DCHS1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DCHS1
Entrez Gene ID	515576
Full Name	dachsous cadherin-related 1
General protein information	Preferred Names dachsous cadherin-related 1 Names protocadherin-16 dachsous 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002693149.5	XP_002693195.2	protocadherin-16
XM_002693149.4	XP_002693195.1	protocadherin-16
XM_002701345.4	XP_002701391.2	protocadherin-16

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DCHS1	XP_003313012.1
Bos taurus (cattle)	DCHS1	XP_002693195.1
Rattus norvegicus (Norway rat)	Dchs1	NP_001101014.2
Homo sapiens (human)	DCHS1	NP_003728.1
Danio rerio (zebrafish)	dchs1b	XP_001921284.3
	DCHS1	XP_001100797.1
Mus musculus (house mouse)	Dchs1	NP_001156415.1
Gallus gallus (chicken)	DCHS1	XP_417264.4
Caenorhabditis elegans (roundworm)	cdh-1	NP_497641.2
Canis lupus familiaris (dog)	DCHS1	XP_853733.2
Drosophila melanogaster (fruit fly)	ds	NP_523446.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007905	XP_317578.4
Danio rerio (zebrafish)	LOC100334632	XP_002664697.2

Gene Ontology

The following DCHS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DCHS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa223264	XM_002693149.5 Latest version!	Bos taurus dachsous cadherin-related 1 (DCHS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa26442	XM_002701345.4 Latest version!	Bos taurus dachsous cadherin-related 1 (DCHS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa26442	XM_002693149.4 Latest version!	Bos taurus dachsous cadherin-related 1 (DCHS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DCHS1 ( XM_002693149.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002693195.2 Bos taurus dachsous cadherin-related 1 (DCHS1), mRNA.

Price & Availability↑

CloneID	OBa223264
Clone ID Related Accession (Same CDS sequence)	XM_002693149.5
Accession Version	XM_002693149.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protocadherin-16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002693149.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG AGTGGGCCAC TGCACCTTCG TGCCCGGGCA TGCAGATCCC CAGGCCTCTC 
CCCCTGCTGT CATTGCTGAT GCTGCTGCTC CTGGGGGCGG GGGCTCCAGG TGCCTGGGGT 
CAGGCTGGGA GCTTGGACCT GCAGATTGAT GAGGAGCAGC CAGCGGGCAC GCTGATCGGG 
GACATCAGTG CAGGGCTTCC AGCGGGCACA GCAGCACCTC CCATGTACTT CATCTCTGCC 
CAGGAGGGCA GCGGGGTGGG CACGGACCTG GCCATCGATG AACACAGTGG GGTGGTCCGT 
ACAGCCCGTG TCTTGGACCG AGAGCGGCGG GATCGCTACC GCTTCACTGC AGTCACTCCC 
GACGGTGCCA CTGTGGAAGT TACCGTGCGA GTGGCTGACA TCAATGACCA TGCTCCAGCT 
TTCCCACAGG CTCGGGCTGT CCTGCAGATA CCTGAGCATA CAGCACTTGG CACCCGCTAC 
CCACTGGAGC CTGCTCGTGA TGCCGACGCT GGCCGCCTGG GAACGCAGGG CTATGCCCTG 
TCTGGTGATG GGGCTGGAGA GACCTTCCGG CTGGAGACAC GCCCTGGTCC AGATGGGGCC 
CCAGTGCCTG AGCTGGTAGT TACCGGAGAG CTGGACCGAG AGAACCGCTC ACACTACATG 
CTGCAACTGG AGGCCTATGA CGGTGGTTCA CCCCCTCGGA GGGCCCAGGC CCTGCTGGAC 
GTGACACTGC TAGACATCAA TGACCATGCC CCAGCTTTCA ATCAGAGCCG CTACCACGCT 
GCTGTGTCCG AGAGCCTGGC CCCCGGTAGT CCTGTCTTGC AGGTGTATGC CTCTGATGCC 
GATGCAGGTG CCAATGGGGC TGTAACTTAT GAGATCAACC GGAGGCAGAG TGAGGGTGAT 
GGACCCTTCT CCATTGATGC ACACACGGGG CTGCTGCGGC TGGAGCGGCC GCTGGACTTT 
GAGCAACGTC GGGTCCACGA ACTGGTGGTG CAGGCTCGGG ATGGAGGGGC TCACCCTGAG 
CTGGGCTCAG CCTTTGTGAC CGTGCATGTG CGAGATGCCA ATGACAATCA GCCCTCCATG 
ACTGTCATCT TCCTGAGTGC AGATGGCTCC CCCCGTGTAT CTGAGGCTGC CCCCCCGGGC 
CAGCTTGTTG CTCGAATCTC TGTGTCAGAC CCAGATGACG GTGACTTTGC GCATGTCAAC 
GTGTCCCTGG AGGGTGGAGA GGGCCACTTT GCGCTCAGCA CCCAGGACAG CGTCATCTAC 
CTGGTGTGTG TGGCTCGGAG GCTGGATCGG GAGGAGCGCG ACGCCTACAA CTTGCGAGTT 
ACTGCCACAG ACTCAGGCTC ACCCCCACTG CGGGCCGAGG CTGCCTTTGT GCTACACGTC 
ACTGATGTCA ATGACAATGC GCCTGCCTTT GACCGCCAGC TCTACCGACC CGAGCCTCTG 
CCTGAGGTTG CCCTGCCTGG CAGCTTCGTG GCGCGGGTGA CGGCCCGGGA TCCTGACCAG 
GGCACCAATG GTCAGGTCAC CTACAGCCTG GCCCCAGGCG CCCACACCCG CTGGTTCTCC 
ATAGACCCCA CCTCAGGCAT TGTCACTACA GCTGCTTTGC TGGACTATGA GTTGGAACCT 
CAGCCACAGC TAATCGTGGT GGCCACGGAT GGGGGCTTGC CCCCTCTCTC CTCCTCTGCC 
ACAGTGAGCG TGGTCTTGCA AGATGTGAAT GATAACGAGC CGCAGTTCCA GAGGACTTTC 
TACAACGCCT CGCTGCCTGA GGGCACTCAG CCAGGAACCT GCTTCCTGCA GGTGACAGCC 
ACAGATGCAG ACAGTGGCCC ATTTGGCCTC CTCTCCTATT CCTTGGGTGC CGGACTTGGG 
ACCTCAGGGT CTCCCCCGTT CCGCATCGAT GCCCACAGTG GTGACGTGTG TACGACCCGG 
ACCCTGGACC GCGATCAGGG GCCCTCAAGC TTTGACTTCA CAGTGACAGC TGTGGATGGG 
GGAGGCCTCA AGTCTATGGT ATATGTGAAG GTGTTTGTGT CAGACGAGAA TGACAACCCA 
CCTCAGTTTT ATCCACGGGA GTATGCTGCC AGTCTGAGCG CCCAGAGTGC ACCAGGCACA 
GCTGTGCTGA GGGTACGTGC CCATGACCCT GATCAGGGAC CCCATGGGCG ACTCTCCTAC 
CACATCCTGG CTGGCAACAG CCCCCCGCTC TTTGCCTTGG ACGAGCACTC AGGACTATTG 
ACAGTAGCCT GGCCCTTGGC CAGACGGGCC AGTTCCATGG TGCAGCTGGA GATCGGGGCT 
CGGGATGGAG GTGGCCTGCA GGCGGAGCCC AATGCCCGAG TCAACATCAG CATTGTGCCT 
GGAACTCCTG TACCACCAGT ATTTGAGCAG CTTCAGTATG TCTTTTCTGT GCCAGAGGAT 
GTGGCACCAG GCACCAGTGT GGGCGTAGTC CAGGCACACA ATCCACCAGG TCGCTTGGGA 
CCGGTGACCC TTGCCCTATC GGGGGGCGAT CCCCGAGGAC TCTTCTCCCT AGATGGGGCC 
ACTGGGCTGT TACAGACACT TCGCCCTCTG GACCGGGAGC TGCTGGGACC AGTGCTGGAG 
CTGGAGGTGC GGGCAGGCAG CGGAGTGCCA CCGGCTTTCG CTGTAGCTCG GGTGCGCGTG 
CTGCTGGACG ATGTGAATGA CAACTCCCCT GCCTTCCCTG CGCCTGAAGA CACAGTGCTG 
CTGCCATCGA GCACTGCCCC AGGGACCCTC ATCTATACAC TGCGGGCTCT GGACCCCGAC 
TCAGGAGTTA ACAGTCGAGT CACCTTTACC CTGCTTGCTG GGGGTGGTGG GACCTTCACT 
GTGGACCCCA CCACAGGCCA CATACGGCTT ATGGGACCTC TGGGGCCTCC GGGGGGGCCA 
GCCCATGAAC TGGAGCTGGA AGCCCGGGAT GGAGGCTCCC CACCTCGCAC CAGCCACTTT 
CGACTGCGGG TGGTGGTACA GGACTTGGGG ACCCGTGGGC TGGCTCCCCA CTTCGACAGC 
CCTACCTACC GTGTAGACCT GCCCTCAGGC ACCACCCCTG GAACTCAAGT CCTACAAGTG 
CAGGCTCGAG CACCAGATGG GAGCCCTGTC ACCTACCACC TTGCAGCAGA TGGGCCAGGT 
AGCCCCTTTG GCCTGGAACC ACAGAGTGGA TGGCTGTGGG TGCGGGCAGC ACTGGACCAT 
GAGGCCCAGG AGTTGTACAC ACTGAAAGTA ATGGCAGTGT CTGGATCCAA AGCTGAGTTG 
GGGCAGCAGA CAGCCACGGC CACCGTGAGG ATCAGCATCC TCAACCAGAA CGACCACAGT 
CCCCGCTTGT CTGAGGAGCC CACCTTCCTG GCTGTGGCTG AGAACCAGCC CCCAGGGACA 
AGCGTGGGTC GGGTCTTTGC CACTGACCGA GATTCAGGAA TCAACGGACG TCTGACCTAC 
AGCCTGCGAC AGCTGTCAGA AGACAGCAAG GCCTTCCGCA TCCACCCCCA GACTGGAGAG 
GTGACCACAC TACAAACTCT GGACCGTGAG CGGCAGAACA GCTACCAGCT TCTGGTGCAG 
GTTCAGGATG CAGGGAGCCC ACCCCGCAGC ACCACAGGCA CCGTGCACAT CGCAGTGCTT 
GACCTCAATG ACAACAGCCC TACCTTCCTG CAGGCCTCAG GGGCTGCTGG TGGAGGCCTC 
CCCATACAGG TACCAGATGG TGTGCCTCCA GGAACATTGG TGACAACCCT GCAGGCCAAG 
GATCCAGATG AGGGGGAGAA TGGAACCATC CTGTACATGC TAACTGGTCC TGGCTCAGAG 
CTCTTCTCCC TGCACCCTCA CTCGGGAGAG CTGCTCACGG CAGCACCCCT GATCCGAGCA 
GAGCGGCCAC ACTACGTGCT CACATTGAGC GCTCATGACC AAGGCAGCCC CCCTCGGAGC 
TCCAGCCTCC AGCTGCTAGT GCAGGTGCTT CCCTCCACTC GCTCGGCTGA GCCTGCACCG 
GAACCCTCAG AGCCAGACCC GGTGGCGCCT GTGCCTGTCG TGCTGACCGT GACAGCAGCC 
GAGGGGCTCC GGCCCGGCTC CCTGTTGGGC TCTGTGGCAC CGCTGGAGCA GGCGGGGGTG 
GGCGCCCTTA CCTACACACT GGTGGGCGGC GCCGACCCAG AGGGCACCTT TGCGCTGGAC 
GCAGCCTCTG GGCGCTTGTA CCTGGCGCGG CCCCTGGACT TCGAGGCGGG TCCGGCTTGG 
CGCGCGCTCA CGGTGCGCGC CGAGGGGCCG GGAGGCGCGG GCGCGCGGCT GCTGAGAGTG 
CAGGTGCGCG TGCAGGACGA GAACGAGCAC GCGCCGGCCT TCGCGCGCGA CCCGCTGGCG 
TTGGCGCTCC CAGAGAACCC GGAGCCGGGC GCGACCTTGT ACACTTTCCG CGCGTCCGAC 
GCCGACGGCC CCGGCCCCAA CAGCGATGTG CGCTACCGCC TGCTGCGCCA AGAGCCGCCT 
GTGCCCGCGC TTCGCCTGGA CGCGCGCACC GGAGCGCTCA GCGCCCCGCG AGGCCTGGAT 
CGGGAGACCA CTCCGGCTCT GCTGCTGCTC GTGGAAGCCA CTGACCGGCC CGCCAACGCC 
AGCCGCCGCC GCACAGCACG CGTTTCCGCG CGCGTCTTTG TCACGGATGA GAATGACAAT 
GCTCCAGTCT TTGCCTCGCC CTCACGTGTG CGCCTCCCTG AGGATCACCC GCCCGGGCCC 
ACGGCGCTGC ACGTGGTAGC CCGGGACCCC GACCTGGGCG AGGCCGCACG GGTGTCCTAT 
CGCCTGGCAG CTGGCGCGGA CGGCTGTTTC CGGCTACACC CCAGCACCGG AGCGCTGTCC 
GTGGTGCGGT CTCTGGACCG CGAACAGCGA GCTGAGTACG TGCTGACTGT GGTGGCCTCT 
GACCACGGCT CCCCACCGCG CTCGGCCACG CAGCTTCTGA CTGTCAGTGT CGCCGACGTC 
AACGACGAGG CACCCGCTTT CCAACAGCAG GAGTACAGCG TCCTCTTGCG GGAGAACAGC 
CCGCCTGGAA CGTCTCTGCT CACTCTGCAG GCAACCGACC CCGATCTGGG GGCCAATGGG 
CAAGTGACTT ATGGAGGCAT CTCTGGCGAA AACTTCTCCC TGGATCCAGA CACCGGGGTC 
CTTACCACTC TTCGGGCTCT GGATCGGGAG GAGCAGGAGG AGATCAACTT GACAGTATAT 
GCCCGGGACG GGGGCTCGCC TCCACTGTTG ACCCACGTCA CAGTGCGAGT GGCTGTGGAG 
GATGAGAATG ACCATGCCCC AACCTTTGGG AGTGCCCATC TCTCCCTGGA GGTGCCCGAG 
GGCCAGGACC CCCAGACCCT TACCACGCTG CGGGCCTCTG ACCCGGATGT GGGAGCCAAC 
GGGCAGCTGC AGTACCGCAT CCTGGATGGG GACCCCTCAG GAGCCTTTGT CCTGGACCTA 
GCTTCTGGGG AGTTTGGCAC CATGAGGCCA CTAGACCGGG AGGTGGAGCC AGCATTCCAG 
CTGCAGATAG AAGCCCGGGA TGGAGGCCAG CCAGCTCTCA GCGCTACGCT TCTTGTGACA 
