ORF cDNA Clones and Custom Clones » Species Summary » Bos taurus » TMEM259 cDNA ORF clone

TMEM259 cDNA ORF clone, Bos taurus(cattle)

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Summary

Gene Symbol	TMEM259
Entrez Gene ID	514413
Full Name	transmembrane protein 259
Synonyms	C7H19orf6
General protein information	Preferred Names transmembrane protein 259 Names membralin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010807271.2	XP_010805573.1	membralin isoform X1
XM_010807272.2	XP_010805574.1	membralin isoform X2
XM_015464247.1	XP_015319733.1	membralin isoform X1
XM_024995105.1	XP_024850873.1	membralin isoform X1
XM_010807273.2	XP_010805575.1	membralin isoform X3
XM_015464248.1	XP_015319734.1	membralin isoform X2
XM_024995106.1	XP_024850874.1	membralin isoform X2
XM_015464249.1	XP_015319735.1	membralin isoform X3
XM_010807275.2	XP_010805577.1	membralin isoform X5
XM_024995108.1	XP_024850876.1	membralin isoform X4
XM_024995109.1	XP_024850877.1	membralin isoform X5
XM_010807274.2	XP_010805576.1	membralin isoform X4
XM_015464250.1	XP_015319736.1	membralin isoform X4
XM_024995107.1	XP_024850875.1	membralin isoform X3
XM_015464251.1	XP_015319737.1	membralin isoform X5

Homologies

Homo sapiens (human)	TMEM259	NP_001028198.1
Pan troglodytes (chimpanzee)	C19H19orf6	XP_003954544.1
Xenopus tropicalis (tropical clawed frog)	tmem259	NP_001072254.1
Bos taurus (cattle)	TMEM259	XP_002689215.2
Danio rerio (zebrafish)	tmem259	XP_700127.3
Canis lupus familiaris (dog)	C20H19orf6	XP_533963.2
Gallus gallus (chicken)	C19ORF6	XP_418222.2
Mus musculus (house mouse)	Tmem259	NP_001003949.2
Rattus norvegicus (Norway rat)	Mbrl	NP_001094356.1

The following TMEM259 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM259 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa48951	XM_015464247.1 Latest version!	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$342.30 $489.00
OBa48951	XM_010807271.2 Latest version!	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$342.30 $489.00
OBa48952	XM_010807272.2 Latest version!	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$342.30 $489.00
OBa48952	XM_015464248.1 Latest version!	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$342.30 $489.00
OBa48953	XM_010807273.2 Latest version!	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X3, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$342.30 $489.00
OBa48953	XM_015464249.1 Latest version!	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X3, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$342.30 $489.00
OBa48954	XM_010807274.2 Latest version!	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X4, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$342.30 $489.00
OBa48954	XM_015464250.1 Latest version!	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X4, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$342.30 $489.00
OBa48955	XM_010807275.2 Latest version!	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X5, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$342.30 $489.00
OBa48955	XM_015464251.1 Latest version!	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X5, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$342.30 $489.00
OBa236632	XM_024995105.1 Latest version!	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	14-16	$307.30 $439.00
OBa236633	XM_024995106.1 Latest version!	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	14-16	$307.30 $439.00
OBa236634	XM_024995107.1 Latest version!	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X3, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	14-16	$307.30 $439.00
OBa236635	XM_024995108.1 Latest version!	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X4, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	14-16	$307.30 $439.00
OBa236636	XM_024995109.1 Latest version!	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X5, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMEM259 ( XM_010807271.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805573.1 Bos taurus transmembrane protein 259 (TMEM259), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa48951
Clone ID Related Accession (Same CDS sequence)	XM_010807271.2 , XM_015464247.1
Accession Version	XM_010807271.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1836bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	membralin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807271.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTC CTGTTTGTGT CCTGTCCTGG GGGGCCCCCT GGGTGGGGGC CGGGGCTACG 
GTTGCCGACT TACGAGGGAA CTTGAATTCC TTGTCCACAG AAAAGGGTCT GAGGCAGGCC 
CTGTTCGTGC TCTTTGTCCT GGCCTACATC CACATCGTCT TCTCCCGCTC ACCCATCAAC 
TGCCTGGAGC ACGTGCGTGA CAAGTGGCCC CGTGAGGGCA TCCTGCGGGT AGAGGTGCAG 
CACAACTCGA GCCGCGCGCC CGTCTTCCTG CAGTTCTGTG ATGGTGGCCA TGGCAGCTTC 
CCTGGCCTGG CCATGGAGCC AGATGGCCTG GAGCTAGAGG AGGAGGAGGA GGAGGAGCTG 
ACCATGGACA TGTTCGGGAA TAGCTCCATC AAGTTTGAAC TGGACATCGA GCCCAAGGTG 
TTCAAGCCAC CAGGTGGCCC CAAGGCCTTA AATGACAGCC AGGAGTTCCC ATTCCCTGAG 
ACGCCCCCAA AAGCCTGGCC ACAAGACGAG TACATCGTGG AGTACTCGCT GGAGTATGGC 
TTCCTGAGGT TGTCGCAGGC CACACGCCAG CGGCTCAGCA TCCCCGTAAT GGTGGTCACG 
CTGGATCCCA CGCGGGACCA GTGCTTTGGT GACCGCTTCA GCCGCCTGCT GCTGGCTGAG 
TTCCTGGGCT ATGATGACAT CCTCATGTCC AGCGTGAAGG GCCTGGCGGA GAACGAGGAG 
AACAAAGGCT TCCTGAGGAA TGTGGTGTCC GGGGAGCACT ACCGCTTCGT GAGCATGTGG 
ATGGCCCGCA CGTCCTACCT GGCCGCCTTC GTCATCATGG TCATCTTTAC CCTGAGCGTG 
TCCATGTTGC TGCGCTACTC CCACCATCAG ATCTTCGTTT TCATTGGTGA GTGTCCCCTC 
TGGCCATCTC CCTTGCCCCT CCCCCATTCC GGGCGCCTCA CCCGGTCTGC CCGCCCGCCA 
CCCTCTCCCG CAGTGGACCT GCTGCAGATG CTGGAGATGA ACATGGCCAT CGCCTTCCCC 
GCAGCGCCCC TGCTGACCGT CATCCTGGCC CTCGTGGGAA TGGAGGCGAT CATGTCTGAG 
TTCTTCAACG ACACCACCAC GGCCTTCTAC ATCATCCTCA TCGTGTGGCT GGCTGACCAG 
TATGACGCCA TCTGCTGCCA CACCAACACC AGCAAGCGGC ACTGGCTTCG GTTCTTCTAC 
CTGTACCACT TTGCCTTCTA CGCTTACCAC TATCGCTTCA ACGGGCAGTA CAGCAGCCTG 
GCACTCGTCA CCTCCTGGCT CTTCATCCAG CATTCCATGA TCTACTTCTT CCACCACTAC 
GAACTACCTG CCATCCTGCA GCAGATCCGT GTCCAGGAGA TGCTGCTGCA GACACCACCA 
CTGGGCCCCG GCACCCCCAC AGCCCTGCCA GACGACCTGA ACAACAATGG AGGCATCCCA 
CCTATCACCC CCGACGCTGC CAGCCAGTCC CCTGCCCTGG GCCCTGGCTT GCCAGGTGCT 
GGCGGGGGCC CCGGGCCCAT GGCTGAGGCA CCTAGCTCCC TGGTGGCCGC AGCGGCCTCA 
GTGGCTGCAG CAGCCAGTGG TGACCTGGGT TGGATGGCAG AGACGGCTGC CATCATCACA 
GACGCCTCCT TCCTGTCTGG CCTGAGCGCC TCTCTCCTGG ACCGGCGGCC AGGCAGCCCC 
CTGAGCCCTA GTGGGGGGCT CCTACGGGCC CCCCAGGACA GTGCCCCTGC AAGCAACTCT 
CCAGGGTCTG ATGCAGTCTC TCAGACCACC GGCGGGGGCA GGCCCAACCC TGTGTCTACC 
GATCTAGTGG ACACACCGTC GGAGGTCGGC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805573.1
CDS	94..1929
Translation	MAGPVCVLSWGAPWVGAGATVADLRGNLNSLSTEKGLRQALFVLFVLAYIHIVFSRSPINCLEHVRDKWPREGILRVEVQHNSSRAPVFLQFCDGGHGSFPGLAMEPDGLELEEEEEEELTMDMFGNSSIKFELDIEPKVFKPPGGPKALNDSQEFPFPETPPKAWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLAEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIGECPLWPSPLPLPHSGRLTRSARPPPSPAVDLLQMLEMNMAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRVQEMLLQTPPLGPGTPTALPDDLNNNGGIPPITPDAASQSPALGPGLPGAGGGPGPMAEAPSSLVAAAASVAAAASGDLGWMAETAAIITDASFLSGLSASLLDRRPGSPLSPSGGLLRAPQDSAPASNSPGSDAVSQTTGGGRPNPVSTDLVDTPSEVGS

Target ORF information:

RefSeq Version	XM_010807271.2
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807271.2

ORF Insert Sequence:

ATGGCTGGTC CTGTTTGTGT CCTGTCCTGG GGGGCCCCCT GGGTGGGGGC CGGGGCTACG 
GTTGCCGACT TACGAGGGAA CTTGAATTCC TTGTCCACAG AAAAGGGTCT GAGGCAGGCC 
CTGTTCGTGC TCTTTGTCCT GGCCTACATC CACATCGTCT TCTCCCGCTC ACCCATCAAC 
TGCCTGGAGC ACGTGCGTGA CAAGTGGCCC CGTGAGGGCA TCCTGCGGGT AGAGGTGCAG 
CACAACTCGA GCCGCGCGCC CGTCTTCCTG CAGTTCTGTG ATGGTGGCCA TGGCAGCTTC 
CCTGGCCTGG CCATGGAGCC AGATGGCCTG GAGCTAGAGG AGGAGGAGGA GGAGGAGCTG 
ACCATGGACA TGTTCGGGAA TAGCTCCATC AAGTTTGAAC TGGACATCGA GCCCAAGGTG 
TTCAAGCCAC CAGGTGGCCC CAAGGCCTTA AATGACAGCC AGGAGTTCCC ATTCCCTGAG 
ACGCCCCCAA AAGCCTGGCC ACAAGACGAG TACATCGTGG AGTACTCGCT GGAGTATGGC 
TTCCTGAGGT TGTCGCAGGC CACACGCCAG CGGCTCAGCA TCCCCGTAAT GGTGGTCACG 
CTGGATCCCA CGCGGGACCA GTGCTTTGGT GACCGCTTCA GCCGCCTGCT GCTGGCTGAG 
TTCCTGGGCT ATGATGACAT CCTCATGTCC AGCGTGAAGG GCCTGGCGGA GAACGAGGAG 
AACAAAGGCT TCCTGAGGAA TGTGGTGTCC GGGGAGCACT ACCGCTTCGT GAGCATGTGG 
ATGGCCCGCA CGTCCTACCT GGCCGCCTTC GTCATCATGG TCATCTTTAC CCTGAGCGTG 
TCCATGTTGC TGCGCTACTC CCACCATCAG ATCTTCGTTT TCATTGGTGA GTGTCCCCTC 
TGGCCATCTC CCTTGCCCCT CCCCCATTCC GGGCGCCTCA CCCGGTCTGC CCGCCCGCCA 
CCCTCTCCCG CAGTGGACCT GCTGCAGATG CTGGAGATGA ACATGGCCAT CGCCTTCCCC 
GCAGCGCCCC TGCTGACCGT CATCCTGGCC CTCGTGGGAA TGGAGGCGAT CATGTCTGAG 
TTCTTCAACG ACACCACCAC GGCCTTCTAC ATCATCCTCA TCGTGTGGCT GGCTGACCAG 
TATGACGCCA TCTGCTGCCA CACCAACACC AGCAAGCGGC ACTGGCTTCG GTTCTTCTAC 
CTGTACCACT TTGCCTTCTA CGCTTACCAC TATCGCTTCA ACGGGCAGTA CAGCAGCCTG 
GCACTCGTCA CCTCCTGGCT CTTCATCCAG CATTCCATGA TCTACTTCTT CCACCACTAC 
GAACTACCTG CCATCCTGCA GCAGATCCGT GTCCAGGAGA TGCTGCTGCA GACACCACCA 
CTGGGCCCCG GCACCCCCAC AGCCCTGCCA GACGACCTGA ACAACAATGG AGGCATCCCA 
CCTATCACCC CCGACGCTGC CAGCCAGTCC CCTGCCCTGG GCCCTGGCTT GCCAGGTGCT 
GGCGGGGGCC CCGGGCCCAT GGCTGAGGCA CCTAGCTCCC TGGTGGCCGC AGCGGCCTCA 
GTGGCTGCAG CAGCCAGTGG TGACCTGGGT TGGATGGCAG AGACGGCTGC CATCATCACA 
GACGCCTCCT TCCTGTCTGG CCTGAGCGCC TCTCTCCTGG ACCGGCGGCC AGGCAGCCCC 
CTGAGCCCTA GTGGGGGGCT CCTACGGGCC CCCCAGGACA GTGCCCCTGC AAGCAACTCT 
CCAGGGTCTG ATGCAGTCTC TCAGACCACC GGCGGGGGCA GGCCCAACCC TGTGTCTACC 
GATCTAGTGG ACACACCGTC GGAGGTCGGC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM259 ( XM_015464247.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319733.1 Bos taurus transmembrane protein 259 (TMEM259), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa48951
Clone ID Related Accession (Same CDS sequence)	XM_010807271.2 , XM_015464247.1
Accession Version	XM_015464247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1836bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	membralin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTC CTGTTTGTGT CCTGTCCTGG GGGGCCCCCT GGGTGGGGGC CGGGGCTACG 
GTTGCCGACT TACGAGGGAA CTTGAATTCC TTGTCCACAG AAAAGGGTCT GAGGCAGGCC 
CTGTTCGTGC TCTTTGTCCT GGCCTACATC CACATCGTCT TCTCCCGCTC ACCCATCAAC 
TGCCTGGAGC ACGTGCGTGA CAAGTGGCCC CGTGAGGGCA TCCTGCGGGT AGAGGTGCAG 
CACAACTCGA GCCGCGCGCC CGTCTTCCTG CAGTTCTGTG ATGGTGGCCA TGGCAGCTTC 
CCTGGCCTGG CCATGGAGCC AGATGGCCTG GAGCTAGAGG AGGAGGAGGA GGAGGAGCTG 
ACCATGGACA TGTTCGGGAA TAGCTCCATC AAGTTTGAAC TGGACATCGA GCCCAAGGTG 
TTCAAGCCAC CAGGTGGCCC CAAGGCCTTA AATGACAGCC AGGAGTTCCC ATTCCCTGAG 
ACGCCCCCAA AAGCCTGGCC ACAAGACGAG TACATCGTGG AGTACTCGCT GGAGTATGGC 
TTCCTGAGGT TGTCGCAGGC CACACGCCAG CGGCTCAGCA TCCCCGTAAT GGTGGTCACG 
CTGGATCCCA CGCGGGACCA GTGCTTTGGT GACCGCTTCA GCCGCCTGCT GCTGGCTGAG 
TTCCTGGGCT ATGATGACAT CCTCATGTCC AGCGTGAAGG GCCTGGCGGA GAACGAGGAG 
AACAAAGGCT TCCTGAGGAA TGTGGTGTCC GGGGAGCACT ACCGCTTCGT GAGCATGTGG 
ATGGCCCGCA CGTCCTACCT GGCCGCCTTC GTCATCATGG TCATCTTTAC CCTGAGCGTG 
TCCATGTTGC TGCGCTACTC CCACCATCAG ATCTTCGTTT TCATTGGTGA GTGTCCCCTC 
TGGCCATCTC CCTTGCCCCT CCCCCATTCC GGGCGCCTCA CCCGGTCTGC CCGCCCGCCA 
CCCTCTCCCG CAGTGGACCT GCTGCAGATG CTGGAGATGA ACATGGCCAT CGCCTTCCCC 
GCAGCGCCCC TGCTGACCGT CATCCTGGCC CTCGTGGGAA TGGAGGCGAT CATGTCTGAG 
TTCTTCAACG ACACCACCAC GGCCTTCTAC ATCATCCTCA TCGTGTGGCT GGCTGACCAG 
TATGACGCCA TCTGCTGCCA CACCAACACC AGCAAGCGGC ACTGGCTTCG GTTCTTCTAC 
CTGTACCACT TTGCCTTCTA CGCTTACCAC TATCGCTTCA ACGGGCAGTA CAGCAGCCTG 
GCACTCGTCA CCTCCTGGCT CTTCATCCAG CATTCCATGA TCTACTTCTT CCACCACTAC 
GAACTACCTG CCATCCTGCA GCAGATCCGT GTCCAGGAGA TGCTGCTGCA GACACCACCA 
CTGGGCCCCG GCACCCCCAC AGCCCTGCCA GACGACCTGA ACAACAATGG AGGCATCCCA 
CCTATCACCC CCGACGCTGC CAGCCAGTCC CCTGCCCTGG GCCCTGGCTT GCCAGGTGCT 
GGCGGGGGCC CCGGGCCCAT GGCTGAGGCA CCTAGCTCCC TGGTGGCCGC AGCGGCCTCA 
GTGGCTGCAG CAGCCAGTGG TGACCTGGGT TGGATGGCAG AGACGGCTGC CATCATCACA 
GACGCCTCCT TCCTGTCTGG CCTGAGCGCC TCTCTCCTGG ACCGGCGGCC AGGCAGCCCC 
CTGAGCCCTA GTGGGGGGCT CCTACGGGCC CCCCAGGACA GTGCCCCTGC AAGCAACTCT 
CCAGGGTCTG ATGCAGTCTC TCAGACCACC GGCGGGGGCA GGCCCAACCC TGTGTCTACC 
GATCTAGTGG ACACACCGTC GGAGGTCGGC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319733.1
CDS	94..1929
Translation	MAGPVCVLSWGAPWVGAGATVADLRGNLNSLSTEKGLRQALFVLFVLAYIHIVFSRSPINCLEHVRDKWPREGILRVEVQHNSSRAPVFLQFCDGGHGSFPGLAMEPDGLELEEEEEEELTMDMFGNSSIKFELDIEPKVFKPPGGPKALNDSQEFPFPETPPKAWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLAEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIGECPLWPSPLPLPHSGRLTRSARPPPSPAVDLLQMLEMNMAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRVQEMLLQTPPLGPGTPTALPDDLNNNGGIPPITPDAASQSPALGPGLPGAGGGPGPMAEAPSSLVAAAASVAAAASGDLGWMAETAAIITDASFLSGLSASLLDRRPGSPLSPSGGLLRAPQDSAPASNSPGSDAVSQTTGGGRPNPVSTDLVDTPSEVGS

