ASS1 cDNA ORF clone, Bos taurus(cattle)
| Gene Symbol | ASS1 |
| Entrez Gene ID | 280726 |
| Full Name | argininosuccinate synthase 1 |
| Synonyms | ASS,CN |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Bos taurus(cattle) |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_173892.4 | NP_776317.1 | argininosuccinate synthase |
| Mus musculus (house mouse) | Ass1 | NP_031520.1 |
| Arabidopsis thaliana (thale cress) | AT4G24830 | NP_194214.2 |
| Homo sapiens (human) | ASS1 | NP_446464.1 |
| LOC716168 | XP_001106949.2 | |
| Canis lupus familiaris (dog) | ASS1 | XP_005625292.1 |
| Rattus norvegicus (Norway rat) | Ass1 | NP_037289.1 |
| Kluyveromyces lactis | KLLA0C04037g | XP_452377.1 |
| Magnaporthe oryzae (rice blast fungus) | MGG_15868 | XP_003715367.1 |
| Oryza sativa (rice) | Os12g0235800 | NP_001066459.1 |
| Xenopus tropicalis (tropical clawed frog) | ass1 | NP_001011212.1 |
| Pan troglodytes (chimpanzee) | ASS1 | XP_003951602.1 |
| Drosophila melanogaster (fruit fly) | CG1315 | NP_649674.1 |
| Eremothecium gossypii | AGOS_AER230C | NP_985087.2 |
| Bos taurus (cattle) | ASS1 | NP_776317.1 |
| Gallus gallus (chicken) | ASS1 | NP_001013413.1 |
| Danio rerio (zebrafish) | ass1 | NP_001004603.1 |
| Anopheles gambiae (African malaria mosquito) | ASSY_ANOGA | XP_311863.3 |
| Saccharomyces cerevisiae (baker's yeast) | ARG1 | NP_014583.2 |
| Schizosaccharomyces pombe (fission yeast) | arg12 | NP_595183.1 |
| Neurospora crassa | NCU02639 | XP_963976.1 |
Related articles in PubMed
Protein kinase C? phosphorylates a novel argininosuccinate synthase site at serine 328 during calcium-dependent stimulation of endothelial nitric-oxide synthase in vascular endothelial cells.
Haines RJ, Corbin KD, Pendleton LC, Eichler DC
The Journal of biological chemistry287(31)26168-76(2012 Jul)
A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)
Phosphorylation of argininosuccinate synthase by protein kinase A.
Corbin KD, Pendleton LC, Solomonson LP, Eichler DC
Biochemical and biophysical research communications377(4)1042-6(2008 Dec)
Tumor necrosis factor-alpha reduces argininosuccinate synthase expression and nitric oxide production in aortic endothelial cells.
Goodwin BL, Pendleton LC, Levy MM, Solomonson LP, Eichler DC
American journal of physiology. Heart and circulatory physiology293(2)H1115-21(2007 Aug)
Regulation of endothelial argininosuccinate synthase expression and NO production by an upstream open reading frame.
Pendleton LC, Goodwin BL, Solomonson LP, Eichler DC
The Journal of biological chemistry280(25)24252-60(2005 Jun)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
calcium-dependent phosphorylation of argininosuccinate synthase Ser-328 is mediated by PKCalpha
Title: Protein kinase C? phosphorylates a novel argininosuccinate synthase site at serine 328 during calcium-dependent stimulation of endothelial nitric-oxide synthase in vascular endothelial cells.
These results represent the first demonstration that vascular endothelial nitric oxide production can be regulated by dynamic argininosuccinate synthase phosphorylation.
Title: Phosphorylation of argininosuccinate synthase by protein kinase A.
In endothelial cells TNF-alpha coordinately downregulates nitric oxide synthase (eNOS) and argininosuccinate synthase expression, resulting in a severely impaired citrulline-NO cycle.
Title: Tumor necrosis factor-alpha reduces argininosuccinate synthase expression and nitric oxide production in aortic endothelial cells.
the function of the extended 5'-UTRs of AS mRNA was investigated
Title: Regulation of endothelial argininosuccinate synthase expression and NO production by an upstream open reading frame.
dose-dependent reduction of ASS as a result of siRNA treatment corresponded to diminished capacity to produce NO, despite saturating levels of arginine in the medium; reduced expression of ASS resulted in loss of viability due to apoptosis
Title: Argininosuccinate synthase expression is required to maintain nitric oxide production and cell viability in aortic endothelial cells.
The following ASS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OBa44680 | |
| Clone ID Related Accession (Same CDS sequence) | NM_173892.4 | |
| Accession Version | NM_173892.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-19 | |
| Organism | Bos taurus(cattle) | |
| Product | argininosuccinate synthase | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC102474.1. On Aug 30, 2012 this sequence version replaced NM_173892.3. ##Evidence-Data-START## Transcript exon combination :: BC102474.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGTCCGGCA AAGGCTCCGT GGTTCTGGCC TACAGTGGGG GCCTGGACAC CTCCTGCATC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_776317.1 |
| CDS | 44..1282 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_173892.4 |
ORF Insert Sequence:
1 | ATGTCCGGCA AAGGCTCCGT GGTTCTGGCC TACAGTGGGG GCCTGGACAC CTCCTGCATC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
Protein kinase C? phosphorylates a novel argininosuccinate synthase site at serine 328 during calcium-dependent stimulation of endothelial nitric-oxide synthase in vascular endothelial cells. |
|
A whole-genome assembly of the domestic cow, Bos taurus. |
|
Phosphorylation of argininosuccinate synthase by protein kinase A. |
|
Tumor necrosis factor-alpha reduces argininosuccinate synthase expression and nitric oxide production in aortic endothelial cells. |
|
Regulation of endothelial argininosuccinate synthase expression and NO production by an upstream open reading frame. |