GTGACAGTGC TAGATGCCAA TGACCATGCC CCAGCCTTCC CTGTGCCCTC CTACTCTGTG 
GAGGTGCCTG AGGACGCACC TGCAGGGACC CTGCTGCTGC AGCTGCAGGC TCATGATCCT 
GATGCTGGGG CCAATGGTCG TGTGACCTAC TACCTGGGTA CAGGTGCAGC AGGAGCCTTC 
CTGCTAGAGC CCAGCTCTGG GGAATTGCGC ACAGCCACCA CCCTTGACAG AGAGCAGTGT 
CCCAGCTATG CCTTTACAGT GAATGCAGTG GACAGTGCAG CTGCTGGGCC CCTAAGCACC 
ACAGTGCCCG TCACCATCAC AGTGCGTGAT GTCAATGACC ACGCGCCCAC CTTCCCCACC 
AGTCCCCTGA GGCTGCGCCT GCCCCGCCCA GGCCCTGTCT TCAGCACTCC AGTTCTGCCT 
CTGGCCACTC TGCGAGCTGA AGACCGTGAC ACTGGTGCCA ATGCCTCCAT CCTGTACCGG 
CTGGCAGGCA CACCACCTCC TGGCACCACC GTGGACTCTT ACACTGGTGA AATCCGTGTG 
TCCCGCTCCC CTGTATCTCT GAGCCCCCGA GATCGTGTCC TCTTCATTGT GGCAACTGAC 
CTTGGCCGTC CAGCTCGCTC TGCCACTGGT GTGGTCATTA TTGGGCTGCA AGGGGAGTCT 
GAGCATGGAC CCCGCTTCTC CCGAGCCAGT AGTGAAGCCA TGCTCCGTGA GAATGCACCC 
CCAGGGACTC CCGTTGTCTC CCCCAAGGCT ATCCATGCAG GGGGCTCGAG TGGACCCATC 
ACATACAGCA TTCTCAGTGG GAATGAGAAA GGGACATTCT CCATCCAGCC TAGTACAGGA 
GCCATCACAG TCCGCTCAGC AGAGGGGCTG GATTTTGAGG CAAACCCACG GCTGCGACTG 
GTGCTGCAGG CAGAGAGTGG AGGAGCCTAT GCCTTCTCTG TGCTGACCCT AACCCTGCAA 
GATGCCAATG ACAACGCGCC CCGCTTCCTG CAGCCCCACT ACGTGGCCTT CCTTCCAGAG 
TCCAGGCCCT TGGAGGGACC CCTGCTACAG GTGGAAGCAG ATGACCTGGA CCAAGGCCCC 
AGTGGACAGA TCTCCTACAG TCTGGCCGCA TCCCAGCCAG CCCGGGGATT GTTCCATGTA 
GACCCAGCCA CGGGCACTAT CACCACCACA GCTATCCTGG ACCGCGAGAT CTGGGCCGAA 
ACACGGCTGG TGCTGATGGC CACAGACAGA GGAAGCCCAG CCCTGGTGGG CTCAGCTACC 
CTGACGGTGA TGGTCATCGA CACCAATGAC AATCGCCCCA CCATCCCCCA GCCCTGGGAG 
CTCCGAGTGT CAGAAGATGC ACTGCTGGGC TCGGAGATCG CACAGGTAAC GGGGAATGAT 
GTGGACTCAG GACCAGTGCT GTGGTATGTT CTGAGCCCTT CTGGGCCCCA GGATCCCTTC 
AGTGTCGGCC GCTATGGAGG CCGCCTCTCC CTCACGGGCC CCTTGGATTT TGAGCAGCGC 
GACCGCTACC ACCTGCAGCT CCTGGCACAC GACGGGCCTC ACGAGGGCCA GGCCAACCTC 
ACGGTGTTCG TGGAGGATGT CAATGACAAC TCCCCTGCCT TCTCACAGAC TCTCTACCAG 
GTGATGCTGC TCGAGCACAC ACCCCCGGGC AGTGCCATTC TCTCCGTCTC GGCCACTGAC 
CGGGACTCGG GCGCCAACGG TCACGTCTCC TACCACCTGG CTTCCTCTGC TGAGGGCTTC 
AGCATTGACC CCAACAACGG GACCCTATTC ACAACAGTGG GGACAGTGGC CTTGGGCCAT 
GAAGGGCCAG GCATGGTGGA CGTGGTGCTG GAAGCCCGAG ACCACGGAGT GCCGGTTCGG 
GCCGCCCGAG CCACAGTGCA CGTGCAGCTG CAGGATCAGA ACGACCATGC CCCGAGCTTC 
ACGCTGCCAC ACTACCGCGT GGCTGTGACT GAGGATCTGC CGCCCGGCTC CACCCTGCTC 
ACCCTGGAGG CCACAGATGC TGACGGGAGC CGCAGCCATG CCACCGTGGA CTACAGCATT 
GTCAGTGGCA ACCGGGGCCG AGTCTTTCAG CTGGAACCCC GGCTGGCCGA GGCTGGGGAG 
AGTGATGGAC TAGGCCCCCG GGCCCTGGGC TGCCTGGTGT TGCTTGAGCC TCTAGACTTT 
GAAAGCCTAA CCCAATACAA CCTAACTGTG GCAGCAACTG ACCGGGGGCA GCCACCTCGA 
AGTTCGGCTG TGCCAGTCAC CGTCAGCGTG CTGGATGTCA ACGACAACCC ACCTGTCTTC 
ACCCGAGCAG CCTACCGTGT GGCGGTGCCT GAGGACACGC CTGTGGGAGC CGAGCTGCTG 
CACGTGGAGG CCTCCGACGC TGATCCAGGC CCCCATGGCC TTGTACGCTT CACCCTCAGC 
TCAGGCGACC CTCTGGGGCT CTTTGAGCTG GATGAGAGCT CCGGGGACTT GCGACTGGCC 
CGCCCGTTGG ACTGTGAGAC CCAAGCTCGA CATCAGCTTG TAGTGCAGGC TTCTGACCCT 
GCCGGGGTAC ACTTTGCTCT GGCACCAGTG ACCATTGAGG TCCAGGACGT GAATGACCAT 
GGCCCCGCCT TCCCTCTGAG CTTGCTCAGC ACCAGCCTGG CTGAGAACCA GCCTCCAGGC 
ACTCTTGTTA CCACCCTGCA TGCAATTGAT GGGGATGCTG GGGCTTTCGG AAGGCTCCAC 
TACAGCCTGC TGGAGGCTGG GCCGGGGCCC GAGGGCCGCG AGGCATTTGC GCTCAACAGC 
TCAACTGGGG AGTTGCGGGC ACGAGTGGCC TTTGACTACG AGCACACTGG CAGCTTCCAG 
CTGCTGGTGG GCGCTACAGA TGCCGGGAAC CTGTCCGCCT CAGTCACTGT GTCAGTGCTG 
GTGACCGGTG AGGATGAGTA TGACCCAGTG TTCCTGGCTC CAGCTTTCCA CTTTCAGGTG 
CCAGAAGGTG CACGGCGTGG CCACAGCCTG GGTCACGTGC AGGCCACAGA CGAGGATGGA 
GGTGCTGACG GCCTGGTGCT GTACTCGCTG GCCGCCTCGT CCCCCTATTT TGGTATCAAC 
CAGACTACAG GTGCCCTGTA CCTGCGGGTG GACAGCCGGG CACCAGGCAG TGGAACAGGC 
ACCTCCGGGG GTGGGGGCCG GACCCGACGT GAGGCGCCAC GGGAGCTGAG GCTGGAGGTG 
GTGGCAAGGG GGCCTCTGCC CGGTTCTCGG AGTGCCACCG TGCCTGTGAC TGTGGACATC 
ACCCACACTG CGCTGGGCCT GGCACCTGAC CTCAACCTGC TCTTGGTGGG AGCCGTGGCA 
GCCTCTCTGG GAGTCGTGGT GGTGCTTGCC CTGGCAGCCC TTGTCCTGGG ACTGGTGCGG 
GCCCGGAGCC GCAAAGCTGA GGCGGCGCCT GGCCCGATGT CCCAGGCGGC ACCCCTGGCC 
AGTGGCTCAC TGCAGAAGCT GGGCCGAGAG CCACCCAGCC CGCCGCCCTC AGAGCACCTG 
TATCACCAGA CCCTCCCCAG CTATGGTGGG CCTGGAGCTG GAGGACCCTA CCCCCGCGGT 
GGCTCCCTGG ACCCTTCACA CTCAAGCGGC CGAGGCTCAG CAGAGGCTGC GGAAGATGAC 
GAGATCCGCA TGATCAACGA GTTCCCCCGA GTGGCCAGTG TGGCCTCCTC CCTGGCTGCC 
CGCGGCCCTG ACTCCGGCAT CCAGCAGGAT GCAGACGGAC TGAGTGACAC CTCCTGTGAG 
CCACCTGCCC CTGACACCTG GTATAAGGGC CGTAAGGCAG GGCTGCTGCT GCCGGGTGCA 
GGAGCCACTC TGTACAGAGA AGACGGCCCC CCAGCCACTG CCACCACCTT CCTGGGGGGC 
TGTGGCCTAA GCCCCGCGCC CACCGGGGAC TACGGCTTTC CAGCAGATGG CAAGCCCTGT 
GTGGCGGGAG CGCTGACGGC CATCGTGGCC GGTGAGGAGG AGCTCCGTGG CAGCTACAAC 
TGGGACTACC TGCTGAGCTG GTGCCCTCAG TTCCAGCCGC TGGCCAGTGT CTTCACAGAG 
ATTGCCCGGC TCAAGGATGA AGCTCGGCCA TGTCCCCCGG CGCCCCGTAT TGACCCACCG 
CCCCTCATCA CTGCCGTGGC CCACCCAGGA GCCAAGTCTG TGCCCCCCAA ACCAGCCAGC 
ACGGCCGCAA CCCGGGCCGT CTTCCCACCA GCCTCTCACC GCTCCCCCAT CAGCCACGAA 
GGTTCCCTGT CCTCGGCGGC CATGTCTCCC AGCTTCTCAC CTTCACTATC TCCTCTGGCC 
GCTCGCTCAC CCGTCGTCTC ACCGTTTGGG GTGGCCCAGG GCCCCTCAGC CTCAGCTCTG 
AGCGCAGAGT CTGGCCTGGA GCCACCGGAC GACACCGAGC TCCATATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002693195.2
CDS	332..10222
Translation	MQKEWATAPSCPGMQIPRPLPLLSLLMLLLLGAGAPGAWGQAGSLDLQIDEEQPAGTLIGDISAGLPAGTAAPPMYFISAQEGSGVGTDLAIDEHSGVVRTARVLDRERRDRYRFTAVTPDGATVEVTVRVADINDHAPAFPQARAVLQIPEHTALGTRYPLEPARDADAGRLGTQGYALSGDGAGETFRLETRPGPDGAPVPELVVTGELDRENRSHYMLQLEAYDGGSPPRRAQALLDVTLLDINDHAPAFNQSRYHAAVSESLAPGSPVLQVYASDADAGANGAVTYEINRRQSEGDGPFSIDAHTGLLRLERPLDFEQRRVHELVVQARDGGAHPELGSAFVTVHVRDANDNQPSMTVIFLSADGSPRVSEAAPPGQLVARISVSDPDDGDFAHVNVSLEGGEGHFALSTQDSVIYLVCVARRLDREERDAYNLRVTATDSGSPPLRAEAAFVLHVTDVNDNAPAFDRQLYRPEPLPEVALPGSFVARVTARDPDQGTNGQVTYSLAPGAHTRWFSIDPTSGIVTTAALLDYELEPQPQLIVVATDGGLPPLSSSATVSVVLQDVNDNEPQFQRTFYNASLPEGTQPGTCFLQVTATDADSGPFGLLSYSLGAGLGTSGSPPFRIDAHSGDVCTTRTLDRDQGPSSFDFTVTAVDGGGLKSMVYVKVFVSDENDNPPQFYPREYAASLSAQSAPGTAVLRVRAHDPDQGPHGRLSYHILAGNSPPLFALDEHSGLLTVAWPLARRASSMVQLEIGARDGGGLQAEPNARVNISIVPGTPVPPVFEQLQYVFSVPEDVAPGTSVGVVQAHNPPGRLGPVTLALSGGDPRGLFSLDGATGLLQTLRPLDRELLGPVLELEVRAGSGVPPAFAVARVRVLLDDVNDNSPAFPAPEDTVLLPSSTAPGTLIYTLRALDPDSGVNSRVTFTLLAGGGGTFTVDPTTGHIRLMGPLGPPGGPAHELELEARDGGSPPRTSHFRLRVVVQDLGTRGLAPHFDSPTYRVDLPSGTTPGTQVLQVQARAPDGSPVTYHLAADGPGSPFGLEPQSGWLWVRAALDHEAQELYTLKVMAVSGSKAELGQQTATATVRISILNQNDHSPRLSEEPTFLAVAENQPPGTSVGRVFATDRDSGINGRLTYSLRQLSEDSKAFRIHPQTGEVTTLQTLDRERQNSYQLLVQVQDAGSPPRSTTGTVHIAVLDLNDNSPTFLQASGAAGGGLPIQVPDGVPPGTLVTTLQAKDPDEGENGTILYMLTGPGSELFSLHPHSGELLTAAPLIRAERPHYVLTLSAHDQGSPPRSSSLQLLVQVLPSTRSAEPAPEPSEPDPVAPVPVVLTVTAAEGLRPGSLLGSVAPLEQAGVGALTYTLVGGADPEGTFALDAASGRLYLARPLDFEAGPAWRALTVRAEGPGGAGARLLRVQVRVQDENEHAPAFARDPLALALPENPEPGATLYTFRASDADGPGPNSDVRYRLLRQEPPVPALRLDARTGALSAPRGLDRETTPALLLLVEATDRPANASRRRTARVSARVFVTDENDNAPVFASPSRVRLPEDHPPGPTALHVVARDPDLGEAARVSYRLAAGADGCFRLHPSTGALSVVRSLDREQRAEYVLTVVASDHGSPPRSATQLLTVSVADVNDEAPAFQQQEYSVLLRENSPPGTSLLTLQATDPDLGANGQVTYGGISGENFSLDPDTGVLTTLRALDREEQEEINLTVYARDGGSPPLLTHVTVRVAVEDENDHAPTFGSAHLSLEVPEGQDPQTLTTLRASDPDVGANGQLQYRILDGDPSGAFVLDLASGEFGTMRPLDREVEPAFQLQIEARDGGQPALSATLLVTVTVLDANDHAPAFPVPSYSVEVPEDAPAGTLLLQLQAHDPDAGANGRVTYYLGTGAAGAFLLEPSSGELRTATTLDREQCPSYAFTVNAVDSAAAGPLSTTVPVTITVRDVNDHAPTFPTSPLRLRLPRPGPVFSTPVLPLATLRAEDRDTGANASILYRLAGTPPPGTTVDSYTGEIRVSRSPVSLSPRDRVLFIVATDLGRPARSATGVVIIGLQGESEHGPRFSRASSEAMLRENAPPGTPVVSPKAIHAGGSSGPITYSILSGNEKGTFSIQPSTGAITVRSAEGLDFEANPRLRLVLQAESGGAYAFSVLTLTLQDANDNAPRFLQPHYVAFLPESRPLEGPLLQVEADDLDQGPSGQISYSLAASQPARGLFHVDPATGTITTTAILDREIWAETRLVLMATDRGSPALVGSATLTVMVIDTNDNRPTIPQPWELRVSEDALLGSEIAQVTGNDVDSGPVLWYVLSPSGPQDPFSVGRYGGRLSLTGPLDFEQRDRYHLQLLAHDGPHEGQANLTVFVEDVNDNSPAFSQTLYQVMLLEHTPPGSAILSVSATDRDSGANGHVSYHLASSAEGFSIDPNNGTLFTTVGTVALGHEGPGMVDVVLEARDHGVPVRAARATVHVQLQDQNDHAPSFTLPHYRVAVTEDLPPGSTLLTLEATDADGSRSHATVDYSIVSGNRGRVFQLEPRLAEAGESDGLGPRALGCLVLLEPLDFESLTQYNLTVAATDRGQPPRSSAVPVTVSVLDVNDNPPVFTRAAYRVAVPEDTPVGAELLHVEASDADPGPHGLVRFTLSSGDPLGLFELDESSGDLRLARPLDCETQARHQLVVQASDPAGVHFALAPVTIEVQDVNDHGPAFPLSLLSTSLAENQPPGTLVTTLHAIDGDAGAFGRLHYSLLEAGPGPEGREAFALNSSTGELRARVAFDYEHTGSFQLLVGATDAGNLSASVTVSVLVTGEDEYDPVFLAPAFHFQVPEGARRGHSLGHVQATDEDGGADGLVLYSLAASSPYFGINQTTGALYLRVDSRAPGSGTGTSGGGGRTRREAPRELRLEVVARGPLPGSRSATVPVTVDITHTALGLAPDLNLLLVGAVAASLGVVVVLALAALVLGLVRARSRKAEAAPGPMSQAAPLASGSLQKLGREPPSPPPSEHLYHQTLPSYGGPGAGGPYPRGGSLDPSHSSGRGSAEAAEDDEIRMINEFPRVASVASSLAARGPDSGIQQDADGLSDTSCEPPAPDTWYKGRKAGLLLPGAGATLYREDGPPATATTFLGGCGLSPAPTGDYGFPADGKPCVAGALTAIVAGEEELRGSYNWDYLLSWCPQFQPLASVFTEIARLKDEARPCPPAPRIDPPPLITAVAHPGAKSVPPKPASTAATRAVFPPASHRSPISHEGSLSSAAMSPSFSPSLSPLAARSPVVSPFGVAQGPSASALSAESGLEPPDDTELHI

