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tmem259 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Summary

Gene Symbol tmem259
Entrez Gene ID 779707
Full Name transmembrane protein 259
Synonyms c19orf6
General protein information
Preferred Names

transmembrane protein 259

Names

membralin
Gene Type protein-coding
Organism Xenopus tropicalis(tropical clawed frog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_018094323.1 XP_017949812.1 membralin isoform X1
XM_012965024.2 XP_012820478.1 membralin isoform X3
NM_001078786.1 NP_001072254.1 membralin
XM_012965019.2 XP_012820473.1 membralin isoform X2
XM_012965019.3 XP_012820473.1 membralin isoform X1

Pathways from BioSystems


Homologies


Homo sapiens (human) TMEM259 NP_001028198.1
Pan troglodytes (chimpanzee) C19H19orf6 XP_003954544.1
Xenopus tropicalis (tropical clawed frog) tmem259 NP_001072254.1
Bos taurus (cattle) TMEM259 XP_002689215.2
Danio rerio (zebrafish) tmem259 XP_700127.3
Canis lupus familiaris (dog) C20H19orf6 XP_533963.2
Gallus gallus (chicken) C19ORF6 XP_418222.2
Mus musculus (house mouse) Tmem259 NP_001003949.2
Rattus norvegicus (Norway rat) Mbrl NP_001094356.1

Gene Ontology


Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tmem259 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tmem259 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa01257 NM_001078786.1
Latest version!		 Xenopus tropicalis transmembrane protein 259 (tmem259), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
OXa29108 XM_012965019.2
Latest version!		 Xenopus tropicalis transmembrane protein 259 (tmem259), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00
OXa29109 XM_012965024.2
Latest version!		 Xenopus tropicalis transmembrane protein 259 (tmem259), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00
OXa41861 XM_018094323.1
Latest version!		 Xenopus tropicalis transmembrane protein 259 (tmem259), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $258.30
$369.00
OXa48004 XM_012965019.3
Latest version!		 Xenopus tropicalis transmembrane protein 259 (tmem259), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OXa01257
Clone ID Related Accession (Same CDS sequence) NM_001078786.1
Accession Version NM_001078786.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1884bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-21
Organism Xenopus tropicalis(tropical clawed frog)