Target ORF information:

RefSeq Version	XM_015464247.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464247.1

ORF Insert Sequence:

ATGGCTGGTC CTGTTTGTGT CCTGTCCTGG GGGGCCCCCT GGGTGGGGGC CGGGGCTACG 
GTTGCCGACT TACGAGGGAA CTTGAATTCC TTGTCCACAG AAAAGGGTCT GAGGCAGGCC 
CTGTTCGTGC TCTTTGTCCT GGCCTACATC CACATCGTCT TCTCCCGCTC ACCCATCAAC 
TGCCTGGAGC ACGTGCGTGA CAAGTGGCCC CGTGAGGGCA TCCTGCGGGT AGAGGTGCAG 
CACAACTCGA GCCGCGCGCC CGTCTTCCTG CAGTTCTGTG ATGGTGGCCA TGGCAGCTTC 
CCTGGCCTGG CCATGGAGCC AGATGGCCTG GAGCTAGAGG AGGAGGAGGA GGAGGAGCTG 
ACCATGGACA TGTTCGGGAA TAGCTCCATC AAGTTTGAAC TGGACATCGA GCCCAAGGTG 
TTCAAGCCAC CAGGTGGCCC CAAGGCCTTA AATGACAGCC AGGAGTTCCC ATTCCCTGAG 
ACGCCCCCAA AAGCCTGGCC ACAAGACGAG TACATCGTGG AGTACTCGCT GGAGTATGGC 
TTCCTGAGGT TGTCGCAGGC CACACGCCAG CGGCTCAGCA TCCCCGTAAT GGTGGTCACG 
CTGGATCCCA CGCGGGACCA GTGCTTTGGT GACCGCTTCA GCCGCCTGCT GCTGGCTGAG 
TTCCTGGGCT ATGATGACAT CCTCATGTCC AGCGTGAAGG GCCTGGCGGA GAACGAGGAG 
AACAAAGGCT TCCTGAGGAA TGTGGTGTCC GGGGAGCACT ACCGCTTCGT GAGCATGTGG 
ATGGCCCGCA CGTCCTACCT GGCCGCCTTC GTCATCATGG TCATCTTTAC CCTGAGCGTG 
TCCATGTTGC TGCGCTACTC CCACCATCAG ATCTTCGTTT TCATTGGTGA GTGTCCCCTC 
TGGCCATCTC CCTTGCCCCT CCCCCATTCC GGGCGCCTCA CCCGGTCTGC CCGCCCGCCA 
CCCTCTCCCG CAGTGGACCT GCTGCAGATG CTGGAGATGA ACATGGCCAT CGCCTTCCCC 
GCAGCGCCCC TGCTGACCGT CATCCTGGCC CTCGTGGGAA TGGAGGCGAT CATGTCTGAG 
TTCTTCAACG ACACCACCAC GGCCTTCTAC ATCATCCTCA TCGTGTGGCT GGCTGACCAG 
TATGACGCCA TCTGCTGCCA CACCAACACC AGCAAGCGGC ACTGGCTTCG GTTCTTCTAC 
CTGTACCACT TTGCCTTCTA CGCTTACCAC TATCGCTTCA ACGGGCAGTA CAGCAGCCTG 
GCACTCGTCA CCTCCTGGCT CTTCATCCAG CATTCCATGA TCTACTTCTT CCACCACTAC 
GAACTACCTG CCATCCTGCA GCAGATCCGT GTCCAGGAGA TGCTGCTGCA GACACCACCA 
CTGGGCCCCG GCACCCCCAC AGCCCTGCCA GACGACCTGA ACAACAATGG AGGCATCCCA 
CCTATCACCC CCGACGCTGC CAGCCAGTCC CCTGCCCTGG GCCCTGGCTT GCCAGGTGCT 
GGCGGGGGCC CCGGGCCCAT GGCTGAGGCA CCTAGCTCCC TGGTGGCCGC AGCGGCCTCA 
GTGGCTGCAG CAGCCAGTGG TGACCTGGGT TGGATGGCAG AGACGGCTGC CATCATCACA 
GACGCCTCCT TCCTGTCTGG CCTGAGCGCC TCTCTCCTGG ACCGGCGGCC AGGCAGCCCC 
CTGAGCCCTA GTGGGGGGCT CCTACGGGCC CCCCAGGACA GTGCCCCTGC AAGCAACTCT 
CCAGGGTCTG ATGCAGTCTC TCAGACCACC GGCGGGGGCA GGCCCAACCC TGTGTCTACC 
GATCTAGTGG ACACACCGTC GGAGGTCGGC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM259 ( XM_010807272.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805574.1 Bos taurus transmembrane protein 259 (TMEM259), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa48952
Clone ID Related Accession (Same CDS sequence)	XM_010807272.2 , XM_015464248.1
Accession Version	XM_010807272.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	membralin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807272.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTC CTGTTTGTGT CCTGTCCTGG GGGGCCCCCT GGGTGGGGGC CGGGGCTACG 
GTTGCCGACT TACGAGGGAA CTTGAATTCC TTGTCCACAG AAAAGGGTCT GAGGCAGGCC 
CTGTTCGTGC TCTTTGTCCT GGCCTACATC CACATCGTCT TCTCCCGCTC ACCCATCAAC 
TGCCTGGAGC ACGTGCGTGA CAAGTGGCCC CGTGAGGGCA TCCTGCGGGT AGAGGTGCAG 
CACAACTCGA GCCGCGCGCC CGTCTTCCTG CAGTTCTGTG ATGGTGGCCA TGGCAGCTTC 
CCTGGCCTGG CCATGGAGCC AGATGGCCTG GAGCTAGAGG AGGAGGAGGA GGAGGAGCTG 
ACCATGGACA TGTTCGGGAA TAGCTCCATC AAGTTTGAAC TGGACATCGA GCCCAAGGTG 
TTCAAGCCAC CAGGTGGCCC CAAGGCCTTA AATGACAGCC AGGAGTTCCC ATTCCCTGAG 
ACGCCCCCAA AAGCCTGGCC ACAAGACGAG TACATCGTGG AGTACTCGCT GGAGTATGGC 
TTCCTGAGGT TGTCGCAGGC CACACGCCAG CGGCTCAGCA TCCCCGTAAT GGTGGTCACG 
CTGGATCCCA CGCGGGACCA GTGCTTTGGT GACCGCTTCA GCCGCCTGCT GCTGGCTGAG 
TTCCTGGGCT ATGATGACAT CCTCATGTCC AGCGTGAAGG GCCTGGCGGA GAACGAGGAG 
AACAAAGGCT TCCTGAGGAA TGTGGTGTCC GGGGAGCACT ACCGCTTCGT GAGCATGTGG 
ATGGCCCGCA CGTCCTACCT GGCCGCCTTC GTCATCATGG TCATCTTTAC CCTGAGCGTG 
TCCATGTTGC TGCGCTACTC CCACCATCAG ATCTTCGTTT TCATTGTGGA CCTGCTGCAG 
ATGCTGGAGA TGAACATGGC CATCGCCTTC CCCGCAGCGC CCCTGCTGAC CGTCATCCTG 
GCCCTCGTGG GAATGGAGGC GATCATGTCT GAGTTCTTCA ACGACACCAC CACGGCCTTC 
TACATCATCC TCATCGTGTG GCTGGCTGAC CAGTATGACG CCATCTGCTG CCACACCAAC 
ACCAGCAAGC GGCACTGGCT TCGGTGGGCA CCAGTGGCCA TGTGGTCCGG GGTGGAGGCA 
GCCGGGTGGG CAGCACCTCA TTGTTCCCCT TGCAGGTTCT TCTACCTGTA CCACTTTGCC 
TTCTACGCTT ACCACTATCG CTTCAACGGG CAGTACAGCA GCCTGGCACT CGTCACCTCC 
TGGCTCTTCA TCCAGCATTC CATGATCTAC TTCTTCCACC ACTACGAACT ACCTGCCATC 
CTGCAGCAGA TCCGTGTCCA GGAGATGCTG CTGCAGACAC CACCACTGGG CCCCGGCACC 
CCCACAGCCC TGCCAGACGA CCTGAACAAC AATGGAGGCA TCCCACCTAT CACCCCCGAC 
GCTGCCAGCC AGTCCCCTGC CCTGGGCCCT GGCTTGCCAG GTGCTGGCGG GGGCCCCGGG 
CCCATGGCTG AGGCACCTAG CTCCCTGGTG GCCGCAGCGG CCTCAGTGGC TGCAGCAGCC 
AGTGGTGACC TGGGTTGGAT GGCAGAGACG GCTGCCATCA TCACAGACGC CTCCTTCCTG 
TCTGGCCTGA GCGCCTCTCT CCTGGACCGG CGGCCAGGCA GCCCCCTGAG CCCTAGTGGG 
GGGCTCCTAC GGGCCCCCCA GGACAGTGCC CCTGCAAGCA ACTCTCCAGG GTCTGATGCA 
GTCTCTCAGA CCACCGGCGG GGGCAGGCCC AACCCTGTGT CTACCGATCT AGTGGACACA 
CCGTCGGAGG TCGGCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805574.1
CDS	96..1916
Translation	MAGPVCVLSWGAPWVGAGATVADLRGNLNSLSTEKGLRQALFVLFVLAYIHIVFSRSPINCLEHVRDKWPREGILRVEVQHNSSRAPVFLQFCDGGHGSFPGLAMEPDGLELEEEEEEELTMDMFGNSSIKFELDIEPKVFKPPGGPKALNDSQEFPFPETPPKAWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLAEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIVDLLQMLEMNMAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRWAPVAMWSGVEAAGWAAPHCSPCRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRVQEMLLQTPPLGPGTPTALPDDLNNNGGIPPITPDAASQSPALGPGLPGAGGGPGPMAEAPSSLVAAAASVAAAASGDLGWMAETAAIITDASFLSGLSASLLDRRPGSPLSPSGGLLRAPQDSAPASNSPGSDAVSQTTGGGRPNPVSTDLVDTPSEVGS

Target ORF information:

RefSeq Version	XM_010807272.2
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807272.2

ORF Insert Sequence:

ATGGCTGGTC CTGTTTGTGT CCTGTCCTGG GGGGCCCCCT GGGTGGGGGC CGGGGCTACG 
GTTGCCGACT TACGAGGGAA CTTGAATTCC TTGTCCACAG AAAAGGGTCT GAGGCAGGCC 
CTGTTCGTGC TCTTTGTCCT GGCCTACATC CACATCGTCT TCTCCCGCTC ACCCATCAAC 
TGCCTGGAGC ACGTGCGTGA CAAGTGGCCC CGTGAGGGCA TCCTGCGGGT AGAGGTGCAG 
CACAACTCGA GCCGCGCGCC CGTCTTCCTG CAGTTCTGTG ATGGTGGCCA TGGCAGCTTC 
CCTGGCCTGG CCATGGAGCC AGATGGCCTG GAGCTAGAGG AGGAGGAGGA GGAGGAGCTG 
ACCATGGACA TGTTCGGGAA TAGCTCCATC AAGTTTGAAC TGGACATCGA GCCCAAGGTG 
TTCAAGCCAC CAGGTGGCCC CAAGGCCTTA AATGACAGCC AGGAGTTCCC ATTCCCTGAG 
ACGCCCCCAA AAGCCTGGCC ACAAGACGAG TACATCGTGG AGTACTCGCT GGAGTATGGC 
TTCCTGAGGT TGTCGCAGGC CACACGCCAG CGGCTCAGCA TCCCCGTAAT GGTGGTCACG 
CTGGATCCCA CGCGGGACCA GTGCTTTGGT GACCGCTTCA GCCGCCTGCT GCTGGCTGAG 
TTCCTGGGCT ATGATGACAT CCTCATGTCC AGCGTGAAGG GCCTGGCGGA GAACGAGGAG 
AACAAAGGCT TCCTGAGGAA TGTGGTGTCC GGGGAGCACT ACCGCTTCGT GAGCATGTGG 
ATGGCCCGCA CGTCCTACCT GGCCGCCTTC GTCATCATGG TCATCTTTAC CCTGAGCGTG 
TCCATGTTGC TGCGCTACTC CCACCATCAG ATCTTCGTTT TCATTGTGGA CCTGCTGCAG 
ATGCTGGAGA TGAACATGGC CATCGCCTTC CCCGCAGCGC CCCTGCTGAC CGTCATCCTG 
GCCCTCGTGG GAATGGAGGC GATCATGTCT GAGTTCTTCA ACGACACCAC CACGGCCTTC 
TACATCATCC TCATCGTGTG GCTGGCTGAC CAGTATGACG CCATCTGCTG CCACACCAAC 
ACCAGCAAGC GGCACTGGCT TCGGTGGGCA CCAGTGGCCA TGTGGTCCGG GGTGGAGGCA 
GCCGGGTGGG CAGCACCTCA TTGTTCCCCT TGCAGGTTCT TCTACCTGTA CCACTTTGCC 
TTCTACGCTT ACCACTATCG CTTCAACGGG CAGTACAGCA GCCTGGCACT CGTCACCTCC 
TGGCTCTTCA TCCAGCATTC CATGATCTAC TTCTTCCACC ACTACGAACT ACCTGCCATC 
CTGCAGCAGA TCCGTGTCCA GGAGATGCTG CTGCAGACAC CACCACTGGG CCCCGGCACC 
CCCACAGCCC TGCCAGACGA CCTGAACAAC AATGGAGGCA TCCCACCTAT CACCCCCGAC 
GCTGCCAGCC AGTCCCCTGC CCTGGGCCCT GGCTTGCCAG GTGCTGGCGG GGGCCCCGGG 
CCCATGGCTG AGGCACCTAG CTCCCTGGTG GCCGCAGCGG CCTCAGTGGC TGCAGCAGCC 
AGTGGTGACC TGGGTTGGAT GGCAGAGACG GCTGCCATCA TCACAGACGC CTCCTTCCTG 
TCTGGCCTGA GCGCCTCTCT CCTGGACCGG CGGCCAGGCA GCCCCCTGAG CCCTAGTGGG 
GGGCTCCTAC GGGCCCCCCA GGACAGTGCC CCTGCAAGCA ACTCTCCAGG GTCTGATGCA 
GTCTCTCAGA CCACCGGCGG GGGCAGGCCC AACCCTGTGT CTACCGATCT AGTGGACACA 
CCGTCGGAGG TCGGCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM259 ( XM_015464248.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319734.1 Bos taurus transmembrane protein 259 (TMEM259), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa48952
Clone ID Related Accession (Same CDS sequence)	XM_010807272.2 , XM_015464248.1
Accession Version	XM_015464248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	membralin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTC CTGTTTGTGT CCTGTCCTGG GGGGCCCCCT GGGTGGGGGC CGGGGCTACG 
GTTGCCGACT TACGAGGGAA CTTGAATTCC TTGTCCACAG AAAAGGGTCT GAGGCAGGCC 
CTGTTCGTGC TCTTTGTCCT GGCCTACATC CACATCGTCT TCTCCCGCTC ACCCATCAAC 
TGCCTGGAGC ACGTGCGTGA CAAGTGGCCC CGTGAGGGCA TCCTGCGGGT AGAGGTGCAG 
CACAACTCGA GCCGCGCGCC CGTCTTCCTG CAGTTCTGTG ATGGTGGCCA TGGCAGCTTC 
CCTGGCCTGG CCATGGAGCC AGATGGCCTG GAGCTAGAGG AGGAGGAGGA GGAGGAGCTG 
ACCATGGACA TGTTCGGGAA TAGCTCCATC AAGTTTGAAC TGGACATCGA GCCCAAGGTG 
TTCAAGCCAC CAGGTGGCCC CAAGGCCTTA AATGACAGCC AGGAGTTCCC ATTCCCTGAG 
ACGCCCCCAA AAGCCTGGCC ACAAGACGAG TACATCGTGG AGTACTCGCT GGAGTATGGC 
TTCCTGAGGT TGTCGCAGGC CACACGCCAG CGGCTCAGCA TCCCCGTAAT GGTGGTCACG 
CTGGATCCCA CGCGGGACCA GTGCTTTGGT GACCGCTTCA GCCGCCTGCT GCTGGCTGAG 
TTCCTGGGCT ATGATGACAT CCTCATGTCC AGCGTGAAGG GCCTGGCGGA GAACGAGGAG 
AACAAAGGCT TCCTGAGGAA TGTGGTGTCC GGGGAGCACT ACCGCTTCGT GAGCATGTGG 
ATGGCCCGCA CGTCCTACCT GGCCGCCTTC GTCATCATGG TCATCTTTAC CCTGAGCGTG 
TCCATGTTGC TGCGCTACTC CCACCATCAG ATCTTCGTTT TCATTGTGGA CCTGCTGCAG 
ATGCTGGAGA TGAACATGGC CATCGCCTTC CCCGCAGCGC CCCTGCTGAC CGTCATCCTG 
GCCCTCGTGG GAATGGAGGC GATCATGTCT GAGTTCTTCA ACGACACCAC CACGGCCTTC 
TACATCATCC TCATCGTGTG GCTGGCTGAC CAGTATGACG CCATCTGCTG CCACACCAAC 
ACCAGCAAGC GGCACTGGCT TCGGTGGGCA CCAGTGGCCA TGTGGTCCGG GGTGGAGGCA 
GCCGGGTGGG CAGCACCTCA TTGTTCCCCT TGCAGGTTCT TCTACCTGTA CCACTTTGCC 
TTCTACGCTT ACCACTATCG CTTCAACGGG CAGTACAGCA GCCTGGCACT CGTCACCTCC 
TGGCTCTTCA TCCAGCATTC CATGATCTAC TTCTTCCACC ACTACGAACT ACCTGCCATC 
CTGCAGCAGA TCCGTGTCCA GGAGATGCTG CTGCAGACAC CACCACTGGG CCCCGGCACC 
CCCACAGCCC TGCCAGACGA CCTGAACAAC AATGGAGGCA TCCCACCTAT CACCCCCGAC 
GCTGCCAGCC AGTCCCCTGC CCTGGGCCCT GGCTTGCCAG GTGCTGGCGG GGGCCCCGGG 
CCCATGGCTG AGGCACCTAG CTCCCTGGTG GCCGCAGCGG CCTCAGTGGC TGCAGCAGCC 
AGTGGTGACC TGGGTTGGAT GGCAGAGACG GCTGCCATCA TCACAGACGC CTCCTTCCTG 
TCTGGCCTGA GCGCCTCTCT CCTGGACCGG CGGCCAGGCA GCCCCCTGAG CCCTAGTGGG 
GGGCTCCTAC GGGCCCCCCA GGACAGTGCC CCTGCAAGCA ACTCTCCAGG GTCTGATGCA 
GTCTCTCAGA CCACCGGCGG GGGCAGGCCC AACCCTGTGT CTACCGATCT AGTGGACACA 
CCGTCGGAGG TCGGCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319734.1
CDS	96..1916
Translation	MAGPVCVLSWGAPWVGAGATVADLRGNLNSLSTEKGLRQALFVLFVLAYIHIVFSRSPINCLEHVRDKWPREGILRVEVQHNSSRAPVFLQFCDGGHGSFPGLAMEPDGLELEEEEEEELTMDMFGNSSIKFELDIEPKVFKPPGGPKALNDSQEFPFPETPPKAWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLAEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIVDLLQMLEMNMAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRWAPVAMWSGVEAAGWAAPHCSPCRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRVQEMLLQTPPLGPGTPTALPDDLNNNGGIPPITPDAASQSPALGPGLPGAGGGPGPMAEAPSSLVAAAASVAAAASGDLGWMAETAAIITDASFLSGLSASLLDRRPGSPLSPSGGLLRAPQDSAPASNSPGSDAVSQTTGGGRPNPVSTDLVDTPSEVGS