Target ORF information:

RefSeq Version	XM_002693149.5
Organism	Bos taurus(cattle)
Definition	Bos taurus dachsous cadherin-related 1 (DCHS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002693149.5

ORF Insert Sequence:

ATGCAGAAGG AGTGGGCCAC TGCACCTTCG TGCCCGGGCA TGCAGATCCC CAGGCCTCTC 
CCCCTGCTGT CATTGCTGAT GCTGCTGCTC CTGGGGGCGG GGGCTCCAGG TGCCTGGGGT 
CAGGCTGGGA GCTTGGACCT GCAGATTGAT GAGGAGCAGC CAGCGGGCAC GCTGATCGGG 
GACATCAGTG CAGGGCTTCC AGCGGGCACA GCAGCACCTC CCATGTACTT CATCTCTGCC 
CAGGAGGGCA GCGGGGTGGG CACGGACCTG GCCATCGATG AACACAGTGG GGTGGTCCGT 
ACAGCCCGTG TCTTGGACCG AGAGCGGCGG GATCGCTACC GCTTCACTGC AGTCACTCCC 
GACGGTGCCA CTGTGGAAGT TACCGTGCGA GTGGCTGACA TCAATGACCA TGCTCCAGCT 
TTCCCACAGG CTCGGGCTGT CCTGCAGATA CCTGAGCATA CAGCACTTGG CACCCGCTAC 
CCACTGGAGC CTGCTCGTGA TGCCGACGCT GGCCGCCTGG GAACGCAGGG CTATGCCCTG 
TCTGGTGATG GGGCTGGAGA GACCTTCCGG CTGGAGACAC GCCCTGGTCC AGATGGGGCC 
CCAGTGCCTG AGCTGGTAGT TACCGGAGAG CTGGACCGAG AGAACCGCTC ACACTACATG 
CTGCAACTGG AGGCCTATGA CGGTGGTTCA CCCCCTCGGA GGGCCCAGGC CCTGCTGGAC 
GTGACACTGC TAGACATCAA TGACCATGCC CCAGCTTTCA ATCAGAGCCG CTACCACGCT 
GCTGTGTCCG AGAGCCTGGC CCCCGGTAGT CCTGTCTTGC AGGTGTATGC CTCTGATGCC 
GATGCAGGTG CCAATGGGGC TGTAACTTAT GAGATCAACC GGAGGCAGAG TGAGGGTGAT 
GGACCCTTCT CCATTGATGC ACACACGGGG CTGCTGCGGC TGGAGCGGCC GCTGGACTTT 
GAGCAACGTC GGGTCCACGA ACTGGTGGTG CAGGCTCGGG ATGGAGGGGC TCACCCTGAG 
CTGGGCTCAG CCTTTGTGAC CGTGCATGTG CGAGATGCCA ATGACAATCA GCCCTCCATG 
ACTGTCATCT TCCTGAGTGC AGATGGCTCC CCCCGTGTAT CTGAGGCTGC CCCCCCGGGC 
CAGCTTGTTG CTCGAATCTC TGTGTCAGAC CCAGATGACG GTGACTTTGC GCATGTCAAC 
GTGTCCCTGG AGGGTGGAGA GGGCCACTTT GCGCTCAGCA CCCAGGACAG CGTCATCTAC 
CTGGTGTGTG TGGCTCGGAG GCTGGATCGG GAGGAGCGCG ACGCCTACAA CTTGCGAGTT 
ACTGCCACAG ACTCAGGCTC ACCCCCACTG CGGGCCGAGG CTGCCTTTGT GCTACACGTC 
ACTGATGTCA ATGACAATGC GCCTGCCTTT GACCGCCAGC TCTACCGACC CGAGCCTCTG 
CCTGAGGTTG CCCTGCCTGG CAGCTTCGTG GCGCGGGTGA CGGCCCGGGA TCCTGACCAG 
GGCACCAATG GTCAGGTCAC CTACAGCCTG GCCCCAGGCG CCCACACCCG CTGGTTCTCC 
ATAGACCCCA CCTCAGGCAT TGTCACTACA GCTGCTTTGC TGGACTATGA GTTGGAACCT 
CAGCCACAGC TAATCGTGGT GGCCACGGAT GGGGGCTTGC CCCCTCTCTC CTCCTCTGCC 
ACAGTGAGCG TGGTCTTGCA AGATGTGAAT GATAACGAGC CGCAGTTCCA GAGGACTTTC 
TACAACGCCT CGCTGCCTGA GGGCACTCAG CCAGGAACCT GCTTCCTGCA GGTGACAGCC 
ACAGATGCAG ACAGTGGCCC ATTTGGCCTC CTCTCCTATT CCTTGGGTGC CGGACTTGGG 
ACCTCAGGGT CTCCCCCGTT CCGCATCGAT GCCCACAGTG GTGACGTGTG TACGACCCGG 
ACCCTGGACC GCGATCAGGG GCCCTCAAGC TTTGACTTCA CAGTGACAGC TGTGGATGGG 
GGAGGCCTCA AGTCTATGGT ATATGTGAAG GTGTTTGTGT CAGACGAGAA TGACAACCCA 
CCTCAGTTTT ATCCACGGGA GTATGCTGCC AGTCTGAGCG CCCAGAGTGC ACCAGGCACA 
GCTGTGCTGA GGGTACGTGC CCATGACCCT GATCAGGGAC CCCATGGGCG ACTCTCCTAC 
CACATCCTGG CTGGCAACAG CCCCCCGCTC TTTGCCTTGG ACGAGCACTC AGGACTATTG 
ACAGTAGCCT GGCCCTTGGC CAGACGGGCC AGTTCCATGG TGCAGCTGGA GATCGGGGCT 
CGGGATGGAG GTGGCCTGCA GGCGGAGCCC AATGCCCGAG TCAACATCAG CATTGTGCCT 
GGAACTCCTG TACCACCAGT ATTTGAGCAG CTTCAGTATG TCTTTTCTGT GCCAGAGGAT 
GTGGCACCAG GCACCAGTGT GGGCGTAGTC CAGGCACACA ATCCACCAGG TCGCTTGGGA 
CCGGTGACCC TTGCCCTATC GGGGGGCGAT CCCCGAGGAC TCTTCTCCCT AGATGGGGCC 
ACTGGGCTGT TACAGACACT TCGCCCTCTG GACCGGGAGC TGCTGGGACC AGTGCTGGAG 
CTGGAGGTGC GGGCAGGCAG CGGAGTGCCA CCGGCTTTCG CTGTAGCTCG GGTGCGCGTG 
CTGCTGGACG ATGTGAATGA CAACTCCCCT GCCTTCCCTG CGCCTGAAGA CACAGTGCTG 
CTGCCATCGA GCACTGCCCC AGGGACCCTC ATCTATACAC TGCGGGCTCT GGACCCCGAC 
TCAGGAGTTA ACAGTCGAGT CACCTTTACC CTGCTTGCTG GGGGTGGTGG GACCTTCACT 
GTGGACCCCA CCACAGGCCA CATACGGCTT ATGGGACCTC TGGGGCCTCC GGGGGGGCCA 
GCCCATGAAC TGGAGCTGGA AGCCCGGGAT GGAGGCTCCC CACCTCGCAC CAGCCACTTT 
CGACTGCGGG TGGTGGTACA GGACTTGGGG ACCCGTGGGC TGGCTCCCCA CTTCGACAGC 
CCTACCTACC GTGTAGACCT GCCCTCAGGC ACCACCCCTG GAACTCAAGT CCTACAAGTG 
CAGGCTCGAG CACCAGATGG GAGCCCTGTC ACCTACCACC TTGCAGCAGA TGGGCCAGGT 
AGCCCCTTTG GCCTGGAACC ACAGAGTGGA TGGCTGTGGG TGCGGGCAGC ACTGGACCAT 
GAGGCCCAGG AGTTGTACAC ACTGAAAGTA ATGGCAGTGT CTGGATCCAA AGCTGAGTTG 
GGGCAGCAGA CAGCCACGGC CACCGTGAGG ATCAGCATCC TCAACCAGAA CGACCACAGT 
CCCCGCTTGT CTGAGGAGCC CACCTTCCTG GCTGTGGCTG AGAACCAGCC CCCAGGGACA 
AGCGTGGGTC GGGTCTTTGC CACTGACCGA GATTCAGGAA TCAACGGACG TCTGACCTAC 
AGCCTGCGAC AGCTGTCAGA AGACAGCAAG GCCTTCCGCA TCCACCCCCA GACTGGAGAG 
GTGACCACAC TACAAACTCT GGACCGTGAG CGGCAGAACA GCTACCAGCT TCTGGTGCAG 
GTTCAGGATG CAGGGAGCCC ACCCCGCAGC ACCACAGGCA CCGTGCACAT CGCAGTGCTT 
GACCTCAATG ACAACAGCCC TACCTTCCTG CAGGCCTCAG GGGCTGCTGG TGGAGGCCTC 
CCCATACAGG TACCAGATGG TGTGCCTCCA GGAACATTGG TGACAACCCT GCAGGCCAAG 
GATCCAGATG AGGGGGAGAA TGGAACCATC CTGTACATGC TAACTGGTCC TGGCTCAGAG 
CTCTTCTCCC TGCACCCTCA CTCGGGAGAG CTGCTCACGG CAGCACCCCT GATCCGAGCA 
GAGCGGCCAC ACTACGTGCT CACATTGAGC GCTCATGACC AAGGCAGCCC CCCTCGGAGC 
TCCAGCCTCC AGCTGCTAGT GCAGGTGCTT CCCTCCACTC GCTCGGCTGA GCCTGCACCG 
GAACCCTCAG AGCCAGACCC GGTGGCGCCT GTGCCTGTCG TGCTGACCGT GACAGCAGCC 
GAGGGGCTCC GGCCCGGCTC CCTGTTGGGC TCTGTGGCAC CGCTGGAGCA GGCGGGGGTG 
GGCGCCCTTA CCTACACACT GGTGGGCGGC GCCGACCCAG AGGGCACCTT TGCGCTGGAC 
GCAGCCTCTG GGCGCTTGTA CCTGGCGCGG CCCCTGGACT TCGAGGCGGG TCCGGCTTGG 
CGCGCGCTCA CGGTGCGCGC CGAGGGGCCG GGAGGCGCGG GCGCGCGGCT GCTGAGAGTG 
CAGGTGCGCG TGCAGGACGA GAACGAGCAC GCGCCGGCCT TCGCGCGCGA CCCGCTGGCG 
TTGGCGCTCC CAGAGAACCC GGAGCCGGGC GCGACCTTGT ACACTTTCCG CGCGTCCGAC 
GCCGACGGCC CCGGCCCCAA CAGCGATGTG CGCTACCGCC TGCTGCGCCA AGAGCCGCCT 
GTGCCCGCGC TTCGCCTGGA CGCGCGCACC GGAGCGCTCA GCGCCCCGCG AGGCCTGGAT 
CGGGAGACCA CTCCGGCTCT GCTGCTGCTC GTGGAAGCCA CTGACCGGCC CGCCAACGCC 
AGCCGCCGCC GCACAGCACG CGTTTCCGCG CGCGTCTTTG TCACGGATGA GAATGACAAT 
GCTCCAGTCT TTGCCTCGCC CTCACGTGTG CGCCTCCCTG AGGATCACCC GCCCGGGCCC 
ACGGCGCTGC ACGTGGTAGC CCGGGACCCC GACCTGGGCG AGGCCGCACG GGTGTCCTAT 
CGCCTGGCAG CTGGCGCGGA CGGCTGTTTC CGGCTACACC CCAGCACCGG AGCGCTGTCC 
GTGGTGCGGT CTCTGGACCG CGAACAGCGA GCTGAGTACG TGCTGACTGT GGTGGCCTCT 
GACCACGGCT CCCCACCGCG CTCGGCCACG CAGCTTCTGA CTGTCAGTGT CGCCGACGTC 
AACGACGAGG CACCCGCTTT CCAACAGCAG GAGTACAGCG TCCTCTTGCG GGAGAACAGC 
CCGCCTGGAA CGTCTCTGCT CACTCTGCAG GCAACCGACC CCGATCTGGG GGCCAATGGG 
CAAGTGACTT ATGGAGGCAT CTCTGGCGAA AACTTCTCCC TGGATCCAGA CACCGGGGTC 
CTTACCACTC TTCGGGCTCT GGATCGGGAG GAGCAGGAGG AGATCAACTT GACAGTATAT 
GCCCGGGACG GGGGCTCGCC TCCACTGTTG ACCCACGTCA CAGTGCGAGT GGCTGTGGAG 
GATGAGAATG ACCATGCCCC AACCTTTGGG AGTGCCCATC TCTCCCTGGA GGTGCCCGAG 
GGCCAGGACC CCCAGACCCT TACCACGCTG CGGGCCTCTG ACCCGGATGT GGGAGCCAAC 
GGGCAGCTGC AGTACCGCAT CCTGGATGGG GACCCCTCAG GAGCCTTTGT CCTGGACCTA 
GCTTCTGGGG AGTTTGGCAC CATGAGGCCA CTAGACCGGG AGGTGGAGCC AGCATTCCAG 
CTGCAGATAG AAGCCCGGGA TGGAGGCCAG CCAGCTCTCA GCGCTACGCT TCTTGTGACA 
GTGACAGTGC TAGATGCCAA TGACCATGCC CCAGCCTTCC CTGTGCCCTC CTACTCTGTG 
GAGGTGCCTG AGGACGCACC TGCAGGGACC CTGCTGCTGC AGCTGCAGGC TCATGATCCT 
GATGCTGGGG CCAATGGTCG TGTGACCTAC TACCTGGGTA CAGGTGCAGC AGGAGCCTTC 
CTGCTAGAGC CCAGCTCTGG GGAATTGCGC ACAGCCACCA CCCTTGACAG AGAGCAGTGT 
CCCAGCTATG CCTTTACAGT GAATGCAGTG GACAGTGCAG CTGCTGGGCC CCTAAGCACC 
ACAGTGCCCG TCACCATCAC AGTGCGTGAT GTCAATGACC ACGCGCCCAC CTTCCCCACC 
AGTCCCCTGA GGCTGCGCCT GCCCCGCCCA GGCCCTGTCT TCAGCACTCC AGTTCTGCCT 
CTGGCCACTC TGCGAGCTGA AGACCGTGAC ACTGGTGCCA ATGCCTCCAT CCTGTACCGG 
CTGGCAGGCA CACCACCTCC TGGCACCACC GTGGACTCTT ACACTGGTGA AATCCGTGTG 
TCCCGCTCCC CTGTATCTCT GAGCCCCCGA GATCGTGTCC TCTTCATTGT GGCAACTGAC 
CTTGGCCGTC CAGCTCGCTC TGCCACTGGT GTGGTCATTA TTGGGCTGCA AGGGGAGTCT 
GAGCATGGAC CCCGCTTCTC CCGAGCCAGT AGTGAAGCCA TGCTCCGTGA GAATGCACCC 
CCAGGGACTC CCGTTGTCTC CCCCAAGGCT ATCCATGCAG GGGGCTCGAG TGGACCCATC 
ACATACAGCA TTCTCAGTGG GAATGAGAAA GGGACATTCT CCATCCAGCC TAGTACAGGA 
GCCATCACAG TCCGCTCAGC AGAGGGGCTG GATTTTGAGG CAAACCCACG GCTGCGACTG 
GTGCTGCAGG CAGAGAGTGG AGGAGCCTAT GCCTTCTCTG TGCTGACCCT AACCCTGCAA 
GATGCCAATG ACAACGCGCC CCGCTTCCTG CAGCCCCACT ACGTGGCCTT CCTTCCAGAG 
TCCAGGCCCT TGGAGGGACC CCTGCTACAG GTGGAAGCAG ATGACCTGGA CCAAGGCCCC 
AGTGGACAGA TCTCCTACAG TCTGGCCGCA TCCCAGCCAG CCCGGGGATT GTTCCATGTA 
GACCCAGCCA CGGGCACTAT CACCACCACA GCTATCCTGG ACCGCGAGAT CTGGGCCGAA 
ACACGGCTGG TGCTGATGGC CACAGACAGA GGAAGCCCAG CCCTGGTGGG CTCAGCTACC 
CTGACGGTGA TGGTCATCGA CACCAATGAC AATCGCCCCA CCATCCCCCA GCCCTGGGAG 
CTCCGAGTGT CAGAAGATGC ACTGCTGGGC TCGGAGATCG CACAGGTAAC GGGGAATGAT 
GTGGACTCAG GACCAGTGCT GTGGTATGTT CTGAGCCCTT CTGGGCCCCA GGATCCCTTC 
AGTGTCGGCC GCTATGGAGG CCGCCTCTCC CTCACGGGCC CCTTGGATTT TGAGCAGCGC 
GACCGCTACC ACCTGCAGCT CCTGGCACAC GACGGGCCTC ACGAGGGCCA GGCCAACCTC 
ACGGTGTTCG TGGAGGATGT CAATGACAAC TCCCCTGCCT TCTCACAGAC TCTCTACCAG 
GTGATGCTGC TCGAGCACAC ACCCCCGGGC AGTGCCATTC TCTCCGTCTC GGCCACTGAC 
CGGGACTCGG GCGCCAACGG TCACGTCTCC TACCACCTGG CTTCCTCTGC TGAGGGCTTC 
AGCATTGACC CCAACAACGG GACCCTATTC ACAACAGTGG GGACAGTGGC CTTGGGCCAT 
GAAGGGCCAG GCATGGTGGA CGTGGTGCTG GAAGCCCGAG ACCACGGAGT GCCGGTTCGG 
GCCGCCCGAG CCACAGTGCA CGTGCAGCTG CAGGATCAGA ACGACCATGC CCCGAGCTTC 
ACGCTGCCAC ACTACCGCGT GGCTGTGACT GAGGATCTGC CGCCCGGCTC CACCCTGCTC 
ACCCTGGAGG CCACAGATGC TGACGGGAGC CGCAGCCATG CCACCGTGGA CTACAGCATT 
GTCAGTGGCA ACCGGGGCCG AGTCTTTCAG CTGGAACCCC GGCTGGCCGA GGCTGGGGAG 
AGTGATGGAC TAGGCCCCCG GGCCCTGGGC TGCCTGGTGT TGCTTGAGCC TCTAGACTTT 
GAAAGCCTAA CCCAATACAA CCTAACTGTG GCAGCAACTG ACCGGGGGCA GCCACCTCGA 
AGTTCGGCTG TGCCAGTCAC CGTCAGCGTG CTGGATGTCA ACGACAACCC ACCTGTCTTC 
ACCCGAGCAG CCTACCGTGT GGCGGTGCCT GAGGACACGC CTGTGGGAGC CGAGCTGCTG 
CACGTGGAGG CCTCCGACGC TGATCCAGGC CCCCATGGCC TTGTACGCTT CACCCTCAGC 
TCAGGCGACC CTCTGGGGCT CTTTGAGCTG GATGAGAGCT CCGGGGACTT GCGACTGGCC 
CGCCCGTTGG ACTGTGAGAC CCAAGCTCGA CATCAGCTTG TAGTGCAGGC TTCTGACCCT 
GCCGGGGTAC ACTTTGCTCT GGCACCAGTG ACCATTGAGG TCCAGGACGT GAATGACCAT 
GGCCCCGCCT TCCCTCTGAG CTTGCTCAGC ACCAGCCTGG CTGAGAACCA GCCTCCAGGC 
ACTCTTGTTA CCACCCTGCA TGCAATTGAT GGGGATGCTG GGGCTTTCGG AAGGCTCCAC 
TACAGCCTGC TGGAGGCTGG GCCGGGGCCC GAGGGCCGCG AGGCATTTGC GCTCAACAGC 
TCAACTGGGG AGTTGCGGGC ACGAGTGGCC TTTGACTACG AGCACACTGG CAGCTTCCAG 
CTGCTGGTGG GCGCTACAGA TGCCGGGAAC CTGTCCGCCT CAGTCACTGT GTCAGTGCTG 
GTGACCGGTG AGGATGAGTA TGACCCAGTG TTCCTGGCTC CAGCTTTCCA CTTTCAGGTG 
CCAGAAGGTG CACGGCGTGG CCACAGCCTG GGTCACGTGC AGGCCACAGA CGAGGATGGA 
GGTGCTGACG GCCTGGTGCT GTACTCGCTG GCCGCCTCGT CCCCCTATTT TGGTATCAAC 
CAGACTACAG GTGCCCTGTA CCTGCGGGTG GACAGCCGGG CACCAGGCAG TGGAACAGGC 
ACCTCCGGGG GTGGGGGCCG GACCCGACGT GAGGCGCCAC GGGAGCTGAG GCTGGAGGTG 
GTGGCAAGGG GGCCTCTGCC CGGTTCTCGG AGTGCCACCG TGCCTGTGAC TGTGGACATC 
ACCCACACTG CGCTGGGCCT GGCACCTGAC CTCAACCTGC TCTTGGTGGG AGCCGTGGCA 
GCCTCTCTGG GAGTCGTGGT GGTGCTTGCC CTGGCAGCCC TTGTCCTGGG ACTGGTGCGG 
GCCCGGAGCC GCAAAGCTGA GGCGGCGCCT GGCCCGATGT CCCAGGCGGC ACCCCTGGCC 
AGTGGCTCAC TGCAGAAGCT GGGCCGAGAG CCACCCAGCC CGCCGCCCTC AGAGCACCTG 
TATCACCAGA CCCTCCCCAG CTATGGTGGG CCTGGAGCTG GAGGACCCTA CCCCCGCGGT 
GGCTCCCTGG ACCCTTCACA CTCAAGCGGC CGAGGCTCAG CAGAGGCTGC GGAAGATGAC 
GAGATCCGCA TGATCAACGA GTTCCCCCGA GTGGCCAGTG TGGCCTCCTC CCTGGCTGCC 
CGCGGCCCTG ACTCCGGCAT CCAGCAGGAT GCAGACGGAC TGAGTGACAC CTCCTGTGAG 
CCACCTGCCC CTGACACCTG GTATAAGGGC CGTAAGGCAG GGCTGCTGCT GCCGGGTGCA 
GGAGCCACTC TGTACAGAGA AGACGGCCCC CCAGCCACTG CCACCACCTT CCTGGGGGGC 
TGTGGCCTAA GCCCCGCGCC CACCGGGGAC TACGGCTTTC CAGCAGATGG CAAGCCCTGT 
GTGGCGGGAG CGCTGACGGC CATCGTGGCC GGTGAGGAGG AGCTCCGTGG CAGCTACAAC 
TGGGACTACC TGCTGAGCTG GTGCCCTCAG TTCCAGCCGC TGGCCAGTGT CTTCACAGAG 
ATTGCCCGGC TCAAGGATGA AGCTCGGCCA TGTCCCCCGG CGCCCCGTAT TGACCCACCG 
CCCCTCATCA CTGCCGTGGC CCACCCAGGA GCCAAGTCTG TGCCCCCCAA ACCAGCCAGC 
ACGGCCGCAA CCCGGGCCGT CTTCCCACCA GCCTCTCACC GCTCCCCCAT CAGCCACGAA 
GGTTCCCTGT CCTCGGCGGC CATGTCTCCC AGCTTCTCAC CTTCACTATC TCCTCTGGCC 
GCTCGCTCAC CCGTCGTCTC ACCGTTTGGG GTGGCCCAGG GCCCCTCAGC CTCAGCTCTG 
AGCGCAGAGT CTGGCCTGGA GCCACCGGAC GACACCGAGC TCCATATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCHS1 ( XM_002693149.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002693195.1 Bos taurus dachsous cadherin-related 1 (DCHS1), mRNA.