Product membralin
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC121219.1. ##Evidence-Data-START## Transcript exon combination :: BC121219.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGTCTGAGA ACCAGGGTAA TATTCCTCTC AATAACGCTA ATGGCAACCG TGTTCGAACT 
CCTAACCTGA ATCAGAATCC CTTGATTAAT GTTCGAGATC GTCTCTTCCA TGCATTGTTC 
TTCAAGATGG CTGTGACCTA CGCAAGACTG TTCCCCCCTT CCTTTCGCAG AATATTTGAG 
TTCTTTGTAC TTCTGAAGGC CCTCCTGGTA CTGTTTATCC TTGCATATAT CCACATTGCA 
TTCTCCCGTT CCCCAATCAA CTGCTTGGAG CATGTTAGAG AGAAATGGCC TCGGGATGGA 
ATCTTGCGAG TGGAGATTCA GCTGAACTCA AGCCGAGCAC CCATCTTTTT ACAATTTTAT 
GACAGTGACA CATTTCCAGG TTTGGTAAAG GAACCATCTG GGGAAGATGA GGAGGAGGAG 
GAAGAGATGA GCGTGGAAAT GTACCACAAT AGCTCCATCA AGTTTGAGCT GGATATTGAG 
CCCAAAGTAT TCCTGAAGCC ATCTCTAGAG AGTGTTACTC AGGCCTCTGA AAACCAGAGC 
CAGACACTGT CCTTCAGTGA TGCTGCTAGC AAAGTGTGGC CACAAGAAGA GTACATCGTG 
GAATATTCCT TAGAATATGG CTTCCTGCGG CTGTCTCAGA GCACAAGGCA GAGGCTGAAT 
ATCCCTGTTA TGGTGGTTAC ACTAGACCCA ACTCGAGATC TGTGCTTTGG AGACAGATTC 
AGTCGATTTT TGCTGGATGA GTTTCTGGGA TATGATGATA TTCTTATGTC TAGTGTAAAG 
GCCCTGGCAG AAAATGAGGA AAATAAAGGA TTCCTAAGGA ATGTGGTTTC TGGGGAACAT 
TATCGATTTG TCAGTATGTG GATGGCTCGT ACTTCTTACC TTGCTGCCTT CGTCATCATG 
GTTATATTTA CACTCTCAGT CTCAATGCTT CTGCGGTATT CCCATCATCA AATCTTTGTA 
TTTATTGTGG ACTTGCTACA GATGTTAGAG ATGAATATGA GTATCGCGTT TCCTGCTGCA 
CCATTGCTCA CTGTGATCCT GGCTCTTGTT GGGATGGAAG CCATCATGTC AGAATTTTTC 
AATGATACCA CCACTGCTTT CTATATAATC CTCATTGTGT GGTTAGCTGA TCAGTATGAT 
GCCATATGCT GCCATACAAA CACCAGCAAG AGACACTGGC TCAGGTTCTT CTATCTTTAT 
CACTTTGCAT TTTACGCTTA CCACTATCGA TTTAATGGCC AATACAGCAG CCTTGCCCTT 
GTTACATCGT GGTTATTCAT TCAGCACTCA ATGATCTATT TCTTCCATCA CTACGAGCTT 
CCTGCCATCC TGCAACAGAT AAGAATCCAA GAGATGCTTC TTCAAAACCA GCAAGCAGGG 
CAAAATCAGA CCAGCCTACA GGACAATCTC AACAATAATA CTGCATCAGA GCCATCAGGG 
GCAACACCTC CTCAGGCTCC CGGAGATCCC AGCCACCCAC CAGTAGGGAG TGACTACAGC 
ACTACCGAAG GGGCTTTAAG TGACGTGTTG CTTGAAGAGG TGACAGGAAC CTCACAGCAA 
GTGGTAACTG GGATACTACC TGCCGCAGAC GAAAGAGCCC AAGAAGGAGT TTGCGTCGGT 
ACTCATACAC CAGAAATTGC AGCAGTAACA CAAACTGGAG ATCCTCCTCC TGTTCTGAAT 
GTTTCCCACA ATTCCTGCTT AACAGATCAG ACTGAGCTTC AGGGGGGCAT GGTATCCCCC 
ATAATAGACC TTAGTTCTCT GAGATCCTTA GCAATCAATT TAGAGTCAGA GCAGAGCAGT 
CACACTATAG CTGCTTCTGC ATTACAGTGT GTAGAACCCA CTTCCCAGAT AAATGATAAA 
GAGCACCCTG CAGTGGACTT GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001072254.1
CDS164..2047
Translation