Target ORF information:

RefSeq Version	XM_015464248.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464248.1

ORF Insert Sequence:

ATGGCTGGTC CTGTTTGTGT CCTGTCCTGG GGGGCCCCCT GGGTGGGGGC CGGGGCTACG 
GTTGCCGACT TACGAGGGAA CTTGAATTCC TTGTCCACAG AAAAGGGTCT GAGGCAGGCC 
CTGTTCGTGC TCTTTGTCCT GGCCTACATC CACATCGTCT TCTCCCGCTC ACCCATCAAC 
TGCCTGGAGC ACGTGCGTGA CAAGTGGCCC CGTGAGGGCA TCCTGCGGGT AGAGGTGCAG 
CACAACTCGA GCCGCGCGCC CGTCTTCCTG CAGTTCTGTG ATGGTGGCCA TGGCAGCTTC 
CCTGGCCTGG CCATGGAGCC AGATGGCCTG GAGCTAGAGG AGGAGGAGGA GGAGGAGCTG 
ACCATGGACA TGTTCGGGAA TAGCTCCATC AAGTTTGAAC TGGACATCGA GCCCAAGGTG 
TTCAAGCCAC CAGGTGGCCC CAAGGCCTTA AATGACAGCC AGGAGTTCCC ATTCCCTGAG 
ACGCCCCCAA AAGCCTGGCC ACAAGACGAG TACATCGTGG AGTACTCGCT GGAGTATGGC 
TTCCTGAGGT TGTCGCAGGC CACACGCCAG CGGCTCAGCA TCCCCGTAAT GGTGGTCACG 
CTGGATCCCA CGCGGGACCA GTGCTTTGGT GACCGCTTCA GCCGCCTGCT GCTGGCTGAG 
TTCCTGGGCT ATGATGACAT CCTCATGTCC AGCGTGAAGG GCCTGGCGGA GAACGAGGAG 
AACAAAGGCT TCCTGAGGAA TGTGGTGTCC GGGGAGCACT ACCGCTTCGT GAGCATGTGG 
ATGGCCCGCA CGTCCTACCT GGCCGCCTTC GTCATCATGG TCATCTTTAC CCTGAGCGTG 
TCCATGTTGC TGCGCTACTC CCACCATCAG ATCTTCGTTT TCATTGTGGA CCTGCTGCAG 
ATGCTGGAGA TGAACATGGC CATCGCCTTC CCCGCAGCGC CCCTGCTGAC CGTCATCCTG 
GCCCTCGTGG GAATGGAGGC GATCATGTCT GAGTTCTTCA ACGACACCAC CACGGCCTTC 
TACATCATCC TCATCGTGTG GCTGGCTGAC CAGTATGACG CCATCTGCTG CCACACCAAC 
ACCAGCAAGC GGCACTGGCT TCGGTGGGCA CCAGTGGCCA TGTGGTCCGG GGTGGAGGCA 
GCCGGGTGGG CAGCACCTCA TTGTTCCCCT TGCAGGTTCT TCTACCTGTA CCACTTTGCC 
TTCTACGCTT ACCACTATCG CTTCAACGGG CAGTACAGCA GCCTGGCACT CGTCACCTCC 
TGGCTCTTCA TCCAGCATTC CATGATCTAC TTCTTCCACC ACTACGAACT ACCTGCCATC 
CTGCAGCAGA TCCGTGTCCA GGAGATGCTG CTGCAGACAC CACCACTGGG CCCCGGCACC 
CCCACAGCCC TGCCAGACGA CCTGAACAAC AATGGAGGCA TCCCACCTAT CACCCCCGAC 
GCTGCCAGCC AGTCCCCTGC CCTGGGCCCT GGCTTGCCAG GTGCTGGCGG GGGCCCCGGG 
CCCATGGCTG AGGCACCTAG CTCCCTGGTG GCCGCAGCGG CCTCAGTGGC TGCAGCAGCC 
AGTGGTGACC TGGGTTGGAT GGCAGAGACG GCTGCCATCA TCACAGACGC CTCCTTCCTG 
TCTGGCCTGA GCGCCTCTCT CCTGGACCGG CGGCCAGGCA GCCCCCTGAG CCCTAGTGGG 
GGGCTCCTAC GGGCCCCCCA GGACAGTGCC CCTGCAAGCA ACTCTCCAGG GTCTGATGCA 
GTCTCTCAGA CCACCGGCGG GGGCAGGCCC AACCCTGTGT CTACCGATCT AGTGGACACA 
CCGTCGGAGG TCGGCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM259 ( XM_010807273.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805575.1 Bos taurus transmembrane protein 259 (TMEM259), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa48953
Clone ID Related Accession (Same CDS sequence)	XM_010807273.2 , XM_015464249.1
Accession Version	XM_010807273.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	membralin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTC CTGTTTGTGT CCTGTCCTGG GGGGCCCCCT GGGTGGGGGC CGGGGCTACG 
GTTGCCGACT TACGAGGGAA CTTGAATTCC TTGTCCACAG AAAAGGGTCT GAGGCAGGCC 
CTGTTCGTGC TCTTTGTCCT GGCCTACATC CACATCGTCT TCTCCCGCTC ACCCATCAAC 
TGCCTGGAGC ACGTGCGTGA CAAGTGGCCC CGTGAGGGCA TCCTGCGGGT AGAGGTGCAG 
CACAACTCGA GCCGCGCGCC CGTCTTCCTG CAGTTCTGTG ATGGTGGCCA TGGCAGCTTC 
CCTGGCCTGG CCATGGAGCC AGATGGCCTG GAGCTAGAGG AGGAGGAGGA GGAGGAGCTG 
ACCATGGACA TGTTCGGGAA TAGCTCCATC AAGTTTGAAC TGGACATCGA GCCCAAGGTG 
TTCAAGCCAC CAGGTGGCCC CAAGGCCTTA AATGACAGCC AGGAGTTCCC ATTCCCTGAG 
ACGCCCCCAA AAGCCTGGCC ACAAGACGAG TACATCGTGG AGTACTCGCT GGAGTATGGC 
TTCCTGAGGT TGTCGCAGGC CACACGCCAG CGGCTCAGCA TCCCCGTAAT GGTGGTCACG 
CTGGATCCCA CGCGGGACCA GTGCTTTGGT GACCGCTTCA GCCGCCTGCT GCTGGCTGAG 
TTCCTGGGCT ATGATGACAT CCTCATGTCC AGCGTGAAGG GCCTGGCGGA GAACGAGGAG 
AACAAAGGCT TCCTGAGGAA TGTGGTGTCC GGGGAGCACT ACCGCTTCGT GAGCATGTGG 
ATGGCCCGCA CGTCCTACCT GGCCGCCTTC GTCATCATGG TCATCTTTAC CCTGAGCGTG 
TCCATGTTGC TGCGCTACTC CCACCATCAG ATCTTCGTTT TCATTGTGGA CCTGCTGCAG 
ATGCTGGAGA TGAACATGGC CATCGCCTTC CCCGCAGCGC CCCTGCTGAC CGTCATCCTG 
GCCCTCGTGG GAATGGAGGC GATCATGTCT GAGTTCTTCA ACGACACCAC CACGGCCTTC 
TACATCATCC TCATCGTGTG GCTGGCTGAC CAGTATGACG CCATCTGCTG CCACACCAAC 
ACCAGCAAGC GGCACTGGCT TCGGTTCTTC TACCTGTACC ACTTTGCCTT CTACGCTTAC 
CACTATCGCT TCAACGGGCA GTACAGCAGC CTGGCACTCG TCACCTCCTG GCTCTTCATC 
CAGCATTCCA TGATCTACTT CTTCCACCAC TACGAACTAC CTGCCATCCT GCAGCAGATC 
CGTGTCCAGG AGATGCTGCT GCAGACACCA CCACTGGGCC CCGGCACCCC CACAGCCCTG 
CCAGACGACC TGAACAACAA TGGAGGCATC CCACCTATCA CCCCCGACGC TGCCAGCCAG 
TCCCCTGCCC TGGGCCCTGG CTTGCCAGGT GCTGGCGGGG GCCCCGGGCC CATGGCTGAG 
GCACCTAGCT CCCTGGTGGC CGCAGCGGCC TCAGTGGCTG CAGCAGCCAG TGGTGACCTG 
GGTTGGATGG CAGAGACGGC TGCCATCATC ACAGACGCCT CCTTCCTGTC TGGCCTGAGC 
GCCTCTCTCC TGGACCGGCG GCCAGGCAGC CCCCTGAGCC CTAGTGGGGG GCTCCTACGG 
GCCCCCCAGG ACAGTGCCCC TGCAAGCAAC TCTCCAGGGT CTGATGCAGT CTCTCAGACC 
ACCGGCGGGG GCAGGCCCAA CCCTGTGTCT ACCGATCTAG TGGACACACC GTCGGAGGTC 
GGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805575.1
CDS	89..1837
Translation	MAGPVCVLSWGAPWVGAGATVADLRGNLNSLSTEKGLRQALFVLFVLAYIHIVFSRSPINCLEHVRDKWPREGILRVEVQHNSSRAPVFLQFCDGGHGSFPGLAMEPDGLELEEEEEEELTMDMFGNSSIKFELDIEPKVFKPPGGPKALNDSQEFPFPETPPKAWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLAEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIVDLLQMLEMNMAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRVQEMLLQTPPLGPGTPTALPDDLNNNGGIPPITPDAASQSPALGPGLPGAGGGPGPMAEAPSSLVAAAASVAAAASGDLGWMAETAAIITDASFLSGLSASLLDRRPGSPLSPSGGLLRAPQDSAPASNSPGSDAVSQTTGGGRPNPVSTDLVDTPSEVGS

Target ORF information:

RefSeq Version	XM_010807273.2
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807273.2

ORF Insert Sequence:

ATGGCTGGTC CTGTTTGTGT CCTGTCCTGG GGGGCCCCCT GGGTGGGGGC CGGGGCTACG 
GTTGCCGACT TACGAGGGAA CTTGAATTCC TTGTCCACAG AAAAGGGTCT GAGGCAGGCC 
CTGTTCGTGC TCTTTGTCCT GGCCTACATC CACATCGTCT TCTCCCGCTC ACCCATCAAC 
TGCCTGGAGC ACGTGCGTGA CAAGTGGCCC CGTGAGGGCA TCCTGCGGGT AGAGGTGCAG 
CACAACTCGA GCCGCGCGCC CGTCTTCCTG CAGTTCTGTG ATGGTGGCCA TGGCAGCTTC 
CCTGGCCTGG CCATGGAGCC AGATGGCCTG GAGCTAGAGG AGGAGGAGGA GGAGGAGCTG 
ACCATGGACA TGTTCGGGAA TAGCTCCATC AAGTTTGAAC TGGACATCGA GCCCAAGGTG 
TTCAAGCCAC CAGGTGGCCC CAAGGCCTTA AATGACAGCC AGGAGTTCCC ATTCCCTGAG 
ACGCCCCCAA AAGCCTGGCC ACAAGACGAG TACATCGTGG AGTACTCGCT GGAGTATGGC 
TTCCTGAGGT TGTCGCAGGC CACACGCCAG CGGCTCAGCA TCCCCGTAAT GGTGGTCACG 
CTGGATCCCA CGCGGGACCA GTGCTTTGGT GACCGCTTCA GCCGCCTGCT GCTGGCTGAG 
TTCCTGGGCT ATGATGACAT CCTCATGTCC AGCGTGAAGG GCCTGGCGGA GAACGAGGAG 
AACAAAGGCT TCCTGAGGAA TGTGGTGTCC GGGGAGCACT ACCGCTTCGT GAGCATGTGG 
ATGGCCCGCA CGTCCTACCT GGCCGCCTTC GTCATCATGG TCATCTTTAC CCTGAGCGTG 
TCCATGTTGC TGCGCTACTC CCACCATCAG ATCTTCGTTT TCATTGTGGA CCTGCTGCAG 
ATGCTGGAGA TGAACATGGC CATCGCCTTC CCCGCAGCGC CCCTGCTGAC CGTCATCCTG 
GCCCTCGTGG GAATGGAGGC GATCATGTCT GAGTTCTTCA ACGACACCAC CACGGCCTTC 
TACATCATCC TCATCGTGTG GCTGGCTGAC CAGTATGACG CCATCTGCTG CCACACCAAC 
ACCAGCAAGC GGCACTGGCT TCGGTTCTTC TACCTGTACC ACTTTGCCTT CTACGCTTAC 
CACTATCGCT TCAACGGGCA GTACAGCAGC CTGGCACTCG TCACCTCCTG GCTCTTCATC 
CAGCATTCCA TGATCTACTT CTTCCACCAC TACGAACTAC CTGCCATCCT GCAGCAGATC 
CGTGTCCAGG AGATGCTGCT GCAGACACCA CCACTGGGCC CCGGCACCCC CACAGCCCTG 
CCAGACGACC TGAACAACAA TGGAGGCATC CCACCTATCA CCCCCGACGC TGCCAGCCAG 
TCCCCTGCCC TGGGCCCTGG CTTGCCAGGT GCTGGCGGGG GCCCCGGGCC CATGGCTGAG 
GCACCTAGCT CCCTGGTGGC CGCAGCGGCC TCAGTGGCTG CAGCAGCCAG TGGTGACCTG 
GGTTGGATGG CAGAGACGGC TGCCATCATC ACAGACGCCT CCTTCCTGTC TGGCCTGAGC 
GCCTCTCTCC TGGACCGGCG GCCAGGCAGC CCCCTGAGCC CTAGTGGGGG GCTCCTACGG 
GCCCCCCAGG ACAGTGCCCC TGCAAGCAAC TCTCCAGGGT CTGATGCAGT CTCTCAGACC 
ACCGGCGGGG GCAGGCCCAA CCCTGTGTCT ACCGATCTAG TGGACACACC GTCGGAGGTC 
GGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM259 ( XM_015464249.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319735.1 Bos taurus transmembrane protein 259 (TMEM259), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa48953
Clone ID Related Accession (Same CDS sequence)	XM_010807273.2 , XM_015464249.1
Accession Version	XM_015464249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	membralin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTC CTGTTTGTGT CCTGTCCTGG GGGGCCCCCT GGGTGGGGGC CGGGGCTACG 
GTTGCCGACT TACGAGGGAA CTTGAATTCC TTGTCCACAG AAAAGGGTCT GAGGCAGGCC 
CTGTTCGTGC TCTTTGTCCT GGCCTACATC CACATCGTCT TCTCCCGCTC ACCCATCAAC 
TGCCTGGAGC ACGTGCGTGA CAAGTGGCCC CGTGAGGGCA TCCTGCGGGT AGAGGTGCAG 
CACAACTCGA GCCGCGCGCC CGTCTTCCTG CAGTTCTGTG ATGGTGGCCA TGGCAGCTTC 
CCTGGCCTGG CCATGGAGCC AGATGGCCTG GAGCTAGAGG AGGAGGAGGA GGAGGAGCTG 
ACCATGGACA TGTTCGGGAA TAGCTCCATC AAGTTTGAAC TGGACATCGA GCCCAAGGTG 
TTCAAGCCAC CAGGTGGCCC CAAGGCCTTA AATGACAGCC AGGAGTTCCC ATTCCCTGAG 
ACGCCCCCAA AAGCCTGGCC ACAAGACGAG TACATCGTGG AGTACTCGCT GGAGTATGGC 
TTCCTGAGGT TGTCGCAGGC CACACGCCAG CGGCTCAGCA TCCCCGTAAT GGTGGTCACG 
CTGGATCCCA CGCGGGACCA GTGCTTTGGT GACCGCTTCA GCCGCCTGCT GCTGGCTGAG 
TTCCTGGGCT ATGATGACAT CCTCATGTCC AGCGTGAAGG GCCTGGCGGA GAACGAGGAG 
AACAAAGGCT TCCTGAGGAA TGTGGTGTCC GGGGAGCACT ACCGCTTCGT GAGCATGTGG 
ATGGCCCGCA CGTCCTACCT GGCCGCCTTC GTCATCATGG TCATCTTTAC CCTGAGCGTG 
TCCATGTTGC TGCGCTACTC CCACCATCAG ATCTTCGTTT TCATTGTGGA CCTGCTGCAG 
ATGCTGGAGA TGAACATGGC CATCGCCTTC CCCGCAGCGC CCCTGCTGAC CGTCATCCTG 
GCCCTCGTGG GAATGGAGGC GATCATGTCT GAGTTCTTCA ACGACACCAC CACGGCCTTC 
TACATCATCC TCATCGTGTG GCTGGCTGAC CAGTATGACG CCATCTGCTG CCACACCAAC 
ACCAGCAAGC GGCACTGGCT TCGGTTCTTC TACCTGTACC ACTTTGCCTT CTACGCTTAC 
CACTATCGCT TCAACGGGCA GTACAGCAGC CTGGCACTCG TCACCTCCTG GCTCTTCATC 
CAGCATTCCA TGATCTACTT CTTCCACCAC TACGAACTAC CTGCCATCCT GCAGCAGATC 
CGTGTCCAGG AGATGCTGCT GCAGACACCA CCACTGGGCC CCGGCACCCC CACAGCCCTG 
CCAGACGACC TGAACAACAA TGGAGGCATC CCACCTATCA CCCCCGACGC TGCCAGCCAG 
TCCCCTGCCC TGGGCCCTGG CTTGCCAGGT GCTGGCGGGG GCCCCGGGCC CATGGCTGAG 
GCACCTAGCT CCCTGGTGGC CGCAGCGGCC TCAGTGGCTG CAGCAGCCAG TGGTGACCTG 
GGTTGGATGG CAGAGACGGC TGCCATCATC ACAGACGCCT CCTTCCTGTC TGGCCTGAGC 
GCCTCTCTCC TGGACCGGCG GCCAGGCAGC CCCCTGAGCC CTAGTGGGGG GCTCCTACGG 
GCCCCCCAGG ACAGTGCCCC TGCAAGCAAC TCTCCAGGGT CTGATGCAGT CTCTCAGACC 
ACCGGCGGGG GCAGGCCCAA CCCTGTGTCT ACCGATCTAG TGGACACACC GTCGGAGGTC 
GGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319735.1
CDS	89..1837
Translation	MAGPVCVLSWGAPWVGAGATVADLRGNLNSLSTEKGLRQALFVLFVLAYIHIVFSRSPINCLEHVRDKWPREGILRVEVQHNSSRAPVFLQFCDGGHGSFPGLAMEPDGLELEEEEEEELTMDMFGNSSIKFELDIEPKVFKPPGGPKALNDSQEFPFPETPPKAWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLAEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIVDLLQMLEMNMAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRVQEMLLQTPPLGPGTPTALPDDLNNNGGIPPITPDAASQSPALGPGLPGAGGGPGPMAEAPSSLVAAAASVAAAASGDLGWMAETAAIITDASFLSGLSASLLDRRPGSPLSPSGGLLRAPQDSAPASNSPGSDAVSQTTGGGRPNPVSTDLVDTPSEVGS