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CloneID	OBa26442
Clone ID Related Accession (Same CDS sequence)	XM_002693149.4 , XM_002701345.4
Accession Version	XM_002693149.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protocadherin-16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104411.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002693149.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG AGTGGGCCAC TGCACCTTCG TGCCCGGGCA TGCAGATCCC CAGGCCTCTC 
CCCCTGCTGT CATTGCTGAT GCTGCTGCTC CTGGGGGCGG GGGCTCCAGG TGCCTGGGGT 
CAGGCTGGGA GCTTGGACCT GCAGATTGAT GAGGAGCAGC CAGCGGGCAC GCTGATCGGG 
GACATCAGTG CAGGGCTTCC AGCGGGCACA GCAGCACCTC CCATGTACTT CATCTCTGCC 
CAGGAGGGCA GCGGGGTGGG CACGGACCTG GCCATCGATG AACACAGTGG GGTGGTCCGT 
ACAGCCCGTG TCTTGGACCG AGAGCGGCGG GATCGCTACC GCTTCACTGC AGTCACTCCC 
GACGGTGCCA CTGTGGAAGT TACCGTGCGA GTGGCTGACA TCAATGACCA TGCTCCGGCT 
TTCCCACAGG CTCGGGCTGT CCTGCAGATA CCTGAGCATA CAGCACTTGG CACCCGCTAC 
CCACTGGAGC CTGCTCGTGA TGCCGACGCT GGCCGCCTGG GAACGCAGGG CTATGCCCTG 
TCTGGTGATG GGGCTGGAGA GACCTTCCGG CTGGAGACAC GCCCTGGTCC AGATGGGGCC 
CCAGTGCCTG AGCTGGTAGT TACCGGAGAG CTGGACCGAG AGAACCGCTC ACACTACATG 
CTGCAACTGG AGGCCTATGA CGGTGGTTCA CCCCCTCGGA GGGCCCAGGC CCTGCTGGAC 
GTGACACTGC TAGACATCAA TGACCATGCC CCAGCTTTCA ATCAGAGCCG CTACCACGCT 
GCTGTGTCCG AGAGCCTGGC CCCCGGCAGT CCTGTCTTGC AGGTGTATGC CTCTGATGCC 
GATGCAGGTG CCAATGGGGC TGTAACTTAT GAGATCAACC GGAGGCAGAG CGAGGGTGAT 
GGACCCTTCT CCATTGATGC ACACACGGGG CTGCTGCGGC TGGAGCGGCC GCTGGACTTT 
GAGCAACGTC GGGTCCACGA ACTGGTGGTG CAGGCTCGGG ATGGAGGGGC TCACCCTGAG 
CTGGGCTCAG CCTTTGTGAC CGTGCATGTG CGAGATGCCA ATGACAATCA GCCCTCCATG 
ACTGTCATCT TCCTGAGTGC AGATGGCTCC CCCCGTGTAT CTGAGGCTGC CCCCCCGGGC 
CAGCTTGTTG CTCGAATCTC TGTGTCAGAC CCAGATGACG GTGACTTTGC GCATGTCAAC 
GTGTCCCTGG AGGGTGGAGA GGGCCACTTT GCGCTCAGCA CCCAGGACAG CGTCATCTAC 
CTGGTGTGTG TGGCTCGGAG GCTGGATCGG GAGGAGCGCG ACGCCTACAA CTTGCGAGTT 
ACTGCCACAG ACTCAGGCTC ACCCCCACTG CGGGCCGAGG CTGCCTTTGT GCTACACGTC 
ACTGATGTCA ATGACAATGC GCCTGCCTTT GACCGCCAGC TCTACCGACC CGAGCCTCTG 
CCTGAGGTTG CCCTGCCTGG CAGCTTCGTG GCGCGGGTGA CGGCCCGGGA TCCTGACCAG 
GGCACCAATG GTCAGGTCAC CTACAGCCTG GCCCCAGGCG CCCACACCCG CTGGTTCTCC 
ATAGACCCCA CCTCAGGCAT TGTCACTACA GCTGCTTTGC TGGACTATGA GTTGGAACCT 
CAGCCACAGC TAATCGTGGT GGCCACGGAT GGGGGCTTGC CCCCTCTCTC CTCCTCTGCC 
ACAGTGAGCG TGGTCTTGCA AGATGTGAAT GATAACGAGC CGCAGTTCCA GAGGACTTTC 
TACAACGCCT CGCTGCCTGA GGGCACTCAG CCAGGAACCT GCTTCCTGCA GGTGACAGCC 
ACAGATGCAG ACAGTGGCCC ATTTGGCCTC CTCTCCTATT CCTTGGGTGC CGGACTTGGG 
ACCTCAGGGT CTCCCCCGTT CCGCATCGAT GCCCACAGTG GTGACGTGTG TACGACCCGG 
ACCCTGGACC GCGATCAGGG GCCCTCAAGC TTTGACTTCA CAGTGACAGC TGTGGATGGG 
GGAGGCCTCA AGTCTATGGT ATATGTGAAG GTGTTTGTGT CAGACGAGAA TGACAACCCA 
CCTCAGTTTT ATCCACGGGA GTATGCTGCC AGTCTGAGCG CCCAGAGTGC ACCAGGCACA 
GCTGTGCTGA GGGTACGTGC CCATGACCCT GATCAGGGAC CCCATGGGCG ACTCTCCTAC 
CACATCCTGG CTGGCAACAG CCCCCCGCTC TTTGCCTTGG ACGAGCACTC AGGACTATTG 
ACAGTAGCCT GGCCCTTGGC CAGACGGGCC AGTTCCATGG TGCAGCTGGA GATCGGGGCT 
CGGGATGGAG GTGGCCTGCA GGCGGAGCCC AATGCCCGAG TCAACATCAG CATTGTGCCT 
GGAACTCCTG TACCACCAGT ATTTGAGCAG CTTCAGTATG TCTTTTCTGT GCCAGAGGAT 
GTGGCACCAG GCACCAGTGT GGGCGTAGTC CAGGCACACA ATCCACCAGG TCGCTTGGGA 
CCGGTGACCC TTGCCCTATC GGGGGGCGAT CCCCGAGGAC TCTTCTCCCT AGATGGGGCC 
ACTGGGCTGT TACAGACACT TCGCCCTCTG GACCGGGAGC TGCTGGGACC AGTGCTGGAG 
CTGGAGGTGC GGGCAGGCAG CGGAGTGCCA CCGGCTTTCG CTGTAGCTCG GGTGCGCGTG 
CTGCTGGACG ATGTGAATGA CAACTCCCCT GCCTTCCCTG CGCCTGAAGA CACAGTGCTG 
CTGCCATCGA GCACTGCCCC AGGGACCCTC ATCTATACAC TGCGGGCTCT GGACCCCGAC 
TCAGGAGTTA ACAGTCGAGT CACCTTTACC CTGCTTGCTG GGGGTGGTGG GACCTTCACT 
GTGGACCCCA CCACAGGCCA CATACGGCTT ATGGGACCTC TGGGGCCTCC GGGGGGGCCA 
GCCCATGAAC TGGAGCTGGA AGCCCGGGAT GGAGGCTCCC CACCTCGCAC CAGCCACTTT 
CGACTGCGGG TGGTGGTACA GGACTTGGGG ACCCGTGGGC TGGCTCCCCA CTTCGACAGC 
CCTACCTACC GTGTAGACCT GCCCTCAGGC ACCACCCCTG GAACTCAAGT CCTACAAGTG 
CAGGCTCGAG CACCAGATGG GAGCCCTGTC ACCTACCACC TTGCAGCAGA TGGGCCAGGT 
AGCCCCTTTG GCCTGGAACC ACAGAGTGGA TGGCTGTGGG TGCGGGCAGC ACTGGACCAT 
GAGGCCCAGG AGTTGTACAC ACTGAAAGTA ATGGCAGTGT CTGGATCCAA AGCTGAGTTG 
GGGCAGCAGA CAGCCACGGC CACCGTGAGG ATCAGCATCC TCAACCAGAA CGACCACAGT 
CCCCGCTTGT CTGAGGAGCC CACCTTCCTG GCTGTGGCTG AGAACCAGCC CCCAGGGACA 
AGCGTGGGTC GGGTCTTTGC CACTGACCGA GATTCAGGAA TCAACGGACG TCTGACCTAC 
AGCCTGCGAC AGCTGTCAGA AGACAGCAAG GCCTTCCGCA TCCACCCCCA GACTGGAGAG 
GTGACCACAC TACAAACTCT GGACCGTGAG CGGCAGAACA GCTACCAGCT TCTGGTGCAG 
GTTCAGGATG CAGGGAGCCC ACCCCGCAGC ACCACAGGCA CCGTGCACAT CGCAGTGCTT 
GACCTCAATG ACAACAGCCC TACCTTCCTG CAGGCCTCAG GGGCTGCTGG TGGAGGCCTC 
CCCATACAGG TACCAGATGG TGTGCCTCCA GGAACATTGG TGACAACCCT GCAGGCCAAG 
GATCCAGATG AGGGGGAGAA TGGAACCATC CTGTACATGC TAACTGGTCC TGGCTCAGAG 
CTCTTCTCCC TGCACCCTCA CTCGGGAGAG CTGCTCACGG CAGCACCCCT GATCCGAGCA 
GAGCGGCCAC ACTACGTGCT CACATTGAGC GCTCATGACC AAGGCAGCCC CCCTCGGAGC 
TCCAGCCTCC AGCTGCTAGT GCAGGTGCTT CCCTCCACTC GCTCGGCTGA GCCTGCACCG 
GAACCCTCAG AGCCAGACCC GGTGGCGCCT GTGCCTGTCG TGCTGACCGT GACAGCAGCC 
GAGGGGCTCC GGCCCGGCTC CCTGTTGGGC TCTGTGGCAC CGCTGGAGCA GGCGGGGGTG 
GGCGCCCTTA CCTACACACT GGTGGGCGGC GCCGACCCAG AGGGCACCTT TGCGCTGGAC 
GCAGCCTCTG GGCGCTTGTA CCTGGCGCGG CCCCTGGACT TCGAGGCGGG TCCGGCTTGG 
CGCGCGCTCA CGGTGCGCGC CGAGGGGCCG GGAGGCGCGG GCGCGCGGCT GCTGAGAGTG 
CAGGTGCGCG TGCAGGACGA GAACGAGCAC GCGCCGGCCT TCGCGCGCGA CCCGCTGGCG 
TTGGCGCTCC CAGAGAACCC GGAGCCGGGC GCGACCTTGT ACACTTTCCG CGCGTCCGAC 
GCCGACGGCC CCGGCCCCAA CAGCGATGTG CGCTACCGCC TGCTGCGCCA AGAGCCGCCT 
GTGCCCGCGC TTCGCCTGGA CGCGCGCACC GGAGCGCTCA GCGCCCCGCG AGGCCTGGAT 
CGGGAGACCA CTCCGGCTCT GCTGCTGCTC GTGGAAGCCA CTGACCGGCC CGCCAACGCC 
AGCCGCCGCC GCACAGCACG CGTTTCCGCG CGCGTCTTTG TCACGGATGA GAATGACAAT 
GCTCCAGTCT TTGCCTCGCC CTCACGTGTG CGCCTCCCTG AGGATCACCC GCCCGGGCCC 
ACGGCGCTGC ACGTGGTAGC CCGGGACCCC GACCTGGGCG AGGCCGCACG GGTGTCCTAT 
CGCCTGGCAG CTGGCGCGGA CGGCTGTTTC CGGCTACACC CCAGCACCGG AGCGCTGTCC 
GTGGTGCGGT CTCTGGACCG CGAACAGCGA GCTGAGTACG TGCTGACTGT GGTGGCCTCT 
GACCACGGCT CCCCACCGCG CTCGGCCACG CAGCTTCTGA CTGTCAGTGT CGCCGACGTC 
AACGACGAGG CACCCGCTTT CCAACAGCAG GAGTACAGCG TCCTCTTGCG GGAGAACAGC 
CCGCCTGGAA CGTCTCTGCT CACTCTGCAG GCAACCGACC CCGATCTGGG GGCCAATGGG 
CAAGTGACTT ATGGAGGCAT CTCTGGCGAA AACTTCTCCC TGGATCCAGA CACCGGGGTC 
CTTACCACTC TTCGGGCTCT GGATCGGGAG GAGCAGGAGG AGATCAACTT GACAGTATAT 
GCCCGGGACG GGGGCTCGCC TCCACTGTTG ACCCACGTCA CAGTGCGAGT GGCTGTGGAG 
GATGAGAATG ACCATGCCCC AACCTTTGGG AGTGCCCATC TCTCCCTGGA GGTGCCCGAG 
GGCCAGGACC CCCAGACCCT TACCACGCTG CGGGCCTCTG ACCCGGATGT GGGAGCCAAC 
GGGCAGCTGC AGTACCGCAT CCTGGATGGG GACCCCTCAG GAGCCTTTGT CCTGGACCTA 
GCTTCTGGGG AGTTTGGCAC CATGAGGCCA CTAGACCGGG AGGTGGAGCC AGCATTCCAG 
CTGCAGATAG AAGCCCGGGA TGGAGGCCAG CCAGCTCTCA GCGCTACGCT TCTTGTGACA 
GTGACAGTGC TAGATGCCAA TGACCATGCC CCAGCCTTCC CTGTGCCCTC CTACTCTGTG 
GAGGTGCCTG AGGACGCACC TGCAGGGACC CTGCTGCTGC AGCTGCAGGC TCATGATCCT 
GATGCTGGGG CCAATGGTCG TGTGACCTAC TACCTGGGTA CAGGTGCAGC AGGAGCCTTC 
CTGCTAGAGC CCAGCTCTGG GGAATTGCGC ACAGCCACCA CCCTTGACAG AGAGCAGTGT 
CCCAGCTATG CCTTTACAGT GAATGCAGTG GACAGTGCAG CTGCTGGGCC CCTAAGCACC 
ACAGTGCCCG TCACCATCAC AGTGCGTGAT GTCAATGACC ACGCGCCCAC CTTCCCCACC 
AGTCCCCTGA GGCTGCGCCT GCCCCGCCCA GGCCCTGTCT TCAGCACTCC AGTTCTGCCT 
CTGGCCACTC TGCGAGCTGA AGACCGTGAC GCTGGTGCCA ATGCCTCCAT CCTGTACCGG 
CTGGCAGGCA CACCACCTCC TGGCACCACC GTGGACTCTT ACACTGGTGA AATCCGTGTG 
TCCCGCTCCC CTGTATCTCT GAGCCCCCGA GATCGTGTCC TCTTCATTGT GGCAACTGAC 
CTTGGCCGTC CAGCTCGCTC TGCCACTGGT GTGGTCATTA TTGGGCTGCA AGGGGAGTCT 
GAGCATGGAC CCCGCTTCTC CCGAGCCAGT AGTGAAGCCA TGCTCCGTGA GAATGCACCC 
CCAGGGACTC CCGTTGTCTC CCCCAAGGCT ATCCATGCAG GGGGCTCAAG TGGACCCATC 
