLNIYSVQALSKELNKPPPPLVKMVDLSKDKDSTGNKSGVSGASQEKVRVSSGRTCRPWKSKDQIVRTKYLRPEDTTTSEDKENTLPAGLSKQRLQALLKGFGEYPAKYRMFIWRSLLQLPENHAAFSSLLDKGTHTQYKLLHQEYPIKSRKLLRVLQRTLSALAHWSAIFGETKYLPLLAFPFVKLFQNNQLICFEVVATVITNWCQHWFEYFPNPPINILGMVENLLAHHDKELLQHFINYGVTSQVYAWPLLETLFSEVLTREEWLRLFDNVFSNHPSFLLMAVVSYIISSRSPLLHCNQKDDFEYFFHHRNNLDIGNMIREAYHLMDTSPAEIHPRRLLSDFEPLTRGQYPIFNKYPKFIVDYQGQERERIRQEEIEYLRERQLTHEVEAEAVKRRLEDEAWYQQQELLKGAEEQRRKLLMDEEQKLLHQRQRLATVKRELRLKELQLLDAARRRFLRYQQDQRKMELRRLDDELERKMSLRERETATIAKDVEIRQMELEAQRRFFEQQLAKEQEAVTQEVKGEMDANRRRADLEEQMFWRLMETEEDLKDKKLLEESLAKAERLCVETDWKIQTLQKQKCDDQERGKRYEEVSKMTDDVREKERELCDVLKAMETRKWAEVSEKMTQLETEELASSALQAKRNRFLQERLRQEAEPVNISEDGNEYFERLRDLSRNSTQNDFSSSAERHVAENVCLNDVSASDSSTHFSLDRGRGELENRERALISEVRELRQKLATQARRKYPQLHFSETNWT

Target ORF information:

RefSeq Version	XM_018089651.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089651.2

ORF Insert Sequence:

ATGCAAACCA CAGACTTGGG CAACAAAGAA TCTGGAAAGA TTTGGCACAG GAAGCCTAAC 
CCATCCACCA ACGAGGGGAT CATAGTGAAT ATTGTGCACG TTGATGCATC CCACCCCTCC 
AAGTCTGCAC GATTTCTCCA TGCTGGATTT GACAGCGCTG GGGACTCCTT CATAGCAGGA 
GATCACCTAG GAAATGTCTA TCTGTTCAAC TTAAACAGAA ACAGGTTTGA TCTTGTGCAG 
AAAACAATGC AGGCATGTAC AGCAATAGCA TTCAACCTGC ATCGGAGGAC AGAATTCCTT 
GTGGCACTTG CTGATAATTC AGTTAAGTGT TTTGACACAG GTGCAAAGGA GCTGGTTAGT 
TGGATGCGAG GCCACGAATC TGCTGTCACA TCCATTTCTG TACATCCATC TGGGAGATAT 
GCGGTTACCA CATCCAGCGA TACTGCCCAG CTCTGGGATT TGGATACTTT CCAAAGGAAA 
AGGAAACTCA ATGTTCGGCA GTCTGTGGGA ATACAAAAGG TATTCTTTCT TCCTTTAAGT 
AATACTATCT TGAGCTGCTT TAAAGATGAC TCCATCTTTG CCTGGGAAAG TGATACGCTT 
GCGTGCAAAT ACCAGTTACC TATTCCCGAA GATGGAACAA AGCTACGCTA TAAAGCTTTT 
GCCATAACCA GGGATGGGAG GATGCTGGCA GCCGGAGGAA AGTCAAACAA CCTTCATCTC 
TGGTGTCTGG ACTCCAAGCA GCTGTTCAGG ATCATACAGA TGCCCACTAA AGTCCGAAGT 
GTTCAGCAGC TGGAATTTCT GCCTGAAAAT TTTGATGGTG GATCCAGCCA GATTCTGGGA 
GTACTAAGTC AAGATGGAAT CATGAGGTTT ATCAACATCC AGACATGCAA ACTTGTGTTT 
GATATCGGCA GCCATGATAA TGGAATAGTG ACGTCGTCTG TAAGCCCAAA TGGGCGCTAT 
ATCACAAGTG TAATGGAGAA TGGCAGTTTG AACATATACA GTGTGCAAGC CCTAAGCAAA 
GAACTCAACA AGCCACCTCC ACCACTCGTA AAAATGGTCG ACTTATCCAA AGATAAAGAT 
TCTACTGGAA ACAAGTCAGG AGTGAGTGGA GCAAGCCAGG AAAAGGTCAG AGTGTCCTCA 
GGCAGAACAT GTAGGCCATG GAAGTCAAAG GATCAAATCG TAAGAACCAA GTACCTGAGA 
CCAGAAGATA CCACCACGAG TGAAGACAAA GAGAATACAC TACCAGCGGG ATTGAGCAAA 
CAGCGCCTAC AAGCTTTGTT GAAAGGATTT GGGGAATACC CTGCAAAATA CAGAATGTTT 
ATTTGGCGTT CGTTGCTCCA GCTGCCAGAG AACCATGCTG CTTTCAGTAG TTTACTTGAC 
AAAGGCACTC ATACTCAGTA CAAGCTTCTT CACCAGGAAT ATCCCATCAA AAGCAGGAAG 
CTCTTAAGGG TCCTTCAGAG GACACTATCA GCGCTGGCTC ATTGGTCGGC AATATTCGGA 
GAAACAAAAT ACCTGCCTCT GCTTGCTTTT CCCTTTGTCA AGTTGTTTCA GAACAATCAG 
CTCATTTGCT TTGAAGTGGT TGCAACAGTG ATTACTAATT GGTGCCAACA TTGGTTTGAA 
TATTTTCCAA ATCCCCCAAT TAACATTCTT GGCATGGTTG AGAATCTCCT GGCGCATCAC 
GACAAGGAAC TGCTTCAGCA TTTCATAAAT TATGGCGTGA CTTCTCAGGT TTATGCTTGG 
CCCCTTCTGG AAACCCTTTT CTCTGAGGTT CTGACAAGAG AGGAGTGGCT GAGGCTGTTT 
GACAACGTGT TTTCCAACCA TCCGTCATTC CTTTTGATGG CAGTCGTGTC TTATATCATC 
TCCTCCCGGT CGCCCCTGCT GCACTGTAAC CAAAAGGATG ACTTTGAGTA CTTCTTTCAT 
CACCGGAACA ATCTAGACAT CGGCAATATG ATCCGAGAAG CCTATCATCT TATGGACACA 
TCTCCTGCAG AAATCCATCC TCGGCGCTTG CTAAGTGACT TTGAACCTCT TACCAGAGGG 
CAATATCCCA TATTTAACAA ATACCCCAAA TTCATTGTGG ATTATCAGGG TCAGGAACGA 
GAGAGGATCA GGCAGGAGGA AATAGAATAC CTGCGAGAGA GGCAATTGAC TCATGAAGTG 
GAAGCTGAGG CAGTAAAGCG CAGGTTGGAA GATGAGGCCT GGTACCAGCA GCAAGAACTT 
CTCAAAGGGG CAGAGGAGCA GAGACGGAAG CTTCTTATGG ACGAGGAGCA AAAGCTTCTG 
CATCAGAGGC AGAGGCTGGC AACTGTAAAA CGGGAACTCA GACTGAAAGA ACTTCAGCTC 
CTGGATGCAG CCAGAAGGCG GTTTCTGAGG TACCAGCAGG ACCAGCGCAA AATGGAACTT 
AGAAGACTCG ACGATGAATT AGAGAGAAAG ATGAGTTTGC GAGAACGGGA AACTGCCACC 
ATAGCGAAAG ACGTCGAAAT CAGGCAGATG GAGCTTGAAG CACAAAGGAG ATTTTTTGAA 
CAGCAACTTG CTAAGGAGCA GGAGGCTGTG ACCCAGGAAG TCAAAGGAGA GATGGATGCC 
AACCGGAGAA GGGCCGACCT GGAAGAACAA ATGTTTTGGA GGCTTATGGA AACAGAGGAA 
GACCTTAAAG ATAAGAAGCT GCTTGAAGAG AGCCTTGCAA AGGCTGAACG TCTGTGTGTG 
GAAACCGACT GGAAAATACA AACCCTTCAG AAACAGAAAT GTGATGACCA GGAGCGAGGG 
AAGCGCTACG AAGAAGTGTC CAAAATGACT GACGATGTCC GAGAGAAAGA GCGAGAGTTA 
TGTGATGTTC TGAAAGCCAT GGAAACAAGA AAGTGGGCAG AGGTCTCTGA GAAAATGACT 
CAACTGGAAA CAGAGGAGCT TGCAAGCTCA GCGCTGCAGG CCAAAAGGAA TCGGTTCTTA 
CAGGAGAGGT TAAGGCAGGA AGCTGAACCT GTTAATATCT CAGAGGATGG AAATGAATAT 
TTTGAGAGAC TTCGCGATTT AAGCCGCAAT TCCACACAGA ATGATTTCAG TAGCAGCGCT 
GAGCGGCACG TAGCCGAAAA TGTTTGTTTA AACGATGTCT CTGCAAGCGA CTCCTCCACT 
CACTTTTCTT TAGACAGAGG AAGAGGGGAG CTGGAGAACA GAGAGCGAGC CCTGATATCA 
GAAGTACGAG AGCTCCGACA GAAACTTGCT ACCCAAGCAC GCAGAAAATA CCCTCAGTTG 
CACTTCTCTG AAACAAACTG GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d31 ( XM_031903315.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759175.1 Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X3, mRNA.

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CloneID	OXa59621
Clone ID Related Accession (Same CDS sequence)	XM_018089651.2 , XM_031903315.1 , XM_012964537.3
Accession Version	XM_031903315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TBC1 domain family member 31 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA CAGACTTGGG CAACAAAGAA TCTGGAAAGA TTTGGCACAG GAAGCCTAAC 
CCATCCACCA ACGAGGGGAT CATAGTGAAT ATTGTGCACG TTGATGCATC CCACCCCTCC 
AAGTCTGCAC GATTTCTCCA TGCTGGATTT GACAGCGCTG GGGACTCCTT CATAGCAGGA 
GATCACCTAG GAAATGTCTA TCTGTTCAAC TTAAACAGAA ACAGGTTTGA TCTTGTGCAG 
AAAACAATGC AGGCATGTAC AGCAATAGCA TTCAACCTGC ATCGGAGGAC AGAATTCCTT 
GTGGCACTTG CTGATAATTC AGTTAAGTGT TTTGACACAG GTGCAAAGGA GCTGGTTAGT 
TGGATGCGAG GCCACGAATC TGCTGTCACA TCCATTTCTG TACATCCATC TGGGAGATAT 
GCGGTTACCA CATCCAGCGA TACTGCCCAG CTCTGGGATT TGGATACTTT CCAAAGGAAA 
AGGAAACTCA ATGTTCGGCA GTCTGTGGGA ATACAAAAGG TATTCTTTCT TCCTTTAAGT 
AATACTATCT TGAGCTGCTT TAAAGATGAC TCCATCTTTG CCTGGGAAAG TGATACGCTT 
GCGTGCAAAT ACCAGTTACC TATTCCCGAA GATGGAACAA AGCTACGCTA TAAAGCTTTT 
GCCATAACCA GGGATGGGAG GATGCTGGCA GCCGGAGGAA AGTCAAACAA CCTTCATCTC 
TGGTGTCTGG ACTCCAAGCA GCTGTTCAGG ATCATACAGA TGCCCACTAA AGTCCGAAGT 
GTTCAGCAGC TGGAATTTCT GCCTGAAAAT TTTGATGGTG GATCCAGCCA GATTCTGGGA 
GTACTAAGTC AAGATGGAAT CATGAGGTTT ATCAACATCC AGACATGCAA ACTTGTGTTT 
GATATCGGCA GCCATGATAA TGGAATAGTG ACGTCGTCTG TAAGCCCAAA TGGGCGCTAT 
ATCACAAGTG TAATGGAGAA TGGCAGTTTG AACATATACA GTGTGCAAGC CCTAAGCAAA 
GAACTCAACA AGCCACCTCC ACCACTCGTA AAAATGGTCG ACTTATCCAA AGATAAAGAT 
TCTACTGGAA ACAAGTCAGG AGTGAGTGGA GCAAGCCAGG AAAAGGTCAG AGTGTCCTCA 
GGCAGAACAT GTAGGCCATG GAAGTCAAAG GATCAAATCG TAAGAACCAA GTACCTGAGA 
CCAGAAGATA CCACCACGAG TGAAGACAAA GAGAATACAC TACCAGCGGG ATTGAGCAAA 
CAGCGCCTAC AAGCTTTGTT GAAAGGATTT GGGGAATACC CTGCAAAATA CAGAATGTTT 
ATTTGGCGTT CGTTGCTCCA GCTGCCAGAG AACCATGCTG CTTTCAGTAG TTTACTTGAC 
AAAGGCACTC ATACTCAGTA CAAGCTTCTT CACCAGGAAT ATCCCATCAA AAGCAGGAAG 
CTCTTAAGGG TCCTTCAGAG GACACTATCA GCGCTGGCTC ATTGGTCGGC AATATTCGGA 
GAAACAAAAT ACCTGCCTCT GCTTGCTTTT CCCTTTGTCA AGTTGTTTCA GAACAATCAG 
CTCATTTGCT TTGAAGTGGT TGCAACAGTG ATTACTAATT GGTGCCAACA TTGGTTTGAA 
TATTTTCCAA ATCCCCCAAT TAACATTCTT GGCATGGTTG AGAATCTCCT GGCGCATCAC 
GACAAGGAAC TGCTTCAGCA TTTCATAAAT TATGGCGTGA CTTCTCAGGT TTATGCTTGG 
CCCCTTCTGG AAACCCTTTT CTCTGAGGTT CTGACAAGAG AGGAGTGGCT GAGGCTGTTT 
GACAACGTGT TTTCCAACCA TCCGTCATTC CTTTTGATGG CAGTCGTGTC TTATATCATC 
TCCTCCCGGT CGCCCCTGCT GCACTGTAAC CAAAAGGATG ACTTTGAGTA CTTCTTTCAT 
CACCGGAACA ATCTAGACAT CGGCAATATG ATCCGAGAAG CCTATCATCT TATGGACACA 
TCTCCTGCAG AAATCCATCC TCGGCGCTTG CTAAGTGACT TTGAACCTCT TACCAGAGGG 
CAATATCCCA TATTTAACAA ATACCCCAAA TTCATTGTGG ATTATCAGGG TCAGGAACGA 
GAGAGGATCA GGCAGGAGGA AATAGAATAC CTGCGAGAGA GGCAATTGAC TCATGAAGTG 
GAAGCTGAGG CAGTAAAGCG CAGGTTGGAA GATGAGGCCT GGTACCAGCA GCAAGAACTT 
CTCAAAGGGG CAGAGGAGCA GAGACGGAAG CTTCTTATGG ACGAGGAGCA AAAGCTTCTG 
CATCAGAGGC AGAGGCTGGC AACTGTAAAA CGGGAACTCA GACTGAAAGA ACTTCAGCTC 
CTGGATGCAG CCAGAAGGCG GTTTCTGAGG TACCAGCAGG ACCAGCGCAA AATGGAACTT 
AGAAGACTCG ACGATGAATT AGAGAGAAAG ATGAGTTTGC GAGAACGGGA AACTGCCACC 
ATAGCGAAAG ACGTCGAAAT CAGGCAGATG GAGCTTGAAG CACAAAGGAG ATTTTTTGAA 
CAGCAACTTG CTAAGGAGCA GGAGGCTGTG ACCCAGGAAG TCAAAGGAGA GATGGATGCC 
AACCGGAGAA GGGCCGACCT GGAAGAACAA ATGTTTTGGA GGCTTATGGA AACAGAGGAA 
GACCTTAAAG ATAAGAAGCT GCTTGAAGAG AGCCTTGCAA AGGCTGAACG TCTGTGTGTG 
GAAACCGACT GGAAAATACA AACCCTTCAG AAACAGAAAT GTGATGACCA GGAGCGAGGG 
AAGCGCTACG AAGAAGTGTC CAAAATGACT GACGATGTCC GAGAGAAAGA GCGAGAGTTA 
TGTGATGTTC TGAAAGCCAT GGAAACAAGA AAGTGGGCAG AGGTCTCTGA GAAAATGACT 
CAACTGGAAA CAGAGGAGCT TGCAAGCTCA GCGCTGCAGG CCAAAAGGAA TCGGTTCTTA 
CAGGAGAGGT TAAGGCAGGA AGCTGAACCT GTTAATATCT CAGAGGATGG AAATGAATAT 
TTTGAGAGAC TTCGCGATTT AAGCCGCAAT TCCACACAGA ATGATTTCAG TAGCAGCGCT 
GAGCGGCACG TAGCCGAAAA TGTTTGTTTA AACGATGTCT CTGCAAGCGA CTCCTCCACT 
CACTTTTCTT TAGACAGAGG AAGAGGGGAG CTGGAGAACA GAGAGCGAGC CCTGATATCA 
GAAGTACGAG AGCTCCGACA GAAACTTGCT ACCCAAGCAC GCAGAAAATA CCCTCAGTTG 
CACTTCTCTG AAACAAACTG GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759175.1
CDS	428..3694
Translation	MQTTDLGNKESGKIWHRKPNPSTNEGIIVNIVHVDASHPSKSARFLHAGFDSAGDSFIAGDHLGNVYLFNLNRNRFDLVQKTMQACTAIAFNLHRRTEFLVALADNSVKCFDTGAKELVSWMRGHESAVTSISVHPSGRYAVTTSSDTAQLWDLDTFQRKRKLNVRQSVGIQKVFFLPLSNTILSCFKDDSIFAWESDTLACKYQLPIPEDGTKLRYKAFAITRDGRMLAAGGKSNNLHLWCLDSKQLFRIIQMPTKVRSVQQLEFLPENFDGGSSQILGVLSQDGIMRFINIQTCKLVFDIGSHDNGIVTSSVSPNGRYITSVMENGSLNIYSVQALSKELNKPPPPLVKMVDLSKDKDSTGNKSGVSGASQEKVRVSSGRTCRPWKSKDQIVRTKYLRPEDTTTSEDKENTLPAGLSKQRLQALLKGFGEYPAKYRMFIWRSLLQLPENHAAFSSLLDKGTHTQYKLLHQEYPIKSRKLLRVLQRTLSALAHWSAIFGETKYLPLLAFPFVKLFQNNQLICFEVVATVITNWCQHWFEYFPNPPINILGMVENLLAHHDKELLQHFINYGVTSQVYAWPLLETLFSEVLTREEWLRLFDNVFSNHPSFLLMAVVSYIISSRSPLLHCNQKDDFEYFFHHRNNLDIGNMIREAYHLMDTSPAEIHPRRLLSDFEPLTRGQYPIFNKYPKFIVDYQGQERERIRQEEIEYLRERQLTHEVEAEAVKRRLEDEAWYQQQELLKGAEEQRRKLLMDEEQKLLHQRQRLATVKRELRLKELQLLDAARRRFLRYQQDQRKMELRRLDDELERKMSLRERETATIAKDVEIRQMELEAQRRFFEQQLAKEQEAVTQEVKGEMDANRRRADLEEQMFWRLMETEEDLKDKKLLEESLAKAERLCVETDWKIQTLQKQKCDDQERGKRYEEVSKMTDDVREKERELCDVLKAMETRKWAEVSEKMTQLETEELASSALQAKRNRFLQERLRQEAEPVNISEDGNEYFERLRDLSRNSTQNDFSSSAERHVAENVCLNDVSASDSSTHFSLDRGRGELENRERALISEVRELRQKLATQARRKYPQLHFSETNWT