Target ORF information:

RefSeq Version	XM_015464249.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464249.1

ORF Insert Sequence:

ATGGCTGGTC CTGTTTGTGT CCTGTCCTGG GGGGCCCCCT GGGTGGGGGC CGGGGCTACG 
GTTGCCGACT TACGAGGGAA CTTGAATTCC TTGTCCACAG AAAAGGGTCT GAGGCAGGCC 
CTGTTCGTGC TCTTTGTCCT GGCCTACATC CACATCGTCT TCTCCCGCTC ACCCATCAAC 
TGCCTGGAGC ACGTGCGTGA CAAGTGGCCC CGTGAGGGCA TCCTGCGGGT AGAGGTGCAG 
CACAACTCGA GCCGCGCGCC CGTCTTCCTG CAGTTCTGTG ATGGTGGCCA TGGCAGCTTC 
CCTGGCCTGG CCATGGAGCC AGATGGCCTG GAGCTAGAGG AGGAGGAGGA GGAGGAGCTG 
ACCATGGACA TGTTCGGGAA TAGCTCCATC AAGTTTGAAC TGGACATCGA GCCCAAGGTG 
TTCAAGCCAC CAGGTGGCCC CAAGGCCTTA AATGACAGCC AGGAGTTCCC ATTCCCTGAG 
ACGCCCCCAA AAGCCTGGCC ACAAGACGAG TACATCGTGG AGTACTCGCT GGAGTATGGC 
TTCCTGAGGT TGTCGCAGGC CACACGCCAG CGGCTCAGCA TCCCCGTAAT GGTGGTCACG 
CTGGATCCCA CGCGGGACCA GTGCTTTGGT GACCGCTTCA GCCGCCTGCT GCTGGCTGAG 
TTCCTGGGCT ATGATGACAT CCTCATGTCC AGCGTGAAGG GCCTGGCGGA GAACGAGGAG 
AACAAAGGCT TCCTGAGGAA TGTGGTGTCC GGGGAGCACT ACCGCTTCGT GAGCATGTGG 
ATGGCCCGCA CGTCCTACCT GGCCGCCTTC GTCATCATGG TCATCTTTAC CCTGAGCGTG 
TCCATGTTGC TGCGCTACTC CCACCATCAG ATCTTCGTTT TCATTGTGGA CCTGCTGCAG 
ATGCTGGAGA TGAACATGGC CATCGCCTTC CCCGCAGCGC CCCTGCTGAC CGTCATCCTG 
GCCCTCGTGG GAATGGAGGC GATCATGTCT GAGTTCTTCA ACGACACCAC CACGGCCTTC 
TACATCATCC TCATCGTGTG GCTGGCTGAC CAGTATGACG CCATCTGCTG CCACACCAAC 
ACCAGCAAGC GGCACTGGCT TCGGTTCTTC TACCTGTACC ACTTTGCCTT CTACGCTTAC 
CACTATCGCT TCAACGGGCA GTACAGCAGC CTGGCACTCG TCACCTCCTG GCTCTTCATC 
CAGCATTCCA TGATCTACTT CTTCCACCAC TACGAACTAC CTGCCATCCT GCAGCAGATC 
CGTGTCCAGG AGATGCTGCT GCAGACACCA CCACTGGGCC CCGGCACCCC CACAGCCCTG 
CCAGACGACC TGAACAACAA TGGAGGCATC CCACCTATCA CCCCCGACGC TGCCAGCCAG 
TCCCCTGCCC TGGGCCCTGG CTTGCCAGGT GCTGGCGGGG GCCCCGGGCC CATGGCTGAG 
GCACCTAGCT CCCTGGTGGC CGCAGCGGCC TCAGTGGCTG CAGCAGCCAG TGGTGACCTG 
GGTTGGATGG CAGAGACGGC TGCCATCATC ACAGACGCCT CCTTCCTGTC TGGCCTGAGC 
GCCTCTCTCC TGGACCGGCG GCCAGGCAGC CCCCTGAGCC CTAGTGGGGG GCTCCTACGG 
GCCCCCCAGG ACAGTGCCCC TGCAAGCAAC TCTCCAGGGT CTGATGCAGT CTCTCAGACC 
ACCGGCGGGG GCAGGCCCAA CCCTGTGTCT ACCGATCTAG TGGACACACC GTCGGAGGTC 
GGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM259 ( XM_010807274.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805576.1 Bos taurus transmembrane protein 259 (TMEM259), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa48954
Clone ID Related Accession (Same CDS sequence)	XM_010807274.2 , XM_015464250.1
Accession Version	XM_010807274.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	membralin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807274.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTC CTGTTTGTGT CCTGTCCTGG GGGGCCCCCT GGGTGGGGGC CGGGGCTACG 
GTTGCCGACT TACGAGGGAA CTTGAATTCC TTGTCCACAG AAAAGGGTCT GAGGCAGGCC 
CTGTTCGTGC TCTTTGTCCT GGCCTACATC CACATCGTCT TCTCCCGCTC ACCCATCAAC 
TGCCTGGAGC ACGTGCGTGA CAAGTGGCCC CGTGAGGGCA TCCTGCGGGT AGAGGTGCAG 
CACAACTCGA GCCGCGCGCC CGTCTTCCTG CAGTTCTGTG ATGGTGGCCA TGGCAGCTTC 
CCTGGCCTGG CCATGGAGCC AGATGGCCTG GAGCTAGAGG AGGAGGAGGA GGAGGAGCTG 
ACCATGGACA TGTTCGGGAA TAGCTCCATC AAGTTTGAAC TGGACATCGA GCCCAAGGTG 
TTCAAGCCAC CAGGTGGCCC CAAGGCCTTA AATGACAGCC AGGAGTTCCC ATTCCCTGAG 
ACGCCCCCAA AAGCCTGGCC ACAAGACGAG TACATCGTGG AGTACTCGCT GGAGTATGGC 
TTCCTGAGGT TGTCGCAGGC CACACGCCAG CGGCTCAGCA TCCCCGTAAT GGTGGTCACG 
CTGGATCCCA CGCGGGACCA GTGCTTTGGT GACCGCTTCA GCCGCCTGCT GCTGGCTGAG 
TTCCTGGGCT ATGATGACAT CCTCATGTCC AGCGTGAAGG GCCTGGCGGA GAACGAGGAG 
AACAAAGGCT TCCTGAGGAA TGTGGTGTCC GGGGAGCACT ACCGCTTCGT GAGCATGTGG 
ATGGCCCGCA CGTCCTACCT GGCCGCCTTC GTCATCATGG TCATCTTTAC CCTGAGCGTG 
TCCATGTTGC TGCGCTACTC CCACCATCAG ATCTTCGTTT TCATTGACCT GCTGCAGATG 
CTGGAGATGA ACATGGCCAT CGCCTTCCCC GCAGCGCCCC TGCTGACCGT CATCCTGGCC 
CTCGTGGGAA TGGAGGCGAT CATGTCTGAG TTCTTCAACG ACACCACCAC GGCCTTCTAC 
ATCATCCTCA TCGTGTGGCT GGCTGACCAG TATGACGCCA TCTGCTGCCA CACCAACACC 
AGCAAGCGGC ACTGGCTTCG GTTCTTCTAC CTGTACCACT TTGCCTTCTA CGCTTACCAC 
TATCGCTTCA ACGGGCAGTA CAGCAGCCTG GCACTCGTCA CCTCCTGGCT CTTCATCCAG 
CATTCCATGA TCTACTTCTT CCACCACTAC GAACTACCTG CCATCCTGCA GCAGATCCGT 
GTCCAGGAGA TGCTGCTGCA GACACCACCA CTGGGCCCCG GCACCCCCAC AGCCCTGCCA 
GACGACCTGA ACAACAATGG AGGCATCCCA CCTATCACCC CCGACGCTGC CAGCCAGTCC 
CCTGCCCTGG GCCCTGGCTT GCCAGGTGCT GGCGGGGGCC CCGGGCCCAT GGCTGAGGCA 
CCTAGCTCCC TGGTGGCCGC AGCGGCCTCA GTGGCTGCAG CAGCCAGTGG TGACCTGGGT 
TGGATGGCAG AGACGGCTGC CATCATCACA GACGCCTCCT TCCTGTCTGG CCTGAGCGCC 
TCTCTCCTGG ACCGGCGGCC AGGCAGCCCC CTGAGCCCTA GTGGGGGGCT CCTACGGGCC 
CCCCAGGACA GTGCCCCTGC AAGCAACTCT CCAGGGTCTG ATGCAGTCTC TCAGACCACC 
GGCGGGGGCA GGCCCAACCC TGTGTCTACC GATCTAGTGG ACACACCGTC GGAGGTCGGC 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805576.1
CDS	90..1835
Translation	MAGPVCVLSWGAPWVGAGATVADLRGNLNSLSTEKGLRQALFVLFVLAYIHIVFSRSPINCLEHVRDKWPREGILRVEVQHNSSRAPVFLQFCDGGHGSFPGLAMEPDGLELEEEEEEELTMDMFGNSSIKFELDIEPKVFKPPGGPKALNDSQEFPFPETPPKAWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLAEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIDLLQMLEMNMAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRVQEMLLQTPPLGPGTPTALPDDLNNNGGIPPITPDAASQSPALGPGLPGAGGGPGPMAEAPSSLVAAAASVAAAASGDLGWMAETAAIITDASFLSGLSASLLDRRPGSPLSPSGGLLRAPQDSAPASNSPGSDAVSQTTGGGRPNPVSTDLVDTPSEVGS

Target ORF information:

RefSeq Version	XM_010807274.2
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807274.2

ORF Insert Sequence:

ATGGCTGGTC CTGTTTGTGT CCTGTCCTGG GGGGCCCCCT GGGTGGGGGC CGGGGCTACG 
GTTGCCGACT TACGAGGGAA CTTGAATTCC TTGTCCACAG AAAAGGGTCT GAGGCAGGCC 
CTGTTCGTGC TCTTTGTCCT GGCCTACATC CACATCGTCT TCTCCCGCTC ACCCATCAAC 
TGCCTGGAGC ACGTGCGTGA CAAGTGGCCC CGTGAGGGCA TCCTGCGGGT AGAGGTGCAG 
CACAACTCGA GCCGCGCGCC CGTCTTCCTG CAGTTCTGTG ATGGTGGCCA TGGCAGCTTC 
CCTGGCCTGG CCATGGAGCC AGATGGCCTG GAGCTAGAGG AGGAGGAGGA GGAGGAGCTG 
ACCATGGACA TGTTCGGGAA TAGCTCCATC AAGTTTGAAC TGGACATCGA GCCCAAGGTG 
TTCAAGCCAC CAGGTGGCCC CAAGGCCTTA AATGACAGCC AGGAGTTCCC ATTCCCTGAG 
ACGCCCCCAA AAGCCTGGCC ACAAGACGAG TACATCGTGG AGTACTCGCT GGAGTATGGC 
TTCCTGAGGT TGTCGCAGGC CACACGCCAG CGGCTCAGCA TCCCCGTAAT GGTGGTCACG 
CTGGATCCCA CGCGGGACCA GTGCTTTGGT GACCGCTTCA GCCGCCTGCT GCTGGCTGAG 
TTCCTGGGCT ATGATGACAT CCTCATGTCC AGCGTGAAGG GCCTGGCGGA GAACGAGGAG 
AACAAAGGCT TCCTGAGGAA TGTGGTGTCC GGGGAGCACT ACCGCTTCGT GAGCATGTGG 
ATGGCCCGCA CGTCCTACCT GGCCGCCTTC GTCATCATGG TCATCTTTAC CCTGAGCGTG 
TCCATGTTGC TGCGCTACTC CCACCATCAG ATCTTCGTTT TCATTGACCT GCTGCAGATG 
CTGGAGATGA ACATGGCCAT CGCCTTCCCC GCAGCGCCCC TGCTGACCGT CATCCTGGCC 
CTCGTGGGAA TGGAGGCGAT CATGTCTGAG TTCTTCAACG ACACCACCAC GGCCTTCTAC 
ATCATCCTCA TCGTGTGGCT GGCTGACCAG TATGACGCCA TCTGCTGCCA CACCAACACC 
AGCAAGCGGC ACTGGCTTCG GTTCTTCTAC CTGTACCACT TTGCCTTCTA CGCTTACCAC 
TATCGCTTCA ACGGGCAGTA CAGCAGCCTG GCACTCGTCA CCTCCTGGCT CTTCATCCAG 
CATTCCATGA TCTACTTCTT CCACCACTAC GAACTACCTG CCATCCTGCA GCAGATCCGT 
GTCCAGGAGA TGCTGCTGCA GACACCACCA CTGGGCCCCG GCACCCCCAC AGCCCTGCCA 
GACGACCTGA ACAACAATGG AGGCATCCCA CCTATCACCC CCGACGCTGC CAGCCAGTCC 
CCTGCCCTGG GCCCTGGCTT GCCAGGTGCT GGCGGGGGCC CCGGGCCCAT GGCTGAGGCA 
CCTAGCTCCC TGGTGGCCGC AGCGGCCTCA GTGGCTGCAG CAGCCAGTGG TGACCTGGGT 
TGGATGGCAG AGACGGCTGC CATCATCACA GACGCCTCCT TCCTGTCTGG CCTGAGCGCC 
TCTCTCCTGG ACCGGCGGCC AGGCAGCCCC CTGAGCCCTA GTGGGGGGCT CCTACGGGCC 
CCCCAGGACA GTGCCCCTGC AAGCAACTCT CCAGGGTCTG ATGCAGTCTC TCAGACCACC 
GGCGGGGGCA GGCCCAACCC TGTGTCTACC GATCTAGTGG ACACACCGTC GGAGGTCGGC 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM259 ( XM_015464250.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319736.1 Bos taurus transmembrane protein 259 (TMEM259), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa48954
Clone ID Related Accession (Same CDS sequence)	XM_010807274.2 , XM_015464250.1
Accession Version	XM_015464250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	membralin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTC CTGTTTGTGT CCTGTCCTGG GGGGCCCCCT GGGTGGGGGC CGGGGCTACG 
GTTGCCGACT TACGAGGGAA CTTGAATTCC TTGTCCACAG AAAAGGGTCT GAGGCAGGCC 
CTGTTCGTGC TCTTTGTCCT GGCCTACATC CACATCGTCT TCTCCCGCTC ACCCATCAAC 
TGCCTGGAGC ACGTGCGTGA CAAGTGGCCC CGTGAGGGCA TCCTGCGGGT AGAGGTGCAG 
CACAACTCGA GCCGCGCGCC CGTCTTCCTG CAGTTCTGTG ATGGTGGCCA TGGCAGCTTC 
CCTGGCCTGG CCATGGAGCC AGATGGCCTG GAGCTAGAGG AGGAGGAGGA GGAGGAGCTG 
ACCATGGACA TGTTCGGGAA TAGCTCCATC AAGTTTGAAC TGGACATCGA GCCCAAGGTG 
TTCAAGCCAC CAGGTGGCCC CAAGGCCTTA AATGACAGCC AGGAGTTCCC ATTCCCTGAG 
ACGCCCCCAA AAGCCTGGCC ACAAGACGAG TACATCGTGG AGTACTCGCT GGAGTATGGC 
TTCCTGAGGT TGTCGCAGGC CACACGCCAG CGGCTCAGCA TCCCCGTAAT GGTGGTCACG 
CTGGATCCCA CGCGGGACCA GTGCTTTGGT GACCGCTTCA GCCGCCTGCT GCTGGCTGAG 
TTCCTGGGCT ATGATGACAT CCTCATGTCC AGCGTGAAGG GCCTGGCGGA GAACGAGGAG 
AACAAAGGCT TCCTGAGGAA TGTGGTGTCC GGGGAGCACT ACCGCTTCGT GAGCATGTGG 
ATGGCCCGCA CGTCCTACCT GGCCGCCTTC GTCATCATGG TCATCTTTAC CCTGAGCGTG 
TCCATGTTGC TGCGCTACTC CCACCATCAG ATCTTCGTTT TCATTGACCT GCTGCAGATG 
CTGGAGATGA ACATGGCCAT CGCCTTCCCC GCAGCGCCCC TGCTGACCGT CATCCTGGCC 
CTCGTGGGAA TGGAGGCGAT CATGTCTGAG TTCTTCAACG ACACCACCAC GGCCTTCTAC 
ATCATCCTCA TCGTGTGGCT GGCTGACCAG TATGACGCCA TCTGCTGCCA CACCAACACC 
AGCAAGCGGC ACTGGCTTCG GTTCTTCTAC CTGTACCACT TTGCCTTCTA CGCTTACCAC 
TATCGCTTCA ACGGGCAGTA CAGCAGCCTG GCACTCGTCA CCTCCTGGCT CTTCATCCAG 
CATTCCATGA TCTACTTCTT CCACCACTAC GAACTACCTG CCATCCTGCA GCAGATCCGT 
GTCCAGGAGA TGCTGCTGCA GACACCACCA CTGGGCCCCG GCACCCCCAC AGCCCTGCCA 
GACGACCTGA ACAACAATGG AGGCATCCCA CCTATCACCC CCGACGCTGC CAGCCAGTCC 
CCTGCCCTGG GCCCTGGCTT GCCAGGTGCT GGCGGGGGCC CCGGGCCCAT GGCTGAGGCA 
CCTAGCTCCC TGGTGGCCGC AGCGGCCTCA GTGGCTGCAG CAGCCAGTGG TGACCTGGGT 
TGGATGGCAG AGACGGCTGC CATCATCACA GACGCCTCCT TCCTGTCTGG CCTGAGCGCC 
TCTCTCCTGG ACCGGCGGCC AGGCAGCCCC CTGAGCCCTA GTGGGGGGCT CCTACGGGCC 
CCCCAGGACA GTGCCCCTGC AAGCAACTCT CCAGGGTCTG ATGCAGTCTC TCAGACCACC 
GGCGGGGGCA GGCCCAACCC TGTGTCTACC GATCTAGTGG ACACACCGTC GGAGGTCGGC 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319736.1
CDS	90..1835
Translation	MAGPVCVLSWGAPWVGAGATVADLRGNLNSLSTEKGLRQALFVLFVLAYIHIVFSRSPINCLEHVRDKWPREGILRVEVQHNSSRAPVFLQFCDGGHGSFPGLAMEPDGLELEEEEEEELTMDMFGNSSIKFELDIEPKVFKPPGGPKALNDSQEFPFPETPPKAWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLAEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIDLLQMLEMNMAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRVQEMLLQTPPLGPGTPTALPDDLNNNGGIPPITPDAASQSPALGPGLPGAGGGPGPMAEAPSSLVAAAASVAAAASGDLGWMAETAAIITDASFLSGLSASLLDRRPGSPLSPSGGLLRAPQDSAPASNSPGSDAVSQTTGGGRPNPVSTDLVDTPSEVGS