Target ORF information:

RefSeq Version NM_001078786.1
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis transmembrane protein 259 (tmem259), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001078786.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGTCTGAGA ACCAGGGTAA TATTCCTCTC AATAACGCTA ATGGCAACCG TGTTCGAACT 
CCTAACCTGA ATCAGAATCC CTTGATTAAT GTTCGAGATC GTCTCTTCCA TGCATTGTTC 
TTCAAGATGG CTGTGACCTA CGCAAGACTG TTCCCCCCTT CCTTTCGCAG AATATTTGAG 
TTCTTTGTAC TTCTGAAGGC CCTCCTGGTA CTGTTTATCC TTGCATATAT CCACATTGCA 
TTCTCCCGTT CCCCAATCAA CTGCTTGGAG CATGTTAGAG AGAAATGGCC TCGGGATGGA 
ATCTTGCGAG TGGAGATTCA GCTGAACTCA AGCCGAGCAC CCATCTTTTT ACAATTTTAT 
GACAGTGACA CATTTCCAGG TTTGGTAAAG GAACCATCTG GGGAAGATGA GGAGGAGGAG 
GAAGAGATGA GCGTGGAAAT GTACCACAAT AGCTCCATCA AGTTTGAGCT GGATATTGAG 
CCCAAAGTAT TCCTGAAGCC ATCTCTAGAG AGTGTTACTC AGGCCTCTGA AAACCAGAGC 
CAGACACTGT CCTTCAGTGA TGCTGCTAGC AAAGTGTGGC CACAAGAAGA GTACATCGTG 
GAATATTCCT TAGAATATGG CTTCCTGCGG CTGTCTCAGA GCACAAGGCA GAGGCTGAAT 
ATCCCTGTTA TGGTGGTTAC ACTAGACCCA ACTCGAGATC TGTGCTTTGG AGACAGATTC 
AGTCGATTTT TGCTGGATGA GTTTCTGGGA TATGATGATA TTCTTATGTC TAGTGTAAAG 
GCCCTGGCAG AAAATGAGGA AAATAAAGGA TTCCTAAGGA ATGTGGTTTC TGGGGAACAT 
TATCGATTTG TCAGTATGTG GATGGCTCGT ACTTCTTACC TTGCTGCCTT CGTCATCATG 
GTTATATTTA CACTCTCAGT CTCAATGCTT CTGCGGTATT CCCATCATCA AATCTTTGTA 
TTTATTGTGG ACTTGCTACA GATGTTAGAG ATGAATATGA GTATCGCGTT TCCTGCTGCA 
CCATTGCTCA CTGTGATCCT GGCTCTTGTT GGGATGGAAG CCATCATGTC AGAATTTTTC 
AATGATACCA CCACTGCTTT CTATATAATC CTCATTGTGT GGTTAGCTGA TCAGTATGAT 
GCCATATGCT GCCATACAAA CACCAGCAAG AGACACTGGC TCAGGTTCTT CTATCTTTAT 
CACTTTGCAT TTTACGCTTA CCACTATCGA TTTAATGGCC AATACAGCAG CCTTGCCCTT 
GTTACATCGT GGTTATTCAT TCAGCACTCA ATGATCTATT TCTTCCATCA CTACGAGCTT 
CCTGCCATCC TGCAACAGAT AAGAATCCAA GAGATGCTTC TTCAAAACCA GCAAGCAGGG 
CAAAATCAGA CCAGCCTACA GGACAATCTC AACAATAATA CTGCATCAGA GCCATCAGGG 
GCAACACCTC CTCAGGCTCC CGGAGATCCC AGCCACCCAC CAGTAGGGAG TGACTACAGC 
ACTACCGAAG GGGCTTTAAG TGACGTGTTG CTTGAAGAGG TGACAGGAAC CTCACAGCAA 
GTGGTAACTG GGATACTACC TGCCGCAGAC GAAAGAGCCC AAGAAGGAGT TTGCGTCGGT 