Target ORF information:

RefSeq Version	XM_031903315.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903315.1

ORF Insert Sequence:

ATGCAAACCA CAGACTTGGG CAACAAAGAA TCTGGAAAGA TTTGGCACAG GAAGCCTAAC 
CCATCCACCA ACGAGGGGAT CATAGTGAAT ATTGTGCACG TTGATGCATC CCACCCCTCC 
AAGTCTGCAC GATTTCTCCA TGCTGGATTT GACAGCGCTG GGGACTCCTT CATAGCAGGA 
GATCACCTAG GAAATGTCTA TCTGTTCAAC TTAAACAGAA ACAGGTTTGA TCTTGTGCAG 
AAAACAATGC AGGCATGTAC AGCAATAGCA TTCAACCTGC ATCGGAGGAC AGAATTCCTT 
GTGGCACTTG CTGATAATTC AGTTAAGTGT TTTGACACAG GTGCAAAGGA GCTGGTTAGT 
TGGATGCGAG GCCACGAATC TGCTGTCACA TCCATTTCTG TACATCCATC TGGGAGATAT 
GCGGTTACCA CATCCAGCGA TACTGCCCAG CTCTGGGATT TGGATACTTT CCAAAGGAAA 
AGGAAACTCA ATGTTCGGCA GTCTGTGGGA ATACAAAAGG TATTCTTTCT TCCTTTAAGT 
AATACTATCT TGAGCTGCTT TAAAGATGAC TCCATCTTTG CCTGGGAAAG TGATACGCTT 
GCGTGCAAAT ACCAGTTACC TATTCCCGAA GATGGAACAA AGCTACGCTA TAAAGCTTTT 
GCCATAACCA GGGATGGGAG GATGCTGGCA GCCGGAGGAA AGTCAAACAA CCTTCATCTC 
TGGTGTCTGG ACTCCAAGCA GCTGTTCAGG ATCATACAGA TGCCCACTAA AGTCCGAAGT 
GTTCAGCAGC TGGAATTTCT GCCTGAAAAT TTTGATGGTG GATCCAGCCA GATTCTGGGA 
GTACTAAGTC AAGATGGAAT CATGAGGTTT ATCAACATCC AGACATGCAA ACTTGTGTTT 
GATATCGGCA GCCATGATAA TGGAATAGTG ACGTCGTCTG TAAGCCCAAA TGGGCGCTAT 
ATCACAAGTG TAATGGAGAA TGGCAGTTTG AACATATACA GTGTGCAAGC CCTAAGCAAA 
GAACTCAACA AGCCACCTCC ACCACTCGTA AAAATGGTCG ACTTATCCAA AGATAAAGAT 
TCTACTGGAA ACAAGTCAGG AGTGAGTGGA GCAAGCCAGG AAAAGGTCAG AGTGTCCTCA 
GGCAGAACAT GTAGGCCATG GAAGTCAAAG GATCAAATCG TAAGAACCAA GTACCTGAGA 
CCAGAAGATA CCACCACGAG TGAAGACAAA GAGAATACAC TACCAGCGGG ATTGAGCAAA 
CAGCGCCTAC AAGCTTTGTT GAAAGGATTT GGGGAATACC CTGCAAAATA CAGAATGTTT 
ATTTGGCGTT CGTTGCTCCA GCTGCCAGAG AACCATGCTG CTTTCAGTAG TTTACTTGAC 
AAAGGCACTC ATACTCAGTA CAAGCTTCTT CACCAGGAAT ATCCCATCAA AAGCAGGAAG 
CTCTTAAGGG TCCTTCAGAG GACACTATCA GCGCTGGCTC ATTGGTCGGC AATATTCGGA 
GAAACAAAAT ACCTGCCTCT GCTTGCTTTT CCCTTTGTCA AGTTGTTTCA GAACAATCAG 
CTCATTTGCT TTGAAGTGGT TGCAACAGTG ATTACTAATT GGTGCCAACA TTGGTTTGAA 
TATTTTCCAA ATCCCCCAAT TAACATTCTT GGCATGGTTG AGAATCTCCT GGCGCATCAC 
GACAAGGAAC TGCTTCAGCA TTTCATAAAT TATGGCGTGA CTTCTCAGGT TTATGCTTGG 
CCCCTTCTGG AAACCCTTTT CTCTGAGGTT CTGACAAGAG AGGAGTGGCT GAGGCTGTTT 
GACAACGTGT TTTCCAACCA TCCGTCATTC CTTTTGATGG CAGTCGTGTC TTATATCATC 
TCCTCCCGGT CGCCCCTGCT GCACTGTAAC CAAAAGGATG ACTTTGAGTA CTTCTTTCAT 
CACCGGAACA ATCTAGACAT CGGCAATATG ATCCGAGAAG CCTATCATCT TATGGACACA 
TCTCCTGCAG AAATCCATCC TCGGCGCTTG CTAAGTGACT TTGAACCTCT TACCAGAGGG 
CAATATCCCA TATTTAACAA ATACCCCAAA TTCATTGTGG ATTATCAGGG TCAGGAACGA 
GAGAGGATCA GGCAGGAGGA AATAGAATAC CTGCGAGAGA GGCAATTGAC TCATGAAGTG 
GAAGCTGAGG CAGTAAAGCG CAGGTTGGAA GATGAGGCCT GGTACCAGCA GCAAGAACTT 
CTCAAAGGGG CAGAGGAGCA GAGACGGAAG CTTCTTATGG ACGAGGAGCA AAAGCTTCTG 
CATCAGAGGC AGAGGCTGGC AACTGTAAAA CGGGAACTCA GACTGAAAGA ACTTCAGCTC 
CTGGATGCAG CCAGAAGGCG GTTTCTGAGG TACCAGCAGG ACCAGCGCAA AATGGAACTT 
AGAAGACTCG ACGATGAATT AGAGAGAAAG ATGAGTTTGC GAGAACGGGA AACTGCCACC 
ATAGCGAAAG ACGTCGAAAT CAGGCAGATG GAGCTTGAAG CACAAAGGAG ATTTTTTGAA 
CAGCAACTTG CTAAGGAGCA GGAGGCTGTG ACCCAGGAAG TCAAAGGAGA GATGGATGCC 
AACCGGAGAA GGGCCGACCT GGAAGAACAA ATGTTTTGGA GGCTTATGGA AACAGAGGAA 
GACCTTAAAG ATAAGAAGCT GCTTGAAGAG AGCCTTGCAA AGGCTGAACG TCTGTGTGTG 
GAAACCGACT GGAAAATACA AACCCTTCAG AAACAGAAAT GTGATGACCA GGAGCGAGGG 
AAGCGCTACG AAGAAGTGTC CAAAATGACT GACGATGTCC GAGAGAAAGA GCGAGAGTTA 
TGTGATGTTC TGAAAGCCAT GGAAACAAGA AAGTGGGCAG AGGTCTCTGA GAAAATGACT 
CAACTGGAAA CAGAGGAGCT TGCAAGCTCA GCGCTGCAGG CCAAAAGGAA TCGGTTCTTA 
CAGGAGAGGT TAAGGCAGGA AGCTGAACCT GTTAATATCT CAGAGGATGG AAATGAATAT 
TTTGAGAGAC TTCGCGATTT AAGCCGCAAT TCCACACAGA ATGATTTCAG TAGCAGCGCT 
GAGCGGCACG TAGCCGAAAA TGTTTGTTTA AACGATGTCT CTGCAAGCGA CTCCTCCACT 
CACTTTTCTT TAGACAGAGG AAGAGGGGAG CTGGAGAACA GAGAGCGAGC CCTGATATCA 
GAAGTACGAG AGCTCCGACA GAAACTTGCT ACCCAAGCAC GCAGAAAATA CCCTCAGTTG 
CACTTCTCTG AAACAAACTG GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d31 ( XM_012964537.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819991.2 Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X1, mRNA.