Target ORF information:

RefSeq Version	XM_015464250.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464250.1

ORF Insert Sequence:

ATGGCTGGTC CTGTTTGTGT CCTGTCCTGG GGGGCCCCCT GGGTGGGGGC CGGGGCTACG 
GTTGCCGACT TACGAGGGAA CTTGAATTCC TTGTCCACAG AAAAGGGTCT GAGGCAGGCC 
CTGTTCGTGC TCTTTGTCCT GGCCTACATC CACATCGTCT TCTCCCGCTC ACCCATCAAC 
TGCCTGGAGC ACGTGCGTGA CAAGTGGCCC CGTGAGGGCA TCCTGCGGGT AGAGGTGCAG 
CACAACTCGA GCCGCGCGCC CGTCTTCCTG CAGTTCTGTG ATGGTGGCCA TGGCAGCTTC 
CCTGGCCTGG CCATGGAGCC AGATGGCCTG GAGCTAGAGG AGGAGGAGGA GGAGGAGCTG 
ACCATGGACA TGTTCGGGAA TAGCTCCATC AAGTTTGAAC TGGACATCGA GCCCAAGGTG 
TTCAAGCCAC CAGGTGGCCC CAAGGCCTTA AATGACAGCC AGGAGTTCCC ATTCCCTGAG 
ACGCCCCCAA AAGCCTGGCC ACAAGACGAG TACATCGTGG AGTACTCGCT GGAGTATGGC 
TTCCTGAGGT TGTCGCAGGC CACACGCCAG CGGCTCAGCA TCCCCGTAAT GGTGGTCACG 
CTGGATCCCA CGCGGGACCA GTGCTTTGGT GACCGCTTCA GCCGCCTGCT GCTGGCTGAG 
TTCCTGGGCT ATGATGACAT CCTCATGTCC AGCGTGAAGG GCCTGGCGGA GAACGAGGAG 
AACAAAGGCT TCCTGAGGAA TGTGGTGTCC GGGGAGCACT ACCGCTTCGT GAGCATGTGG 
ATGGCCCGCA CGTCCTACCT GGCCGCCTTC GTCATCATGG TCATCTTTAC CCTGAGCGTG 
TCCATGTTGC TGCGCTACTC CCACCATCAG ATCTTCGTTT TCATTGACCT GCTGCAGATG 
CTGGAGATGA ACATGGCCAT CGCCTTCCCC GCAGCGCCCC TGCTGACCGT CATCCTGGCC 
CTCGTGGGAA TGGAGGCGAT CATGTCTGAG TTCTTCAACG ACACCACCAC GGCCTTCTAC 
ATCATCCTCA TCGTGTGGCT GGCTGACCAG TATGACGCCA TCTGCTGCCA CACCAACACC 
AGCAAGCGGC ACTGGCTTCG GTTCTTCTAC CTGTACCACT TTGCCTTCTA CGCTTACCAC 
TATCGCTTCA ACGGGCAGTA CAGCAGCCTG GCACTCGTCA CCTCCTGGCT CTTCATCCAG 
CATTCCATGA TCTACTTCTT CCACCACTAC GAACTACCTG CCATCCTGCA GCAGATCCGT 
GTCCAGGAGA TGCTGCTGCA GACACCACCA CTGGGCCCCG GCACCCCCAC AGCCCTGCCA 
GACGACCTGA ACAACAATGG AGGCATCCCA CCTATCACCC CCGACGCTGC CAGCCAGTCC 
CCTGCCCTGG GCCCTGGCTT GCCAGGTGCT GGCGGGGGCC CCGGGCCCAT GGCTGAGGCA 
CCTAGCTCCC TGGTGGCCGC AGCGGCCTCA GTGGCTGCAG CAGCCAGTGG TGACCTGGGT 
TGGATGGCAG AGACGGCTGC CATCATCACA GACGCCTCCT TCCTGTCTGG CCTGAGCGCC 
TCTCTCCTGG ACCGGCGGCC AGGCAGCCCC CTGAGCCCTA GTGGGGGGCT CCTACGGGCC 
CCCCAGGACA GTGCCCCTGC AAGCAACTCT CCAGGGTCTG ATGCAGTCTC TCAGACCACC 
GGCGGGGGCA GGCCCAACCC TGTGTCTACC GATCTAGTGG ACACACCGTC GGAGGTCGGC 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM259 ( XM_010807275.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805577.1 Bos taurus transmembrane protein 259 (TMEM259), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa48955
Clone ID Related Accession (Same CDS sequence)	XM_010807275.2 , XM_015464251.1
Accession Version	XM_010807275.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	membralin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807275.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAG ATGGCCTGGA GCTAGAGGAG GAGGAGGAGG AGGAGCTGAC CATGGACATG 
TTCGGGAATA GCTCCATCAA GTTTGAACTG GACATCGAGC CCAAGGTGTT CAAGCCACCA 
GGTGGCCCCA AGGCCTTAAA TGACAGCCAG GAGTTCCCAT TCCCTGAGAC GCCCCCAAAA 
GCCTGGCCAC AAGACGAGTA CATCGTGGAG TACTCGCTGG AGTATGGCTT CCTGAGGTTG 
TCGCAGGCCA CACGCCAGCG GCTCAGCATC CCCGTAATGG TGGTCACGCT GGATCCCACG 
CGGGACCAGT GCTTTGGTGA CCGCTTCAGC CGCCTGCTGC TGGCTGAGTT CCTGGGCTAT 
GATGACATCC TCATGTCCAG CGTGAAGGGC CTGGCGGAGA ACGAGGAGAA CAAAGGCTTC 
CTGAGGAATG TGGTGTCCGG GGAGCACTAC CGCTTCGTGA GCATGTGGAT GGCCCGCACG 
TCCTACCTGG CCGCCTTCGT CATCATGGTC ATCTTTACCC TGAGCGTGTC CATGTTGCTG 
CGCTACTCCC ACCATCAGAT CTTCGTTTTC ATTGGTGAGT GTCCCCTCTG GCCATCTCCC 
TTGCCCCTCC CCCATTCCGG GCGCCTCACC CGGTCTGCCC GCCCGCCACC CTCTCCCGCA 
GTGGACCTGC TGCAGATGCT GGAGATGAAC ATGGCCATCG CCTTCCCCGC AGCGCCCCTG 
CTGACCGTCA TCCTGGCCCT CGTGGGAATG GAGGCGATCA TGTCTGAGTT CTTCAACGAC 
ACCACCACGG CCTTCTACAT CATCCTCATC GTGTGGCTGG CTGACCAGTA TGACGCCATC 
TGCTGCCACA CCAACACCAG CAAGCGGCAC TGGCTTCGGT TCTTCTACCT GTACCACTTT 
GCCTTCTACG CTTACCACTA TCGCTTCAAC GGGCAGTACA GCAGCCTGGC ACTCGTCACC 
TCCTGGCTCT TCATCCAGCA TTCCATGATC TACTTCTTCC ACCACTACGA ACTACCTGCC 
ATCCTGCAGC AGATCCGTGT CCAGGAGATG CTGCTGCAGA CACCACCACT GGGCCCCGGC 
ACCCCCACAG CCCTGCCAGA CGACCTGAAC AACAATGGAG GCATCCCACC TATCACCCCC 
GACGCTGCCA GCCAGTCCCC TGCCCTGGGC CCTGGCTTGC CAGGTGCTGG CGGGGGCCCC 
GGGCCCATGG CTGAGGCACC TAGCTCCCTG GTGGCCGCAG CGGCCTCAGT GGCTGCAGCA 
GCCAGTGGTG ACCTGGGTTG GATGGCAGAG ACGGCTGCCA TCATCACAGA CGCCTCCTTC 
CTGTCTGGCC TGAGCGCCTC TCTCCTGGAC CGGCGGCCAG GCAGCCCCCT GAGCCCTAGT 
GGGGGGCTCC TACGGGCCCC CCAGGACAGT GCCCCTGCAA GCAACTCTCC AGGGTCTGAT 
GCAGTCTCTC AGACCACCGG CGGGGGCAGG CCCAACCCTG TGTCTACCGA TCTAGTGGAC 
ACACCGTCGG AGGTCGGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805577.1
CDS	432..1955
Translation	MEPDGLELEEEEEEELTMDMFGNSSIKFELDIEPKVFKPPGGPKALNDSQEFPFPETPPKAWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLAEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIGECPLWPSPLPLPHSGRLTRSARPPPSPAVDLLQMLEMNMAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRVQEMLLQTPPLGPGTPTALPDDLNNNGGIPPITPDAASQSPALGPGLPGAGGGPGPMAEAPSSLVAAAASVAAAASGDLGWMAETAAIITDASFLSGLSASLLDRRPGSPLSPSGGLLRAPQDSAPASNSPGSDAVSQTTGGGRPNPVSTDLVDTPSEVGS

Target ORF information:

RefSeq Version	XM_010807275.2
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807275.2

ORF Insert Sequence:

ATGGAGCCAG ATGGCCTGGA GCTAGAGGAG GAGGAGGAGG AGGAGCTGAC CATGGACATG 
TTCGGGAATA GCTCCATCAA GTTTGAACTG GACATCGAGC CCAAGGTGTT CAAGCCACCA 
GGTGGCCCCA AGGCCTTAAA TGACAGCCAG GAGTTCCCAT TCCCTGAGAC GCCCCCAAAA 
GCCTGGCCAC AAGACGAGTA CATCGTGGAG TACTCGCTGG AGTATGGCTT CCTGAGGTTG 
TCGCAGGCCA CACGCCAGCG GCTCAGCATC CCCGTAATGG TGGTCACGCT GGATCCCACG 
CGGGACCAGT GCTTTGGTGA CCGCTTCAGC CGCCTGCTGC TGGCTGAGTT CCTGGGCTAT 
GATGACATCC TCATGTCCAG CGTGAAGGGC CTGGCGGAGA ACGAGGAGAA CAAAGGCTTC 
CTGAGGAATG TGGTGTCCGG GGAGCACTAC CGCTTCGTGA GCATGTGGAT GGCCCGCACG 
TCCTACCTGG CCGCCTTCGT CATCATGGTC ATCTTTACCC TGAGCGTGTC CATGTTGCTG 
CGCTACTCCC ACCATCAGAT CTTCGTTTTC ATTGGTGAGT GTCCCCTCTG GCCATCTCCC 
TTGCCCCTCC CCCATTCCGG GCGCCTCACC CGGTCTGCCC GCCCGCCACC CTCTCCCGCA 
GTGGACCTGC TGCAGATGCT GGAGATGAAC ATGGCCATCG CCTTCCCCGC AGCGCCCCTG 
CTGACCGTCA TCCTGGCCCT CGTGGGAATG GAGGCGATCA TGTCTGAGTT CTTCAACGAC 
ACCACCACGG CCTTCTACAT CATCCTCATC GTGTGGCTGG CTGACCAGTA TGACGCCATC 
TGCTGCCACA CCAACACCAG CAAGCGGCAC TGGCTTCGGT TCTTCTACCT GTACCACTTT 
GCCTTCTACG CTTACCACTA TCGCTTCAAC GGGCAGTACA GCAGCCTGGC ACTCGTCACC 
TCCTGGCTCT TCATCCAGCA TTCCATGATC TACTTCTTCC ACCACTACGA ACTACCTGCC 
ATCCTGCAGC AGATCCGTGT CCAGGAGATG CTGCTGCAGA CACCACCACT GGGCCCCGGC 
ACCCCCACAG CCCTGCCAGA CGACCTGAAC AACAATGGAG GCATCCCACC TATCACCCCC 
GACGCTGCCA GCCAGTCCCC TGCCCTGGGC CCTGGCTTGC CAGGTGCTGG CGGGGGCCCC 
GGGCCCATGG CTGAGGCACC TAGCTCCCTG GTGGCCGCAG CGGCCTCAGT GGCTGCAGCA 
GCCAGTGGTG ACCTGGGTTG GATGGCAGAG ACGGCTGCCA TCATCACAGA CGCCTCCTTC 
CTGTCTGGCC TGAGCGCCTC TCTCCTGGAC CGGCGGCCAG GCAGCCCCCT GAGCCCTAGT 
GGGGGGCTCC TACGGGCCCC CCAGGACAGT GCCCCTGCAA GCAACTCTCC AGGGTCTGAT 
GCAGTCTCTC AGACCACCGG CGGGGGCAGG CCCAACCCTG TGTCTACCGA TCTAGTGGAC 
ACACCGTCGG AGGTCGGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM259 ( XM_015464251.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319737.1 Bos taurus transmembrane protein 259 (TMEM259), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa48955
Clone ID Related Accession (Same CDS sequence)	XM_010807275.2 , XM_015464251.1
Accession Version	XM_015464251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	membralin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAG ATGGCCTGGA GCTAGAGGAG GAGGAGGAGG AGGAGCTGAC CATGGACATG 
TTCGGGAATA GCTCCATCAA GTTTGAACTG GACATCGAGC CCAAGGTGTT CAAGCCACCA 
GGTGGCCCCA AGGCCTTAAA TGACAGCCAG GAGTTCCCAT TCCCTGAGAC GCCCCCAAAA 
GCCTGGCCAC AAGACGAGTA CATCGTGGAG TACTCGCTGG AGTATGGCTT CCTGAGGTTG 
TCGCAGGCCA CACGCCAGCG GCTCAGCATC CCCGTAATGG TGGTCACGCT GGATCCCACG 
CGGGACCAGT GCTTTGGTGA CCGCTTCAGC CGCCTGCTGC TGGCTGAGTT CCTGGGCTAT 
GATGACATCC TCATGTCCAG CGTGAAGGGC CTGGCGGAGA ACGAGGAGAA CAAAGGCTTC 
CTGAGGAATG TGGTGTCCGG GGAGCACTAC CGCTTCGTGA GCATGTGGAT GGCCCGCACG 
TCCTACCTGG CCGCCTTCGT CATCATGGTC ATCTTTACCC TGAGCGTGTC CATGTTGCTG 
CGCTACTCCC ACCATCAGAT CTTCGTTTTC ATTGGTGAGT GTCCCCTCTG GCCATCTCCC 
TTGCCCCTCC CCCATTCCGG GCGCCTCACC CGGTCTGCCC GCCCGCCACC CTCTCCCGCA 
GTGGACCTGC TGCAGATGCT GGAGATGAAC ATGGCCATCG CCTTCCCCGC AGCGCCCCTG 
CTGACCGTCA TCCTGGCCCT CGTGGGAATG GAGGCGATCA TGTCTGAGTT CTTCAACGAC 
ACCACCACGG CCTTCTACAT CATCCTCATC GTGTGGCTGG CTGACCAGTA TGACGCCATC 
TGCTGCCACA CCAACACCAG CAAGCGGCAC TGGCTTCGGT TCTTCTACCT GTACCACTTT 
GCCTTCTACG CTTACCACTA TCGCTTCAAC GGGCAGTACA GCAGCCTGGC ACTCGTCACC 
TCCTGGCTCT TCATCCAGCA TTCCATGATC TACTTCTTCC ACCACTACGA ACTACCTGCC 
ATCCTGCAGC AGATCCGTGT CCAGGAGATG CTGCTGCAGA CACCACCACT GGGCCCCGGC 
ACCCCCACAG CCCTGCCAGA CGACCTGAAC AACAATGGAG GCATCCCACC TATCACCCCC 
GACGCTGCCA GCCAGTCCCC TGCCCTGGGC CCTGGCTTGC CAGGTGCTGG CGGGGGCCCC 
GGGCCCATGG CTGAGGCACC TAGCTCCCTG GTGGCCGCAG CGGCCTCAGT GGCTGCAGCA 
GCCAGTGGTG ACCTGGGTTG GATGGCAGAG ACGGCTGCCA TCATCACAGA CGCCTCCTTC 
CTGTCTGGCC TGAGCGCCTC TCTCCTGGAC CGGCGGCCAG GCAGCCCCCT GAGCCCTAGT 
GGGGGGCTCC TACGGGCCCC CCAGGACAGT GCCCCTGCAA GCAACTCTCC AGGGTCTGAT 
GCAGTCTCTC AGACCACCGG CGGGGGCAGG CCCAACCCTG TGTCTACCGA TCTAGTGGAC 
ACACCGTCGG AGGTCGGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319737.1
CDS	432..1955
Translation	MEPDGLELEEEEEEELTMDMFGNSSIKFELDIEPKVFKPPGGPKALNDSQEFPFPETPPKAWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLAEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIGECPLWPSPLPLPHSGRLTRSARPPPSPAVDLLQMLEMNMAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRVQEMLLQTPPLGPGTPTALPDDLNNNGGIPPITPDAASQSPALGPGLPGAGGGPGPMAEAPSSLVAAAASVAAAASGDLGWMAETAAIITDASFLSGLSASLLDRRPGSPLSPSGGLLRAPQDSAPASNSPGSDAVSQTTGGGRPNPVSTDLVDTPSEVGS