ACTCATACAC CAGAAATTGC AGCAGTAACA CAAACTGGAG ATCCTCCTCC TGTTCTGAAT 
GTTTCCCACA ATTCCTGCTT AACAGATCAG ACTGAGCTTC AGGGGGGCAT GGTATCCCCC 
ATAATAGACC TTAGTTCTCT GAGATCCTTA GCAATCAATT TAGAGTCAGA GCAGAGCAGT 
CACACTATAG CTGCTTCTGC ATTACAGTGT GTAGAACCCA CTTCCCAGAT AAATGATAAA 
GAGCACCCTG CAGTGGACTT GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa29108
Clone ID Related Accession (Same CDS sequence) XM_012965019.2
Accession Version XM_012965019.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-09-07
Organism Xenopus tropicalis(tropical clawed frog)
Product membralin isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965019.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTCTGAGA ACCAGGGTAA TATTCCTCTC AATAACGCTA ATGGCAACCG TGTTCGAACT 
CCTAACCTGA ATCAGAATCC CTTGATTAAT GTTCGAGATC GTCTCTTCCA TGCATTGTTC 
TTCAAGATGG CTGTGACCTA CGCAAGACTG TTCCCCCCTT CCTTTCGCAG AATATTTGAG 
TTCTTTGTAC TTCTGAAGGC CCTCCTGGTA CTGTTTATCC TTGCATATAT CCACATTGCA 
TTCTCCCGTT CACCAATCAA CTGCTTGGAG CATGTTAGAG AGAAATGGCC TCGGGATGGA 
ATCTTGCGAG TGGAGATTCA GCTGAACTCA AGCCGAGCAC CCATCTTTTT ACAATTTTAT 
GACAGTGACA CATTTCCAGG TTTGGTAAAG GAACCATCTG GGGAAGATGA GGAGGAGGAG 
GAAGAGATGA GCGTGGAAAT GTACCACAAT AGCTCCATCA AGTTTGAGCT GGATATTGAG 
CCCAAAGTAT TCCTGAAGCC ATCTCTAGAG AGTGTTACTC AGGCCTCTGA AAACCAGAGC 
CAGACACTGT CCTTCAGTGA TGCTGCTAGC AAAGTGTGGC CACAAGAAGA GTACATCGTG 
GAATATTCCT TAGAATATGG CTTCCTGCGG CTGTCACAGA GCACAAGGCA GAGGCTGAAT 
ATCCCTGTTA TGGTGGTTAC ACTAGACCCA ACTCGAGATC TGTGCTTTGG AGACAGATTC 
AGTCGATTTT TGCTGGATGA GTTTCTGGGA TATGATGATA TTCTTATGTC TAGTGTAAAG 
GCCCTGGCAG AAAATGAGGA AAATAAAGGA TTTCTAAGGA ATGTGGTTTC TGGGGAACAT 
TATCGATTTG TCAGTATGTG GATGGCTCGT ACTTCTTACC TTGCTGCCTT CGTCATCATG 
GTTATATTTA CACTCTCAGT CTCAATGCTT CTGCGGTATT CCCATCATCA AATCTTTGTA 
TTTATTGTGG ACTTGCTACA GATGTTAGAG ATGAATATGA GTATCGCGTT TCCTGCTGCA 
CCGTTGCTCA CTGTGATCCT GGCTCTTGTT GGGATGGAAG CCATCATGTC AGAATTTTTC 
AATGATACCA CCACTGCTTT CTATATAATC CTCATTGTGT GGCTAGCTGA TCAGTATGAT 
GCCATATGCT GCCATACAAA CACCAGCAAG AGACACTGGC TCAGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_012820473.1
CDS241..1428
Translation