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CloneID	OXa59621
Clone ID Related Accession (Same CDS sequence)	XM_018089651.2 , XM_031903315.1 , XM_012964537.3
Accession Version	XM_012964537.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TBC1 domain family member 31 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964537.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA CAGACTTGGG CAACAAAGAA TCTGGAAAGA TTTGGCACAG GAAGCCTAAC 
CCATCCACCA ACGAGGGGAT CATAGTGAAT ATTGTGCACG TTGATGCATC CCACCCCTCC 
AAGTCTGCAC GATTTCTCCA TGCTGGATTT GACAGCGCTG GGGACTCCTT CATAGCAGGA 
GATCACCTAG GAAATGTCTA TCTGTTCAAC TTAAACAGAA ACAGGTTTGA TCTTGTGCAG 
AAAACAATGC AGGCATGTAC AGCAATAGCA TTCAACCTGC ATCGGAGGAC AGAATTCCTT 
GTGGCACTTG CTGATAATTC AGTTAAGTGT TTTGACACAG GTGCAAAGGA GCTGGTTAGT 
TGGATGCGAG GCCACGAATC TGCTGTCACA TCCATTTCTG TACATCCATC TGGGAGATAT 
GCGGTTACCA CATCCAGCGA TACTGCCCAG CTCTGGGATT TGGATACTTT CCAAAGGAAA 
AGGAAACTCA ATGTTCGGCA GTCTGTGGGA ATACAAAAGG TATTCTTTCT TCCTTTAAGT 
AATACTATCT TGAGCTGCTT TAAAGATGAC TCCATCTTTG CCTGGGAAAG TGATACGCTT 
GCGTGCAAAT ACCAGTTACC TATTCCCGAA GATGGAACAA AGCTACGCTA TAAAGCTTTT 
GCCATAACCA GGGATGGGAG GATGCTGGCA GCCGGAGGAA AGTCAAACAA CCTTCATCTC 
TGGTGTCTGG ACTCCAAGCA GCTGTTCAGG ATCATACAGA TGCCCACTAA AGTCCGAAGT 
GTTCAGCAGC TGGAATTTCT GCCTGAAAAT TTTGATGGTG GATCCAGCCA GATTCTGGGA 
GTACTAAGTC AAGATGGAAT CATGAGGTTT ATCAACATCC AGACATGCAA ACTTGTGTTT 
GATATCGGCA GCCATGATAA TGGAATAGTG ACGTCGTCTG TAAGCCCAAA TGGGCGCTAT 
ATCACAAGTG TAATGGAGAA TGGCAGTTTG AACATATACA GTGTGCAAGC CCTAAGCAAA 
GAACTCAACA AGCCACCTCC ACCACTCGTA AAAATGGTCG ACTTATCCAA AGATAAAGAT 
TCTACTGGAA ACAAGTCAGG AGTGAGTGGA GCAAGCCAGG AAAAGGTCAG AGTGTCCTCA 
GGCAGAACAT GTAGGCCATG GAAGTCAAAG GATCAAATCG TAAGAACCAA GTACCTGAGA 
CCAGAAGATA CCACCACGAG TGAAGACAAA GAGAATACAC TACCAGCGGG ATTGAGCAAA 
CAGCGCCTAC AAGCTTTGTT GAAAGGATTT GGGGAATACC CTGCAAAATA CAGAATGTTT 
ATTTGGCGTT CGTTGCTCCA GCTGCCAGAG AACCATGCTG CTTTCAGTAG TTTACTTGAC 
AAAGGCACTC ATACTCAGTA CAAGCTTCTT CACCAGGAAT ATCCCATCAA AAGCAGGAAG 
CTCTTAAGGG TCCTTCAGAG GACACTATCA GCGCTGGCTC ATTGGTCGGC AATATTCGGA 
GAAACAAAAT ACCTGCCTCT GCTTGCTTTT CCCTTTGTCA AGTTGTTTCA GAACAATCAG 
CTCATTTGCT TTGAAGTGGT TGCAACAGTG ATTACTAATT GGTGCCAACA TTGGTTTGAA 
TATTTTCCAA ATCCCCCAAT TAACATTCTT GGCATGGTTG AGAATCTCCT GGCGCATCAC 
GACAAGGAAC TGCTTCAGCA TTTCATAAAT TATGGCGTGA CTTCTCAGGT TTATGCTTGG 
CCCCTTCTGG AAACCCTTTT CTCTGAGGTT CTGACAAGAG AGGAGTGGCT GAGGCTGTTT 
GACAACGTGT TTTCCAACCA TCCGTCATTC CTTTTGATGG CAGTCGTGTC TTATATCATC 
TCCTCCCGGT CGCCCCTGCT GCACTGTAAC CAAAAGGATG ACTTTGAGTA CTTCTTTCAT 
CACCGGAACA ATCTAGACAT CGGCAATATG ATCCGAGAAG CCTATCATCT TATGGACACA 
TCTCCTGCAG AAATCCATCC TCGGCGCTTG CTAAGTGACT TTGAACCTCT TACCAGAGGG 
CAATATCCCA TATTTAACAA ATACCCCAAA TTCATTGTGG ATTATCAGGG TCAGGAACGA 
GAGAGGATCA GGCAGGAGGA AATAGAATAC CTGCGAGAGA GGCAATTGAC TCATGAAGTG 
GAAGCTGAGG CAGTAAAGCG CAGGTTGGAA GATGAGGCCT GGTACCAGCA GCAAGAACTT 
CTCAAAGGGG CAGAGGAGCA GAGACGGAAG CTTCTTATGG ACGAGGAGCA AAAGCTTCTG 
CATCAGAGGC AGAGGCTGGC AACTGTAAAA CGGGAACTCA GACTGAAAGA ACTTCAGCTC 
CTGGATGCAG CCAGAAGGCG GTTTCTGAGG TACCAGCAGG ACCAGCGCAA AATGGAACTT 
AGAAGACTCG ACGATGAATT AGAGAGAAAG ATGAGTTTGC GAGAACGGGA AACTGCCACC 
ATAGCGAAAG ACGTCGAAAT CAGGCAGATG GAGCTTGAAG CACAAAGGAG ATTTTTTGAA 
CAGCAACTTG CTAAGGAGCA GGAGGCTGTG ACCCAGGAAG TCAAAGGAGA GATGGATGCC 
AACCGGAGAA GGGCCGACCT GGAAGAACAA ATGTTTTGGA GGCTTATGGA AACAGAGGAA 
GACCTTAAAG ATAAGAAGCT GCTTGAAGAG AGCCTTGCAA AGGCTGAACG TCTGTGTGTG 
GAAACCGACT GGAAAATACA AACCCTTCAG AAACAGAAAT GTGATGACCA GGAGCGAGGG 
AAGCGCTACG AAGAAGTGTC CAAAATGACT GACGATGTCC GAGAGAAAGA GCGAGAGTTA 
TGTGATGTTC TGAAAGCCAT GGAAACAAGA AAGTGGGCAG AGGTCTCTGA GAAAATGACT 
CAACTGGAAA CAGAGGAGCT TGCAAGCTCA GCGCTGCAGG CCAAAAGGAA TCGGTTCTTA 
CAGGAGAGGT TAAGGCAGGA AGCTGAACCT GTTAATATCT CAGAGGATGG AAATGAATAT 
TTTGAGAGAC TTCGCGATTT AAGCCGCAAT TCCACACAGA ATGATTTCAG TAGCAGCGCT 
GAGCGGCACG TAGCCGAAAA TGTTTGTTTA AACGATGTCT CTGCAAGCGA CTCCTCCACT 
CACTTTTCTT TAGACAGAGG AAGAGGGGAG CTGGAGAACA GAGAGCGAGC CCTGATATCA 
GAAGTACGAG AGCTCCGACA GAAACTTGCT ACCCAAGCAC GCAGAAAATA CCCTCAGTTG 
CACTTCTCTG AAACAAACTG GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819991.2
CDS	732..3998
Translation	MQTTDLGNKESGKIWHRKPNPSTNEGIIVNIVHVDASHPSKSARFLHAGFDSAGDSFIAGDHLGNVYLFNLNRNRFDLVQKTMQACTAIAFNLHRRTEFLVALADNSVKCFDTGAKELVSWMRGHESAVTSISVHPSGRYAVTTSSDTAQLWDLDTFQRKRKLNVRQSVGIQKVFFLPLSNTILSCFKDDSIFAWESDTLACKYQLPIPEDGTKLRYKAFAITRDGRMLAAGGKSNNLHLWCLDSKQLFRIIQMPTKVRSVQQLEFLPENFDGGSSQILGVLSQDGIMRFINIQTCKLVFDIGSHDNGIVTSSVSPNGRYITSVMENGSLNIYSVQALSKELNKPPPPLVKMVDLSKDKDSTGNKSGVSGASQEKVRVSSGRTCRPWKSKDQIVRTKYLRPEDTTTSEDKENTLPAGLSKQRLQALLKGFGEYPAKYRMFIWRSLLQLPENHAAFSSLLDKGTHTQYKLLHQEYPIKSRKLLRVLQRTLSALAHWSAIFGETKYLPLLAFPFVKLFQNNQLICFEVVATVITNWCQHWFEYFPNPPINILGMVENLLAHHDKELLQHFINYGVTSQVYAWPLLETLFSEVLTREEWLRLFDNVFSNHPSFLLMAVVSYIISSRSPLLHCNQKDDFEYFFHHRNNLDIGNMIREAYHLMDTSPAEIHPRRLLSDFEPLTRGQYPIFNKYPKFIVDYQGQERERIRQEEIEYLRERQLTHEVEAEAVKRRLEDEAWYQQQELLKGAEEQRRKLLMDEEQKLLHQRQRLATVKRELRLKELQLLDAARRRFLRYQQDQRKMELRRLDDELERKMSLRERETATIAKDVEIRQMELEAQRRFFEQQLAKEQEAVTQEVKGEMDANRRRADLEEQMFWRLMETEEDLKDKKLLEESLAKAERLCVETDWKIQTLQKQKCDDQERGKRYEEVSKMTDDVREKERELCDVLKAMETRKWAEVSEKMTQLETEELASSALQAKRNRFLQERLRQEAEPVNISEDGNEYFERLRDLSRNSTQNDFSSSAERHVAENVCLNDVSASDSSTHFSLDRGRGELENRERALISEVRELRQKLATQARRKYPQLHFSETNWT

Target ORF information:

RefSeq Version	XM_012964537.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964537.3

ORF Insert Sequence:

ATGCAAACCA CAGACTTGGG CAACAAAGAA TCTGGAAAGA TTTGGCACAG GAAGCCTAAC 
CCATCCACCA ACGAGGGGAT CATAGTGAAT ATTGTGCACG TTGATGCATC CCACCCCTCC 
AAGTCTGCAC GATTTCTCCA TGCTGGATTT GACAGCGCTG GGGACTCCTT CATAGCAGGA 
GATCACCTAG GAAATGTCTA TCTGTTCAAC TTAAACAGAA ACAGGTTTGA TCTTGTGCAG 
AAAACAATGC AGGCATGTAC AGCAATAGCA TTCAACCTGC ATCGGAGGAC AGAATTCCTT 
GTGGCACTTG CTGATAATTC AGTTAAGTGT TTTGACACAG GTGCAAAGGA GCTGGTTAGT 
TGGATGCGAG GCCACGAATC TGCTGTCACA TCCATTTCTG TACATCCATC TGGGAGATAT 
GCGGTTACCA CATCCAGCGA TACTGCCCAG CTCTGGGATT TGGATACTTT CCAAAGGAAA 
AGGAAACTCA ATGTTCGGCA GTCTGTGGGA ATACAAAAGG TATTCTTTCT TCCTTTAAGT 
AATACTATCT TGAGCTGCTT TAAAGATGAC TCCATCTTTG CCTGGGAAAG TGATACGCTT 
GCGTGCAAAT ACCAGTTACC TATTCCCGAA GATGGAACAA AGCTACGCTA TAAAGCTTTT 
GCCATAACCA GGGATGGGAG GATGCTGGCA GCCGGAGGAA AGTCAAACAA CCTTCATCTC 
TGGTGTCTGG ACTCCAAGCA GCTGTTCAGG ATCATACAGA TGCCCACTAA AGTCCGAAGT 
GTTCAGCAGC TGGAATTTCT GCCTGAAAAT TTTGATGGTG GATCCAGCCA GATTCTGGGA 
GTACTAAGTC AAGATGGAAT CATGAGGTTT ATCAACATCC AGACATGCAA ACTTGTGTTT 
GATATCGGCA GCCATGATAA TGGAATAGTG ACGTCGTCTG TAAGCCCAAA TGGGCGCTAT 
ATCACAAGTG TAATGGAGAA TGGCAGTTTG AACATATACA GTGTGCAAGC CCTAAGCAAA 
GAACTCAACA AGCCACCTCC ACCACTCGTA AAAATGGTCG ACTTATCCAA AGATAAAGAT 
TCTACTGGAA ACAAGTCAGG AGTGAGTGGA GCAAGCCAGG AAAAGGTCAG AGTGTCCTCA 
GGCAGAACAT GTAGGCCATG GAAGTCAAAG GATCAAATCG TAAGAACCAA GTACCTGAGA 
CCAGAAGATA CCACCACGAG TGAAGACAAA GAGAATACAC TACCAGCGGG ATTGAGCAAA 
CAGCGCCTAC AAGCTTTGTT GAAAGGATTT GGGGAATACC CTGCAAAATA CAGAATGTTT 
ATTTGGCGTT CGTTGCTCCA GCTGCCAGAG AACCATGCTG CTTTCAGTAG TTTACTTGAC 
AAAGGCACTC ATACTCAGTA CAAGCTTCTT CACCAGGAAT ATCCCATCAA AAGCAGGAAG 
CTCTTAAGGG TCCTTCAGAG GACACTATCA GCGCTGGCTC ATTGGTCGGC AATATTCGGA 
GAAACAAAAT ACCTGCCTCT GCTTGCTTTT CCCTTTGTCA AGTTGTTTCA GAACAATCAG 
CTCATTTGCT TTGAAGTGGT TGCAACAGTG ATTACTAATT GGTGCCAACA TTGGTTTGAA 
TATTTTCCAA ATCCCCCAAT TAACATTCTT GGCATGGTTG AGAATCTCCT GGCGCATCAC 
GACAAGGAAC TGCTTCAGCA TTTCATAAAT TATGGCGTGA CTTCTCAGGT TTATGCTTGG 
CCCCTTCTGG AAACCCTTTT CTCTGAGGTT CTGACAAGAG AGGAGTGGCT GAGGCTGTTT 
GACAACGTGT TTTCCAACCA TCCGTCATTC CTTTTGATGG CAGTCGTGTC TTATATCATC 
TCCTCCCGGT CGCCCCTGCT GCACTGTAAC CAAAAGGATG ACTTTGAGTA CTTCTTTCAT 
CACCGGAACA ATCTAGACAT CGGCAATATG ATCCGAGAAG CCTATCATCT TATGGACACA 
TCTCCTGCAG AAATCCATCC TCGGCGCTTG CTAAGTGACT TTGAACCTCT TACCAGAGGG 
CAATATCCCA TATTTAACAA ATACCCCAAA TTCATTGTGG ATTATCAGGG TCAGGAACGA 
GAGAGGATCA GGCAGGAGGA AATAGAATAC CTGCGAGAGA GGCAATTGAC TCATGAAGTG 
GAAGCTGAGG CAGTAAAGCG CAGGTTGGAA GATGAGGCCT GGTACCAGCA GCAAGAACTT 
CTCAAAGGGG CAGAGGAGCA GAGACGGAAG CTTCTTATGG ACGAGGAGCA AAAGCTTCTG 
CATCAGAGGC AGAGGCTGGC AACTGTAAAA CGGGAACTCA GACTGAAAGA ACTTCAGCTC 
CTGGATGCAG CCAGAAGGCG GTTTCTGAGG TACCAGCAGG ACCAGCGCAA AATGGAACTT 
AGAAGACTCG ACGATGAATT AGAGAGAAAG ATGAGTTTGC GAGAACGGGA AACTGCCACC 
ATAGCGAAAG ACGTCGAAAT CAGGCAGATG GAGCTTGAAG CACAAAGGAG ATTTTTTGAA 
CAGCAACTTG CTAAGGAGCA GGAGGCTGTG ACCCAGGAAG TCAAAGGAGA GATGGATGCC 
AACCGGAGAA GGGCCGACCT GGAAGAACAA ATGTTTTGGA GGCTTATGGA AACAGAGGAA 
GACCTTAAAG ATAAGAAGCT GCTTGAAGAG AGCCTTGCAA AGGCTGAACG TCTGTGTGTG 
GAAACCGACT GGAAAATACA AACCCTTCAG AAACAGAAAT GTGATGACCA GGAGCGAGGG 
AAGCGCTACG AAGAAGTGTC CAAAATGACT GACGATGTCC GAGAGAAAGA GCGAGAGTTA 
TGTGATGTTC TGAAAGCCAT GGAAACAAGA AAGTGGGCAG AGGTCTCTGA GAAAATGACT 
CAACTGGAAA CAGAGGAGCT TGCAAGCTCA GCGCTGCAGG CCAAAAGGAA TCGGTTCTTA 
CAGGAGAGGT TAAGGCAGGA AGCTGAACCT GTTAATATCT CAGAGGATGG AAATGAATAT 
TTTGAGAGAC TTCGCGATTT AAGCCGCAAT TCCACACAGA ATGATTTCAG TAGCAGCGCT 
GAGCGGCACG TAGCCGAAAA TGTTTGTTTA AACGATGTCT CTGCAAGCGA CTCCTCCACT 
CACTTTTCTT TAGACAGAGG AAGAGGGGAG CTGGAGAACA GAGAGCGAGC CCTGATATCA 
GAAGTACGAG AGCTCCGACA GAAACTTGCT ACCCAAGCAC GCAGAAAATA CCCTCAGTTG 
CACTTCTCTG AAACAAACTG GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d31 ( NM_001005061.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001005061.1 Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), mRNA.

