DCHS1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DCHS1
Entrez Gene ID	8642
Full Name	dachsous cadherin-related 1
Synonyms	CDH19,CDH25,CDHR6,FIB1,MVP2,PCDH16,VMLDS1
General protein information	Preferred Names dachsous cadherin-related 1 Names protocadherin-16 cadherin-19 cadherin-25 cadherin-related family member 6 fibroblast cadherin 1 fibroblast cadherin FIB1 protein dachsous homolog 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.4
Summary	This gene is a member of the cadherin superfamily whose members encode calcium-dependent cell-cell adhesion molecules. The encoded protein has a signal peptide, 27 cadherin repeat domains and a unique cytoplasmic region. This particular cadherin family member is expressed in fibroblasts but not in melanocytes or keratinocytes. The cell-cell adhesion of fibroblasts is thought to be necessary for wound healing. [provided by RefSeq, Jul 2008].
Disorder MIM:	603057
Disorder Html:	Van Maldergem syndrome 1, 601390 (3); Mitral valve prolapse 2, 607829 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_003737.3	NP_003728.1	protocadherin-16 precursor
NM_003737.4	NP_003728.1	protocadherin-16 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DCHS1	XP_003313012.1
Bos taurus (cattle)	DCHS1	XP_002693195.1
Rattus norvegicus (Norway rat)	Dchs1	NP_001101014.2
Homo sapiens (human)	DCHS1	NP_003728.1
Danio rerio (zebrafish)	dchs1b	XP_001921284.3
	DCHS1	XP_001100797.1
Mus musculus (house mouse)	Dchs1	NP_001156415.1
Gallus gallus (chicken)	DCHS1	XP_417264.4
Caenorhabditis elegans (roundworm)	cdh-1	NP_497641.2
Canis lupus familiaris (dog)	DCHS1	XP_853733.2
Drosophila melanogaster (fruit fly)	ds	NP_523446.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007905	XP_317578.4
Danio rerio (zebrafish)	LOC100334632	XP_002664697.2

Gene Ontology
Bibliography

Related articles in PubMed

Deleterious variants in DCHS1 are prevalent in sporadic cases of mitral valve prolapse.
Clemenceau A, B?rub? JC, B?langer P, Gaudreault N, Lamontagne M, Toubal O, Clavel MA, Capoulade R, Mathieu P, Pibarot P, Bosse Y
Molecular genetics & genomic medicine(2017 Dec)

Mammalian cadherins DCHS1-FAT4 affect functional cerebral architecture.
Beste C, Ocklenburg S, von der Hagen M, Di Donato N
Brain structure & function221(5)2487-91(2016 06)

The atypical cadherin Dachsous1 localizes to the base of the ciliary apparatus in airway epithelia.
Dau C, Fliegauf M, Omran H, Schlensog M, Dahl E, van Roeyen CR, Kriz W, Moeller MJ, Braun GS
Biochemical and biophysical research communications473(4)1177-1184(2016 05)

Mutations in DCHS1 cause mitral valve prolapse.
Durst R, Sauls K, Peal DS, deVlaming A, Toomer K, Leyne M, Salani M, Talkowski ME, Brand H, Perrocheau M, Simpson C, Jett C, Stone MR, Charles F, Chiang C, Lynch SN, Bouatia-Naji N, Delling FN, Freed LA, Tribouilloy C, Le Tourneau T, LeMarec H, Fernandez-Friera L, Solis J, Trujillano D, Ossowski S, Estivill X, Dina C, Bruneval P, Chester A, Schott JJ, Irvine KD, Mao Y, Wessels A, Motiwala T, Puceat M, Tsukasaki Y, Menick DR, Kasiganesan H, Nie X, Broome AM, Williams K, Johnson A, Markwald RR, Jeunemaitre X, Hagege A, Levine RA, Milan DJ, Norris RA, Slaugenhaupt SA
Nature525(7567)109-13(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study shows that when key regulators during mammalian cerebral cortical development are disrupted due to DCHS1-FAT4 mutations, functional cerebral asymmetries are stronger.
Title: Mammalian cadherins DCHS1-FAT4 affect functional cerebral architecture.

In sum, we establish Dchs1 as a component of the membrane domain surrounding the ciliary base. This suggests a specific role of Dchs1 in PCP-dependent organization of ciliary function and a possible role in lung disease.
Title: The atypical cadherin Dachsous1 localizes to the base of the ciliary apparatus in airway epithelia.

