MYH10 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
NM_174834.1	NP_777259.1	myosin-10
XM_005220297.3	XP_005220354.1	myosin-10 isoform X1
XM_015458671.1	XP_015314157.1	myosin-10 isoform X3
XM_005220298.4	XP_005220355.1	myosin-10 isoform X1
XM_005220299.4	XP_005220356.1	myosin-10 isoform X2
XM_015458672.1	XP_015314158.1	myosin-10 isoform X4
XM_015458671.2	XP_015314157.1	myosin-10 isoform X3
XM_015458670.1	XP_015314156.1	myosin-10 isoform X1
XM_005220297.4	XP_005220354.1	myosin-10 isoform X1
XM_005220298.3	XP_005220355.1	myosin-10 isoform X1
XM_005220299.3	XP_005220356.1	myosin-10 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa226016	XM_005220299.4 Latest version!	Bos taurus myosin heavy chain 10 (MYH10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa226017	XM_015458671.2 Latest version!	Bos taurus myosin heavy chain 10 (MYH10), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa226015	XM_005220298.4 Latest version!	Bos taurus myosin heavy chain 10 (MYH10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa226015	XM_005220297.4 Latest version!	Bos taurus myosin heavy chain 10 (MYH10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa43707	NM_174834.1 Latest version!	Bos taurus myosin heavy chain 10 (MYH10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa29701	XM_005220297.3 Latest version!	Bos taurus myosin, heavy chain 10, non-muscle (MYH10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa29701	XM_015458670.1 Latest version!	Bos taurus myosin, heavy chain 10, non-muscle (MYH10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa29701	XM_005220298.3 Latest version!	Bos taurus myosin, heavy chain 10, non-muscle (MYH10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa29702	XM_005220299.3 Latest version!	Bos taurus myosin, heavy chain 10, non-muscle (MYH10), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa29703	XM_015458671.1 Latest version!	Bos taurus myosin, heavy chain 10, non-muscle (MYH10), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa93841	XM_015458672.1 Latest version!	Bos taurus myosin, heavy chain 10, non-muscle (MYH10), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

MYH10 ( XM_005220299.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220356.1 Bos taurus myosin heavy chain 10 (MYH10), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa226016
Clone ID Related Accession (Same CDS sequence)	XM_005220299.4
Accession Version	XM_005220299.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myosin-10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220299.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTGGGGAAC TTGAACGGCA GCTTTTGCAA 
GCAAATCCAA TCCTGGAATC ATTTGGAAAC GCAAAGACTG TGAAAAATGA TAACTCATCT 
CGTTTTGGCA AGTTTATTCG GATCAACTTT GATGTAACTG GCTATATCGT TGGGGCCAAC 
ATTGAAACAT ACCTTCTGGA AAAATCTCGT GCTGTTCGTC AAGCAAAAGA TGAGCGTACT 
TTTCATATCT TTTACCAATT GTTATCTGGA GCAGGAGAAC ATCTGAAGTC TGATTTGCTC 
CTTGAAGGAT TTAATAACTA CAGATTTCTC TCCAATGGCT ATATTCCTAT TCCGGGACAA 
CAAGACAAAG ATAACTTCCA GGAGACAATG GAGGCAATGC ATATAATGGG CTTCTCCCAT 
GAAGAGATTC TGTCAATGCT TAAAGTGGTA TCTTCAGTGC TTCAGTTTGG AAATATTTCT 
TTCAAAAAGG AGCGAAACAC TGATCAAGCC TCCATGCCGG AAAATACAGT TGCACAGAAG 
CTCTGCCATC TTCTTGGCAT GAATGTGATG GAGTTCACTC GGGCCATTCT CACCCCCCGG 
ATCAAGGTCG GCCGAGACTA CGTGCAGAAA GCCCAGACCA AAGAACAAGC AGATTTTGCA 
GTAGAAGCTT TGGCAAAAGC TACCTATGAG CGGCTCTTCC GCTGGCTCGT TCACCGCATC 
AATAAAGCTC TGGACAGGAC CAAACGGCAA GGGGCCTCCT TCATTGGGAT CCTGGACATC 
GCTGGATTTG AGATTTTTGA GCTGAACTCA TTCGAGCAAC TGTGCATCAA CTACACCAAC 
GAGAAGCTGC AGCAGCTCTT CAACCACACC ATGTTCATCC TGGAGCAGGA GGAGTACCAG 
CGCGAGGGCA TCGAGTGGAA CTTCATCGAC TTTGGCCTCG ACCTGCAGCC CTGCATTGAC 
CTCATCGAGA GACCTGCTAA CCCTCCTGGC GTGCTGGCCC TTCTGGATGA AGAGTGCTGG 
TTCCCTAAAG CCACAGATAA GACCTTTGTT GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC 
TCCAAGTTTC AGAAACCACG TCAATTGAAA GACAAAGCTG ATTTTTGCAT TATCCATTAT 
GCAGGGAAGG TGGACTACAA GGCAGACGAG TGGCTGATGA AGAACATGGA CCCCCTGAAT 
GACAACGTGG CCACCCTCCT GCACCAGTCC TCTGACAGAT TCGTGGCAGA GCTCTGGAAG 
GATGAGATTC AGAATATTCA GAGAGCTTCT TTCTATGACA GTGTTTCTGG TCTTCATGAG 
CCACCAGTGG ACCGGATCGT GGGTCTGGAT CAGGTCACGG GGATGACGGA GACGGCATTC 
GGCTCCGCCT ATAAAACCAA GAAGGGCATG TTCCGCACTG TCGGGCAGCT CTACAAAGAA 
TCGCTCACCA AGCTGATGGC GACGCTCCGG AACACCAACC CGAACTTCGT GCGCTGCATC 
ATCCCAAATC ACGAGAAGCG GGCTGGGAAA CTGGACCCAC ACCTGGTGCT GGATCAGCTC 
CGCTGCAATG GCGTCCTGGA AGGGATCCGC ATCTGCCGCC AGGGCTTCCC CAACCGGATC 
GTTTTCCAGG AATTCAGACA GAGATACGAG ATCCTCACTC CAAATGCTAT CCCTAAGGGT 
TTCATGGATG GCAAACAGGC TTGTGAACGA ATGATCCGGG CTTTAGAATT GGACCCAAAC 
TTGTACAGAA TTGGACAGAG CAAGATATTT TTCAGAGCTG GAGTCTTGGC ACACTTAGAG 
GAAGAACGAG ATTTAAAAAT TACCGACATC ATTATCTTCT TCCAGGCTGT TTGCAGGGGT 
TATTTAGCCA GAAAGGCCTT CGCCAAGAAG CAGCAGCAGC TGAGTGCCCT GAAGGTGCTG 
CAGCGCAACT GTGCCGCGTA CCTGAAGCTG CGGCACTGGC AGTGGTGGCG CGTCTTCACC 
AAGGTGAAAC CACTCCTACA AGTCACTCGT CAAGAGGAGG AGCTCCAGGC CAAAGATGAA 
GAGCTGCTGA AGGTGAAAGA GAAGCAGACG AAGGTGGAAG GGGAGCTGGA GGAGATGGAG 
AGGAAGCACC AACAGCTGCT GGAGGAGAAG AATATCCTGG CAGAGCAGCT GCAGGCGGAG 
ACCGAGCTCT TTGCCGAAGC AGAGGAGATG AGGGCCAGAC TTGCCGCTAA GAAGCAGGAA 
TTAGAAGAGA TTCTCCATGA CTTGGAATCT AGGGTAGAAG AAGAAGAAGA AAGAAACCAA 
ATCCTCCAAA ATGAAAAGAA GAAAATGCAA GCACACATTC AGGACCTGGA GGAGCAGCTA 
GATGAGGAGG AGGGGGCTCG CCAGAAGCTG CAGCTGGAGA AGGTGACGGC GGAGGCCAAG 
ATCAAGAAGA TGGAAGAAGA GATTCTGCTT CTGGAGGACC AGAACTCCAA GTTTATCAAA 
GAAAAGAAAC TCATGGAAGA TCGCATTGCA GAATGTTCCT CTCAGCTGGC TGAAGAGGAA 
GAAAAGGCAA AAAACTTGGC CAAAATCAGG AATAAGCAAG AAGTAATGAT CTCGGATTTA 
GAAGAGCGCT TAAAGAAGGA GGAGAAGACG CGCCAGGAGC TGGAGAAGGC CAAAAGGAAA 
CTGGACGGGG AGACCACGGA CCTGCAAGAC CAGATCGCCG AGCTCCAGGC GCAGATTGAC 
GAGCTTAAGA TCCAGGTGGC CAAGAAAGAG GAGGAGCTGC AGGGCGCCCT GGCCAGGGGT 
GACGACGAGA CGCTACACAA GAACAACGCA CTAAAAGTCG TGCGAGAGCT GCAGGCCCAG 
ATCGCCGAGC TGCAGGAGGA CTTTGAATCT GAGAAGGCCT CGCGGAACAA AGCAGAGAAG 
CAGAAGAGGG ACTTGAGTGA GGAGCTGGAG GCACTCAAGA CGGAGCTGGA GGACACGCTG 
GACACCACGG CGGCCCAGCA GGAACTGCGT ACAAAACGAG AACAAGAAGT GGCGGAGCTG 
AAGAAGGCTC TCGAGGAGGA AACGAAGAGC CACGAGGCTC AGATCCAGGA CATGAGGCAG 
AGGCATGCGA CCGCCTTGGA GGAGCTGTCG GAGCAGCTGG AGCAGGCCAA GAGGTTCAAA 
GCAAATCTGG AAAAGAACAA GCAGGGCCTG GAGACAGACA ACAAGGAGCT GGCGTGCGAG 
GTCAAGGTGC TGCAGCAGGT CAAGGCTGAG TCCGAGCACA AGAGGAAGAA GCTGGACGCC 
CAGGTCCAGG AGCTCCACGC CAAGGTCTCA GAGGGAGACA GACTCAGGGT GGAGCTGGCA 
GAGAAAGCCA ATAAGTTACA GAATGAATTG GATAATGTCT CAACTCTTCT AGAAGAAGCA 
GAGAAGAAGG GTATTAAATT TGCTAAGGAT GCAGCTGGTC TGGAGTCTCA GCTCCAAGAC 
ACACAGGAGC TTCTTCAGGA GGAGACGCGC CAGAAACTGA ACCTGAGCAG TCGGATCCGG 
CAGCTGGAGG AGGAGAGGAG CAGCCTCCAG GAGCAGCAGG AGGAAGAGGA GGAGGCCAGG 
AGGAGCCTGG AGAAGCAGCT GCAGGCCCTG CAGGCCCAGT TGACGGATAC CAAGAAGAAA 
GTAGATGATG ACCTGGGGAC AATTGAAAAT TTGGAAGAAG CCAAGAAGAA GCTCCTCAAG 
GATGTGGAGG TGCTGAGCCA GCGCCTGGAG GAGAAGGCCC TGGCCTATGA CAAGCTGGAG 
AAGACCAAGA CCCGCCTGCA GCAGGAGCTG GATGACCTGC TGGTAGACCT GGACCACCAG 
CGCCAGATCG TCTCCAACTT GGAGAAGAAG CAGAAGAAGT TTGATCAGCT GTTAGCGGAA 
GAGAAGAACA TCTCTGCCCG CTACGCAGAA GAGCGCGACC GGGCAGAGGC AGAGGCCCGA 
GAGAAGGAGA CCAAAGCCCT GTCCCTGGCC CGCGCCCTGG AGGAGGCCCT GGAGGCCAGG 
GAGGAGGCTG AGCGACAGAA TAAGCAGCTG CGGGCGGACA TGGAGGACCT CATGAGCTCC 
AAGGACGATG TGGGCAAGAA CGTTCATGAA CTTGAGAAAT CCAAACGGGC CCTAGAGCAG 
CAGGTGGAGG AAATGAGGAC CCAACTGGAG GAGCTGGAAG ATGAGCTCCA GGCTACAGAA 
GACGCCAAGC TTCGCCTAGA GGTCAACATG CAAGCCATGA AAGCCCAGTT TGAGAGAGAC 
TTGCAGACCA GGGATGAGCA GAATGAGGAA AAGAAGCGGC TGCTCATTAA ACAGGTGCGG 
GAGCTTGAGG CAGAGCTGGA GGATGAGCGG AAACAGCGGG CCCTGGCTGT TGCCTCGAAG 
AAGAAAATGG AAATAGACCT GAAGGACCTG GAGGCTCAAA TCGAGGCAGC TAACAAAGCA 
AGGGATGAAG TGATCAAGCA GCTCCGCAAA CTCCAGGCTC AGATGAAGGA CTACCAGCGT 
GAATTAGAAG AAGCTCGTGC ATCCCGGGAT GAGATTTTTG CTCAATCCAA AGAGAGTGAG 
AAGAAACTGA AAAGCCTGGA AGCAGAAATC CTTCAGTTGC AGGAGGAACT TGCCTCATCC 
GAGCGAGCCC GCCGACATGC TGAGCAGGAG AGAGACGAGC TGGCGGACGA GATTGCCAAT 
AGCGCCTCCG GCAAGTCTGC ACTCCTGGAT GAGAAGCGGC GTCTGGAGGC TCGGATCGCA 
CAGCTAGAGG AGGAGCTGGA GGAGGAGCAG AGCAACATGG AGCTGCTCAA TGACCGCTTC 
CGCAAGACCA CACTGCAGGT GGACACGCTG AACACTGAGC TGGCGGCCGA GCGCAGCGCT 
GCCCAGAAGA GCGATAATGC GCGCCAGCAG CTGGAGCGGC AGAACAAGGA GTTGAAGGCC 
AAGCTGCAGG AGCTGGAAGG CGCTGTCAAG TCCAAGTTCA AGGCCACCAT CTCTGCTCTA 
GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG CTTGAGCAGG AAGCCAAGGA AAGAGCCGCT 
GCCAATAAAC TAGTCCGTCG TACCGAGAAG AAGCTGAAAG AAATCTTCAT GCAGGTTGAA 
GATGAACGCC GCCATGCTGA CCAGTATAAA GAACAGATGG AGAAGGCCAA TGCCAGGATG 
AAGCAGCTCA AGCGGCAGCT GGAGGAAGCA GAGGAAGAAG CTACACGTGC CAACGCATCT 
CGGCGCAAAC TCCAGCGGGA ACTGGATGAT GCCACTGAGG CCAACGAGGG CCTGAGCCGC 
GAGGTCAGCA CCCTGAAGAA CCGGCTGAGG CGAGGCGGCC CGATCAGCTT CTCCTCCAGC 
CGATCAGGCC GGCGCCAGCT GCACATTGAG GGCGCGTCCC TGGAGCTGTC GGACGACGAC 
ACAGAAAGTA AGACCAGTGA CATCAACGAG ACACAGCCGC CGCAGTCCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220356.1
CDS	309..6302
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVLVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDEIQNIQRASFYDSVSGLHEPPVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKEKQTKVEGELEEMERKHQQLLEEKNILAEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKIQVAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETKSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSTLLEEAEKKGIKFAKDAAGLESQLQDTQELLQEETRQKLNLSSRIRQLEEERSSLQEQQEEEEEARRSLEKQLQALQAQLTDTKKKVDDDLGTIENLEEAKKKLLKDVEVLSQRLEEKALAYDKLEKTKTRLQQELDDLLVDLDHQRQIVSNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALEAREEAERQNKQLRADMEDLMSSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLIKQVRELEAELEDERKQRALAVASKKKMEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNTELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDINETQPPQSE

Target ORF information:

RefSeq Version	XM_005220299.4
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin heavy chain 10 (MYH10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220299.4

ORF Insert Sequence:

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTGGGGAAC TTGAACGGCA GCTTTTGCAA 
GCAAATCCAA TCCTGGAATC ATTTGGAAAC GCAAAGACTG TGAAAAATGA TAACTCATCT 
CGTTTTGGCA AGTTTATTCG GATCAACTTT GATGTAACTG GCTATATCGT TGGGGCCAAC 
ATTGAAACAT ACCTTCTGGA AAAATCTCGT GCTGTTCGTC AAGCAAAAGA TGAGCGTACT 
TTTCATATCT TTTACCAATT GTTATCTGGA GCAGGAGAAC ATCTGAAGTC TGATTTGCTC 
CTTGAAGGAT TTAATAACTA CAGATTTCTC TCCAATGGCT ATATTCCTAT TCCGGGACAA 
CAAGACAAAG ATAACTTCCA GGAGACAATG GAGGCAATGC ATATAATGGG CTTCTCCCAT 
GAAGAGATTC TGTCAATGCT TAAAGTGGTA TCTTCAGTGC TTCAGTTTGG AAATATTTCT 
TTCAAAAAGG AGCGAAACAC TGATCAAGCC TCCATGCCGG AAAATACAGT TGCACAGAAG 
CTCTGCCATC TTCTTGGCAT GAATGTGATG GAGTTCACTC GGGCCATTCT CACCCCCCGG 
ATCAAGGTCG GCCGAGACTA CGTGCAGAAA GCCCAGACCA AAGAACAAGC AGATTTTGCA 
GTAGAAGCTT TGGCAAAAGC TACCTATGAG CGGCTCTTCC GCTGGCTCGT TCACCGCATC 
AATAAAGCTC TGGACAGGAC CAAACGGCAA GGGGCCTCCT TCATTGGGAT CCTGGACATC 
GCTGGATTTG AGATTTTTGA GCTGAACTCA TTCGAGCAAC TGTGCATCAA CTACACCAAC 
GAGAAGCTGC AGCAGCTCTT CAACCACACC ATGTTCATCC TGGAGCAGGA GGAGTACCAG 
CGCGAGGGCA TCGAGTGGAA CTTCATCGAC TTTGGCCTCG ACCTGCAGCC CTGCATTGAC 
CTCATCGAGA GACCTGCTAA CCCTCCTGGC GTGCTGGCCC TTCTGGATGA AGAGTGCTGG 
TTCCCTAAAG CCACAGATAA GACCTTTGTT GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC 
TCCAAGTTTC AGAAACCACG TCAATTGAAA GACAAAGCTG ATTTTTGCAT TATCCATTAT 
GCAGGGAAGG TGGACTACAA GGCAGACGAG TGGCTGATGA AGAACATGGA CCCCCTGAAT 
GACAACGTGG CCACCCTCCT GCACCAGTCC TCTGACAGAT TCGTGGCAGA GCTCTGGAAG 
GATGAGATTC AGAATATTCA GAGAGCTTCT TTCTATGACA GTGTTTCTGG TCTTCATGAG 
CCACCAGTGG ACCGGATCGT GGGTCTGGAT CAGGTCACGG GGATGACGGA GACGGCATTC 
GGCTCCGCCT ATAAAACCAA GAAGGGCATG TTCCGCACTG TCGGGCAGCT CTACAAAGAA 
TCGCTCACCA AGCTGATGGC GACGCTCCGG AACACCAACC CGAACTTCGT GCGCTGCATC 
ATCCCAAATC ACGAGAAGCG GGCTGGGAAA CTGGACCCAC ACCTGGTGCT GGATCAGCTC 
CGCTGCAATG GCGTCCTGGA AGGGATCCGC ATCTGCCGCC AGGGCTTCCC CAACCGGATC 
GTTTTCCAGG AATTCAGACA GAGATACGAG ATCCTCACTC CAAATGCTAT CCCTAAGGGT 
TTCATGGATG GCAAACAGGC TTGTGAACGA ATGATCCGGG CTTTAGAATT GGACCCAAAC 
TTGTACAGAA TTGGACAGAG CAAGATATTT TTCAGAGCTG GAGTCTTGGC ACACTTAGAG 
GAAGAACGAG ATTTAAAAAT TACCGACATC ATTATCTTCT TCCAGGCTGT TTGCAGGGGT 
TATTTAGCCA GAAAGGCCTT CGCCAAGAAG CAGCAGCAGC TGAGTGCCCT GAAGGTGCTG 
CAGCGCAACT GTGCCGCGTA CCTGAAGCTG CGGCACTGGC AGTGGTGGCG CGTCTTCACC 
AAGGTGAAAC CACTCCTACA AGTCACTCGT CAAGAGGAGG AGCTCCAGGC CAAAGATGAA 
GAGCTGCTGA AGGTGAAAGA GAAGCAGACG AAGGTGGAAG GGGAGCTGGA GGAGATGGAG 
AGGAAGCACC AACAGCTGCT GGAGGAGAAG AATATCCTGG CAGAGCAGCT GCAGGCGGAG 
ACCGAGCTCT TTGCCGAAGC AGAGGAGATG AGGGCCAGAC TTGCCGCTAA GAAGCAGGAA 
TTAGAAGAGA TTCTCCATGA CTTGGAATCT AGGGTAGAAG AAGAAGAAGA AAGAAACCAA 
ATCCTCCAAA ATGAAAAGAA GAAAATGCAA GCACACATTC AGGACCTGGA GGAGCAGCTA 
GATGAGGAGG AGGGGGCTCG CCAGAAGCTG CAGCTGGAGA AGGTGACGGC GGAGGCCAAG 
ATCAAGAAGA TGGAAGAAGA GATTCTGCTT CTGGAGGACC AGAACTCCAA GTTTATCAAA 
GAAAAGAAAC TCATGGAAGA TCGCATTGCA GAATGTTCCT CTCAGCTGGC TGAAGAGGAA 
GAAAAGGCAA AAAACTTGGC CAAAATCAGG AATAAGCAAG AAGTAATGAT CTCGGATTTA 
GAAGAGCGCT TAAAGAAGGA GGAGAAGACG CGCCAGGAGC TGGAGAAGGC CAAAAGGAAA 
CTGGACGGGG AGACCACGGA CCTGCAAGAC CAGATCGCCG AGCTCCAGGC GCAGATTGAC 
GAGCTTAAGA TCCAGGTGGC CAAGAAAGAG GAGGAGCTGC AGGGCGCCCT GGCCAGGGGT 
GACGACGAGA CGCTACACAA GAACAACGCA CTAAAAGTCG TGCGAGAGCT GCAGGCCCAG 
ATCGCCGAGC TGCAGGAGGA CTTTGAATCT GAGAAGGCCT CGCGGAACAA AGCAGAGAAG 
CAGAAGAGGG ACTTGAGTGA GGAGCTGGAG GCACTCAAGA CGGAGCTGGA GGACACGCTG 
GACACCACGG CGGCCCAGCA GGAACTGCGT ACAAAACGAG AACAAGAAGT GGCGGAGCTG 
AAGAAGGCTC TCGAGGAGGA AACGAAGAGC CACGAGGCTC AGATCCAGGA CATGAGGCAG 
AGGCATGCGA CCGCCTTGGA GGAGCTGTCG GAGCAGCTGG AGCAGGCCAA GAGGTTCAAA 
GCAAATCTGG AAAAGAACAA GCAGGGCCTG GAGACAGACA ACAAGGAGCT GGCGTGCGAG 
GTCAAGGTGC TGCAGCAGGT CAAGGCTGAG TCCGAGCACA AGAGGAAGAA GCTGGACGCC 
CAGGTCCAGG AGCTCCACGC CAAGGTCTCA GAGGGAGACA GACTCAGGGT GGAGCTGGCA 
GAGAAAGCCA ATAAGTTACA GAATGAATTG GATAATGTCT CAACTCTTCT AGAAGAAGCA 
GAGAAGAAGG GTATTAAATT TGCTAAGGAT GCAGCTGGTC TGGAGTCTCA GCTCCAAGAC 
ACACAGGAGC TTCTTCAGGA GGAGACGCGC CAGAAACTGA ACCTGAGCAG TCGGATCCGG 
CAGCTGGAGG AGGAGAGGAG CAGCCTCCAG GAGCAGCAGG AGGAAGAGGA GGAGGCCAGG 
AGGAGCCTGG AGAAGCAGCT GCAGGCCCTG CAGGCCCAGT TGACGGATAC CAAGAAGAAA 
GTAGATGATG ACCTGGGGAC AATTGAAAAT TTGGAAGAAG CCAAGAAGAA GCTCCTCAAG 
GATGTGGAGG TGCTGAGCCA GCGCCTGGAG GAGAAGGCCC TGGCCTATGA CAAGCTGGAG 
AAGACCAAGA CCCGCCTGCA GCAGGAGCTG GATGACCTGC TGGTAGACCT GGACCACCAG 
CGCCAGATCG TCTCCAACTT GGAGAAGAAG CAGAAGAAGT TTGATCAGCT GTTAGCGGAA 
GAGAAGAACA TCTCTGCCCG CTACGCAGAA GAGCGCGACC GGGCAGAGGC AGAGGCCCGA 
GAGAAGGAGA CCAAAGCCCT GTCCCTGGCC CGCGCCCTGG AGGAGGCCCT GGAGGCCAGG 
GAGGAGGCTG AGCGACAGAA TAAGCAGCTG CGGGCGGACA TGGAGGACCT CATGAGCTCC 
AAGGACGATG TGGGCAAGAA CGTTCATGAA CTTGAGAAAT CCAAACGGGC CCTAGAGCAG 
CAGGTGGAGG AAATGAGGAC CCAACTGGAG GAGCTGGAAG ATGAGCTCCA GGCTACAGAA 
GACGCCAAGC TTCGCCTAGA GGTCAACATG CAAGCCATGA AAGCCCAGTT TGAGAGAGAC 
TTGCAGACCA GGGATGAGCA GAATGAGGAA AAGAAGCGGC TGCTCATTAA ACAGGTGCGG 
GAGCTTGAGG CAGAGCTGGA GGATGAGCGG AAACAGCGGG CCCTGGCTGT TGCCTCGAAG 
AAGAAAATGG AAATAGACCT GAAGGACCTG GAGGCTCAAA TCGAGGCAGC TAACAAAGCA 
AGGGATGAAG TGATCAAGCA GCTCCGCAAA CTCCAGGCTC AGATGAAGGA CTACCAGCGT 
GAATTAGAAG AAGCTCGTGC ATCCCGGGAT GAGATTTTTG CTCAATCCAA AGAGAGTGAG 
AAGAAACTGA AAAGCCTGGA AGCAGAAATC CTTCAGTTGC AGGAGGAACT TGCCTCATCC 
GAGCGAGCCC GCCGACATGC TGAGCAGGAG AGAGACGAGC TGGCGGACGA GATTGCCAAT 
AGCGCCTCCG GCAAGTCTGC ACTCCTGGAT GAGAAGCGGC GTCTGGAGGC TCGGATCGCA 
CAGCTAGAGG AGGAGCTGGA GGAGGAGCAG AGCAACATGG AGCTGCTCAA TGACCGCTTC 
CGCAAGACCA CACTGCAGGT GGACACGCTG AACACTGAGC TGGCGGCCGA GCGCAGCGCT 
GCCCAGAAGA GCGATAATGC GCGCCAGCAG CTGGAGCGGC AGAACAAGGA GTTGAAGGCC 
AAGCTGCAGG AGCTGGAAGG CGCTGTCAAG TCCAAGTTCA AGGCCACCAT CTCTGCTCTA 
GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG CTTGAGCAGG AAGCCAAGGA AAGAGCCGCT 
GCCAATAAAC TAGTCCGTCG TACCGAGAAG AAGCTGAAAG AAATCTTCAT GCAGGTTGAA 
GATGAACGCC GCCATGCTGA CCAGTATAAA GAACAGATGG AGAAGGCCAA TGCCAGGATG 
AAGCAGCTCA AGCGGCAGCT GGAGGAAGCA GAGGAAGAAG CTACACGTGC CAACGCATCT 
CGGCGCAAAC TCCAGCGGGA ACTGGATGAT GCCACTGAGG CCAACGAGGG CCTGAGCCGC 
GAGGTCAGCA CCCTGAAGAA CCGGCTGAGG CGAGGCGGCC CGATCAGCTT CTCCTCCAGC 
CGATCAGGCC GGCGCCAGCT GCACATTGAG GGCGCGTCCC TGGAGCTGTC GGACGACGAC 
ACAGAAAGTA AGACCAGTGA CATCAACGAG ACACAGCCGC CGCAGTCCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_015458671.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314157.1 Bos taurus myosin heavy chain 10 (MYH10), transcript variant X4, mRNA.

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CloneID	OBa226017
Clone ID Related Accession (Same CDS sequence)	XM_015458671.2
Accession Version	XM_015458671.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myosin-10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458671.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTCAGGAAT CACCTAAACC AGTGAAACAC 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC ATTTGGAAAC 
GCAAAGACTG TGAAAAATGA TAACTCATCT CGTTTTGGCA AGTTTATTCG GATCAACTTT 
GATGTAACTG GCTATATCGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAATT GTTATCTGGA 
GCAGGAGAAC ATCTGAAGTC TGATTTGCTC CTTGAAGGAT TTAATAACTA CAGATTTCTC 
TCCAATGGCT ATATTCCTAT TCCGGGACAA CAAGACAAAG ATAACTTCCA GGAGACAATG 
GAGGCAATGC ATATAATGGG CTTCTCCCAT GAAGAGATTC TGTCAATGCT TAAAGTGGTA 
TCTTCAGTGC TTCAGTTTGG AAATATTTCT TTCAAAAAGG AGCGAAACAC TGATCAAGCC 
TCCATGCCGG AAAATACAGT TGCACAGAAG CTCTGCCATC TTCTTGGCAT GAATGTGATG 
GAGTTCACTC GGGCCATTCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA CGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCA GTAGAAGCTT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GCTGGCTCGT TCACCGCATC AATAAAGCTC TGGACAGGAC CAAACGGCAA 
GGGGCCTCCT TCATTGGGAT CCTGGACATC GCTGGATTTG AGATTTTTGA GCTGAACTCA 
TTCGAGCAAC TGTGCATCAA CTACACCAAC GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAA CTTCATCGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATTGAC CTCATCGAGA GACCTGCTAA CCCTCCTGGC 
GTGCTGGCCC TTCTGGATGA AGAGTGCTGG TTCCCTAAAG CCACAGATAA GACCTTTGTT 
GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC TCCAAGTTTC AGAAACCACG TCAATTGAAA 
GACAAAGCTG ATTTTTGCAT TATCCATTAT GCAGGGAAGG TGGACTACAA GGCAGACGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAT GACAACGTGG CCACCCTCCT GCACCAGTCC 
TCTGACAGAT TCGTGGCAGA GCTCTGGAAG GATGTGGACC GGATCGTGGG TCTGGATCAG 
GTCACGGGGA TGACGGAGAC GGCATTCGGC TCCGCCTATA AAACCAAGAA GGGCATGTTC 
CGCACTGTCG GGCAGCTCTA CAAAGAATCG CTCACCAAGC TGATGGCGAC GCTCCGGAAC 
ACCAACCCGA ACTTCGTGCG CTGCATCATC CCAAATCACG AGAAGCGGGC TGGGAAACTG 
GACCCACACC TGGTGCTGGA TCAGCTCCGC TGCAATGGCG TCCTGGAAGG GATCCGCATC 
TGCCGCCAGG GCTTCCCCAA CCGGATCGTT TTCCAGGAAT TCAGACAGAG ATACGAGATC 
CTCACTCCAA ATGCTATCCC TAAGGGTTTC ATGGATGGCA AACAGGCTTG TGAACGAATG 
ATCCGGGCTT TAGAATTGGA CCCAAACTTG TACAGAATTG GACAGAGCAA GATATTTTTC 
AGAGCTGGAG TCTTGGCACA CTTAGAGGAA GAACGAGATT TAAAAATTAC CGACATCATT 
ATCTTCTTCC AGGCTGTTTG CAGGGGTTAT TTAGCCAGAA AGGCCTTCGC CAAGAAGCAG 
CAGCAGCTGA GTGCCCTGAA GGTGCTGCAG CGCAACTGTG CCGCGTACCT GAAGCTGCGG 
CACTGGCAGT GGTGGCGCGT CTTCACCAAG GTGAAACCAC TCCTACAAGT CACTCGTCAA 
GAGGAGGAGC TCCAGGCCAA AGATGAAGAG CTGCTGAAGG TGAAAGAGAA GCAGACGAAG 
GTGGAAGGGG AGCTGGAGGA GATGGAGAGG AAGCACCAAC AGCTGCTGGA GGAGAAGAAT 
ATCCTGGCAG AGCAGCTGCA GGCGGAGACC GAGCTCTTTG CCGAAGCAGA GGAGATGAGG 
GCCAGACTTG CCGCTAAGAA GCAGGAATTA GAAGAGATTC TCCATGACTT GGAATCTAGG 
GTAGAAGAAG AAGAAGAAAG AAACCAAATC CTCCAAAATG AAAAGAAGAA AATGCAAGCA 
CACATTCAGG ACCTGGAGGA GCAGCTAGAT GAGGAGGAGG GGGCTCGCCA GAAGCTGCAG 
CTGGAGAAGG TGACGGCGGA GGCCAAGATC AAGAAGATGG AAGAAGAGAT TCTGCTTCTG 
GAGGACCAGA ACTCCAAGTT TATCAAAGAA AAGAAACTCA TGGAAGATCG CATTGCAGAA 
TGTTCCTCTC AGCTGGCTGA AGAGGAAGAA AAGGCAAAAA ACTTGGCCAA AATCAGGAAT 
AAGCAAGAAG TAATGATCTC GGATTTAGAA GAGCGCTTAA AGAAGGAGGA GAAGACGCGC 
CAGGAGCTGG AGAAGGCCAA AAGGAAACTG GACGGGGAGA CCACGGACCT GCAAGACCAG 
ATCGCCGAGC TCCAGGCGCA GATTGACGAG CTTAAGATCC AGGTGGCCAA GAAAGAGGAG 
GAGCTGCAGG GCGCCCTGGC CAGGGGTGAC GACGAGACGC TACACAAGAA CAACGCACTA 
AAAGTCGTGC GAGAGCTGCA GGCCCAGATC GCCGAGCTGC AGGAGGACTT TGAATCTGAG 
AAGGCCTCGC GGAACAAAGC AGAGAAGCAG AAGAGGGACT TGAGTGAGGA GCTGGAGGCA 
CTCAAGACGG AGCTGGAGGA CACGCTGGAC ACCACGGCGG CCCAGCAGGA ACTGCGTACA 
AAACGAGAAC AAGAAGTGGC GGAGCTGAAG AAGGCTCTCG AGGAGGAAAC GAAGAGCCAC 
GAGGCTCAGA TCCAGGACAT GAGGCAGAGG CATGCGACCG CCTTGGAGGA GCTGTCGGAG 
CAGCTGGAGC AGGCCAAGAG GTTCAAAGCA AATCTGGAAA AGAACAAGCA GGGCCTGGAG 
ACAGACAACA AGGAGCTGGC GTGCGAGGTC AAGGTGCTGC AGCAGGTCAA GGCTGAGTCC 
GAGCACAAGA GGAAGAAGCT GGACGCCCAG GTCCAGGAGC TCCACGCCAA GGTCTCAGAG 
GGAGACAGAC TCAGGGTGGA GCTGGCAGAG AAAGCCAATA AGTTACAGAA TGAATTGGAT 
AATGTCTCAA CTCTTCTAGA AGAAGCAGAG AAGAAGGGTA TTAAATTTGC TAAGGATGCA 
GCTGGTCTGG AGTCTCAGCT CCAAGACACA CAGGAGCTTC TTCAGGAGGA GACGCGCCAG 
AAACTGAACC TGAGCAGTCG GATCCGGCAG CTGGAGGAGG AGAGGAGCAG CCTCCAGGAG 
CAGCAGGAGG AAGAGGAGGA GGCCAGGAGG AGCCTGGAGA AGCAGCTGCA GGCCCTGCAG 
GCCCAGTTGA CGGATACCAA GAAGAAAGTA GATGATGACC TGGGGACAAT TGAAAATTTG 
GAAGAAGCCA AGAAGAAGCT CCTCAAGGAT GTGGAGGTGC TGAGCCAGCG CCTGGAGGAG 
AAGGCCCTGG CCTATGACAA GCTGGAGAAG ACCAAGACCC GCCTGCAGCA GGAGCTGGAT 
GACCTGCTGG TAGACCTGGA CCACCAGCGC CAGATCGTCT CCAACTTGGA GAAGAAGCAG 
AAGAAGTTTG ATCAGCTGTT AGCGGAAGAG AAGAACATCT CTGCCCGCTA CGCAGAAGAG 
CGCGACCGGG CAGAGGCAGA GGCCCGAGAG AAGGAGACCA AAGCCCTGTC CCTGGCCCGC 
GCCCTGGAGG AGGCCCTGGA GGCCAGGGAG GAGGCTGAGC GACAGAATAA GCAGCTGCGG 
GCGGACATGG AGGACCTCAT GAGCTCCAAG GACGATGTGG GCAAGAACGT TCATGAACTT 
GAGAAATCCA AACGGGCCCT AGAGCAGCAG GTGGAGGAAA TGAGGACCCA ACTGGAGGAG 
CTGGAAGATG AGCTCCAGGC TACAGAAGAC GCCAAGCTTC GCCTAGAGGT CAACATGCAA 
GCCATGAAAG CCCAGTTTGA GAGAGACTTG CAGACCAGGG ATGAGCAGAA TGAGGAAAAG 
AAGCGGCTGC TCATTAAACA GGTGCGGGAG CTTGAGGCAG AGCTGGAGGA TGAGCGGAAA 
CAGCGGGCCC TGGCTGTTGC CTCGAAGAAG AAAATGGAAA TAGACCTGAA GGACCTGGAG 
GCTCAAATCG AGGCAGCTAA CAAAGCAAGG GATGAAGTGA TCAAGCAGCT CCGCAAACTC 
CAGGCTCAGA TGAAGGACTA CCAGCGTGAA TTAGAAGAAG CTCGTGCATC CCGGGATGAG 
ATTTTTGCTC AATCCAAAGA GAGTGAGAAG AAACTGAAAA GCCTGGAAGC AGAAATCCTT 
CAGTTGCAGG AGGAACTTGC CTCATCCGAG CGAGCCCGCC GACATGCTGA GCAGGAGAGA 
GACGAGCTGG CGGACGAGAT TGCCAATAGC GCCTCCGGCA AGTCTGCACT CCTGGATGAG 
AAGCGGCGTC TGGAGGCTCG GATCGCACAG CTAGAGGAGG AGCTGGAGGA GGAGCAGAGC 
AACATGGAGC TGCTCAATGA CCGCTTCCGC AAGACCACAC TGCAGGTGGA CACGCTGAAC 
ACTGAGCTGG CGGCCGAGCG CAGCGCTGCC CAGAAGAGCG ATAATGCGCG CCAGCAGCTG 
GAGCGGCAGA ACAAGGAGTT GAAGGCCAAG CTGCAGGAGC TGGAAGGCGC TGTCAAGTCC 
AAGTTCAAGG CCACCATCTC TGCTCTAGAA GCCAAGATTG GGCAGCTGGA GGAGCAGCTT 
GAGCAGGAAG CCAAGGAAAG AGCCGCTGCC AATAAACTAG TCCGTCGTAC CGAGAAGAAG 
CTGAAAGAAA TCTTCATGCA GGTTGAAGAT GAACGCCGCC ATGCTGACCA GTATAAAGAA 
CAGATGGAGA AGGCCAATGC CAGGATGAAG CAGCTCAAGC GGCAGCTGGA GGAAGCAGAG 
GAAGAAGCTA CACGTGCCAA CGCATCTCGG CGCAAACTCC AGCGGGAACT GGATGATGCC 
ACTGAGGCCA ACGAGGGCCT GAGCCGCGAG GTCAGCACCC TGAAGAACCG GCTGAGGCGA 
GGCGGCCCGA TCAGCTTCTC CTCCAGCCGA TCAGGCCGGC GCCAGCTGCA CATTGAGGGC 
GCGTCCCTGG AGCTGTCGGA CGACGACACA GAAAGTAAGA CCAGTGACAT CAACGAGACA 
CAGCCGCCGC AGTCCGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314157.1
CDS	1..5961
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVLVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPQESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKEKQTKVEGELEEMERKHQQLLEEKNILAEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKIQVAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETKSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSTLLEEAEKKGIKFAKDAAGLESQLQDTQELLQEETRQKLNLSSRIRQLEEERSSLQEQQEEEEEARRSLEKQLQALQAQLTDTKKKVDDDLGTIENLEEAKKKLLKDVEVLSQRLEEKALAYDKLEKTKTRLQQELDDLLVDLDHQRQIVSNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALEAREEAERQNKQLRADMEDLMSSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLIKQVRELEAELEDERKQRALAVASKKKMEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNTELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDINETQPPQSE

Target ORF information:

RefSeq Version	XM_015458671.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin heavy chain 10 (MYH10), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458671.2

ORF Insert Sequence:

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTCAGGAAT CACCTAAACC AGTGAAACAC 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC ATTTGGAAAC 
GCAAAGACTG TGAAAAATGA TAACTCATCT CGTTTTGGCA AGTTTATTCG GATCAACTTT 
GATGTAACTG GCTATATCGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAATT GTTATCTGGA 
GCAGGAGAAC ATCTGAAGTC TGATTTGCTC CTTGAAGGAT TTAATAACTA CAGATTTCTC 
TCCAATGGCT ATATTCCTAT TCCGGGACAA CAAGACAAAG ATAACTTCCA GGAGACAATG 
GAGGCAATGC ATATAATGGG CTTCTCCCAT GAAGAGATTC TGTCAATGCT TAAAGTGGTA 
TCTTCAGTGC TTCAGTTTGG AAATATTTCT TTCAAAAAGG AGCGAAACAC TGATCAAGCC 
TCCATGCCGG AAAATACAGT TGCACAGAAG CTCTGCCATC TTCTTGGCAT GAATGTGATG 
GAGTTCACTC GGGCCATTCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA CGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCA GTAGAAGCTT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GCTGGCTCGT TCACCGCATC AATAAAGCTC TGGACAGGAC CAAACGGCAA 
GGGGCCTCCT TCATTGGGAT CCTGGACATC GCTGGATTTG AGATTTTTGA GCTGAACTCA 
TTCGAGCAAC TGTGCATCAA CTACACCAAC GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAA CTTCATCGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATTGAC CTCATCGAGA GACCTGCTAA CCCTCCTGGC 
GTGCTGGCCC TTCTGGATGA AGAGTGCTGG TTCCCTAAAG CCACAGATAA GACCTTTGTT 
GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC TCCAAGTTTC AGAAACCACG TCAATTGAAA 
GACAAAGCTG ATTTTTGCAT TATCCATTAT GCAGGGAAGG TGGACTACAA GGCAGACGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAT GACAACGTGG CCACCCTCCT GCACCAGTCC 
TCTGACAGAT TCGTGGCAGA GCTCTGGAAG GATGTGGACC GGATCGTGGG TCTGGATCAG 
GTCACGGGGA TGACGGAGAC GGCATTCGGC TCCGCCTATA AAACCAAGAA GGGCATGTTC 
CGCACTGTCG GGCAGCTCTA CAAAGAATCG CTCACCAAGC TGATGGCGAC GCTCCGGAAC 
ACCAACCCGA ACTTCGTGCG CTGCATCATC CCAAATCACG AGAAGCGGGC TGGGAAACTG 
GACCCACACC TGGTGCTGGA TCAGCTCCGC TGCAATGGCG TCCTGGAAGG GATCCGCATC 
TGCCGCCAGG GCTTCCCCAA CCGGATCGTT TTCCAGGAAT TCAGACAGAG ATACGAGATC 
CTCACTCCAA ATGCTATCCC TAAGGGTTTC ATGGATGGCA AACAGGCTTG TGAACGAATG 
ATCCGGGCTT TAGAATTGGA CCCAAACTTG TACAGAATTG GACAGAGCAA GATATTTTTC 
AGAGCTGGAG TCTTGGCACA CTTAGAGGAA GAACGAGATT TAAAAATTAC CGACATCATT 
ATCTTCTTCC AGGCTGTTTG CAGGGGTTAT TTAGCCAGAA AGGCCTTCGC CAAGAAGCAG 
CAGCAGCTGA GTGCCCTGAA GGTGCTGCAG CGCAACTGTG CCGCGTACCT GAAGCTGCGG 
CACTGGCAGT GGTGGCGCGT CTTCACCAAG GTGAAACCAC TCCTACAAGT CACTCGTCAA 
GAGGAGGAGC TCCAGGCCAA AGATGAAGAG CTGCTGAAGG TGAAAGAGAA GCAGACGAAG 
GTGGAAGGGG AGCTGGAGGA GATGGAGAGG AAGCACCAAC AGCTGCTGGA GGAGAAGAAT 
ATCCTGGCAG AGCAGCTGCA GGCGGAGACC GAGCTCTTTG CCGAAGCAGA GGAGATGAGG 
GCCAGACTTG CCGCTAAGAA GCAGGAATTA GAAGAGATTC TCCATGACTT GGAATCTAGG 
GTAGAAGAAG AAGAAGAAAG AAACCAAATC CTCCAAAATG AAAAGAAGAA AATGCAAGCA 
CACATTCAGG ACCTGGAGGA GCAGCTAGAT GAGGAGGAGG GGGCTCGCCA GAAGCTGCAG 
CTGGAGAAGG TGACGGCGGA GGCCAAGATC AAGAAGATGG AAGAAGAGAT TCTGCTTCTG 
GAGGACCAGA ACTCCAAGTT TATCAAAGAA AAGAAACTCA TGGAAGATCG CATTGCAGAA 
TGTTCCTCTC AGCTGGCTGA AGAGGAAGAA AAGGCAAAAA ACTTGGCCAA AATCAGGAAT 
AAGCAAGAAG TAATGATCTC GGATTTAGAA GAGCGCTTAA AGAAGGAGGA GAAGACGCGC 
CAGGAGCTGG AGAAGGCCAA AAGGAAACTG GACGGGGAGA CCACGGACCT GCAAGACCAG 
ATCGCCGAGC TCCAGGCGCA GATTGACGAG CTTAAGATCC AGGTGGCCAA GAAAGAGGAG 
GAGCTGCAGG GCGCCCTGGC CAGGGGTGAC GACGAGACGC TACACAAGAA CAACGCACTA 
AAAGTCGTGC GAGAGCTGCA GGCCCAGATC GCCGAGCTGC AGGAGGACTT TGAATCTGAG 
AAGGCCTCGC GGAACAAAGC AGAGAAGCAG AAGAGGGACT TGAGTGAGGA GCTGGAGGCA 
CTCAAGACGG AGCTGGAGGA CACGCTGGAC ACCACGGCGG CCCAGCAGGA ACTGCGTACA 
AAACGAGAAC AAGAAGTGGC GGAGCTGAAG AAGGCTCTCG AGGAGGAAAC GAAGAGCCAC 
GAGGCTCAGA TCCAGGACAT GAGGCAGAGG CATGCGACCG CCTTGGAGGA GCTGTCGGAG 
CAGCTGGAGC AGGCCAAGAG GTTCAAAGCA AATCTGGAAA AGAACAAGCA GGGCCTGGAG 
ACAGACAACA AGGAGCTGGC GTGCGAGGTC AAGGTGCTGC AGCAGGTCAA GGCTGAGTCC 
GAGCACAAGA GGAAGAAGCT GGACGCCCAG GTCCAGGAGC TCCACGCCAA GGTCTCAGAG 
GGAGACAGAC TCAGGGTGGA GCTGGCAGAG AAAGCCAATA AGTTACAGAA TGAATTGGAT 
AATGTCTCAA CTCTTCTAGA AGAAGCAGAG AAGAAGGGTA TTAAATTTGC TAAGGATGCA 
GCTGGTCTGG AGTCTCAGCT CCAAGACACA CAGGAGCTTC TTCAGGAGGA GACGCGCCAG 
AAACTGAACC TGAGCAGTCG GATCCGGCAG CTGGAGGAGG AGAGGAGCAG CCTCCAGGAG 
CAGCAGGAGG AAGAGGAGGA GGCCAGGAGG AGCCTGGAGA AGCAGCTGCA GGCCCTGCAG 
GCCCAGTTGA CGGATACCAA GAAGAAAGTA GATGATGACC TGGGGACAAT TGAAAATTTG 
GAAGAAGCCA AGAAGAAGCT CCTCAAGGAT GTGGAGGTGC TGAGCCAGCG CCTGGAGGAG 
AAGGCCCTGG CCTATGACAA GCTGGAGAAG ACCAAGACCC GCCTGCAGCA GGAGCTGGAT 
GACCTGCTGG TAGACCTGGA CCACCAGCGC CAGATCGTCT CCAACTTGGA GAAGAAGCAG 
AAGAAGTTTG ATCAGCTGTT AGCGGAAGAG AAGAACATCT CTGCCCGCTA CGCAGAAGAG 
CGCGACCGGG CAGAGGCAGA GGCCCGAGAG AAGGAGACCA AAGCCCTGTC CCTGGCCCGC 
GCCCTGGAGG AGGCCCTGGA GGCCAGGGAG GAGGCTGAGC GACAGAATAA GCAGCTGCGG 
GCGGACATGG AGGACCTCAT GAGCTCCAAG GACGATGTGG GCAAGAACGT TCATGAACTT 
GAGAAATCCA AACGGGCCCT AGAGCAGCAG GTGGAGGAAA TGAGGACCCA ACTGGAGGAG 
CTGGAAGATG AGCTCCAGGC TACAGAAGAC GCCAAGCTTC GCCTAGAGGT CAACATGCAA 
GCCATGAAAG CCCAGTTTGA GAGAGACTTG CAGACCAGGG ATGAGCAGAA TGAGGAAAAG 
AAGCGGCTGC TCATTAAACA GGTGCGGGAG CTTGAGGCAG AGCTGGAGGA TGAGCGGAAA 
CAGCGGGCCC TGGCTGTTGC CTCGAAGAAG AAAATGGAAA TAGACCTGAA GGACCTGGAG 
GCTCAAATCG AGGCAGCTAA CAAAGCAAGG GATGAAGTGA TCAAGCAGCT CCGCAAACTC 
CAGGCTCAGA TGAAGGACTA CCAGCGTGAA TTAGAAGAAG CTCGTGCATC CCGGGATGAG 
ATTTTTGCTC AATCCAAAGA GAGTGAGAAG AAACTGAAAA GCCTGGAAGC AGAAATCCTT 
CAGTTGCAGG AGGAACTTGC CTCATCCGAG CGAGCCCGCC GACATGCTGA GCAGGAGAGA 
GACGAGCTGG CGGACGAGAT TGCCAATAGC GCCTCCGGCA AGTCTGCACT CCTGGATGAG 
AAGCGGCGTC TGGAGGCTCG GATCGCACAG CTAGAGGAGG AGCTGGAGGA GGAGCAGAGC 
AACATGGAGC TGCTCAATGA CCGCTTCCGC AAGACCACAC TGCAGGTGGA CACGCTGAAC 
ACTGAGCTGG CGGCCGAGCG CAGCGCTGCC CAGAAGAGCG ATAATGCGCG CCAGCAGCTG 
GAGCGGCAGA ACAAGGAGTT GAAGGCCAAG CTGCAGGAGC TGGAAGGCGC TGTCAAGTCC 
AAGTTCAAGG CCACCATCTC TGCTCTAGAA GCCAAGATTG GGCAGCTGGA GGAGCAGCTT 
GAGCAGGAAG CCAAGGAAAG AGCCGCTGCC AATAAACTAG TCCGTCGTAC CGAGAAGAAG 
CTGAAAGAAA TCTTCATGCA GGTTGAAGAT GAACGCCGCC ATGCTGACCA GTATAAAGAA 
CAGATGGAGA AGGCCAATGC CAGGATGAAG CAGCTCAAGC GGCAGCTGGA GGAAGCAGAG 
GAAGAAGCTA CACGTGCCAA CGCATCTCGG CGCAAACTCC AGCGGGAACT GGATGATGCC 
ACTGAGGCCA ACGAGGGCCT GAGCCGCGAG GTCAGCACCC TGAAGAACCG GCTGAGGCGA 
GGCGGCCCGA TCAGCTTCTC CTCCAGCCGA TCAGGCCGGC GCCAGCTGCA CATTGAGGGC 
GCGTCCCTGG AGCTGTCGGA CGACGACACA GAAAGTAAGA CCAGTGACAT CAACGAGACA 
CAGCCGCCGC AGTCCGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_005220298.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220355.1 Bos taurus myosin heavy chain 10 (MYH10), transcript variant X2, mRNA.

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CloneID	OBa226015
Clone ID Related Accession (Same CDS sequence)	XM_005220298.4 , XM_005220297.4
Accession Version	XM_005220298.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myosin-10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220298.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTCAGGAAT CACCTAAACC AGTGAAACAC 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC ATTTGGAAAC 
GCAAAGACTG TGAAAAATGA TAACTCATCT CGTTTTGGCA AGTTTATTCG GATCAACTTT 
GATGTAACTG GCTATATCGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAATT GTTATCTGGA 
GCAGGAGAAC ATCTGAAGTC TGATTTGCTC CTTGAAGGAT TTAATAACTA CAGATTTCTC 
TCCAATGGCT ATATTCCTAT TCCGGGACAA CAAGACAAAG ATAACTTCCA GGAGACAATG 
GAGGCAATGC ATATAATGGG CTTCTCCCAT GAAGAGATTC TGTCAATGCT TAAAGTGGTA 
TCTTCAGTGC TTCAGTTTGG AAATATTTCT TTCAAAAAGG AGCGAAACAC TGATCAAGCC 
TCCATGCCGG AAAATACAGT TGCACAGAAG CTCTGCCATC TTCTTGGCAT GAATGTGATG 
GAGTTCACTC GGGCCATTCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA CGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCA GTAGAAGCTT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GCTGGCTCGT TCACCGCATC AATAAAGCTC TGGACAGGAC CAAACGGCAA 
GGGGCCTCCT TCATTGGGAT CCTGGACATC GCTGGATTTG AGATTTTTGA GCTGAACTCA 
TTCGAGCAAC TGTGCATCAA CTACACCAAC GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAA CTTCATCGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATTGAC CTCATCGAGA GACCTGCTAA CCCTCCTGGC 
GTGCTGGCCC TTCTGGATGA AGAGTGCTGG TTCCCTAAAG CCACAGATAA GACCTTTGTT 
GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC TCCAAGTTTC AGAAACCACG TCAATTGAAA 
GACAAAGCTG ATTTTTGCAT TATCCATTAT GCAGGGAAGG TGGACTACAA GGCAGACGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAT GACAACGTGG CCACCCTCCT GCACCAGTCC 
TCTGACAGAT TCGTGGCAGA GCTCTGGAAG GATGAGATTC AGAATATTCA GAGAGCTTCT 
TTCTATGACA GTGTTTCTGG TCTTCATGAG CCACCAGTGG ACCGGATCGT GGGTCTGGAT 
CAGGTCACGG GGATGACGGA GACGGCATTC GGCTCCGCCT ATAAAACCAA GAAGGGCATG 
TTCCGCACTG TCGGGCAGCT CTACAAAGAA TCGCTCACCA AGCTGATGGC GACGCTCCGG 
AACACCAACC CGAACTTCGT GCGCTGCATC ATCCCAAATC ACGAGAAGCG GGCTGGGAAA 
CTGGACCCAC ACCTGGTGCT GGATCAGCTC CGCTGCAATG GCGTCCTGGA AGGGATCCGC 
ATCTGCCGCC AGGGCTTCCC CAACCGGATC GTTTTCCAGG AATTCAGACA GAGATACGAG 
ATCCTCACTC CAAATGCTAT CCCTAAGGGT TTCATGGATG GCAAACAGGC TTGTGAACGA 
ATGATCCGGG CTTTAGAATT GGACCCAAAC TTGTACAGAA TTGGACAGAG CAAGATATTT 
TTCAGAGCTG GAGTCTTGGC ACACTTAGAG GAAGAACGAG ATTTAAAAAT TACCGACATC 
ATTATCTTCT TCCAGGCTGT TTGCAGGGGT TATTTAGCCA GAAAGGCCTT CGCCAAGAAG 
CAGCAGCAGC TGAGTGCCCT GAAGGTGCTG CAGCGCAACT GTGCCGCGTA CCTGAAGCTG 
CGGCACTGGC AGTGGTGGCG CGTCTTCACC AAGGTGAAAC CACTCCTACA AGTCACTCGT 
CAAGAGGAGG AGCTCCAGGC CAAAGATGAA GAGCTGCTGA AGGTGAAAGA GAAGCAGACG 
AAGGTGGAAG GGGAGCTGGA GGAGATGGAG AGGAAGCACC AACAGCTGCT GGAGGAGAAG 
AATATCCTGG CAGAGCAGCT GCAGGCGGAG ACCGAGCTCT TTGCCGAAGC AGAGGAGATG 
AGGGCCAGAC TTGCCGCTAA GAAGCAGGAA TTAGAAGAGA TTCTCCATGA CTTGGAATCT 
AGGGTAGAAG AAGAAGAAGA AAGAAACCAA ATCCTCCAAA ATGAAAAGAA GAAAATGCAA 
GCACACATTC AGGACCTGGA GGAGCAGCTA GATGAGGAGG AGGGGGCTCG CCAGAAGCTG 
CAGCTGGAGA AGGTGACGGC GGAGGCCAAG ATCAAGAAGA TGGAAGAAGA GATTCTGCTT 
CTGGAGGACC AGAACTCCAA GTTTATCAAA GAAAAGAAAC TCATGGAAGA TCGCATTGCA 
GAATGTTCCT CTCAGCTGGC TGAAGAGGAA GAAAAGGCAA AAAACTTGGC CAAAATCAGG 
AATAAGCAAG AAGTAATGAT CTCGGATTTA GAAGAGCGCT TAAAGAAGGA GGAGAAGACG 
CGCCAGGAGC TGGAGAAGGC CAAAAGGAAA CTGGACGGGG AGACCACGGA CCTGCAAGAC 
CAGATCGCCG AGCTCCAGGC GCAGATTGAC GAGCTTAAGA TCCAGGTGGC CAAGAAAGAG 
GAGGAGCTGC AGGGCGCCCT GGCCAGGGGT GACGACGAGA CGCTACACAA GAACAACGCA 
CTAAAAGTCG TGCGAGAGCT GCAGGCCCAG ATCGCCGAGC TGCAGGAGGA CTTTGAATCT 
GAGAAGGCCT CGCGGAACAA AGCAGAGAAG CAGAAGAGGG ACTTGAGTGA GGAGCTGGAG 
GCACTCAAGA CGGAGCTGGA GGACACGCTG GACACCACGG CGGCCCAGCA GGAACTGCGT 
ACAAAACGAG AACAAGAAGT GGCGGAGCTG AAGAAGGCTC TCGAGGAGGA AACGAAGAGC 
CACGAGGCTC AGATCCAGGA CATGAGGCAG AGGCATGCGA CCGCCTTGGA GGAGCTGTCG 
GAGCAGCTGG AGCAGGCCAA GAGGTTCAAA GCAAATCTGG AAAAGAACAA GCAGGGCCTG 
GAGACAGACA ACAAGGAGCT GGCGTGCGAG GTCAAGGTGC TGCAGCAGGT CAAGGCTGAG 
TCCGAGCACA AGAGGAAGAA GCTGGACGCC CAGGTCCAGG AGCTCCACGC CAAGGTCTCA 
GAGGGAGACA GACTCAGGGT GGAGCTGGCA GAGAAAGCCA ATAAGTTACA GAATGAATTG 
GATAATGTCT CAACTCTTCT AGAAGAAGCA GAGAAGAAGG GTATTAAATT TGCTAAGGAT 
GCAGCTGGTC TGGAGTCTCA GCTCCAAGAC ACACAGGAGC TTCTTCAGGA GGAGACGCGC 
CAGAAACTGA ACCTGAGCAG TCGGATCCGG CAGCTGGAGG AGGAGAGGAG CAGCCTCCAG 
GAGCAGCAGG AGGAAGAGGA GGAGGCCAGG AGGAGCCTGG AGAAGCAGCT GCAGGCCCTG 
CAGGCCCAGT TGACGGATAC CAAGAAGAAA GTAGATGATG ACCTGGGGAC AATTGAAAAT 
TTGGAAGAAG CCAAGAAGAA GCTCCTCAAG GATGTGGAGG TGCTGAGCCA GCGCCTGGAG 
GAGAAGGCCC TGGCCTATGA CAAGCTGGAG AAGACCAAGA CCCGCCTGCA GCAGGAGCTG 
GATGACCTGC TGGTAGACCT GGACCACCAG CGCCAGATCG TCTCCAACTT GGAGAAGAAG 
CAGAAGAAGT TTGATCAGCT GTTAGCGGAA GAGAAGAACA TCTCTGCCCG CTACGCAGAA 
GAGCGCGACC GGGCAGAGGC AGAGGCCCGA GAGAAGGAGA CCAAAGCCCT GTCCCTGGCC 
CGCGCCCTGG AGGAGGCCCT GGAGGCCAGG GAGGAGGCTG AGCGACAGAA TAAGCAGCTG 
CGGGCGGACA TGGAGGACCT CATGAGCTCC AAGGACGATG TGGGCAAGAA CGTTCATGAA 
CTTGAGAAAT CCAAACGGGC CCTAGAGCAG CAGGTGGAGG AAATGAGGAC CCAACTGGAG 
GAGCTGGAAG ATGAGCTCCA GGCTACAGAA GACGCCAAGC TTCGCCTAGA GGTCAACATG 
CAAGCCATGA AAGCCCAGTT TGAGAGAGAC TTGCAGACCA GGGATGAGCA GAATGAGGAA 
AAGAAGCGGC TGCTCATTAA ACAGGTGCGG GAGCTTGAGG CAGAGCTGGA GGATGAGCGG 
AAACAGCGGG CCCTGGCTGT TGCCTCGAAG AAGAAAATGG AAATAGACCT GAAGGACCTG 
GAGGCTCAAA TCGAGGCAGC TAACAAAGCA AGGGATGAAG TGATCAAGCA GCTCCGCAAA 
CTCCAGGCTC AGATGAAGGA CTACCAGCGT GAATTAGAAG AAGCTCGTGC ATCCCGGGAT 
GAGATTTTTG CTCAATCCAA AGAGAGTGAG AAGAAACTGA AAAGCCTGGA AGCAGAAATC 
CTTCAGTTGC AGGAGGAACT TGCCTCATCC GAGCGAGCCC GCCGACATGC TGAGCAGGAG 
AGAGACGAGC TGGCGGACGA GATTGCCAAT AGCGCCTCCG GCAAGTCTGC ACTCCTGGAT 
GAGAAGCGGC GTCTGGAGGC TCGGATCGCA CAGCTAGAGG AGGAGCTGGA GGAGGAGCAG 
AGCAACATGG AGCTGCTCAA TGACCGCTTC CGCAAGACCA CACTGCAGGT GGACACGCTG 
AACACTGAGC TGGCGGCCGA GCGCAGCGCT GCCCAGAAGA GCGATAATGC GCGCCAGCAG 
CTGGAGCGGC AGAACAAGGA GTTGAAGGCC AAGCTGCAGG AGCTGGAAGG CGCTGTCAAG 
TCCAAGTTCA AGGCCACCAT CTCTGCTCTA GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG 
CTTGAGCAGG AAGCCAAGGA AAGAGCCGCT GCCAATAAAC TAGTCCGTCG TACCGAGAAG 
AAGCTGAAAG AAATCTTCAT GCAGGTTGAA GATGAACGCC GCCATGCTGA CCAGTATAAA 
GAACAGATGG AGAAGGCCAA TGCCAGGATG AAGCAGCTCA AGCGGCAGCT GGAGGAAGCA 
GAGGAAGAAG CTACACGTGC CAACGCATCT CGGCGCAAAC TCCAGCGGGA ACTGGATGAT 
GCCACTGAGG CCAACGAGGG CCTGAGCCGC GAGGTCAGCA CCCTGAAGAA CCGGCTGAGG 
CGAGGCGGCC CGATCAGCTT CTCCTCCAGC CGATCAGGCC GGCGCCAGCT GCACATTGAG 
GGCGCGTCCC TGGAGCTGTC GGACGACGAC ACAGAAAGTA AGACCAGTGA CATCAACGAG 
ACACAGCCGC CGCAGTCCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220355.1
CDS	159..6182
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVLVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPQESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDEIQNIQRASFYDSVSGLHEPPVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKEKQTKVEGELEEMERKHQQLLEEKNILAEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKIQVAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETKSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSTLLEEAEKKGIKFAKDAAGLESQLQDTQELLQEETRQKLNLSSRIRQLEEERSSLQEQQEEEEEARRSLEKQLQALQAQLTDTKKKVDDDLGTIENLEEAKKKLLKDVEVLSQRLEEKALAYDKLEKTKTRLQQELDDLLVDLDHQRQIVSNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALEAREEAERQNKQLRADMEDLMSSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLIKQVRELEAELEDERKQRALAVASKKKMEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNTELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDINETQPPQSE

Target ORF information:

RefSeq Version	XM_005220298.4
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin heavy chain 10 (MYH10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220298.4

ORF Insert Sequence:

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTCAGGAAT CACCTAAACC AGTGAAACAC 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC ATTTGGAAAC 
GCAAAGACTG TGAAAAATGA TAACTCATCT CGTTTTGGCA AGTTTATTCG GATCAACTTT 
GATGTAACTG GCTATATCGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAATT GTTATCTGGA 
GCAGGAGAAC ATCTGAAGTC TGATTTGCTC CTTGAAGGAT TTAATAACTA CAGATTTCTC 
TCCAATGGCT ATATTCCTAT TCCGGGACAA CAAGACAAAG ATAACTTCCA GGAGACAATG 
GAGGCAATGC ATATAATGGG CTTCTCCCAT GAAGAGATTC TGTCAATGCT TAAAGTGGTA 
TCTTCAGTGC TTCAGTTTGG AAATATTTCT TTCAAAAAGG AGCGAAACAC TGATCAAGCC 
TCCATGCCGG AAAATACAGT TGCACAGAAG CTCTGCCATC TTCTTGGCAT GAATGTGATG 
GAGTTCACTC GGGCCATTCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA CGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCA GTAGAAGCTT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GCTGGCTCGT TCACCGCATC AATAAAGCTC TGGACAGGAC CAAACGGCAA 
GGGGCCTCCT TCATTGGGAT CCTGGACATC GCTGGATTTG AGATTTTTGA GCTGAACTCA 
TTCGAGCAAC TGTGCATCAA CTACACCAAC GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAA CTTCATCGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATTGAC CTCATCGAGA GACCTGCTAA CCCTCCTGGC 
GTGCTGGCCC TTCTGGATGA AGAGTGCTGG TTCCCTAAAG CCACAGATAA GACCTTTGTT 
GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC TCCAAGTTTC AGAAACCACG TCAATTGAAA 
GACAAAGCTG ATTTTTGCAT TATCCATTAT GCAGGGAAGG TGGACTACAA GGCAGACGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAT GACAACGTGG CCACCCTCCT GCACCAGTCC 
TCTGACAGAT TCGTGGCAGA GCTCTGGAAG GATGAGATTC AGAATATTCA GAGAGCTTCT 
TTCTATGACA GTGTTTCTGG TCTTCATGAG CCACCAGTGG ACCGGATCGT GGGTCTGGAT 
CAGGTCACGG GGATGACGGA GACGGCATTC GGCTCCGCCT ATAAAACCAA GAAGGGCATG 
TTCCGCACTG TCGGGCAGCT CTACAAAGAA TCGCTCACCA AGCTGATGGC GACGCTCCGG 
AACACCAACC CGAACTTCGT GCGCTGCATC ATCCCAAATC ACGAGAAGCG GGCTGGGAAA 
CTGGACCCAC ACCTGGTGCT GGATCAGCTC CGCTGCAATG GCGTCCTGGA AGGGATCCGC 
ATCTGCCGCC AGGGCTTCCC CAACCGGATC GTTTTCCAGG AATTCAGACA GAGATACGAG 
ATCCTCACTC CAAATGCTAT CCCTAAGGGT TTCATGGATG GCAAACAGGC TTGTGAACGA 
ATGATCCGGG CTTTAGAATT GGACCCAAAC TTGTACAGAA TTGGACAGAG CAAGATATTT 
TTCAGAGCTG GAGTCTTGGC ACACTTAGAG GAAGAACGAG ATTTAAAAAT TACCGACATC 
ATTATCTTCT TCCAGGCTGT TTGCAGGGGT TATTTAGCCA GAAAGGCCTT CGCCAAGAAG 
CAGCAGCAGC TGAGTGCCCT GAAGGTGCTG CAGCGCAACT GTGCCGCGTA CCTGAAGCTG 
CGGCACTGGC AGTGGTGGCG CGTCTTCACC AAGGTGAAAC CACTCCTACA AGTCACTCGT 
CAAGAGGAGG AGCTCCAGGC CAAAGATGAA GAGCTGCTGA AGGTGAAAGA GAAGCAGACG 
AAGGTGGAAG GGGAGCTGGA GGAGATGGAG AGGAAGCACC AACAGCTGCT GGAGGAGAAG 
AATATCCTGG CAGAGCAGCT GCAGGCGGAG ACCGAGCTCT TTGCCGAAGC AGAGGAGATG 
AGGGCCAGAC TTGCCGCTAA GAAGCAGGAA TTAGAAGAGA TTCTCCATGA CTTGGAATCT 
AGGGTAGAAG AAGAAGAAGA AAGAAACCAA ATCCTCCAAA ATGAAAAGAA GAAAATGCAA 
GCACACATTC AGGACCTGGA GGAGCAGCTA GATGAGGAGG AGGGGGCTCG CCAGAAGCTG 
CAGCTGGAGA AGGTGACGGC GGAGGCCAAG ATCAAGAAGA TGGAAGAAGA GATTCTGCTT 
CTGGAGGACC AGAACTCCAA GTTTATCAAA GAAAAGAAAC TCATGGAAGA TCGCATTGCA 
GAATGTTCCT CTCAGCTGGC TGAAGAGGAA GAAAAGGCAA AAAACTTGGC CAAAATCAGG 
AATAAGCAAG AAGTAATGAT CTCGGATTTA GAAGAGCGCT TAAAGAAGGA GGAGAAGACG 
CGCCAGGAGC TGGAGAAGGC CAAAAGGAAA CTGGACGGGG AGACCACGGA CCTGCAAGAC 
CAGATCGCCG AGCTCCAGGC GCAGATTGAC GAGCTTAAGA TCCAGGTGGC CAAGAAAGAG 
GAGGAGCTGC AGGGCGCCCT GGCCAGGGGT GACGACGAGA CGCTACACAA GAACAACGCA 
CTAAAAGTCG TGCGAGAGCT GCAGGCCCAG ATCGCCGAGC TGCAGGAGGA CTTTGAATCT 
GAGAAGGCCT CGCGGAACAA AGCAGAGAAG CAGAAGAGGG ACTTGAGTGA GGAGCTGGAG 
GCACTCAAGA CGGAGCTGGA GGACACGCTG GACACCACGG CGGCCCAGCA GGAACTGCGT 
ACAAAACGAG AACAAGAAGT GGCGGAGCTG AAGAAGGCTC TCGAGGAGGA AACGAAGAGC 
CACGAGGCTC AGATCCAGGA CATGAGGCAG AGGCATGCGA CCGCCTTGGA GGAGCTGTCG 
GAGCAGCTGG AGCAGGCCAA GAGGTTCAAA GCAAATCTGG AAAAGAACAA GCAGGGCCTG 
GAGACAGACA ACAAGGAGCT GGCGTGCGAG GTCAAGGTGC TGCAGCAGGT CAAGGCTGAG 
TCCGAGCACA AGAGGAAGAA GCTGGACGCC CAGGTCCAGG AGCTCCACGC CAAGGTCTCA 
GAGGGAGACA GACTCAGGGT GGAGCTGGCA GAGAAAGCCA ATAAGTTACA GAATGAATTG 
GATAATGTCT CAACTCTTCT AGAAGAAGCA GAGAAGAAGG GTATTAAATT TGCTAAGGAT 
GCAGCTGGTC TGGAGTCTCA GCTCCAAGAC ACACAGGAGC TTCTTCAGGA GGAGACGCGC 
CAGAAACTGA ACCTGAGCAG TCGGATCCGG CAGCTGGAGG AGGAGAGGAG CAGCCTCCAG 
GAGCAGCAGG AGGAAGAGGA GGAGGCCAGG AGGAGCCTGG AGAAGCAGCT GCAGGCCCTG 
CAGGCCCAGT TGACGGATAC CAAGAAGAAA GTAGATGATG ACCTGGGGAC AATTGAAAAT 
TTGGAAGAAG CCAAGAAGAA GCTCCTCAAG GATGTGGAGG TGCTGAGCCA GCGCCTGGAG 
GAGAAGGCCC TGGCCTATGA CAAGCTGGAG AAGACCAAGA CCCGCCTGCA GCAGGAGCTG 
GATGACCTGC TGGTAGACCT GGACCACCAG CGCCAGATCG TCTCCAACTT GGAGAAGAAG 
CAGAAGAAGT TTGATCAGCT GTTAGCGGAA GAGAAGAACA TCTCTGCCCG CTACGCAGAA 
GAGCGCGACC GGGCAGAGGC AGAGGCCCGA GAGAAGGAGA CCAAAGCCCT GTCCCTGGCC 
CGCGCCCTGG AGGAGGCCCT GGAGGCCAGG GAGGAGGCTG AGCGACAGAA TAAGCAGCTG 
CGGGCGGACA TGGAGGACCT CATGAGCTCC AAGGACGATG TGGGCAAGAA CGTTCATGAA 
CTTGAGAAAT CCAAACGGGC CCTAGAGCAG CAGGTGGAGG AAATGAGGAC CCAACTGGAG 
GAGCTGGAAG ATGAGCTCCA GGCTACAGAA GACGCCAAGC TTCGCCTAGA GGTCAACATG 
CAAGCCATGA AAGCCCAGTT TGAGAGAGAC TTGCAGACCA GGGATGAGCA GAATGAGGAA 
AAGAAGCGGC TGCTCATTAA ACAGGTGCGG GAGCTTGAGG CAGAGCTGGA GGATGAGCGG 
AAACAGCGGG CCCTGGCTGT TGCCTCGAAG AAGAAAATGG AAATAGACCT GAAGGACCTG 
GAGGCTCAAA TCGAGGCAGC TAACAAAGCA AGGGATGAAG TGATCAAGCA GCTCCGCAAA 
CTCCAGGCTC AGATGAAGGA CTACCAGCGT GAATTAGAAG AAGCTCGTGC ATCCCGGGAT 
GAGATTTTTG CTCAATCCAA AGAGAGTGAG AAGAAACTGA AAAGCCTGGA AGCAGAAATC 
CTTCAGTTGC AGGAGGAACT TGCCTCATCC GAGCGAGCCC GCCGACATGC TGAGCAGGAG 
AGAGACGAGC TGGCGGACGA GATTGCCAAT AGCGCCTCCG GCAAGTCTGC ACTCCTGGAT 
GAGAAGCGGC GTCTGGAGGC TCGGATCGCA CAGCTAGAGG AGGAGCTGGA GGAGGAGCAG 
AGCAACATGG AGCTGCTCAA TGACCGCTTC CGCAAGACCA CACTGCAGGT GGACACGCTG 
AACACTGAGC TGGCGGCCGA GCGCAGCGCT GCCCAGAAGA GCGATAATGC GCGCCAGCAG 
CTGGAGCGGC AGAACAAGGA GTTGAAGGCC AAGCTGCAGG AGCTGGAAGG CGCTGTCAAG 
TCCAAGTTCA AGGCCACCAT CTCTGCTCTA GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG 
CTTGAGCAGG AAGCCAAGGA AAGAGCCGCT GCCAATAAAC TAGTCCGTCG TACCGAGAAG 
AAGCTGAAAG AAATCTTCAT GCAGGTTGAA GATGAACGCC GCCATGCTGA CCAGTATAAA 
GAACAGATGG AGAAGGCCAA TGCCAGGATG AAGCAGCTCA AGCGGCAGCT GGAGGAAGCA 
GAGGAAGAAG CTACACGTGC CAACGCATCT CGGCGCAAAC TCCAGCGGGA ACTGGATGAT 
GCCACTGAGG CCAACGAGGG CCTGAGCCGC GAGGTCAGCA CCCTGAAGAA CCGGCTGAGG 
CGAGGCGGCC CGATCAGCTT CTCCTCCAGC CGATCAGGCC GGCGCCAGCT GCACATTGAG 
GGCGCGTCCC TGGAGCTGTC GGACGACGAC ACAGAAAGTA AGACCAGTGA CATCAACGAG 
ACACAGCCGC CGCAGTCCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_005220297.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220354.1 Bos taurus myosin heavy chain 10 (MYH10), transcript variant X1, mRNA.

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CloneID	OBa226015
Clone ID Related Accession (Same CDS sequence)	XM_005220298.4 , XM_005220297.4
Accession Version	XM_005220297.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myosin-10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220297.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTCAGGAAT CACCTAAACC AGTGAAACAC 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC ATTTGGAAAC 
GCAAAGACTG TGAAAAATGA TAACTCATCT CGTTTTGGCA AGTTTATTCG GATCAACTTT 
GATGTAACTG GCTATATCGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAATT GTTATCTGGA 
GCAGGAGAAC ATCTGAAGTC TGATTTGCTC CTTGAAGGAT TTAATAACTA CAGATTTCTC 
TCCAATGGCT ATATTCCTAT TCCGGGACAA CAAGACAAAG ATAACTTCCA GGAGACAATG 
GAGGCAATGC ATATAATGGG CTTCTCCCAT GAAGAGATTC TGTCAATGCT TAAAGTGGTA 
TCTTCAGTGC TTCAGTTTGG AAATATTTCT TTCAAAAAGG AGCGAAACAC TGATCAAGCC 
TCCATGCCGG AAAATACAGT TGCACAGAAG CTCTGCCATC TTCTTGGCAT GAATGTGATG 
GAGTTCACTC GGGCCATTCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA CGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCA GTAGAAGCTT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GCTGGCTCGT TCACCGCATC AATAAAGCTC TGGACAGGAC CAAACGGCAA 
GGGGCCTCCT TCATTGGGAT CCTGGACATC GCTGGATTTG AGATTTTTGA GCTGAACTCA 
TTCGAGCAAC TGTGCATCAA CTACACCAAC GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAA CTTCATCGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATTGAC CTCATCGAGA GACCTGCTAA CCCTCCTGGC 
GTGCTGGCCC TTCTGGATGA AGAGTGCTGG TTCCCTAAAG CCACAGATAA GACCTTTGTT 
GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC TCCAAGTTTC AGAAACCACG TCAATTGAAA 
GACAAAGCTG ATTTTTGCAT TATCCATTAT GCAGGGAAGG TGGACTACAA GGCAGACGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAT GACAACGTGG CCACCCTCCT GCACCAGTCC 
TCTGACAGAT TCGTGGCAGA GCTCTGGAAG GATGAGATTC AGAATATTCA GAGAGCTTCT 
TTCTATGACA GTGTTTCTGG TCTTCATGAG CCACCAGTGG ACCGGATCGT GGGTCTGGAT 
CAGGTCACGG GGATGACGGA GACGGCATTC GGCTCCGCCT ATAAAACCAA GAAGGGCATG 
TTCCGCACTG TCGGGCAGCT CTACAAAGAA TCGCTCACCA AGCTGATGGC GACGCTCCGG 
AACACCAACC CGAACTTCGT GCGCTGCATC ATCCCAAATC ACGAGAAGCG GGCTGGGAAA 
CTGGACCCAC ACCTGGTGCT GGATCAGCTC CGCTGCAATG GCGTCCTGGA AGGGATCCGC 
ATCTGCCGCC AGGGCTTCCC CAACCGGATC GTTTTCCAGG AATTCAGACA GAGATACGAG 
ATCCTCACTC CAAATGCTAT CCCTAAGGGT TTCATGGATG GCAAACAGGC TTGTGAACGA 
ATGATCCGGG CTTTAGAATT GGACCCAAAC TTGTACAGAA TTGGACAGAG CAAGATATTT 
TTCAGAGCTG GAGTCTTGGC ACACTTAGAG GAAGAACGAG ATTTAAAAAT TACCGACATC 
ATTATCTTCT TCCAGGCTGT TTGCAGGGGT TATTTAGCCA GAAAGGCCTT CGCCAAGAAG 
CAGCAGCAGC TGAGTGCCCT GAAGGTGCTG CAGCGCAACT GTGCCGCGTA CCTGAAGCTG 
CGGCACTGGC AGTGGTGGCG CGTCTTCACC AAGGTGAAAC CACTCCTACA AGTCACTCGT 
CAAGAGGAGG AGCTCCAGGC CAAAGATGAA GAGCTGCTGA AGGTGAAAGA GAAGCAGACG 
AAGGTGGAAG GGGAGCTGGA GGAGATGGAG AGGAAGCACC AACAGCTGCT GGAGGAGAAG 
AATATCCTGG CAGAGCAGCT GCAGGCGGAG ACCGAGCTCT TTGCCGAAGC AGAGGAGATG 
AGGGCCAGAC TTGCCGCTAA GAAGCAGGAA TTAGAAGAGA TTCTCCATGA CTTGGAATCT 
AGGGTAGAAG AAGAAGAAGA AAGAAACCAA ATCCTCCAAA ATGAAAAGAA GAAAATGCAA 
GCACACATTC AGGACCTGGA GGAGCAGCTA GATGAGGAGG AGGGGGCTCG CCAGAAGCTG 
CAGCTGGAGA AGGTGACGGC GGAGGCCAAG ATCAAGAAGA TGGAAGAAGA GATTCTGCTT 
CTGGAGGACC AGAACTCCAA GTTTATCAAA GAAAAGAAAC TCATGGAAGA TCGCATTGCA 
GAATGTTCCT CTCAGCTGGC TGAAGAGGAA GAAAAGGCAA AAAACTTGGC CAAAATCAGG 
AATAAGCAAG AAGTAATGAT CTCGGATTTA GAAGAGCGCT TAAAGAAGGA GGAGAAGACG 
CGCCAGGAGC TGGAGAAGGC CAAAAGGAAA CTGGACGGGG AGACCACGGA CCTGCAAGAC 
CAGATCGCCG AGCTCCAGGC GCAGATTGAC GAGCTTAAGA TCCAGGTGGC CAAGAAAGAG 
GAGGAGCTGC AGGGCGCCCT GGCCAGGGGT GACGACGAGA CGCTACACAA GAACAACGCA 
CTAAAAGTCG TGCGAGAGCT GCAGGCCCAG ATCGCCGAGC TGCAGGAGGA CTTTGAATCT 
GAGAAGGCCT CGCGGAACAA AGCAGAGAAG CAGAAGAGGG ACTTGAGTGA GGAGCTGGAG 
GCACTCAAGA CGGAGCTGGA GGACACGCTG GACACCACGG CGGCCCAGCA GGAACTGCGT 
ACAAAACGAG AACAAGAAGT GGCGGAGCTG AAGAAGGCTC TCGAGGAGGA AACGAAGAGC 
CACGAGGCTC AGATCCAGGA CATGAGGCAG AGGCATGCGA CCGCCTTGGA GGAGCTGTCG 
GAGCAGCTGG AGCAGGCCAA GAGGTTCAAA GCAAATCTGG AAAAGAACAA GCAGGGCCTG 
GAGACAGACA ACAAGGAGCT GGCGTGCGAG GTCAAGGTGC TGCAGCAGGT CAAGGCTGAG 
TCCGAGCACA AGAGGAAGAA GCTGGACGCC CAGGTCCAGG AGCTCCACGC CAAGGTCTCA 
GAGGGAGACA GACTCAGGGT GGAGCTGGCA GAGAAAGCCA ATAAGTTACA GAATGAATTG 
GATAATGTCT CAACTCTTCT AGAAGAAGCA GAGAAGAAGG GTATTAAATT TGCTAAGGAT 
GCAGCTGGTC TGGAGTCTCA GCTCCAAGAC ACACAGGAGC TTCTTCAGGA GGAGACGCGC 
CAGAAACTGA ACCTGAGCAG TCGGATCCGG CAGCTGGAGG AGGAGAGGAG CAGCCTCCAG 
GAGCAGCAGG AGGAAGAGGA GGAGGCCAGG AGGAGCCTGG AGAAGCAGCT GCAGGCCCTG 
CAGGCCCAGT TGACGGATAC CAAGAAGAAA GTAGATGATG ACCTGGGGAC AATTGAAAAT 
TTGGAAGAAG CCAAGAAGAA GCTCCTCAAG GATGTGGAGG TGCTGAGCCA GCGCCTGGAG 
GAGAAGGCCC TGGCCTATGA CAAGCTGGAG AAGACCAAGA CCCGCCTGCA GCAGGAGCTG 
GATGACCTGC TGGTAGACCT GGACCACCAG CGCCAGATCG TCTCCAACTT GGAGAAGAAG 
CAGAAGAAGT TTGATCAGCT GTTAGCGGAA GAGAAGAACA TCTCTGCCCG CTACGCAGAA 
GAGCGCGACC GGGCAGAGGC AGAGGCCCGA GAGAAGGAGA CCAAAGCCCT GTCCCTGGCC 
CGCGCCCTGG AGGAGGCCCT GGAGGCCAGG GAGGAGGCTG AGCGACAGAA TAAGCAGCTG 
CGGGCGGACA TGGAGGACCT CATGAGCTCC AAGGACGATG TGGGCAAGAA CGTTCATGAA 
CTTGAGAAAT CCAAACGGGC CCTAGAGCAG CAGGTGGAGG AAATGAGGAC CCAACTGGAG 
GAGCTGGAAG ATGAGCTCCA GGCTACAGAA GACGCCAAGC TTCGCCTAGA GGTCAACATG 
CAAGCCATGA AAGCCCAGTT TGAGAGAGAC TTGCAGACCA GGGATGAGCA GAATGAGGAA 
AAGAAGCGGC TGCTCATTAA ACAGGTGCGG GAGCTTGAGG CAGAGCTGGA GGATGAGCGG 
AAACAGCGGG CCCTGGCTGT TGCCTCGAAG AAGAAAATGG AAATAGACCT GAAGGACCTG 
GAGGCTCAAA TCGAGGCAGC TAACAAAGCA AGGGATGAAG TGATCAAGCA GCTCCGCAAA 
CTCCAGGCTC AGATGAAGGA CTACCAGCGT GAATTAGAAG AAGCTCGTGC ATCCCGGGAT 
GAGATTTTTG CTCAATCCAA AGAGAGTGAG AAGAAACTGA AAAGCCTGGA AGCAGAAATC 
CTTCAGTTGC AGGAGGAACT TGCCTCATCC GAGCGAGCCC GCCGACATGC TGAGCAGGAG 
AGAGACGAGC TGGCGGACGA GATTGCCAAT AGCGCCTCCG GCAAGTCTGC ACTCCTGGAT 
GAGAAGCGGC GTCTGGAGGC TCGGATCGCA CAGCTAGAGG AGGAGCTGGA GGAGGAGCAG 
AGCAACATGG AGCTGCTCAA TGACCGCTTC CGCAAGACCA CACTGCAGGT GGACACGCTG 
AACACTGAGC TGGCGGCCGA GCGCAGCGCT GCCCAGAAGA GCGATAATGC GCGCCAGCAG 
CTGGAGCGGC AGAACAAGGA GTTGAAGGCC AAGCTGCAGG AGCTGGAAGG CGCTGTCAAG 
TCCAAGTTCA AGGCCACCAT CTCTGCTCTA GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG 
CTTGAGCAGG AAGCCAAGGA AAGAGCCGCT GCCAATAAAC TAGTCCGTCG TACCGAGAAG 
AAGCTGAAAG AAATCTTCAT GCAGGTTGAA GATGAACGCC GCCATGCTGA CCAGTATAAA 
GAACAGATGG AGAAGGCCAA TGCCAGGATG AAGCAGCTCA AGCGGCAGCT GGAGGAAGCA 
GAGGAAGAAG CTACACGTGC CAACGCATCT CGGCGCAAAC TCCAGCGGGA ACTGGATGAT 
GCCACTGAGG CCAACGAGGG CCTGAGCCGC GAGGTCAGCA CCCTGAAGAA CCGGCTGAGG 
CGAGGCGGCC CGATCAGCTT CTCCTCCAGC CGATCAGGCC GGCGCCAGCT GCACATTGAG 
GGCGCGTCCC TGGAGCTGTC GGACGACGAC ACAGAAAGTA AGACCAGTGA CATCAACGAG 
ACACAGCCGC CGCAGTCCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220354.1
CDS	309..6332
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVLVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPQESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDEIQNIQRASFYDSVSGLHEPPVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKEKQTKVEGELEEMERKHQQLLEEKNILAEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKIQVAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETKSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSTLLEEAEKKGIKFAKDAAGLESQLQDTQELLQEETRQKLNLSSRIRQLEEERSSLQEQQEEEEEARRSLEKQLQALQAQLTDTKKKVDDDLGTIENLEEAKKKLLKDVEVLSQRLEEKALAYDKLEKTKTRLQQELDDLLVDLDHQRQIVSNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALEAREEAERQNKQLRADMEDLMSSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLIKQVRELEAELEDERKQRALAVASKKKMEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNTELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDINETQPPQSE

Target ORF information:

RefSeq Version	XM_005220297.4
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin heavy chain 10 (MYH10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220297.4

ORF Insert Sequence:

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTCAGGAAT CACCTAAACC AGTGAAACAC 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC ATTTGGAAAC 
GCAAAGACTG TGAAAAATGA TAACTCATCT CGTTTTGGCA AGTTTATTCG GATCAACTTT 
GATGTAACTG GCTATATCGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAATT GTTATCTGGA 
GCAGGAGAAC ATCTGAAGTC TGATTTGCTC CTTGAAGGAT TTAATAACTA CAGATTTCTC 
TCCAATGGCT ATATTCCTAT TCCGGGACAA CAAGACAAAG ATAACTTCCA GGAGACAATG 
GAGGCAATGC ATATAATGGG CTTCTCCCAT GAAGAGATTC TGTCAATGCT TAAAGTGGTA 
TCTTCAGTGC TTCAGTTTGG AAATATTTCT TTCAAAAAGG AGCGAAACAC TGATCAAGCC 
TCCATGCCGG AAAATACAGT TGCACAGAAG CTCTGCCATC TTCTTGGCAT GAATGTGATG 
GAGTTCACTC GGGCCATTCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA CGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCA GTAGAAGCTT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GCTGGCTCGT TCACCGCATC AATAAAGCTC TGGACAGGAC CAAACGGCAA 
GGGGCCTCCT TCATTGGGAT CCTGGACATC GCTGGATTTG AGATTTTTGA GCTGAACTCA 
TTCGAGCAAC TGTGCATCAA CTACACCAAC GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAA CTTCATCGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATTGAC CTCATCGAGA GACCTGCTAA CCCTCCTGGC 
GTGCTGGCCC TTCTGGATGA AGAGTGCTGG TTCCCTAAAG CCACAGATAA GACCTTTGTT 
GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC TCCAAGTTTC AGAAACCACG TCAATTGAAA 
GACAAAGCTG ATTTTTGCAT TATCCATTAT GCAGGGAAGG TGGACTACAA GGCAGACGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAT GACAACGTGG CCACCCTCCT GCACCAGTCC 
TCTGACAGAT TCGTGGCAGA GCTCTGGAAG GATGAGATTC AGAATATTCA GAGAGCTTCT 
TTCTATGACA GTGTTTCTGG TCTTCATGAG CCACCAGTGG ACCGGATCGT GGGTCTGGAT 
CAGGTCACGG GGATGACGGA GACGGCATTC GGCTCCGCCT ATAAAACCAA GAAGGGCATG 
TTCCGCACTG TCGGGCAGCT CTACAAAGAA TCGCTCACCA AGCTGATGGC GACGCTCCGG 
AACACCAACC CGAACTTCGT GCGCTGCATC ATCCCAAATC ACGAGAAGCG GGCTGGGAAA 
CTGGACCCAC ACCTGGTGCT GGATCAGCTC CGCTGCAATG GCGTCCTGGA AGGGATCCGC 
ATCTGCCGCC AGGGCTTCCC CAACCGGATC GTTTTCCAGG AATTCAGACA GAGATACGAG 
ATCCTCACTC CAAATGCTAT CCCTAAGGGT TTCATGGATG GCAAACAGGC TTGTGAACGA 
ATGATCCGGG CTTTAGAATT GGACCCAAAC TTGTACAGAA TTGGACAGAG CAAGATATTT 
TTCAGAGCTG GAGTCTTGGC ACACTTAGAG GAAGAACGAG ATTTAAAAAT TACCGACATC 
ATTATCTTCT TCCAGGCTGT TTGCAGGGGT TATTTAGCCA GAAAGGCCTT CGCCAAGAAG 
CAGCAGCAGC TGAGTGCCCT GAAGGTGCTG CAGCGCAACT GTGCCGCGTA CCTGAAGCTG 
CGGCACTGGC AGTGGTGGCG CGTCTTCACC AAGGTGAAAC CACTCCTACA AGTCACTCGT 
CAAGAGGAGG AGCTCCAGGC CAAAGATGAA GAGCTGCTGA AGGTGAAAGA GAAGCAGACG 
AAGGTGGAAG GGGAGCTGGA GGAGATGGAG AGGAAGCACC AACAGCTGCT GGAGGAGAAG 
AATATCCTGG CAGAGCAGCT GCAGGCGGAG ACCGAGCTCT TTGCCGAAGC AGAGGAGATG 
AGGGCCAGAC TTGCCGCTAA GAAGCAGGAA TTAGAAGAGA TTCTCCATGA CTTGGAATCT 
AGGGTAGAAG AAGAAGAAGA AAGAAACCAA ATCCTCCAAA ATGAAAAGAA GAAAATGCAA 
GCACACATTC AGGACCTGGA GGAGCAGCTA GATGAGGAGG AGGGGGCTCG CCAGAAGCTG 
CAGCTGGAGA AGGTGACGGC GGAGGCCAAG ATCAAGAAGA TGGAAGAAGA GATTCTGCTT 
CTGGAGGACC AGAACTCCAA GTTTATCAAA GAAAAGAAAC TCATGGAAGA TCGCATTGCA 
GAATGTTCCT CTCAGCTGGC TGAAGAGGAA GAAAAGGCAA AAAACTTGGC CAAAATCAGG 
AATAAGCAAG AAGTAATGAT CTCGGATTTA GAAGAGCGCT TAAAGAAGGA GGAGAAGACG 
CGCCAGGAGC TGGAGAAGGC CAAAAGGAAA CTGGACGGGG AGACCACGGA CCTGCAAGAC 
CAGATCGCCG AGCTCCAGGC GCAGATTGAC GAGCTTAAGA TCCAGGTGGC CAAGAAAGAG 
GAGGAGCTGC AGGGCGCCCT GGCCAGGGGT GACGACGAGA CGCTACACAA GAACAACGCA 
CTAAAAGTCG TGCGAGAGCT GCAGGCCCAG ATCGCCGAGC TGCAGGAGGA CTTTGAATCT 
GAGAAGGCCT CGCGGAACAA AGCAGAGAAG CAGAAGAGGG ACTTGAGTGA GGAGCTGGAG 
GCACTCAAGA CGGAGCTGGA GGACACGCTG GACACCACGG CGGCCCAGCA GGAACTGCGT 
ACAAAACGAG AACAAGAAGT GGCGGAGCTG AAGAAGGCTC TCGAGGAGGA AACGAAGAGC 
CACGAGGCTC AGATCCAGGA CATGAGGCAG AGGCATGCGA CCGCCTTGGA GGAGCTGTCG 
GAGCAGCTGG AGCAGGCCAA GAGGTTCAAA GCAAATCTGG AAAAGAACAA GCAGGGCCTG 
GAGACAGACA ACAAGGAGCT GGCGTGCGAG GTCAAGGTGC TGCAGCAGGT CAAGGCTGAG 
TCCGAGCACA AGAGGAAGAA GCTGGACGCC CAGGTCCAGG AGCTCCACGC CAAGGTCTCA 
GAGGGAGACA GACTCAGGGT GGAGCTGGCA GAGAAAGCCA ATAAGTTACA GAATGAATTG 
GATAATGTCT CAACTCTTCT AGAAGAAGCA GAGAAGAAGG GTATTAAATT TGCTAAGGAT 
GCAGCTGGTC TGGAGTCTCA GCTCCAAGAC ACACAGGAGC TTCTTCAGGA GGAGACGCGC 
CAGAAACTGA ACCTGAGCAG TCGGATCCGG CAGCTGGAGG AGGAGAGGAG CAGCCTCCAG 
GAGCAGCAGG AGGAAGAGGA GGAGGCCAGG AGGAGCCTGG AGAAGCAGCT GCAGGCCCTG 
CAGGCCCAGT TGACGGATAC CAAGAAGAAA GTAGATGATG ACCTGGGGAC AATTGAAAAT 
TTGGAAGAAG CCAAGAAGAA GCTCCTCAAG GATGTGGAGG TGCTGAGCCA GCGCCTGGAG 
GAGAAGGCCC TGGCCTATGA CAAGCTGGAG AAGACCAAGA CCCGCCTGCA GCAGGAGCTG 
GATGACCTGC TGGTAGACCT GGACCACCAG CGCCAGATCG TCTCCAACTT GGAGAAGAAG 
CAGAAGAAGT TTGATCAGCT GTTAGCGGAA GAGAAGAACA TCTCTGCCCG CTACGCAGAA 
GAGCGCGACC GGGCAGAGGC AGAGGCCCGA GAGAAGGAGA CCAAAGCCCT GTCCCTGGCC 
CGCGCCCTGG AGGAGGCCCT GGAGGCCAGG GAGGAGGCTG AGCGACAGAA TAAGCAGCTG 
CGGGCGGACA TGGAGGACCT CATGAGCTCC AAGGACGATG TGGGCAAGAA CGTTCATGAA 
CTTGAGAAAT CCAAACGGGC CCTAGAGCAG CAGGTGGAGG AAATGAGGAC CCAACTGGAG 
GAGCTGGAAG ATGAGCTCCA GGCTACAGAA GACGCCAAGC TTCGCCTAGA GGTCAACATG 
CAAGCCATGA AAGCCCAGTT TGAGAGAGAC TTGCAGACCA GGGATGAGCA GAATGAGGAA 
AAGAAGCGGC TGCTCATTAA ACAGGTGCGG GAGCTTGAGG CAGAGCTGGA GGATGAGCGG 
AAACAGCGGG CCCTGGCTGT TGCCTCGAAG AAGAAAATGG AAATAGACCT GAAGGACCTG 
GAGGCTCAAA TCGAGGCAGC TAACAAAGCA AGGGATGAAG TGATCAAGCA GCTCCGCAAA 
CTCCAGGCTC AGATGAAGGA CTACCAGCGT GAATTAGAAG AAGCTCGTGC ATCCCGGGAT 
GAGATTTTTG CTCAATCCAA AGAGAGTGAG AAGAAACTGA AAAGCCTGGA AGCAGAAATC 
CTTCAGTTGC AGGAGGAACT TGCCTCATCC GAGCGAGCCC GCCGACATGC TGAGCAGGAG 
AGAGACGAGC TGGCGGACGA GATTGCCAAT AGCGCCTCCG GCAAGTCTGC ACTCCTGGAT 
GAGAAGCGGC GTCTGGAGGC TCGGATCGCA CAGCTAGAGG AGGAGCTGGA GGAGGAGCAG 
AGCAACATGG AGCTGCTCAA TGACCGCTTC CGCAAGACCA CACTGCAGGT GGACACGCTG 
AACACTGAGC TGGCGGCCGA GCGCAGCGCT GCCCAGAAGA GCGATAATGC GCGCCAGCAG 
CTGGAGCGGC AGAACAAGGA GTTGAAGGCC AAGCTGCAGG AGCTGGAAGG CGCTGTCAAG 
TCCAAGTTCA AGGCCACCAT CTCTGCTCTA GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG 
CTTGAGCAGG AAGCCAAGGA AAGAGCCGCT GCCAATAAAC TAGTCCGTCG TACCGAGAAG 
AAGCTGAAAG AAATCTTCAT GCAGGTTGAA GATGAACGCC GCCATGCTGA CCAGTATAAA 
GAACAGATGG AGAAGGCCAA TGCCAGGATG AAGCAGCTCA AGCGGCAGCT GGAGGAAGCA 
GAGGAAGAAG CTACACGTGC CAACGCATCT CGGCGCAAAC TCCAGCGGGA ACTGGATGAT 
GCCACTGAGG CCAACGAGGG CCTGAGCCGC GAGGTCAGCA CCCTGAAGAA CCGGCTGAGG 
CGAGGCGGCC CGATCAGCTT CTCCTCCAGC CGATCAGGCC GGCGCCAGCT GCACATTGAG 
GGCGCGTCCC TGGAGCTGTC GGACGACGAC ACAGAAAGTA AGACCAGTGA CATCAACGAG 
ACACAGCCGC CGCAGTCCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( NM_174834.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777259.1 Bos taurus myosin heavy chain 10 (MYH10), mRNA.