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CloneID	OXa01095
Clone ID Related Accession (Same CDS sequence)	NM_001005061.1
Accession Version	NM_001005061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TBC1 domain family member 31
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC076955.1. ##Evidence-Data-START## Transcript exon combination :: BC076955.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGCAAACCA CAGACTTGGG CAACAAAGAA TCTGGAAAGA TTTGGCACAG GAAGCCTAAC 
CCATCCACCA ACGAGGGGAT CATAGTGAAT ATTGTGCACG TTGATGCATC CCACCCCTCC 
AAGTCTGCAC GATTTCTCCA TGCTGGATTT GACAGCGCTG GGGACTCCTT CATAGCAGGA 
GATCACCTAG GAAATGTCTA TCTGTTCAAC TTAAACAGAA ACAGGTTTGA TCTTGTGCAG 
AAAACAATGC AGGCATGTAC AGCAATAGCA TTCAACCTGC ATCGGAGGAC AGAATTCCTT 
GTGGCACTTG CTGATAATTC AGTTAAGTGT TTTGACACAG GTGCAAAGGA GCTGGTTAGT 
TGGATGCGAG GCCACGAATC TGCTGTCACA TCCATTTCTG TACATCCATC TGGGAGATAT 
GCGGTTACCA CATCCAGCGA TACTGCCCAG CTCTGGGATT TGGATACTTT CCAAAGGAAA 
AGGAAACTCA ATGTTCGGCA GTCTGTGGGA ATACAAAAGG TATTCTTTCT TCCTTTAAGT 
AATACTATCT TGAGCTGCTT TAAAGATGAC TCCATCTTTG CCTGGGAAAG TGATACGCTT 
GCGTGCAAAT ACCAGTTACC TATTCCCGAA GATGGAACAA AGCTACGCTA TAAAGCTTTT 
GCCATAACCA GGGATGGGAG GATGCTGGCA GCCGGAGGAA AGTCAAACAA CCTTCATCTC 
TGGTGTCTGG ACTCCAAGCA GCTGTTCAGG ATCATACAGA TGCCCACTAA AGTCCGAAGT 
GTTCAGCAGC TGGAATTTCT GCCTGAAAAT TTTGATGGTG GATCCAGCCA GATTCTGGGA 
GTACTAAGTC AAGATGGAAT CATGAGGTTT ATCAACATCC AGACATGCAA ACTTGTGTTT 
GATATCGGCA GCCATGATAA TGGAATAGTG ACGTCGTCTG TAAGCCCAAA TGGGCGCTAT 
ATCACAAGTG TAATGGAGAA TGGCAGTTTG AACATATACA GTGTGCAAGC CCTAAGCAAA 
GAACTCAACA AGCCACCTCC ACCACTCGTA AAAATGGTCG ACTTATCCAA AGATAAAGAT 
TCTACTGGAA ACAAGTCAGG AGTGAGTGGA GCAAGCCAGG AAAAGGTCAG AGTGTCCTCA 
GGCAGAACAT GTAGGCCATG GAAGTCAAAG GATCAAATCG TAAGAACCAA GTACCTGAGA 
CCAGAAGATA CCACCACGAG TGAAGACAAA GAGAATACAC TACCAGCGGG ATTGAGCAAA 
CAGCGCCTAC AAGCTTTGTT GAAAGGATTT GGGGAATACC CTGCAAAATA CAGAATGTTT 
ATTTGGCGTT CTTTGCTCCA GCTGCCAGAG AACCATGCTG CTTTCAGTAG TTTACTTGAC 
AAAGGCACTC ATACTCAGTA CAAGCTTCTT CACCAGGAAT ATCCCATCAA AAGCAGGAAG 
CTCTTAAGGG TCCTTCAGAG GACACTATCA GCGCTGGCTC ATTGGTCGGC AATATTCGGA 
GAAACAAAAT ACCTGCCTCT GCTTGCTTTT CCCTTTGTCA AGTTGTTTCA GAACAATCAG 
CTCATTTGCT TTGAAGTGGT TGCAACAGTG ATTACTAATT GGTGCCAACA TTGGTTTGAA 
TATTTTCCAA ATCCCCCAAT TAACATTCTT GGCATGGTTG AGAATCTCCT GGCGCATCAC 
GACAAGGAAC TGCTTCAGCA TTTCATAAAT TATGGCGTGA CTTCTCAGGT TTATGCTTGG 
CCCCTTCTGG AAACCCTTTT CTCTGAGGTT CTGACAAGAG AGGAGTGGCT GAGGCTGTTT 
GACAACGTGT TTTCCAACCA TCCGTCATTC CTTTTGATGG CAGTCGTGTC TTATATCATC 
TCCTCCCGGT CGCCCCTGCT GCACTGTAAC CAAAAGGATG ACTTTGAGTA CTTCTTTCAT 
CACCGGAACA ATCTAGACAT CGGCAATATG ATCCGAGAAG CCTATCATCT TATGGACACA 
TCTCCTGCAG AAATCCATCC TCGGCGCTTG CTAAGTGACT TTGAACCTCT TACCAGAGGG 
CAATATCCCA TATTTAACAA ATACCCCAAA TTCATTGTGG ATTATCAGGG TCAGGAACGA 
GAGAGAATCA GGCAGGAGGA AATAGAATAC CTGCGAGAGA GGCAATTGAC TCATAAAGTG 
GAAGCTGAGG CAGTAAAGCG CAGGTTGGAG GACGAGGCCT GGTACCAGCA GCAAGAACTT 
CTCAAAGGGG CAGAGGAGCA GAGACGGAAG CTTCTTATGG ACGAGGAGCA AAAGCTTCTG 
CATCAGAGGC AGAGGCTGGC AACTGTAAAA CGGGAACTCA GACTGAAAGA ACTTCAGCTC 
CTGGATGCAG CCAGAAGGCG GTTTCTGAGG TACCAGCAGG ACCAGCGCAA AATGGAACTT 
AGAAGACTCG ACGATGAATT AGAGAGAAAG ATGAGTTTGC GAGAACGGGA AACTGCCACC 
ATAGCGAAAG ACGTTGAAAT CAGGCAGATG GAGCTTGAAG CACAAAGGAG ATTTTTTGAA 
CAGCAACTTG CTAAGGAGCA GGAGGCTGTG ACCCAGGAAG TCAAAGGAGA GATGGATGCC 
AACCGGAGAA GGGCCGACCT GGAAGAACAA ATGTTTTGGA GGCTTATGGA AACAGAGGAA 
GACCTTAAAG ATAAGAAGCT GCTTGAAGAG AGCCTTGCAA AGGCTGAACG TCTGTGTGTG 
GAAACCGACT GGAAAATACA AACCCTTCAG AAACAGAAAT GTGATGACCA GGAGCGAGGG 
AAGCGCTACG TAGAAGTGTC CAAAATGACT GACGATGTCC GAGAGAAAGA GCGAGAGTTA 
TGTGATGTTC TGAAAGCCAT GGAAACAAGA AAGTGGGCAG AGGTCTCTGA GAAAATGACT 
CAACTGGAAA CAGAGGAGCT TGCAAGCTCA GCGCTGCGGG CCAAAAGGAA TCAGTTCTTA 
CAGGAGAGGT TAAGGCAGGA AGCTGAACCT GTTAATATCT CAGAGGATGG AAATGAATAT 
TTTGAGAGAC TTCGCGATTT AAGCCGCAAT TCCACACAGA ATGATTTCAG TAGCAGCGCT 
GAGCGGCACG TAGCCGAAAA TGTTTGTTTA AACGATGTCT CTGCAAGTGA CTCCTCCACT 
CACTTTTCTT TAGACAGAGG AAGAGGGGAG CTGGAGAACA GAGAGCGAGC CCTGATATCA 
GAAGTACGAG AGCTCCGACA GAAACTTGCT ACCCAAGCAC GCAGAAAATA CCCTCAGTTG 
CACTTCTCTG AAACAAACTG GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005061.1
CDS	242..3508
Translation	MQTTDLGNKESGKIWHRKPNPSTNEGIIVNIVHVDASHPSKSARFLHAGFDSAGDSFIAGDHLGNVYLFNLNRNRFDLVQKTMQACTAIAFNLHRRTEFLVALADNSVKCFDTGAKELVSWMRGHESAVTSISVHPSGRYAVTTSSDTAQLWDLDTFQRKRKLNVRQSVGIQKVFFLPLSNTILSCFKDDSIFAWESDTLACKYQLPIPEDGTKLRYKAFAITRDGRMLAAGGKSNNLHLWCLDSKQLFRIIQMPTKVRSVQQLEFLPENFDGGSSQILGVLSQDGIMRFINIQTCKLVFDIGSHDNGIVTSSVSPNGRYITSVMENGSLNIYSVQALSKELNKPPPPLVKMVDLSKDKDSTGNKSGVSGASQEKVRVSSGRTCRPWKSKDQIVRTKYLRPEDTTTSEDKENTLPAGLSKQRLQALLKGFGEYPAKYRMFIWRSLLQLPENHAAFSSLLDKGTHTQYKLLHQEYPIKSRKLLRVLQRTLSALAHWSAIFGETKYLPLLAFPFVKLFQNNQLICFEVVATVITNWCQHWFEYFPNPPINILGMVENLLAHHDKELLQHFINYGVTSQVYAWPLLETLFSEVLTREEWLRLFDNVFSNHPSFLLMAVVSYIISSRSPLLHCNQKDDFEYFFHHRNNLDIGNMIREAYHLMDTSPAEIHPRRLLSDFEPLTRGQYPIFNKYPKFIVDYQGQERERIRQEEIEYLRERQLTHKVEAEAVKRRLEDEAWYQQQELLKGAEEQRRKLLMDEEQKLLHQRQRLATVKRELRLKELQLLDAARRRFLRYQQDQRKMELRRLDDELERKMSLRERETATIAKDVEIRQMELEAQRRFFEQQLAKEQEAVTQEVKGEMDANRRRADLEEQMFWRLMETEEDLKDKKLLEESLAKAERLCVETDWKIQTLQKQKCDDQERGKRYVEVSKMTDDVREKERELCDVLKAMETRKWAEVSEKMTQLETEELASSALRAKRNQFLQERLRQEAEPVNISEDGNEYFERLRDLSRNSTQNDFSSSAERHVAENVCLNDVSASDSSTHFSLDRGRGELENRERALISEVRELRQKLATQARRKYPQLHFSETNWT

Target ORF information:

RefSeq Version	NM_001005061.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005061.1

ORF Insert Sequence:

ATGCAAACCA CAGACTTGGG CAACAAAGAA TCTGGAAAGA TTTGGCACAG GAAGCCTAAC 
CCATCCACCA ACGAGGGGAT CATAGTGAAT ATTGTGCACG TTGATGCATC CCACCCCTCC 
AAGTCTGCAC GATTTCTCCA TGCTGGATTT GACAGCGCTG GGGACTCCTT CATAGCAGGA 
GATCACCTAG GAAATGTCTA TCTGTTCAAC TTAAACAGAA ACAGGTTTGA TCTTGTGCAG 
AAAACAATGC AGGCATGTAC AGCAATAGCA TTCAACCTGC ATCGGAGGAC AGAATTCCTT 
GTGGCACTTG CTGATAATTC AGTTAAGTGT TTTGACACAG GTGCAAAGGA GCTGGTTAGT 
TGGATGCGAG GCCACGAATC TGCTGTCACA TCCATTTCTG TACATCCATC TGGGAGATAT 
GCGGTTACCA CATCCAGCGA TACTGCCCAG CTCTGGGATT TGGATACTTT CCAAAGGAAA 
AGGAAACTCA ATGTTCGGCA GTCTGTGGGA ATACAAAAGG TATTCTTTCT TCCTTTAAGT 
AATACTATCT TGAGCTGCTT TAAAGATGAC TCCATCTTTG CCTGGGAAAG TGATACGCTT 
GCGTGCAAAT ACCAGTTACC TATTCCCGAA GATGGAACAA AGCTACGCTA TAAAGCTTTT 
GCCATAACCA GGGATGGGAG GATGCTGGCA GCCGGAGGAA AGTCAAACAA CCTTCATCTC 
TGGTGTCTGG ACTCCAAGCA GCTGTTCAGG ATCATACAGA TGCCCACTAA AGTCCGAAGT 
GTTCAGCAGC TGGAATTTCT GCCTGAAAAT TTTGATGGTG GATCCAGCCA GATTCTGGGA 
GTACTAAGTC AAGATGGAAT CATGAGGTTT ATCAACATCC AGACATGCAA ACTTGTGTTT 
GATATCGGCA GCCATGATAA TGGAATAGTG ACGTCGTCTG TAAGCCCAAA TGGGCGCTAT 
ATCACAAGTG TAATGGAGAA TGGCAGTTTG AACATATACA GTGTGCAAGC CCTAAGCAAA 
GAACTCAACA AGCCACCTCC ACCACTCGTA AAAATGGTCG ACTTATCCAA AGATAAAGAT 
TCTACTGGAA ACAAGTCAGG AGTGAGTGGA GCAAGCCAGG AAAAGGTCAG AGTGTCCTCA 
GGCAGAACAT GTAGGCCATG GAAGTCAAAG GATCAAATCG TAAGAACCAA GTACCTGAGA 
CCAGAAGATA CCACCACGAG TGAAGACAAA GAGAATACAC TACCAGCGGG ATTGAGCAAA 
CAGCGCCTAC AAGCTTTGTT GAAAGGATTT GGGGAATACC CTGCAAAATA CAGAATGTTT 
ATTTGGCGTT CTTTGCTCCA GCTGCCAGAG AACCATGCTG CTTTCAGTAG TTTACTTGAC 
AAAGGCACTC ATACTCAGTA CAAGCTTCTT CACCAGGAAT ATCCCATCAA AAGCAGGAAG 
CTCTTAAGGG TCCTTCAGAG GACACTATCA GCGCTGGCTC ATTGGTCGGC AATATTCGGA 
GAAACAAAAT ACCTGCCTCT GCTTGCTTTT CCCTTTGTCA AGTTGTTTCA GAACAATCAG 
CTCATTTGCT TTGAAGTGGT TGCAACAGTG ATTACTAATT GGTGCCAACA TTGGTTTGAA 
TATTTTCCAA ATCCCCCAAT TAACATTCTT GGCATGGTTG AGAATCTCCT GGCGCATCAC 
GACAAGGAAC TGCTTCAGCA TTTCATAAAT TATGGCGTGA CTTCTCAGGT TTATGCTTGG 
CCCCTTCTGG AAACCCTTTT CTCTGAGGTT CTGACAAGAG AGGAGTGGCT GAGGCTGTTT 
GACAACGTGT TTTCCAACCA TCCGTCATTC CTTTTGATGG CAGTCGTGTC TTATATCATC 
TCCTCCCGGT CGCCCCTGCT GCACTGTAAC CAAAAGGATG ACTTTGAGTA CTTCTTTCAT 
CACCGGAACA ATCTAGACAT CGGCAATATG ATCCGAGAAG CCTATCATCT TATGGACACA 
TCTCCTGCAG AAATCCATCC TCGGCGCTTG CTAAGTGACT TTGAACCTCT TACCAGAGGG 
CAATATCCCA TATTTAACAA ATACCCCAAA TTCATTGTGG ATTATCAGGG TCAGGAACGA 
GAGAGAATCA GGCAGGAGGA AATAGAATAC CTGCGAGAGA GGCAATTGAC TCATAAAGTG 
GAAGCTGAGG CAGTAAAGCG CAGGTTGGAG GACGAGGCCT GGTACCAGCA GCAAGAACTT 
CTCAAAGGGG CAGAGGAGCA GAGACGGAAG CTTCTTATGG ACGAGGAGCA AAAGCTTCTG 
CATCAGAGGC AGAGGCTGGC AACTGTAAAA CGGGAACTCA GACTGAAAGA ACTTCAGCTC 
CTGGATGCAG CCAGAAGGCG GTTTCTGAGG TACCAGCAGG ACCAGCGCAA AATGGAACTT 
AGAAGACTCG ACGATGAATT AGAGAGAAAG ATGAGTTTGC GAGAACGGGA AACTGCCACC 
ATAGCGAAAG ACGTTGAAAT CAGGCAGATG GAGCTTGAAG CACAAAGGAG ATTTTTTGAA 
CAGCAACTTG CTAAGGAGCA GGAGGCTGTG ACCCAGGAAG TCAAAGGAGA GATGGATGCC 
AACCGGAGAA GGGCCGACCT GGAAGAACAA ATGTTTTGGA GGCTTATGGA AACAGAGGAA 
GACCTTAAAG ATAAGAAGCT GCTTGAAGAG AGCCTTGCAA AGGCTGAACG TCTGTGTGTG 
GAAACCGACT GGAAAATACA AACCCTTCAG AAACAGAAAT GTGATGACCA GGAGCGAGGG 
AAGCGCTACG TAGAAGTGTC CAAAATGACT GACGATGTCC GAGAGAAAGA GCGAGAGTTA 
TGTGATGTTC TGAAAGCCAT GGAAACAAGA AAGTGGGCAG AGGTCTCTGA GAAAATGACT 
CAACTGGAAA CAGAGGAGCT TGCAAGCTCA GCGCTGCGGG CCAAAAGGAA TCAGTTCTTA 
CAGGAGAGGT TAAGGCAGGA AGCTGAACCT GTTAATATCT CAGAGGATGG AAATGAATAT 
TTTGAGAGAC TTCGCGATTT AAGCCGCAAT TCCACACAGA ATGATTTCAG TAGCAGCGCT 
GAGCGGCACG TAGCCGAAAA TGTTTGTTTA AACGATGTCT CTGCAAGTGA CTCCTCCACT 
CACTTTTCTT TAGACAGAGG AAGAGGGGAG CTGGAGAACA GAGAGCGAGC CCTGATATCA 
GAAGTACGAG AGCTCCGACA GAAACTTGCT ACCCAAGCAC GCAGAAAATA CCCTCAGTTG 
CACTTCTCTG AAACAAACTG GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d31 ( XM_018089652.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945141.1 Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa26723
Clone ID Related Accession (Same CDS sequence)	XM_018089652.1 , XM_018089651.1 , XM_012964537.2
Accession Version	XM_018089652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TBC1 domain family member 31 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683417.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA CAGACTTGGG CAACAAAGAA TCTGGAAAGA TTTGGCACAG GAAGCCTAAC 
CCATCCACCA ACGAGGGGAT CATAGTGAAT ATTGTGCACG TTGATGCATC CCACCCCTCC 
AAGTCTGCAC GATTTCTCCA TGCTGGATTT GACAGCGCTG GGGACTCCTT CATAGCAGGA 
GATCACCTAG GAAATGTCTA TCTGTTCAAC TTAAACAGAA ACAGGTTTGA TCTTGTGCAG 
AAAACAATGC AGGCATGTAC AGCAATAGCA TTCAACCTGC ATCGGAGGAC AGAATTCCTT 
GTGGCACTTG CTGATAATTC AGTTAAGTGT TTTGACACAG GTGCAAAGGA GCTGGTTAGT 
TGGATGCGAG GCCACGAATC TGCTGTCACA TCCATTTCTG TACATCCATC TGGGAGATAT 
GCGGTTACCA CATCCAGCGA TACTGCCCAG CTCTGGGATT TGGATACTTT CCAAAGGAAA 
AGGAAACTCA ATGTTCGGCA GTCTGTGGGA ATACAAAAGG TATTCTTTCT TCCTTTAAGT 
AATACTATCT TGAGCTGCTT TAAAGATGAC TCCATCTTTG CCTGGGAAAG TGATACGCTT 
GCGTGCAAAT ACCAGTTACC TATTCCCGAA GATGGAACAA AGCTACGCTA TAAAGCTTTT 
GCCATAACCA GGGATGGGAG GATGCTGGCA GCCGGAGGAA AGTCAAACAA CCTTCATCTC 
TGGTGTCTGG ACTCCAAGCA GCTGTTCAGG ATCATACAGA TGCCCACTAA AGTCCGAAGT 
GTTCAGCAGC TGGAATTTCT GCCTGAAAAT TTTGATGGTG GATCCAGCCA GATTCTGGGA 
GTACTAAGTC AAGATGGAAT CATGAGGTTT ATCAACATCC AGACATGCAA ACTTGTGTTT 
GATATCGGCA GCCATGATAA TGGAATAGTG ACGTCGTCTG TAAGCCCAAA TGGGCGCTAT 
ATCACAAGTG TAATGGAGAA TGGCAGTTTG AACATATACA GTGTGCAAGC CCTAAGCAAA 
GAACTCAACA AGCCACCTCC ACCACTCGTA AAAATGGTCG ACTTATCCAA AGATAAAGAT 
TCTACTGGAA ACAAGTCAGG AGTGAGTGGA GCAAGCCAGG AAAAGGTCAG AGTGTCCTCA 
GGCAGAACAT GTAGGCCATG GAAGTCAAAG GATCAAATCG TAAGAACCAA GTACCTGAGA 
CCAGAAGATA CCACCACGAG TGAAGACAAA GAGAATACAC TACCAGCGGG ATTGAGCAAA 
CAGCGCCTAC AAGCTTTGTT GAAAGGATTT GGGGAATACC CTGCAAAATA CAGAATGTTT 
ATTTGGCGTT CGTTGCTCCA GCTGCCAGAG AACCATGCTG CTTTCAGTAG TTTACTTGAC 
AAAGGCACTC ATACTCAGTA CAAGCTTCTT CACCAGGAAT ATCCCATCAA AAGCAGGAAG 
CTCTTAAGGG TCCTTCAGAG GACACTATCA GCGCTGGCTC ATTGGTCGGC AATATTCGGA 
GAAACAAAAT ACCTGCCTCT GCTTGCTTTT CCCTTTGTCA AGTTGTTTCA GAACAATCAG 
CTCATTTGCT TTGAAGTGGT TGCAACAGTG ATTACTAATT GGTGCCAACA TTGGTTTGAA 
TATTTTCCAA ATCCCCCAAT TAACATTCTT GGCATGGTTG AGAATCTCCT GGCGCATCAC 
GACAAGGAAC TGCTTCAGCA TTTCATAAAT TATGGCGTGA CTTCTCAGGT TTATGCTTGG 
CCCCTTCTGG AAACCCTTTT CTCTGAGGTT CTGACAAGAG AGGAGTGGCT GAGGCTGTTT 
GACAACGTGT TTTCCAACCA TCCGTCATTC CTTTTGATGG CAGTCGTGTC TTATATCATC 
TCCTCCCGGT CGCCCCTGCT GCACTGTAAC CAAAAGGATG ACTTTGAGTA CTTCTTTCAT 
CACCGGAACA ATCTAGACAT CGGCAATATG ATCCGAGAAG CCTATCATCT TATGGACACA 
TCTCCTGCAG AAATCCATCC TCGGCGCTTG CTAAGTGACT TTGAACCTCT TACCAGAGGG 
CAATATCCCA TATTTAACAA ATACCCCAAA TTCATTGTGG ATTATCAGGG TCAGGAACGA 
GAGAGGATCA GGCAGGAGGA AATAGAATAC CTGCGAGAGA GGCAATTGAC TCATGAAGTG 
GAAGCTGAGG CAGTAAAGCG CAGGTTGGAG GACGAGGCCT GGTACCAGCA GCAAGAACTT 
CTCAAAGGGG CAGAGGAGCA GAGACGGAAG CTTCTTATGG ACGAGGAGCA AAAGCTTCTG 
CATCAGAGGC AGAGGCTGGC AACTGTAAAA CGGGAACTCA GACTGAAAGA ACTTCAGCTC 
CTGGATGCAG CCAGAAGGCG GTTTCTGAGG TACCAGCAGG ACCAGCGCAA AATGGAACTT 
AGAAGACTCG ACGATGAATT AGAGAGAAAG ATGAGTTTGC GAGAACGGGA AACTGCCACC 
ATAGCGAAAG ACGTCGAAAT CAGGCAGATG GAGCTTGAAG CACAAAGGAG ATTTTTTGAA 
CAGCAACTTG CTAAGGAGCA GGAGGCTGTG ACCCAGGAAG TCAAAGGAGA GATGGATGCC 
AACCGGAGAA GGGCCGACCT GGAAGAACAA ATGTTTTGGA GGCTTATGGA AACAGAGGAA 
GACCTTAAAG ATAAGAAGCT GCTTGAAGAG AGCCTTGCAA AGGCTGAACG TCTGTGTGTG 
GAAACCGACT GGAAAATACA AACCCTTCAG AAACAGAAAT GTGATGACCA GGAGCGAGGG 
AAGCGCTACG AAGAAGTGTC CAAAATGACT GACGATGTCC GAGAGAAAGA GCGAGAGTTA 
TGTGATGTTC TGAAAGCCAT GGAAACAAGA AAGTGGGCAG AGGTCTCTGA GAAAATGACT 
CAACTGGAAA CAGAGGAGCT TGCAAGCTCA GCGCTGCGGG CCAAAAGGAA TCAGTTCTTA 
CAGGAGAGGT TAAGGCAGGA AGCTGAACCT GTTAATATCT CAGAGGATGG AAATGAATAT 
TTTGAGAGAC TTCGCGATTT AAGCCGCAAT TCCACACAGA ATGATTTCAG TAGCAGCGCT 
GAGCGGCACG TAGCCGAAAA TGTTTGTTTA AACGATGTCT CTGCAAGTGA CTCCTCCACT 
CACTTTTCTT TAGACAGAGG AAGAGGGGAG CTGGAGAACA GAGAGCGAGC CCTGATATCA 
GAAGTACGAG AGCTCCGACA GAAACTTGCT ACCCAAGCAC GCAGAAAATA CCCTCAGTTG 
CACTTCTCTG AAACAAACTG GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945141.1
CDS	344..3610
Translation	MQTTDLGNKESGKIWHRKPNPSTNEGIIVNIVHVDASHPSKSARFLHAGFDSAGDSFIAGDHLGNVYLFNLNRNRFDLVQKTMQACTAIAFNLHRRTEFLVALADNSVKCFDTGAKELVSWMRGHESAVTSISVHPSGRYAVTTSSDTAQLWDLDTFQRKRKLNVRQSVGIQKVFFLPLSNTILSCFKDDSIFAWESDTLACKYQLPIPEDGTKLRYKAFAITRDGRMLAAGGKSNNLHLWCLDSKQLFRIIQMPTKVRSVQQLEFLPENFDGGSSQILGVLSQDGIMRFINIQTCKLVFDIGSHDNGIVTSSVSPNGRYITSVMENGSLNIYSVQALSKELNKPPPPLVKMVDLSKDKDSTGNKSGVSGASQEKVRVSSGRTCRPWKSKDQIVRTKYLRPEDTTTSEDKENTLPAGLSKQRLQALLKGFGEYPAKYRMFIWRSLLQLPENHAAFSSLLDKGTHTQYKLLHQEYPIKSRKLLRVLQRTLSALAHWSAIFGETKYLPLLAFPFVKLFQNNQLICFEVVATVITNWCQHWFEYFPNPPINILGMVENLLAHHDKELLQHFINYGVTSQVYAWPLLETLFSEVLTREEWLRLFDNVFSNHPSFLLMAVVSYIISSRSPLLHCNQKDDFEYFFHHRNNLDIGNMIREAYHLMDTSPAEIHPRRLLSDFEPLTRGQYPIFNKYPKFIVDYQGQERERIRQEEIEYLRERQLTHEVEAEAVKRRLEDEAWYQQQELLKGAEEQRRKLLMDEEQKLLHQRQRLATVKRELRLKELQLLDAARRRFLRYQQDQRKMELRRLDDELERKMSLRERETATIAKDVEIRQMELEAQRRFFEQQLAKEQEAVTQEVKGEMDANRRRADLEEQMFWRLMETEEDLKDKKLLEESLAKAERLCVETDWKIQTLQKQKCDDQERGKRYEEVSKMTDDVREKERELCDVLKAMETRKWAEVSEKMTQLETEELASSALRAKRNQFLQERLRQEAEPVNISEDGNEYFERLRDLSRNSTQNDFSSSAERHVAENVCLNDVSASDSSTHFSLDRGRGELENRERALISEVRELRQKLATQARRKYPQLHFSETNWT