DCHS1 deficiency in mitral valve prolapse patient mitral valve interstitial cells (MVICs), as well as in Dchs1(+/-) mouse MVICs, result in altered migration and cellular patterning, supporting these processes as aetiological underpinnings for the disease
Title: Mutations in DCHS1 cause mitral valve prolapse.

These findings suggest that Fat and Dachsous self-bend due to the loss of Ca(2+)-binding amino acids from specific EC-EC linkers, and can therefore adapt to confined spaces.
Title: Giant cadherins Fat and Dachsous self-bend to organize properly spaced intercellular junctions.

findings show that mutations in genes encoding the receptor-ligand cadherin pair DCHS1 and FAT4 lead to a recessive syndrome in humans that includes periventricular neuronal heterotopia
Title: Mutations in genes encoding the cadherin receptor-ligand pair DCHS1 and FAT4 disrupt cerebral cortical development.

The following DCHS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DCHS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21465	NM_003737.4 Latest version!	Homo sapiens dachsous cadherin-related 1 (DCHS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OHu21465	NM_003737.3	Homo sapiens dachsous cadherin-related 1 (DCHS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DCHS1 ( NM_003737.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003728.1 Homo sapiens dachsous cadherin-related 1 (DCHS1), mRNA.

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CloneID	OHu21465
Clone ID Related Accession (Same CDS sequence)	NM_003737.3 , NM_003737.4
Accession Version	NM_003737.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-24
Organism	Homo sapiens(human)
Product	protocadherin-16 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC091564.12, AB053446.1 and AI767189.1. This sequence is a reference standard in the RefSeqGene project. On Dec 10, 2013 this sequence version replaced NM_003737.2. ##Evidence-Data-START## Transcript exon combination :: AB053446.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGAAGG AGCTGGGCAT TGTGCCTTCC TGCCCTGGCA TGAAGAGCCC CAGGCCCCAC 
CTCCTGCTAC CATTGCTGCT GCTGCTGCTG CTGCTGCTGG GGGCTGGGGT GCCAGGTGCC 
TGGGGTCAGG CTGGGAGCCT GGACTTGCAG ATTGATGAGG AGCAGCCAGC GGGTACACTG 
ATTGGCGACA TCAGTGCGGG GCTTCCGGCA GGCACGGCAG CTCCTCTCAT GTACTTCATC 
TCTGCCCAAG AGGGCAGCGG CGTGGGCACA GACCTGGCCA TTGACGAACA CAGTGGGGTC 
GTCCGTACAG CCCGTGTCTT GGACCGTGAG CAGCGGGACC GCTACCGCTT CACTGCAGTC 
ACTCCTGATG GTGCCACCGT AGAAGTTACA GTGCGAGTGG CTGACATCAA CGACCATGCT 
CCAGCCTTCC CACAGGCTCG GGCTGCCCTG CAGGTACCTG AGCATACAGC TTTTGGCACC 
CGCTACCCAC TGGAGCCTGC TCGTGATGCA GATGCTGGGC GTCTGGGAAC CCAGGGCTAT 