Target ORF information:

RefSeq Version XM_012965019.2
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis transmembrane protein 259 (tmem259), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_012965019.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTCTGAGA ACCAGGGTAA TATTCCTCTC AATAACGCTA ATGGCAACCG TGTTCGAACT 
CCTAACCTGA ATCAGAATCC CTTGATTAAT GTTCGAGATC GTCTCTTCCA TGCATTGTTC 
TTCAAGATGG CTGTGACCTA CGCAAGACTG TTCCCCCCTT CCTTTCGCAG AATATTTGAG 
TTCTTTGTAC TTCTGAAGGC CCTCCTGGTA CTGTTTATCC TTGCATATAT CCACATTGCA 
TTCTCCCGTT CACCAATCAA CTGCTTGGAG CATGTTAGAG AGAAATGGCC TCGGGATGGA 
ATCTTGCGAG TGGAGATTCA GCTGAACTCA AGCCGAGCAC CCATCTTTTT ACAATTTTAT 
GACAGTGACA CATTTCCAGG TTTGGTAAAG GAACCATCTG GGGAAGATGA GGAGGAGGAG 
GAAGAGATGA GCGTGGAAAT GTACCACAAT AGCTCCATCA AGTTTGAGCT GGATATTGAG 
CCCAAAGTAT TCCTGAAGCC ATCTCTAGAG AGTGTTACTC AGGCCTCTGA AAACCAGAGC 
CAGACACTGT CCTTCAGTGA TGCTGCTAGC AAAGTGTGGC CACAAGAAGA GTACATCGTG 
GAATATTCCT TAGAATATGG CTTCCTGCGG CTGTCACAGA GCACAAGGCA GAGGCTGAAT 
ATCCCTGTTA TGGTGGTTAC ACTAGACCCA ACTCGAGATC TGTGCTTTGG AGACAGATTC 
AGTCGATTTT TGCTGGATGA GTTTCTGGGA TATGATGATA TTCTTATGTC TAGTGTAAAG 
GCCCTGGCAG AAAATGAGGA AAATAAAGGA TTTCTAAGGA ATGTGGTTTC TGGGGAACAT 
TATCGATTTG TCAGTATGTG GATGGCTCGT ACTTCTTACC TTGCTGCCTT CGTCATCATG 
GTTATATTTA CACTCTCAGT CTCAATGCTT CTGCGGTATT CCCATCATCA AATCTTTGTA 
TTTATTGTGG ACTTGCTACA GATGTTAGAG ATGAATATGA GTATCGCGTT TCCTGCTGCA 
CCGTTGCTCA CTGTGATCCT GGCTCTTGTT GGGATGGAAG CCATCATGTC AGAATTTTTC 
AATGATACCA CCACTGCTTT CTATATAATC CTCATTGTGT GGCTAGCTGA TCAGTATGAT 
GCCATATGCT GCCATACAAA CACCAGCAAG AGACACTGGC TCAGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa29109
Clone ID Related Accession (Same CDS sequence) XM_012965024.2
Accession Version XM_012965024.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1161bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-09-07
Organism Xenopus tropicalis(tropical clawed frog)
Product membralin isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965024.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTCTGAGA ACCAGGGTAA TATTCCTCTC AATAACGCTA ATGGCAACCG TGTTCGAACT 
CCTAACCTGA ATCAGAATCC CTTGATTAAT GTTCGAGATC GTCTCTTCCA TGCATTGTTC 
TTCAAGATGG CTGTGACCTA CGCAAGACTG TTCCCCCCTT CCTTTCGCAG AATATTTGAG 
TTCTTTGTAC TTCTGAAGGC CCTCCTGGTA CTGTTTATCC TTGCATATAT CCACATTGCA 
TTCTCCCGTT CACCAATCAA CTGCTTGGAG CATGTTAGAG AGAAATGGCC TCGGGATGGA 
ATCTTGCGAG TGGAGATTCA GCTGAACTCA AGCCGAGCAC CCATCTTTTT ACAATTTTAT 
GACAGTGACA CATTTCCAGG TTTGGTAAAG GAACCATCTG GGGAAGATGA GGAGGAGGAG 
GAAGAGATGA GCGTGGAAAT GTACCACAAT AGCTCCATCA AGTTTGAGCT GGATATTGAG 
CCCAAAGTAT TCCTGAAGCC ATCTCTAGAG AGTGTTACTC AGGCCTCTGA AAACCAGAGC 
CAGACACTGT CCTTCAGTGA TGCTGCTAGC AAAGTGTGGC CACAAGAAGA GTACATCGTG 
GAATATTCCT TAGAATATGG CTTCCTGCGG CTGTCACAGA GCACAAGGCA GAGGCTGAAT 
ATCCCTGTTA TGGTGGTTAC ACTAGACCCA ACTCGAGATC TGTGCTTTGG AGACAGATTC 
AGTCGATTTT TGCTGGATGA GTTTCTGGGA TATGATGATA TTCTTATGTC TAGTGTAAAG 
GCCCTGGCAG AAAATGAGGA AAATAAAGGA TTTCTAAGGA ATGTGGTTTC TGGGGAACAT 
TATCGATTTG TCAGTATGTG GATGGCTCGT ACTTCTTACC TTGCTGCCTT CGTCATCATG 
GTTATATTTA CACTCTCAGT CTCAATGCTT CTGCGGTATT CCCATCATCA AATCTTTGTA 
TTTATTGTGG ACTTGCTACA GATGTTAGAG ATGAATATGA GTATCGCGTT TCCTGCTGCA 
CCGTTGCTCA CTGTGATCCT GGCTCTTGTT GGTTCTTCTA TCTTTATCAC TTTGCATTTT 
ACGCTTACCA CTATCGATTT AATGGCCAAT ACAGCAGCCT TGCCCTTGTT ACATCGTGGT 
TATTCATTCA GCACTCAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_012820478.1
CDS242..1402
Translation