Target ORF information:

RefSeq Version	XM_018089652.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089652.1

ORF Insert Sequence:

ATGCAAACCA CAGACTTGGG CAACAAAGAA TCTGGAAAGA TTTGGCACAG GAAGCCTAAC 
CCATCCACCA ACGAGGGGAT CATAGTGAAT ATTGTGCACG TTGATGCATC CCACCCCTCC 
AAGTCTGCAC GATTTCTCCA TGCTGGATTT GACAGCGCTG GGGACTCCTT CATAGCAGGA 
GATCACCTAG GAAATGTCTA TCTGTTCAAC TTAAACAGAA ACAGGTTTGA TCTTGTGCAG 
AAAACAATGC AGGCATGTAC AGCAATAGCA TTCAACCTGC ATCGGAGGAC AGAATTCCTT 
GTGGCACTTG CTGATAATTC AGTTAAGTGT TTTGACACAG GTGCAAAGGA GCTGGTTAGT 
TGGATGCGAG GCCACGAATC TGCTGTCACA TCCATTTCTG TACATCCATC TGGGAGATAT 
GCGGTTACCA CATCCAGCGA TACTGCCCAG CTCTGGGATT TGGATACTTT CCAAAGGAAA 
AGGAAACTCA ATGTTCGGCA GTCTGTGGGA ATACAAAAGG TATTCTTTCT TCCTTTAAGT 
AATACTATCT TGAGCTGCTT TAAAGATGAC TCCATCTTTG CCTGGGAAAG TGATACGCTT 
GCGTGCAAAT ACCAGTTACC TATTCCCGAA GATGGAACAA AGCTACGCTA TAAAGCTTTT 
GCCATAACCA GGGATGGGAG GATGCTGGCA GCCGGAGGAA AGTCAAACAA CCTTCATCTC 
TGGTGTCTGG ACTCCAAGCA GCTGTTCAGG ATCATACAGA TGCCCACTAA AGTCCGAAGT 
GTTCAGCAGC TGGAATTTCT GCCTGAAAAT TTTGATGGTG GATCCAGCCA GATTCTGGGA 
GTACTAAGTC AAGATGGAAT CATGAGGTTT ATCAACATCC AGACATGCAA ACTTGTGTTT 
GATATCGGCA GCCATGATAA TGGAATAGTG ACGTCGTCTG TAAGCCCAAA TGGGCGCTAT 
ATCACAAGTG TAATGGAGAA TGGCAGTTTG AACATATACA GTGTGCAAGC CCTAAGCAAA 
GAACTCAACA AGCCACCTCC ACCACTCGTA AAAATGGTCG ACTTATCCAA AGATAAAGAT 
TCTACTGGAA ACAAGTCAGG AGTGAGTGGA GCAAGCCAGG AAAAGGTCAG AGTGTCCTCA 
GGCAGAACAT GTAGGCCATG GAAGTCAAAG GATCAAATCG TAAGAACCAA GTACCTGAGA 
CCAGAAGATA CCACCACGAG TGAAGACAAA GAGAATACAC TACCAGCGGG ATTGAGCAAA 
CAGCGCCTAC AAGCTTTGTT GAAAGGATTT GGGGAATACC CTGCAAAATA CAGAATGTTT 
ATTTGGCGTT CGTTGCTCCA GCTGCCAGAG AACCATGCTG CTTTCAGTAG TTTACTTGAC 
AAAGGCACTC ATACTCAGTA CAAGCTTCTT CACCAGGAAT ATCCCATCAA AAGCAGGAAG 
CTCTTAAGGG TCCTTCAGAG GACACTATCA GCGCTGGCTC ATTGGTCGGC AATATTCGGA 
GAAACAAAAT ACCTGCCTCT GCTTGCTTTT CCCTTTGTCA AGTTGTTTCA GAACAATCAG 
CTCATTTGCT TTGAAGTGGT TGCAACAGTG ATTACTAATT GGTGCCAACA TTGGTTTGAA 
TATTTTCCAA ATCCCCCAAT TAACATTCTT GGCATGGTTG AGAATCTCCT GGCGCATCAC 
GACAAGGAAC TGCTTCAGCA TTTCATAAAT TATGGCGTGA CTTCTCAGGT TTATGCTTGG 
CCCCTTCTGG AAACCCTTTT CTCTGAGGTT CTGACAAGAG AGGAGTGGCT GAGGCTGTTT 
GACAACGTGT TTTCCAACCA TCCGTCATTC CTTTTGATGG CAGTCGTGTC TTATATCATC 
TCCTCCCGGT CGCCCCTGCT GCACTGTAAC CAAAAGGATG ACTTTGAGTA CTTCTTTCAT 
CACCGGAACA ATCTAGACAT CGGCAATATG ATCCGAGAAG CCTATCATCT TATGGACACA 
TCTCCTGCAG AAATCCATCC TCGGCGCTTG CTAAGTGACT TTGAACCTCT TACCAGAGGG 
CAATATCCCA TATTTAACAA ATACCCCAAA TTCATTGTGG ATTATCAGGG TCAGGAACGA 
GAGAGGATCA GGCAGGAGGA AATAGAATAC CTGCGAGAGA GGCAATTGAC TCATGAAGTG 
GAAGCTGAGG CAGTAAAGCG CAGGTTGGAG GACGAGGCCT GGTACCAGCA GCAAGAACTT 
CTCAAAGGGG CAGAGGAGCA GAGACGGAAG CTTCTTATGG ACGAGGAGCA AAAGCTTCTG 
CATCAGAGGC AGAGGCTGGC AACTGTAAAA CGGGAACTCA GACTGAAAGA ACTTCAGCTC 
CTGGATGCAG CCAGAAGGCG GTTTCTGAGG TACCAGCAGG ACCAGCGCAA AATGGAACTT 
AGAAGACTCG ACGATGAATT AGAGAGAAAG ATGAGTTTGC GAGAACGGGA AACTGCCACC 
ATAGCGAAAG ACGTCGAAAT CAGGCAGATG GAGCTTGAAG CACAAAGGAG ATTTTTTGAA 
CAGCAACTTG CTAAGGAGCA GGAGGCTGTG ACCCAGGAAG TCAAAGGAGA GATGGATGCC 
AACCGGAGAA GGGCCGACCT GGAAGAACAA ATGTTTTGGA GGCTTATGGA AACAGAGGAA 
GACCTTAAAG ATAAGAAGCT GCTTGAAGAG AGCCTTGCAA AGGCTGAACG TCTGTGTGTG 
GAAACCGACT GGAAAATACA AACCCTTCAG AAACAGAAAT GTGATGACCA GGAGCGAGGG 
AAGCGCTACG AAGAAGTGTC CAAAATGACT GACGATGTCC GAGAGAAAGA GCGAGAGTTA 
TGTGATGTTC TGAAAGCCAT GGAAACAAGA AAGTGGGCAG AGGTCTCTGA GAAAATGACT 
CAACTGGAAA CAGAGGAGCT TGCAAGCTCA GCGCTGCGGG CCAAAAGGAA TCAGTTCTTA 
CAGGAGAGGT TAAGGCAGGA AGCTGAACCT GTTAATATCT CAGAGGATGG AAATGAATAT 
TTTGAGAGAC TTCGCGATTT AAGCCGCAAT TCCACACAGA ATGATTTCAG TAGCAGCGCT 
GAGCGGCACG TAGCCGAAAA TGTTTGTTTA AACGATGTCT CTGCAAGTGA CTCCTCCACT 
CACTTTTCTT TAGACAGAGG AAGAGGGGAG CTGGAGAACA GAGAGCGAGC CCTGATATCA 
GAAGTACGAG AGCTCCGACA GAAACTTGCT ACCCAAGCAC GCAGAAAATA CCCTCAGTTG 
CACTTCTCTG AAACAAACTG GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d31 ( XM_018089651.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945140.1 Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa26723
Clone ID Related Accession (Same CDS sequence)	XM_018089652.1 , XM_018089651.1 , XM_012964537.2
Accession Version	XM_018089651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TBC1 domain family member 31 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683417.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA CAGACTTGGG CAACAAAGAA TCTGGAAAGA TTTGGCACAG GAAGCCTAAC 
CCATCCACCA ACGAGGGGAT CATAGTGAAT ATTGTGCACG TTGATGCATC CCACCCCTCC 
AAGTCTGCAC GATTTCTCCA TGCTGGATTT GACAGCGCTG GGGACTCCTT CATAGCAGGA 
GATCACCTAG GAAATGTCTA TCTGTTCAAC TTAAACAGAA ACAGGTTTGA TCTTGTGCAG 
AAAACAATGC AGGCATGTAC AGCAATAGCA TTCAACCTGC ATCGGAGGAC AGAATTCCTT 
GTGGCACTTG CTGATAATTC AGTTAAGTGT TTTGACACAG GTGCAAAGGA GCTGGTTAGT 
TGGATGCGAG GCCACGAATC TGCTGTCACA TCCATTTCTG TACATCCATC TGGGAGATAT 
GCGGTTACCA CATCCAGCGA TACTGCCCAG CTCTGGGATT TGGATACTTT CCAAAGGAAA 
AGGAAACTCA ATGTTCGGCA GTCTGTGGGA ATACAAAAGG TATTCTTTCT TCCTTTAAGT 
AATACTATCT TGAGCTGCTT TAAAGATGAC TCCATCTTTG CCTGGGAAAG TGATACGCTT 
GCGTGCAAAT ACCAGTTACC TATTCCCGAA GATGGAACAA AGCTACGCTA TAAAGCTTTT 
GCCATAACCA GGGATGGGAG GATGCTGGCA GCCGGAGGAA AGTCAAACAA CCTTCATCTC 
TGGTGTCTGG ACTCCAAGCA GCTGTTCAGG ATCATACAGA TGCCCACTAA AGTCCGAAGT 
GTTCAGCAGC TGGAATTTCT GCCTGAAAAT TTTGATGGTG GATCCAGCCA GATTCTGGGA 
GTACTAAGTC AAGATGGAAT CATGAGGTTT ATCAACATCC AGACATGCAA ACTTGTGTTT 
GATATCGGCA GCCATGATAA TGGAATAGTG ACGTCGTCTG TAAGCCCAAA TGGGCGCTAT 
ATCACAAGTG TAATGGAGAA TGGCAGTTTG AACATATACA GTGTGCAAGC CCTAAGCAAA 
GAACTCAACA AGCCACCTCC ACCACTCGTA AAAATGGTCG ACTTATCCAA AGATAAAGAT 
TCTACTGGAA ACAAGTCAGG AGTGAGTGGA GCAAGCCAGG AAAAGGTCAG AGTGTCCTCA 
GGCAGAACAT GTAGGCCATG GAAGTCAAAG GATCAAATCG TAAGAACCAA GTACCTGAGA 
CCAGAAGATA CCACCACGAG TGAAGACAAA GAGAATACAC TACCAGCGGG ATTGAGCAAA 
CAGCGCCTAC AAGCTTTGTT GAAAGGATTT GGGGAATACC CTGCAAAATA CAGAATGTTT 
ATTTGGCGTT CGTTGCTCCA GCTGCCAGAG AACCATGCTG CTTTCAGTAG TTTACTTGAC 
AAAGGCACTC ATACTCAGTA CAAGCTTCTT CACCAGGAAT ATCCCATCAA AAGCAGGAAG 
CTCTTAAGGG TCCTTCAGAG GACACTATCA GCGCTGGCTC ATTGGTCGGC AATATTCGGA 
GAAACAAAAT ACCTGCCTCT GCTTGCTTTT CCCTTTGTCA AGTTGTTTCA GAACAATCAG 
CTCATTTGCT TTGAAGTGGT TGCAACAGTG ATTACTAATT GGTGCCAACA TTGGTTTGAA 
TATTTTCCAA ATCCCCCAAT TAACATTCTT GGCATGGTTG AGAATCTCCT GGCGCATCAC 
GACAAGGAAC TGCTTCAGCA TTTCATAAAT TATGGCGTGA CTTCTCAGGT TTATGCTTGG 
CCCCTTCTGG AAACCCTTTT CTCTGAGGTT CTGACAAGAG AGGAGTGGCT GAGGCTGTTT 
GACAACGTGT TTTCCAACCA TCCGTCATTC CTTTTGATGG CAGTCGTGTC TTATATCATC 
TCCTCCCGGT CGCCCCTGCT GCACTGTAAC CAAAAGGATG ACTTTGAGTA CTTCTTTCAT 
CACCGGAACA ATCTAGACAT CGGCAATATG ATCCGAGAAG CCTATCATCT TATGGACACA 
TCTCCTGCAG AAATCCATCC TCGGCGCTTG CTAAGTGACT TTGAACCTCT TACCAGAGGG 
CAATATCCCA TATTTAACAA ATACCCCAAA TTCATTGTGG ATTATCAGGG TCAGGAACGA 
GAGAGGATCA GGCAGGAGGA AATAGAATAC CTGCGAGAGA GGCAATTGAC TCATGAAGTG 
GAAGCTGAGG CAGTAAAGCG CAGGTTGGAG GACGAGGCCT GGTACCAGCA GCAAGAACTT 
CTCAAAGGGG CAGAGGAGCA GAGACGGAAG CTTCTTATGG ACGAGGAGCA AAAGCTTCTG 
CATCAGAGGC AGAGGCTGGC AACTGTAAAA CGGGAACTCA GACTGAAAGA ACTTCAGCTC 
CTGGATGCAG CCAGAAGGCG GTTTCTGAGG TACCAGCAGG ACCAGCGCAA AATGGAACTT 
AGAAGACTCG ACGATGAATT AGAGAGAAAG ATGAGTTTGC GAGAACGGGA AACTGCCACC 
ATAGCGAAAG ACGTCGAAAT CAGGCAGATG GAGCTTGAAG CACAAAGGAG ATTTTTTGAA 
CAGCAACTTG CTAAGGAGCA GGAGGCTGTG ACCCAGGAAG TCAAAGGAGA GATGGATGCC 
AACCGGAGAA GGGCCGACCT GGAAGAACAA ATGTTTTGGA GGCTTATGGA AACAGAGGAA 
GACCTTAAAG ATAAGAAGCT GCTTGAAGAG AGCCTTGCAA AGGCTGAACG TCTGTGTGTG 
GAAACCGACT GGAAAATACA AACCCTTCAG AAACAGAAAT GTGATGACCA GGAGCGAGGG 
AAGCGCTACG AAGAAGTGTC CAAAATGACT GACGATGTCC GAGAGAAAGA GCGAGAGTTA 
TGTGATGTTC TGAAAGCCAT GGAAACAAGA AAGTGGGCAG AGGTCTCTGA GAAAATGACT 
CAACTGGAAA CAGAGGAGCT TGCAAGCTCA GCGCTGCGGG CCAAAAGGAA TCAGTTCTTA 
CAGGAGAGGT TAAGGCAGGA AGCTGAACCT GTTAATATCT CAGAGGATGG AAATGAATAT 
TTTGAGAGAC TTCGCGATTT AAGCCGCAAT TCCACACAGA ATGATTTCAG TAGCAGCGCT 
GAGCGGCACG TAGCCGAAAA TGTTTGTTTA AACGATGTCT CTGCAAGTGA CTCCTCCACT 
CACTTTTCTT TAGACAGAGG AAGAGGGGAG CTGGAGAACA GAGAGCGAGC CCTGATATCA 
GAAGTACGAG AGCTCCGACA GAAACTTGCT ACCCAAGCAC GCAGAAAATA CCCTCAGTTG 
CACTTCTCTG AAACAAACTG GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945140.1
CDS	537..3803
Translation	MQTTDLGNKESGKIWHRKPNPSTNEGIIVNIVHVDASHPSKSARFLHAGFDSAGDSFIAGDHLGNVYLFNLNRNRFDLVQKTMQACTAIAFNLHRRTEFLVALADNSVKCFDTGAKELVSWMRGHESAVTSISVHPSGRYAVTTSSDTAQLWDLDTFQRKRKLNVRQSVGIQKVFFLPLSNTILSCFKDDSIFAWESDTLACKYQLPIPEDGTKLRYKAFAITRDGRMLAAGGKSNNLHLWCLDSKQLFRIIQMPTKVRSVQQLEFLPENFDGGSSQILGVLSQDGIMRFINIQTCKLVFDIGSHDNGIVTSSVSPNGRYITSVMENGSLNIYSVQALSKELNKPPPPLVKMVDLSKDKDSTGNKSGVSGASQEKVRVSSGRTCRPWKSKDQIVRTKYLRPEDTTTSEDKENTLPAGLSKQRLQALLKGFGEYPAKYRMFIWRSLLQLPENHAAFSSLLDKGTHTQYKLLHQEYPIKSRKLLRVLQRTLSALAHWSAIFGETKYLPLLAFPFVKLFQNNQLICFEVVATVITNWCQHWFEYFPNPPINILGMVENLLAHHDKELLQHFINYGVTSQVYAWPLLETLFSEVLTREEWLRLFDNVFSNHPSFLLMAVVSYIISSRSPLLHCNQKDDFEYFFHHRNNLDIGNMIREAYHLMDTSPAEIHPRRLLSDFEPLTRGQYPIFNKYPKFIVDYQGQERERIRQEEIEYLRERQLTHEVEAEAVKRRLEDEAWYQQQELLKGAEEQRRKLLMDEEQKLLHQRQRLATVKRELRLKELQLLDAARRRFLRYQQDQRKMELRRLDDELERKMSLRERETATIAKDVEIRQMELEAQRRFFEQQLAKEQEAVTQEVKGEMDANRRRADLEEQMFWRLMETEEDLKDKKLLEESLAKAERLCVETDWKIQTLQKQKCDDQERGKRYEEVSKMTDDVREKERELCDVLKAMETRKWAEVSEKMTQLETEELASSALRAKRNQFLQERLRQEAEPVNISEDGNEYFERLRDLSRNSTQNDFSSSAERHVAENVCLNDVSASDSSTHFSLDRGRGELENRERALISEVRELRQKLATQARRKYPQLHFSETNWT