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CloneID	OBa43707
Clone ID Related Accession (Same CDS sequence)	NM_174834.1
Accession Version	NM_174834.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	myosin-10
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB022023.1. Sequence Note: This sequence has been modified as follows: removed 17 bp suspected to be vector contamination from the 3' end. ##Evidence-Data-START## Transcript exon combination :: AB022023.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTGGGGAAC TTGAACGGCA GCTTTTGCAA 
GCAAATCCAA TCCTGGAATC ATTTGGAAAC GCAAAGACTG TGAAAAATGA TAACTCATCT 
CGTTTTGGCA AGTTTATTCG GATCAACTTT GATGTAACTG GCTATATCGT TGGGGCCAAC 
ATTGAAACAT ACCTTCTGGA AAAATCTCGT GCTGTTCGTC AAGCAAAAGA TGAGCGTACT 
TTTCATATCT TTTACCAATT GTTATCTGGA GCAGGAGAAC ATCTGAAGTC TGATTTGCTC 
CTTGAAGGAT TTAATAACTA CAGATTTCTC TCCAATGGCT ATATTCCTAT TCCGGGACAA 
CAAGACAAAG ATAACTTCCA GGAGACAATG GAGGCAATGC ATATAATGGG CTTCTCCCAT 
GAAGAGATTC TGTCAATGCT TAAAGTGGTA TCTTCAGTGC TTCAGTTTGG AAATATTTCT 
TTCAAAAAGG AGCGAAACAC TGATCAAGCC TCCATGCCGG AAAATACAGT TGCACAGAAG 
CTCTGCCATC TTCTTGGCAT GAATGTGATG GAGTTCACTC GGGCCATTCT CACCCCCCGG 
ATCAAGGTCG GCCGAGACTA CGTGCAGAAA GCCCAGACCA AAGAACAAGC AGATTTTGCA 
GTAGAAGCTT TGGCAAAAGC TACCTATGAG CGGCTCTTCC GCTGGCTCGT TCACCGCATC 
AATAAAGCTC TGGACAGGAC CAAACGGCAA GGGGCCTCCT TCATTGGGAT CCTGGACATC 
GCTGGATTTG AGATTTTTGA GCTGAACTCA TTCGAGCAAC TGTGCATCAA CTACACTAAC 
GAGAAGCTGC AGCAGCTCTT CAACCACACC ATGTTCATCC TGGAGCAGGA GGAGTACCAG 
CGCGAGGGCA TCGAGTGGAA CTTCATCGAC TTTGGCCTCG ACCTGCAGCC CTGCATTGAC 
CTCATCGAGA GACCTGCTAA CCCTCCTGGC GTGCTGGCCC TTCTGGATGA AGAGTGCTGG 
TTCCCTAAAG CCACAGATAA GACCTTTGTT GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC 
TCCAAGTTTC AGAAACCACG TCAATTGAAA GACAAAGCTG ATTTTTGCAT TATCCATTAT 
GCAGGGAAGG TGGACTACAA GGCAGACGAG TGGCTGATGA AGAACATGGA CCCCCTGAAT 
GACAACGTGG CCACCCTCCT GCACCAGTCC TCTGACAGAT TCGTGGCAGA GCTCTGGAAG 
GATGTGGACC GGATCGTGGG TCTGGATCAG GTCACGGGGA TGACGGAGAC GGCATTCGGC 
TCCGCCTATA AAACCAAGAA GGGCATGTTC CGCACTGTCG GGCAGCTCTA CAAAGAATCG 
CTCACCAAGC TGATGGCGAC GCTCCGGAAC ACCAACCCGA ACTTCGTGCG CTGCATCATC 
CCGAATCACG AGAAGCGGGC TGGGAAACTG GACCCACACC TGGTGCTGGA TCAGCTCCGC 
TGCAATGGCG TCCTGGAAGG GATCCGCATC TGCCGCCAGG GCTTCCCCAA CCGGATCGTT 
TTCCAGGAAT TCAGACAGAG ATACGAGATC CTCACTCCAA ATGCTATCCC TAAGGGTTTC 
ATGGATGGCA AACAGGCTTG TGAACGAATG ATCCGGGCTT TAGAATTGGA CCCAAACTTG 
TACAGAATTG GACAGAGCAA GATATTTTTC AGAGCTGGAG TCTTGGCACA CTTAGAGGAA 
GAACGAGATT TAAAAATTAC CGACATCATT ATCTTCTTCC AGGCTGTTTG CAGGGGTTAT 
TTAGCCAGAA AGGCCTTCGC CAAGAAGCAG CAGCAGCTGA GTGCCCTGAA GGTGCTGCAG 
CGCAACTGTG CCGCGTACCT GAAGCTGCGG CACTGGCAGT GGTGGCGCGT CTTCACCAAG 
GTGAAACCAC TCCTACAAGT CACTCGTCAA GAGGAGGAGC TCCAGGCCAA AGATGAAGAG 
CTGCTGAAGG TGAAAGAGAA GCAGACGAAG GTGGAAGGGG AGCTGGAGGA GATGGAGAGG 
AAGCACCAAC AGCTGCTGGA GGAGAAGAAT ATCCTGGCAG AGCAGCTGCA GGCGGAGACC 
GAGCTCTTTG CCGAAGCAGA GGAGATGAGG GCCAGACTTG CCGCTAAGAA GCAGGAATTA 
GAAGAGATTC TCCATGACTT GGAATCTAGG GTAGAAGAAG AAGAAGAAAG AAACCAAATC 
CTCCAAAATG AAAAGAAGAA AATGCAAGCA CACATTCAGG ACCTGGAGGA GCAGCTAGAT 
GAGGAGGAGG GGGCTCGCCA GAAGCTGCAG CTGGAGAAGG TGACGGCGGA GGCCAAGATC 
AAGAAGATGG AAGAAGAGAT TCTGCTTCTG GAGGACCAGA ACTCCAAGTT TATCAAAGAA 
AAGAAACTCA TGGAAGATCG CATTGCAGAA TGTTCCTCTC AGCTGGCTGA AGAGGAAGAA 
AAGGCAAAAA ACTTGGCCAA AATCAGGAAT AAGCAAGAAG TAATGATCTC GGATTTAGAA 
GAGCGCTTAA AGAAGGAGGA GAAGACGCGC CAGGAGCTGG AGAAGGCCAA AAGGAAACTG 
GACGGGGAGA CCACGGACCT GCAAGACCAG ATCGCCGAGC TCCAGGCGCA GATTGACGAG 
CTTAAGATCC AGGTGGCCAA GAAAGAGGAG GAGCTGCAGG GCGCCCTGGC CAGGGGTGAC 
GACGAGACGC TACACAAGAA CAACGCACTA AAAGTCGTGC GAGAGCTGCA GGCCCAGATC 
GCCGAGCTGC AGGAGGACTT TGAATCTGAG AAGGCCTCGC GGAACAAAGC AGAGAAGCAG 
AAGAGGGACT TGAGTGAGGA GCTGGAGGCA CTCAAGACGG AGCTGGAGGA CACGCTGGAC 
ACCACGGCGG CCCAGCAGGA ACTGCGTACA AAACGAGAAC AAGAAGTGGC GGAGCTGAAG 
AAGGCTCTCG AGGAGGAAAC GAAGAGCCAC GAGGCTCAGA TCCAGGACAT GAGGCAGAGG 
CATGCGACCG CCTTGGAGGA GCTGTCGGAG CAGCTGGAGC AGGCCAAGAG GTTCAAAGCA 
AATCTGGAAA AGAACAAGCA GGGCCTGGAG ACAGACAACA AGGAGCTGGC GTGCGAGGTC 
AAGGTGCTGC AGCAGGTCAA GGCTGAGTCC GAGCACAAAA GGAAGAAGCT GGACGCCCAG 
GTCCAGGAGC TCCACGCCAA GGTCTCAGAG GGAGACAGAC TCAGGGTGGA GCTGGCAGAG 
AAAGCCAATA AGTTACAGAA TGAATTGGAT AATGTCTCAA CTCTTCTAGA AGAAGCAGAG 
AAGAAGGGTA TTAAATTTGC TAAGGATGCA GCTGGTCTGG AGTCTCAGCT CCAAGACACA 
CAGGAGCTTC TTCAGGAGGA GACGCGCCAG AAACTGAACC TGAGCAGTCG GATCCGGCAG 
CTGGAGGAGG AGAGGAGCAG CCTCCAGGAG CAGCAGGAGG AAGAGGAGGA GGCCAGGAGG 
AGCCTGGAGA AGCAGCTGCA GGCCCTGCAG GCCCAGTTGA CGGATACCAA GAAGAAAGTA 
GATGATGACC TGGGGACAAT TGAAAATTTG GAAGAAGCCA AGAAGAAGCT CCTCAAGGAT 
GTGGAGGTGC TGAGCCAGCG CCTGGAGGAG AAGGCCCTGG CCTATGACAA GCTGGAGAAG 
ACCAAGACCC GCCTGCAGCA GGAGCTGGAT GACCTGCTGG TAGACCTGGA CCACCAGCGC 
CAGATCGTCT CCAACTTGGA GAAGAAGCAG AAGAAGTTTG ATCAGCTGTT AGCGGAAGAG 
AAGAACATCT CTGCCCGCTA CGCAGAAGAG CGCGACCGGG CAGAGGCAGA GGCCCGAGAG 
AAGGAGACCA AAGCCCTGTC CCTGGCCCGC GCCCTGGAGG AGGCCCTGGA GGCCAGGGAG 
GAGGCTGAGC GACAGAATAA GCAGCTGCGG GCGGACATGG AGGACCTCAT GAGCTCCAAG 
GACGATGTGG GCAAGAACGT TCATGAACTT GAGAAATCCA AACGGGCCCT AGAGCAGCAG 
GTGGAGGAAA TGAGGACCCA ACTGGAGGAG CTGGAAGATG AGCTCCAGGC TACAGAAGAC 
GCCAAGCTTC GCCTAGAGGT CAACATGCAA GCCATGAAAG CCCAGTTTGA GAGAGACTTG 
CAGACCAGGG ATGAGCAGAA TGAGGAAAAG AAGCGGCTGC TCATTAAACA GGTGCGGGAG 
CTTGAGGCAG AGCTGGAGGA TGAGCGGAAA CAGCGGGCCC TGGCTGTTGC CTCGAAGAAG 
AAAATGGAAA TAGACCTGAA GGACCTGGAG GCTCAAATCG AGGCAGCTAA CAAAGCAAGG 
GATGAAGTGA TCAAGCAGCT CCGCAAACTC CAGGCTCAGA TGAAGGACTA CCAGCGTGAA 
TTAGAAGAAG CTCGTGCATC CCGGGATGAG ATTTTTGCTC AATCCAAAGA GAGTGAGAAG 
AAACTGAAAA GCCTGGAAGC AGAAATCCTT CAGTTGCAGG AGGAACTTGC CTCATCCGAG 
CGAGCCCGCC GACATGCTGA GCAGGAGAGA GACGAGCTGG CGGACGAGAT TGCCAATAGC 
GCCTCCGGCA AGTCTGCACT CCTGGATGAG AAGCGGCGTC TGGAGGCTCG GATCGCACAG 
CTAGAGGAGG AGCTGGAGGA GGAGCAGAGC AACATGGAGC TGCTCAATGA CCGCTTCCGC 
AAGACCACAC TGCAGGTGGA CACGCTGAAC ACTGAGCTGG CGGCCGAGCG CAGCGCTGCC 
CAGAAGAGCG ATAATGCGCG CCAGCAGCTG GAGCGGCAGA ACAAGGAGTT GAAGGCCAAG 
CTGCAGGAGC TGGAAGGCGC TGTCAAGTCC AAGTTCAAGG CCACCATCTC TGCTCTAGAA 
GCCAAGATTG GGCAGCTGGA GGAGCAGCTT GAGCAGGAAG CCAAGGAAAG AGCCGCTGCC 
AATAAACTAG TCCGTCGTAC CGAGAAGAAG CTGAAAGAAA TCTTCATGCA GGTTGAAGAT 
GAACGCCGCC ATGCTGACCA GTATAAAGAA CAGATGGAGA AGGCCAATGC CAGGATGAAG 
CAGCTCAAGC GGCAGCTGGA GGAAGCAGAG GAAGAAGCTA CACGTGCCAA CGCATCTCGG 
CGCAAACTCC AGCGGGAACT GGATGATGCC ACTGAGGCCA ACGAGGGCCT GAGCCGCGAG 
GTCAGCACCC TGAAGAACCG GCTGAGGCGA GGCGGCCCGA TCAGCTTCTC CTCCAGCCGA 
TCAGGCCGGC GCCAGCTGCA CATTGAGGGC GCGTCCCTGG AGCTGTCGGA CGACGACACA 
GAAAGTAAGA CCAGTGACAT CAACGAGACA CAGCCGCCGC AGTCCGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777259.1
CDS	67..5997
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVLVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKEKQTKVEGELEEMERKHQQLLEEKNILAEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKIQVAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETKSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSTLLEEAEKKGIKFAKDAAGLESQLQDTQELLQEETRQKLNLSSRIRQLEEERSSLQEQQEEEEEARRSLEKQLQALQAQLTDTKKKVDDDLGTIENLEEAKKKLLKDVEVLSQRLEEKALAYDKLEKTKTRLQQELDDLLVDLDHQRQIVSNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALEAREEAERQNKQLRADMEDLMSSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLIKQVRELEAELEDERKQRALAVASKKKMEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNTELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDINETQPPQSE

Target ORF information:

RefSeq Version	NM_174834.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin heavy chain 10 (MYH10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174834.1

ORF Insert Sequence:

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTGGGGAAC TTGAACGGCA GCTTTTGCAA 
GCAAATCCAA TCCTGGAATC ATTTGGAAAC GCAAAGACTG TGAAAAATGA TAACTCATCT 
CGTTTTGGCA AGTTTATTCG GATCAACTTT GATGTAACTG GCTATATCGT TGGGGCCAAC 
ATTGAAACAT ACCTTCTGGA AAAATCTCGT GCTGTTCGTC AAGCAAAAGA TGAGCGTACT 
TTTCATATCT TTTACCAATT GTTATCTGGA GCAGGAGAAC ATCTGAAGTC TGATTTGCTC 
CTTGAAGGAT TTAATAACTA CAGATTTCTC TCCAATGGCT ATATTCCTAT TCCGGGACAA 
CAAGACAAAG ATAACTTCCA GGAGACAATG GAGGCAATGC ATATAATGGG CTTCTCCCAT 
GAAGAGATTC TGTCAATGCT TAAAGTGGTA TCTTCAGTGC TTCAGTTTGG AAATATTTCT 
TTCAAAAAGG AGCGAAACAC TGATCAAGCC TCCATGCCGG AAAATACAGT TGCACAGAAG 
CTCTGCCATC TTCTTGGCAT GAATGTGATG GAGTTCACTC GGGCCATTCT CACCCCCCGG 
ATCAAGGTCG GCCGAGACTA CGTGCAGAAA GCCCAGACCA AAGAACAAGC AGATTTTGCA 
GTAGAAGCTT TGGCAAAAGC TACCTATGAG CGGCTCTTCC GCTGGCTCGT TCACCGCATC 
AATAAAGCTC TGGACAGGAC CAAACGGCAA GGGGCCTCCT TCATTGGGAT CCTGGACATC 
GCTGGATTTG AGATTTTTGA GCTGAACTCA TTCGAGCAAC TGTGCATCAA CTACACTAAC 
GAGAAGCTGC AGCAGCTCTT CAACCACACC ATGTTCATCC TGGAGCAGGA GGAGTACCAG 
CGCGAGGGCA TCGAGTGGAA CTTCATCGAC TTTGGCCTCG ACCTGCAGCC CTGCATTGAC 
CTCATCGAGA GACCTGCTAA CCCTCCTGGC GTGCTGGCCC TTCTGGATGA AGAGTGCTGG 
TTCCCTAAAG CCACAGATAA GACCTTTGTT GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC 
TCCAAGTTTC AGAAACCACG TCAATTGAAA GACAAAGCTG ATTTTTGCAT TATCCATTAT 
GCAGGGAAGG TGGACTACAA GGCAGACGAG TGGCTGATGA AGAACATGGA CCCCCTGAAT 
GACAACGTGG CCACCCTCCT GCACCAGTCC TCTGACAGAT TCGTGGCAGA GCTCTGGAAG 
GATGTGGACC GGATCGTGGG TCTGGATCAG GTCACGGGGA TGACGGAGAC GGCATTCGGC 
TCCGCCTATA AAACCAAGAA GGGCATGTTC CGCACTGTCG GGCAGCTCTA CAAAGAATCG 
CTCACCAAGC TGATGGCGAC GCTCCGGAAC ACCAACCCGA ACTTCGTGCG CTGCATCATC 
CCGAATCACG AGAAGCGGGC TGGGAAACTG GACCCACACC TGGTGCTGGA TCAGCTCCGC 
TGCAATGGCG TCCTGGAAGG GATCCGCATC TGCCGCCAGG GCTTCCCCAA CCGGATCGTT 
TTCCAGGAAT TCAGACAGAG ATACGAGATC CTCACTCCAA ATGCTATCCC TAAGGGTTTC 
ATGGATGGCA AACAGGCTTG TGAACGAATG ATCCGGGCTT TAGAATTGGA CCCAAACTTG 
TACAGAATTG GACAGAGCAA GATATTTTTC AGAGCTGGAG TCTTGGCACA CTTAGAGGAA 
GAACGAGATT TAAAAATTAC CGACATCATT ATCTTCTTCC AGGCTGTTTG CAGGGGTTAT 
TTAGCCAGAA AGGCCTTCGC CAAGAAGCAG CAGCAGCTGA GTGCCCTGAA GGTGCTGCAG 
CGCAACTGTG CCGCGTACCT GAAGCTGCGG CACTGGCAGT GGTGGCGCGT CTTCACCAAG 
GTGAAACCAC TCCTACAAGT CACTCGTCAA GAGGAGGAGC TCCAGGCCAA AGATGAAGAG 
CTGCTGAAGG TGAAAGAGAA GCAGACGAAG GTGGAAGGGG AGCTGGAGGA GATGGAGAGG 
AAGCACCAAC AGCTGCTGGA GGAGAAGAAT ATCCTGGCAG AGCAGCTGCA GGCGGAGACC 
GAGCTCTTTG CCGAAGCAGA GGAGATGAGG GCCAGACTTG CCGCTAAGAA GCAGGAATTA 
GAAGAGATTC TCCATGACTT GGAATCTAGG GTAGAAGAAG AAGAAGAAAG AAACCAAATC 
CTCCAAAATG AAAAGAAGAA AATGCAAGCA CACATTCAGG ACCTGGAGGA GCAGCTAGAT 
GAGGAGGAGG GGGCTCGCCA GAAGCTGCAG CTGGAGAAGG TGACGGCGGA GGCCAAGATC 
AAGAAGATGG AAGAAGAGAT TCTGCTTCTG GAGGACCAGA ACTCCAAGTT TATCAAAGAA 
AAGAAACTCA TGGAAGATCG CATTGCAGAA TGTTCCTCTC AGCTGGCTGA AGAGGAAGAA 
AAGGCAAAAA ACTTGGCCAA AATCAGGAAT AAGCAAGAAG TAATGATCTC GGATTTAGAA 
GAGCGCTTAA AGAAGGAGGA GAAGACGCGC CAGGAGCTGG AGAAGGCCAA AAGGAAACTG 
GACGGGGAGA CCACGGACCT GCAAGACCAG ATCGCCGAGC TCCAGGCGCA GATTGACGAG 
CTTAAGATCC AGGTGGCCAA GAAAGAGGAG GAGCTGCAGG GCGCCCTGGC CAGGGGTGAC 
GACGAGACGC TACACAAGAA CAACGCACTA AAAGTCGTGC GAGAGCTGCA GGCCCAGATC 
GCCGAGCTGC AGGAGGACTT TGAATCTGAG AAGGCCTCGC GGAACAAAGC AGAGAAGCAG 
AAGAGGGACT TGAGTGAGGA GCTGGAGGCA CTCAAGACGG AGCTGGAGGA CACGCTGGAC 
ACCACGGCGG CCCAGCAGGA ACTGCGTACA AAACGAGAAC AAGAAGTGGC GGAGCTGAAG 
AAGGCTCTCG AGGAGGAAAC GAAGAGCCAC GAGGCTCAGA TCCAGGACAT GAGGCAGAGG 
CATGCGACCG CCTTGGAGGA GCTGTCGGAG CAGCTGGAGC AGGCCAAGAG GTTCAAAGCA 
AATCTGGAAA AGAACAAGCA GGGCCTGGAG ACAGACAACA AGGAGCTGGC GTGCGAGGTC 
AAGGTGCTGC AGCAGGTCAA GGCTGAGTCC GAGCACAAAA GGAAGAAGCT GGACGCCCAG 
GTCCAGGAGC TCCACGCCAA GGTCTCAGAG GGAGACAGAC TCAGGGTGGA GCTGGCAGAG 
AAAGCCAATA AGTTACAGAA TGAATTGGAT AATGTCTCAA CTCTTCTAGA AGAAGCAGAG 
AAGAAGGGTA TTAAATTTGC TAAGGATGCA GCTGGTCTGG AGTCTCAGCT CCAAGACACA 
CAGGAGCTTC TTCAGGAGGA GACGCGCCAG AAACTGAACC TGAGCAGTCG GATCCGGCAG 
CTGGAGGAGG AGAGGAGCAG CCTCCAGGAG CAGCAGGAGG AAGAGGAGGA GGCCAGGAGG 
AGCCTGGAGA AGCAGCTGCA GGCCCTGCAG GCCCAGTTGA CGGATACCAA GAAGAAAGTA 
GATGATGACC TGGGGACAAT TGAAAATTTG GAAGAAGCCA AGAAGAAGCT CCTCAAGGAT 
GTGGAGGTGC TGAGCCAGCG CCTGGAGGAG AAGGCCCTGG CCTATGACAA GCTGGAGAAG 
ACCAAGACCC GCCTGCAGCA GGAGCTGGAT GACCTGCTGG TAGACCTGGA CCACCAGCGC 
CAGATCGTCT CCAACTTGGA GAAGAAGCAG AAGAAGTTTG ATCAGCTGTT AGCGGAAGAG 
AAGAACATCT CTGCCCGCTA CGCAGAAGAG CGCGACCGGG CAGAGGCAGA GGCCCGAGAG 
AAGGAGACCA AAGCCCTGTC CCTGGCCCGC GCCCTGGAGG AGGCCCTGGA GGCCAGGGAG 
GAGGCTGAGC GACAGAATAA GCAGCTGCGG GCGGACATGG AGGACCTCAT GAGCTCCAAG 
GACGATGTGG GCAAGAACGT TCATGAACTT GAGAAATCCA AACGGGCCCT AGAGCAGCAG 
GTGGAGGAAA TGAGGACCCA ACTGGAGGAG CTGGAAGATG AGCTCCAGGC TACAGAAGAC 
GCCAAGCTTC GCCTAGAGGT CAACATGCAA GCCATGAAAG CCCAGTTTGA GAGAGACTTG 
CAGACCAGGG ATGAGCAGAA TGAGGAAAAG AAGCGGCTGC TCATTAAACA GGTGCGGGAG 
CTTGAGGCAG AGCTGGAGGA TGAGCGGAAA CAGCGGGCCC TGGCTGTTGC CTCGAAGAAG 
AAAATGGAAA TAGACCTGAA GGACCTGGAG GCTCAAATCG AGGCAGCTAA CAAAGCAAGG 
GATGAAGTGA TCAAGCAGCT CCGCAAACTC CAGGCTCAGA TGAAGGACTA CCAGCGTGAA 
TTAGAAGAAG CTCGTGCATC CCGGGATGAG ATTTTTGCTC AATCCAAAGA GAGTGAGAAG 
AAACTGAAAA GCCTGGAAGC AGAAATCCTT CAGTTGCAGG AGGAACTTGC CTCATCCGAG 
CGAGCCCGCC GACATGCTGA GCAGGAGAGA GACGAGCTGG CGGACGAGAT TGCCAATAGC 
GCCTCCGGCA AGTCTGCACT CCTGGATGAG AAGCGGCGTC TGGAGGCTCG GATCGCACAG 
CTAGAGGAGG AGCTGGAGGA GGAGCAGAGC AACATGGAGC TGCTCAATGA CCGCTTCCGC 
AAGACCACAC TGCAGGTGGA CACGCTGAAC ACTGAGCTGG CGGCCGAGCG CAGCGCTGCC 
CAGAAGAGCG ATAATGCGCG CCAGCAGCTG GAGCGGCAGA ACAAGGAGTT GAAGGCCAAG 
CTGCAGGAGC TGGAAGGCGC TGTCAAGTCC AAGTTCAAGG CCACCATCTC TGCTCTAGAA 
GCCAAGATTG GGCAGCTGGA GGAGCAGCTT GAGCAGGAAG CCAAGGAAAG AGCCGCTGCC 
AATAAACTAG TCCGTCGTAC CGAGAAGAAG CTGAAAGAAA TCTTCATGCA GGTTGAAGAT 
GAACGCCGCC ATGCTGACCA GTATAAAGAA CAGATGGAGA AGGCCAATGC CAGGATGAAG 
CAGCTCAAGC GGCAGCTGGA GGAAGCAGAG GAAGAAGCTA CACGTGCCAA CGCATCTCGG 
CGCAAACTCC AGCGGGAACT GGATGATGCC ACTGAGGCCA ACGAGGGCCT GAGCCGCGAG 
GTCAGCACCC TGAAGAACCG GCTGAGGCGA GGCGGCCCGA TCAGCTTCTC CTCCAGCCGA 
TCAGGCCGGC GCCAGCTGCA CATTGAGGGC GCGTCCCTGG AGCTGTCGGA CGACGACACA 
GAAAGTAAGA CCAGTGACAT CAACGAGACA CAGCCGCCGC AGTCCGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_005220297.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220354.1 Bos taurus myosin, heavy chain 10, non-muscle (MYH10), transcript variant X1, mRNA.

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CloneID	OBa29701
Clone ID Related Accession (Same CDS sequence)	XM_005220297.3 , XM_015458670.1 , XM_005220298.3
Accession Version	XM_005220297.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myosin-10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220297.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTCAGGAAT CACCTAAACC AGTGAAACAC 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC ATTTGGAAAC 
GCAAAGACTG TGAAAAATGA TAACTCATCT CGTTTTGGCA AGTTTATTCG GATCAACTTT 
GATGTAACTG GCTATATCGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAATT GTTATCTGGA 
GCAGGAGAAC ATCTGAAGTC TGATTTGCTC CTTGAAGGAT TTAATAACTA CAGATTTCTC 
TCCAATGGCT ATATTCCTAT TCCGGGACAA CAAGACAAAG ATAACTTCCA GGAGACAATG 
GAGGCAATGC ATATAATGGG CTTCTCCCAT GAAGAGATTC TGTCAATGCT TAAAGTGGTA 
TCTTCAGTGC TTCAGTTTGG AAATATTTCT TTCAAAAAGG AGCGAAACAC TGATCAAGCC 
TCCATGCCGG AAAATACAGT TGCACAGAAG CTCTGCCATC TTCTTGGCAT GAATGTGATG 
GAGTTCACTC GGGCCATTCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA CGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCA GTAGAAGCTT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GCTGGCTCGT TCACCGCATC AATAAAGCTC TGGACAGGAC CAAACGGCAA 
GGGGCCTCCT TCATTGGGAT CCTGGACATC GCTGGATTTG AGATTTTTGA GCTGAACTCA 
TTCGAGCAAC TGTGCATCAA CTACACCAAC GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAA CTTCATCGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATTGAC CTCATCGAGA GACCTGCTAA CCCTCCTGGC 
GTGCTGGCCC TTCTGGATGA AGAGTGCTGG TTCCCTAAAG CCACAGATAA GACCTTTGTT 
GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC TCCAAGTTTC AGAAACCACG TCAATTGAAA 
GACAAAGCTG ATTTTTGCAT TATCCATTAT GCAGGGAAGG TGGACTACAA GGCAGACGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAT GACAACGTGG CCACCCTCCT GCACCAGTCC 
TCTGACAGAT TCGTGGCAGA GCTCTGGAAG GATGAGATTC AGAATATTCA GAGAGCTTCT 
TTCTATGACA GTGTTTCTGG TCTTCATGAG CCACCAGTGG ACCGGATCGT GGGTCTGGAT 
CAGGTCACGG GGATGACGGA GACGGCATTC GGCTCCGCCT ATAAAACCAA GAAGGGCATG 
TTCCGCACTG TCGGGCAGCT CTACAAAGAA TCGCTCACCA AGCTGATGGC GACGCTCCGG 
AACACCAACC CGAACTTCGT GCGCTGCATC ATCCCAAATC ACGAGAAGCG GGCTGGGAAA 
CTGGACCCAC ACCTGGTGCT GGATCAGCTC CGCTGCAATG GCGTCCTGGA AGGGATCCGC 
ATCTGCCGCC AGGGCTTCCC CAACCGGATC GTTTTCCAGG AATTCAGACA GAGATACGAG 
ATCCTCACTC CAAATGCTAT CCCTAAGGGT TTCATGGATG GCAAACAGGC TTGTGAACGA 
ATGATCCGGG CTTTAGAATT GGACCCAAAC TTGTACAGAA TTGGACAGAG CAAGATATTT 
TTCAGAGCTG GAGTCTTGGC ACACTTAGAG GAAGAACGAG ATTTAAAAAT TACCGACATC 
ATTATCTTCT TCCAGGCTGT TTGCAGGGGT TATTTAGCCA GAAAGGCCTT CGCCAAGAAG 
CAGCAGCAGC TGAGTGCCCT GAAGGTGCTG CAGCGCAACT GTGCCGCGTA CCTGAAGCTG 
CGGCACTGGC AGTGGTGGCG CGTCTTCACC AAGGTGAAAC CACTCCTACA AGTCACTCGT 
CAAGAGGAGG AGCTCCAGGC CAAAGATGAA GAGCTGCTGA AGGTGAAAGA GAAGCAGACG 
AAGGTGGAAG GGGAGCTGGA GGAGATGGAG AGGAAGCACC AACAGCTGCT GGAGGAGAAG 
AATATCCTGG CAGAGCAGCT GCAGGCGGAG ACCGAGCTCT TTGCCGAAGC AGAGGAGATG 
AGGGCCAGAC TTGCCGCTAA GAAGCAGGAA TTAGAAGAGA TTCTCCATGA CTTGGAATCT 
AGGGTAGAAG AAGAAGAAGA AAGAAACCAA ATCCTCCAAA ATGAAAAGAA GAAAATGCAA 
GCACACATTC AGGACCTGGA GGAGCAGCTA GATGAGGAGG AGGGGGCTCG CCAGAAGCTG 
CAGCTGGAGA AGGTGACGGC GGAGGCCAAG ATCAAGAAGA TGGAAGAAGA GATTCTGCTT 
CTGGAGGACC AGAACTCCAA GTTTATCAAA GAAAAGAAAC TCATGGAAGA TCGCATTGCA 
GAATGTTCCT CTCAGCTGGC TGAAGAGGAA GAAAAGGCAA AAAACTTGGC CAAAATCAGG 
AATAAGCAAG AAGTAATGAT CTCGGATTTA GAAGAGCGCT TAAAGAAGGA GGAGAAGACG 
CGCCAGGAGC TGGAGAAGGC CAAAAGGAAA CTGGACGGGG AGACCACGGA CCTGCAAGAC 
CAGATCGCCG AGCTCCAGGC GCAGATTGAC GAGCTTAAGA TCCAGGTGGC CAAGAAAGAG 
GAGGAGCTGC AGGGTGCCCT GGCCAGGGGT GACGACGAGA CGCTACACAA GAACAACGCA 
CTAAAAGTCG TGCGAGAGCT GCAGGCCCAG ATCGCCGAGC TGCAGGAGGA CTTTGAATCT 
GAGAAGGCCT CGCGGAACAA AGCAGAGAAG CAGAAGAGGG ACTTGAGTGA GGAGCTGGAG 
GCACTCAAGA CGGAGCTGGA GGACACGCTG GACACCACGG CGGCCCAGCA GGAACTGCGT 
ACAAAACGAG AACAAGAAGT GGCGGAGCTG AAGAAGGCTC TCGAGGAGGA AACGAAGAGC 
CACGAGGCTC AGATCCAGGA CATGAGGCAG AGGCATGCGA CCGCCTTGGA GGAGCTGTCG 
GAGCAGCTGG AGCAGGCCAA GAGGTTCAAA GCAAATCTGG AAAAGAACAA GCAGGGCCTG 
GAGACAGACA ACAAGGAGCT GGCGTGCGAG GTCAAGGTGC TGCAGCAGGT CAAGGCTGAG 
TCCGAGCACA AGAGGAAGAA GCTGGACGCC CAGGTCCAGG AGCTCCACGC CAAGGTCTCA 
GAGGGAGACA GACTCAGGGT GGAGCTGGCA GAGAAAGCCA ATAAGTTACA GAATGAATTG 
GATAATGTCT CAACTCTTCT AGAAGAAGCA GAGAAGAAGG GTATTAAATT TGCTAAGGAT 
GCAGCTGGTC TGGAGTCTCA GCTCCAAGAC ACACAGGAGC TTCTTCAGGA GGAGACGCGC 
CAGAAACTGA ACCTGAGCAG TCGGATCCGG CAGCTGGAGG AGGAGAGGAG CAGCCTCCAG 
GAGCAGCAGG AGGAAGAGGA GGAGGCCAGG AGGAGCCTGG AGAAGCAGCT GCAGGCCCTG 
CAGGCCCAGT TGACGGATAC CAAGAAGAAA GTAGATGATG ACCTGGGGAC AATTGAAAAT 
TTGGAAGAAG CCAAGAAGAA GCTCCTCAAG GATGTGGAGG TGCTGAGCCA GCGCCTGGAG 
GAGAAGGCCC TGGCCTATGA CAAGCTGGAG AAGACCAAGA CCCGCCTGCA GCAGGAGCTG 
GATGACCTGC TGGTAGACCT GGACCACCAG CGCCAGATCG TCTCCAACTT GGAGAAGAAG 
CAGAAGAAGT TTGATCAGCT GTTAGCGGAA GAGAAGAACA TCTCTGCCCG CTACGCAGAA 
GAGCGCGACC GGGCAGAGGC AGAGGCCCGA GAGAAGGAGA CCAAAGCCCT GTCCCTGGCC 
CGCGCCCTGG AGGAGGCCCT GGAGGCCAGG GAGGAGGCTG AGCGACAGAA TAAGCAGCTG 
CGGGCGGACA TGGAGGACCT CATGAGCTCC AAGGACGATG TGGGCAAGAA CGTTCATGAA 
CTTGAGAAAT CCAAACGGGC CCTAGAGCAG CAGGTGGAGG AAATGAGGAC CCAACTGGAG 
GAGCTGGAAG ATGAGCTCCA GGCTACAGAA GACGCCAAGC TTCGCCTAGA GGTCAACATG 
CAAGCCATGA AAGCCCAGTT TGAGAGAGAC TTGCAGACCA GGGATGAGCA GAATGAGGAA 
AAGAAGCGGC TGCTCATTAA ACAGGTGCGG GAGCTTGAGG CAGAGCTGGA GGATGAGCGG 
AAACAGCGGG CCCTGGCTGT TGCCTCGAAG AAGAAAATGG AAATAGACCT GAAGGACCTG 
GAGGCTCAAA TCGAGGCAGC TAACAAAGCA AGGGATGAAG TGATCAAGCA GCTCCGCAAA 
CTCCAGGCTC AGATGAAGGA CTACCAGCGT GAATTAGAAG AAGCTCGTGC ATCCCGGGAT 
GAGATTTTTG CTCAATCCAA AGAGAGTGAG AAGAAACTGA AAAGCCTGGA AGCAGAAATC 
CTTCAGTTGC AGGAGGAACT TGCCTCATCC GAGCGAGCCC GCCGACATGC TGAGCAGGAG 
AGAGACGAGC TGGCGGACGA GATTGCCAAT AGCGCCTCCG GCAAGTCTGC ACTCCTGGAT 
GAGAAGCGGC GTCTGGAGGC TCGGATCGCA CAGCTAGAGG AGGAGCTGGA GGAGGAGCAG 
AGCAACATGG AGCTGCTCAA TGACCGCTTC CGCAAGACCA CACTGCAGGT GGACACGCTG 
AACACTGAGC TGGCGGCCGA GCGCAGCGCT GCCCAGAAGA GCGATAATGC GCGCCAGCAG 
CTGGAGCGGC AGAACAAGGA GTTGAAGGCC AAGCTGCAGG AGCTGGAAGG CGCTGTCAAG 
TCCAAGTTCA AGGCCACCAT CTCTGCTCTA GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG 
CTTGAGCAGG AAGCCAAGGA AAGAGCCGCT GCCAATAAAC TAGTCCGTCG TACCGAGAAG 
AAGCTGAAAG AAATCTTCAT GCAGGTTGAA GATGAACGCC GCCATGCTGA CCAGTATAAA 
GAACAGATGG AGAAGGCCAA TGCCAGGATG AAGCAGCTCA AGCGGCAGCT GGAGGAAGCA 
GAGGAAGAAG CTACACGTGC CAACGCATCT CGGCGCAAAC TCCAGCGGGA ACTGGATGAT 
GCCACTGAGG CCAACGAGGG CCTGAGCCGC GAGGTCAGCA CCCTGAAGAA CCGGCTGAGG 
CGAGGCGGCC CGATCAGCTT CTCCTCCAGC CGATCAGGCC GGCGCCAGCT GCACATTGAG 
GGCGCGTCCC TGGAGCTGTC GGACGACGAC ACAGAAAGTA AGACCAGTGA CATCAACGAG 
ACACAGCCGC CGCAGTCCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220354.1
CDS	247..6270
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVLVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPQESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDEIQNIQRASFYDSVSGLHEPPVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKEKQTKVEGELEEMERKHQQLLEEKNILAEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKIQVAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETKSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSTLLEEAEKKGIKFAKDAAGLESQLQDTQELLQEETRQKLNLSSRIRQLEEERSSLQEQQEEEEEARRSLEKQLQALQAQLTDTKKKVDDDLGTIENLEEAKKKLLKDVEVLSQRLEEKALAYDKLEKTKTRLQQELDDLLVDLDHQRQIVSNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALEAREEAERQNKQLRADMEDLMSSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLIKQVRELEAELEDERKQRALAVASKKKMEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNTELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDINETQPPQSE

Target ORF information:

RefSeq Version	XM_005220297.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin, heavy chain 10, non-muscle (MYH10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220297.3

ORF Insert Sequence:

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTCAGGAAT CACCTAAACC AGTGAAACAC 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC ATTTGGAAAC 
GCAAAGACTG TGAAAAATGA TAACTCATCT CGTTTTGGCA AGTTTATTCG GATCAACTTT 
GATGTAACTG GCTATATCGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAATT GTTATCTGGA 
GCAGGAGAAC ATCTGAAGTC TGATTTGCTC CTTGAAGGAT TTAATAACTA CAGATTTCTC 
TCCAATGGCT ATATTCCTAT TCCGGGACAA CAAGACAAAG ATAACTTCCA GGAGACAATG 
GAGGCAATGC ATATAATGGG CTTCTCCCAT GAAGAGATTC TGTCAATGCT TAAAGTGGTA 
TCTTCAGTGC TTCAGTTTGG AAATATTTCT TTCAAAAAGG AGCGAAACAC TGATCAAGCC 
TCCATGCCGG AAAATACAGT TGCACAGAAG CTCTGCCATC TTCTTGGCAT GAATGTGATG 
GAGTTCACTC GGGCCATTCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA CGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCA GTAGAAGCTT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GCTGGCTCGT TCACCGCATC AATAAAGCTC TGGACAGGAC CAAACGGCAA 
GGGGCCTCCT TCATTGGGAT CCTGGACATC GCTGGATTTG AGATTTTTGA GCTGAACTCA 
TTCGAGCAAC TGTGCATCAA CTACACCAAC GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAA CTTCATCGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATTGAC CTCATCGAGA GACCTGCTAA CCCTCCTGGC 
GTGCTGGCCC TTCTGGATGA AGAGTGCTGG TTCCCTAAAG CCACAGATAA GACCTTTGTT 
GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC TCCAAGTTTC AGAAACCACG TCAATTGAAA 
GACAAAGCTG ATTTTTGCAT TATCCATTAT GCAGGGAAGG TGGACTACAA GGCAGACGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAT GACAACGTGG CCACCCTCCT GCACCAGTCC 
TCTGACAGAT TCGTGGCAGA GCTCTGGAAG GATGAGATTC AGAATATTCA GAGAGCTTCT 
TTCTATGACA GTGTTTCTGG TCTTCATGAG CCACCAGTGG ACCGGATCGT GGGTCTGGAT 
CAGGTCACGG GGATGACGGA GACGGCATTC GGCTCCGCCT ATAAAACCAA GAAGGGCATG 
TTCCGCACTG TCGGGCAGCT CTACAAAGAA TCGCTCACCA AGCTGATGGC GACGCTCCGG 
AACACCAACC CGAACTTCGT GCGCTGCATC ATCCCAAATC ACGAGAAGCG GGCTGGGAAA 
CTGGACCCAC ACCTGGTGCT GGATCAGCTC CGCTGCAATG GCGTCCTGGA AGGGATCCGC 
ATCTGCCGCC AGGGCTTCCC CAACCGGATC GTTTTCCAGG AATTCAGACA GAGATACGAG 
ATCCTCACTC CAAATGCTAT CCCTAAGGGT TTCATGGATG GCAAACAGGC TTGTGAACGA 
ATGATCCGGG CTTTAGAATT GGACCCAAAC TTGTACAGAA TTGGACAGAG CAAGATATTT 
TTCAGAGCTG GAGTCTTGGC ACACTTAGAG GAAGAACGAG ATTTAAAAAT TACCGACATC 
ATTATCTTCT TCCAGGCTGT TTGCAGGGGT TATTTAGCCA GAAAGGCCTT CGCCAAGAAG 
CAGCAGCAGC TGAGTGCCCT GAAGGTGCTG CAGCGCAACT GTGCCGCGTA CCTGAAGCTG 
CGGCACTGGC AGTGGTGGCG CGTCTTCACC AAGGTGAAAC CACTCCTACA AGTCACTCGT 
CAAGAGGAGG AGCTCCAGGC CAAAGATGAA GAGCTGCTGA AGGTGAAAGA GAAGCAGACG 
AAGGTGGAAG GGGAGCTGGA GGAGATGGAG AGGAAGCACC AACAGCTGCT GGAGGAGAAG 
AATATCCTGG CAGAGCAGCT GCAGGCGGAG ACCGAGCTCT TTGCCGAAGC AGAGGAGATG 
AGGGCCAGAC TTGCCGCTAA GAAGCAGGAA TTAGAAGAGA TTCTCCATGA CTTGGAATCT 
AGGGTAGAAG AAGAAGAAGA AAGAAACCAA ATCCTCCAAA ATGAAAAGAA GAAAATGCAA 
GCACACATTC AGGACCTGGA GGAGCAGCTA GATGAGGAGG AGGGGGCTCG CCAGAAGCTG 
CAGCTGGAGA AGGTGACGGC GGAGGCCAAG ATCAAGAAGA TGGAAGAAGA GATTCTGCTT 
CTGGAGGACC AGAACTCCAA GTTTATCAAA GAAAAGAAAC TCATGGAAGA TCGCATTGCA 
GAATGTTCCT CTCAGCTGGC TGAAGAGGAA GAAAAGGCAA AAAACTTGGC CAAAATCAGG 
AATAAGCAAG AAGTAATGAT CTCGGATTTA GAAGAGCGCT TAAAGAAGGA GGAGAAGACG 
CGCCAGGAGC TGGAGAAGGC CAAAAGGAAA CTGGACGGGG AGACCACGGA CCTGCAAGAC 
CAGATCGCCG AGCTCCAGGC GCAGATTGAC GAGCTTAAGA TCCAGGTGGC CAAGAAAGAG 
GAGGAGCTGC AGGGTGCCCT GGCCAGGGGT GACGACGAGA CGCTACACAA GAACAACGCA 
CTAAAAGTCG TGCGAGAGCT GCAGGCCCAG ATCGCCGAGC TGCAGGAGGA CTTTGAATCT 
GAGAAGGCCT CGCGGAACAA AGCAGAGAAG CAGAAGAGGG ACTTGAGTGA GGAGCTGGAG 
GCACTCAAGA CGGAGCTGGA GGACACGCTG GACACCACGG CGGCCCAGCA GGAACTGCGT 
ACAAAACGAG AACAAGAAGT GGCGGAGCTG AAGAAGGCTC TCGAGGAGGA AACGAAGAGC 
CACGAGGCTC AGATCCAGGA CATGAGGCAG AGGCATGCGA CCGCCTTGGA GGAGCTGTCG 
GAGCAGCTGG AGCAGGCCAA GAGGTTCAAA GCAAATCTGG AAAAGAACAA GCAGGGCCTG 
GAGACAGACA ACAAGGAGCT GGCGTGCGAG GTCAAGGTGC TGCAGCAGGT CAAGGCTGAG 
TCCGAGCACA AGAGGAAGAA GCTGGACGCC CAGGTCCAGG AGCTCCACGC CAAGGTCTCA 
GAGGGAGACA GACTCAGGGT GGAGCTGGCA GAGAAAGCCA ATAAGTTACA GAATGAATTG 
GATAATGTCT CAACTCTTCT AGAAGAAGCA GAGAAGAAGG GTATTAAATT TGCTAAGGAT 
GCAGCTGGTC TGGAGTCTCA GCTCCAAGAC ACACAGGAGC TTCTTCAGGA GGAGACGCGC 
CAGAAACTGA ACCTGAGCAG TCGGATCCGG CAGCTGGAGG AGGAGAGGAG CAGCCTCCAG 
GAGCAGCAGG AGGAAGAGGA GGAGGCCAGG AGGAGCCTGG AGAAGCAGCT GCAGGCCCTG 
CAGGCCCAGT TGACGGATAC CAAGAAGAAA GTAGATGATG ACCTGGGGAC AATTGAAAAT 
TTGGAAGAAG CCAAGAAGAA GCTCCTCAAG GATGTGGAGG TGCTGAGCCA GCGCCTGGAG 
GAGAAGGCCC TGGCCTATGA CAAGCTGGAG AAGACCAAGA CCCGCCTGCA GCAGGAGCTG 
GATGACCTGC TGGTAGACCT GGACCACCAG CGCCAGATCG TCTCCAACTT GGAGAAGAAG 
CAGAAGAAGT TTGATCAGCT GTTAGCGGAA GAGAAGAACA TCTCTGCCCG CTACGCAGAA 
GAGCGCGACC GGGCAGAGGC AGAGGCCCGA GAGAAGGAGA CCAAAGCCCT GTCCCTGGCC 
CGCGCCCTGG AGGAGGCCCT GGAGGCCAGG GAGGAGGCTG AGCGACAGAA TAAGCAGCTG 
CGGGCGGACA TGGAGGACCT CATGAGCTCC AAGGACGATG TGGGCAAGAA CGTTCATGAA 
CTTGAGAAAT CCAAACGGGC CCTAGAGCAG CAGGTGGAGG AAATGAGGAC CCAACTGGAG 
GAGCTGGAAG ATGAGCTCCA GGCTACAGAA GACGCCAAGC TTCGCCTAGA GGTCAACATG 
CAAGCCATGA AAGCCCAGTT TGAGAGAGAC TTGCAGACCA GGGATGAGCA GAATGAGGAA 
AAGAAGCGGC TGCTCATTAA ACAGGTGCGG GAGCTTGAGG CAGAGCTGGA GGATGAGCGG 
AAACAGCGGG CCCTGGCTGT TGCCTCGAAG AAGAAAATGG AAATAGACCT GAAGGACCTG 
GAGGCTCAAA TCGAGGCAGC TAACAAAGCA AGGGATGAAG TGATCAAGCA GCTCCGCAAA 
CTCCAGGCTC AGATGAAGGA CTACCAGCGT GAATTAGAAG AAGCTCGTGC ATCCCGGGAT 
GAGATTTTTG CTCAATCCAA AGAGAGTGAG AAGAAACTGA AAAGCCTGGA AGCAGAAATC 
CTTCAGTTGC AGGAGGAACT TGCCTCATCC GAGCGAGCCC GCCGACATGC TGAGCAGGAG 
AGAGACGAGC TGGCGGACGA GATTGCCAAT AGCGCCTCCG GCAAGTCTGC ACTCCTGGAT 
GAGAAGCGGC GTCTGGAGGC TCGGATCGCA CAGCTAGAGG AGGAGCTGGA GGAGGAGCAG 
AGCAACATGG AGCTGCTCAA TGACCGCTTC CGCAAGACCA CACTGCAGGT GGACACGCTG 
AACACTGAGC TGGCGGCCGA GCGCAGCGCT GCCCAGAAGA GCGATAATGC GCGCCAGCAG 
CTGGAGCGGC AGAACAAGGA GTTGAAGGCC AAGCTGCAGG AGCTGGAAGG CGCTGTCAAG 
TCCAAGTTCA AGGCCACCAT CTCTGCTCTA GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG 
CTTGAGCAGG AAGCCAAGGA AAGAGCCGCT GCCAATAAAC TAGTCCGTCG TACCGAGAAG 
AAGCTGAAAG AAATCTTCAT GCAGGTTGAA GATGAACGCC GCCATGCTGA CCAGTATAAA 
GAACAGATGG AGAAGGCCAA TGCCAGGATG AAGCAGCTCA AGCGGCAGCT GGAGGAAGCA 
GAGGAAGAAG CTACACGTGC CAACGCATCT CGGCGCAAAC TCCAGCGGGA ACTGGATGAT 
GCCACTGAGG CCAACGAGGG CCTGAGCCGC GAGGTCAGCA CCCTGAAGAA CCGGCTGAGG 
CGAGGCGGCC CGATCAGCTT CTCCTCCAGC CGATCAGGCC GGCGCCAGCT GCACATTGAG 
GGCGCGTCCC TGGAGCTGTC GGACGACGAC ACAGAAAGTA AGACCAGTGA CATCAACGAG 
ACACAGCCGC CGCAGTCCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_015458670.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314156.1 Bos taurus myosin, heavy chain 10, non-muscle (MYH10), transcript variant X3, mRNA.

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CloneID	OBa29701
Clone ID Related Accession (Same CDS sequence)	XM_005220297.3 , XM_015458670.1 , XM_005220298.3
Accession Version	XM_015458670.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myosin-10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTCAGGAAT CACCTAAACC AGTGAAACAC 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC ATTTGGAAAC 
GCAAAGACTG TGAAAAATGA TAACTCATCT CGTTTTGGCA AGTTTATTCG GATCAACTTT 
GATGTAACTG GCTATATCGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAATT GTTATCTGGA 
GCAGGAGAAC ATCTGAAGTC TGATTTGCTC CTTGAAGGAT TTAATAACTA CAGATTTCTC 
TCCAATGGCT ATATTCCTAT TCCGGGACAA CAAGACAAAG ATAACTTCCA GGAGACAATG 
GAGGCAATGC ATATAATGGG CTTCTCCCAT GAAGAGATTC TGTCAATGCT TAAAGTGGTA 
TCTTCAGTGC TTCAGTTTGG AAATATTTCT TTCAAAAAGG AGCGAAACAC TGATCAAGCC 
TCCATGCCGG AAAATACAGT TGCACAGAAG CTCTGCCATC TTCTTGGCAT GAATGTGATG 
GAGTTCACTC GGGCCATTCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA CGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCA GTAGAAGCTT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GCTGGCTCGT TCACCGCATC AATAAAGCTC TGGACAGGAC CAAACGGCAA 
GGGGCCTCCT TCATTGGGAT CCTGGACATC GCTGGATTTG AGATTTTTGA GCTGAACTCA 
TTCGAGCAAC TGTGCATCAA CTACACCAAC GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAA CTTCATCGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATTGAC CTCATCGAGA GACCTGCTAA CCCTCCTGGC 
GTGCTGGCCC TTCTGGATGA AGAGTGCTGG TTCCCTAAAG CCACAGATAA GACCTTTGTT 
GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC TCCAAGTTTC AGAAACCACG TCAATTGAAA 
GACAAAGCTG ATTTTTGCAT TATCCATTAT GCAGGGAAGG TGGACTACAA GGCAGACGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAT GACAACGTGG CCACCCTCCT GCACCAGTCC 
TCTGACAGAT TCGTGGCAGA GCTCTGGAAG GATGAGATTC AGAATATTCA GAGAGCTTCT 
TTCTATGACA GTGTTTCTGG TCTTCATGAG CCACCAGTGG ACCGGATCGT GGGTCTGGAT 
CAGGTCACGG GGATGACGGA GACGGCATTC GGCTCCGCCT ATAAAACCAA GAAGGGCATG 
TTCCGCACTG TCGGGCAGCT CTACAAAGAA TCGCTCACCA AGCTGATGGC GACGCTCCGG 
AACACCAACC CGAACTTCGT GCGCTGCATC ATCCCAAATC ACGAGAAGCG GGCTGGGAAA 
CTGGACCCAC ACCTGGTGCT GGATCAGCTC CGCTGCAATG GCGTCCTGGA AGGGATCCGC 
ATCTGCCGCC AGGGCTTCCC CAACCGGATC GTTTTCCAGG AATTCAGACA GAGATACGAG 
ATCCTCACTC CAAATGCTAT CCCTAAGGGT TTCATGGATG GCAAACAGGC TTGTGAACGA 
ATGATCCGGG CTTTAGAATT GGACCCAAAC TTGTACAGAA TTGGACAGAG CAAGATATTT 
TTCAGAGCTG GAGTCTTGGC ACACTTAGAG GAAGAACGAG ATTTAAAAAT TACCGACATC 
ATTATCTTCT TCCAGGCTGT TTGCAGGGGT TATTTAGCCA GAAAGGCCTT CGCCAAGAAG 
CAGCAGCAGC TGAGTGCCCT GAAGGTGCTG CAGCGCAACT GTGCCGCGTA CCTGAAGCTG 
CGGCACTGGC AGTGGTGGCG CGTCTTCACC AAGGTGAAAC CACTCCTACA AGTCACTCGT 
CAAGAGGAGG AGCTCCAGGC CAAAGATGAA GAGCTGCTGA AGGTGAAAGA GAAGCAGACG 
AAGGTGGAAG GGGAGCTGGA GGAGATGGAG AGGAAGCACC AACAGCTGCT GGAGGAGAAG 
AATATCCTGG CAGAGCAGCT GCAGGCGGAG ACCGAGCTCT TTGCCGAAGC AGAGGAGATG 
AGGGCCAGAC TTGCCGCTAA GAAGCAGGAA TTAGAAGAGA TTCTCCATGA CTTGGAATCT 
AGGGTAGAAG AAGAAGAAGA AAGAAACCAA ATCCTCCAAA ATGAAAAGAA GAAAATGCAA 
GCACACATTC AGGACCTGGA GGAGCAGCTA GATGAGGAGG AGGGGGCTCG CCAGAAGCTG 
CAGCTGGAGA AGGTGACGGC GGAGGCCAAG ATCAAGAAGA TGGAAGAAGA GATTCTGCTT 
CTGGAGGACC AGAACTCCAA GTTTATCAAA GAAAAGAAAC TCATGGAAGA TCGCATTGCA 
GAATGTTCCT CTCAGCTGGC TGAAGAGGAA GAAAAGGCAA AAAACTTGGC CAAAATCAGG 
AATAAGCAAG AAGTAATGAT CTCGGATTTA GAAGAGCGCT TAAAGAAGGA GGAGAAGACG 
CGCCAGGAGC TGGAGAAGGC CAAAAGGAAA CTGGACGGGG AGACCACGGA CCTGCAAGAC 
CAGATCGCCG AGCTCCAGGC GCAGATTGAC GAGCTTAAGA TCCAGGTGGC CAAGAAAGAG 
GAGGAGCTGC AGGGTGCCCT GGCCAGGGGT GACGACGAGA CGCTACACAA GAACAACGCA 
CTAAAAGTCG TGCGAGAGCT GCAGGCCCAG ATCGCCGAGC TGCAGGAGGA CTTTGAATCT 
GAGAAGGCCT CGCGGAACAA AGCAGAGAAG CAGAAGAGGG ACTTGAGTGA GGAGCTGGAG 
GCACTCAAGA CGGAGCTGGA GGACACGCTG GACACCACGG CGGCCCAGCA GGAACTGCGT 
ACAAAACGAG AACAAGAAGT GGCGGAGCTG AAGAAGGCTC TCGAGGAGGA AACGAAGAGC 
CACGAGGCTC AGATCCAGGA CATGAGGCAG AGGCATGCGA CCGCCTTGGA GGAGCTGTCG 
GAGCAGCTGG AGCAGGCCAA GAGGTTCAAA GCAAATCTGG AAAAGAACAA GCAGGGCCTG 
GAGACAGACA ACAAGGAGCT GGCGTGCGAG GTCAAGGTGC TGCAGCAGGT CAAGGCTGAG 
TCCGAGCACA AGAGGAAGAA GCTGGACGCC CAGGTCCAGG AGCTCCACGC CAAGGTCTCA 
GAGGGAGACA GACTCAGGGT GGAGCTGGCA GAGAAAGCCA ATAAGTTACA GAATGAATTG 
GATAATGTCT CAACTCTTCT AGAAGAAGCA GAGAAGAAGG GTATTAAATT TGCTAAGGAT 
GCAGCTGGTC TGGAGTCTCA GCTCCAAGAC ACACAGGAGC TTCTTCAGGA GGAGACGCGC 
CAGAAACTGA ACCTGAGCAG TCGGATCCGG CAGCTGGAGG AGGAGAGGAG CAGCCTCCAG 
GAGCAGCAGG AGGAAGAGGA GGAGGCCAGG AGGAGCCTGG AGAAGCAGCT GCAGGCCCTG 
CAGGCCCAGT TGACGGATAC CAAGAAGAAA GTAGATGATG ACCTGGGGAC AATTGAAAAT 
TTGGAAGAAG CCAAGAAGAA GCTCCTCAAG GATGTGGAGG TGCTGAGCCA GCGCCTGGAG 
GAGAAGGCCC TGGCCTATGA CAAGCTGGAG AAGACCAAGA CCCGCCTGCA GCAGGAGCTG 
GATGACCTGC TGGTAGACCT GGACCACCAG CGCCAGATCG TCTCCAACTT GGAGAAGAAG 
CAGAAGAAGT TTGATCAGCT GTTAGCGGAA GAGAAGAACA TCTCTGCCCG CTACGCAGAA 
GAGCGCGACC GGGCAGAGGC AGAGGCCCGA GAGAAGGAGA CCAAAGCCCT GTCCCTGGCC 
CGCGCCCTGG AGGAGGCCCT GGAGGCCAGG GAGGAGGCTG AGCGACAGAA TAAGCAGCTG 
CGGGCGGACA TGGAGGACCT CATGAGCTCC AAGGACGATG TGGGCAAGAA CGTTCATGAA 
CTTGAGAAAT CCAAACGGGC CCTAGAGCAG CAGGTGGAGG AAATGAGGAC CCAACTGGAG 
GAGCTGGAAG ATGAGCTCCA GGCTACAGAA GACGCCAAGC TTCGCCTAGA GGTCAACATG 
CAAGCCATGA AAGCCCAGTT TGAGAGAGAC TTGCAGACCA GGGATGAGCA GAATGAGGAA 
AAGAAGCGGC TGCTCATTAA ACAGGTGCGG GAGCTTGAGG CAGAGCTGGA GGATGAGCGG 
AAACAGCGGG CCCTGGCTGT TGCCTCGAAG AAGAAAATGG AAATAGACCT GAAGGACCTG 
GAGGCTCAAA TCGAGGCAGC TAACAAAGCA AGGGATGAAG TGATCAAGCA GCTCCGCAAA 
CTCCAGGCTC AGATGAAGGA CTACCAGCGT GAATTAGAAG AAGCTCGTGC ATCCCGGGAT 
GAGATTTTTG CTCAATCCAA AGAGAGTGAG AAGAAACTGA AAAGCCTGGA AGCAGAAATC 
CTTCAGTTGC AGGAGGAACT TGCCTCATCC GAGCGAGCCC GCCGACATGC TGAGCAGGAG 
AGAGACGAGC TGGCGGACGA GATTGCCAAT AGCGCCTCCG GCAAGTCTGC ACTCCTGGAT 
GAGAAGCGGC GTCTGGAGGC TCGGATCGCA CAGCTAGAGG AGGAGCTGGA GGAGGAGCAG 
AGCAACATGG AGCTGCTCAA TGACCGCTTC CGCAAGACCA CACTGCAGGT GGACACGCTG 
AACACTGAGC TGGCGGCCGA GCGCAGCGCT GCCCAGAAGA GCGATAATGC GCGCCAGCAG 
CTGGAGCGGC AGAACAAGGA GTTGAAGGCC AAGCTGCAGG AGCTGGAAGG CGCTGTCAAG 
TCCAAGTTCA AGGCCACCAT CTCTGCTCTA GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG 
CTTGAGCAGG AAGCCAAGGA AAGAGCCGCT GCCAATAAAC TAGTCCGTCG TACCGAGAAG 
AAGCTGAAAG AAATCTTCAT GCAGGTTGAA GATGAACGCC GCCATGCTGA CCAGTATAAA 
GAACAGATGG AGAAGGCCAA TGCCAGGATG AAGCAGCTCA AGCGGCAGCT GGAGGAAGCA 
GAGGAAGAAG CTACACGTGC CAACGCATCT CGGCGCAAAC TCCAGCGGGA ACTGGATGAT 
GCCACTGAGG CCAACGAGGG CCTGAGCCGC GAGGTCAGCA CCCTGAAGAA CCGGCTGAGG 
CGAGGCGGCC CGATCAGCTT CTCCTCCAGC CGATCAGGCC GGCGCCAGCT GCACATTGAG 
GGCGCGTCCC TGGAGCTGTC GGACGACGAC ACAGAAAGTA AGACCAGTGA CATCAACGAG 
ACACAGCCGC CGCAGTCCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314156.1
CDS	1..6024
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVLVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPQESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDEIQNIQRASFYDSVSGLHEPPVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKEKQTKVEGELEEMERKHQQLLEEKNILAEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKIQVAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETKSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSTLLEEAEKKGIKFAKDAAGLESQLQDTQELLQEETRQKLNLSSRIRQLEEERSSLQEQQEEEEEARRSLEKQLQALQAQLTDTKKKVDDDLGTIENLEEAKKKLLKDVEVLSQRLEEKALAYDKLEKTKTRLQQELDDLLVDLDHQRQIVSNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALEAREEAERQNKQLRADMEDLMSSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLIKQVRELEAELEDERKQRALAVASKKKMEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNTELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDINETQPPQSE

Target ORF information:

RefSeq Version	XM_015458670.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin, heavy chain 10, non-muscle (MYH10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458670.1

ORF Insert Sequence:

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTCAGGAAT CACCTAAACC AGTGAAACAC 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC ATTTGGAAAC 
GCAAAGACTG TGAAAAATGA TAACTCATCT CGTTTTGGCA AGTTTATTCG GATCAACTTT 
GATGTAACTG GCTATATCGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAATT GTTATCTGGA 
GCAGGAGAAC ATCTGAAGTC TGATTTGCTC CTTGAAGGAT TTAATAACTA CAGATTTCTC 
TCCAATGGCT ATATTCCTAT TCCGGGACAA CAAGACAAAG ATAACTTCCA GGAGACAATG 
GAGGCAATGC ATATAATGGG CTTCTCCCAT GAAGAGATTC TGTCAATGCT TAAAGTGGTA 
TCTTCAGTGC TTCAGTTTGG AAATATTTCT TTCAAAAAGG AGCGAAACAC TGATCAAGCC 
TCCATGCCGG AAAATACAGT TGCACAGAAG CTCTGCCATC TTCTTGGCAT GAATGTGATG 
GAGTTCACTC GGGCCATTCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA CGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCA GTAGAAGCTT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GCTGGCTCGT TCACCGCATC AATAAAGCTC TGGACAGGAC CAAACGGCAA 
GGGGCCTCCT TCATTGGGAT CCTGGACATC GCTGGATTTG AGATTTTTGA GCTGAACTCA 
TTCGAGCAAC TGTGCATCAA CTACACCAAC GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAA CTTCATCGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATTGAC CTCATCGAGA GACCTGCTAA CCCTCCTGGC 
GTGCTGGCCC TTCTGGATGA AGAGTGCTGG TTCCCTAAAG CCACAGATAA GACCTTTGTT 
GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC TCCAAGTTTC AGAAACCACG TCAATTGAAA 
GACAAAGCTG ATTTTTGCAT TATCCATTAT GCAGGGAAGG TGGACTACAA GGCAGACGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAT GACAACGTGG CCACCCTCCT GCACCAGTCC 
TCTGACAGAT TCGTGGCAGA GCTCTGGAAG GATGAGATTC AGAATATTCA GAGAGCTTCT 
TTCTATGACA GTGTTTCTGG TCTTCATGAG CCACCAGTGG ACCGGATCGT GGGTCTGGAT 
CAGGTCACGG GGATGACGGA GACGGCATTC GGCTCCGCCT ATAAAACCAA GAAGGGCATG 
TTCCGCACTG TCGGGCAGCT CTACAAAGAA TCGCTCACCA AGCTGATGGC GACGCTCCGG 
AACACCAACC CGAACTTCGT GCGCTGCATC ATCCCAAATC ACGAGAAGCG GGCTGGGAAA 
CTGGACCCAC ACCTGGTGCT GGATCAGCTC CGCTGCAATG GCGTCCTGGA AGGGATCCGC 
ATCTGCCGCC AGGGCTTCCC CAACCGGATC GTTTTCCAGG AATTCAGACA GAGATACGAG 
ATCCTCACTC CAAATGCTAT CCCTAAGGGT TTCATGGATG GCAAACAGGC TTGTGAACGA 
ATGATCCGGG CTTTAGAATT GGACCCAAAC TTGTACAGAA TTGGACAGAG CAAGATATTT 
TTCAGAGCTG GAGTCTTGGC ACACTTAGAG GAAGAACGAG ATTTAAAAAT TACCGACATC 
ATTATCTTCT TCCAGGCTGT TTGCAGGGGT TATTTAGCCA GAAAGGCCTT CGCCAAGAAG 
CAGCAGCAGC TGAGTGCCCT GAAGGTGCTG CAGCGCAACT GTGCCGCGTA CCTGAAGCTG 
CGGCACTGGC AGTGGTGGCG CGTCTTCACC AAGGTGAAAC CACTCCTACA AGTCACTCGT 
CAAGAGGAGG AGCTCCAGGC CAAAGATGAA GAGCTGCTGA AGGTGAAAGA GAAGCAGACG 
AAGGTGGAAG GGGAGCTGGA GGAGATGGAG AGGAAGCACC AACAGCTGCT GGAGGAGAAG 
AATATCCTGG CAGAGCAGCT GCAGGCGGAG ACCGAGCTCT TTGCCGAAGC AGAGGAGATG 
AGGGCCAGAC TTGCCGCTAA GAAGCAGGAA TTAGAAGAGA TTCTCCATGA CTTGGAATCT 
AGGGTAGAAG AAGAAGAAGA AAGAAACCAA ATCCTCCAAA ATGAAAAGAA GAAAATGCAA 
GCACACATTC AGGACCTGGA GGAGCAGCTA GATGAGGAGG AGGGGGCTCG CCAGAAGCTG 
CAGCTGGAGA AGGTGACGGC GGAGGCCAAG ATCAAGAAGA TGGAAGAAGA GATTCTGCTT 
CTGGAGGACC AGAACTCCAA GTTTATCAAA GAAAAGAAAC TCATGGAAGA TCGCATTGCA 
GAATGTTCCT CTCAGCTGGC TGAAGAGGAA GAAAAGGCAA AAAACTTGGC CAAAATCAGG 
AATAAGCAAG AAGTAATGAT CTCGGATTTA GAAGAGCGCT TAAAGAAGGA GGAGAAGACG 
CGCCAGGAGC TGGAGAAGGC CAAAAGGAAA CTGGACGGGG AGACCACGGA CCTGCAAGAC 
CAGATCGCCG AGCTCCAGGC GCAGATTGAC GAGCTTAAGA TCCAGGTGGC CAAGAAAGAG 
GAGGAGCTGC AGGGTGCCCT GGCCAGGGGT GACGACGAGA CGCTACACAA GAACAACGCA 
CTAAAAGTCG TGCGAGAGCT GCAGGCCCAG ATCGCCGAGC TGCAGGAGGA CTTTGAATCT 
GAGAAGGCCT CGCGGAACAA AGCAGAGAAG CAGAAGAGGG ACTTGAGTGA GGAGCTGGAG 
GCACTCAAGA CGGAGCTGGA GGACACGCTG GACACCACGG CGGCCCAGCA GGAACTGCGT 
ACAAAACGAG AACAAGAAGT GGCGGAGCTG AAGAAGGCTC TCGAGGAGGA AACGAAGAGC 
CACGAGGCTC AGATCCAGGA CATGAGGCAG AGGCATGCGA CCGCCTTGGA GGAGCTGTCG 
GAGCAGCTGG AGCAGGCCAA GAGGTTCAAA GCAAATCTGG AAAAGAACAA GCAGGGCCTG 
GAGACAGACA ACAAGGAGCT GGCGTGCGAG GTCAAGGTGC TGCAGCAGGT CAAGGCTGAG 
TCCGAGCACA AGAGGAAGAA GCTGGACGCC CAGGTCCAGG AGCTCCACGC CAAGGTCTCA 
GAGGGAGACA GACTCAGGGT GGAGCTGGCA GAGAAAGCCA ATAAGTTACA GAATGAATTG 
GATAATGTCT CAACTCTTCT AGAAGAAGCA GAGAAGAAGG GTATTAAATT TGCTAAGGAT 
GCAGCTGGTC TGGAGTCTCA GCTCCAAGAC ACACAGGAGC TTCTTCAGGA GGAGACGCGC 
CAGAAACTGA ACCTGAGCAG TCGGATCCGG CAGCTGGAGG AGGAGAGGAG CAGCCTCCAG 
GAGCAGCAGG AGGAAGAGGA GGAGGCCAGG AGGAGCCTGG AGAAGCAGCT GCAGGCCCTG 
CAGGCCCAGT TGACGGATAC CAAGAAGAAA GTAGATGATG ACCTGGGGAC AATTGAAAAT 
TTGGAAGAAG CCAAGAAGAA GCTCCTCAAG GATGTGGAGG TGCTGAGCCA GCGCCTGGAG 
GAGAAGGCCC TGGCCTATGA CAAGCTGGAG AAGACCAAGA CCCGCCTGCA GCAGGAGCTG 
GATGACCTGC TGGTAGACCT GGACCACCAG CGCCAGATCG TCTCCAACTT GGAGAAGAAG 
CAGAAGAAGT TTGATCAGCT GTTAGCGGAA GAGAAGAACA TCTCTGCCCG CTACGCAGAA 
GAGCGCGACC GGGCAGAGGC AGAGGCCCGA GAGAAGGAGA CCAAAGCCCT GTCCCTGGCC 
CGCGCCCTGG AGGAGGCCCT GGAGGCCAGG GAGGAGGCTG AGCGACAGAA TAAGCAGCTG 
CGGGCGGACA TGGAGGACCT CATGAGCTCC AAGGACGATG TGGGCAAGAA CGTTCATGAA 
CTTGAGAAAT CCAAACGGGC CCTAGAGCAG CAGGTGGAGG AAATGAGGAC CCAACTGGAG 
GAGCTGGAAG ATGAGCTCCA GGCTACAGAA GACGCCAAGC TTCGCCTAGA GGTCAACATG 
CAAGCCATGA AAGCCCAGTT TGAGAGAGAC TTGCAGACCA GGGATGAGCA GAATGAGGAA 
AAGAAGCGGC TGCTCATTAA ACAGGTGCGG GAGCTTGAGG CAGAGCTGGA GGATGAGCGG 
AAACAGCGGG CCCTGGCTGT TGCCTCGAAG AAGAAAATGG AAATAGACCT GAAGGACCTG 
GAGGCTCAAA TCGAGGCAGC TAACAAAGCA AGGGATGAAG TGATCAAGCA GCTCCGCAAA 
CTCCAGGCTC AGATGAAGGA CTACCAGCGT GAATTAGAAG AAGCTCGTGC ATCCCGGGAT 
GAGATTTTTG CTCAATCCAA AGAGAGTGAG AAGAAACTGA AAAGCCTGGA AGCAGAAATC 
CTTCAGTTGC AGGAGGAACT TGCCTCATCC GAGCGAGCCC GCCGACATGC TGAGCAGGAG 
AGAGACGAGC TGGCGGACGA GATTGCCAAT AGCGCCTCCG GCAAGTCTGC ACTCCTGGAT 
GAGAAGCGGC GTCTGGAGGC TCGGATCGCA CAGCTAGAGG AGGAGCTGGA GGAGGAGCAG 
AGCAACATGG AGCTGCTCAA TGACCGCTTC CGCAAGACCA CACTGCAGGT GGACACGCTG 
AACACTGAGC TGGCGGCCGA GCGCAGCGCT GCCCAGAAGA GCGATAATGC GCGCCAGCAG 
CTGGAGCGGC AGAACAAGGA GTTGAAGGCC AAGCTGCAGG AGCTGGAAGG CGCTGTCAAG 
TCCAAGTTCA AGGCCACCAT CTCTGCTCTA GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG 
CTTGAGCAGG AAGCCAAGGA AAGAGCCGCT GCCAATAAAC TAGTCCGTCG TACCGAGAAG 
AAGCTGAAAG AAATCTTCAT GCAGGTTGAA GATGAACGCC GCCATGCTGA CCAGTATAAA 
GAACAGATGG AGAAGGCCAA TGCCAGGATG AAGCAGCTCA AGCGGCAGCT GGAGGAAGCA 
GAGGAAGAAG CTACACGTGC CAACGCATCT CGGCGCAAAC TCCAGCGGGA ACTGGATGAT 
GCCACTGAGG CCAACGAGGG CCTGAGCCGC GAGGTCAGCA CCCTGAAGAA CCGGCTGAGG 
CGAGGCGGCC CGATCAGCTT CTCCTCCAGC CGATCAGGCC GGCGCCAGCT GCACATTGAG 
GGCGCGTCCC TGGAGCTGTC GGACGACGAC ACAGAAAGTA AGACCAGTGA CATCAACGAG 
ACACAGCCGC CGCAGTCCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_005220298.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220355.1 Bos taurus myosin, heavy chain 10, non-muscle (MYH10), transcript variant X2, mRNA.