ACATACAGCA TTCTCAGTGG GAATGAGAAA GGGACATTCT CCATCCAGCC TAGTACAGGA 
GCCATCACAG TCCGCTCAGC AGAGGGGCTG GATTTTGAGG CAAACCCACG GCTGCGACTG 
GTGCTGCAGG CAGAGAGTGG AGGAGCCTAT GCCTTCTCTG TGCTGACCCT AACCCTGCAA 
GATGCCAATG ACAACGCGCC CCGCTTCCTG CAGCCCCACT ACGTGGCCTT CCTTCCAGAG 
TCCAGGCCCT TGGAGGGACC CCTGCTACAG GTGGAAGCAG ATGACCTGGA CCAAGGCCCC 
AGTGGACAGA TCTCCTACAG TCTGGCCGCA TCCCAGCCAG CCCGGGGATT GTTCCATGTA 
GACCCAGCCA CGGGCACTAT CACCACCACA GCTATCCTGG ACCGCGAGAT CTGGGCCGAA 
ACACGGCTGG TGCTGATGGC CACAGACAGA GGAAGCCCAG CCCTGGTGGG CTCAGCTACC 
CTGACGGTGA TGGTCATCGA CACCAATGAC AATCGCCCCA CCATCCCCCA GCCCTGGGAG 
CTCCGAGTGT CAGAAGATGC ACTGCTGGGC TCGGAGATCG CACAGGTAAC GGGGAATGAT 
GTGGACTCAG GACCAGTGCT GTGGTACGTT CTGAGCCCTT CTGGGCCCCA GGATCCCTTC 
AGTGTCGGCC GCTATGGAGG CCGCCTCTCC CTCACGGGCC CCTTGGATTT TGAGCAGCGC 
GACCGCTACC ACCTGCAGCT CCTGGCACAC GACGGGCCTC ACGAGGGCCG GGCCAACCTC 
ACGGTGTTCG TGGAGGATGT CAATGACAAC TCCCCTGCCT TCTCACAGAC TCTCTACCAG 
GTGATGCTGC TCGAGCACAC ACCCCCGGGC AGTGCCATTC TCTCCGTCTC GGCCACTGAC 
CGGGACTCGG GCGCCAACGG TCACGTCTCC TACCACCTGG CTTCCTCTGC TGAGGGCTTC 
AGCATTGACC CCAACAACGG GACCCTATTC ACAACAGTGG GGACAGTGGC CTTGGGCCAT 
GAAGGGCCAG GCATGGTGGA CGTGGTGCTG GAAGCCCGAG ACCACGGAGT GCCGGTTCGG 
GCCGCCCGAG CCACAGTGCA CGTGCAGCTG CAGGATCAGA ACGACCATGC CCCGAGCTTC 
ACGCTGCCAC ACTACCGCGT GGCTGTGACT GAGGATCTGC CGCCCGGCTC CACCCTGCTC 
ACCCTGGAGG CCACAGATGC CGACGGGAGC CGCAGCCATG CCGCCGTGGA CTACAGCATT 
GTCAGTGGCA ACCGGGGCCG AGTCTTTCAG CTGGAACCCC GGCTGGCGGA GGCTGGGGAG 
AGTGATGGAC TAGGCCCCCG GGCCCTGGGC TGCCTGGTGT TGCTTGAGCC TCTAGACTTT 
GAAAGCCTAA CCCAGTACAA CCTAACTGTG GCAGCAACTG ACCGGGGGCA GCCACCTCGA 
AGTTCGGCTG TGCCAGTCAC CGTCAGCGTG CTGGATGTCA ACGACAACCC ACCTGTCTTC 
ACCCGAGCAG CCTACCGTGT GGCGGTGCCT GAGGACACGC CTGTGGGAGC CGAGCTGCTG 
CACGTGGAGG CCTCCGACGC TGATCCAGGC CCCCATGGCC TTGTACGCTT CACCCTCAGC 
TCAGGCGACC CTCTGGGGCT CTTTGAGCTG GATGAGAGCT CCGGGGACTT GCGACTGGCC 
CGCCCGTTGG ACTGTGAGAC CCAAGCTCGA CATCAGCTTG TAGTGCAGGC TGCCGACCCT 
GCCGGGGCAC ACTTTGCTCT GGCACCAGTG ACCATTGAGG TCCAGGACGT GAATGACCAT 
GGCCCCGCCT TCCCTCTGAG CTTGCTCAGC ACCAGCCTGG CTGAGAACCA GCCTCCAGGC 
ACTCTTGTTA CCACCCTGCA TGCAATTGAT GGGGATGCTG GGGCTTTCGG AAGGCTCCAC 
TACAGCCTGC TGGAGGCTGG GCCGGGGCCC GAGGGCCGCG AGGCGTTTGC GCTCAACAGC 
TCAACTGGGG AGTTGCGGGC ACGAGTGGCC TTTGACTACG AGCACACTGG CAGCTTCCAG 
CTGCTGGTGG GCGCTACGGA TGCCGGGAAC CTGTCCGCCT CAGTCACTGT GTCAGTGCTG 
GTGACCGGTG AGGATGAGTA TGACCCAGTG TTCCTGGCTC CAGCTTTCCA CTTTCAGGTG 
CCAGAAGGTG CACGGCGTGG CCACAGCCTG GGTCACGTGC AGGCCACAGA CGAGGATGGA 
GGTGCTGACG GCCTGGTGCT GTACTCGCTG GCCGCCTCGT CCCCCTATTT TGGTATCAAC 
CAGACTACAG GTGCCCTGTA CCTGCGGGTG GACAGCCGGG CACCAGGCAG TGGAACAGGC 
ACCTCCGGGG GTGGGGGCCG GACCCGACGT GAGGCGCCAC GGGAGCTGAG GCTGGAGGTG 
GTGGCAAGGG GGCCTCTGCC CGGTTCTCGG AGTGCCACCG TGCCTGTGAC TGTGGACATC 
ACCCACACTG CGCTGGGCCT GGCACCTGAC CTCAACCTGC TCTTGGTGGG AGCCGTGGCA 
GCCTCTCTGG GAGTCGTGGT GGTGCTTGCC CTGGCAGCCC TTGTCCTGGG ACTGGTGCGG 
GCCCGGAGCC GCAAAGCTGA GGCGGCGCCT GGCCCGATGT CCCAGGCGGC ACCCCTGGCC 
AGTGGCTCAC TGCAGAAGCT GGGCCGAGAG CCACCCAGCC CGCCGCCCTC AGAGCACCTG 
TATCACCAGA CCCTCCCCAG CTATGGTGGG CCTGGAGCTG GAGGACCCTA CCCCCGCGGT 
GGCTCCCTGG ACCCTTCACA CTCAAGCGGC CGAGGCTCAG CAGAGGCTGC GGAAGATGAC 
GAGATCCGCA TGATCAACGA GTTCCCCCGC GTGGCCAGTG TGGCCTCCTC CCTGGCTGCC 
CGCGGCCCTG ACTCCGGCAT CCAGCAGGAT GCAGACGGAC TGAGTGACAC CTCCTGTGAG 
CCACCTGCCC CTGACACCTG GTATAAGGGC CGTAAGGCAG GGCTGCTGCT GCCGGGTGCA 
GGAGCCACTC TGTACAGAGA AGACGGCCCC CCAGCCACTG CCACCACCTT CCTGGGGGGC 
TGTGGCCTAA GCCCCGCGCC CACCGGGGAC TACGGCTTTC CAGCAGATGG CAAGCCCTGT 
GTGGCGGGAG CGCTGACGGC CATCGTGGCC GGTGAGGAGG AGCTCCGTGG CAGCTACAAC 
TGGGACTACC TGCTGAGCTG GTGCCCTCAG TTCCAGCCGC TGGCCAGTGT CTTCACAGAG 
ATTGCCCGGC TCAAGGATGA AGCTCGGCCG TGTCCCCCGG CGCCCCGTAT TGACCCACCG 
CCCCTCATCA CTGCCGTGGC CCACCCAGGA GCCAAGTCTG TGCCCCCCAA ACCAGCCAGC 
ACGGCCGCAA CCCGGGCCGT CTTCCCACCA GCCTCTCACC GCTCCCCCAT CAGCCACGAA 
GGTTCCCTGT CCTCGGCGGC CATGTCTCCC AGCTTCTCAC CTTCACTATC TCCTCTGGCC 
GCTCGCTCAC CCGTCGTCTC ACCATTTGGG GTGGCCCAGG GCCCCTCAGC CTCAGCTCTG 
AGCGCAGAGT CTGGCCTGGA GCCACCAGAC GACACCGAGC TCCATATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002693195.1
CDS	242..10132
Translation	MQKEWATAPSCPGMQIPRPLPLLSLLMLLLLGAGAPGAWGQAGSLDLQIDEEQPAGTLIGDISAGLPAGTAAPPMYFISAQEGSGVGTDLAIDEHSGVVRTARVLDRERRDRYRFTAVTPDGATVEVTVRVADINDHAPAFPQARAVLQIPEHTALGTRYPLEPARDADAGRLGTQGYALSGDGAGETFRLETRPGPDGAPVPELVVTGELDRENRSHYMLQLEAYDGGSPPRRAQALLDVTLLDINDHAPAFNQSRYHAAVSESLAPGSPVLQVYASDADAGANGAVTYEINRRQSEGDGPFSIDAHTGLLRLERPLDFEQRRVHELVVQARDGGAHPELGSAFVTVHVRDANDNQPSMTVIFLSADGSPRVSEAAPPGQLVARISVSDPDDGDFAHVNVSLEGGEGHFALSTQDSVIYLVCVARRLDREERDAYNLRVTATDSGSPPLRAEAAFVLHVTDVNDNAPAFDRQLYRPEPLPEVALPGSFVARVTARDPDQGTNGQVTYSLAPGAHTRWFSIDPTSGIVTTAALLDYELEPQPQLIVVATDGGLPPLSSSATVSVVLQDVNDNEPQFQRTFYNASLPEGTQPGTCFLQVTATDADSGPFGLLSYSLGAGLGTSGSPPFRIDAHSGDVCTTRTLDRDQGPSSFDFTVTAVDGGGLKSMVYVKVFVSDENDNPPQFYPREYAASLSAQSAPGTAVLRVRAHDPDQGPHGRLSYHILAGNSPPLFALDEHSGLLTVAWPLARRASSMVQLEIGARDGGGLQAEPNARVNISIVPGTPVPPVFEQLQYVFSVPEDVAPGTSVGVVQAHNPPGRLGPVTLALSGGDPRGLFSLDGATGLLQTLRPLDRELLGPVLELEVRAGSGVPPAFAVARVRVLLDDVNDNSPAFPAPEDTVLLPSSTAPGTLIYTLRALDPDSGVNSRVTFTLLAGGGGTFTVDPTTGHIRLMGPLGPPGGPAHELELEARDGGSPPRTSHFRLRVVVQDLGTRGLAPHFDSPTYRVDLPSGTTPGTQVLQVQARAPDGSPVTYHLAADGPGSPFGLEPQSGWLWVRAALDHEAQELYTLKVMAVSGSKAELGQQTATATVRISILNQNDHSPRLSEEPTFLAVAENQPPGTSVGRVFATDRDSGINGRLTYSLRQLSEDSKAFRIHPQTGEVTTLQTLDRERQNSYQLLVQVQDAGSPPRSTTGTVHIAVLDLNDNSPTFLQASGAAGGGLPIQVPDGVPPGTLVTTLQAKDPDEGENGTILYMLTGPGSELFSLHPHSGELLTAAPLIRAERPHYVLTLSAHDQGSPPRSSSLQLLVQVLPSTRSAEPAPEPSEPDPVAPVPVVLTVTAAEGLRPGSLLGSVAPLEQAGVGALTYTLVGGADPEGTFALDAASGRLYLARPLDFEAGPAWRALTVRAEGPGGAGARLLRVQVRVQDENEHAPAFARDPLALALPENPEPGATLYTFRASDADGPGPNSDVRYRLLRQEPPVPALRLDARTGALSAPRGLDRETTPALLLLVEATDRPANASRRRTARVSARVFVTDENDNAPVFASPSRVRLPEDHPPGPTALHVVARDPDLGEAARVSYRLAAGADGCFRLHPSTGALSVVRSLDREQRAEYVLTVVASDHGSPPRSATQLLTVSVADVNDEAPAFQQQEYSVLLRENSPPGTSLLTLQATDPDLGANGQVTYGGISGENFSLDPDTGVLTTLRALDREEQEEINLTVYARDGGSPPLLTHVTVRVAVEDENDHAPTFGSAHLSLEVPEGQDPQTLTTLRASDPDVGANGQLQYRILDGDPSGAFVLDLASGEFGTMRPLDREVEPAFQLQIEARDGGQPALSATLLVTVTVLDANDHAPAFPVPSYSVEVPEDAPAGTLLLQLQAHDPDAGANGRVTYYLGTGAAGAFLLEPSSGELRTATTLDREQCPSYAFTVNAVDSAAAGPLSTTVPVTITVRDVNDHAPTFPTSPLRLRLPRPGPVFSTPVLPLATLRAEDRDAGANASILYRLAGTPPPGTTVDSYTGEIRVSRSPVSLSPRDRVLFIVATDLGRPARSATGVVIIGLQGESEHGPRFSRASSEAMLRENAPPGTPVVSPKAIHAGGSSGPITYSILSGNEKGTFSIQPSTGAITVRSAEGLDFEANPRLRLVLQAESGGAYAFSVLTLTLQDANDNAPRFLQPHYVAFLPESRPLEGPLLQVEADDLDQGPSGQISYSLAASQPARGLFHVDPATGTITTTAILDREIWAETRLVLMATDRGSPALVGSATLTVMVIDTNDNRPTIPQPWELRVSEDALLGSEIAQVTGNDVDSGPVLWYVLSPSGPQDPFSVGRYGGRLSLTGPLDFEQRDRYHLQLLAHDGPHEGRANLTVFVEDVNDNSPAFSQTLYQVMLLEHTPPGSAILSVSATDRDSGANGHVSYHLASSAEGFSIDPNNGTLFTTVGTVALGHEGPGMVDVVLEARDHGVPVRAARATVHVQLQDQNDHAPSFTLPHYRVAVTEDLPPGSTLLTLEATDADGSRSHAAVDYSIVSGNRGRVFQLEPRLAEAGESDGLGPRALGCLVLLEPLDFESLTQYNLTVAATDRGQPPRSSAVPVTVSVLDVNDNPPVFTRAAYRVAVPEDTPVGAELLHVEASDADPGPHGLVRFTLSSGDPLGLFELDESSGDLRLARPLDCETQARHQLVVQAADPAGAHFALAPVTIEVQDVNDHGPAFPLSLLSTSLAENQPPGTLVTTLHAIDGDAGAFGRLHYSLLEAGPGPEGREAFALNSSTGELRARVAFDYEHTGSFQLLVGATDAGNLSASVTVSVLVTGEDEYDPVFLAPAFHFQVPEGARRGHSLGHVQATDEDGGADGLVLYSLAASSPYFGINQTTGALYLRVDSRAPGSGTGTSGGGGRTRREAPRELRLEVVARGPLPGSRSATVPVTVDITHTALGLAPDLNLLLVGAVAASLGVVVVLALAALVLGLVRARSRKAEAAPGPMSQAAPLASGSLQKLGREPPSPPPSEHLYHQTLPSYGGPGAGGPYPRGGSLDPSHSSGRGSAEAAEDDEIRMINEFPRVASVASSLAARGPDSGIQQDADGLSDTSCEPPAPDTWYKGRKAGLLLPGAGATLYREDGPPATATTFLGGCGLSPAPTGDYGFPADGKPCVAGALTAIVAGEEELRGSYNWDYLLSWCPQFQPLASVFTEIARLKDEARPCPPAPRIDPPPLITAVAHPGAKSVPPKPASTAATRAVFPPASHRSPISHEGSLSSAAMSPSFSPSLSPLAARSPVVSPFGVAQGPSASALSAESGLEPPDDTELHI