Target ORF information:

RefSeq Version	XM_018089651.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089651.1

ORF Insert Sequence:

ATGCAAACCA CAGACTTGGG CAACAAAGAA TCTGGAAAGA TTTGGCACAG GAAGCCTAAC 
CCATCCACCA ACGAGGGGAT CATAGTGAAT ATTGTGCACG TTGATGCATC CCACCCCTCC 
AAGTCTGCAC GATTTCTCCA TGCTGGATTT GACAGCGCTG GGGACTCCTT CATAGCAGGA 
GATCACCTAG GAAATGTCTA TCTGTTCAAC TTAAACAGAA ACAGGTTTGA TCTTGTGCAG 
AAAACAATGC AGGCATGTAC AGCAATAGCA TTCAACCTGC ATCGGAGGAC AGAATTCCTT 
GTGGCACTTG CTGATAATTC AGTTAAGTGT TTTGACACAG GTGCAAAGGA GCTGGTTAGT 
TGGATGCGAG GCCACGAATC TGCTGTCACA TCCATTTCTG TACATCCATC TGGGAGATAT 
GCGGTTACCA CATCCAGCGA TACTGCCCAG CTCTGGGATT TGGATACTTT CCAAAGGAAA 
AGGAAACTCA ATGTTCGGCA GTCTGTGGGA ATACAAAAGG TATTCTTTCT TCCTTTAAGT 
AATACTATCT TGAGCTGCTT TAAAGATGAC TCCATCTTTG CCTGGGAAAG TGATACGCTT 
GCGTGCAAAT ACCAGTTACC TATTCCCGAA GATGGAACAA AGCTACGCTA TAAAGCTTTT 
GCCATAACCA GGGATGGGAG GATGCTGGCA GCCGGAGGAA AGTCAAACAA CCTTCATCTC 
TGGTGTCTGG ACTCCAAGCA GCTGTTCAGG ATCATACAGA TGCCCACTAA AGTCCGAAGT 
GTTCAGCAGC TGGAATTTCT GCCTGAAAAT TTTGATGGTG GATCCAGCCA GATTCTGGGA 
GTACTAAGTC AAGATGGAAT CATGAGGTTT ATCAACATCC AGACATGCAA ACTTGTGTTT 
GATATCGGCA GCCATGATAA TGGAATAGTG ACGTCGTCTG TAAGCCCAAA TGGGCGCTAT 
ATCACAAGTG TAATGGAGAA TGGCAGTTTG AACATATACA GTGTGCAAGC CCTAAGCAAA 
GAACTCAACA AGCCACCTCC ACCACTCGTA AAAATGGTCG ACTTATCCAA AGATAAAGAT 
TCTACTGGAA ACAAGTCAGG AGTGAGTGGA GCAAGCCAGG AAAAGGTCAG AGTGTCCTCA 
GGCAGAACAT GTAGGCCATG GAAGTCAAAG GATCAAATCG TAAGAACCAA GTACCTGAGA 
CCAGAAGATA CCACCACGAG TGAAGACAAA GAGAATACAC TACCAGCGGG ATTGAGCAAA 
CAGCGCCTAC AAGCTTTGTT GAAAGGATTT GGGGAATACC CTGCAAAATA CAGAATGTTT 
ATTTGGCGTT CGTTGCTCCA GCTGCCAGAG AACCATGCTG CTTTCAGTAG TTTACTTGAC 
AAAGGCACTC ATACTCAGTA CAAGCTTCTT CACCAGGAAT ATCCCATCAA AAGCAGGAAG 
CTCTTAAGGG TCCTTCAGAG GACACTATCA GCGCTGGCTC ATTGGTCGGC AATATTCGGA 
GAAACAAAAT ACCTGCCTCT GCTTGCTTTT CCCTTTGTCA AGTTGTTTCA GAACAATCAG 
CTCATTTGCT TTGAAGTGGT TGCAACAGTG ATTACTAATT GGTGCCAACA TTGGTTTGAA 
TATTTTCCAA ATCCCCCAAT TAACATTCTT GGCATGGTTG AGAATCTCCT GGCGCATCAC 
GACAAGGAAC TGCTTCAGCA TTTCATAAAT TATGGCGTGA CTTCTCAGGT TTATGCTTGG 
CCCCTTCTGG AAACCCTTTT CTCTGAGGTT CTGACAAGAG AGGAGTGGCT GAGGCTGTTT 
GACAACGTGT TTTCCAACCA TCCGTCATTC CTTTTGATGG CAGTCGTGTC TTATATCATC 
TCCTCCCGGT CGCCCCTGCT GCACTGTAAC CAAAAGGATG ACTTTGAGTA CTTCTTTCAT 
CACCGGAACA ATCTAGACAT CGGCAATATG ATCCGAGAAG CCTATCATCT TATGGACACA 
TCTCCTGCAG AAATCCATCC TCGGCGCTTG CTAAGTGACT TTGAACCTCT TACCAGAGGG 
CAATATCCCA TATTTAACAA ATACCCCAAA TTCATTGTGG ATTATCAGGG TCAGGAACGA 
GAGAGGATCA GGCAGGAGGA AATAGAATAC CTGCGAGAGA GGCAATTGAC TCATGAAGTG 
GAAGCTGAGG CAGTAAAGCG CAGGTTGGAG GACGAGGCCT GGTACCAGCA GCAAGAACTT 
CTCAAAGGGG CAGAGGAGCA GAGACGGAAG CTTCTTATGG ACGAGGAGCA AAAGCTTCTG 
CATCAGAGGC AGAGGCTGGC AACTGTAAAA CGGGAACTCA GACTGAAAGA ACTTCAGCTC 
CTGGATGCAG CCAGAAGGCG GTTTCTGAGG TACCAGCAGG ACCAGCGCAA AATGGAACTT 
AGAAGACTCG ACGATGAATT AGAGAGAAAG ATGAGTTTGC GAGAACGGGA AACTGCCACC 
ATAGCGAAAG ACGTCGAAAT CAGGCAGATG GAGCTTGAAG CACAAAGGAG ATTTTTTGAA 
CAGCAACTTG CTAAGGAGCA GGAGGCTGTG ACCCAGGAAG TCAAAGGAGA GATGGATGCC 
AACCGGAGAA GGGCCGACCT GGAAGAACAA ATGTTTTGGA GGCTTATGGA AACAGAGGAA 
GACCTTAAAG ATAAGAAGCT GCTTGAAGAG AGCCTTGCAA AGGCTGAACG TCTGTGTGTG 
GAAACCGACT GGAAAATACA AACCCTTCAG AAACAGAAAT GTGATGACCA GGAGCGAGGG 
AAGCGCTACG AAGAAGTGTC CAAAATGACT GACGATGTCC GAGAGAAAGA GCGAGAGTTA 
TGTGATGTTC TGAAAGCCAT GGAAACAAGA AAGTGGGCAG AGGTCTCTGA GAAAATGACT 
CAACTGGAAA CAGAGGAGCT TGCAAGCTCA GCGCTGCGGG CCAAAAGGAA TCAGTTCTTA 
CAGGAGAGGT TAAGGCAGGA AGCTGAACCT GTTAATATCT CAGAGGATGG AAATGAATAT 
TTTGAGAGAC TTCGCGATTT AAGCCGCAAT TCCACACAGA ATGATTTCAG TAGCAGCGCT 
GAGCGGCACG TAGCCGAAAA TGTTTGTTTA AACGATGTCT CTGCAAGTGA CTCCTCCACT 
CACTTTTCTT TAGACAGAGG AAGAGGGGAG CTGGAGAACA GAGAGCGAGC CCTGATATCA 
GAAGTACGAG AGCTCCGACA GAAACTTGCT ACCCAAGCAC GCAGAAAATA CCCTCAGTTG 
CACTTCTCTG AAACAAACTG GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d31 ( XM_012964537.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819991.1 Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X2, mRNA.