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CloneID	OBa29701
Clone ID Related Accession (Same CDS sequence)	XM_005220297.3 , XM_015458670.1 , XM_005220298.3
Accession Version	XM_005220298.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myosin-10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220298.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTCAGGAAT CACCTAAACC AGTGAAACAC 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC ATTTGGAAAC 
GCAAAGACTG TGAAAAATGA TAACTCATCT CGTTTTGGCA AGTTTATTCG GATCAACTTT 
GATGTAACTG GCTATATCGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAATT GTTATCTGGA 
GCAGGAGAAC ATCTGAAGTC TGATTTGCTC CTTGAAGGAT TTAATAACTA CAGATTTCTC 
TCCAATGGCT ATATTCCTAT TCCGGGACAA CAAGACAAAG ATAACTTCCA GGAGACAATG 
GAGGCAATGC ATATAATGGG CTTCTCCCAT GAAGAGATTC TGTCAATGCT TAAAGTGGTA 
TCTTCAGTGC TTCAGTTTGG AAATATTTCT TTCAAAAAGG AGCGAAACAC TGATCAAGCC 
TCCATGCCGG AAAATACAGT TGCACAGAAG CTCTGCCATC TTCTTGGCAT GAATGTGATG 
GAGTTCACTC GGGCCATTCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA CGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCA GTAGAAGCTT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GCTGGCTCGT TCACCGCATC AATAAAGCTC TGGACAGGAC CAAACGGCAA 
GGGGCCTCCT TCATTGGGAT CCTGGACATC GCTGGATTTG AGATTTTTGA GCTGAACTCA 
TTCGAGCAAC TGTGCATCAA CTACACCAAC GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAA CTTCATCGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATTGAC CTCATCGAGA GACCTGCTAA CCCTCCTGGC 
GTGCTGGCCC TTCTGGATGA AGAGTGCTGG TTCCCTAAAG CCACAGATAA GACCTTTGTT 
GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC TCCAAGTTTC AGAAACCACG TCAATTGAAA 
GACAAAGCTG ATTTTTGCAT TATCCATTAT GCAGGGAAGG TGGACTACAA GGCAGACGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAT GACAACGTGG CCACCCTCCT GCACCAGTCC 
TCTGACAGAT TCGTGGCAGA GCTCTGGAAG GATGAGATTC AGAATATTCA GAGAGCTTCT 
TTCTATGACA GTGTTTCTGG TCTTCATGAG CCACCAGTGG ACCGGATCGT GGGTCTGGAT 
CAGGTCACGG GGATGACGGA GACGGCATTC GGCTCCGCCT ATAAAACCAA GAAGGGCATG 
TTCCGCACTG TCGGGCAGCT CTACAAAGAA TCGCTCACCA AGCTGATGGC GACGCTCCGG 
AACACCAACC CGAACTTCGT GCGCTGCATC ATCCCAAATC ACGAGAAGCG GGCTGGGAAA 
CTGGACCCAC ACCTGGTGCT GGATCAGCTC CGCTGCAATG GCGTCCTGGA AGGGATCCGC 
ATCTGCCGCC AGGGCTTCCC CAACCGGATC GTTTTCCAGG AATTCAGACA GAGATACGAG 
ATCCTCACTC CAAATGCTAT CCCTAAGGGT TTCATGGATG GCAAACAGGC TTGTGAACGA 
ATGATCCGGG CTTTAGAATT GGACCCAAAC TTGTACAGAA TTGGACAGAG CAAGATATTT 
TTCAGAGCTG GAGTCTTGGC ACACTTAGAG GAAGAACGAG ATTTAAAAAT TACCGACATC 
ATTATCTTCT TCCAGGCTGT TTGCAGGGGT TATTTAGCCA GAAAGGCCTT CGCCAAGAAG 
CAGCAGCAGC TGAGTGCCCT GAAGGTGCTG CAGCGCAACT GTGCCGCGTA CCTGAAGCTG 
CGGCACTGGC AGTGGTGGCG CGTCTTCACC AAGGTGAAAC CACTCCTACA AGTCACTCGT 
CAAGAGGAGG AGCTCCAGGC CAAAGATGAA GAGCTGCTGA AGGTGAAAGA GAAGCAGACG 
AAGGTGGAAG GGGAGCTGGA GGAGATGGAG AGGAAGCACC AACAGCTGCT GGAGGAGAAG 
AATATCCTGG CAGAGCAGCT GCAGGCGGAG ACCGAGCTCT TTGCCGAAGC AGAGGAGATG 
AGGGCCAGAC TTGCCGCTAA GAAGCAGGAA TTAGAAGAGA TTCTCCATGA CTTGGAATCT 
AGGGTAGAAG AAGAAGAAGA AAGAAACCAA ATCCTCCAAA ATGAAAAGAA GAAAATGCAA 
GCACACATTC AGGACCTGGA GGAGCAGCTA GATGAGGAGG AGGGGGCTCG CCAGAAGCTG 
CAGCTGGAGA AGGTGACGGC GGAGGCCAAG ATCAAGAAGA TGGAAGAAGA GATTCTGCTT 
CTGGAGGACC AGAACTCCAA GTTTATCAAA GAAAAGAAAC TCATGGAAGA TCGCATTGCA 
GAATGTTCCT CTCAGCTGGC TGAAGAGGAA GAAAAGGCAA AAAACTTGGC CAAAATCAGG 
AATAAGCAAG AAGTAATGAT CTCGGATTTA GAAGAGCGCT TAAAGAAGGA GGAGAAGACG 
CGCCAGGAGC TGGAGAAGGC CAAAAGGAAA CTGGACGGGG AGACCACGGA CCTGCAAGAC 
CAGATCGCCG AGCTCCAGGC GCAGATTGAC GAGCTTAAGA TCCAGGTGGC CAAGAAAGAG 
GAGGAGCTGC AGGGTGCCCT GGCCAGGGGT GACGACGAGA CGCTACACAA GAACAACGCA 
CTAAAAGTCG TGCGAGAGCT GCAGGCCCAG ATCGCCGAGC TGCAGGAGGA CTTTGAATCT 
GAGAAGGCCT CGCGGAACAA AGCAGAGAAG CAGAAGAGGG ACTTGAGTGA GGAGCTGGAG 
GCACTCAAGA CGGAGCTGGA GGACACGCTG GACACCACGG CGGCCCAGCA GGAACTGCGT 
ACAAAACGAG AACAAGAAGT GGCGGAGCTG AAGAAGGCTC TCGAGGAGGA AACGAAGAGC 
CACGAGGCTC AGATCCAGGA CATGAGGCAG AGGCATGCGA CCGCCTTGGA GGAGCTGTCG 
GAGCAGCTGG AGCAGGCCAA GAGGTTCAAA GCAAATCTGG AAAAGAACAA GCAGGGCCTG 
GAGACAGACA ACAAGGAGCT GGCGTGCGAG GTCAAGGTGC TGCAGCAGGT CAAGGCTGAG 
TCCGAGCACA AGAGGAAGAA GCTGGACGCC CAGGTCCAGG AGCTCCACGC CAAGGTCTCA 
GAGGGAGACA GACTCAGGGT GGAGCTGGCA GAGAAAGCCA ATAAGTTACA GAATGAATTG 
GATAATGTCT CAACTCTTCT AGAAGAAGCA GAGAAGAAGG GTATTAAATT TGCTAAGGAT 
GCAGCTGGTC TGGAGTCTCA GCTCCAAGAC ACACAGGAGC TTCTTCAGGA GGAGACGCGC 
CAGAAACTGA ACCTGAGCAG TCGGATCCGG CAGCTGGAGG AGGAGAGGAG CAGCCTCCAG 
GAGCAGCAGG AGGAAGAGGA GGAGGCCAGG AGGAGCCTGG AGAAGCAGCT GCAGGCCCTG 
CAGGCCCAGT TGACGGATAC CAAGAAGAAA GTAGATGATG ACCTGGGGAC AATTGAAAAT 
TTGGAAGAAG CCAAGAAGAA GCTCCTCAAG GATGTGGAGG TGCTGAGCCA GCGCCTGGAG 
GAGAAGGCCC TGGCCTATGA CAAGCTGGAG AAGACCAAGA CCCGCCTGCA GCAGGAGCTG 
GATGACCTGC TGGTAGACCT GGACCACCAG CGCCAGATCG TCTCCAACTT GGAGAAGAAG 
CAGAAGAAGT TTGATCAGCT GTTAGCGGAA GAGAAGAACA TCTCTGCCCG CTACGCAGAA 
GAGCGCGACC GGGCAGAGGC AGAGGCCCGA GAGAAGGAGA CCAAAGCCCT GTCCCTGGCC 
CGCGCCCTGG AGGAGGCCCT GGAGGCCAGG GAGGAGGCTG AGCGACAGAA TAAGCAGCTG 
CGGGCGGACA TGGAGGACCT CATGAGCTCC AAGGACGATG TGGGCAAGAA CGTTCATGAA 
CTTGAGAAAT CCAAACGGGC CCTAGAGCAG CAGGTGGAGG AAATGAGGAC CCAACTGGAG 
GAGCTGGAAG ATGAGCTCCA GGCTACAGAA GACGCCAAGC TTCGCCTAGA GGTCAACATG 
CAAGCCATGA AAGCCCAGTT TGAGAGAGAC TTGCAGACCA GGGATGAGCA GAATGAGGAA 
AAGAAGCGGC TGCTCATTAA ACAGGTGCGG GAGCTTGAGG CAGAGCTGGA GGATGAGCGG 
AAACAGCGGG CCCTGGCTGT TGCCTCGAAG AAGAAAATGG AAATAGACCT GAAGGACCTG 
GAGGCTCAAA TCGAGGCAGC TAACAAAGCA AGGGATGAAG TGATCAAGCA GCTCCGCAAA 
CTCCAGGCTC AGATGAAGGA CTACCAGCGT GAATTAGAAG AAGCTCGTGC ATCCCGGGAT 
GAGATTTTTG CTCAATCCAA AGAGAGTGAG AAGAAACTGA AAAGCCTGGA AGCAGAAATC 
CTTCAGTTGC AGGAGGAACT TGCCTCATCC GAGCGAGCCC GCCGACATGC TGAGCAGGAG 
AGAGACGAGC TGGCGGACGA GATTGCCAAT AGCGCCTCCG GCAAGTCTGC ACTCCTGGAT 
GAGAAGCGGC GTCTGGAGGC TCGGATCGCA CAGCTAGAGG AGGAGCTGGA GGAGGAGCAG 
AGCAACATGG AGCTGCTCAA TGACCGCTTC CGCAAGACCA CACTGCAGGT GGACACGCTG 
AACACTGAGC TGGCGGCCGA GCGCAGCGCT GCCCAGAAGA GCGATAATGC GCGCCAGCAG 
CTGGAGCGGC AGAACAAGGA GTTGAAGGCC AAGCTGCAGG AGCTGGAAGG CGCTGTCAAG 
TCCAAGTTCA AGGCCACCAT CTCTGCTCTA GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG 
CTTGAGCAGG AAGCCAAGGA AAGAGCCGCT GCCAATAAAC TAGTCCGTCG TACCGAGAAG 
AAGCTGAAAG AAATCTTCAT GCAGGTTGAA GATGAACGCC GCCATGCTGA CCAGTATAAA 
GAACAGATGG AGAAGGCCAA TGCCAGGATG AAGCAGCTCA AGCGGCAGCT GGAGGAAGCA 
GAGGAAGAAG CTACACGTGC CAACGCATCT CGGCGCAAAC TCCAGCGGGA ACTGGATGAT 
GCCACTGAGG CCAACGAGGG CCTGAGCCGC GAGGTCAGCA CCCTGAAGAA CCGGCTGAGG 
CGAGGCGGCC CGATCAGCTT CTCCTCCAGC CGATCAGGCC GGCGCCAGCT GCACATTGAG 
GGCGCGTCCC TGGAGCTGTC GGACGACGAC ACAGAAAGTA AGACCAGTGA CATCAACGAG 
ACACAGCCGC CGCAGTCCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220355.1
CDS	143..6166
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVLVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPQESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDEIQNIQRASFYDSVSGLHEPPVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKEKQTKVEGELEEMERKHQQLLEEKNILAEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKIQVAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETKSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSTLLEEAEKKGIKFAKDAAGLESQLQDTQELLQEETRQKLNLSSRIRQLEEERSSLQEQQEEEEEARRSLEKQLQALQAQLTDTKKKVDDDLGTIENLEEAKKKLLKDVEVLSQRLEEKALAYDKLEKTKTRLQQELDDLLVDLDHQRQIVSNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALEAREEAERQNKQLRADMEDLMSSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLIKQVRELEAELEDERKQRALAVASKKKMEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNTELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDINETQPPQSE

Target ORF information:

RefSeq Version	XM_005220298.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin, heavy chain 10, non-muscle (MYH10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220298.3

ORF Insert Sequence:

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTCAGGAAT CACCTAAACC AGTGAAACAC 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC ATTTGGAAAC 
GCAAAGACTG TGAAAAATGA TAACTCATCT CGTTTTGGCA AGTTTATTCG GATCAACTTT 
GATGTAACTG GCTATATCGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAATT GTTATCTGGA 
GCAGGAGAAC ATCTGAAGTC TGATTTGCTC CTTGAAGGAT TTAATAACTA CAGATTTCTC 
TCCAATGGCT ATATTCCTAT TCCGGGACAA CAAGACAAAG ATAACTTCCA GGAGACAATG 
GAGGCAATGC ATATAATGGG CTTCTCCCAT GAAGAGATTC TGTCAATGCT TAAAGTGGTA 
TCTTCAGTGC TTCAGTTTGG AAATATTTCT TTCAAAAAGG AGCGAAACAC TGATCAAGCC 
TCCATGCCGG AAAATACAGT TGCACAGAAG CTCTGCCATC TTCTTGGCAT GAATGTGATG 
GAGTTCACTC GGGCCATTCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA CGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCA GTAGAAGCTT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GCTGGCTCGT TCACCGCATC AATAAAGCTC TGGACAGGAC CAAACGGCAA 
GGGGCCTCCT TCATTGGGAT CCTGGACATC GCTGGATTTG AGATTTTTGA GCTGAACTCA 
TTCGAGCAAC TGTGCATCAA CTACACCAAC GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAA CTTCATCGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATTGAC CTCATCGAGA GACCTGCTAA CCCTCCTGGC 
GTGCTGGCCC TTCTGGATGA AGAGTGCTGG TTCCCTAAAG CCACAGATAA GACCTTTGTT 
GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC TCCAAGTTTC AGAAACCACG TCAATTGAAA 
GACAAAGCTG ATTTTTGCAT TATCCATTAT GCAGGGAAGG TGGACTACAA GGCAGACGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAT GACAACGTGG CCACCCTCCT GCACCAGTCC 
TCTGACAGAT TCGTGGCAGA GCTCTGGAAG GATGAGATTC AGAATATTCA GAGAGCTTCT 
TTCTATGACA GTGTTTCTGG TCTTCATGAG CCACCAGTGG ACCGGATCGT GGGTCTGGAT 
CAGGTCACGG GGATGACGGA GACGGCATTC GGCTCCGCCT ATAAAACCAA GAAGGGCATG 
TTCCGCACTG TCGGGCAGCT CTACAAAGAA TCGCTCACCA AGCTGATGGC GACGCTCCGG 
AACACCAACC CGAACTTCGT GCGCTGCATC ATCCCAAATC ACGAGAAGCG GGCTGGGAAA 
CTGGACCCAC ACCTGGTGCT GGATCAGCTC CGCTGCAATG GCGTCCTGGA AGGGATCCGC 
ATCTGCCGCC AGGGCTTCCC CAACCGGATC GTTTTCCAGG AATTCAGACA GAGATACGAG 
ATCCTCACTC CAAATGCTAT CCCTAAGGGT TTCATGGATG GCAAACAGGC TTGTGAACGA 
ATGATCCGGG CTTTAGAATT GGACCCAAAC TTGTACAGAA TTGGACAGAG CAAGATATTT 
TTCAGAGCTG GAGTCTTGGC ACACTTAGAG GAAGAACGAG ATTTAAAAAT TACCGACATC 
ATTATCTTCT TCCAGGCTGT TTGCAGGGGT TATTTAGCCA GAAAGGCCTT CGCCAAGAAG 
CAGCAGCAGC TGAGTGCCCT GAAGGTGCTG CAGCGCAACT GTGCCGCGTA CCTGAAGCTG 
CGGCACTGGC AGTGGTGGCG CGTCTTCACC AAGGTGAAAC CACTCCTACA AGTCACTCGT 
CAAGAGGAGG AGCTCCAGGC CAAAGATGAA GAGCTGCTGA AGGTGAAAGA GAAGCAGACG 
AAGGTGGAAG GGGAGCTGGA GGAGATGGAG AGGAAGCACC AACAGCTGCT GGAGGAGAAG 
AATATCCTGG CAGAGCAGCT GCAGGCGGAG ACCGAGCTCT TTGCCGAAGC AGAGGAGATG 
AGGGCCAGAC TTGCCGCTAA GAAGCAGGAA TTAGAAGAGA TTCTCCATGA CTTGGAATCT 
AGGGTAGAAG AAGAAGAAGA AAGAAACCAA ATCCTCCAAA ATGAAAAGAA GAAAATGCAA 
GCACACATTC AGGACCTGGA GGAGCAGCTA GATGAGGAGG AGGGGGCTCG CCAGAAGCTG 
CAGCTGGAGA AGGTGACGGC GGAGGCCAAG ATCAAGAAGA TGGAAGAAGA GATTCTGCTT 
CTGGAGGACC AGAACTCCAA GTTTATCAAA GAAAAGAAAC TCATGGAAGA TCGCATTGCA 
GAATGTTCCT CTCAGCTGGC TGAAGAGGAA GAAAAGGCAA AAAACTTGGC CAAAATCAGG 
AATAAGCAAG AAGTAATGAT CTCGGATTTA GAAGAGCGCT TAAAGAAGGA GGAGAAGACG 
CGCCAGGAGC TGGAGAAGGC CAAAAGGAAA CTGGACGGGG AGACCACGGA CCTGCAAGAC 
CAGATCGCCG AGCTCCAGGC GCAGATTGAC GAGCTTAAGA TCCAGGTGGC CAAGAAAGAG 
GAGGAGCTGC AGGGTGCCCT GGCCAGGGGT GACGACGAGA CGCTACACAA GAACAACGCA 
CTAAAAGTCG TGCGAGAGCT GCAGGCCCAG ATCGCCGAGC TGCAGGAGGA CTTTGAATCT 
GAGAAGGCCT CGCGGAACAA AGCAGAGAAG CAGAAGAGGG ACTTGAGTGA GGAGCTGGAG 
GCACTCAAGA CGGAGCTGGA GGACACGCTG GACACCACGG CGGCCCAGCA GGAACTGCGT 
ACAAAACGAG AACAAGAAGT GGCGGAGCTG AAGAAGGCTC TCGAGGAGGA AACGAAGAGC 
CACGAGGCTC AGATCCAGGA CATGAGGCAG AGGCATGCGA CCGCCTTGGA GGAGCTGTCG 
GAGCAGCTGG AGCAGGCCAA GAGGTTCAAA GCAAATCTGG AAAAGAACAA GCAGGGCCTG 
GAGACAGACA ACAAGGAGCT GGCGTGCGAG GTCAAGGTGC TGCAGCAGGT CAAGGCTGAG 
TCCGAGCACA AGAGGAAGAA GCTGGACGCC CAGGTCCAGG AGCTCCACGC CAAGGTCTCA 
GAGGGAGACA GACTCAGGGT GGAGCTGGCA GAGAAAGCCA ATAAGTTACA GAATGAATTG 
GATAATGTCT CAACTCTTCT AGAAGAAGCA GAGAAGAAGG GTATTAAATT TGCTAAGGAT 
GCAGCTGGTC TGGAGTCTCA GCTCCAAGAC ACACAGGAGC TTCTTCAGGA GGAGACGCGC 
CAGAAACTGA ACCTGAGCAG TCGGATCCGG CAGCTGGAGG AGGAGAGGAG CAGCCTCCAG 
GAGCAGCAGG AGGAAGAGGA GGAGGCCAGG AGGAGCCTGG AGAAGCAGCT GCAGGCCCTG 
CAGGCCCAGT TGACGGATAC CAAGAAGAAA GTAGATGATG ACCTGGGGAC AATTGAAAAT 
TTGGAAGAAG CCAAGAAGAA GCTCCTCAAG GATGTGGAGG TGCTGAGCCA GCGCCTGGAG 
GAGAAGGCCC TGGCCTATGA CAAGCTGGAG AAGACCAAGA CCCGCCTGCA GCAGGAGCTG 
GATGACCTGC TGGTAGACCT GGACCACCAG CGCCAGATCG TCTCCAACTT GGAGAAGAAG 
CAGAAGAAGT TTGATCAGCT GTTAGCGGAA GAGAAGAACA TCTCTGCCCG CTACGCAGAA 
GAGCGCGACC GGGCAGAGGC AGAGGCCCGA GAGAAGGAGA CCAAAGCCCT GTCCCTGGCC 
CGCGCCCTGG AGGAGGCCCT GGAGGCCAGG GAGGAGGCTG AGCGACAGAA TAAGCAGCTG 
CGGGCGGACA TGGAGGACCT CATGAGCTCC AAGGACGATG TGGGCAAGAA CGTTCATGAA 
CTTGAGAAAT CCAAACGGGC CCTAGAGCAG CAGGTGGAGG AAATGAGGAC CCAACTGGAG 
GAGCTGGAAG ATGAGCTCCA GGCTACAGAA GACGCCAAGC TTCGCCTAGA GGTCAACATG 
CAAGCCATGA AAGCCCAGTT TGAGAGAGAC TTGCAGACCA GGGATGAGCA GAATGAGGAA 
AAGAAGCGGC TGCTCATTAA ACAGGTGCGG GAGCTTGAGG CAGAGCTGGA GGATGAGCGG 
AAACAGCGGG CCCTGGCTGT TGCCTCGAAG AAGAAAATGG AAATAGACCT GAAGGACCTG 
GAGGCTCAAA TCGAGGCAGC TAACAAAGCA AGGGATGAAG TGATCAAGCA GCTCCGCAAA 
CTCCAGGCTC AGATGAAGGA CTACCAGCGT GAATTAGAAG AAGCTCGTGC ATCCCGGGAT 
GAGATTTTTG CTCAATCCAA AGAGAGTGAG AAGAAACTGA AAAGCCTGGA AGCAGAAATC 
CTTCAGTTGC AGGAGGAACT TGCCTCATCC GAGCGAGCCC GCCGACATGC TGAGCAGGAG 
AGAGACGAGC TGGCGGACGA GATTGCCAAT AGCGCCTCCG GCAAGTCTGC ACTCCTGGAT 
GAGAAGCGGC GTCTGGAGGC TCGGATCGCA CAGCTAGAGG AGGAGCTGGA GGAGGAGCAG 
AGCAACATGG AGCTGCTCAA TGACCGCTTC CGCAAGACCA CACTGCAGGT GGACACGCTG 
AACACTGAGC TGGCGGCCGA GCGCAGCGCT GCCCAGAAGA GCGATAATGC GCGCCAGCAG 
CTGGAGCGGC AGAACAAGGA GTTGAAGGCC AAGCTGCAGG AGCTGGAAGG CGCTGTCAAG 
TCCAAGTTCA AGGCCACCAT CTCTGCTCTA GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG 
CTTGAGCAGG AAGCCAAGGA AAGAGCCGCT GCCAATAAAC TAGTCCGTCG TACCGAGAAG 
AAGCTGAAAG AAATCTTCAT GCAGGTTGAA GATGAACGCC GCCATGCTGA CCAGTATAAA 
GAACAGATGG AGAAGGCCAA TGCCAGGATG AAGCAGCTCA AGCGGCAGCT GGAGGAAGCA 
GAGGAAGAAG CTACACGTGC CAACGCATCT CGGCGCAAAC TCCAGCGGGA ACTGGATGAT 
GCCACTGAGG CCAACGAGGG CCTGAGCCGC GAGGTCAGCA CCCTGAAGAA CCGGCTGAGG 
CGAGGCGGCC CGATCAGCTT CTCCTCCAGC CGATCAGGCC GGCGCCAGCT GCACATTGAG 
GGCGCGTCCC TGGAGCTGTC GGACGACGAC ACAGAAAGTA AGACCAGTGA CATCAACGAG 
ACACAGCCGC CGCAGTCCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_005220299.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220356.1 Bos taurus myosin, heavy chain 10, non-muscle (MYH10), transcript variant X4, mRNA.

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CloneID	OBa29702
Clone ID Related Accession (Same CDS sequence)	XM_005220299.3
Accession Version	XM_005220299.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myosin-10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220299.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTGGGGAAC TTGAACGGCA GCTTTTGCAA 
GCAAATCCAA TCCTGGAATC ATTTGGAAAC GCAAAGACTG TGAAAAATGA TAACTCATCT 
CGTTTTGGCA AGTTTATTCG GATCAACTTT GATGTAACTG GCTATATCGT TGGGGCCAAC 
ATTGAAACAT ACCTTCTGGA AAAATCTCGT GCTGTTCGTC AAGCAAAAGA TGAGCGTACT 
TTTCATATCT TTTACCAATT GTTATCTGGA GCAGGAGAAC ATCTGAAGTC TGATTTGCTC 
CTTGAAGGAT TTAATAACTA CAGATTTCTC TCCAATGGCT ATATTCCTAT TCCGGGACAA 
CAAGACAAAG ATAACTTCCA GGAGACAATG GAGGCAATGC ATATAATGGG CTTCTCCCAT 
GAAGAGATTC TGTCAATGCT TAAAGTGGTA TCTTCAGTGC TTCAGTTTGG AAATATTTCT 
TTCAAAAAGG AGCGAAACAC TGATCAAGCC TCCATGCCGG AAAATACAGT TGCACAGAAG 
CTCTGCCATC TTCTTGGCAT GAATGTGATG GAGTTCACTC GGGCCATTCT CACCCCCCGG 
ATCAAGGTCG GCCGAGACTA CGTGCAGAAA GCCCAGACCA AAGAACAAGC AGATTTTGCA 
GTAGAAGCTT TGGCAAAAGC TACCTATGAG CGGCTCTTCC GCTGGCTCGT TCACCGCATC 
AATAAAGCTC TGGACAGGAC CAAACGGCAA GGGGCCTCCT TCATTGGGAT CCTGGACATC 
GCTGGATTTG AGATTTTTGA GCTGAACTCA TTCGAGCAAC TGTGCATCAA CTACACCAAC 
GAGAAGCTGC AGCAGCTCTT CAACCACACC ATGTTCATCC TGGAGCAGGA GGAGTACCAG 
CGCGAGGGCA TCGAGTGGAA CTTCATCGAC TTTGGCCTCG ACCTGCAGCC CTGCATTGAC 
CTCATCGAGA GACCTGCTAA CCCTCCTGGC GTGCTGGCCC TTCTGGATGA AGAGTGCTGG 
TTCCCTAAAG CCACAGATAA GACCTTTGTT GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC 
TCCAAGTTTC AGAAACCACG TCAATTGAAA GACAAAGCTG ATTTTTGCAT TATCCATTAT 
GCAGGGAAGG TGGACTACAA GGCAGACGAG TGGCTGATGA AGAACATGGA CCCCCTGAAT 
GACAACGTGG CCACCCTCCT GCACCAGTCC TCTGACAGAT TCGTGGCAGA GCTCTGGAAG 
GATGAGATTC AGAATATTCA GAGAGCTTCT TTCTATGACA GTGTTTCTGG TCTTCATGAG 
CCACCAGTGG ACCGGATCGT GGGTCTGGAT CAGGTCACGG GGATGACGGA GACGGCATTC 
GGCTCCGCCT ATAAAACCAA GAAGGGCATG TTCCGCACTG TCGGGCAGCT CTACAAAGAA 
TCGCTCACCA AGCTGATGGC GACGCTCCGG AACACCAACC CGAACTTCGT GCGCTGCATC 
ATCCCAAATC ACGAGAAGCG GGCTGGGAAA CTGGACCCAC ACCTGGTGCT GGATCAGCTC 
CGCTGCAATG GCGTCCTGGA AGGGATCCGC ATCTGCCGCC AGGGCTTCCC CAACCGGATC 
GTTTTCCAGG AATTCAGACA GAGATACGAG ATCCTCACTC CAAATGCTAT CCCTAAGGGT 
TTCATGGATG GCAAACAGGC TTGTGAACGA ATGATCCGGG CTTTAGAATT GGACCCAAAC 
TTGTACAGAA TTGGACAGAG CAAGATATTT TTCAGAGCTG GAGTCTTGGC ACACTTAGAG 
GAAGAACGAG ATTTAAAAAT TACCGACATC ATTATCTTCT TCCAGGCTGT TTGCAGGGGT 
TATTTAGCCA GAAAGGCCTT CGCCAAGAAG CAGCAGCAGC TGAGTGCCCT GAAGGTGCTG 
CAGCGCAACT GTGCCGCGTA CCTGAAGCTG CGGCACTGGC AGTGGTGGCG CGTCTTCACC 
AAGGTGAAAC CACTCCTACA AGTCACTCGT CAAGAGGAGG AGCTCCAGGC CAAAGATGAA 
GAGCTGCTGA AGGTGAAAGA GAAGCAGACG AAGGTGGAAG GGGAGCTGGA GGAGATGGAG 
AGGAAGCACC AACAGCTGCT GGAGGAGAAG AATATCCTGG CAGAGCAGCT GCAGGCGGAG 
ACCGAGCTCT TTGCCGAAGC AGAGGAGATG AGGGCCAGAC TTGCCGCTAA GAAGCAGGAA 
TTAGAAGAGA TTCTCCATGA CTTGGAATCT AGGGTAGAAG AAGAAGAAGA AAGAAACCAA 
ATCCTCCAAA ATGAAAAGAA GAAAATGCAA GCACACATTC AGGACCTGGA GGAGCAGCTA 
GATGAGGAGG AGGGGGCTCG CCAGAAGCTG CAGCTGGAGA AGGTGACGGC GGAGGCCAAG 
ATCAAGAAGA TGGAAGAAGA GATTCTGCTT CTGGAGGACC AGAACTCCAA GTTTATCAAA 
GAAAAGAAAC TCATGGAAGA TCGCATTGCA GAATGTTCCT CTCAGCTGGC TGAAGAGGAA 
GAAAAGGCAA AAAACTTGGC CAAAATCAGG AATAAGCAAG AAGTAATGAT CTCGGATTTA 
GAAGAGCGCT TAAAGAAGGA GGAGAAGACG CGCCAGGAGC TGGAGAAGGC CAAAAGGAAA 
CTGGACGGGG AGACCACGGA CCTGCAAGAC CAGATCGCCG AGCTCCAGGC GCAGATTGAC 
GAGCTTAAGA TCCAGGTGGC CAAGAAAGAG GAGGAGCTGC AGGGTGCCCT GGCCAGGGGT 
GACGACGAGA CGCTACACAA GAACAACGCA CTAAAAGTCG TGCGAGAGCT GCAGGCCCAG 
ATCGCCGAGC TGCAGGAGGA CTTTGAATCT GAGAAGGCCT CGCGGAACAA AGCAGAGAAG 
CAGAAGAGGG ACTTGAGTGA GGAGCTGGAG GCACTCAAGA CGGAGCTGGA GGACACGCTG 
GACACCACGG CGGCCCAGCA GGAACTGCGT ACAAAACGAG AACAAGAAGT GGCGGAGCTG 
AAGAAGGCTC TCGAGGAGGA AACGAAGAGC CACGAGGCTC AGATCCAGGA CATGAGGCAG 
AGGCATGCGA CCGCCTTGGA GGAGCTGTCG GAGCAGCTGG AGCAGGCCAA GAGGTTCAAA 
GCAAATCTGG AAAAGAACAA GCAGGGCCTG GAGACAGACA ACAAGGAGCT GGCGTGCGAG 
GTCAAGGTGC TGCAGCAGGT CAAGGCTGAG TCCGAGCACA AGAGGAAGAA GCTGGACGCC 
CAGGTCCAGG AGCTCCACGC CAAGGTCTCA GAGGGAGACA GACTCAGGGT GGAGCTGGCA 
GAGAAAGCCA ATAAGTTACA GAATGAATTG GATAATGTCT CAACTCTTCT AGAAGAAGCA 
GAGAAGAAGG GTATTAAATT TGCTAAGGAT GCAGCTGGTC TGGAGTCTCA GCTCCAAGAC 
ACACAGGAGC TTCTTCAGGA GGAGACGCGC CAGAAACTGA ACCTGAGCAG TCGGATCCGG 
CAGCTGGAGG AGGAGAGGAG CAGCCTCCAG GAGCAGCAGG AGGAAGAGGA GGAGGCCAGG 
AGGAGCCTGG AGAAGCAGCT GCAGGCCCTG CAGGCCCAGT TGACGGATAC CAAGAAGAAA 
GTAGATGATG ACCTGGGGAC AATTGAAAAT TTGGAAGAAG CCAAGAAGAA GCTCCTCAAG 
GATGTGGAGG TGCTGAGCCA GCGCCTGGAG GAGAAGGCCC TGGCCTATGA CAAGCTGGAG 
AAGACCAAGA CCCGCCTGCA GCAGGAGCTG GATGACCTGC TGGTAGACCT GGACCACCAG 
CGCCAGATCG TCTCCAACTT GGAGAAGAAG CAGAAGAAGT TTGATCAGCT GTTAGCGGAA 
GAGAAGAACA TCTCTGCCCG CTACGCAGAA GAGCGCGACC GGGCAGAGGC AGAGGCCCGA 
GAGAAGGAGA CCAAAGCCCT GTCCCTGGCC CGCGCCCTGG AGGAGGCCCT GGAGGCCAGG 
GAGGAGGCTG AGCGACAGAA TAAGCAGCTG CGGGCGGACA TGGAGGACCT CATGAGCTCC 
AAGGACGATG TGGGCAAGAA CGTTCATGAA CTTGAGAAAT CCAAACGGGC CCTAGAGCAG 
CAGGTGGAGG AAATGAGGAC CCAACTGGAG GAGCTGGAAG ATGAGCTCCA GGCTACAGAA 
GACGCCAAGC TTCGCCTAGA GGTCAACATG CAAGCCATGA AAGCCCAGTT TGAGAGAGAC 
TTGCAGACCA GGGATGAGCA GAATGAGGAA AAGAAGCGGC TGCTCATTAA ACAGGTGCGG 
GAGCTTGAGG CAGAGCTGGA GGATGAGCGG AAACAGCGGG CCCTGGCTGT TGCCTCGAAG 
AAGAAAATGG AAATAGACCT GAAGGACCTG GAGGCTCAAA TCGAGGCAGC TAACAAAGCA 
AGGGATGAAG TGATCAAGCA GCTCCGCAAA CTCCAGGCTC AGATGAAGGA CTACCAGCGT 
GAATTAGAAG AAGCTCGTGC ATCCCGGGAT GAGATTTTTG CTCAATCCAA AGAGAGTGAG 
AAGAAACTGA AAAGCCTGGA AGCAGAAATC CTTCAGTTGC AGGAGGAACT TGCCTCATCC 
GAGCGAGCCC GCCGACATGC TGAGCAGGAG AGAGACGAGC TGGCGGACGA GATTGCCAAT 
AGCGCCTCCG GCAAGTCTGC ACTCCTGGAT GAGAAGCGGC GTCTGGAGGC TCGGATCGCA 
CAGCTAGAGG AGGAGCTGGA GGAGGAGCAG AGCAACATGG AGCTGCTCAA TGACCGCTTC 
CGCAAGACCA CACTGCAGGT GGACACGCTG AACACTGAGC TGGCGGCCGA GCGCAGCGCT 
GCCCAGAAGA GCGATAATGC GCGCCAGCAG CTGGAGCGGC AGAACAAGGA GTTGAAGGCC 
AAGCTGCAGG AGCTGGAAGG CGCTGTCAAG TCCAAGTTCA AGGCCACCAT CTCTGCTCTA 
GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG CTTGAGCAGG AAGCCAAGGA AAGAGCCGCT 
GCCAATAAAC TAGTCCGTCG TACCGAGAAG AAGCTGAAAG AAATCTTCAT GCAGGTTGAA 
GATGAACGCC GCCATGCTGA CCAGTATAAA GAACAGATGG AGAAGGCCAA TGCCAGGATG 
AAGCAGCTCA AGCGGCAGCT GGAGGAAGCA GAGGAAGAAG CTACACGTGC CAACGCATCT 
CGGCGCAAAC TCCAGCGGGA ACTGGATGAT GCCACTGAGG CCAACGAGGG CCTGAGCCGC 
GAGGTCAGCA CCCTGAAGAA CCGGCTGAGG CGAGGCGGCC CGATCAGCTT CTCCTCCAGC 
CGATCAGGCC GGCGCCAGCT GCACATTGAG GGCGCGTCCC TGGAGCTGTC GGACGACGAC 
ACAGAAAGTA AGACCAGTGA CATCAACGAG ACACAGCCGC CGCAGTCCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220356.1
CDS	178..6171
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVLVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDEIQNIQRASFYDSVSGLHEPPVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKEKQTKVEGELEEMERKHQQLLEEKNILAEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKIQVAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETKSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSTLLEEAEKKGIKFAKDAAGLESQLQDTQELLQEETRQKLNLSSRIRQLEEERSSLQEQQEEEEEARRSLEKQLQALQAQLTDTKKKVDDDLGTIENLEEAKKKLLKDVEVLSQRLEEKALAYDKLEKTKTRLQQELDDLLVDLDHQRQIVSNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALEAREEAERQNKQLRADMEDLMSSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLIKQVRELEAELEDERKQRALAVASKKKMEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNTELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDINETQPPQSE

Target ORF information:

RefSeq Version	XM_005220299.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin, heavy chain 10, non-muscle (MYH10), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220299.3

ORF Insert Sequence:

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTGGGGAAC TTGAACGGCA GCTTTTGCAA 
GCAAATCCAA TCCTGGAATC ATTTGGAAAC GCAAAGACTG TGAAAAATGA TAACTCATCT 
CGTTTTGGCA AGTTTATTCG GATCAACTTT GATGTAACTG GCTATATCGT TGGGGCCAAC 
ATTGAAACAT ACCTTCTGGA AAAATCTCGT GCTGTTCGTC AAGCAAAAGA TGAGCGTACT 
TTTCATATCT TTTACCAATT GTTATCTGGA GCAGGAGAAC ATCTGAAGTC TGATTTGCTC 
CTTGAAGGAT TTAATAACTA CAGATTTCTC TCCAATGGCT ATATTCCTAT TCCGGGACAA 
CAAGACAAAG ATAACTTCCA GGAGACAATG GAGGCAATGC ATATAATGGG CTTCTCCCAT 
GAAGAGATTC TGTCAATGCT TAAAGTGGTA TCTTCAGTGC TTCAGTTTGG AAATATTTCT 
TTCAAAAAGG AGCGAAACAC TGATCAAGCC TCCATGCCGG AAAATACAGT TGCACAGAAG 
CTCTGCCATC TTCTTGGCAT GAATGTGATG GAGTTCACTC GGGCCATTCT CACCCCCCGG 
ATCAAGGTCG GCCGAGACTA CGTGCAGAAA GCCCAGACCA AAGAACAAGC AGATTTTGCA 
GTAGAAGCTT TGGCAAAAGC TACCTATGAG CGGCTCTTCC GCTGGCTCGT TCACCGCATC 
AATAAAGCTC TGGACAGGAC CAAACGGCAA GGGGCCTCCT TCATTGGGAT CCTGGACATC 
GCTGGATTTG AGATTTTTGA GCTGAACTCA TTCGAGCAAC TGTGCATCAA CTACACCAAC 
GAGAAGCTGC AGCAGCTCTT CAACCACACC ATGTTCATCC TGGAGCAGGA GGAGTACCAG 
CGCGAGGGCA TCGAGTGGAA CTTCATCGAC TTTGGCCTCG ACCTGCAGCC CTGCATTGAC 
CTCATCGAGA GACCTGCTAA CCCTCCTGGC GTGCTGGCCC TTCTGGATGA AGAGTGCTGG 
TTCCCTAAAG CCACAGATAA GACCTTTGTT GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC 
TCCAAGTTTC AGAAACCACG TCAATTGAAA GACAAAGCTG ATTTTTGCAT TATCCATTAT 
GCAGGGAAGG TGGACTACAA GGCAGACGAG TGGCTGATGA AGAACATGGA CCCCCTGAAT 
GACAACGTGG CCACCCTCCT GCACCAGTCC TCTGACAGAT TCGTGGCAGA GCTCTGGAAG 
GATGAGATTC AGAATATTCA GAGAGCTTCT TTCTATGACA GTGTTTCTGG TCTTCATGAG 
CCACCAGTGG ACCGGATCGT GGGTCTGGAT CAGGTCACGG GGATGACGGA GACGGCATTC 
GGCTCCGCCT ATAAAACCAA GAAGGGCATG TTCCGCACTG TCGGGCAGCT CTACAAAGAA 
TCGCTCACCA AGCTGATGGC GACGCTCCGG AACACCAACC CGAACTTCGT GCGCTGCATC 
ATCCCAAATC ACGAGAAGCG GGCTGGGAAA CTGGACCCAC ACCTGGTGCT GGATCAGCTC 
CGCTGCAATG GCGTCCTGGA AGGGATCCGC ATCTGCCGCC AGGGCTTCCC CAACCGGATC 
GTTTTCCAGG AATTCAGACA GAGATACGAG ATCCTCACTC CAAATGCTAT CCCTAAGGGT 
TTCATGGATG GCAAACAGGC TTGTGAACGA ATGATCCGGG CTTTAGAATT GGACCCAAAC 
TTGTACAGAA TTGGACAGAG CAAGATATTT TTCAGAGCTG GAGTCTTGGC ACACTTAGAG 
GAAGAACGAG ATTTAAAAAT TACCGACATC ATTATCTTCT TCCAGGCTGT TTGCAGGGGT 
TATTTAGCCA GAAAGGCCTT CGCCAAGAAG CAGCAGCAGC TGAGTGCCCT GAAGGTGCTG 
CAGCGCAACT GTGCCGCGTA CCTGAAGCTG CGGCACTGGC AGTGGTGGCG CGTCTTCACC 
AAGGTGAAAC CACTCCTACA AGTCACTCGT CAAGAGGAGG AGCTCCAGGC CAAAGATGAA 
GAGCTGCTGA AGGTGAAAGA GAAGCAGACG AAGGTGGAAG GGGAGCTGGA GGAGATGGAG 
AGGAAGCACC AACAGCTGCT GGAGGAGAAG AATATCCTGG CAGAGCAGCT GCAGGCGGAG 
ACCGAGCTCT TTGCCGAAGC AGAGGAGATG AGGGCCAGAC TTGCCGCTAA GAAGCAGGAA 
TTAGAAGAGA TTCTCCATGA CTTGGAATCT AGGGTAGAAG AAGAAGAAGA AAGAAACCAA 
ATCCTCCAAA ATGAAAAGAA GAAAATGCAA GCACACATTC AGGACCTGGA GGAGCAGCTA 
GATGAGGAGG AGGGGGCTCG CCAGAAGCTG CAGCTGGAGA AGGTGACGGC GGAGGCCAAG 
ATCAAGAAGA TGGAAGAAGA GATTCTGCTT CTGGAGGACC AGAACTCCAA GTTTATCAAA 
GAAAAGAAAC TCATGGAAGA TCGCATTGCA GAATGTTCCT CTCAGCTGGC TGAAGAGGAA 
GAAAAGGCAA AAAACTTGGC CAAAATCAGG AATAAGCAAG AAGTAATGAT CTCGGATTTA 
GAAGAGCGCT TAAAGAAGGA GGAGAAGACG CGCCAGGAGC TGGAGAAGGC CAAAAGGAAA 
CTGGACGGGG AGACCACGGA CCTGCAAGAC CAGATCGCCG AGCTCCAGGC GCAGATTGAC 
GAGCTTAAGA TCCAGGTGGC CAAGAAAGAG GAGGAGCTGC AGGGTGCCCT GGCCAGGGGT 
GACGACGAGA CGCTACACAA GAACAACGCA CTAAAAGTCG TGCGAGAGCT GCAGGCCCAG 
ATCGCCGAGC TGCAGGAGGA CTTTGAATCT GAGAAGGCCT CGCGGAACAA AGCAGAGAAG 
CAGAAGAGGG ACTTGAGTGA GGAGCTGGAG GCACTCAAGA CGGAGCTGGA GGACACGCTG 
GACACCACGG CGGCCCAGCA GGAACTGCGT ACAAAACGAG AACAAGAAGT GGCGGAGCTG 
AAGAAGGCTC TCGAGGAGGA AACGAAGAGC CACGAGGCTC AGATCCAGGA CATGAGGCAG 
AGGCATGCGA CCGCCTTGGA GGAGCTGTCG GAGCAGCTGG AGCAGGCCAA GAGGTTCAAA 
GCAAATCTGG AAAAGAACAA GCAGGGCCTG GAGACAGACA ACAAGGAGCT GGCGTGCGAG 
GTCAAGGTGC TGCAGCAGGT CAAGGCTGAG TCCGAGCACA AGAGGAAGAA GCTGGACGCC 
CAGGTCCAGG AGCTCCACGC CAAGGTCTCA GAGGGAGACA GACTCAGGGT GGAGCTGGCA 
GAGAAAGCCA ATAAGTTACA GAATGAATTG GATAATGTCT CAACTCTTCT AGAAGAAGCA 
GAGAAGAAGG GTATTAAATT TGCTAAGGAT GCAGCTGGTC TGGAGTCTCA GCTCCAAGAC 
ACACAGGAGC TTCTTCAGGA GGAGACGCGC CAGAAACTGA ACCTGAGCAG TCGGATCCGG 
CAGCTGGAGG AGGAGAGGAG CAGCCTCCAG GAGCAGCAGG AGGAAGAGGA GGAGGCCAGG 
AGGAGCCTGG AGAAGCAGCT GCAGGCCCTG CAGGCCCAGT TGACGGATAC CAAGAAGAAA 
GTAGATGATG ACCTGGGGAC AATTGAAAAT TTGGAAGAAG CCAAGAAGAA GCTCCTCAAG 
GATGTGGAGG TGCTGAGCCA GCGCCTGGAG GAGAAGGCCC TGGCCTATGA CAAGCTGGAG 
AAGACCAAGA CCCGCCTGCA GCAGGAGCTG GATGACCTGC TGGTAGACCT GGACCACCAG 
CGCCAGATCG TCTCCAACTT GGAGAAGAAG CAGAAGAAGT TTGATCAGCT GTTAGCGGAA 
GAGAAGAACA TCTCTGCCCG CTACGCAGAA GAGCGCGACC GGGCAGAGGC AGAGGCCCGA 
GAGAAGGAGA CCAAAGCCCT GTCCCTGGCC CGCGCCCTGG AGGAGGCCCT GGAGGCCAGG 
GAGGAGGCTG AGCGACAGAA TAAGCAGCTG CGGGCGGACA TGGAGGACCT CATGAGCTCC 
AAGGACGATG TGGGCAAGAA CGTTCATGAA CTTGAGAAAT CCAAACGGGC CCTAGAGCAG 
CAGGTGGAGG AAATGAGGAC CCAACTGGAG GAGCTGGAAG ATGAGCTCCA GGCTACAGAA 
GACGCCAAGC TTCGCCTAGA GGTCAACATG CAAGCCATGA AAGCCCAGTT TGAGAGAGAC 
TTGCAGACCA GGGATGAGCA GAATGAGGAA AAGAAGCGGC TGCTCATTAA ACAGGTGCGG 
GAGCTTGAGG CAGAGCTGGA GGATGAGCGG AAACAGCGGG CCCTGGCTGT TGCCTCGAAG 
AAGAAAATGG AAATAGACCT GAAGGACCTG GAGGCTCAAA TCGAGGCAGC TAACAAAGCA 
AGGGATGAAG TGATCAAGCA GCTCCGCAAA CTCCAGGCTC AGATGAAGGA CTACCAGCGT 
GAATTAGAAG AAGCTCGTGC ATCCCGGGAT GAGATTTTTG CTCAATCCAA AGAGAGTGAG 
AAGAAACTGA AAAGCCTGGA AGCAGAAATC CTTCAGTTGC AGGAGGAACT TGCCTCATCC 
GAGCGAGCCC GCCGACATGC TGAGCAGGAG AGAGACGAGC TGGCGGACGA GATTGCCAAT 
AGCGCCTCCG GCAAGTCTGC ACTCCTGGAT GAGAAGCGGC GTCTGGAGGC TCGGATCGCA 
CAGCTAGAGG AGGAGCTGGA GGAGGAGCAG AGCAACATGG AGCTGCTCAA TGACCGCTTC 
CGCAAGACCA CACTGCAGGT GGACACGCTG AACACTGAGC TGGCGGCCGA GCGCAGCGCT 
GCCCAGAAGA GCGATAATGC GCGCCAGCAG CTGGAGCGGC AGAACAAGGA GTTGAAGGCC 
AAGCTGCAGG AGCTGGAAGG CGCTGTCAAG TCCAAGTTCA AGGCCACCAT CTCTGCTCTA 
GAAGCCAAGA TTGGGCAGCT GGAGGAGCAG CTTGAGCAGG AAGCCAAGGA AAGAGCCGCT 
GCCAATAAAC TAGTCCGTCG TACCGAGAAG AAGCTGAAAG AAATCTTCAT GCAGGTTGAA 
GATGAACGCC GCCATGCTGA CCAGTATAAA GAACAGATGG AGAAGGCCAA TGCCAGGATG 
AAGCAGCTCA AGCGGCAGCT GGAGGAAGCA GAGGAAGAAG CTACACGTGC CAACGCATCT 
CGGCGCAAAC TCCAGCGGGA ACTGGATGAT GCCACTGAGG CCAACGAGGG CCTGAGCCGC 
GAGGTCAGCA CCCTGAAGAA CCGGCTGAGG CGAGGCGGCC CGATCAGCTT CTCCTCCAGC 
CGATCAGGCC GGCGCCAGCT GCACATTGAG GGCGCGTCCC TGGAGCTGTC GGACGACGAC 
ACAGAAAGTA AGACCAGTGA CATCAACGAG ACACAGCCGC CGCAGTCCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_015458671.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314157.1 Bos taurus myosin, heavy chain 10, non-muscle (MYH10), transcript variant X5, mRNA.

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CloneID	OBa29703
Clone ID Related Accession (Same CDS sequence)	XM_015458671.1
Accession Version	XM_015458671.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myosin-10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTCAGGAAT CACCTAAACC AGTGAAACAC 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC ATTTGGAAAC 
GCAAAGACTG TGAAAAATGA TAACTCATCT CGTTTTGGCA AGTTTATTCG GATCAACTTT 
GATGTAACTG GCTATATCGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAATT GTTATCTGGA 
GCAGGAGAAC ATCTGAAGTC TGATTTGCTC CTTGAAGGAT TTAATAACTA CAGATTTCTC 
TCCAATGGCT ATATTCCTAT TCCGGGACAA CAAGACAAAG ATAACTTCCA GGAGACAATG 
GAGGCAATGC ATATAATGGG CTTCTCCCAT GAAGAGATTC TGTCAATGCT TAAAGTGGTA 
TCTTCAGTGC TTCAGTTTGG AAATATTTCT TTCAAAAAGG AGCGAAACAC TGATCAAGCC 
TCCATGCCGG AAAATACAGT TGCACAGAAG CTCTGCCATC TTCTTGGCAT GAATGTGATG 
GAGTTCACTC GGGCCATTCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA CGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCA GTAGAAGCTT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GCTGGCTCGT TCACCGCATC AATAAAGCTC TGGACAGGAC CAAACGGCAA 
GGGGCCTCCT TCATTGGGAT CCTGGACATC GCTGGATTTG AGATTTTTGA GCTGAACTCA 
TTCGAGCAAC TGTGCATCAA CTACACCAAC GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAA CTTCATCGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATTGAC CTCATCGAGA GACCTGCTAA CCCTCCTGGC 
GTGCTGGCCC TTCTGGATGA AGAGTGCTGG TTCCCTAAAG CCACAGATAA GACCTTTGTT 
GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC TCCAAGTTTC AGAAACCACG TCAATTGAAA 
GACAAAGCTG ATTTTTGCAT TATCCATTAT GCAGGGAAGG TGGACTACAA GGCAGACGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAT GACAACGTGG CCACCCTCCT GCACCAGTCC 
TCTGACAGAT TCGTGGCAGA GCTCTGGAAG GATGTGGACC GGATCGTGGG TCTGGATCAG 
GTCACGGGGA TGACGGAGAC GGCATTCGGC TCCGCCTATA AAACCAAGAA GGGCATGTTC 
CGCACTGTCG GGCAGCTCTA CAAAGAATCG CTCACCAAGC TGATGGCGAC GCTCCGGAAC 
ACCAACCCGA ACTTCGTGCG CTGCATCATC CCAAATCACG AGAAGCGGGC TGGGAAACTG 
GACCCACACC TGGTGCTGGA TCAGCTCCGC TGCAATGGCG TCCTGGAAGG GATCCGCATC 
TGCCGCCAGG GCTTCCCCAA CCGGATCGTT TTCCAGGAAT TCAGACAGAG ATACGAGATC 
CTCACTCCAA ATGCTATCCC TAAGGGTTTC ATGGATGGCA AACAGGCTTG TGAACGAATG 
ATCCGGGCTT TAGAATTGGA CCCAAACTTG TACAGAATTG GACAGAGCAA GATATTTTTC 
AGAGCTGGAG TCTTGGCACA CTTAGAGGAA GAACGAGATT TAAAAATTAC CGACATCATT 
ATCTTCTTCC AGGCTGTTTG CAGGGGTTAT TTAGCCAGAA AGGCCTTCGC CAAGAAGCAG 
CAGCAGCTGA GTGCCCTGAA GGTGCTGCAG CGCAACTGTG CCGCGTACCT GAAGCTGCGG 
CACTGGCAGT GGTGGCGCGT CTTCACCAAG GTGAAACCAC TCCTACAAGT CACTCGTCAA 
GAGGAGGAGC TCCAGGCCAA AGATGAAGAG CTGCTGAAGG TGAAAGAGAA GCAGACGAAG 
GTGGAAGGGG AGCTGGAGGA GATGGAGAGG AAGCACCAAC AGCTGCTGGA GGAGAAGAAT 
ATCCTGGCAG AGCAGCTGCA GGCGGAGACC GAGCTCTTTG CCGAAGCAGA GGAGATGAGG 
GCCAGACTTG CCGCTAAGAA GCAGGAATTA GAAGAGATTC TCCATGACTT GGAATCTAGG 
GTAGAAGAAG AAGAAGAAAG AAACCAAATC CTCCAAAATG AAAAGAAGAA AATGCAAGCA 
CACATTCAGG ACCTGGAGGA GCAGCTAGAT GAGGAGGAGG GGGCTCGCCA GAAGCTGCAG 
CTGGAGAAGG TGACGGCGGA GGCCAAGATC AAGAAGATGG AAGAAGAGAT TCTGCTTCTG 
GAGGACCAGA ACTCCAAGTT TATCAAAGAA AAGAAACTCA TGGAAGATCG CATTGCAGAA 
TGTTCCTCTC AGCTGGCTGA AGAGGAAGAA AAGGCAAAAA ACTTGGCCAA AATCAGGAAT 
AAGCAAGAAG TAATGATCTC GGATTTAGAA GAGCGCTTAA AGAAGGAGGA GAAGACGCGC 
CAGGAGCTGG AGAAGGCCAA AAGGAAACTG GACGGGGAGA CCACGGACCT GCAAGACCAG 
ATCGCCGAGC TCCAGGCGCA GATTGACGAG CTTAAGATCC AGGTGGCCAA GAAAGAGGAG 
GAGCTGCAGG GTGCCCTGGC CAGGGGTGAC GACGAGACGC TACACAAGAA CAACGCACTA 
AAAGTCGTGC GAGAGCTGCA GGCCCAGATC GCCGAGCTGC AGGAGGACTT TGAATCTGAG 
AAGGCCTCGC GGAACAAAGC AGAGAAGCAG AAGAGGGACT TGAGTGAGGA GCTGGAGGCA 
CTCAAGACGG AGCTGGAGGA CACGCTGGAC ACCACGGCGG CCCAGCAGGA ACTGCGTACA 
AAACGAGAAC AAGAAGTGGC GGAGCTGAAG AAGGCTCTCG AGGAGGAAAC GAAGAGCCAC 
GAGGCTCAGA TCCAGGACAT GAGGCAGAGG CATGCGACCG CCTTGGAGGA GCTGTCGGAG 
CAGCTGGAGC AGGCCAAGAG GTTCAAAGCA AATCTGGAAA AGAACAAGCA GGGCCTGGAG 
ACAGACAACA AGGAGCTGGC GTGCGAGGTC AAGGTGCTGC AGCAGGTCAA GGCTGAGTCC 
GAGCACAAGA GGAAGAAGCT GGACGCCCAG GTCCAGGAGC TCCACGCCAA GGTCTCAGAG 
GGAGACAGAC TCAGGGTGGA GCTGGCAGAG AAAGCCAATA AGTTACAGAA TGAATTGGAT 
AATGTCTCAA CTCTTCTAGA AGAAGCAGAG AAGAAGGGTA TTAAATTTGC TAAGGATGCA 
GCTGGTCTGG AGTCTCAGCT CCAAGACACA CAGGAGCTTC TTCAGGAGGA GACGCGCCAG 
AAACTGAACC TGAGCAGTCG GATCCGGCAG CTGGAGGAGG AGAGGAGCAG CCTCCAGGAG 
CAGCAGGAGG AAGAGGAGGA GGCCAGGAGG AGCCTGGAGA AGCAGCTGCA GGCCCTGCAG 
GCCCAGTTGA CGGATACCAA GAAGAAAGTA GATGATGACC TGGGGACAAT TGAAAATTTG 
GAAGAAGCCA AGAAGAAGCT CCTCAAGGAT GTGGAGGTGC TGAGCCAGCG CCTGGAGGAG 
AAGGCCCTGG CCTATGACAA GCTGGAGAAG ACCAAGACCC GCCTGCAGCA GGAGCTGGAT 
GACCTGCTGG TAGACCTGGA CCACCAGCGC CAGATCGTCT CCAACTTGGA GAAGAAGCAG 
AAGAAGTTTG ATCAGCTGTT AGCGGAAGAG AAGAACATCT CTGCCCGCTA CGCAGAAGAG 
CGCGACCGGG CAGAGGCAGA GGCCCGAGAG AAGGAGACCA AAGCCCTGTC CCTGGCCCGC 
GCCCTGGAGG AGGCCCTGGA GGCCAGGGAG GAGGCTGAGC GACAGAATAA GCAGCTGCGG 
GCGGACATGG AGGACCTCAT GAGCTCCAAG GACGATGTGG GCAAGAACGT TCATGAACTT 
GAGAAATCCA AACGGGCCCT AGAGCAGCAG GTGGAGGAAA TGAGGACCCA ACTGGAGGAG 
CTGGAAGATG AGCTCCAGGC TACAGAAGAC GCCAAGCTTC GCCTAGAGGT CAACATGCAA 
GCCATGAAAG CCCAGTTTGA GAGAGACTTG CAGACCAGGG ATGAGCAGAA TGAGGAAAAG 
AAGCGGCTGC TCATTAAACA GGTGCGGGAG CTTGAGGCAG AGCTGGAGGA TGAGCGGAAA 
CAGCGGGCCC TGGCTGTTGC CTCGAAGAAG AAAATGGAAA TAGACCTGAA GGACCTGGAG 
GCTCAAATCG AGGCAGCTAA CAAAGCAAGG GATGAAGTGA TCAAGCAGCT CCGCAAACTC 
CAGGCTCAGA TGAAGGACTA CCAGCGTGAA TTAGAAGAAG CTCGTGCATC CCGGGATGAG 
ATTTTTGCTC AATCCAAAGA GAGTGAGAAG AAACTGAAAA GCCTGGAAGC AGAAATCCTT 
CAGTTGCAGG AGGAACTTGC CTCATCCGAG CGAGCCCGCC GACATGCTGA GCAGGAGAGA 
GACGAGCTGG CGGACGAGAT TGCCAATAGC GCCTCCGGCA AGTCTGCACT CCTGGATGAG 
AAGCGGCGTC TGGAGGCTCG GATCGCACAG CTAGAGGAGG AGCTGGAGGA GGAGCAGAGC 
AACATGGAGC TGCTCAATGA CCGCTTCCGC AAGACCACAC TGCAGGTGGA CACGCTGAAC 
ACTGAGCTGG CGGCCGAGCG CAGCGCTGCC CAGAAGAGCG ATAATGCGCG CCAGCAGCTG 
GAGCGGCAGA ACAAGGAGTT GAAGGCCAAG CTGCAGGAGC TGGAAGGCGC TGTCAAGTCC 
AAGTTCAAGG CCACCATCTC TGCTCTAGAA GCCAAGATTG GGCAGCTGGA GGAGCAGCTT 
GAGCAGGAAG CCAAGGAAAG AGCCGCTGCC AATAAACTAG TCCGTCGTAC CGAGAAGAAG 
CTGAAAGAAA TCTTCATGCA GGTTGAAGAT GAACGCCGCC ATGCTGACCA GTATAAAGAA 
CAGATGGAGA AGGCCAATGC CAGGATGAAG CAGCTCAAGC GGCAGCTGGA GGAAGCAGAG 
GAAGAAGCTA CACGTGCCAA CGCATCTCGG CGCAAACTCC AGCGGGAACT GGATGATGCC 
ACTGAGGCCA ACGAGGGCCT GAGCCGCGAG GTCAGCACCC TGAAGAACCG GCTGAGGCGA 
GGCGGCCCGA TCAGCTTCTC CTCCAGCCGA TCAGGCCGGC GCCAGCTGCA CATTGAGGGC 
GCGTCCCTGG AGCTGTCGGA CGACGACACA GAAAGTAAGA CCAGTGACAT CAACGAGACA 
CAGCCGCCGC AGTCCGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314157.1
CDS	1..5961
Translation	MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVLVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPQESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKEKQTKVEGELEEMERKHQQLLEEKNILAEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKIQVAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETKSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSTLLEEAEKKGIKFAKDAAGLESQLQDTQELLQEETRQKLNLSSRIRQLEEERSSLQEQQEEEEEARRSLEKQLQALQAQLTDTKKKVDDDLGTIENLEEAKKKLLKDVEVLSQRLEEKALAYDKLEKTKTRLQQELDDLLVDLDHQRQIVSNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALEAREEAERQNKQLRADMEDLMSSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLIKQVRELEAELEDERKQRALAVASKKKMEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNTELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDINETQPPQSE

Target ORF information:

RefSeq Version	XM_015458671.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin, heavy chain 10, non-muscle (MYH10), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458671.1

ORF Insert Sequence:

ATGGCACAGA GAACTGGACT CGAAGATCCA GAGAGGTATC TCTTTGTGGA CAGGGCAGTC 
ATCTACAACC CTGCCACTCA GGCTGATTGG ACGGCCAAAA AGCTAGTGTG GATCCCGTCA 
GAACGCCATG GTTTTGAGGC AGCTAGTATC AAAGAAGAAC GGGGAGATGA AGTTCTGGTG 
GAGTTGGCAG AGAATGGAAA GAAAGCAATG GTCAACAAAG ATGATATTCA GAAGATGAAC 
CCACCTAAGT TTTCCAAGGT GGAGGATATG GCAGAATTGA CATGCTTGAA TGAAGCTTCT 
GTTCTACATA ATCTGAAGGA TCGTTACTAT TCAGGACTTA TCTATACCTA TTCTGGACTC 
TTCTGTGTAG TCATAAATCC TTACAAGAAC CTTCCAATTT ACTCTGAGAA TATTATTGAA 
ATGTACAGAG GGAAGAAGCG TCACGAGATG CCTCCACACA TCTACGCTAT ATCAGAATCT 
GCTTACAGAT GCATGCTTCA AGATCGTGAG GACCAGTCAA TTCTTTGCAC GGGCGAATCA 
GGTGCCGGGA AGACAGAAAA CACTAAGAAA GTCATTCAGT ACCTTGCTCA TGTTGCTTCT 
TCACACAAAG GACGAAAGGA CCATAATATT CCTCAGGAAT CACCTAAACC AGTGAAACAC 
CAGGGGGAAC TTGAACGGCA GCTTTTGCAA GCAAATCCAA TCCTGGAATC ATTTGGAAAC 
GCAAAGACTG TGAAAAATGA TAACTCATCT CGTTTTGGCA AGTTTATTCG GATCAACTTT 
GATGTAACTG GCTATATCGT TGGGGCCAAC ATTGAAACAT ACCTTCTGGA AAAATCTCGT 
GCTGTTCGTC AAGCAAAAGA TGAGCGTACT TTTCATATCT TTTACCAATT GTTATCTGGA 
GCAGGAGAAC ATCTGAAGTC TGATTTGCTC CTTGAAGGAT TTAATAACTA CAGATTTCTC 
TCCAATGGCT ATATTCCTAT TCCGGGACAA CAAGACAAAG ATAACTTCCA GGAGACAATG 
GAGGCAATGC ATATAATGGG CTTCTCCCAT GAAGAGATTC TGTCAATGCT TAAAGTGGTA 
TCTTCAGTGC TTCAGTTTGG AAATATTTCT TTCAAAAAGG AGCGAAACAC TGATCAAGCC 
TCCATGCCGG AAAATACAGT TGCACAGAAG CTCTGCCATC TTCTTGGCAT GAATGTGATG 
GAGTTCACTC GGGCCATTCT CACCCCCCGG ATCAAGGTCG GCCGAGACTA CGTGCAGAAA 
GCCCAGACCA AAGAACAAGC AGATTTTGCA GTAGAAGCTT TGGCAAAAGC TACCTATGAG 
CGGCTCTTCC GCTGGCTCGT TCACCGCATC AATAAAGCTC TGGACAGGAC CAAACGGCAA 
GGGGCCTCCT TCATTGGGAT CCTGGACATC GCTGGATTTG AGATTTTTGA GCTGAACTCA 
TTCGAGCAAC TGTGCATCAA CTACACCAAC GAGAAGCTGC AGCAGCTCTT CAACCACACC 
ATGTTCATCC TGGAGCAGGA GGAGTACCAG CGCGAGGGCA TCGAGTGGAA CTTCATCGAC 
TTTGGCCTCG ACCTGCAGCC CTGCATTGAC CTCATCGAGA GACCTGCTAA CCCTCCTGGC 
GTGCTGGCCC TTCTGGATGA AGAGTGCTGG TTCCCTAAAG CCACAGATAA GACCTTTGTT 
GAAAAGCTCG TTCAAGAGCA AGGGTCCCAC TCCAAGTTTC AGAAACCACG TCAATTGAAA 
GACAAAGCTG ATTTTTGCAT TATCCATTAT GCAGGGAAGG TGGACTACAA GGCAGACGAG 
TGGCTGATGA AGAACATGGA CCCCCTGAAT GACAACGTGG CCACCCTCCT GCACCAGTCC 
TCTGACAGAT TCGTGGCAGA GCTCTGGAAG GATGTGGACC GGATCGTGGG TCTGGATCAG 
GTCACGGGGA TGACGGAGAC GGCATTCGGC TCCGCCTATA AAACCAAGAA GGGCATGTTC 
CGCACTGTCG GGCAGCTCTA CAAAGAATCG CTCACCAAGC TGATGGCGAC GCTCCGGAAC 
ACCAACCCGA ACTTCGTGCG CTGCATCATC CCAAATCACG AGAAGCGGGC TGGGAAACTG 
GACCCACACC TGGTGCTGGA TCAGCTCCGC TGCAATGGCG TCCTGGAAGG GATCCGCATC 
TGCCGCCAGG GCTTCCCCAA CCGGATCGTT TTCCAGGAAT TCAGACAGAG ATACGAGATC 
CTCACTCCAA ATGCTATCCC TAAGGGTTTC ATGGATGGCA AACAGGCTTG TGAACGAATG 
ATCCGGGCTT TAGAATTGGA CCCAAACTTG TACAGAATTG GACAGAGCAA GATATTTTTC 
AGAGCTGGAG TCTTGGCACA CTTAGAGGAA GAACGAGATT TAAAAATTAC CGACATCATT 
ATCTTCTTCC AGGCTGTTTG CAGGGGTTAT TTAGCCAGAA AGGCCTTCGC CAAGAAGCAG 
CAGCAGCTGA GTGCCCTGAA GGTGCTGCAG CGCAACTGTG CCGCGTACCT GAAGCTGCGG 
CACTGGCAGT GGTGGCGCGT CTTCACCAAG GTGAAACCAC TCCTACAAGT CACTCGTCAA 
GAGGAGGAGC TCCAGGCCAA AGATGAAGAG CTGCTGAAGG TGAAAGAGAA GCAGACGAAG 
GTGGAAGGGG AGCTGGAGGA GATGGAGAGG AAGCACCAAC AGCTGCTGGA GGAGAAGAAT 
ATCCTGGCAG AGCAGCTGCA GGCGGAGACC GAGCTCTTTG CCGAAGCAGA GGAGATGAGG 
GCCAGACTTG CCGCTAAGAA GCAGGAATTA GAAGAGATTC TCCATGACTT GGAATCTAGG 
GTAGAAGAAG AAGAAGAAAG AAACCAAATC CTCCAAAATG AAAAGAAGAA AATGCAAGCA 
CACATTCAGG ACCTGGAGGA GCAGCTAGAT GAGGAGGAGG GGGCTCGCCA GAAGCTGCAG 
CTGGAGAAGG TGACGGCGGA GGCCAAGATC AAGAAGATGG AAGAAGAGAT TCTGCTTCTG 
GAGGACCAGA ACTCCAAGTT TATCAAAGAA AAGAAACTCA TGGAAGATCG CATTGCAGAA 
TGTTCCTCTC AGCTGGCTGA AGAGGAAGAA AAGGCAAAAA ACTTGGCCAA AATCAGGAAT 
AAGCAAGAAG TAATGATCTC GGATTTAGAA GAGCGCTTAA AGAAGGAGGA GAAGACGCGC 
CAGGAGCTGG AGAAGGCCAA AAGGAAACTG GACGGGGAGA CCACGGACCT GCAAGACCAG 
ATCGCCGAGC TCCAGGCGCA GATTGACGAG CTTAAGATCC AGGTGGCCAA GAAAGAGGAG 
GAGCTGCAGG GTGCCCTGGC CAGGGGTGAC GACGAGACGC TACACAAGAA CAACGCACTA 
AAAGTCGTGC GAGAGCTGCA GGCCCAGATC GCCGAGCTGC AGGAGGACTT TGAATCTGAG 
AAGGCCTCGC GGAACAAAGC AGAGAAGCAG AAGAGGGACT TGAGTGAGGA GCTGGAGGCA 
CTCAAGACGG AGCTGGAGGA CACGCTGGAC ACCACGGCGG CCCAGCAGGA ACTGCGTACA 
AAACGAGAAC AAGAAGTGGC GGAGCTGAAG AAGGCTCTCG AGGAGGAAAC GAAGAGCCAC 
GAGGCTCAGA TCCAGGACAT GAGGCAGAGG CATGCGACCG CCTTGGAGGA GCTGTCGGAG 
CAGCTGGAGC AGGCCAAGAG GTTCAAAGCA AATCTGGAAA AGAACAAGCA GGGCCTGGAG 
ACAGACAACA AGGAGCTGGC GTGCGAGGTC AAGGTGCTGC AGCAGGTCAA GGCTGAGTCC 
GAGCACAAGA GGAAGAAGCT GGACGCCCAG GTCCAGGAGC TCCACGCCAA GGTCTCAGAG 
GGAGACAGAC TCAGGGTGGA GCTGGCAGAG AAAGCCAATA AGTTACAGAA TGAATTGGAT 
AATGTCTCAA CTCTTCTAGA AGAAGCAGAG AAGAAGGGTA TTAAATTTGC TAAGGATGCA 
GCTGGTCTGG AGTCTCAGCT CCAAGACACA CAGGAGCTTC TTCAGGAGGA GACGCGCCAG 
AAACTGAACC TGAGCAGTCG GATCCGGCAG CTGGAGGAGG AGAGGAGCAG CCTCCAGGAG 
CAGCAGGAGG AAGAGGAGGA GGCCAGGAGG AGCCTGGAGA AGCAGCTGCA GGCCCTGCAG 
GCCCAGTTGA CGGATACCAA GAAGAAAGTA GATGATGACC TGGGGACAAT TGAAAATTTG 
GAAGAAGCCA AGAAGAAGCT CCTCAAGGAT GTGGAGGTGC TGAGCCAGCG CCTGGAGGAG 
AAGGCCCTGG CCTATGACAA GCTGGAGAAG ACCAAGACCC GCCTGCAGCA GGAGCTGGAT 
GACCTGCTGG TAGACCTGGA CCACCAGCGC CAGATCGTCT CCAACTTGGA GAAGAAGCAG 
AAGAAGTTTG ATCAGCTGTT AGCGGAAGAG AAGAACATCT CTGCCCGCTA CGCAGAAGAG 
CGCGACCGGG CAGAGGCAGA GGCCCGAGAG AAGGAGACCA AAGCCCTGTC CCTGGCCCGC 
GCCCTGGAGG AGGCCCTGGA GGCCAGGGAG GAGGCTGAGC GACAGAATAA GCAGCTGCGG 
GCGGACATGG AGGACCTCAT GAGCTCCAAG GACGATGTGG GCAAGAACGT TCATGAACTT 
GAGAAATCCA AACGGGCCCT AGAGCAGCAG GTGGAGGAAA TGAGGACCCA ACTGGAGGAG 
CTGGAAGATG AGCTCCAGGC TACAGAAGAC GCCAAGCTTC GCCTAGAGGT CAACATGCAA 
GCCATGAAAG CCCAGTTTGA GAGAGACTTG CAGACCAGGG ATGAGCAGAA TGAGGAAAAG 
AAGCGGCTGC TCATTAAACA GGTGCGGGAG CTTGAGGCAG AGCTGGAGGA TGAGCGGAAA 
CAGCGGGCCC TGGCTGTTGC CTCGAAGAAG AAAATGGAAA TAGACCTGAA GGACCTGGAG 
GCTCAAATCG AGGCAGCTAA CAAAGCAAGG GATGAAGTGA TCAAGCAGCT CCGCAAACTC 
CAGGCTCAGA TGAAGGACTA CCAGCGTGAA TTAGAAGAAG CTCGTGCATC CCGGGATGAG 
ATTTTTGCTC AATCCAAAGA GAGTGAGAAG AAACTGAAAA GCCTGGAAGC AGAAATCCTT 
CAGTTGCAGG AGGAACTTGC CTCATCCGAG CGAGCCCGCC GACATGCTGA GCAGGAGAGA 
GACGAGCTGG CGGACGAGAT TGCCAATAGC GCCTCCGGCA AGTCTGCACT CCTGGATGAG 
AAGCGGCGTC TGGAGGCTCG GATCGCACAG CTAGAGGAGG AGCTGGAGGA GGAGCAGAGC 
AACATGGAGC TGCTCAATGA CCGCTTCCGC AAGACCACAC TGCAGGTGGA CACGCTGAAC 
ACTGAGCTGG CGGCCGAGCG CAGCGCTGCC CAGAAGAGCG ATAATGCGCG CCAGCAGCTG 
GAGCGGCAGA ACAAGGAGTT GAAGGCCAAG CTGCAGGAGC TGGAAGGCGC TGTCAAGTCC 
AAGTTCAAGG CCACCATCTC TGCTCTAGAA GCCAAGATTG GGCAGCTGGA GGAGCAGCTT 
GAGCAGGAAG CCAAGGAAAG AGCCGCTGCC AATAAACTAG TCCGTCGTAC CGAGAAGAAG 
CTGAAAGAAA TCTTCATGCA GGTTGAAGAT GAACGCCGCC ATGCTGACCA GTATAAAGAA 
CAGATGGAGA AGGCCAATGC CAGGATGAAG CAGCTCAAGC GGCAGCTGGA GGAAGCAGAG 
GAAGAAGCTA CACGTGCCAA CGCATCTCGG CGCAAACTCC AGCGGGAACT GGATGATGCC 
ACTGAGGCCA ACGAGGGCCT GAGCCGCGAG GTCAGCACCC TGAAGAACCG GCTGAGGCGA 
GGCGGCCCGA TCAGCTTCTC CTCCAGCCGA TCAGGCCGGC GCCAGCTGCA CATTGAGGGC 
GCGTCCCTGG AGCTGTCGGA CGACGACACA GAAAGTAAGA CCAGTGACAT CAACGAGACA 
CAGCCGCCGC AGTCCGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH10 ( XM_015458672.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314158.1 Bos taurus myosin, heavy chain 10, non-muscle (MYH10), transcript variant X6, mRNA.