Target ORF information:

RefSeq Version	XM_002693149.4
Organism	Bos taurus(cattle)
Definition	Bos taurus dachsous cadherin-related 1 (DCHS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002693149.4

ORF Insert Sequence:

ATGCAGAAGG AGTGGGCCAC TGCACCTTCG TGCCCGGGCA TGCAGATCCC CAGGCCTCTC 
CCCCTGCTGT CATTGCTGAT GCTGCTGCTC CTGGGGGCGG GGGCTCCAGG TGCCTGGGGT 
CAGGCTGGGA GCTTGGACCT GCAGATTGAT GAGGAGCAGC CAGCGGGCAC GCTGATCGGG 
GACATCAGTG CAGGGCTTCC AGCGGGCACA GCAGCACCTC CCATGTACTT CATCTCTGCC 
CAGGAGGGCA GCGGGGTGGG CACGGACCTG GCCATCGATG AACACAGTGG GGTGGTCCGT 
ACAGCCCGTG TCTTGGACCG AGAGCGGCGG GATCGCTACC GCTTCACTGC AGTCACTCCC 
GACGGTGCCA CTGTGGAAGT TACCGTGCGA GTGGCTGACA TCAATGACCA TGCTCCGGCT 
TTCCCACAGG CTCGGGCTGT CCTGCAGATA CCTGAGCATA CAGCACTTGG CACCCGCTAC 
CCACTGGAGC CTGCTCGTGA TGCCGACGCT GGCCGCCTGG GAACGCAGGG CTATGCCCTG 
TCTGGTGATG GGGCTGGAGA GACCTTCCGG CTGGAGACAC GCCCTGGTCC AGATGGGGCC 
CCAGTGCCTG AGCTGGTAGT TACCGGAGAG CTGGACCGAG AGAACCGCTC ACACTACATG 
CTGCAACTGG AGGCCTATGA CGGTGGTTCA CCCCCTCGGA GGGCCCAGGC CCTGCTGGAC 
GTGACACTGC TAGACATCAA TGACCATGCC CCAGCTTTCA ATCAGAGCCG CTACCACGCT 
GCTGTGTCCG AGAGCCTGGC CCCCGGCAGT CCTGTCTTGC AGGTGTATGC CTCTGATGCC 
GATGCAGGTG CCAATGGGGC TGTAACTTAT GAGATCAACC GGAGGCAGAG CGAGGGTGAT 
GGACCCTTCT CCATTGATGC ACACACGGGG CTGCTGCGGC TGGAGCGGCC GCTGGACTTT 
GAGCAACGTC GGGTCCACGA ACTGGTGGTG CAGGCTCGGG ATGGAGGGGC TCACCCTGAG 
CTGGGCTCAG CCTTTGTGAC CGTGCATGTG CGAGATGCCA ATGACAATCA GCCCTCCATG 
ACTGTCATCT TCCTGAGTGC AGATGGCTCC CCCCGTGTAT CTGAGGCTGC CCCCCCGGGC 
CAGCTTGTTG CTCGAATCTC TGTGTCAGAC CCAGATGACG GTGACTTTGC GCATGTCAAC 
GTGTCCCTGG AGGGTGGAGA GGGCCACTTT GCGCTCAGCA CCCAGGACAG CGTCATCTAC 
CTGGTGTGTG TGGCTCGGAG GCTGGATCGG GAGGAGCGCG ACGCCTACAA CTTGCGAGTT 
ACTGCCACAG ACTCAGGCTC ACCCCCACTG CGGGCCGAGG CTGCCTTTGT GCTACACGTC 
ACTGATGTCA ATGACAATGC GCCTGCCTTT GACCGCCAGC TCTACCGACC CGAGCCTCTG 
CCTGAGGTTG CCCTGCCTGG CAGCTTCGTG GCGCGGGTGA CGGCCCGGGA TCCTGACCAG 
GGCACCAATG GTCAGGTCAC CTACAGCCTG GCCCCAGGCG CCCACACCCG CTGGTTCTCC 
ATAGACCCCA CCTCAGGCAT TGTCACTACA GCTGCTTTGC TGGACTATGA GTTGGAACCT 
CAGCCACAGC TAATCGTGGT GGCCACGGAT GGGGGCTTGC CCCCTCTCTC CTCCTCTGCC 
ACAGTGAGCG TGGTCTTGCA AGATGTGAAT GATAACGAGC CGCAGTTCCA GAGGACTTTC 
TACAACGCCT CGCTGCCTGA GGGCACTCAG CCAGGAACCT GCTTCCTGCA GGTGACAGCC 
ACAGATGCAG ACAGTGGCCC ATTTGGCCTC CTCTCCTATT CCTTGGGTGC CGGACTTGGG 
ACCTCAGGGT CTCCCCCGTT CCGCATCGAT GCCCACAGTG GTGACGTGTG TACGACCCGG 
ACCCTGGACC GCGATCAGGG GCCCTCAAGC TTTGACTTCA CAGTGACAGC TGTGGATGGG 
GGAGGCCTCA AGTCTATGGT ATATGTGAAG GTGTTTGTGT CAGACGAGAA TGACAACCCA 
CCTCAGTTTT ATCCACGGGA GTATGCTGCC AGTCTGAGCG CCCAGAGTGC ACCAGGCACA 
GCTGTGCTGA GGGTACGTGC CCATGACCCT GATCAGGGAC CCCATGGGCG ACTCTCCTAC 
CACATCCTGG CTGGCAACAG CCCCCCGCTC TTTGCCTTGG ACGAGCACTC AGGACTATTG 
ACAGTAGCCT GGCCCTTGGC CAGACGGGCC AGTTCCATGG TGCAGCTGGA GATCGGGGCT 
CGGGATGGAG GTGGCCTGCA GGCGGAGCCC AATGCCCGAG TCAACATCAG CATTGTGCCT 
GGAACTCCTG TACCACCAGT ATTTGAGCAG CTTCAGTATG TCTTTTCTGT GCCAGAGGAT 
GTGGCACCAG GCACCAGTGT GGGCGTAGTC CAGGCACACA ATCCACCAGG TCGCTTGGGA 
CCGGTGACCC TTGCCCTATC GGGGGGCGAT CCCCGAGGAC TCTTCTCCCT AGATGGGGCC 
ACTGGGCTGT TACAGACACT TCGCCCTCTG GACCGGGAGC TGCTGGGACC AGTGCTGGAG 
CTGGAGGTGC GGGCAGGCAG CGGAGTGCCA CCGGCTTTCG CTGTAGCTCG GGTGCGCGTG 
CTGCTGGACG ATGTGAATGA CAACTCCCCT GCCTTCCCTG CGCCTGAAGA CACAGTGCTG 
CTGCCATCGA GCACTGCCCC AGGGACCCTC ATCTATACAC TGCGGGCTCT GGACCCCGAC 
TCAGGAGTTA ACAGTCGAGT CACCTTTACC CTGCTTGCTG GGGGTGGTGG GACCTTCACT 
GTGGACCCCA CCACAGGCCA CATACGGCTT ATGGGACCTC TGGGGCCTCC GGGGGGGCCA 
GCCCATGAAC TGGAGCTGGA AGCCCGGGAT GGAGGCTCCC CACCTCGCAC CAGCCACTTT 
CGACTGCGGG TGGTGGTACA GGACTTGGGG ACCCGTGGGC TGGCTCCCCA CTTCGACAGC 
CCTACCTACC GTGTAGACCT GCCCTCAGGC ACCACCCCTG GAACTCAAGT CCTACAAGTG 
CAGGCTCGAG CACCAGATGG GAGCCCTGTC ACCTACCACC TTGCAGCAGA TGGGCCAGGT 
AGCCCCTTTG GCCTGGAACC ACAGAGTGGA TGGCTGTGGG TGCGGGCAGC ACTGGACCAT 
GAGGCCCAGG AGTTGTACAC ACTGAAAGTA ATGGCAGTGT CTGGATCCAA AGCTGAGTTG 
GGGCAGCAGA CAGCCACGGC CACCGTGAGG ATCAGCATCC TCAACCAGAA CGACCACAGT 
CCCCGCTTGT CTGAGGAGCC CACCTTCCTG GCTGTGGCTG AGAACCAGCC CCCAGGGACA 
AGCGTGGGTC GGGTCTTTGC CACTGACCGA GATTCAGGAA TCAACGGACG TCTGACCTAC 
AGCCTGCGAC AGCTGTCAGA AGACAGCAAG GCCTTCCGCA TCCACCCCCA GACTGGAGAG 
GTGACCACAC TACAAACTCT GGACCGTGAG CGGCAGAACA GCTACCAGCT TCTGGTGCAG 
GTTCAGGATG CAGGGAGCCC ACCCCGCAGC ACCACAGGCA CCGTGCACAT CGCAGTGCTT 
GACCTCAATG ACAACAGCCC TACCTTCCTG CAGGCCTCAG GGGCTGCTGG TGGAGGCCTC 
CCCATACAGG TACCAGATGG TGTGCCTCCA GGAACATTGG TGACAACCCT GCAGGCCAAG 
GATCCAGATG AGGGGGAGAA TGGAACCATC CTGTACATGC TAACTGGTCC TGGCTCAGAG 
CTCTTCTCCC TGCACCCTCA CTCGGGAGAG CTGCTCACGG CAGCACCCCT GATCCGAGCA 
GAGCGGCCAC ACTACGTGCT CACATTGAGC GCTCATGACC AAGGCAGCCC CCCTCGGAGC 
TCCAGCCTCC AGCTGCTAGT GCAGGTGCTT CCCTCCACTC GCTCGGCTGA GCCTGCACCG 
GAACCCTCAG AGCCAGACCC GGTGGCGCCT GTGCCTGTCG TGCTGACCGT GACAGCAGCC 
GAGGGGCTCC GGCCCGGCTC CCTGTTGGGC TCTGTGGCAC CGCTGGAGCA GGCGGGGGTG 
GGCGCCCTTA CCTACACACT GGTGGGCGGC GCCGACCCAG AGGGCACCTT TGCGCTGGAC 
GCAGCCTCTG GGCGCTTGTA CCTGGCGCGG CCCCTGGACT TCGAGGCGGG TCCGGCTTGG 
CGCGCGCTCA CGGTGCGCGC CGAGGGGCCG GGAGGCGCGG GCGCGCGGCT GCTGAGAGTG 
CAGGTGCGCG TGCAGGACGA GAACGAGCAC GCGCCGGCCT TCGCGCGCGA CCCGCTGGCG 
TTGGCGCTCC CAGAGAACCC GGAGCCGGGC GCGACCTTGT ACACTTTCCG CGCGTCCGAC 
GCCGACGGCC CCGGCCCCAA CAGCGATGTG CGCTACCGCC TGCTGCGCCA AGAGCCGCCT 
GTGCCCGCGC TTCGCCTGGA CGCGCGCACC GGAGCGCTCA GCGCCCCGCG AGGCCTGGAT 
CGGGAGACCA CTCCGGCTCT GCTGCTGCTC GTGGAAGCCA CTGACCGGCC CGCCAACGCC 
AGCCGCCGCC GCACAGCACG CGTTTCCGCG CGCGTCTTTG TCACGGATGA GAATGACAAT 
GCTCCAGTCT TTGCCTCGCC CTCACGTGTG CGCCTCCCTG AGGATCACCC GCCCGGGCCC 
ACGGCGCTGC ACGTGGTAGC CCGGGACCCC GACCTGGGCG AGGCCGCACG GGTGTCCTAT 
CGCCTGGCAG CTGGCGCGGA CGGCTGTTTC CGGCTACACC CCAGCACCGG AGCGCTGTCC 
GTGGTGCGGT CTCTGGACCG CGAACAGCGA GCTGAGTACG TGCTGACTGT GGTGGCCTCT 
GACCACGGCT CCCCACCGCG CTCGGCCACG CAGCTTCTGA CTGTCAGTGT CGCCGACGTC 
AACGACGAGG CACCCGCTTT CCAACAGCAG GAGTACAGCG TCCTCTTGCG GGAGAACAGC 
CCGCCTGGAA CGTCTCTGCT CACTCTGCAG GCAACCGACC CCGATCTGGG GGCCAATGGG 
CAAGTGACTT ATGGAGGCAT CTCTGGCGAA AACTTCTCCC TGGATCCAGA CACCGGGGTC 
CTTACCACTC TTCGGGCTCT GGATCGGGAG GAGCAGGAGG AGATCAACTT GACAGTATAT 
GCCCGGGACG GGGGCTCGCC TCCACTGTTG ACCCACGTCA CAGTGCGAGT GGCTGTGGAG 
GATGAGAATG ACCATGCCCC AACCTTTGGG AGTGCCCATC TCTCCCTGGA GGTGCCCGAG 
GGCCAGGACC CCCAGACCCT TACCACGCTG CGGGCCTCTG ACCCGGATGT GGGAGCCAAC 
GGGCAGCTGC AGTACCGCAT CCTGGATGGG GACCCCTCAG GAGCCTTTGT CCTGGACCTA 
GCTTCTGGGG AGTTTGGCAC CATGAGGCCA CTAGACCGGG AGGTGGAGCC AGCATTCCAG 
CTGCAGATAG AAGCCCGGGA TGGAGGCCAG CCAGCTCTCA GCGCTACGCT TCTTGTGACA 
GTGACAGTGC TAGATGCCAA TGACCATGCC CCAGCCTTCC CTGTGCCCTC CTACTCTGTG 
GAGGTGCCTG AGGACGCACC TGCAGGGACC CTGCTGCTGC AGCTGCAGGC TCATGATCCT 
GATGCTGGGG CCAATGGTCG TGTGACCTAC TACCTGGGTA CAGGTGCAGC AGGAGCCTTC 
CTGCTAGAGC CCAGCTCTGG GGAATTGCGC ACAGCCACCA CCCTTGACAG AGAGCAGTGT 
CCCAGCTATG CCTTTACAGT GAATGCAGTG GACAGTGCAG CTGCTGGGCC CCTAAGCACC 
ACAGTGCCCG TCACCATCAC AGTGCGTGAT GTCAATGACC ACGCGCCCAC CTTCCCCACC 
AGTCCCCTGA GGCTGCGCCT GCCCCGCCCA GGCCCTGTCT TCAGCACTCC AGTTCTGCCT 
CTGGCCACTC TGCGAGCTGA AGACCGTGAC GCTGGTGCCA ATGCCTCCAT CCTGTACCGG 
CTGGCAGGCA CACCACCTCC TGGCACCACC GTGGACTCTT ACACTGGTGA AATCCGTGTG 
TCCCGCTCCC CTGTATCTCT GAGCCCCCGA GATCGTGTCC TCTTCATTGT GGCAACTGAC 
CTTGGCCGTC CAGCTCGCTC TGCCACTGGT GTGGTCATTA TTGGGCTGCA AGGGGAGTCT 
GAGCATGGAC CCCGCTTCTC CCGAGCCAGT AGTGAAGCCA TGCTCCGTGA GAATGCACCC 
CCAGGGACTC CCGTTGTCTC CCCCAAGGCT ATCCATGCAG GGGGCTCAAG TGGACCCATC 
ACATACAGCA TTCTCAGTGG GAATGAGAAA GGGACATTCT CCATCCAGCC TAGTACAGGA 
GCCATCACAG TCCGCTCAGC AGAGGGGCTG GATTTTGAGG CAAACCCACG GCTGCGACTG 
GTGCTGCAGG CAGAGAGTGG AGGAGCCTAT GCCTTCTCTG TGCTGACCCT AACCCTGCAA 
GATGCCAATG ACAACGCGCC CCGCTTCCTG CAGCCCCACT ACGTGGCCTT CCTTCCAGAG 
TCCAGGCCCT TGGAGGGACC CCTGCTACAG GTGGAAGCAG ATGACCTGGA CCAAGGCCCC 
AGTGGACAGA TCTCCTACAG TCTGGCCGCA TCCCAGCCAG CCCGGGGATT GTTCCATGTA 
GACCCAGCCA CGGGCACTAT CACCACCACA GCTATCCTGG ACCGCGAGAT CTGGGCCGAA 
ACACGGCTGG TGCTGATGGC CACAGACAGA GGAAGCCCAG CCCTGGTGGG CTCAGCTACC 
CTGACGGTGA TGGTCATCGA CACCAATGAC AATCGCCCCA CCATCCCCCA GCCCTGGGAG 
CTCCGAGTGT CAGAAGATGC ACTGCTGGGC TCGGAGATCG CACAGGTAAC GGGGAATGAT 
GTGGACTCAG GACCAGTGCT GTGGTACGTT CTGAGCCCTT CTGGGCCCCA GGATCCCTTC 
AGTGTCGGCC GCTATGGAGG CCGCCTCTCC CTCACGGGCC CCTTGGATTT TGAGCAGCGC 
GACCGCTACC ACCTGCAGCT CCTGGCACAC GACGGGCCTC ACGAGGGCCG GGCCAACCTC 
ACGGTGTTCG TGGAGGATGT CAATGACAAC TCCCCTGCCT TCTCACAGAC TCTCTACCAG 
GTGATGCTGC TCGAGCACAC ACCCCCGGGC AGTGCCATTC TCTCCGTCTC GGCCACTGAC 
CGGGACTCGG GCGCCAACGG TCACGTCTCC TACCACCTGG CTTCCTCTGC TGAGGGCTTC 
AGCATTGACC CCAACAACGG GACCCTATTC ACAACAGTGG GGACAGTGGC CTTGGGCCAT 
GAAGGGCCAG GCATGGTGGA CGTGGTGCTG GAAGCCCGAG ACCACGGAGT GCCGGTTCGG 
GCCGCCCGAG CCACAGTGCA CGTGCAGCTG CAGGATCAGA ACGACCATGC CCCGAGCTTC 
ACGCTGCCAC ACTACCGCGT GGCTGTGACT GAGGATCTGC CGCCCGGCTC CACCCTGCTC 
ACCCTGGAGG CCACAGATGC CGACGGGAGC CGCAGCCATG CCGCCGTGGA CTACAGCATT 
GTCAGTGGCA ACCGGGGCCG AGTCTTTCAG CTGGAACCCC GGCTGGCGGA GGCTGGGGAG 
AGTGATGGAC TAGGCCCCCG GGCCCTGGGC TGCCTGGTGT TGCTTGAGCC TCTAGACTTT 
GAAAGCCTAA CCCAGTACAA CCTAACTGTG GCAGCAACTG ACCGGGGGCA GCCACCTCGA 
AGTTCGGCTG TGCCAGTCAC CGTCAGCGTG CTGGATGTCA ACGACAACCC ACCTGTCTTC 
ACCCGAGCAG CCTACCGTGT GGCGGTGCCT GAGGACACGC CTGTGGGAGC CGAGCTGCTG 
CACGTGGAGG CCTCCGACGC TGATCCAGGC CCCCATGGCC TTGTACGCTT CACCCTCAGC 
TCAGGCGACC CTCTGGGGCT CTTTGAGCTG GATGAGAGCT CCGGGGACTT GCGACTGGCC 
CGCCCGTTGG ACTGTGAGAC CCAAGCTCGA CATCAGCTTG TAGTGCAGGC TGCCGACCCT 
GCCGGGGCAC ACTTTGCTCT GGCACCAGTG ACCATTGAGG TCCAGGACGT GAATGACCAT 
GGCCCCGCCT TCCCTCTGAG CTTGCTCAGC ACCAGCCTGG CTGAGAACCA GCCTCCAGGC 
ACTCTTGTTA CCACCCTGCA TGCAATTGAT GGGGATGCTG GGGCTTTCGG AAGGCTCCAC 
TACAGCCTGC TGGAGGCTGG GCCGGGGCCC GAGGGCCGCG AGGCGTTTGC GCTCAACAGC 
TCAACTGGGG AGTTGCGGGC ACGAGTGGCC TTTGACTACG AGCACACTGG CAGCTTCCAG 
CTGCTGGTGG GCGCTACGGA TGCCGGGAAC CTGTCCGCCT CAGTCACTGT GTCAGTGCTG 
GTGACCGGTG AGGATGAGTA TGACCCAGTG TTCCTGGCTC CAGCTTTCCA CTTTCAGGTG 
CCAGAAGGTG CACGGCGTGG CCACAGCCTG GGTCACGTGC AGGCCACAGA CGAGGATGGA 
GGTGCTGACG GCCTGGTGCT GTACTCGCTG GCCGCCTCGT CCCCCTATTT TGGTATCAAC 
CAGACTACAG GTGCCCTGTA CCTGCGGGTG GACAGCCGGG CACCAGGCAG TGGAACAGGC 
ACCTCCGGGG GTGGGGGCCG GACCCGACGT GAGGCGCCAC GGGAGCTGAG GCTGGAGGTG 
GTGGCAAGGG GGCCTCTGCC CGGTTCTCGG AGTGCCACCG TGCCTGTGAC TGTGGACATC 
ACCCACACTG CGCTGGGCCT GGCACCTGAC CTCAACCTGC TCTTGGTGGG AGCCGTGGCA 
GCCTCTCTGG GAGTCGTGGT GGTGCTTGCC CTGGCAGCCC TTGTCCTGGG ACTGGTGCGG 
GCCCGGAGCC GCAAAGCTGA GGCGGCGCCT GGCCCGATGT CCCAGGCGGC ACCCCTGGCC 
AGTGGCTCAC TGCAGAAGCT GGGCCGAGAG CCACCCAGCC CGCCGCCCTC AGAGCACCTG 
TATCACCAGA CCCTCCCCAG CTATGGTGGG CCTGGAGCTG GAGGACCCTA CCCCCGCGGT 
GGCTCCCTGG ACCCTTCACA CTCAAGCGGC CGAGGCTCAG CAGAGGCTGC GGAAGATGAC 
GAGATCCGCA TGATCAACGA GTTCCCCCGC GTGGCCAGTG TGGCCTCCTC CCTGGCTGCC 
CGCGGCCCTG ACTCCGGCAT CCAGCAGGAT GCAGACGGAC TGAGTGACAC CTCCTGTGAG 
CCACCTGCCC CTGACACCTG GTATAAGGGC CGTAAGGCAG GGCTGCTGCT GCCGGGTGCA 
GGAGCCACTC TGTACAGAGA AGACGGCCCC CCAGCCACTG CCACCACCTT CCTGGGGGGC 
TGTGGCCTAA GCCCCGCGCC CACCGGGGAC TACGGCTTTC CAGCAGATGG CAAGCCCTGT 
GTGGCGGGAG CGCTGACGGC CATCGTGGCC GGTGAGGAGG AGCTCCGTGG CAGCTACAAC 
TGGGACTACC TGCTGAGCTG GTGCCCTCAG TTCCAGCCGC TGGCCAGTGT CTTCACAGAG 
ATTGCCCGGC TCAAGGATGA AGCTCGGCCG TGTCCCCCGG CGCCCCGTAT TGACCCACCG 
CCCCTCATCA CTGCCGTGGC CCACCCAGGA GCCAAGTCTG TGCCCCCCAA ACCAGCCAGC 
ACGGCCGCAA CCCGGGCCGT CTTCCCACCA GCCTCTCACC GCTCCCCCAT CAGCCACGAA 
GGTTCCCTGT CCTCGGCGGC CATGTCTCCC AGCTTCTCAC CTTCACTATC TCCTCTGGCC 
GCTCGCTCAC CCGTCGTCTC ACCATTTGGG GTGGCCCAGG GCCCCTCAGC CTCAGCTCTG 
AGCGCAGAGT CTGGCCTGGA GCCACCAGAC GACACCGAGC TCCATATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCHS1 ( XM_002701345.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002701391.2 Bos taurus dachsous cadherin-related 1 (DCHS1), mRNA.