Target ORF information:

RefSeq Version XM_012965024.2
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis transmembrane protein 259 (tmem259), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_012965024.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTCTGAGA ACCAGGGTAA TATTCCTCTC AATAACGCTA ATGGCAACCG TGTTCGAACT 
CCTAACCTGA ATCAGAATCC CTTGATTAAT GTTCGAGATC GTCTCTTCCA TGCATTGTTC 
TTCAAGATGG CTGTGACCTA CGCAAGACTG TTCCCCCCTT CCTTTCGCAG AATATTTGAG 
TTCTTTGTAC TTCTGAAGGC CCTCCTGGTA CTGTTTATCC TTGCATATAT CCACATTGCA 
TTCTCCCGTT CACCAATCAA CTGCTTGGAG CATGTTAGAG AGAAATGGCC TCGGGATGGA 
ATCTTGCGAG TGGAGATTCA GCTGAACTCA AGCCGAGCAC CCATCTTTTT ACAATTTTAT 
GACAGTGACA CATTTCCAGG TTTGGTAAAG GAACCATCTG GGGAAGATGA GGAGGAGGAG 
GAAGAGATGA GCGTGGAAAT GTACCACAAT AGCTCCATCA AGTTTGAGCT GGATATTGAG 
CCCAAAGTAT TCCTGAAGCC ATCTCTAGAG AGTGTTACTC AGGCCTCTGA AAACCAGAGC 
CAGACACTGT CCTTCAGTGA TGCTGCTAGC AAAGTGTGGC CACAAGAAGA GTACATCGTG 
GAATATTCCT TAGAATATGG CTTCCTGCGG CTGTCACAGA GCACAAGGCA GAGGCTGAAT 
ATCCCTGTTA TGGTGGTTAC ACTAGACCCA ACTCGAGATC TGTGCTTTGG AGACAGATTC 
AGTCGATTTT TGCTGGATGA GTTTCTGGGA TATGATGATA TTCTTATGTC TAGTGTAAAG 
GCCCTGGCAG AAAATGAGGA AAATAAAGGA TTTCTAAGGA ATGTGGTTTC TGGGGAACAT 
TATCGATTTG TCAGTATGTG GATGGCTCGT ACTTCTTACC TTGCTGCCTT CGTCATCATG 
GTTATATTTA CACTCTCAGT CTCAATGCTT CTGCGGTATT CCCATCATCA AATCTTTGTA 
TTTATTGTGG ACTTGCTACA GATGTTAGAG ATGAATATGA GTATCGCGTT TCCTGCTGCA 
CCGTTGCTCA CTGTGATCCT GGCTCTTGTT GGTTCTTCTA TCTTTATCAC TTTGCATTTT 
ACGCTTACCA CTATCGATTT AATGGCCAAT ACAGCAGCCT TGCCCTTGTT ACATCGTGGT 
TATTCATTCA GCACTCAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa41861
Clone ID Related Accession (Same CDS sequence) XM_018094323.1
Accession Version XM_018094323.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1215bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-09-07
Organism Xenopus tropicalis(tropical clawed frog)
Product membralin isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGGTTA CACTAGACCC AACTCGAGAT CTGTGCTTTG GAGACAGATT CAGTCGATTT 
TTGCTGGATG AGTTTCTGGG ATATGATGAT ATTCTTATGT CTAGTGTAAA GGCCCTGGCA 
GAAAATGAGG AAAATAAAGG ATTTCTAAGG AATGTGGTTT CTGGGGAACA TTATCGATTT 
GTCAGTATGT GGATGGCTCG TACTTCTTAC CTTGCTGCCT TCGTCATCAT GGTTATATTT 
ACACTCTCAG TCTCAATGCT TCTGCGGTAT TCCCATCATC AAATCTTTGT ATTTATTGTG 
GACTTGCTAC AGATGTTAGA GATGAATATG AGTATCGCGT TTCCTGCTGC ACCGTTGCTC 
ACTGTGATCC TGGCTCTTGT TGGGATGGAA GCCATCATGT CAGAATTTTT CAATGATACC 
ACCACTGCTT TCTATATAAT CCTCATTGTG TGGCTAGCTG ATCAGTATGA TGCCATATGC 
TGCCATACAA ACACCAGCAA GAGACACTGG CTCAGGTTCT TCTATCTTTA TCACTTTGCA 
TTTTACGCTT ACCACTATCG ATTTAATGGC CAATACAGCA GCCTTGCCCT TGTTACATCG 
TGGTTATTCA TTCAGCACTC AATGATCTAT TTCTTCCATC ACTACGAGCT TCCTGCCATC 
CTGCAACAGA TAAGAATCCA AGAGATGCTT CTTCAAAACC AGCAAGCAGG GCAAAATCAG 
ACCAGCCTAC AGGACAATCT CAACAATAAT ACTGCATCAG AGCCATCAGG GGCAACACCT 
CCTCAGGCTC CCGGAGATCC CAGCCACCCA CCAGTAGGGA GTGACTACAG CACTACCGAA 
GGGGCTTTAA GTGACGTGTT GCTTGAAGAG GTGACAGGAA CCTCACAGCA AGTGGTAACT 
GGGATACTAC CTGCCGCAGA CGAAAGAGCC CAAGAAGGAG TTTGCGTCGG TACTCATACA 
CCAGAAATTG CAGCAGTAAC ACAAACTGGA GATCCTCCTC CTGTTCTGAA TGTTTCCCAC 
AATTCCTGCT TAACAGATCA GACTGAGCTT CAGGGGGGCA TGGTATCCCC CATAATAGAC 
CTTAGTTCTC TGAGATCCTT AGCAATCAAT TTAGAGTCAG AGCAGAGCAG TCACACTATA 
GCTGCTTCTG CATTACAGTG TGTAGAACCC ACTTCCCAGA TAAATGATAA AGAGCACCCT 
GCAGTGGACT TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017949812.1
CDS185..1399
Translation

Target ORF information:

RefSeq Version XM_018094323.1
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis transmembrane protein 259 (tmem259), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_018094323.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGGTTA CACTAGACCC AACTCGAGAT CTGTGCTTTG GAGACAGATT CAGTCGATTT 
TTGCTGGATG AGTTTCTGGG ATATGATGAT ATTCTTATGT CTAGTGTAAA GGCCCTGGCA 
GAAAATGAGG AAAATAAAGG ATTTCTAAGG AATGTGGTTT CTGGGGAACA TTATCGATTT 
GTCAGTATGT GGATGGCTCG TACTTCTTAC CTTGCTGCCT TCGTCATCAT GGTTATATTT 
ACACTCTCAG TCTCAATGCT TCTGCGGTAT TCCCATCATC AAATCTTTGT ATTTATTGTG 
GACTTGCTAC AGATGTTAGA GATGAATATG AGTATCGCGT TTCCTGCTGC ACCGTTGCTC 
ACTGTGATCC TGGCTCTTGT TGGGATGGAA GCCATCATGT CAGAATTTTT CAATGATACC 
ACCACTGCTT TCTATATAAT CCTCATTGTG TGGCTAGCTG ATCAGTATGA TGCCATATGC 
TGCCATACAA ACACCAGCAA GAGACACTGG CTCAGGTTCT TCTATCTTTA TCACTTTGCA 
TTTTACGCTT ACCACTATCG ATTTAATGGC CAATACAGCA GCCTTGCCCT TGTTACATCG 
TGGTTATTCA TTCAGCACTC AATGATCTAT TTCTTCCATC ACTACGAGCT TCCTGCCATC 
CTGCAACAGA TAAGAATCCA AGAGATGCTT CTTCAAAACC AGCAAGCAGG GCAAAATCAG 
ACCAGCCTAC AGGACAATCT CAACAATAAT ACTGCATCAG AGCCATCAGG GGCAACACCT 
CCTCAGGCTC CCGGAGATCC CAGCCACCCA CCAGTAGGGA GTGACTACAG CACTACCGAA 
GGGGCTTTAA GTGACGTGTT GCTTGAAGAG GTGACAGGAA CCTCACAGCA AGTGGTAACT 
GGGATACTAC CTGCCGCAGA CGAAAGAGCC CAAGAAGGAG TTTGCGTCGG TACTCATACA 
CCAGAAATTG CAGCAGTAAC ACAAACTGGA GATCCTCCTC CTGTTCTGAA TGTTTCCCAC 
AATTCCTGCT TAACAGATCA GACTGAGCTT CAGGGGGGCA TGGTATCCCC CATAATAGAC 
CTTAGTTCTC TGAGATCCTT AGCAATCAAT TTAGAGTCAG AGCAGAGCAG TCACACTATA 
GCTGCTTCTG CATTACAGTG TGTAGAACCC ACTTCCCAGA TAAATGATAA AGAGCACCCT 
GCAGTGGACT TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa48004
Clone ID Related Accession (Same CDS sequence) XM_012965019.3
Accession Version XM_012965019.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-17
Organism Xenopus tropicalis(tropical clawed frog)
Product membralin isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965019.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTCTGAGA ACCAGGGTAA TATTCCTCTC AATAACGCTA ATGGCAACCG TGTTCGAACT 
CCTAACCTGA ATCAGAATCC CTTGATTAAT GTTCGAGATC GTCTCTTCCA TGCATTGTTC 
TTCAAGATGG CTGTGACCTA CGCAAGACTG TTCCCCCCTT CCTTTCGCAG AATATTTGAG 
TTCTTTGTAC TTCTGAAGGC CCTCCTGGTA CTGTTTATCC TTGCATATAT CCACATTGCA 
TTCTCCCGTT CCCCAATCAA CTGCTTGGAG CATGTTAGAG AGAAATGGCC TCGGGATGGA 
ATCTTGCGAG TGGAGATTCA GCTGAACTCA AGCCGAGCAC CCATCTTTTT ACAATTTTAT 
GACAGTGACA CATTTCCAGG TTTGGTAAAG GAACCATCTG GGGAAGATGA GGAGGAGGAG 
GAAGAGATGA GCGTGGAAAT GTACCACAAT AGCTCCATCA AGTTTGAGCT GGATATTGAG 
CCCAAAGTAT TCCTGAAGCC ATCTCTAGAG AGTGTTACTC AGGCCTCTGA AAACCAGAGC 
CAGACACTGT CCTTCAGTGA TGCTGCTAGC AAAGTGTGGC CACAAGAAGA GTACATCGTG 
GAATATTCCT TAGAATATGG CTTCCTGCGG CTGTCTCAGA GCACAAGGCA GAGGCTGAAT 
ATCCCTGTTA TGGTGGTTAC ACTAGACCCA ACTCGAGATC TGTGCTTTGG AGACAGATTC 
AGTCGATTTT TGCTGGATGA GTTTCTGGGA TATGATGATA TTCTTATGTC TAGTGTAAAG 
GCCCTGGCAG AAAATGAGGA AAATAAAGGA TTCCTAAGGA ATGTGGTTTC TGGGGAACAT 
TATCGATTTG TCAGTATGTG GATGGCTCGT ACTTCTTACC TTGCTGCCTT CGTCATCATG 
GTTATATTTA CACTCTCAGT CTCAATGCTT CTGCGGTATT CCCATCATCA AATCTTTGTA 
TTTATTGTGG ACTTGCTACA GATGTTAGAG ATGAATATGA GTATCGCGTT TCCTGCTGCA 
CCATTGCTCA CTGTGATCCT GGCTCTTGTT GGGATGGAAG CCATCATGTC AGAATTTTTC 
AATGATACCA CCACTGCTTT CTATATAATC CTCATTGTGT GGTTAGCTGA TCAGTATGAT 
GCCATATGCT GCCATACAAA CACCAGCAAG AGACACTGGC TCAGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_012820473.1
CDS227..1414
Translation