Target ORF information:

RefSeq Version	XM_015464251.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464251.1

ORF Insert Sequence:

ATGGAGCCAG ATGGCCTGGA GCTAGAGGAG GAGGAGGAGG AGGAGCTGAC CATGGACATG 
TTCGGGAATA GCTCCATCAA GTTTGAACTG GACATCGAGC CCAAGGTGTT CAAGCCACCA 
GGTGGCCCCA AGGCCTTAAA TGACAGCCAG GAGTTCCCAT TCCCTGAGAC GCCCCCAAAA 
GCCTGGCCAC AAGACGAGTA CATCGTGGAG TACTCGCTGG AGTATGGCTT CCTGAGGTTG 
TCGCAGGCCA CACGCCAGCG GCTCAGCATC CCCGTAATGG TGGTCACGCT GGATCCCACG 
CGGGACCAGT GCTTTGGTGA CCGCTTCAGC CGCCTGCTGC TGGCTGAGTT CCTGGGCTAT 
GATGACATCC TCATGTCCAG CGTGAAGGGC CTGGCGGAGA ACGAGGAGAA CAAAGGCTTC 
CTGAGGAATG TGGTGTCCGG GGAGCACTAC CGCTTCGTGA GCATGTGGAT GGCCCGCACG 
TCCTACCTGG CCGCCTTCGT CATCATGGTC ATCTTTACCC TGAGCGTGTC CATGTTGCTG 
CGCTACTCCC ACCATCAGAT CTTCGTTTTC ATTGGTGAGT GTCCCCTCTG GCCATCTCCC 
TTGCCCCTCC CCCATTCCGG GCGCCTCACC CGGTCTGCCC GCCCGCCACC CTCTCCCGCA 
GTGGACCTGC TGCAGATGCT GGAGATGAAC ATGGCCATCG CCTTCCCCGC AGCGCCCCTG 
CTGACCGTCA TCCTGGCCCT CGTGGGAATG GAGGCGATCA TGTCTGAGTT CTTCAACGAC 
ACCACCACGG CCTTCTACAT CATCCTCATC GTGTGGCTGG CTGACCAGTA TGACGCCATC 
TGCTGCCACA CCAACACCAG CAAGCGGCAC TGGCTTCGGT TCTTCTACCT GTACCACTTT 
GCCTTCTACG CTTACCACTA TCGCTTCAAC GGGCAGTACA GCAGCCTGGC ACTCGTCACC 
TCCTGGCTCT TCATCCAGCA TTCCATGATC TACTTCTTCC ACCACTACGA ACTACCTGCC 
ATCCTGCAGC AGATCCGTGT CCAGGAGATG CTGCTGCAGA CACCACCACT GGGCCCCGGC 
ACCCCCACAG CCCTGCCAGA CGACCTGAAC AACAATGGAG GCATCCCACC TATCACCCCC 
GACGCTGCCA GCCAGTCCCC TGCCCTGGGC CCTGGCTTGC CAGGTGCTGG CGGGGGCCCC 
GGGCCCATGG CTGAGGCACC TAGCTCCCTG GTGGCCGCAG CGGCCTCAGT GGCTGCAGCA 
GCCAGTGGTG ACCTGGGTTG GATGGCAGAG ACGGCTGCCA TCATCACAGA CGCCTCCTTC 
CTGTCTGGCC TGAGCGCCTC TCTCCTGGAC CGGCGGCCAG GCAGCCCCCT GAGCCCTAGT 
GGGGGGCTCC TACGGGCCCC CCAGGACAGT GCCCCTGCAA GCAACTCTCC AGGGTCTGAT 
GCAGTCTCTC AGACCACCGG CGGGGGCAGG CCCAACCCTG TGTCTACCGA TCTAGTGGAC 
ACACCGTCGG AGGTCGGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM259 ( XM_024995105.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850873.1 Bos taurus transmembrane protein 259 (TMEM259), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa236632
Clone ID Related Accession (Same CDS sequence)	XM_024995105.1
Accession Version	XM_024995105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	membralin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGC ACGCGGCACC CGGGGCCCCG GGGCCTGGGC CCAACGGCGG TGGTGGCGGT 
GGCGGCGGTG GCGGCGGCGG CCAGGCCCCC GCGCGCGGGC CTCGCACCCC CAACCTCAAC 
CCCAATCCTC TCATCAACGT GCGCGACCGG CTTTTCCACG CGCTCTTCTT CAAGATGGCT 
GTCACCTACT CGCGCCTCTT CCCGCCCGCC TTCCGTCGAC TCTTCGAGTT TTTCGTGTTG 
CTCAAGGCCC TGTTCGTGCT CTTTGTCCTG GCCTACATCC ACATCGTCTT CTCCCGCTCA 
CCCATCAACT GCCTGGAGCA CGTGCGTGAC AAGTGGCCCC GTGAGGGCAT CCTGCGGGTA 
GAGGTGCAGC ACAACTCGAG CCGCGCGCCC GTCTTCCTGC AGTTCTGTGA TGGTGGCCAT 
GGCAGCTTCC CTGGCCTGGC CATGGAGCCA GATGGCCTGG AGCTAGAGGA GGAGGAGGAG 
GAGGAGCTGA CCATGGACAT GTTCGGGAAT AGCTCCATCA AGTTTGAACT GGACATCGAG 
CCCAAGGTGT TCAAGCCACC AGGTGGCCCC AAGGCCTTAA ATGACAGCCA GGAGTTCCCA 
TTCCCTGAGA CGCCCCCAAA AGCCTGGCCA CAAGACGAGT ACATCGTGGA GTACTCGCTG 
GAGTATGGCT TCCTGAGGTT GTCGCAGGCC ACACGCCAGC GGCTCAGCAT CCCCGTAATG 
GTGGTCACGC TGGATCCCAC GCGGGACCAG TGCTTTGGTG ACCGCTTCAG CCGCCTGCTG 
CTGGCTGAGT TCCTGGGCTA TGATGACATC CTCATGTCCA GCGTGAAGGG CCTGGCGGAG 
AACGAGGAGA ACAAAGGCTT CCTGAGGAAT GTGGTGTCCG GGGAGCACTA CCGCTTCGTG 
AGCATGTGGA TGGCCCGCAC GTCCTACCTG GCCGCCTTCG TCATCATGGT CATCTTTACC 
CTGAGCGTGT CCATGTTGCT GCGCTACTCC CACCATCAGA TCTTCGTTTT CATTGGTGAG 
TGTCCCCTCT GGCCATCTCC CTTGCCCCTC CCCCATTCCG GGCGCCTCAC CCGGTCTGCC 
CGCCCGCCAC CCTCTCCCGC AGTGGACCTG CTGCAGATGC TGGAGATGAA CATGGCCATC 
GCCTTCCCCG CAGCGCCCCT GCTGACCGTC ATCCTGGCCC TCGTGGGAAT GGAGGCGATC 
ATGTCTGAGT TCTTCAATGA CACCACCACG GCCTTCTACA TCATCCTCAT CGTGTGGCTG 
GCTGACCAGT ATGACGCCAT CTGCTGCCAC ACCAACACCA GCAAGCGGCA CTGGCTTCGG 
TTCTTCTACC TGTACCACTT TGCCTTCTAC GCTTACCACT ATCGCTTCAA CGGGCAGTAC 
AGCAGCCTGG CACTCGTCAC CTCCTGGCTC TTCATCCAGC ATTCCATGAT CTACTTCTTC 
CACCACTACG AACTACCTGC CATCCTGCAG CAGATCCGTG TCCAGGAGAT GCTGCTGCAG 
ACACCACCAC TGGGCCCCGG CACCCCCACA GCCCTGCCAG ACGACCTGAA CAACAATGGA 
GGCATCCCAC CTATCACCCC CGACGCTGCC AGCCAGTCCC CTGCCCTGGG CCCTGGCTTG 
CCAGGTGCTG GCGGGGGCCC CGGGCCCATG GCTGAGGCAC CTAGCTCCCT GGTGGCCGCA 
GCGGCCTCAG TGGCTGCAGC AGCCAGTGGT GACCTGGGTT GGATGGCAGA GACGGCTGCC 
ATCATCACAG ACGCCTCCTT CCTGTCTGGC CTGAGCGCCT CTCTCCTGGA CCGGCGGCCA 
GGCAGCCCCC TGAGCCCTAG TGGGGGGCTC CTACGGGCCC CCCAGGACAG TGCCCCTGCA 
AGCAACTCTC CAGGGTCTGA TGCAGTCTCT CAGACCACCG GCGGGGGCAG GCCCAACCCT 
GTGTCTACCG ATCTAGTGGA CACACCGTCG GAGGTCGGCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850873.1
CDS	190..2154
Translation	MSEHAAPGAPGPGPNGGGGGGGGGGGGQAPARGPRTPNLNPNPLINVRDRLFHALFFKMAVTYSRLFPPAFRRLFEFFVLLKALFVLFVLAYIHIVFSRSPINCLEHVRDKWPREGILRVEVQHNSSRAPVFLQFCDGGHGSFPGLAMEPDGLELEEEEEEELTMDMFGNSSIKFELDIEPKVFKPPGGPKALNDSQEFPFPETPPKAWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLAEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIGECPLWPSPLPLPHSGRLTRSARPPPSPAVDLLQMLEMNMAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRVQEMLLQTPPLGPGTPTALPDDLNNNGGIPPITPDAASQSPALGPGLPGAGGGPGPMAEAPSSLVAAAASVAAAASGDLGWMAETAAIITDASFLSGLSASLLDRRPGSPLSPSGGLLRAPQDSAPASNSPGSDAVSQTTGGGRPNPVSTDLVDTPSEVGS

Target ORF information:

RefSeq Version	XM_024995105.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995105.1

ORF Insert Sequence:

ATGTCGGAGC ACGCGGCACC CGGGGCCCCG GGGCCTGGGC CCAACGGCGG TGGTGGCGGT 
GGCGGCGGTG GCGGCGGCGG CCAGGCCCCC GCGCGCGGGC CTCGCACCCC CAACCTCAAC 
CCCAATCCTC TCATCAACGT GCGCGACCGG CTTTTCCACG CGCTCTTCTT CAAGATGGCT 
GTCACCTACT CGCGCCTCTT CCCGCCCGCC TTCCGTCGAC TCTTCGAGTT TTTCGTGTTG 
CTCAAGGCCC TGTTCGTGCT CTTTGTCCTG GCCTACATCC ACATCGTCTT CTCCCGCTCA 
CCCATCAACT GCCTGGAGCA CGTGCGTGAC AAGTGGCCCC GTGAGGGCAT CCTGCGGGTA 
GAGGTGCAGC ACAACTCGAG CCGCGCGCCC GTCTTCCTGC AGTTCTGTGA TGGTGGCCAT 
GGCAGCTTCC CTGGCCTGGC CATGGAGCCA GATGGCCTGG AGCTAGAGGA GGAGGAGGAG 
GAGGAGCTGA CCATGGACAT GTTCGGGAAT AGCTCCATCA AGTTTGAACT GGACATCGAG 
CCCAAGGTGT TCAAGCCACC AGGTGGCCCC AAGGCCTTAA ATGACAGCCA GGAGTTCCCA 
TTCCCTGAGA CGCCCCCAAA AGCCTGGCCA CAAGACGAGT ACATCGTGGA GTACTCGCTG 
GAGTATGGCT TCCTGAGGTT GTCGCAGGCC ACACGCCAGC GGCTCAGCAT CCCCGTAATG 
GTGGTCACGC TGGATCCCAC GCGGGACCAG TGCTTTGGTG ACCGCTTCAG CCGCCTGCTG 
CTGGCTGAGT TCCTGGGCTA TGATGACATC CTCATGTCCA GCGTGAAGGG CCTGGCGGAG 
AACGAGGAGA ACAAAGGCTT CCTGAGGAAT GTGGTGTCCG GGGAGCACTA CCGCTTCGTG 
AGCATGTGGA TGGCCCGCAC GTCCTACCTG GCCGCCTTCG TCATCATGGT CATCTTTACC 
CTGAGCGTGT CCATGTTGCT GCGCTACTCC CACCATCAGA TCTTCGTTTT CATTGGTGAG 
TGTCCCCTCT GGCCATCTCC CTTGCCCCTC CCCCATTCCG GGCGCCTCAC CCGGTCTGCC 
CGCCCGCCAC CCTCTCCCGC AGTGGACCTG CTGCAGATGC TGGAGATGAA CATGGCCATC 
GCCTTCCCCG CAGCGCCCCT GCTGACCGTC ATCCTGGCCC TCGTGGGAAT GGAGGCGATC 
ATGTCTGAGT TCTTCAATGA CACCACCACG GCCTTCTACA TCATCCTCAT CGTGTGGCTG 
GCTGACCAGT ATGACGCCAT CTGCTGCCAC ACCAACACCA GCAAGCGGCA CTGGCTTCGG 
TTCTTCTACC TGTACCACTT TGCCTTCTAC GCTTACCACT ATCGCTTCAA CGGGCAGTAC 
AGCAGCCTGG CACTCGTCAC CTCCTGGCTC TTCATCCAGC ATTCCATGAT CTACTTCTTC 
CACCACTACG AACTACCTGC CATCCTGCAG CAGATCCGTG TCCAGGAGAT GCTGCTGCAG 
ACACCACCAC TGGGCCCCGG CACCCCCACA GCCCTGCCAG ACGACCTGAA CAACAATGGA 
GGCATCCCAC CTATCACCCC CGACGCTGCC AGCCAGTCCC CTGCCCTGGG CCCTGGCTTG 
CCAGGTGCTG GCGGGGGCCC CGGGCCCATG GCTGAGGCAC CTAGCTCCCT GGTGGCCGCA 
GCGGCCTCAG TGGCTGCAGC AGCCAGTGGT GACCTGGGTT GGATGGCAGA GACGGCTGCC 
ATCATCACAG ACGCCTCCTT CCTGTCTGGC CTGAGCGCCT CTCTCCTGGA CCGGCGGCCA 
GGCAGCCCCC TGAGCCCTAG TGGGGGGCTC CTACGGGCCC CCCAGGACAG TGCCCCTGCA 
AGCAACTCTC CAGGGTCTGA TGCAGTCTCT CAGACCACCG GCGGGGGCAG GCCCAACCCT 
GTGTCTACCG ATCTAGTGGA CACACCGTCG GAGGTCGGCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM259 ( XM_024995106.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850874.1 Bos taurus transmembrane protein 259 (TMEM259), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa236633
Clone ID Related Accession (Same CDS sequence)	XM_024995106.1
Accession Version	XM_024995106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	membralin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGC ACGCGGCACC CGGGGCCCCG GGGCCTGGGC CCAACGGCGG TGGTGGCGGT 
GGCGGCGGTG GCGGCGGCGG CCAGGCCCCC GCGCGCGGGC CTCGCACCCC CAACCTCAAC 
CCCAATCCTC TCATCAACGT GCGCGACCGG CTTTTCCACG CGCTCTTCTT CAAGATGGCT 
GTCACCTACT CGCGCCTCTT CCCGCCCGCC TTCCGTCGAC TCTTCGAGTT TTTCGTGTTG 
CTCAAGGCCC TGTTCGTGCT CTTTGTCCTG GCCTACATCC ACATCGTCTT CTCCCGCTCA 
CCCATCAACT GCCTGGAGCA CGTGCGTGAC AAGTGGCCCC GTGAGGGCAT CCTGCGGGTA 
GAGGTGCAGC ACAACTCGAG CCGCGCGCCC GTCTTCCTGC AGTTCTGTGA TGGTGGCCAT 
GGCAGCTTCC CTGGCCTGGC CATGGAGCCA GATGGCCTGG AGCTAGAGGA GGAGGAGGAG 
GAGGAGCTGA CCATGGACAT GTTCGGGAAT AGCTCCATCA AGTTTGAACT GGACATCGAG 
CCCAAGGTGT TCAAGCCACC AGGTGGCCCC AAGGCCTTAA ATGACAGCCA GGAGTTCCCA 
TTCCCTGAGA CGCCCCCAAA AGCCTGGCCA CAAGACGAGT ACATCGTGGA GTACTCGCTG 
GAGTATGGCT TCCTGAGGTT GTCGCAGGCC ACACGCCAGC GGCTCAGCAT CCCCGTAATG 
GTGGTCACGC TGGATCCCAC GCGGGACCAG TGCTTTGGTG ACCGCTTCAG CCGCCTGCTG 
CTGGCTGAGT TCCTGGGCTA TGATGACATC CTCATGTCCA GCGTGAAGGG CCTGGCGGAG 
AACGAGGAGA ACAAAGGCTT CCTGAGGAAT GTGGTGTCCG GGGAGCACTA CCGCTTCGTG 
AGCATGTGGA TGGCCCGCAC GTCCTACCTG GCCGCCTTCG TCATCATGGT CATCTTTACC 
CTGAGCGTGT CCATGTTGCT GCGCTACTCC CACCATCAGA TCTTCGTTTT CATTGTGGAC 
CTGCTGCAGA TGCTGGAGAT GAACATGGCC ATCGCCTTCC CCGCAGCGCC CCTGCTGACC 
GTCATCCTGG CCCTCGTGGG AATGGAGGCG ATCATGTCTG AGTTCTTCAA TGACACCACC 
ACGGCCTTCT ACATCATCCT CATCGTGTGG CTGGCTGACC AGTATGACGC CATCTGCTGC 
CACACCAACA CCAGCAAGCG GCACTGGCTT CGGTGGGCAC CAGTGGCCAT GTGGTCCGGG 
GTGGAGGCAG CCGGGTGGGC AGCACCTCAT TGTTCCCCTT GCAGGTTCTT CTACCTGTAC 
CACTTTGCCT TCTACGCTTA CCACTATCGC TTCAACGGGC AGTACAGCAG CCTGGCACTC 
GTCACCTCCT GGCTCTTCAT CCAGCATTCC ATGATCTACT TCTTCCACCA CTACGAACTA 
CCTGCCATCC TGCAGCAGAT CCGTGTCCAG GAGATGCTGC TGCAGACACC ACCACTGGGC 
CCCGGCACCC CCACAGCCCT GCCAGACGAC CTGAACAACA ATGGAGGCAT CCCACCTATC 
ACCCCCGACG CTGCCAGCCA GTCCCCTGCC CTGGGCCCTG GCTTGCCAGG TGCTGGCGGG 
GGCCCCGGGC CCATGGCTGA GGCACCTAGC TCCCTGGTGG CCGCAGCGGC CTCAGTGGCT 
GCAGCAGCCA GTGGTGACCT GGGTTGGATG GCAGAGACGG CTGCCATCAT CACAGACGCC 
TCCTTCCTGT CTGGCCTGAG CGCCTCTCTC CTGGACCGGC GGCCAGGCAG CCCCCTGAGC 
CCTAGTGGGG GGCTCCTACG GGCCCCCCAG GACAGTGCCC CTGCAAGCAA CTCTCCAGGG 
TCTGATGCAG TCTCTCAGAC CACCGGCGGG GGCAGGCCCA ACCCTGTGTC TACCGATCTA 
GTGGACACAC CGTCGGAGGT CGGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850874.1
CDS	1..1950
Translation	MSEHAAPGAPGPGPNGGGGGGGGGGGGQAPARGPRTPNLNPNPLINVRDRLFHALFFKMAVTYSRLFPPAFRRLFEFFVLLKALFVLFVLAYIHIVFSRSPINCLEHVRDKWPREGILRVEVQHNSSRAPVFLQFCDGGHGSFPGLAMEPDGLELEEEEEEELTMDMFGNSSIKFELDIEPKVFKPPGGPKALNDSQEFPFPETPPKAWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLAEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIVDLLQMLEMNMAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRWAPVAMWSGVEAAGWAAPHCSPCRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRVQEMLLQTPPLGPGTPTALPDDLNNNGGIPPITPDAASQSPALGPGLPGAGGGPGPMAEAPSSLVAAAASVAAAASGDLGWMAETAAIITDASFLSGLSASLLDRRPGSPLSPSGGLLRAPQDSAPASNSPGSDAVSQTTGGGRPNPVSTDLVDTPSEVGS