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CloneID	OBa26442
Clone ID Related Accession (Same CDS sequence)	XM_002693149.4 , XM_002701345.4
Accession Version	XM_002701345.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protocadherin-16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646260.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002701345.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG AGTGGGCCAC TGCACCTTCG TGCCCGGGCA TGCAGATCCC CAGGCCTCTC 
CCCCTGCTGT CATTGCTGAT GCTGCTGCTC CTGGGGGCGG GGGCTCCAGG TGCCTGGGGT 
CAGGCTGGGA GCTTGGACCT GCAGATTGAT GAGGAGCAGC CAGCGGGCAC GCTGATCGGG 
GACATCAGTG CAGGGCTTCC AGCGGGCACA GCAGCACCTC CCATGTACTT CATCTCTGCC 
CAGGAGGGCA GCGGGGTGGG CACGGACCTG GCCATCGATG AACACAGTGG GGTGGTCCGT 
ACAGCCCGTG TCTTGGACCG AGAGCGGCGG GATCGCTACC GCTTCACTGC AGTCACTCCC 
GACGGTGCCA CTGTGGAAGT TACCGTGCGA GTGGCTGACA TCAATGACCA TGCTCCGGCT 
TTCCCACAGG CTCGGGCTGT CCTGCAGATA CCTGAGCATA CAGCACTTGG CACCCGCTAC 
CCACTGGAGC CTGCTCGTGA TGCCGACGCT GGCCGCCTGG GAACGCAGGG CTATGCCCTG 
TCTGGTGATG GGGCTGGAGA GACCTTCCGG CTGGAGACAC GCCCTGGTCC AGATGGGGCC 
CCAGTGCCTG AGCTGGTAGT TACCGGAGAG CTGGACCGAG AGAACCGCTC ACACTACATG 
CTGCAACTGG AGGCCTATGA CGGTGGTTCA CCCCCTCGGA GGGCCCAGGC CCTGCTGGAC 
GTGACACTGC TAGACATCAA TGACCATGCC CCAGCTTTCA ATCAGAGCCG CTACCACGCT 
GCTGTGTCCG AGAGCCTGGC CCCCGGCAGT CCTGTCTTGC AGGTGTATGC CTCTGATGCC 
GATGCAGGTG CCAATGGGGC TGTAACTTAT GAGATCAACC GGAGGCAGAG CGAGGGTGAT 
GGACCCTTCT CCATTGATGC ACACACGGGG CTGCTGCGGC TGGAGCGGCC GCTGGACTTT 
GAGCAACGTC GGGTCCACGA ACTGGTGGTG CAGGCTCGGG ATGGAGGGGC TCACCCTGAG 
CTGGGCTCAG CCTTTGTGAC CGTGCATGTG CGAGATGCCA ATGACAATCA GCCCTCCATG 
ACTGTCATCT TCCTGAGTGC AGATGGCTCC CCCCGTGTAT CTGAGGCTGC CCCCCCGGGC 
CAGCTTGTTG CTCGAATCTC TGTGTCAGAC CCAGATGACG GTGACTTTGC GCATGTCAAC 
GTGTCCCTGG AGGGTGGAGA GGGCCACTTT GCGCTCAGCA CCCAGGACAG CGTCATCTAC 
CTGGTGTGTG TGGCTCGGAG GCTGGATCGG GAGGAGCGCG ACGCCTACAA CTTGCGAGTT 
ACTGCCACAG ACTCAGGCTC ACCCCCACTG CGGGCCGAGG CTGCCTTTGT GCTACACGTC 
ACTGATGTCA ATGACAATGC GCCTGCCTTT GACCGCCAGC TCTACCGACC CGAGCCTCTG 
CCTGAGGTTG CCCTGCCTGG CAGCTTCGTG GCGCGGGTGA CGGCCCGGGA TCCTGACCAG 
GGCACCAATG GTCAGGTCAC CTACAGCCTG GCCCCAGGCG CCCACACCCG CTGGTTCTCC 
ATAGACCCCA CCTCAGGCAT TGTCACTACA GCTGCTTTGC TGGACTATGA GTTGGAACCT 
CAGCCACAGC TAATCGTGGT GGCCACGGAT GGGGGCTTGC CCCCTCTCTC CTCCTCTGCC 
ACAGTGAGCG TGGTCTTGCA AGATGTGAAT GATAACGAGC CGCAGTTCCA GAGGACTTTC 
TACAACGCCT CGCTGCCTGA GGGCACTCAG CCAGGAACCT GCTTCCTGCA GGTGACAGCC 
ACAGATGCAG ACAGTGGCCC ATTTGGCCTC CTCTCCTATT CCTTGGGTGC CGGACTTGGG 
ACCTCAGGGT CTCCCCCGTT CCGCATCGAT GCCCACAGTG GTGACGTGTG TACGACCCGG 
ACCCTGGACC GCGATCAGGG GCCCTCAAGC TTTGACTTCA CAGTGACAGC TGTGGATGGG 
GGAGGCCTCA AGTCTATGGT ATATGTGAAG GTGTTTGTGT CAGACGAGAA TGACAACCCA 
CCTCAGTTTT ATCCACGGGA GTATGCTGCC AGTCTGAGCG CCCAGAGTGC ACCAGGCACA 
GCTGTGCTGA GGGTACGTGC CCATGACCCT GATCAGGGAC CCCATGGGCG ACTCTCCTAC 
CACATCCTGG CTGGCAACAG CCCCCCGCTC TTTGCCTTGG ACGAGCACTC AGGACTATTG 
ACAGTAGCCT GGCCCTTGGC CAGACGGGCC AGTTCCATGG TGCAGCTGGA GATCGGGGCT 
CGGGATGGAG GTGGCCTGCA GGCGGAGCCC AATGCCCGAG TCAACATCAG CATTGTGCCT 
GGAACTCCTG TACCACCAGT ATTTGAGCAG CTTCAGTATG TCTTTTCTGT GCCAGAGGAT 
GTGGCACCAG GCACCAGTGT GGGCGTAGTC CAGGCACACA ATCCACCAGG TCGCTTGGGA 
CCGGTGACCC TTGCCCTATC GGGGGGCGAT CCCCGAGGAC TCTTCTCCCT AGATGGGGCC 
ACTGGGCTGT TACAGACACT TCGCCCTCTG GACCGGGAGC TGCTGGGACC AGTGCTGGAG 
CTGGAGGTGC GGGCAGGCAG CGGAGTGCCA CCGGCTTTCG CTGTAGCTCG GGTGCGCGTG 
CTGCTGGACG ATGTGAATGA CAACTCCCCT GCCTTCCCTG CGCCTGAAGA CACAGTGCTG 
CTGCCATCGA GCACTGCCCC AGGGACCCTC ATCTATACAC TGCGGGCTCT GGACCCCGAC 
TCAGGAGTTA ACAGTCGAGT CACCTTTACC CTGCTTGCTG GGGGTGGTGG GACCTTCACT 
GTGGACCCCA CCACAGGCCA CATACGGCTT ATGGGACCTC TGGGGCCTCC GGGGGGGCCA 
GCCCATGAAC TGGAGCTGGA AGCCCGGGAT GGAGGCTCCC CACCTCGCAC CAGCCACTTT 
CGACTGCGGG TGGTGGTACA GGACTTGGGG ACCCGTGGGC TGGCTCCCCA CTTCGACAGC 
CCTACCTACC GTGTAGACCT GCCCTCAGGC ACCACCCCTG GAACTCAAGT CCTACAAGTG 
CAGGCTCGAG CACCAGATGG GAGCCCTGTC ACCTACCACC TTGCAGCAGA TGGGCCAGGT 
AGCCCCTTTG GCCTGGAACC ACAGAGTGGA TGGCTGTGGG TGCGGGCAGC ACTGGACCAT 
GAGGCCCAGG AGTTGTACAC ACTGAAAGTA ATGGCAGTGT CTGGATCCAA AGCTGAGTTG 
GGGCAGCAGA CAGCCACGGC CACCGTGAGG ATCAGCATCC TCAACCAGAA CGACCACAGT 
CCCCGCTTGT CTGAGGAGCC CACCTTCCTG GCTGTGGCTG AGAACCAGCC CCCAGGGACA 
AGCGTGGGTC GGGTCTTTGC CACTGACCGA GATTCAGGAA TCAACGGACG TCTGACCTAC 
AGCCTGCGAC AGCTGTCAGA AGACAGCAAG GCCTTCCGCA TCCACCCCCA GACTGGAGAG 
GTGACCACAC TACAAACTCT GGACCGTGAG CGGCAGAACA GCTACCAGCT TCTGGTGCAG 
GTTCAGGATG CAGGGAGCCC ACCCCGCAGC ACCACAGGCA CCGTGCACAT CGCAGTGCTT 
GACCTCAATG ACAACAGCCC TACCTTCCTG CAGGCCTCAG GGGCTGCTGG TGGAGGCCTC 
CCCATACAGG TACCAGATGG TGTGCCTCCA GGAACATTGG TGACAACCCT GCAGGCCAAG 
GATCCAGATG AGGGGGAGAA TGGAACCATC CTGTACATGC TAACTGGTCC TGGCTCAGAG 
CTCTTCTCCC TGCACCCTCA CTCGGGAGAG CTGCTCACGG CAGCACCCCT GATCCGAGCA 
GAGCGGCCAC ACTACGTGCT CACATTGAGC GCTCATGACC AAGGCAGCCC CCCTCGGAGC 
TCCAGCCTCC AGCTGCTAGT GCAGGTGCTT CCCTCCACTC GCTCGGCTGA GCCTGCACCG 
GAACCCTCAG AGCCAGACCC GGTGGCGCCT GTGCCTGTCG TGCTGACCGT GACAGCAGCC 
GAGGGGCTCC GGCCCGGCTC CCTGTTGGGC TCTGTGGCAC CGCTGGAGCA GGCGGGGGTG 
GGCGCCCTTA CCTACACACT GGTGGGCGGC GCCGACCCAG AGGGCACCTT TGCGCTGGAC 
GCAGCCTCTG GGCGCTTGTA CCTGGCGCGG CCCCTGGACT TCGAGGCGGG TCCGGCTTGG 
CGCGCGCTCA CGGTGCGCGC CGAGGGGCCG GGAGGCGCGG GCGCGCGGCT GCTGAGAGTG 
CAGGTGCGCG TGCAGGACGA GAACGAGCAC GCGCCGGCCT TCGCGCGCGA CCCGCTGGCG 
TTGGCGCTCC CAGAGAACCC GGAGCCGGGC GCGACCTTGT ACACTTTCCG CGCGTCCGAC 
GCCGACGGCC CCGGCCCCAA CAGCGATGTG CGCTACCGCC TGCTGCGCCA AGAGCCGCCT 
GTGCCCGCGC TTCGCCTGGA CGCGCGCACC GGAGCGCTCA GCGCCCCGCG AGGCCTGGAT 
CGGGAGACCA CTCCGGCTCT GCTGCTGCTC GTGGAAGCCA CTGACCGGCC CGCCAACGCC 
AGCCGCCGCC GCACAGCACG CGTTTCCGCG CGCGTCTTTG TCACGGATGA GAATGACAAT 
GCTCCAGTCT TTGCCTCGCC CTCACGTGTG CGCCTCCCTG AGGATCACCC GCCCGGGCCC 
ACGGCGCTGC ACGTGGTAGC CCGGGACCCC GACCTGGGCG AGGCCGCACG GGTGTCCTAT 
CGCCTGGCAG CTGGCGCGGA CGGCTGTTTC CGGCTACACC CCAGCACCGG AGCGCTGTCC 
GTGGTGCGGT CTCTGGACCG CGAACAGCGA GCTGAGTACG TGCTGACTGT GGTGGCCTCT 
GACCACGGCT CCCCACCGCG CTCGGCCACG CAGCTTCTGA CTGTCAGTGT CGCCGACGTC 
AACGACGAGG CACCCGCTTT CCAACAGCAG GAGTACAGCG TCCTCTTGCG GGAGAACAGC 
CCGCCTGGAA CGTCTCTGCT CACTCTGCAG GCAACCGACC CCGATCTGGG GGCCAATGGG 
CAAGTGACTT ATGGAGGCAT CTCTGGCGAA AACTTCTCCC TGGATCCAGA CACCGGGGTC 
CTTACCACTC TTCGGGCTCT GGATCGGGAG GAGCAGGAGG AGATCAACTT GACAGTATAT 
GCCCGGGACG GGGGCTCGCC TCCACTGTTG ACCCACGTCA CAGTGCGAGT GGCTGTGGAG 
GATGAGAATG ACCATGCCCC AACCTTTGGG AGTGCCCATC TCTCCCTGGA GGTGCCCGAG 
GGCCAGGACC CCCAGACCCT TACCACGCTG CGGGCCTCTG ACCCGGATGT GGGAGCCAAC 
GGGCAGCTGC AGTACCGCAT CCTGGATGGG GACCCCTCAG GAGCCTTTGT CCTGGACCTA 
GCTTCTGGGG AGTTTGGCAC CATGAGGCCA CTAGACCGGG AGGTGGAGCC AGCATTCCAG 
CTGCAGATAG AAGCCCGGGA TGGAGGCCAG CCAGCTCTCA GCGCTACGCT TCTTGTGACA 
GTGACAGTGC TAGATGCCAA TGACCATGCC CCAGCCTTCC CTGTGCCCTC CTACTCTGTG 
GAGGTGCCTG AGGACGCACC TGCAGGGACC CTGCTGCTGC AGCTGCAGGC TCATGATCCT 
GATGCTGGGG CCAATGGTCG TGTGACCTAC TACCTGGGTA CAGGTGCAGC AGGAGCCTTC 
CTGCTAGAGC CCAGCTCTGG GGAATTGCGC ACAGCCACCA CCCTTGACAG AGAGCAGTGT 
CCCAGCTATG CCTTTACAGT GAATGCAGTG GACAGTGCAG CTGCTGGGCC CCTAAGCACC 
ACAGTGCCCG TCACCATCAC AGTGCGTGAT GTCAATGACC ACGCGCCCAC CTTCCCCACC 
AGTCCCCTGA GGCTGCGCCT GCCCCGCCCA GGCCCTGTCT TCAGCACTCC AGTTCTGCCT 
CTGGCCACTC TGCGAGCTGA AGACCGTGAC GCTGGTGCCA ATGCCTCCAT CCTGTACCGG 
CTGGCAGGCA CACCACCTCC TGGCACCACC GTGGACTCTT ACACTGGTGA AATCCGTGTG 
TCCCGCTCCC CTGTATCTCT GAGCCCCCGA GATCGTGTCC TCTTCATTGT GGCAACTGAC 
CTTGGCCGTC CAGCTCGCTC TGCCACTGGT GTGGTCATTA TTGGGCTGCA AGGGGAGTCT 
GAGCATGGAC CCCGCTTCTC CCGAGCCAGT AGTGAAGCCA TGCTCCGTGA GAATGCACCC 
CCAGGGACTC CCGTTGTCTC CCCCAAGGCT ATCCATGCAG GGGGCTCAAG TGGACCCATC 
ACATACAGCA TTCTCAGTGG GAATGAGAAA GGGACATTCT CCATCCAGCC TAGTACAGGA 
GCCATCACAG TCCGCTCAGC AGAGGGGCTG GATTTTGAGG CAAACCCACG GCTGCGACTG 
GTGCTGCAGG CAGAGAGTGG AGGAGCCTAT GCCTTCTCTG TGCTGACCCT AACCCTGCAA 
GATGCCAATG ACAACGCGCC CCGCTTCCTG CAGCCCCACT ACGTGGCCTT CCTTCCAGAG 
TCCAGGCCCT TGGAGGGACC CCTGCTACAG GTGGAAGCAG ATGACCTGGA CCAAGGCCCC 
AGTGGACAGA TCTCCTACAG TCTGGCCGCA TCCCAGCCAG CCCGGGGATT GTTCCATGTA 
GACCCAGCCA CGGGCACTAT CACCACCACA GCTATCCTGG ACCGCGAGAT CTGGGCCGAA 
ACACGGCTGG TGCTGATGGC CACAGACAGA GGAAGCCCAG CCCTGGTGGG CTCAGCTACC 
CTGACGGTGA TGGTCATCGA CACCAATGAC AATCGCCCCA CCATCCCCCA GCCCTGGGAG 
CTCCGAGTGT CAGAAGATGC ACTGCTGGGC TCGGAGATCG CACAGGTAAC GGGGAATGAT 
GTGGACTCAG GACCAGTGCT GTGGTACGTT CTGAGCCCTT CTGGGCCCCA GGATCCCTTC 
AGTGTCGGCC GCTATGGAGG CCGCCTCTCC CTCACGGGCC CCTTGGATTT TGAGCAGCGC 
GACCGCTACC ACCTGCAGCT CCTGGCACAC GACGGGCCTC ACGAGGGCCG GGCCAACCTC 
ACGGTGTTCG TGGAGGATGT CAATGACAAC TCCCCTGCCT TCTCACAGAC TCTCTACCAG 
GTGATGCTGC TCGAGCACAC ACCCCCGGGC AGTGCCATTC TCTCCGTCTC GGCCACTGAC 
CGGGACTCGG GCGCCAACGG TCACGTCTCC TACCACCTGG CTTCCTCTGC TGAGGGCTTC 
AGCATTGACC CCAACAACGG GACCCTATTC ACAACAGTGG GGACAGTGGC CTTGGGCCAT 
GAAGGGCCAG GCATGGTGGA CGTGGTGCTG GAAGCCCGAG ACCACGGAGT GCCGGTTCGG 
GCCGCCCGAG CCACAGTGCA CGTGCAGCTG CAGGATCAGA ACGACCATGC CCCGAGCTTC 
ACGCTGCCAC ACTACCGCGT GGCTGTGACT GAGGATCTGC CGCCCGGCTC CACCCTGCTC 
ACCCTGGAGG CCACAGATGC CGACGGGAGC CGCAGCCATG CCGCCGTGGA CTACAGCATT 
GTCAGTGGCA ACCGGGGCCG AGTCTTTCAG CTGGAACCCC GGCTGGCGGA GGCTGGGGAG 
AGTGATGGAC TAGGCCCCCG GGCCCTGGGC TGCCTGGTGT TGCTTGAGCC TCTAGACTTT 
GAAAGCCTAA CCCAGTACAA CCTAACTGTG GCAGCAACTG ACCGGGGGCA GCCACCTCGA 
AGTTCGGCTG TGCCAGTCAC CGTCAGCGTG CTGGATGTCA ACGACAACCC ACCTGTCTTC 
ACCCGAGCAG CCTACCGTGT GGCGGTGCCT GAGGACACGC CTGTGGGAGC CGAGCTGCTG 
CACGTGGAGG CCTCCGACGC TGATCCAGGC CCCCATGGCC TTGTACGCTT CACCCTCAGC 
TCAGGCGACC CTCTGGGGCT CTTTGAGCTG GATGAGAGCT CCGGGGACTT GCGACTGGCC 
CGCCCGTTGG ACTGTGAGAC CCAAGCTCGA CATCAGCTTG TAGTGCAGGC TGCCGACCCT 
GCCGGGGCAC ACTTTGCTCT GGCACCAGTG ACCATTGAGG TCCAGGACGT GAATGACCAT 
GGCCCCGCCT TCCCTCTGAG CTTGCTCAGC ACCAGCCTGG CTGAGAACCA GCCTCCAGGC 
ACTCTTGTTA CCACCCTGCA TGCAATTGAT GGGGATGCTG GGGCTTTCGG AAGGCTCCAC 
TACAGCCTGC TGGAGGCTGG GCCGGGGCCC GAGGGCCGCG AGGCGTTTGC GCTCAACAGC 
TCAACTGGGG AGTTGCGGGC ACGAGTGGCC TTTGACTACG AGCACACTGG CAGCTTCCAG 
CTGCTGGTGG GCGCTACGGA TGCCGGGAAC CTGTCCGCCT CAGTCACTGT GTCAGTGCTG 
GTGACCGGTG AGGATGAGTA TGACCCAGTG TTCCTGGCTC CAGCTTTCCA CTTTCAGGTG 
CCAGAAGGTG CACGGCGTGG CCACAGCCTG GGTCACGTGC AGGCCACAGA CGAGGATGGA 
GGTGCTGACG GCCTGGTGCT GTACTCGCTG GCCGCCTCGT CCCCCTATTT TGGTATCAAC 
CAGACTACAG GTGCCCTGTA CCTGCGGGTG GACAGCCGGG CACCAGGCAG TGGAACAGGC 
ACCTCCGGGG GTGGGGGCCG GACCCGACGT GAGGCGCCAC GGGAGCTGAG GCTGGAGGTG 
GTGGCAAGGG GGCCTCTGCC CGGTTCTCGG AGTGCCACCG TGCCTGTGAC TGTGGACATC 
ACCCACACTG CGCTGGGCCT GGCACCTGAC CTCAACCTGC TCTTGGTGGG AGCCGTGGCA 
GCCTCTCTGG GAGTCGTGGT GGTGCTTGCC CTGGCAGCCC TTGTCCTGGG ACTGGTGCGG 
GCCCGGAGCC GCAAAGCTGA GGCGGCGCCT GGCCCGATGT CCCAGGCGGC ACCCCTGGCC 
AGTGGCTCAC TGCAGAAGCT GGGCCGAGAG CCACCCAGCC CGCCGCCCTC AGAGCACCTG 
TATCACCAGA CCCTCCCCAG CTATGGTGGG CCTGGAGCTG GAGGACCCTA CCCCCGCGGT 
GGCTCCCTGG ACCCTTCACA CTCAAGCGGC CGAGGCTCAG CAGAGGCTGC GGAAGATGAC 
GAGATCCGCA TGATCAACGA GTTCCCCCGC GTGGCCAGTG TGGCCTCCTC CCTGGCTGCC 
CGCGGCCCTG ACTCCGGCAT CCAGCAGGAT GCAGACGGAC TGAGTGACAC CTCCTGTGAG 
CCACCTGCCC CTGACACCTG GTATAAGGGC CGTAAGGCAG GGCTGCTGCT GCCGGGTGCA 
GGAGCCACTC TGTACAGAGA AGACGGCCCC CCAGCCACTG CCACCACCTT CCTGGGGGGC 
TGTGGCCTAA GCCCCGCGCC CACCGGGGAC TACGGCTTTC CAGCAGATGG CAAGCCCTGT 
GTGGCGGGAG CGCTGACGGC CATCGTGGCC GGTGAGGAGG AGCTCCGTGG CAGCTACAAC 
TGGGACTACC TGCTGAGCTG GTGCCCTCAG TTCCAGCCGC TGGCCAGTGT CTTCACAGAG 
ATTGCCCGGC TCAAGGATGA AGCTCGGCCG TGTCCCCCGG CGCCCCGTAT TGACCCACCG 
CCCCTCATCA CTGCCGTGGC CCACCCAGGA GCCAAGTCTG TGCCCCCCAA ACCAGCCAGC 
ACGGCCGCAA CCCGGGCCGT CTTCCCACCA GCCTCTCACC GCTCCCCCAT CAGCCACGAA 
GGTTCCCTGT CCTCGGCGGC CATGTCTCCC AGCTTCTCAC CTTCACTATC TCCTCTGGCC 
GCTCGCTCAC CCGTCGTCTC ACCATTTGGG GTGGCCCAGG GCCCCTCAGC CTCAGCTCTG 
AGCGCAGAGT CTGGCCTGGA GCCACCAGAC GACACCGAGC TCCATATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002701391.2
CDS	259..10149
Translation	MQKEWATAPSCPGMQIPRPLPLLSLLMLLLLGAGAPGAWGQAGSLDLQIDEEQPAGTLIGDISAGLPAGTAAPPMYFISAQEGSGVGTDLAIDEHSGVVRTARVLDRERRDRYRFTAVTPDGATVEVTVRVADINDHAPAFPQARAVLQIPEHTALGTRYPLEPARDADAGRLGTQGYALSGDGAGETFRLETRPGPDGAPVPELVVTGELDRENRSHYMLQLEAYDGGSPPRRAQALLDVTLLDINDHAPAFNQSRYHAAVSESLAPGSPVLQVYASDADAGANGAVTYEINRRQSEGDGPFSIDAHTGLLRLERPLDFEQRRVHELVVQARDGGAHPELGSAFVTVHVRDANDNQPSMTVIFLSADGSPRVSEAAPPGQLVARISVSDPDDGDFAHVNVSLEGGEGHFALSTQDSVIYLVCVARRLDREERDAYNLRVTATDSGSPPLRAEAAFVLHVTDVNDNAPAFDRQLYRPEPLPEVALPGSFVARVTARDPDQGTNGQVTYSLAPGAHTRWFSIDPTSGIVTTAALLDYELEPQPQLIVVATDGGLPPLSSSATVSVVLQDVNDNEPQFQRTFYNASLPEGTQPGTCFLQVTATDADSGPFGLLSYSLGAGLGTSGSPPFRIDAHSGDVCTTRTLDRDQGPSSFDFTVTAVDGGGLKSMVYVKVFVSDENDNPPQFYPREYAASLSAQSAPGTAVLRVRAHDPDQGPHGRLSYHILAGNSPPLFALDEHSGLLTVAWPLARRASSMVQLEIGARDGGGLQAEPNARVNISIVPGTPVPPVFEQLQYVFSVPEDVAPGTSVGVVQAHNPPGRLGPVTLALSGGDPRGLFSLDGATGLLQTLRPLDRELLGPVLELEVRAGSGVPPAFAVARVRVLLDDVNDNSPAFPAPEDTVLLPSSTAPGTLIYTLRALDPDSGVNSRVTFTLLAGGGGTFTVDPTTGHIRLMGPLGPPGGPAHELELEARDGGSPPRTSHFRLRVVVQDLGTRGLAPHFDSPTYRVDLPSGTTPGTQVLQVQARAPDGSPVTYHLAADGPGSPFGLEPQSGWLWVRAALDHEAQELYTLKVMAVSGSKAELGQQTATATVRISILNQNDHSPRLSEEPTFLAVAENQPPGTSVGRVFATDRDSGINGRLTYSLRQLSEDSKAFRIHPQTGEVTTLQTLDRERQNSYQLLVQVQDAGSPPRSTTGTVHIAVLDLNDNSPTFLQASGAAGGGLPIQVPDGVPPGTLVTTLQAKDPDEGENGTILYMLTGPGSELFSLHPHSGELLTAAPLIRAERPHYVLTLSAHDQGSPPRSSSLQLLVQVLPSTRSAEPAPEPSEPDPVAPVPVVLTVTAAEGLRPGSLLGSVAPLEQAGVGALTYTLVGGADPEGTFALDAASGRLYLARPLDFEAGPAWRALTVRAEGPGGAGARLLRVQVRVQDENEHAPAFARDPLALALPENPEPGATLYTFRASDADGPGPNSDVRYRLLRQEPPVPALRLDARTGALSAPRGLDRETTPALLLLVEATDRPANASRRRTARVSARVFVTDENDNAPVFASPSRVRLPEDHPPGPTALHVVARDPDLGEAARVSYRLAAGADGCFRLHPSTGALSVVRSLDREQRAEYVLTVVASDHGSPPRSATQLLTVSVADVNDEAPAFQQQEYSVLLRENSPPGTSLLTLQATDPDLGANGQVTYGGISGENFSLDPDTGVLTTLRALDREEQEEINLTVYARDGGSPPLLTHVTVRVAVEDENDHAPTFGSAHLSLEVPEGQDPQTLTTLRASDPDVGANGQLQYRILDGDPSGAFVLDLASGEFGTMRPLDREVEPAFQLQIEARDGGQPALSATLLVTVTVLDANDHAPAFPVPSYSVEVPEDAPAGTLLLQLQAHDPDAGANGRVTYYLGTGAAGAFLLEPSSGELRTATTLDREQCPSYAFTVNAVDSAAAGPLSTTVPVTITVRDVNDHAPTFPTSPLRLRLPRPGPVFSTPVLPLATLRAEDRDAGANASILYRLAGTPPPGTTVDSYTGEIRVSRSPVSLSPRDRVLFIVATDLGRPARSATGVVIIGLQGESEHGPRFSRASSEAMLRENAPPGTPVVSPKAIHAGGSSGPITYSILSGNEKGTFSIQPSTGAITVRSAEGLDFEANPRLRLVLQAESGGAYAFSVLTLTLQDANDNAPRFLQPHYVAFLPESRPLEGPLLQVEADDLDQGPSGQISYSLAASQPARGLFHVDPATGTITTTAILDREIWAETRLVLMATDRGSPALVGSATLTVMVIDTNDNRPTIPQPWELRVSEDALLGSEIAQVTGNDVDSGPVLWYVLSPSGPQDPFSVGRYGGRLSLTGPLDFEQRDRYHLQLLAHDGPHEGRANLTVFVEDVNDNSPAFSQTLYQVMLLEHTPPGSAILSVSATDRDSGANGHVSYHLASSAEGFSIDPNNGTLFTTVGTVALGHEGPGMVDVVLEARDHGVPVRAARATVHVQLQDQNDHAPSFTLPHYRVAVTEDLPPGSTLLTLEATDADGSRSHAAVDYSIVSGNRGRVFQLEPRLAEAGESDGLGPRALGCLVLLEPLDFESLTQYNLTVAATDRGQPPRSSAVPVTVSVLDVNDNPPVFTRAAYRVAVPEDTPVGAELLHVEASDADPGPHGLVRFTLSSGDPLGLFELDESSGDLRLARPLDCETQARHQLVVQAADPAGAHFALAPVTIEVQDVNDHGPAFPLSLLSTSLAENQPPGTLVTTLHAIDGDAGAFGRLHYSLLEAGPGPEGREAFALNSSTGELRARVAFDYEHTGSFQLLVGATDAGNLSASVTVSVLVTGEDEYDPVFLAPAFHFQVPEGARRGHSLGHVQATDEDGGADGLVLYSLAASSPYFGINQTTGALYLRVDSRAPGSGTGTSGGGGRTRREAPRELRLEVVARGPLPGSRSATVPVTVDITHTALGLAPDLNLLLVGAVAASLGVVVVLALAALVLGLVRARSRKAEAAPGPMSQAAPLASGSLQKLGREPPSPPPSEHLYHQTLPSYGGPGAGGPYPRGGSLDPSHSSGRGSAEAAEDDEIRMINEFPRVASVASSLAARGPDSGIQQDADGLSDTSCEPPAPDTWYKGRKAGLLLPGAGATLYREDGPPATATTFLGGCGLSPAPTGDYGFPADGKPCVAGALTAIVAGEEELRGSYNWDYLLSWCPQFQPLASVFTEIARLKDEARPCPPAPRIDPPPLITAVAHPGAKSVPPKPASTAATRAVFPPASHRSPISHEGSLSSAAMSPSFSPSLSPLAARSPVVSPFGVAQGPSASALSAESGLEPPDDTELHI