Target ORF information:

RefSeq Version XM_012965019.3
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis transmembrane protein 259 (tmem259), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_012965019.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTCTGAGA ACCAGGGTAA TATTCCTCTC AATAACGCTA ATGGCAACCG TGTTCGAACT 
CCTAACCTGA ATCAGAATCC CTTGATTAAT GTTCGAGATC GTCTCTTCCA TGCATTGTTC 
TTCAAGATGG CTGTGACCTA CGCAAGACTG TTCCCCCCTT CCTTTCGCAG AATATTTGAG 
TTCTTTGTAC TTCTGAAGGC CCTCCTGGTA CTGTTTATCC TTGCATATAT CCACATTGCA 
TTCTCCCGTT CCCCAATCAA CTGCTTGGAG CATGTTAGAG AGAAATGGCC TCGGGATGGA 
ATCTTGCGAG TGGAGATTCA GCTGAACTCA AGCCGAGCAC CCATCTTTTT ACAATTTTAT 
GACAGTGACA CATTTCCAGG TTTGGTAAAG GAACCATCTG GGGAAGATGA GGAGGAGGAG 
GAAGAGATGA GCGTGGAAAT GTACCACAAT AGCTCCATCA AGTTTGAGCT GGATATTGAG 
CCCAAAGTAT TCCTGAAGCC ATCTCTAGAG AGTGTTACTC AGGCCTCTGA AAACCAGAGC 
CAGACACTGT CCTTCAGTGA TGCTGCTAGC AAAGTGTGGC CACAAGAAGA GTACATCGTG 
GAATATTCCT TAGAATATGG CTTCCTGCGG CTGTCTCAGA GCACAAGGCA GAGGCTGAAT 
ATCCCTGTTA TGGTGGTTAC ACTAGACCCA ACTCGAGATC TGTGCTTTGG AGACAGATTC 
AGTCGATTTT TGCTGGATGA GTTTCTGGGA TATGATGATA TTCTTATGTC TAGTGTAAAG 
GCCCTGGCAG AAAATGAGGA AAATAAAGGA TTCCTAAGGA ATGTGGTTTC TGGGGAACAT 
TATCGATTTG TCAGTATGTG GATGGCTCGT ACTTCTTACC TTGCTGCCTT CGTCATCATG 
GTTATATTTA CACTCTCAGT CTCAATGCTT CTGCGGTATT CCCATCATCA AATCTTTGTA 
TTTATTGTGG ACTTGCTACA GATGTTAGAG ATGAATATGA GTATCGCGTT TCCTGCTGCA 
CCATTGCTCA CTGTGATCCT GGCTCTTGTT GGGATGGAAG CCATCATGTC AGAATTTTTC 
AATGATACCA CCACTGCTTT CTATATAATC CTCATTGTGT GGTTAGCTGA TCAGTATGAT 
GCCATATGCT GCCATACAAA CACCAGCAAG AGACACTGGC TCAGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P