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CloneID	OXa26723
Clone ID Related Accession (Same CDS sequence)	XM_018089652.1 , XM_018089651.1 , XM_012964537.2
Accession Version	XM_012964537.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TBC1 domain family member 31 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683417.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA CAGACTTGGG CAACAAAGAA TCTGGAAAGA TTTGGCACAG GAAGCCTAAC 
CCATCCACCA ACGAGGGGAT CATAGTGAAT ATTGTGCACG TTGATGCATC CCACCCCTCC 
AAGTCTGCAC GATTTCTCCA TGCTGGATTT GACAGCGCTG GGGACTCCTT CATAGCAGGA 
GATCACCTAG GAAATGTCTA TCTGTTCAAC TTAAACAGAA ACAGGTTTGA TCTTGTGCAG 
AAAACAATGC AGGCATGTAC AGCAATAGCA TTCAACCTGC ATCGGAGGAC AGAATTCCTT 
GTGGCACTTG CTGATAATTC AGTTAAGTGT TTTGACACAG GTGCAAAGGA GCTGGTTAGT 
TGGATGCGAG GCCACGAATC TGCTGTCACA TCCATTTCTG TACATCCATC TGGGAGATAT 
GCGGTTACCA CATCCAGCGA TACTGCCCAG CTCTGGGATT TGGATACTTT CCAAAGGAAA 
AGGAAACTCA ATGTTCGGCA GTCTGTGGGA ATACAAAAGG TATTCTTTCT TCCTTTAAGT 
AATACTATCT TGAGCTGCTT TAAAGATGAC TCCATCTTTG CCTGGGAAAG TGATACGCTT 
GCGTGCAAAT ACCAGTTACC TATTCCCGAA GATGGAACAA AGCTACGCTA TAAAGCTTTT 
GCCATAACCA GGGATGGGAG GATGCTGGCA GCCGGAGGAA AGTCAAACAA CCTTCATCTC 
TGGTGTCTGG ACTCCAAGCA GCTGTTCAGG ATCATACAGA TGCCCACTAA AGTCCGAAGT 
GTTCAGCAGC TGGAATTTCT GCCTGAAAAT TTTGATGGTG GATCCAGCCA GATTCTGGGA 
GTACTAAGTC AAGATGGAAT CATGAGGTTT ATCAACATCC AGACATGCAA ACTTGTGTTT 
GATATCGGCA GCCATGATAA TGGAATAGTG ACGTCGTCTG TAAGCCCAAA TGGGCGCTAT 
ATCACAAGTG TAATGGAGAA TGGCAGTTTG AACATATACA GTGTGCAAGC CCTAAGCAAA 
GAACTCAACA AGCCACCTCC ACCACTCGTA AAAATGGTCG ACTTATCCAA AGATAAAGAT 
TCTACTGGAA ACAAGTCAGG AGTGAGTGGA GCAAGCCAGG AAAAGGTCAG AGTGTCCTCA 
GGCAGAACAT GTAGGCCATG GAAGTCAAAG GATCAAATCG TAAGAACCAA GTACCTGAGA 
CCAGAAGATA CCACCACGAG TGAAGACAAA GAGAATACAC TACCAGCGGG ATTGAGCAAA 
CAGCGCCTAC AAGCTTTGTT GAAAGGATTT GGGGAATACC CTGCAAAATA CAGAATGTTT 
ATTTGGCGTT CGTTGCTCCA GCTGCCAGAG AACCATGCTG CTTTCAGTAG TTTACTTGAC 
AAAGGCACTC ATACTCAGTA CAAGCTTCTT CACCAGGAAT ATCCCATCAA AAGCAGGAAG 
CTCTTAAGGG TCCTTCAGAG GACACTATCA GCGCTGGCTC ATTGGTCGGC AATATTCGGA 
GAAACAAAAT ACCTGCCTCT GCTTGCTTTT CCCTTTGTCA AGTTGTTTCA GAACAATCAG 
CTCATTTGCT TTGAAGTGGT TGCAACAGTG ATTACTAATT GGTGCCAACA TTGGTTTGAA 
TATTTTCCAA ATCCCCCAAT TAACATTCTT GGCATGGTTG AGAATCTCCT GGCGCATCAC 
GACAAGGAAC TGCTTCAGCA TTTCATAAAT TATGGCGTGA CTTCTCAGGT TTATGCTTGG 
CCCCTTCTGG AAACCCTTTT CTCTGAGGTT CTGACAAGAG AGGAGTGGCT GAGGCTGTTT 
GACAACGTGT TTTCCAACCA TCCGTCATTC CTTTTGATGG CAGTCGTGTC TTATATCATC 
TCCTCCCGGT CGCCCCTGCT GCACTGTAAC CAAAAGGATG ACTTTGAGTA CTTCTTTCAT 
CACCGGAACA ATCTAGACAT CGGCAATATG ATCCGAGAAG CCTATCATCT TATGGACACA 
TCTCCTGCAG AAATCCATCC TCGGCGCTTG CTAAGTGACT TTGAACCTCT TACCAGAGGG 
CAATATCCCA TATTTAACAA ATACCCCAAA TTCATTGTGG ATTATCAGGG TCAGGAACGA 
GAGAGGATCA GGCAGGAGGA AATAGAATAC CTGCGAGAGA GGCAATTGAC TCATGAAGTG 
GAAGCTGAGG CAGTAAAGCG CAGGTTGGAG GACGAGGCCT GGTACCAGCA GCAAGAACTT 
CTCAAAGGGG CAGAGGAGCA GAGACGGAAG CTTCTTATGG ACGAGGAGCA AAAGCTTCTG 
CATCAGAGGC AGAGGCTGGC AACTGTAAAA CGGGAACTCA GACTGAAAGA ACTTCAGCTC 
CTGGATGCAG CCAGAAGGCG GTTTCTGAGG TACCAGCAGG ACCAGCGCAA AATGGAACTT 
AGAAGACTCG ACGATGAATT AGAGAGAAAG ATGAGTTTGC GAGAACGGGA AACTGCCACC 
ATAGCGAAAG ACGTCGAAAT CAGGCAGATG GAGCTTGAAG CACAAAGGAG ATTTTTTGAA 
CAGCAACTTG CTAAGGAGCA GGAGGCTGTG ACCCAGGAAG TCAAAGGAGA GATGGATGCC 
AACCGGAGAA GGGCCGACCT GGAAGAACAA ATGTTTTGGA GGCTTATGGA AACAGAGGAA 
GACCTTAAAG ATAAGAAGCT GCTTGAAGAG AGCCTTGCAA AGGCTGAACG TCTGTGTGTG 
GAAACCGACT GGAAAATACA AACCCTTCAG AAACAGAAAT GTGATGACCA GGAGCGAGGG 
AAGCGCTACG AAGAAGTGTC CAAAATGACT GACGATGTCC GAGAGAAAGA GCGAGAGTTA 
TGTGATGTTC TGAAAGCCAT GGAAACAAGA AAGTGGGCAG AGGTCTCTGA GAAAATGACT 
CAACTGGAAA CAGAGGAGCT TGCAAGCTCA GCGCTGCGGG CCAAAAGGAA TCAGTTCTTA 
CAGGAGAGGT TAAGGCAGGA AGCTGAACCT GTTAATATCT CAGAGGATGG AAATGAATAT 
TTTGAGAGAC TTCGCGATTT AAGCCGCAAT TCCACACAGA ATGATTTCAG TAGCAGCGCT 
GAGCGGCACG TAGCCGAAAA TGTTTGTTTA AACGATGTCT CTGCAAGTGA CTCCTCCACT 
CACTTTTCTT TAGACAGAGG AAGAGGGGAG CTGGAGAACA GAGAGCGAGC CCTGATATCA 
GAAGTACGAG AGCTCCGACA GAAACTTGCT ACCCAAGCAC GCAGAAAATA CCCTCAGTTG 
CACTTCTCTG AAACAAACTG GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819991.1
CDS	734..4000
Translation	MQTTDLGNKESGKIWHRKPNPSTNEGIIVNIVHVDASHPSKSARFLHAGFDSAGDSFIAGDHLGNVYLFNLNRNRFDLVQKTMQACTAIAFNLHRRTEFLVALADNSVKCFDTGAKELVSWMRGHESAVTSISVHPSGRYAVTTSSDTAQLWDLDTFQRKRKLNVRQSVGIQKVFFLPLSNTILSCFKDDSIFAWESDTLACKYQLPIPEDGTKLRYKAFAITRDGRMLAAGGKSNNLHLWCLDSKQLFRIIQMPTKVRSVQQLEFLPENFDGGSSQILGVLSQDGIMRFINIQTCKLVFDIGSHDNGIVTSSVSPNGRYITSVMENGSLNIYSVQALSKELNKPPPPLVKMVDLSKDKDSTGNKSGVSGASQEKVRVSSGRTCRPWKSKDQIVRTKYLRPEDTTTSEDKENTLPAGLSKQRLQALLKGFGEYPAKYRMFIWRSLLQLPENHAAFSSLLDKGTHTQYKLLHQEYPIKSRKLLRVLQRTLSALAHWSAIFGETKYLPLLAFPFVKLFQNNQLICFEVVATVITNWCQHWFEYFPNPPINILGMVENLLAHHDKELLQHFINYGVTSQVYAWPLLETLFSEVLTREEWLRLFDNVFSNHPSFLLMAVVSYIISSRSPLLHCNQKDDFEYFFHHRNNLDIGNMIREAYHLMDTSPAEIHPRRLLSDFEPLTRGQYPIFNKYPKFIVDYQGQERERIRQEEIEYLRERQLTHEVEAEAVKRRLEDEAWYQQQELLKGAEEQRRKLLMDEEQKLLHQRQRLATVKRELRLKELQLLDAARRRFLRYQQDQRKMELRRLDDELERKMSLRERETATIAKDVEIRQMELEAQRRFFEQQLAKEQEAVTQEVKGEMDANRRRADLEEQMFWRLMETEEDLKDKKLLEESLAKAERLCVETDWKIQTLQKQKCDDQERGKRYEEVSKMTDDVREKERELCDVLKAMETRKWAEVSEKMTQLETEELASSALRAKRNQFLQERLRQEAEPVNISEDGNEYFERLRDLSRNSTQNDFSSSAERHVAENVCLNDVSASDSSTHFSLDRGRGELENRERALISEVRELRQKLATQARRKYPQLHFSETNWT

Target ORF information:

RefSeq Version	XM_012964537.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TBC1 domain family member 31 (tbc1d31), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964537.2

ORF Insert Sequence:

ATGCAAACCA CAGACTTGGG CAACAAAGAA TCTGGAAAGA TTTGGCACAG GAAGCCTAAC 
CCATCCACCA ACGAGGGGAT CATAGTGAAT ATTGTGCACG TTGATGCATC CCACCCCTCC 
AAGTCTGCAC GATTTCTCCA TGCTGGATTT GACAGCGCTG GGGACTCCTT CATAGCAGGA 
GATCACCTAG GAAATGTCTA TCTGTTCAAC TTAAACAGAA ACAGGTTTGA TCTTGTGCAG 
AAAACAATGC AGGCATGTAC AGCAATAGCA TTCAACCTGC ATCGGAGGAC AGAATTCCTT 
GTGGCACTTG CTGATAATTC AGTTAAGTGT TTTGACACAG GTGCAAAGGA GCTGGTTAGT 
TGGATGCGAG GCCACGAATC TGCTGTCACA TCCATTTCTG TACATCCATC TGGGAGATAT 
GCGGTTACCA CATCCAGCGA TACTGCCCAG CTCTGGGATT TGGATACTTT CCAAAGGAAA 
AGGAAACTCA ATGTTCGGCA GTCTGTGGGA ATACAAAAGG TATTCTTTCT TCCTTTAAGT 
AATACTATCT TGAGCTGCTT TAAAGATGAC TCCATCTTTG CCTGGGAAAG TGATACGCTT 
GCGTGCAAAT ACCAGTTACC TATTCCCGAA GATGGAACAA AGCTACGCTA TAAAGCTTTT 
GCCATAACCA GGGATGGGAG GATGCTGGCA GCCGGAGGAA AGTCAAACAA CCTTCATCTC 
TGGTGTCTGG ACTCCAAGCA GCTGTTCAGG ATCATACAGA TGCCCACTAA AGTCCGAAGT 
GTTCAGCAGC TGGAATTTCT GCCTGAAAAT TTTGATGGTG GATCCAGCCA GATTCTGGGA 
GTACTAAGTC AAGATGGAAT CATGAGGTTT ATCAACATCC AGACATGCAA ACTTGTGTTT 
GATATCGGCA GCCATGATAA TGGAATAGTG ACGTCGTCTG TAAGCCCAAA TGGGCGCTAT 
ATCACAAGTG TAATGGAGAA TGGCAGTTTG AACATATACA GTGTGCAAGC CCTAAGCAAA 
GAACTCAACA AGCCACCTCC ACCACTCGTA AAAATGGTCG ACTTATCCAA AGATAAAGAT 
TCTACTGGAA ACAAGTCAGG AGTGAGTGGA GCAAGCCAGG AAAAGGTCAG AGTGTCCTCA 
GGCAGAACAT GTAGGCCATG GAAGTCAAAG GATCAAATCG TAAGAACCAA GTACCTGAGA 
CCAGAAGATA CCACCACGAG TGAAGACAAA GAGAATACAC TACCAGCGGG ATTGAGCAAA 
CAGCGCCTAC AAGCTTTGTT GAAAGGATTT GGGGAATACC CTGCAAAATA CAGAATGTTT 
ATTTGGCGTT CGTTGCTCCA GCTGCCAGAG AACCATGCTG CTTTCAGTAG TTTACTTGAC 
AAAGGCACTC ATACTCAGTA CAAGCTTCTT CACCAGGAAT ATCCCATCAA AAGCAGGAAG 
CTCTTAAGGG TCCTTCAGAG GACACTATCA GCGCTGGCTC ATTGGTCGGC AATATTCGGA 
GAAACAAAAT ACCTGCCTCT GCTTGCTTTT CCCTTTGTCA AGTTGTTTCA GAACAATCAG 
CTCATTTGCT TTGAAGTGGT TGCAACAGTG ATTACTAATT GGTGCCAACA TTGGTTTGAA 
TATTTTCCAA ATCCCCCAAT TAACATTCTT GGCATGGTTG AGAATCTCCT GGCGCATCAC 
GACAAGGAAC TGCTTCAGCA TTTCATAAAT TATGGCGTGA CTTCTCAGGT TTATGCTTGG 
CCCCTTCTGG AAACCCTTTT CTCTGAGGTT CTGACAAGAG AGGAGTGGCT GAGGCTGTTT 
GACAACGTGT TTTCCAACCA TCCGTCATTC CTTTTGATGG CAGTCGTGTC TTATATCATC 
TCCTCCCGGT CGCCCCTGCT GCACTGTAAC CAAAAGGATG ACTTTGAGTA CTTCTTTCAT 
CACCGGAACA ATCTAGACAT CGGCAATATG ATCCGAGAAG CCTATCATCT TATGGACACA 
TCTCCTGCAG AAATCCATCC TCGGCGCTTG CTAAGTGACT TTGAACCTCT TACCAGAGGG 
CAATATCCCA TATTTAACAA ATACCCCAAA TTCATTGTGG ATTATCAGGG TCAGGAACGA 
GAGAGGATCA GGCAGGAGGA AATAGAATAC CTGCGAGAGA GGCAATTGAC TCATGAAGTG 
GAAGCTGAGG CAGTAAAGCG CAGGTTGGAG GACGAGGCCT GGTACCAGCA GCAAGAACTT 
CTCAAAGGGG CAGAGGAGCA GAGACGGAAG CTTCTTATGG ACGAGGAGCA AAAGCTTCTG 
CATCAGAGGC AGAGGCTGGC AACTGTAAAA CGGGAACTCA GACTGAAAGA ACTTCAGCTC 
CTGGATGCAG CCAGAAGGCG GTTTCTGAGG TACCAGCAGG ACCAGCGCAA AATGGAACTT 
AGAAGACTCG ACGATGAATT AGAGAGAAAG ATGAGTTTGC GAGAACGGGA AACTGCCACC 
ATAGCGAAAG ACGTCGAAAT CAGGCAGATG GAGCTTGAAG CACAAAGGAG ATTTTTTGAA 
CAGCAACTTG CTAAGGAGCA GGAGGCTGTG ACCCAGGAAG TCAAAGGAGA GATGGATGCC 
AACCGGAGAA GGGCCGACCT GGAAGAACAA ATGTTTTGGA GGCTTATGGA AACAGAGGAA 
GACCTTAAAG ATAAGAAGCT GCTTGAAGAG AGCCTTGCAA AGGCTGAACG TCTGTGTGTG 
GAAACCGACT GGAAAATACA AACCCTTCAG AAACAGAAAT GTGATGACCA GGAGCGAGGG 
AAGCGCTACG AAGAAGTGTC CAAAATGACT GACGATGTCC GAGAGAAAGA GCGAGAGTTA 
TGTGATGTTC TGAAAGCCAT GGAAACAAGA AAGTGGGCAG AGGTCTCTGA GAAAATGACT 
CAACTGGAAA CAGAGGAGCT TGCAAGCTCA GCGCTGCGGG CCAAAAGGAA TCAGTTCTTA 
CAGGAGAGGT TAAGGCAGGA AGCTGAACCT GTTAATATCT CAGAGGATGG AAATGAATAT 
TTTGAGAGAC TTCGCGATTT AAGCCGCAAT TCCACACAGA ATGATTTCAG TAGCAGCGCT 
GAGCGGCACG TAGCCGAAAA TGTTTGTTTA AACGATGTCT CTGCAAGTGA CTCCTCCACT 
CACTTTTCTT TAGACAGAGG AAGAGGGGAG CTGGAGAACA GAGAGCGAGC CCTGATATCA 
GAAGTACGAG AGCTCCGACA GAAACTTGCT ACCCAAGCAC GCAGAAAATA CCCTCAGTTG 
CACTTCTCTG AAACAAACTG GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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tbc1d31 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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