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CloneID	OBa93841
Clone ID Related Accession (Same CDS sequence)	XM_015458672.1
Accession Version	XM_015458672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myosin-10 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG CCATGCGGGG CGAATCAGGT GCCGGGAAGA CAGAAAACAC TAAGAAAGTC 
ATTCAGTACC TTGCTCATGT TGCTTCTTCA CACAAAGGAC GAAAGGACCA TAATATTCCT 
CAGGAATCAC CTAAACCAGT GAAACACCAG GGGGAACTTG AACGGCAGCT TTTGCAAGCA 
AATCCAATCC TGGAATCATT TGGAAACGCA AAGACTGTGA AAAATGATAA CTCATCTCGT 
TTTGGCAAGT TTATTCGGAT CAACTTTGAT GTAACTGGCT ATATCGTTGG GGCCAACATT 
GAAACATACC TTCTGGAAAA ATCTCGTGCT GTTCGTCAAG CAAAAGATGA GCGTACTTTT 
CATATCTTTT ACCAATTGTT ATCTGGAGCA GGAGAACATC TGAAGTCTGA TTTGCTCCTT 
GAAGGATTTA ATAACTACAG ATTTCTCTCC AATGGCTATA TTCCTATTCC GGGACAACAA 
GACAAAGATA ACTTCCAGGA GACAATGGAG GCAATGCATA TAATGGGCTT CTCCCATGAA 
GAGATTCTGT CAATGCTTAA AGTGGTATCT TCAGTGCTTC AGTTTGGAAA TATTTCTTTC 
AAAAAGGAGC GAAACACTGA TCAAGCCTCC ATGCCGGAAA ATACAGTTGC ACAGAAGCTC 
TGCCATCTTC TTGGCATGAA TGTGATGGAG TTCACTCGGG CCATTCTCAC CCCCCGGATC 
AAGGTCGGCC GAGACTACGT GCAGAAAGCC CAGACCAAAG AACAAGCAGA TTTTGCAGTA 
GAAGCTTTGG CAAAAGCTAC CTATGAGCGG CTCTTCCGCT GGCTCGTTCA CCGCATCAAT 
AAAGCTCTGG ACAGGACCAA ACGGCAAGGG GCCTCCTTCA TTGGGATCCT GGACATCGCT 
GGATTTGAGA TTTTTGAGCT GAACTCATTC GAGCAACTGT GCATCAACTA CACCAACGAG 
AAGCTGCAGC AGCTCTTCAA CCACACCATG TTCATCCTGG AGCAGGAGGA GTACCAGCGC 
GAGGGCATCG AGTGGAACTT CATCGACTTT GGCCTCGACC TGCAGCCCTG CATTGACCTC 
ATCGAGAGAC CTGCTAACCC TCCTGGCGTG CTGGCCCTTC TGGATGAAGA GTGCTGGTTC 
CCTAAAGCCA CAGATAAGAC CTTTGTTGAA AAGCTCGTTC AAGAGCAAGG GTCCCACTCC 
AAGTTTCAGA AACCACGTCA ATTGAAAGAC AAAGCTGATT TTTGCATTAT CCATTATGCA 
GGGAAGGTGG ACTACAAGGC AGACGAGTGG CTGATGAAGA ACATGGACCC CCTGAATGAC 
AACGTGGCCA CCCTCCTGCA CCAGTCCTCT GACAGATTCG TGGCAGAGCT CTGGAAGGAT 
GAGATTCAGA ATATTCAGAG AGCTTCTTTC TATGACAGTG TTTCTGGTCT TCATGAGCCA 
CCAGTGGACC GGATCGTGGG TCTGGATCAG GTCACGGGGA TGACGGAGAC GGCATTCGGC 
TCCGCCTATA AAACCAAGAA GGGCATGTTC CGCACTGTCG GGCAGCTCTA CAAAGAATCG 
CTCACCAAGC TGATGGCGAC GCTCCGGAAC ACCAACCCGA ACTTCGTGCG CTGCATCATC 
CCAAATCACG AGAAGCGGGC TGGGAAACTG GACCCACACC TGGTGCTGGA TCAGCTCCGC 
TGCAATGGCG TCCTGGAAGG GATCCGCATC TGCCGCCAGG GCTTCCCCAA CCGGATCGTT 
TTCCAGGAAT TCAGACAGAG ATACGAGATC CTCACTCCAA ATGCTATCCC TAAGGGTTTC 
ATGGATGGCA AACAGGCTTG TGAACGAATG ATCCGGGCTT TAGAATTGGA CCCAAACTTG 
TACAGAATTG GACAGAGCAA GATATTTTTC AGAGCTGGAG TCTTGGCACA CTTAGAGGAA 
GAACGAGATT TAAAAATTAC CGACATCATT ATCTTCTTCC AGGCTGTTTG CAGGGGTTAT 
TTAGCCAGAA AGGCCTTCGC CAAGAAGCAG CAGCAGCTGA GTGCCCTGAA GGTGCTGCAG 
CGCAACTGTG CCGCGTACCT GAAGCTGCGG CACTGGCAGT GGTGGCGCGT CTTCACCAAG 
GTGAAACCAC TCCTACAAGT CACTCGTCAA GAGGAGGAGC TCCAGGCCAA AGATGAAGAG 
CTGCTGAAGG TGAAAGAGAA GCAGACGAAG GTGGAAGGGG AGCTGGAGGA GATGGAGAGG 
AAGCACCAAC AGCTGCTGGA GGAGAAGAAT ATCCTGGCAG AGCAGCTGCA GGCGGAGACC 
GAGCTCTTTG CCGAAGCAGA GGAGATGAGG GCCAGACTTG CCGCTAAGAA GCAGGAATTA 
GAAGAGATTC TCCATGACTT GGAATCTAGG GTAGAAGAAG AAGAAGAAAG AAACCAAATC 
CTCCAAAATG AAAAGAAGAA AATGCAAGCA CACATTCAGG ACCTGGAGGA GCAGCTAGAT 
GAGGAGGAGG GGGCTCGCCA GAAGCTGCAG CTGGAGAAGG TGACGGCGGA GGCCAAGATC 
AAGAAGATGG AAGAAGAGAT TCTGCTTCTG GAGGACCAGA ACTCCAAGTT TATCAAAGAA 
AAGAAACTCA TGGAAGATCG CATTGCAGAA TGTTCCTCTC AGCTGGCTGA AGAGGAAGAA 
AAGGCAAAAA ACTTGGCCAA AATCAGGAAT AAGCAAGAAG TAATGATCTC GGATTTAGAA 
GAGCGCTTAA AGAAGGAGGA GAAGACGCGC CAGGAGCTGG AGAAGGCCAA AAGGAAACTG 
GACGGGGAGA CCACGGACCT GCAAGACCAG ATCGCCGAGC TCCAGGCGCA GATTGACGAG 
CTTAAGATCC AGGTGGCCAA GAAAGAGGAG GAGCTGCAGG GTGCCCTGGC CAGGGGTGAC 
GACGAGACGC TACACAAGAA CAACGCACTA AAAGTCGTGC GAGAGCTGCA GGCCCAGATC 
GCCGAGCTGC AGGAGGACTT TGAATCTGAG AAGGCCTCGC GGAACAAAGC AGAGAAGCAG 
AAGAGGGACT TGAGTGAGGA GCTGGAGGCA CTCAAGACGG AGCTGGAGGA CACGCTGGAC 
ACCACGGCGG CCCAGCAGGA ACTGCGTACA AAACGAGAAC AAGAAGTGGC GGAGCTGAAG 
AAGGCTCTCG AGGAGGAAAC GAAGAGCCAC GAGGCTCAGA TCCAGGACAT GAGGCAGAGG 
CATGCGACCG CCTTGGAGGA GCTGTCGGAG CAGCTGGAGC AGGCCAAGAG GTTCAAAGCA 
AATCTGGAAA AGAACAAGCA GGGCCTGGAG ACAGACAACA AGGAGCTGGC GTGCGAGGTC 
AAGGTGCTGC AGCAGGTCAA GGCTGAGTCC GAGCACAAGA GGAAGAAGCT GGACGCCCAG 
GTCCAGGAGC TCCACGCCAA GGTCTCAGAG GGAGACAGAC TCAGGGTGGA GCTGGCAGAG 
AAAGCCAATA AGTTACAGAA TGAATTGGAT AATGTCTCAA CTCTTCTAGA AGAAGCAGAG 
AAGAAGGGTA TTAAATTTGC TAAGGATGCA GCTGGTCTGG AGTCTCAGCT CCAAGACACA 
CAGGAGCTTC TTCAGGAGGA GACGCGCCAG AAACTGAACC TGAGCAGTCG GATCCGGCAG 
CTGGAGGAGG AGAGGAGCAG CCTCCAGGAG CAGCAGGAGG AAGAGGAGGA GGCCAGGAGG 
AGCCTGGAGA AGCAGCTGCA GGCCCTGCAG GCCCAGTTGA CGGATACCAA GAAGAAAGTA 
GATGATGACC TGGGGACAAT TGAAAATTTG GAAGAAGCCA AGAAGAAGCT CCTCAAGGAT 
GTGGAGGTGC TGAGCCAGCG CCTGGAGGAG AAGGCCCTGG CCTATGACAA GCTGGAGAAG 
ACCAAGACCC GCCTGCAGCA GGAGCTGGAT GACCTGCTGG TAGACCTGGA CCACCAGCGC 
CAGATCGTCT CCAACTTGGA GAAGAAGCAG AAGAAGTTTG ATCAGCTGTT AGCGGAAGAG 
AAGAACATCT CTGCCCGCTA CGCAGAAGAG CGCGACCGGG CAGAGGCAGA GGCCCGAGAG 
AAGGAGACCA AAGCCCTGTC CCTGGCCCGC GCCCTGGAGG AGGCCCTGGA GGCCAGGGAG 
GAGGCTGAGC GACAGAATAA GCAGCTGCGG GCGGACATGG AGGACCTCAT GAGCTCCAAG 
GACGATGTGG GCAAGAACGT TCATGAACTT GAGAAATCCA AACGGGCCCT AGAGCAGCAG 
GTGGAGGAAA TGAGGACCCA ACTGGAGGAG CTGGAAGATG AGCTCCAGGC TACAGAAGAC 
GCCAAGCTTC GCCTAGAGGT CAACATGCAA GCCATGAAAG CCCAGTTTGA GAGAGACTTG 
CAGACCAGGG ATGAGCAGAA TGAGGAAAAG AAGCGGCTGC TCATTAAACA GGTGCGGGAG 
CTTGAGGCAG AGCTGGAGGA TGAGCGGAAA CAGCGGGCCC TGGCTGTTGC CTCGAAGAAG 
AAAATGGAAA TAGACCTGAA GGACCTGGAG GCTCAAATCG AGGCAGCTAA CAAAGCAAGG 
GATGAAGTGA TCAAGCAGCT CCGCAAACTC CAGGCTCAGA TGAAGGACTA CCAGCGTGAA 
TTAGAAGAAG CTCGTGCATC CCGGGATGAG ATTTTTGCTC AATCCAAAGA GAGTGAGAAG 
AAACTGAAAA GCCTGGAAGC AGAAATCCTT CAGTTGCAGG AGGAACTTGC CTCATCCGAG 
CGAGCCCGCC GACATGCTGA GCAGGAGAGA GACGAGCTGG CGGACGAGAT TGCCAATAGC 
GCCTCCGGCA AGTCTGCACT CCTGGATGAG AAGCGGCGTC TGGAGGCTCG GATCGCACAG 
CTAGAGGAGG AGCTGGAGGA GGAGCAGAGC AACATGGAGC TGCTCAATGA CCGCTTCCGC 
AAGACCACAC TGCAGGTGGA CACGCTGAAC ACTGAGCTGG CGGCCGAGCG CAGCGCTGCC 
CAGAAGAGCG ATAATGCGCG CCAGCAGCTG GAGCGGCAGA ACAAGGAGTT GAAGGCCAAG 
CTGCAGGAGC TGGAAGGCGC TGTCAAGTCC AAGTTCAAGG CCACCATCTC TGCTCTAGAA 
GCCAAGATTG GGCAGCTGGA GGAGCAGCTT GAGCAGGAAG CCAAGGAAAG AGCCGCTGCC 
AATAAACTAG TCCGTCGTAC CGAGAAGAAG CTGAAAGAAA TCTTCATGCA GGTTGAAGAT 
GAACGCCGCC ATGCTGACCA GTATAAAGAA CAGATGGAGA AGGCCAATGC CAGGATGAAG 
CAGCTCAAGC GGCAGCTGGA GGAAGCAGAG GAAGAAGCTA CACGTGCCAA CGCATCTCGG 
CGCAAACTCC AGCGGGAACT GGATGATGCC ACTGAGGCCA ACGAGGGCCT GAGCCGCGAG 
GTCAGCACCC TGAAGAACCG GCTGAGGCGA GGCGGCCCGA TCAGCTTCTC CTCCAGCCGA 
TCAGGCCGGC GCCAGCTGCA CATTGAGGGC GCGTCCCTGG AGCTGTCGGA CGACGACACA 
GAAAGTAAGA CCAGTGACAT CAACGAGACA CAGCCGCCGC AGTCCGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314158.1
CDS	525..6035
Translation	MTEAMRGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPQESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDEIQNIQRASFYDSVSGLHEPPVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKEKQTKVEGELEEMERKHQQLLEEKNILAEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKIQVAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETKSHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKANKLQNELDNVSTLLEEAEKKGIKFAKDAAGLESQLQDTQELLQEETRQKLNLSSRIRQLEEERSSLQEQQEEEEEARRSLEKQLQALQAQLTDTKKKVDDDLGTIENLEEAKKKLLKDVEVLSQRLEEKALAYDKLEKTKTRLQQELDDLLVDLDHQRQIVSNLEKKQKKFDQLLAEEKNISARYAEERDRAEAEAREKETKALSLARALEEALEAREEAERQNKQLRADMEDLMSSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLIKQVRELEAELEDERKQRALAVASKKKMEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNTELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDTESKTSDINETQPPQSE

Target ORF information:

RefSeq Version	XM_015458672.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin, heavy chain 10, non-muscle (MYH10), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458672.1

ORF Insert Sequence:

ATGACGGAAG CCATGCGGGG CGAATCAGGT GCCGGGAAGA CAGAAAACAC TAAGAAAGTC 
ATTCAGTACC TTGCTCATGT TGCTTCTTCA CACAAAGGAC GAAAGGACCA TAATATTCCT 
CAGGAATCAC CTAAACCAGT GAAACACCAG GGGGAACTTG AACGGCAGCT TTTGCAAGCA 
AATCCAATCC TGGAATCATT TGGAAACGCA AAGACTGTGA AAAATGATAA CTCATCTCGT 
TTTGGCAAGT TTATTCGGAT CAACTTTGAT GTAACTGGCT ATATCGTTGG GGCCAACATT 
GAAACATACC TTCTGGAAAA ATCTCGTGCT GTTCGTCAAG CAAAAGATGA GCGTACTTTT 
CATATCTTTT ACCAATTGTT ATCTGGAGCA GGAGAACATC TGAAGTCTGA TTTGCTCCTT 
GAAGGATTTA ATAACTACAG ATTTCTCTCC AATGGCTATA TTCCTATTCC GGGACAACAA 
GACAAAGATA ACTTCCAGGA GACAATGGAG GCAATGCATA TAATGGGCTT CTCCCATGAA 
GAGATTCTGT CAATGCTTAA AGTGGTATCT TCAGTGCTTC AGTTTGGAAA TATTTCTTTC 
AAAAAGGAGC GAAACACTGA TCAAGCCTCC ATGCCGGAAA ATACAGTTGC ACAGAAGCTC 
TGCCATCTTC TTGGCATGAA TGTGATGGAG TTCACTCGGG CCATTCTCAC CCCCCGGATC 
AAGGTCGGCC GAGACTACGT GCAGAAAGCC CAGACCAAAG AACAAGCAGA TTTTGCAGTA 
GAAGCTTTGG CAAAAGCTAC CTATGAGCGG CTCTTCCGCT GGCTCGTTCA CCGCATCAAT 
AAAGCTCTGG ACAGGACCAA ACGGCAAGGG GCCTCCTTCA TTGGGATCCT GGACATCGCT 
GGATTTGAGA TTTTTGAGCT GAACTCATTC GAGCAACTGT GCATCAACTA CACCAACGAG 
AAGCTGCAGC AGCTCTTCAA CCACACCATG TTCATCCTGG AGCAGGAGGA GTACCAGCGC 
GAGGGCATCG AGTGGAACTT CATCGACTTT GGCCTCGACC TGCAGCCCTG CATTGACCTC 
ATCGAGAGAC CTGCTAACCC TCCTGGCGTG CTGGCCCTTC TGGATGAAGA GTGCTGGTTC 
CCTAAAGCCA CAGATAAGAC CTTTGTTGAA AAGCTCGTTC AAGAGCAAGG GTCCCACTCC 
AAGTTTCAGA AACCACGTCA ATTGAAAGAC AAAGCTGATT TTTGCATTAT CCATTATGCA 
GGGAAGGTGG ACTACAAGGC AGACGAGTGG CTGATGAAGA ACATGGACCC CCTGAATGAC 
AACGTGGCCA CCCTCCTGCA CCAGTCCTCT GACAGATTCG TGGCAGAGCT CTGGAAGGAT 
GAGATTCAGA ATATTCAGAG AGCTTCTTTC TATGACAGTG TTTCTGGTCT TCATGAGCCA 
CCAGTGGACC GGATCGTGGG TCTGGATCAG GTCACGGGGA TGACGGAGAC GGCATTCGGC 
TCCGCCTATA AAACCAAGAA GGGCATGTTC CGCACTGTCG GGCAGCTCTA CAAAGAATCG 
CTCACCAAGC TGATGGCGAC GCTCCGGAAC ACCAACCCGA ACTTCGTGCG CTGCATCATC 
CCAAATCACG AGAAGCGGGC TGGGAAACTG GACCCACACC TGGTGCTGGA TCAGCTCCGC 
TGCAATGGCG TCCTGGAAGG GATCCGCATC TGCCGCCAGG GCTTCCCCAA CCGGATCGTT 
TTCCAGGAAT TCAGACAGAG ATACGAGATC CTCACTCCAA ATGCTATCCC TAAGGGTTTC 
ATGGATGGCA AACAGGCTTG TGAACGAATG ATCCGGGCTT TAGAATTGGA CCCAAACTTG 
TACAGAATTG GACAGAGCAA GATATTTTTC AGAGCTGGAG TCTTGGCACA CTTAGAGGAA 
GAACGAGATT TAAAAATTAC CGACATCATT ATCTTCTTCC AGGCTGTTTG CAGGGGTTAT 
TTAGCCAGAA AGGCCTTCGC CAAGAAGCAG CAGCAGCTGA GTGCCCTGAA GGTGCTGCAG 
CGCAACTGTG CCGCGTACCT GAAGCTGCGG CACTGGCAGT GGTGGCGCGT CTTCACCAAG 
GTGAAACCAC TCCTACAAGT CACTCGTCAA GAGGAGGAGC TCCAGGCCAA AGATGAAGAG 
CTGCTGAAGG TGAAAGAGAA GCAGACGAAG GTGGAAGGGG AGCTGGAGGA GATGGAGAGG 
AAGCACCAAC AGCTGCTGGA GGAGAAGAAT ATCCTGGCAG AGCAGCTGCA GGCGGAGACC 
GAGCTCTTTG CCGAAGCAGA GGAGATGAGG GCCAGACTTG CCGCTAAGAA GCAGGAATTA 
GAAGAGATTC TCCATGACTT GGAATCTAGG GTAGAAGAAG AAGAAGAAAG AAACCAAATC 
CTCCAAAATG AAAAGAAGAA AATGCAAGCA CACATTCAGG ACCTGGAGGA GCAGCTAGAT 
GAGGAGGAGG GGGCTCGCCA GAAGCTGCAG CTGGAGAAGG TGACGGCGGA GGCCAAGATC 
AAGAAGATGG AAGAAGAGAT TCTGCTTCTG GAGGACCAGA ACTCCAAGTT TATCAAAGAA 
AAGAAACTCA TGGAAGATCG CATTGCAGAA TGTTCCTCTC AGCTGGCTGA AGAGGAAGAA 
AAGGCAAAAA ACTTGGCCAA AATCAGGAAT AAGCAAGAAG TAATGATCTC GGATTTAGAA 
GAGCGCTTAA AGAAGGAGGA GAAGACGCGC CAGGAGCTGG AGAAGGCCAA AAGGAAACTG 
GACGGGGAGA CCACGGACCT GCAAGACCAG ATCGCCGAGC TCCAGGCGCA GATTGACGAG 
CTTAAGATCC AGGTGGCCAA GAAAGAGGAG GAGCTGCAGG GTGCCCTGGC CAGGGGTGAC 
GACGAGACGC TACACAAGAA CAACGCACTA AAAGTCGTGC GAGAGCTGCA GGCCCAGATC 
GCCGAGCTGC AGGAGGACTT TGAATCTGAG AAGGCCTCGC GGAACAAAGC AGAGAAGCAG 
AAGAGGGACT TGAGTGAGGA GCTGGAGGCA CTCAAGACGG AGCTGGAGGA CACGCTGGAC 
ACCACGGCGG CCCAGCAGGA ACTGCGTACA AAACGAGAAC AAGAAGTGGC GGAGCTGAAG 
AAGGCTCTCG AGGAGGAAAC GAAGAGCCAC GAGGCTCAGA TCCAGGACAT GAGGCAGAGG 
CATGCGACCG CCTTGGAGGA GCTGTCGGAG CAGCTGGAGC AGGCCAAGAG GTTCAAAGCA 
AATCTGGAAA AGAACAAGCA GGGCCTGGAG ACAGACAACA AGGAGCTGGC GTGCGAGGTC 
AAGGTGCTGC AGCAGGTCAA GGCTGAGTCC GAGCACAAGA GGAAGAAGCT GGACGCCCAG 
GTCCAGGAGC TCCACGCCAA GGTCTCAGAG GGAGACAGAC TCAGGGTGGA GCTGGCAGAG 
AAAGCCAATA AGTTACAGAA TGAATTGGAT AATGTCTCAA CTCTTCTAGA AGAAGCAGAG 
AAGAAGGGTA TTAAATTTGC TAAGGATGCA GCTGGTCTGG AGTCTCAGCT CCAAGACACA 
CAGGAGCTTC TTCAGGAGGA GACGCGCCAG AAACTGAACC TGAGCAGTCG GATCCGGCAG 
CTGGAGGAGG AGAGGAGCAG CCTCCAGGAG CAGCAGGAGG AAGAGGAGGA GGCCAGGAGG 
AGCCTGGAGA AGCAGCTGCA GGCCCTGCAG GCCCAGTTGA CGGATACCAA GAAGAAAGTA 
GATGATGACC TGGGGACAAT TGAAAATTTG GAAGAAGCCA AGAAGAAGCT CCTCAAGGAT 
GTGGAGGTGC TGAGCCAGCG CCTGGAGGAG AAGGCCCTGG CCTATGACAA GCTGGAGAAG 
ACCAAGACCC GCCTGCAGCA GGAGCTGGAT GACCTGCTGG TAGACCTGGA CCACCAGCGC 
CAGATCGTCT CCAACTTGGA GAAGAAGCAG AAGAAGTTTG ATCAGCTGTT AGCGGAAGAG 
AAGAACATCT CTGCCCGCTA CGCAGAAGAG CGCGACCGGG CAGAGGCAGA GGCCCGAGAG 
AAGGAGACCA AAGCCCTGTC CCTGGCCCGC GCCCTGGAGG AGGCCCTGGA GGCCAGGGAG 
GAGGCTGAGC GACAGAATAA GCAGCTGCGG GCGGACATGG AGGACCTCAT GAGCTCCAAG 
GACGATGTGG GCAAGAACGT TCATGAACTT GAGAAATCCA AACGGGCCCT AGAGCAGCAG 
GTGGAGGAAA TGAGGACCCA ACTGGAGGAG CTGGAAGATG AGCTCCAGGC TACAGAAGAC 
GCCAAGCTTC GCCTAGAGGT CAACATGCAA GCCATGAAAG CCCAGTTTGA GAGAGACTTG 
CAGACCAGGG ATGAGCAGAA TGAGGAAAAG AAGCGGCTGC TCATTAAACA GGTGCGGGAG 
CTTGAGGCAG AGCTGGAGGA TGAGCGGAAA CAGCGGGCCC TGGCTGTTGC CTCGAAGAAG 
AAAATGGAAA TAGACCTGAA GGACCTGGAG GCTCAAATCG AGGCAGCTAA CAAAGCAAGG 
GATGAAGTGA TCAAGCAGCT CCGCAAACTC CAGGCTCAGA TGAAGGACTA CCAGCGTGAA 
TTAGAAGAAG CTCGTGCATC CCGGGATGAG ATTTTTGCTC AATCCAAAGA GAGTGAGAAG 
AAACTGAAAA GCCTGGAAGC AGAAATCCTT CAGTTGCAGG AGGAACTTGC CTCATCCGAG 
CGAGCCCGCC GACATGCTGA GCAGGAGAGA GACGAGCTGG CGGACGAGAT TGCCAATAGC 
GCCTCCGGCA AGTCTGCACT CCTGGATGAG AAGCGGCGTC TGGAGGCTCG GATCGCACAG 
CTAGAGGAGG AGCTGGAGGA GGAGCAGAGC AACATGGAGC TGCTCAATGA CCGCTTCCGC 
AAGACCACAC TGCAGGTGGA CACGCTGAAC ACTGAGCTGG CGGCCGAGCG CAGCGCTGCC 
CAGAAGAGCG ATAATGCGCG CCAGCAGCTG GAGCGGCAGA ACAAGGAGTT GAAGGCCAAG 
CTGCAGGAGC TGGAAGGCGC TGTCAAGTCC AAGTTCAAGG CCACCATCTC TGCTCTAGAA 
GCCAAGATTG GGCAGCTGGA GGAGCAGCTT GAGCAGGAAG CCAAGGAAAG AGCCGCTGCC 
AATAAACTAG TCCGTCGTAC CGAGAAGAAG CTGAAAGAAA TCTTCATGCA GGTTGAAGAT 
GAACGCCGCC ATGCTGACCA GTATAAAGAA CAGATGGAGA AGGCCAATGC CAGGATGAAG 
CAGCTCAAGC GGCAGCTGGA GGAAGCAGAG GAAGAAGCTA CACGTGCCAA CGCATCTCGG 
CGCAAACTCC AGCGGGAACT GGATGATGCC ACTGAGGCCA ACGAGGGCCT GAGCCGCGAG 
GTCAGCACCC TGAAGAACCG GCTGAGGCGA GGCGGCCCGA TCAGCTTCTC CTCCAGCCGA 
TCAGGCCGGC GCCAGCTGCA CATTGAGGGC GCGTCCCTGG AGCTGTCGGA CGACGACACA 
GAAAGTAAGA CCAGTGACAT CAACGAGACA CAGCCGCCGC AGTCCGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MYH10
Entrez Gene ID	317655
Full Name	myosin heavy chain 10
General protein information	Preferred Names myosin heavy chain 10 Names myosin-10 NMMHC II-b NMMHC-B NMMHC-IIB cellular myosin heavy chain, type B myosin heavy chain, non-muscle IIb myosin, heavy polypeptide 10, non-muscle non-muscle myosin heavy chain B non-muscle myosin heavy chain IIb nonmuscle myosin heavy chain B
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	MYH10	XP_001118181.2
Rattus norvegicus (Norway rat)	Myh10	NP_113708.1
Gallus gallus (chicken)	MYH10	NP_990805.1
Pan troglodytes (chimpanzee)	MYH10	XP_001166541.2
Homo sapiens (human)	MYH10	NP_001242941.1
Canis lupus familiaris (dog)	MYH10	XP_005620092.1
Caenorhabditis elegans (roundworm)	nmy-1	NP_508504.2
Xenopus tropicalis (tropical clawed frog)	LOC100493774	XP_004916274.1
Mus musculus (house mouse)	Myh10	NP_780469.1
Drosophila melanogaster (fruit fly)	zip	NP_523860.2
Bos taurus (cattle)	MYH10	NP_777259.1
Danio rerio (zebrafish)	myh10	XP_005165776.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007523	XP_308355.3

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MYH10 cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

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