Target ORF information:

RefSeq Version	XM_002701345.4
Organism	Bos taurus(cattle)
Definition	Bos taurus dachsous cadherin-related 1 (DCHS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002701345.4

ORF Insert Sequence:

ATGCAGAAGG AGTGGGCCAC TGCACCTTCG TGCCCGGGCA TGCAGATCCC CAGGCCTCTC 
CCCCTGCTGT CATTGCTGAT GCTGCTGCTC CTGGGGGCGG GGGCTCCAGG TGCCTGGGGT 
CAGGCTGGGA GCTTGGACCT GCAGATTGAT GAGGAGCAGC CAGCGGGCAC GCTGATCGGG 
GACATCAGTG CAGGGCTTCC AGCGGGCACA GCAGCACCTC CCATGTACTT CATCTCTGCC 
CAGGAGGGCA GCGGGGTGGG CACGGACCTG GCCATCGATG AACACAGTGG GGTGGTCCGT 
ACAGCCCGTG TCTTGGACCG AGAGCGGCGG GATCGCTACC GCTTCACTGC AGTCACTCCC 
GACGGTGCCA CTGTGGAAGT TACCGTGCGA GTGGCTGACA TCAATGACCA TGCTCCGGCT 
TTCCCACAGG CTCGGGCTGT CCTGCAGATA CCTGAGCATA CAGCACTTGG CACCCGCTAC 
CCACTGGAGC CTGCTCGTGA TGCCGACGCT GGCCGCCTGG GAACGCAGGG CTATGCCCTG 
TCTGGTGATG GGGCTGGAGA GACCTTCCGG CTGGAGACAC GCCCTGGTCC AGATGGGGCC 
CCAGTGCCTG AGCTGGTAGT TACCGGAGAG CTGGACCGAG AGAACCGCTC ACACTACATG 
CTGCAACTGG AGGCCTATGA CGGTGGTTCA CCCCCTCGGA GGGCCCAGGC CCTGCTGGAC 
GTGACACTGC TAGACATCAA TGACCATGCC CCAGCTTTCA ATCAGAGCCG CTACCACGCT 
GCTGTGTCCG AGAGCCTGGC CCCCGGCAGT CCTGTCTTGC AGGTGTATGC CTCTGATGCC 
GATGCAGGTG CCAATGGGGC TGTAACTTAT GAGATCAACC GGAGGCAGAG CGAGGGTGAT 
GGACCCTTCT CCATTGATGC ACACACGGGG CTGCTGCGGC TGGAGCGGCC GCTGGACTTT 
GAGCAACGTC GGGTCCACGA ACTGGTGGTG CAGGCTCGGG ATGGAGGGGC TCACCCTGAG 
CTGGGCTCAG CCTTTGTGAC CGTGCATGTG CGAGATGCCA ATGACAATCA GCCCTCCATG 
ACTGTCATCT TCCTGAGTGC AGATGGCTCC CCCCGTGTAT CTGAGGCTGC CCCCCCGGGC 
CAGCTTGTTG CTCGAATCTC TGTGTCAGAC CCAGATGACG GTGACTTTGC GCATGTCAAC 
GTGTCCCTGG AGGGTGGAGA GGGCCACTTT GCGCTCAGCA CCCAGGACAG CGTCATCTAC 
CTGGTGTGTG TGGCTCGGAG GCTGGATCGG GAGGAGCGCG ACGCCTACAA CTTGCGAGTT 
ACTGCCACAG ACTCAGGCTC ACCCCCACTG CGGGCCGAGG CTGCCTTTGT GCTACACGTC 
ACTGATGTCA ATGACAATGC GCCTGCCTTT GACCGCCAGC TCTACCGACC CGAGCCTCTG 
CCTGAGGTTG CCCTGCCTGG CAGCTTCGTG GCGCGGGTGA CGGCCCGGGA TCCTGACCAG 
GGCACCAATG GTCAGGTCAC CTACAGCCTG GCCCCAGGCG CCCACACCCG CTGGTTCTCC 
ATAGACCCCA CCTCAGGCAT TGTCACTACA GCTGCTTTGC TGGACTATGA GTTGGAACCT 
CAGCCACAGC TAATCGTGGT GGCCACGGAT GGGGGCTTGC CCCCTCTCTC CTCCTCTGCC 
ACAGTGAGCG TGGTCTTGCA AGATGTGAAT GATAACGAGC CGCAGTTCCA GAGGACTTTC 
TACAACGCCT CGCTGCCTGA GGGCACTCAG CCAGGAACCT GCTTCCTGCA GGTGACAGCC 
ACAGATGCAG ACAGTGGCCC ATTTGGCCTC CTCTCCTATT CCTTGGGTGC CGGACTTGGG 
ACCTCAGGGT CTCCCCCGTT CCGCATCGAT GCCCACAGTG GTGACGTGTG TACGACCCGG 
ACCCTGGACC GCGATCAGGG GCCCTCAAGC TTTGACTTCA CAGTGACAGC TGTGGATGGG 
GGAGGCCTCA AGTCTATGGT ATATGTGAAG GTGTTTGTGT CAGACGAGAA TGACAACCCA 
CCTCAGTTTT ATCCACGGGA GTATGCTGCC AGTCTGAGCG CCCAGAGTGC ACCAGGCACA 
GCTGTGCTGA GGGTACGTGC CCATGACCCT GATCAGGGAC CCCATGGGCG ACTCTCCTAC 
CACATCCTGG CTGGCAACAG CCCCCCGCTC TTTGCCTTGG ACGAGCACTC AGGACTATTG 
ACAGTAGCCT GGCCCTTGGC CAGACGGGCC AGTTCCATGG TGCAGCTGGA GATCGGGGCT 
CGGGATGGAG GTGGCCTGCA GGCGGAGCCC AATGCCCGAG TCAACATCAG CATTGTGCCT 
GGAACTCCTG TACCACCAGT ATTTGAGCAG CTTCAGTATG TCTTTTCTGT GCCAGAGGAT 
GTGGCACCAG GCACCAGTGT GGGCGTAGTC CAGGCACACA ATCCACCAGG TCGCTTGGGA 
CCGGTGACCC TTGCCCTATC GGGGGGCGAT CCCCGAGGAC TCTTCTCCCT AGATGGGGCC 
ACTGGGCTGT TACAGACACT TCGCCCTCTG GACCGGGAGC TGCTGGGACC AGTGCTGGAG 
CTGGAGGTGC GGGCAGGCAG CGGAGTGCCA CCGGCTTTCG CTGTAGCTCG GGTGCGCGTG 
CTGCTGGACG ATGTGAATGA CAACTCCCCT GCCTTCCCTG CGCCTGAAGA CACAGTGCTG 
CTGCCATCGA GCACTGCCCC AGGGACCCTC ATCTATACAC TGCGGGCTCT GGACCCCGAC 
TCAGGAGTTA ACAGTCGAGT CACCTTTACC CTGCTTGCTG GGGGTGGTGG GACCTTCACT 
GTGGACCCCA CCACAGGCCA CATACGGCTT ATGGGACCTC TGGGGCCTCC GGGGGGGCCA 
GCCCATGAAC TGGAGCTGGA AGCCCGGGAT GGAGGCTCCC CACCTCGCAC CAGCCACTTT 
CGACTGCGGG TGGTGGTACA GGACTTGGGG ACCCGTGGGC TGGCTCCCCA CTTCGACAGC 
CCTACCTACC GTGTAGACCT GCCCTCAGGC ACCACCCCTG GAACTCAAGT CCTACAAGTG 
CAGGCTCGAG CACCAGATGG GAGCCCTGTC ACCTACCACC TTGCAGCAGA TGGGCCAGGT 
AGCCCCTTTG GCCTGGAACC ACAGAGTGGA TGGCTGTGGG TGCGGGCAGC ACTGGACCAT 
GAGGCCCAGG AGTTGTACAC ACTGAAAGTA ATGGCAGTGT CTGGATCCAA AGCTGAGTTG 
GGGCAGCAGA CAGCCACGGC CACCGTGAGG ATCAGCATCC TCAACCAGAA CGACCACAGT 
CCCCGCTTGT CTGAGGAGCC CACCTTCCTG GCTGTGGCTG AGAACCAGCC CCCAGGGACA 
AGCGTGGGTC GGGTCTTTGC CACTGACCGA GATTCAGGAA TCAACGGACG TCTGACCTAC 
AGCCTGCGAC AGCTGTCAGA AGACAGCAAG GCCTTCCGCA TCCACCCCCA GACTGGAGAG 
GTGACCACAC TACAAACTCT GGACCGTGAG CGGCAGAACA GCTACCAGCT TCTGGTGCAG 
GTTCAGGATG CAGGGAGCCC ACCCCGCAGC ACCACAGGCA CCGTGCACAT CGCAGTGCTT 
GACCTCAATG ACAACAGCCC TACCTTCCTG CAGGCCTCAG GGGCTGCTGG TGGAGGCCTC 
CCCATACAGG TACCAGATGG TGTGCCTCCA GGAACATTGG TGACAACCCT GCAGGCCAAG 
GATCCAGATG AGGGGGAGAA TGGAACCATC CTGTACATGC TAACTGGTCC TGGCTCAGAG 
CTCTTCTCCC TGCACCCTCA CTCGGGAGAG CTGCTCACGG CAGCACCCCT GATCCGAGCA 
GAGCGGCCAC ACTACGTGCT CACATTGAGC GCTCATGACC AAGGCAGCCC CCCTCGGAGC 
TCCAGCCTCC AGCTGCTAGT GCAGGTGCTT CCCTCCACTC GCTCGGCTGA GCCTGCACCG 
GAACCCTCAG AGCCAGACCC GGTGGCGCCT GTGCCTGTCG TGCTGACCGT GACAGCAGCC 
GAGGGGCTCC GGCCCGGCTC CCTGTTGGGC TCTGTGGCAC CGCTGGAGCA GGCGGGGGTG 
GGCGCCCTTA CCTACACACT GGTGGGCGGC GCCGACCCAG AGGGCACCTT TGCGCTGGAC 
GCAGCCTCTG GGCGCTTGTA CCTGGCGCGG CCCCTGGACT TCGAGGCGGG TCCGGCTTGG 
CGCGCGCTCA CGGTGCGCGC CGAGGGGCCG GGAGGCGCGG GCGCGCGGCT GCTGAGAGTG 
CAGGTGCGCG TGCAGGACGA GAACGAGCAC GCGCCGGCCT TCGCGCGCGA CCCGCTGGCG 
TTGGCGCTCC CAGAGAACCC GGAGCCGGGC GCGACCTTGT ACACTTTCCG CGCGTCCGAC 
GCCGACGGCC CCGGCCCCAA CAGCGATGTG CGCTACCGCC TGCTGCGCCA AGAGCCGCCT 
GTGCCCGCGC TTCGCCTGGA CGCGCGCACC GGAGCGCTCA GCGCCCCGCG AGGCCTGGAT 
CGGGAGACCA CTCCGGCTCT GCTGCTGCTC GTGGAAGCCA CTGACCGGCC CGCCAACGCC 
AGCCGCCGCC GCACAGCACG CGTTTCCGCG CGCGTCTTTG TCACGGATGA GAATGACAAT 
GCTCCAGTCT TTGCCTCGCC CTCACGTGTG CGCCTCCCTG AGGATCACCC GCCCGGGCCC 
ACGGCGCTGC ACGTGGTAGC CCGGGACCCC GACCTGGGCG AGGCCGCACG GGTGTCCTAT 
CGCCTGGCAG CTGGCGCGGA CGGCTGTTTC CGGCTACACC CCAGCACCGG AGCGCTGTCC 
GTGGTGCGGT CTCTGGACCG CGAACAGCGA GCTGAGTACG TGCTGACTGT GGTGGCCTCT 
GACCACGGCT CCCCACCGCG CTCGGCCACG CAGCTTCTGA CTGTCAGTGT CGCCGACGTC 
AACGACGAGG CACCCGCTTT CCAACAGCAG GAGTACAGCG TCCTCTTGCG GGAGAACAGC 
CCGCCTGGAA CGTCTCTGCT CACTCTGCAG GCAACCGACC CCGATCTGGG GGCCAATGGG 
CAAGTGACTT ATGGAGGCAT CTCTGGCGAA AACTTCTCCC TGGATCCAGA CACCGGGGTC 
CTTACCACTC TTCGGGCTCT GGATCGGGAG GAGCAGGAGG AGATCAACTT GACAGTATAT 
GCCCGGGACG GGGGCTCGCC TCCACTGTTG ACCCACGTCA CAGTGCGAGT GGCTGTGGAG 
GATGAGAATG ACCATGCCCC AACCTTTGGG AGTGCCCATC TCTCCCTGGA GGTGCCCGAG 
GGCCAGGACC CCCAGACCCT TACCACGCTG CGGGCCTCTG ACCCGGATGT GGGAGCCAAC 
GGGCAGCTGC AGTACCGCAT CCTGGATGGG GACCCCTCAG GAGCCTTTGT CCTGGACCTA 
GCTTCTGGGG AGTTTGGCAC CATGAGGCCA CTAGACCGGG AGGTGGAGCC AGCATTCCAG 
CTGCAGATAG AAGCCCGGGA TGGAGGCCAG CCAGCTCTCA GCGCTACGCT TCTTGTGACA 
GTGACAGTGC TAGATGCCAA TGACCATGCC CCAGCCTTCC CTGTGCCCTC CTACTCTGTG 
GAGGTGCCTG AGGACGCACC TGCAGGGACC CTGCTGCTGC AGCTGCAGGC TCATGATCCT 
GATGCTGGGG CCAATGGTCG TGTGACCTAC TACCTGGGTA CAGGTGCAGC AGGAGCCTTC 
CTGCTAGAGC CCAGCTCTGG GGAATTGCGC ACAGCCACCA CCCTTGACAG AGAGCAGTGT 
CCCAGCTATG CCTTTACAGT GAATGCAGTG GACAGTGCAG CTGCTGGGCC CCTAAGCACC 
ACAGTGCCCG TCACCATCAC AGTGCGTGAT GTCAATGACC ACGCGCCCAC CTTCCCCACC 
AGTCCCCTGA GGCTGCGCCT GCCCCGCCCA GGCCCTGTCT TCAGCACTCC AGTTCTGCCT 
CTGGCCACTC TGCGAGCTGA AGACCGTGAC GCTGGTGCCA ATGCCTCCAT CCTGTACCGG 
CTGGCAGGCA CACCACCTCC TGGCACCACC GTGGACTCTT ACACTGGTGA AATCCGTGTG 
TCCCGCTCCC CTGTATCTCT GAGCCCCCGA GATCGTGTCC TCTTCATTGT GGCAACTGAC 
CTTGGCCGTC CAGCTCGCTC TGCCACTGGT GTGGTCATTA TTGGGCTGCA AGGGGAGTCT 
GAGCATGGAC CCCGCTTCTC CCGAGCCAGT AGTGAAGCCA TGCTCCGTGA GAATGCACCC 
CCAGGGACTC CCGTTGTCTC CCCCAAGGCT ATCCATGCAG GGGGCTCAAG TGGACCCATC 
ACATACAGCA TTCTCAGTGG GAATGAGAAA GGGACATTCT CCATCCAGCC TAGTACAGGA 
GCCATCACAG TCCGCTCAGC AGAGGGGCTG GATTTTGAGG CAAACCCACG GCTGCGACTG 
GTGCTGCAGG CAGAGAGTGG AGGAGCCTAT GCCTTCTCTG TGCTGACCCT AACCCTGCAA 
GATGCCAATG ACAACGCGCC CCGCTTCCTG CAGCCCCACT ACGTGGCCTT CCTTCCAGAG 
TCCAGGCCCT TGGAGGGACC CCTGCTACAG GTGGAAGCAG ATGACCTGGA CCAAGGCCCC 
AGTGGACAGA TCTCCTACAG TCTGGCCGCA TCCCAGCCAG CCCGGGGATT GTTCCATGTA 
GACCCAGCCA CGGGCACTAT CACCACCACA GCTATCCTGG ACCGCGAGAT CTGGGCCGAA 
ACACGGCTGG TGCTGATGGC CACAGACAGA GGAAGCCCAG CCCTGGTGGG CTCAGCTACC 
CTGACGGTGA TGGTCATCGA CACCAATGAC AATCGCCCCA CCATCCCCCA GCCCTGGGAG 
CTCCGAGTGT CAGAAGATGC ACTGCTGGGC TCGGAGATCG CACAGGTAAC GGGGAATGAT 
GTGGACTCAG GACCAGTGCT GTGGTACGTT CTGAGCCCTT CTGGGCCCCA GGATCCCTTC 
AGTGTCGGCC GCTATGGAGG CCGCCTCTCC CTCACGGGCC CCTTGGATTT TGAGCAGCGC 
GACCGCTACC ACCTGCAGCT CCTGGCACAC GACGGGCCTC ACGAGGGCCG GGCCAACCTC 
ACGGTGTTCG TGGAGGATGT CAATGACAAC TCCCCTGCCT TCTCACAGAC TCTCTACCAG 
GTGATGCTGC TCGAGCACAC ACCCCCGGGC AGTGCCATTC TCTCCGTCTC GGCCACTGAC 
CGGGACTCGG GCGCCAACGG TCACGTCTCC TACCACCTGG CTTCCTCTGC TGAGGGCTTC 
AGCATTGACC CCAACAACGG GACCCTATTC ACAACAGTGG GGACAGTGGC CTTGGGCCAT 
GAAGGGCCAG GCATGGTGGA CGTGGTGCTG GAAGCCCGAG ACCACGGAGT GCCGGTTCGG 
GCCGCCCGAG CCACAGTGCA CGTGCAGCTG CAGGATCAGA ACGACCATGC CCCGAGCTTC 
ACGCTGCCAC ACTACCGCGT GGCTGTGACT GAGGATCTGC CGCCCGGCTC CACCCTGCTC 
ACCCTGGAGG CCACAGATGC CGACGGGAGC CGCAGCCATG CCGCCGTGGA CTACAGCATT 
GTCAGTGGCA ACCGGGGCCG AGTCTTTCAG CTGGAACCCC GGCTGGCGGA GGCTGGGGAG 
AGTGATGGAC TAGGCCCCCG GGCCCTGGGC TGCCTGGTGT TGCTTGAGCC TCTAGACTTT 
GAAAGCCTAA CCCAGTACAA CCTAACTGTG GCAGCAACTG ACCGGGGGCA GCCACCTCGA 
AGTTCGGCTG TGCCAGTCAC CGTCAGCGTG CTGGATGTCA ACGACAACCC ACCTGTCTTC 
ACCCGAGCAG CCTACCGTGT GGCGGTGCCT GAGGACACGC CTGTGGGAGC CGAGCTGCTG 
CACGTGGAGG CCTCCGACGC TGATCCAGGC CCCCATGGCC TTGTACGCTT CACCCTCAGC 
TCAGGCGACC CTCTGGGGCT CTTTGAGCTG GATGAGAGCT CCGGGGACTT GCGACTGGCC 
CGCCCGTTGG ACTGTGAGAC CCAAGCTCGA CATCAGCTTG TAGTGCAGGC TGCCGACCCT 
GCCGGGGCAC ACTTTGCTCT GGCACCAGTG ACCATTGAGG TCCAGGACGT GAATGACCAT 
GGCCCCGCCT TCCCTCTGAG CTTGCTCAGC ACCAGCCTGG CTGAGAACCA GCCTCCAGGC 
ACTCTTGTTA CCACCCTGCA TGCAATTGAT GGGGATGCTG GGGCTTTCGG AAGGCTCCAC 
TACAGCCTGC TGGAGGCTGG GCCGGGGCCC GAGGGCCGCG AGGCGTTTGC GCTCAACAGC 
TCAACTGGGG AGTTGCGGGC ACGAGTGGCC TTTGACTACG AGCACACTGG CAGCTTCCAG 
CTGCTGGTGG GCGCTACGGA TGCCGGGAAC CTGTCCGCCT CAGTCACTGT GTCAGTGCTG 
GTGACCGGTG AGGATGAGTA TGACCCAGTG TTCCTGGCTC CAGCTTTCCA CTTTCAGGTG 
CCAGAAGGTG CACGGCGTGG CCACAGCCTG GGTCACGTGC AGGCCACAGA CGAGGATGGA 
GGTGCTGACG GCCTGGTGCT GTACTCGCTG GCCGCCTCGT CCCCCTATTT TGGTATCAAC 
CAGACTACAG GTGCCCTGTA CCTGCGGGTG GACAGCCGGG CACCAGGCAG TGGAACAGGC 
ACCTCCGGGG GTGGGGGCCG GACCCGACGT GAGGCGCCAC GGGAGCTGAG GCTGGAGGTG 
GTGGCAAGGG GGCCTCTGCC CGGTTCTCGG AGTGCCACCG TGCCTGTGAC TGTGGACATC 
ACCCACACTG CGCTGGGCCT GGCACCTGAC CTCAACCTGC TCTTGGTGGG AGCCGTGGCA 
GCCTCTCTGG GAGTCGTGGT GGTGCTTGCC CTGGCAGCCC TTGTCCTGGG ACTGGTGCGG 
GCCCGGAGCC GCAAAGCTGA GGCGGCGCCT GGCCCGATGT CCCAGGCGGC ACCCCTGGCC 
AGTGGCTCAC TGCAGAAGCT GGGCCGAGAG CCACCCAGCC CGCCGCCCTC AGAGCACCTG 
TATCACCAGA CCCTCCCCAG CTATGGTGGG CCTGGAGCTG GAGGACCCTA CCCCCGCGGT 
GGCTCCCTGG ACCCTTCACA CTCAAGCGGC CGAGGCTCAG CAGAGGCTGC GGAAGATGAC 
GAGATCCGCA TGATCAACGA GTTCCCCCGC GTGGCCAGTG TGGCCTCCTC CCTGGCTGCC 
CGCGGCCCTG ACTCCGGCAT CCAGCAGGAT GCAGACGGAC TGAGTGACAC CTCCTGTGAG 
CCACCTGCCC CTGACACCTG GTATAAGGGC CGTAAGGCAG GGCTGCTGCT GCCGGGTGCA 
GGAGCCACTC TGTACAGAGA AGACGGCCCC CCAGCCACTG CCACCACCTT CCTGGGGGGC 
TGTGGCCTAA GCCCCGCGCC CACCGGGGAC TACGGCTTTC CAGCAGATGG CAAGCCCTGT 
GTGGCGGGAG CGCTGACGGC CATCGTGGCC GGTGAGGAGG AGCTCCGTGG CAGCTACAAC 
TGGGACTACC TGCTGAGCTG GTGCCCTCAG TTCCAGCCGC TGGCCAGTGT CTTCACAGAG 
ATTGCCCGGC TCAAGGATGA AGCTCGGCCG TGTCCCCCGG CGCCCCGTAT TGACCCACCG 
CCCCTCATCA CTGCCGTGGC CCACCCAGGA GCCAAGTCTG TGCCCCCCAA ACCAGCCAGC 
ACGGCCGCAA CCCGGGCCGT CTTCCCACCA GCCTCTCACC GCTCCCCCAT CAGCCACGAA 
GGTTCCCTGT CCTCGGCGGC CATGTCTCCC AGCTTCTCAC CTTCACTATC TCCTCTGGCC 
GCTCGCTCAC CCGTCGTCTC ACCATTTGGG GTGGCCCAGG GCCCCTCAGC CTCAGCTCTG 
AGCGCAGAGT CTGGCCTGGA GCCACCAGAC GACACCGAGC TCCATATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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DCHS1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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