Target ORF information:

RefSeq Version	XM_024995106.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995106.1

ORF Insert Sequence:

ATGTCGGAGC ACGCGGCACC CGGGGCCCCG GGGCCTGGGC CCAACGGCGG TGGTGGCGGT 
GGCGGCGGTG GCGGCGGCGG CCAGGCCCCC GCGCGCGGGC CTCGCACCCC CAACCTCAAC 
CCCAATCCTC TCATCAACGT GCGCGACCGG CTTTTCCACG CGCTCTTCTT CAAGATGGCT 
GTCACCTACT CGCGCCTCTT CCCGCCCGCC TTCCGTCGAC TCTTCGAGTT TTTCGTGTTG 
CTCAAGGCCC TGTTCGTGCT CTTTGTCCTG GCCTACATCC ACATCGTCTT CTCCCGCTCA 
CCCATCAACT GCCTGGAGCA CGTGCGTGAC AAGTGGCCCC GTGAGGGCAT CCTGCGGGTA 
GAGGTGCAGC ACAACTCGAG CCGCGCGCCC GTCTTCCTGC AGTTCTGTGA TGGTGGCCAT 
GGCAGCTTCC CTGGCCTGGC CATGGAGCCA GATGGCCTGG AGCTAGAGGA GGAGGAGGAG 
GAGGAGCTGA CCATGGACAT GTTCGGGAAT AGCTCCATCA AGTTTGAACT GGACATCGAG 
CCCAAGGTGT TCAAGCCACC AGGTGGCCCC AAGGCCTTAA ATGACAGCCA GGAGTTCCCA 
TTCCCTGAGA CGCCCCCAAA AGCCTGGCCA CAAGACGAGT ACATCGTGGA GTACTCGCTG 
GAGTATGGCT TCCTGAGGTT GTCGCAGGCC ACACGCCAGC GGCTCAGCAT CCCCGTAATG 
GTGGTCACGC TGGATCCCAC GCGGGACCAG TGCTTTGGTG ACCGCTTCAG CCGCCTGCTG 
CTGGCTGAGT TCCTGGGCTA TGATGACATC CTCATGTCCA GCGTGAAGGG CCTGGCGGAG 
AACGAGGAGA ACAAAGGCTT CCTGAGGAAT GTGGTGTCCG GGGAGCACTA CCGCTTCGTG 
AGCATGTGGA TGGCCCGCAC GTCCTACCTG GCCGCCTTCG TCATCATGGT CATCTTTACC 
CTGAGCGTGT CCATGTTGCT GCGCTACTCC CACCATCAGA TCTTCGTTTT CATTGTGGAC 
CTGCTGCAGA TGCTGGAGAT GAACATGGCC ATCGCCTTCC CCGCAGCGCC CCTGCTGACC 
GTCATCCTGG CCCTCGTGGG AATGGAGGCG ATCATGTCTG AGTTCTTCAA TGACACCACC 
ACGGCCTTCT ACATCATCCT CATCGTGTGG CTGGCTGACC AGTATGACGC CATCTGCTGC 
CACACCAACA CCAGCAAGCG GCACTGGCTT CGGTGGGCAC CAGTGGCCAT GTGGTCCGGG 
GTGGAGGCAG CCGGGTGGGC AGCACCTCAT TGTTCCCCTT GCAGGTTCTT CTACCTGTAC 
CACTTTGCCT TCTACGCTTA CCACTATCGC TTCAACGGGC AGTACAGCAG CCTGGCACTC 
GTCACCTCCT GGCTCTTCAT CCAGCATTCC ATGATCTACT TCTTCCACCA CTACGAACTA 
CCTGCCATCC TGCAGCAGAT CCGTGTCCAG GAGATGCTGC TGCAGACACC ACCACTGGGC 
CCCGGCACCC CCACAGCCCT GCCAGACGAC CTGAACAACA ATGGAGGCAT CCCACCTATC 
ACCCCCGACG CTGCCAGCCA GTCCCCTGCC CTGGGCCCTG GCTTGCCAGG TGCTGGCGGG 
GGCCCCGGGC CCATGGCTGA GGCACCTAGC TCCCTGGTGG CCGCAGCGGC CTCAGTGGCT 
GCAGCAGCCA GTGGTGACCT GGGTTGGATG GCAGAGACGG CTGCCATCAT CACAGACGCC 
TCCTTCCTGT CTGGCCTGAG CGCCTCTCTC CTGGACCGGC GGCCAGGCAG CCCCCTGAGC 
CCTAGTGGGG GGCTCCTACG GGCCCCCCAG GACAGTGCCC CTGCAAGCAA CTCTCCAGGG 
TCTGATGCAG TCTCTCAGAC CACCGGCGGG GGCAGGCCCA ACCCTGTGTC TACCGATCTA 
GTGGACACAC CGTCGGAGGT CGGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM259 ( XM_024995107.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850875.1 Bos taurus transmembrane protein 259 (TMEM259), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa236634
Clone ID Related Accession (Same CDS sequence)	XM_024995107.1
Accession Version	XM_024995107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	membralin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGC ACGCGGCACC CGGGGCCCCG GGGCCTGGGC CCAACGGCGG TGGTGGCGGT 
GGCGGCGGTG GCGGCGGCGG CCAGGCCCCC GCGCGCGGGC CTCGCACCCC CAACCTCAAC 
CCCAATCCTC TCATCAACGT GCGCGACCGG CTTTTCCACG CGCTCTTCTT CAAGATGGCT 
GTCACCTACT CGCGCCTCTT CCCGCCCGCC TTCCGTCGAC TCTTCGAGTT TTTCGTGTTG 
CTCAAGGCCC TGTTCGTGCT CTTTGTCCTG GCCTACATCC ACATCGTCTT CTCCCGCTCA 
CCCATCAACT GCCTGGAGCA CGTGCGTGAC AAGTGGCCCC GTGAGGGCAT CCTGCGGGTA 
GAGGTGCAGC ACAACTCGAG CCGCGCGCCC GTCTTCCTGC AGTTCTGTGA TGGTGGCCAT 
GGCAGCTTCC CTGGCCTGGC CATGGAGCCA GATGGCCTGG AGCTAGAGGA GGAGGAGGAG 
GAGGAGCTGA CCATGGACAT GTTCGGGAAT AGCTCCATCA AGTTTGAACT GGACATCGAG 
CCCAAGGTGT TCAAGCCACC AGGTGGCCCC AAGGCCTTAA ATGACAGCCA GGAGTTCCCA 
TTCCCTGAGA CGCCCCCAAA AGCCTGGCCA CAAGACGAGT ACATCGTGGA GTACTCGCTG 
GAGTATGGCT TCCTGAGGTT GTCGCAGGCC ACACGCCAGC GGCTCAGCAT CCCCGTAATG 
GTGGTCACGC TGGATCCCAC GCGGGACCAG TGCTTTGGTG ACCGCTTCAG CCGCCTGCTG 
CTGGCTGAGT TCCTGGGCTA TGATGACATC CTCATGTCCA GCGTGAAGGG CCTGGCGGAG 
AACGAGGAGA ACAAAGGCTT CCTGAGGAAT GTGGTGTCCG GGGAGCACTA CCGCTTCGTG 
AGCATGTGGA TGGCCCGCAC GTCCTACCTG GCCGCCTTCG TCATCATGGT CATCTTTACC 
CTGAGCGTGT CCATGTTGCT GCGCTACTCC CACCATCAGA TCTTCGTTTT CATTGTGGAC 
CTGCTGCAGA TGCTGGAGAT GAACATGGCC ATCGCCTTCC CCGCAGCGCC CCTGCTGACC 
GTCATCCTGG CCCTCGTGGG AATGGAGGCG ATCATGTCTG AGTTCTTCAA TGACACCACC 
ACGGCCTTCT ACATCATCCT CATCGTGTGG CTGGCTGACC AGTATGACGC CATCTGCTGC 
CACACCAACA CCAGCAAGCG GCACTGGCTT CGGTTCTTCT ACCTGTACCA CTTTGCCTTC 
TACGCTTACC ACTATCGCTT CAACGGGCAG TACAGCAGCC TGGCACTCGT CACCTCCTGG 
CTCTTCATCC AGCATTCCAT GATCTACTTC TTCCACCACT ACGAACTACC TGCCATCCTG 
CAGCAGATCC GTGTCCAGGA GATGCTGCTG CAGACACCAC CACTGGGCCC CGGCACCCCC 
ACAGCCCTGC CAGACGACCT GAACAACAAT GGAGGCATCC CACCTATCAC CCCCGACGCT 
GCCAGCCAGT CCCCTGCCCT GGGCCCTGGC TTGCCAGGTG CTGGCGGGGG CCCCGGGCCC 
ATGGCTGAGG CACCTAGCTC CCTGGTGGCC GCAGCGGCCT CAGTGGCTGC AGCAGCCAGT 
GGTGACCTGG GTTGGATGGC AGAGACGGCT GCCATCATCA CAGACGCCTC CTTCCTGTCT 
GGCCTGAGCG CCTCTCTCCT GGACCGGCGG CCAGGCAGCC CCCTGAGCCC TAGTGGGGGG 
CTCCTACGGG CCCCCCAGGA CAGTGCCCCT GCAAGCAACT CTCCAGGGTC TGATGCAGTC 
TCTCAGACCA CCGGCGGGGG CAGGCCCAAC CCTGTGTCTA CCGATCTAGT GGACACACCG 
TCGGAGGTCG GCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850875.1
CDS	1..1878
Translation	MSEHAAPGAPGPGPNGGGGGGGGGGGGQAPARGPRTPNLNPNPLINVRDRLFHALFFKMAVTYSRLFPPAFRRLFEFFVLLKALFVLFVLAYIHIVFSRSPINCLEHVRDKWPREGILRVEVQHNSSRAPVFLQFCDGGHGSFPGLAMEPDGLELEEEEEEELTMDMFGNSSIKFELDIEPKVFKPPGGPKALNDSQEFPFPETPPKAWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLAEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIVDLLQMLEMNMAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRVQEMLLQTPPLGPGTPTALPDDLNNNGGIPPITPDAASQSPALGPGLPGAGGGPGPMAEAPSSLVAAAASVAAAASGDLGWMAETAAIITDASFLSGLSASLLDRRPGSPLSPSGGLLRAPQDSAPASNSPGSDAVSQTTGGGRPNPVSTDLVDTPSEVGS

Target ORF information:

RefSeq Version	XM_024995107.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995107.1

ORF Insert Sequence:

ATGTCGGAGC ACGCGGCACC CGGGGCCCCG GGGCCTGGGC CCAACGGCGG TGGTGGCGGT 
GGCGGCGGTG GCGGCGGCGG CCAGGCCCCC GCGCGCGGGC CTCGCACCCC CAACCTCAAC 
CCCAATCCTC TCATCAACGT GCGCGACCGG CTTTTCCACG CGCTCTTCTT CAAGATGGCT 
GTCACCTACT CGCGCCTCTT CCCGCCCGCC TTCCGTCGAC TCTTCGAGTT TTTCGTGTTG 
CTCAAGGCCC TGTTCGTGCT CTTTGTCCTG GCCTACATCC ACATCGTCTT CTCCCGCTCA 
CCCATCAACT GCCTGGAGCA CGTGCGTGAC AAGTGGCCCC GTGAGGGCAT CCTGCGGGTA 
GAGGTGCAGC ACAACTCGAG CCGCGCGCCC GTCTTCCTGC AGTTCTGTGA TGGTGGCCAT 
GGCAGCTTCC CTGGCCTGGC CATGGAGCCA GATGGCCTGG AGCTAGAGGA GGAGGAGGAG 
GAGGAGCTGA CCATGGACAT GTTCGGGAAT AGCTCCATCA AGTTTGAACT GGACATCGAG 
CCCAAGGTGT TCAAGCCACC AGGTGGCCCC AAGGCCTTAA ATGACAGCCA GGAGTTCCCA 
TTCCCTGAGA CGCCCCCAAA AGCCTGGCCA CAAGACGAGT ACATCGTGGA GTACTCGCTG 
GAGTATGGCT TCCTGAGGTT GTCGCAGGCC ACACGCCAGC GGCTCAGCAT CCCCGTAATG 
GTGGTCACGC TGGATCCCAC GCGGGACCAG TGCTTTGGTG ACCGCTTCAG CCGCCTGCTG 
CTGGCTGAGT TCCTGGGCTA TGATGACATC CTCATGTCCA GCGTGAAGGG CCTGGCGGAG 
AACGAGGAGA ACAAAGGCTT CCTGAGGAAT GTGGTGTCCG GGGAGCACTA CCGCTTCGTG 
AGCATGTGGA TGGCCCGCAC GTCCTACCTG GCCGCCTTCG TCATCATGGT CATCTTTACC 
CTGAGCGTGT CCATGTTGCT GCGCTACTCC CACCATCAGA TCTTCGTTTT CATTGTGGAC 
CTGCTGCAGA TGCTGGAGAT GAACATGGCC ATCGCCTTCC CCGCAGCGCC CCTGCTGACC 
GTCATCCTGG CCCTCGTGGG AATGGAGGCG ATCATGTCTG AGTTCTTCAA TGACACCACC 
ACGGCCTTCT ACATCATCCT CATCGTGTGG CTGGCTGACC AGTATGACGC CATCTGCTGC 
CACACCAACA CCAGCAAGCG GCACTGGCTT CGGTTCTTCT ACCTGTACCA CTTTGCCTTC 
TACGCTTACC ACTATCGCTT CAACGGGCAG TACAGCAGCC TGGCACTCGT CACCTCCTGG 
CTCTTCATCC AGCATTCCAT GATCTACTTC TTCCACCACT ACGAACTACC TGCCATCCTG 
CAGCAGATCC GTGTCCAGGA GATGCTGCTG CAGACACCAC CACTGGGCCC CGGCACCCCC 
ACAGCCCTGC CAGACGACCT GAACAACAAT GGAGGCATCC CACCTATCAC CCCCGACGCT 
GCCAGCCAGT CCCCTGCCCT GGGCCCTGGC TTGCCAGGTG CTGGCGGGGG CCCCGGGCCC 
ATGGCTGAGG CACCTAGCTC CCTGGTGGCC GCAGCGGCCT CAGTGGCTGC AGCAGCCAGT 
GGTGACCTGG GTTGGATGGC AGAGACGGCT GCCATCATCA CAGACGCCTC CTTCCTGTCT 
GGCCTGAGCG CCTCTCTCCT GGACCGGCGG CCAGGCAGCC CCCTGAGCCC TAGTGGGGGG 
CTCCTACGGG CCCCCCAGGA CAGTGCCCCT GCAAGCAACT CTCCAGGGTC TGATGCAGTC 
TCTCAGACCA CCGGCGGGGG CAGGCCCAAC CCTGTGTCTA CCGATCTAGT GGACACACCG 
TCGGAGGTCG GCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM259 ( XM_024995108.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850876.1 Bos taurus transmembrane protein 259 (TMEM259), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa236635
Clone ID Related Accession (Same CDS sequence)	XM_024995108.1
Accession Version	XM_024995108.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	membralin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGC ACGCGGCACC CGGGGCCCCG GGGCCTGGGC CCAACGGCGG TGGTGGCGGT 
GGCGGCGGTG GCGGCGGCGG CCAGGCCCCC GCGCGCGGGC CTCGCACCCC CAACCTCAAC 
CCCAATCCTC TCATCAACGT GCGCGACCGG CTTTTCCACG CGCTCTTCTT CAAGATGGCT 
GTCACCTACT CGCGCCTCTT CCCGCCCGCC TTCCGTCGAC TCTTCGAGTT TTTCGTGTTG 
CTCAAGGCCC TGTTCGTGCT CTTTGTCCTG GCCTACATCC ACATCGTCTT CTCCCGCTCA 
CCCATCAACT GCCTGGAGCA CGTGCGTGAC AAGTGGCCCC GTGAGGGCAT CCTGCGGGTA 
GAGGTGCAGC ACAACTCGAG CCGCGCGCCC GTCTTCCTGC AGTTCTGTGA TGGTGGCCAT 
GGCAGCTTCC CTGGCCTGGC CATGGAGCCA GATGGCCTGG AGCTAGAGGA GGAGGAGGAG 
GAGGAGCTGA CCATGGACAT GTTCGGGAAT AGCTCCATCA AGTTTGAACT GGACATCGAG 
CCCAAGGTGT TCAAGCCACC AGGTGGCCCC AAGGCCTTAA ATGACAGCCA GGAGTTCCCA 
TTCCCTGAGA CGCCCCCAAA AGCCTGGCCA CAAGACGAGT ACATCGTGGA GTACTCGCTG 
GAGTATGGCT TCCTGAGGTT GTCGCAGGCC ACACGCCAGC GGCTCAGCAT CCCCGTAATG 
GTGGTCACGC TGGATCCCAC GCGGGACCAG TGCTTTGGTG ACCGCTTCAG CCGCCTGCTG 
CTGGCTGAGT TCCTGGGCTA TGATGACATC CTCATGTCCA GCGTGAAGGG CCTGGCGGAG 
AACGAGGAGA ACAAAGGCTT CCTGAGGAAT GTGGTGTCCG GGGAGCACTA CCGCTTCGTG 
AGCATGTGGA TGGCCCGCAC GTCCTACCTG GCCGCCTTCG TCATCATGGT CATCTTTACC 
CTGAGCGTGT CCATGTTGCT GCGCTACTCC CACCATCAGA TCTTCGTTTT CATTGACCTG 
CTGCAGATGC TGGAGATGAA CATGGCCATC GCCTTCCCCG CAGCGCCCCT GCTGACCGTC 
ATCCTGGCCC TCGTGGGAAT GGAGGCGATC ATGTCTGAGT TCTTCAATGA CACCACCACG 
GCCTTCTACA TCATCCTCAT CGTGTGGCTG GCTGACCAGT ATGACGCCAT CTGCTGCCAC 
ACCAACACCA GCAAGCGGCA CTGGCTTCGG TTCTTCTACC TGTACCACTT TGCCTTCTAC 
GCTTACCACT ATCGCTTCAA CGGGCAGTAC AGCAGCCTGG CACTCGTCAC CTCCTGGCTC 
TTCATCCAGC ATTCCATGAT CTACTTCTTC CACCACTACG AACTACCTGC CATCCTGCAG 
CAGATCCGTG TCCAGGAGAT GCTGCTGCAG ACACCACCAC TGGGCCCCGG CACCCCCACA 
GCCCTGCCAG ACGACCTGAA CAACAATGGA GGCATCCCAC CTATCACCCC CGACGCTGCC 
AGCCAGTCCC CTGCCCTGGG CCCTGGCTTG CCAGGTGCTG GCGGGGGCCC CGGGCCCATG 
GCTGAGGCAC CTAGCTCCCT GGTGGCCGCA GCGGCCTCAG TGGCTGCAGC AGCCAGTGGT 
GACCTGGGTT GGATGGCAGA GACGGCTGCC ATCATCACAG ACGCCTCCTT CCTGTCTGGC 
CTGAGCGCCT CTCTCCTGGA CCGGCGGCCA GGCAGCCCCC TGAGCCCTAG TGGGGGGCTC 
CTACGGGCCC CCCAGGACAG TGCCCCTGCA AGCAACTCTC CAGGGTCTGA TGCAGTCTCT 
CAGACCACCG GCGGGGGCAG GCCCAACCCT GTGTCTACCG ATCTAGTGGA CACACCGTCG 
GAGGTCGGCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850876.1
CDS	190..2064
Translation	MSEHAAPGAPGPGPNGGGGGGGGGGGGQAPARGPRTPNLNPNPLINVRDRLFHALFFKMAVTYSRLFPPAFRRLFEFFVLLKALFVLFVLAYIHIVFSRSPINCLEHVRDKWPREGILRVEVQHNSSRAPVFLQFCDGGHGSFPGLAMEPDGLELEEEEEEELTMDMFGNSSIKFELDIEPKVFKPPGGPKALNDSQEFPFPETPPKAWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLAEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIDLLQMLEMNMAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRVQEMLLQTPPLGPGTPTALPDDLNNNGGIPPITPDAASQSPALGPGLPGAGGGPGPMAEAPSSLVAAAASVAAAASGDLGWMAETAAIITDASFLSGLSASLLDRRPGSPLSPSGGLLRAPQDSAPASNSPGSDAVSQTTGGGRPNPVSTDLVDTPSEVGS

Target ORF information:

RefSeq Version	XM_024995108.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995108.1

ORF Insert Sequence:

ATGTCGGAGC ACGCGGCACC CGGGGCCCCG GGGCCTGGGC CCAACGGCGG TGGTGGCGGT 
GGCGGCGGTG GCGGCGGCGG CCAGGCCCCC GCGCGCGGGC CTCGCACCCC CAACCTCAAC 
CCCAATCCTC TCATCAACGT GCGCGACCGG CTTTTCCACG CGCTCTTCTT CAAGATGGCT 
GTCACCTACT CGCGCCTCTT CCCGCCCGCC TTCCGTCGAC TCTTCGAGTT TTTCGTGTTG 
CTCAAGGCCC TGTTCGTGCT CTTTGTCCTG GCCTACATCC ACATCGTCTT CTCCCGCTCA 
CCCATCAACT GCCTGGAGCA CGTGCGTGAC AAGTGGCCCC GTGAGGGCAT CCTGCGGGTA 
GAGGTGCAGC ACAACTCGAG CCGCGCGCCC GTCTTCCTGC AGTTCTGTGA TGGTGGCCAT 
GGCAGCTTCC CTGGCCTGGC CATGGAGCCA GATGGCCTGG AGCTAGAGGA GGAGGAGGAG 
GAGGAGCTGA CCATGGACAT GTTCGGGAAT AGCTCCATCA AGTTTGAACT GGACATCGAG 
CCCAAGGTGT TCAAGCCACC AGGTGGCCCC AAGGCCTTAA ATGACAGCCA GGAGTTCCCA 
TTCCCTGAGA CGCCCCCAAA AGCCTGGCCA CAAGACGAGT ACATCGTGGA GTACTCGCTG 
GAGTATGGCT TCCTGAGGTT GTCGCAGGCC ACACGCCAGC GGCTCAGCAT CCCCGTAATG 
GTGGTCACGC TGGATCCCAC GCGGGACCAG TGCTTTGGTG ACCGCTTCAG CCGCCTGCTG 
CTGGCTGAGT TCCTGGGCTA TGATGACATC CTCATGTCCA GCGTGAAGGG CCTGGCGGAG 
AACGAGGAGA ACAAAGGCTT CCTGAGGAAT GTGGTGTCCG GGGAGCACTA CCGCTTCGTG 
AGCATGTGGA TGGCCCGCAC GTCCTACCTG GCCGCCTTCG TCATCATGGT CATCTTTACC 
CTGAGCGTGT CCATGTTGCT GCGCTACTCC CACCATCAGA TCTTCGTTTT CATTGACCTG 
CTGCAGATGC TGGAGATGAA CATGGCCATC GCCTTCCCCG CAGCGCCCCT GCTGACCGTC 
ATCCTGGCCC TCGTGGGAAT GGAGGCGATC ATGTCTGAGT TCTTCAATGA CACCACCACG 
GCCTTCTACA TCATCCTCAT CGTGTGGCTG GCTGACCAGT ATGACGCCAT CTGCTGCCAC 
ACCAACACCA GCAAGCGGCA CTGGCTTCGG TTCTTCTACC TGTACCACTT TGCCTTCTAC 
GCTTACCACT ATCGCTTCAA CGGGCAGTAC AGCAGCCTGG CACTCGTCAC CTCCTGGCTC 
TTCATCCAGC ATTCCATGAT CTACTTCTTC CACCACTACG AACTACCTGC CATCCTGCAG 
CAGATCCGTG TCCAGGAGAT GCTGCTGCAG ACACCACCAC TGGGCCCCGG CACCCCCACA 
GCCCTGCCAG ACGACCTGAA CAACAATGGA GGCATCCCAC CTATCACCCC CGACGCTGCC 
AGCCAGTCCC CTGCCCTGGG CCCTGGCTTG CCAGGTGCTG GCGGGGGCCC CGGGCCCATG 
GCTGAGGCAC CTAGCTCCCT GGTGGCCGCA GCGGCCTCAG TGGCTGCAGC AGCCAGTGGT 
GACCTGGGTT GGATGGCAGA GACGGCTGCC ATCATCACAG ACGCCTCCTT CCTGTCTGGC 
CTGAGCGCCT CTCTCCTGGA CCGGCGGCCA GGCAGCCCCC TGAGCCCTAG TGGGGGGCTC 
CTACGGGCCC CCCAGGACAG TGCCCCTGCA AGCAACTCTC CAGGGTCTGA TGCAGTCTCT 
CAGACCACCG GCGGGGGCAG GCCCAACCCT GTGTCTACCG ATCTAGTGGA CACACCGTCG 
GAGGTCGGCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM259 ( XM_024995109.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850877.1 Bos taurus transmembrane protein 259 (TMEM259), transcript variant X5, mRNA.

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CloneID	OBa236636
Clone ID Related Accession (Same CDS sequence)	XM_024995109.1
Accession Version	XM_024995109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	membralin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGC ACGCGGCACC CGGGGCCCCG GGGCCTGGGC CCAACGGCGG TGGTGGCGGT 
GGCGGCGGTG GCGGCGGCGG CCAGGCCCCC GCGCGCGGGC CTCGCACCCC CAACCTCAAC 
CCCAATCCTC TCATCAACGT GCGCGACCGG CTTTTCCACG CGCTCTTCTT CAAGATGGCT 
GTCACCTACT CGCGCCTCTT CCCGCCCGCC TTCCGTCGAC TCTTCGAGTT TTTCGTGTTG 
CTCAAGGCCC TGTTCGTGCT CTTTGTCCTG GCCTACATCC ACATCGTCTT CTCCCGCTCA 
CCCATCAACT GCCTGGAGCA CGTGCGTGAC AAGTGGCCCC GTGAGGGCAT CCTGCGGGTA 
GAGGTGCAGC ACAACTCGAG CCGCGCGCCC GTCTTCCTGC AGTTCTGTGA TGGTGGCCAT 
GGCAGCTTCC CTGGCCTGGC CATGGAGCCA GATGGCCTGG AGCTAGAGGA GGAGGAGGAG 
GAGGAGCTGA CCATGGACAT GTTCGGGAAT AGCTCCATCA AGTTTGAACT GGACATCGAG 
CCCAAGGTGT TCAAGCCACC AGGTGGCCCC AAGGCCTTAA ATGACAGCCA GGAGTTCCCA 
TTCCCTGAGA CGCCCCCAAA AGCCTGGCCA CAAGACGAGT ACATCGTGGA GTACTCGCTG 
GAGTATGGCT TCCTGAGGTT GTCGCAGGCC ACACGCCAGC GGCTCAGCAT CCCCGTAATG 
GTGGTCACGC TGGATCCCAC GCGGGACCAG TGCTTTGGTG ACCGCTTCAG CCGCCTGCTG 
CTGGCTGAGT TCCTGGGCTA TGATGACATC CTCATGTCCA GCGTGAAGGG CCTGGCGGAG 
AACGAGGAGA ACAAAGGCTT CCTGAGGAAT GTGGTGTCCG GGGAGCACTA CCGCTTCGTG 
AGCATGTGGA TGGCCCGCAC GTCCTACCTG GCCGCCTTCG TCATCATGGT CATCTTTACC 
CTGAGCGTGT CCATGTTGCT GCGCTACTCC CACCATCAGA TCTTCGTTTT CATTGGAATG 
GAGGCGATCA TGTCTGAGTT CTTCAATGAC ACCACCACGG CCTTCTACAT CATCCTCATC 
GTGTGGCTGG CTGACCAGTA TGACGCCATC TGCTGCCACA CCAACACCAG CAAGCGGCAC 
TGGCTTCGGT TCTTCTACCT GTACCACTTT GCCTTCTACG CTTACCACTA TCGCTTCAAC 
GGGCAGTACA GCAGCCTGGC ACTCGTCACC TCCTGGCTCT TCATCCAGCA TTCCATGATC 
TACTTCTTCC ACCACTACGA ACTACCTGCC ATCCTGCAGC AGATCCGTGT CCAGGAGATG 
CTGCTGCAGA CACCACCACT GGGCCCCGGC ACCCCCACAG CCCTGCCAGA CGACCTGAAC 
AACAATGGAG GCATCCCACC TATCACCCCC GACGCTGCCA GCCAGTCCCC TGCCCTGGGC 
CCTGGCTTGC CAGGTGCTGG CGGGGGCCCC GGGCCCATGG CTGAGGCACC TAGCTCCCTG 
GTGGCCGCAG CGGCCTCAGT GGCTGCAGCA GCCAGTGGTG ACCTGGGTTG GATGGCAGAG 
ACGGCTGCCA TCATCACAGA CGCCTCCTTC CTGTCTGGCC TGAGCGCCTC TCTCCTGGAC 
CGGCGGCCAG GCAGCCCCCT GAGCCCTAGT GGGGGGCTCC TACGGGCCCC CCAGGACAGT 
GCCCCTGCAA GCAACTCTCC AGGGTCTGAT GCAGTCTCTC AGACCACCGG CGGGGGCAGG 
CCCAACCCTG TGTCTACCGA TCTAGTGGAC ACACCGTCGG AGGTCGGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850877.1
CDS	190..1983
Translation	MSEHAAPGAPGPGPNGGGGGGGGGGGGQAPARGPRTPNLNPNPLINVRDRLFHALFFKMAVTYSRLFPPAFRRLFEFFVLLKALFVLFVLAYIHIVFSRSPINCLEHVRDKWPREGILRVEVQHNSSRAPVFLQFCDGGHGSFPGLAMEPDGLELEEEEEEELTMDMFGNSSIKFELDIEPKVFKPPGGPKALNDSQEFPFPETPPKAWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLAEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIGMEAIMSEFFNDTTTAFYIILIVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRVQEMLLQTPPLGPGTPTALPDDLNNNGGIPPITPDAASQSPALGPGLPGAGGGPGPMAEAPSSLVAAAASVAAAASGDLGWMAETAAIITDASFLSGLSASLLDRRPGSPLSPSGGLLRAPQDSAPASNSPGSDAVSQTTGGGRPNPVSTDLVDTPSEVGS

Target ORF information:

RefSeq Version	XM_024995109.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 259 (TMEM259), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995109.1

ORF Insert Sequence:

ATGTCGGAGC ACGCGGCACC CGGGGCCCCG GGGCCTGGGC CCAACGGCGG TGGTGGCGGT 
GGCGGCGGTG GCGGCGGCGG CCAGGCCCCC GCGCGCGGGC CTCGCACCCC CAACCTCAAC 
CCCAATCCTC TCATCAACGT GCGCGACCGG CTTTTCCACG CGCTCTTCTT CAAGATGGCT 
GTCACCTACT CGCGCCTCTT CCCGCCCGCC TTCCGTCGAC TCTTCGAGTT TTTCGTGTTG 
CTCAAGGCCC TGTTCGTGCT CTTTGTCCTG GCCTACATCC ACATCGTCTT CTCCCGCTCA 
CCCATCAACT GCCTGGAGCA CGTGCGTGAC AAGTGGCCCC GTGAGGGCAT CCTGCGGGTA 
GAGGTGCAGC ACAACTCGAG CCGCGCGCCC GTCTTCCTGC AGTTCTGTGA TGGTGGCCAT 
GGCAGCTTCC CTGGCCTGGC CATGGAGCCA GATGGCCTGG AGCTAGAGGA GGAGGAGGAG 
GAGGAGCTGA CCATGGACAT GTTCGGGAAT AGCTCCATCA AGTTTGAACT GGACATCGAG 
CCCAAGGTGT TCAAGCCACC AGGTGGCCCC AAGGCCTTAA ATGACAGCCA GGAGTTCCCA 
TTCCCTGAGA CGCCCCCAAA AGCCTGGCCA CAAGACGAGT ACATCGTGGA GTACTCGCTG 
GAGTATGGCT TCCTGAGGTT GTCGCAGGCC ACACGCCAGC GGCTCAGCAT CCCCGTAATG 
GTGGTCACGC TGGATCCCAC GCGGGACCAG TGCTTTGGTG ACCGCTTCAG CCGCCTGCTG 
CTGGCTGAGT TCCTGGGCTA TGATGACATC CTCATGTCCA GCGTGAAGGG CCTGGCGGAG 
AACGAGGAGA ACAAAGGCTT CCTGAGGAAT GTGGTGTCCG GGGAGCACTA CCGCTTCGTG 
AGCATGTGGA TGGCCCGCAC GTCCTACCTG GCCGCCTTCG TCATCATGGT CATCTTTACC 
CTGAGCGTGT CCATGTTGCT GCGCTACTCC CACCATCAGA TCTTCGTTTT CATTGGAATG 
GAGGCGATCA TGTCTGAGTT CTTCAATGAC ACCACCACGG CCTTCTACAT CATCCTCATC 
GTGTGGCTGG CTGACCAGTA TGACGCCATC TGCTGCCACA CCAACACCAG CAAGCGGCAC 
TGGCTTCGGT TCTTCTACCT GTACCACTTT GCCTTCTACG CTTACCACTA TCGCTTCAAC 
GGGCAGTACA GCAGCCTGGC ACTCGTCACC TCCTGGCTCT TCATCCAGCA TTCCATGATC 
TACTTCTTCC ACCACTACGA ACTACCTGCC ATCCTGCAGC AGATCCGTGT CCAGGAGATG 
CTGCTGCAGA CACCACCACT GGGCCCCGGC ACCCCCACAG CCCTGCCAGA CGACCTGAAC 
AACAATGGAG GCATCCCACC TATCACCCCC GACGCTGCCA GCCAGTCCCC TGCCCTGGGC 
CCTGGCTTGC CAGGTGCTGG CGGGGGCCCC GGGCCCATGG CTGAGGCACC TAGCTCCCTG 
GTGGCCGCAG CGGCCTCAGT GGCTGCAGCA GCCAGTGGTG ACCTGGGTTG GATGGCAGAG 
ACGGCTGCCA TCATCACAGA CGCCTCCTTC CTGTCTGGCC TGAGCGCCTC TCTCCTGGAC 
CGGCGGCCAG GCAGCCCCCT GAGCCCTAGT GGGGGGCTCC TACGGGCCCC CCAGGACAGT 
GCCCCTGCAA GCAACTCTCC AGGGTCTGAT GCAGTCTCTC AGACCACCGG CGGGGGCAGG 
CCCAACCCTG TGTCTACCGA TCTAGTGGAC ACACCGTCGG AGGTCGGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM259 cDNA ORF clone, Bos taurus(cattle)

Summary

Reference Sequences (RefSeq)

Pathways from BioSystems

Homologies

Gene Ontology

Reference Sequences (Refseq)

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PubMed