GCGCTATCTG GTGATGGGGC TGGAGAGACC TTCCGGCTGG AGACACGCCC CGGTCCAGAT 
GGGACTCCAG TACCTGAGCT GGTAGTTACT GGGGAACTGG ACCGAGAGAA CCGCTCACAC 
TATATGCTAC AGCTGGAGGC CTATGATGGT GGTTCACCCC CCCGGAGGGC CCAGGCCCTG 
CTGGACGTGA CACTGCTGGA CATCAATGAC CATGCCCCGG CTTTCAATCA GAGCCGCTAC 
CATGCTGTGG TGTCTGAGAG CCTGGCCCCT GGCAGTCCTG TCTTGCAGGT GTTCGCATCT 
GATGCCGATG CTGGTGTCAA TGGGGCTGTG ACTTACGAGA TCAACCGGAG GCAGAGCGAG 
GGTGATGGAC CCTTCTCCAT CGACGCACAC ACGGGGCTGC TGCAGTTAGA GCGGCCACTG 
GACTTTGAGC AGCGGCGGGT CCATGAACTG GTGGTGCAAG CACGAGATGG TGGGGCTCAC 
CCTGAGCTGG GCTCGGCCTT TGTGACTGTG CATGTGCGAG ATGCCAATGA CAATCAGCCC 
TCCATGACTG TCATCTTTCT CAGTGCAGAT GGCTCCCCCC AAGTGTCTGA GGCCGCCCCA 
CCTGGACAGC TCGTTGCTCG CATCTCTGTG TCAGACCCAG ATGATGGTGA CTTTGCCCAT 
GTCAATGTGT CCCTGGAAGG TGGAGAGGGC CACTTTGCCC TAAGCACCCA AGACAGCGTC 
ATCTATCTGG TGTGTGTGGC TCGGCGGCTG GATCGAGAGG AGAGGGATGC CTATAACTTG 
AGGGTTACAG CCACAGACTC AGGCTCACCT CCACTGCGGG CTGAGGCTGC CTTTGTGCTG 
CACGTCACTG ATGTCAACGA CAATGCACCT GCCTTTGACC GCCAGCTCTA CCGACCTGAG 
CCCCTGCCTG AGGTTGCGCT GCCTGGCAGC TTTGTAGTGC GGGTGACTGC TCGGGATCCT 
GACCAAGGCA CCAATGGTCA GGTCACTTAT AGCCTAGCCC CTGGCGCCCA CACCCACTGG 
TTCTCCATTG ACCCCACCTC AGGCATTATC ACTACGGCTG CCTCACTGGA CTATGAGTTG 
GAACCTCAGC CACAGCTGAT TGTGGTGGCC ACAGATGGTG GCCTGCCCCC TCTAGCCTCC 
TCTGCCACAG TTAGCGTGGC CCTGCAAGAT GTGAATGATA ATGAGCCCCA ATTCCAGAGG 
ACTTTCTACA ATGCCTCACT GCCTGAGGGC ACCCAGCCTG GAACTTGCTT CCTGCAGGTG 
ACAGCCACAG ACGCGGATAG TGGCCCATTT GGCCTCCTCT CCTATTCCTT GGGTGCTGGA 
CTTGGGTCCT CCGGATCTCC CCCATTCCGC ATTGATGCCC ACAGCGGTGA TGTGTGCACA 
ACCCGGACCC TGGACCGTGA CCAGGGGCCC TCAAGCTTTG ACTTCACAGT GACAGCTGTG 
GATGGGGGAG GCCTCAAGTC CATGGTATAT GTGAAGGTGT TTCTGTCAGA CGAGAATGAC 
AACCCTCCTC AGTTTTATCC ACGGGAGTAT GCTGCCAGTA TAAGTGCCCA GAGTCCACCA 
GGCACAGCTG TGCTGAGGTT GCGTGCCCAT GACCCTGACC AGGGATCCCA TGGGCGACTC 
TCCTACCATA TCCTGGCTGG CAACAGCCCC CCACTTTTTA CCTTGGATGA GCAATCAGGG 
CTGTTGACAG TAGCCTGGCC CTTGGCCAGA CGGGCCAATT CTGTGGTGCA GCTGGAGATC 
GGGGCTGAGG ACGGAGGTGG CCTACAGGCA GAACCCAGTG CCCGAGTGGA CATCAGCATT 
GTGCCTGGAA CCCCCACACC ACCCATATTT GAGCAACTAC AGTATGTTTT TTCTGTGCCA 
GAGGATGTGG CACCAGGCAC CAGTGTGGGC ATAGTCCAGG CACACAACCC ACCAGGTCGC 
TTGGCACCTG TGACCCTTTC CCTATCAGGT GGGGATCCCC GAGGACTCTT CTCCCTAGAT 
GCGGTATCAG GACTGTTGCA AACACTTCGC CCTCTGGACC GGGAGCTACT GGGACCAGTG 
TTGGAGCTGG AGGTGCGAGC AGGCAGTGGA GTGCCCCCAG CTTTCGCTGT AGCTCGGGTG 
CGTGTGCTGC TGGATGATGT GAATGACAAC TCCCCTGCCT TTCCTGCACC TGAAGACACG 
GTATTGCTAC CACCAAACAC TGCCCCAGGG ACTCCCATCT ATACACTGCG GGCTCTTGAC 
CCCGACTCAG GTGTTAACAG TCGAGTCACC TTTACCCTGC TTGCTGGGGG TGGTGGAGCC 
TTCACCGTGG ACCCCACCAC AGGCCATGTA CGGCTTATGA GGCCTCTGGG GCCCTCAGGA 
GGGCCAGCCC ATGAGCTGGA GCTGGAGGCC CGGGATGGGG GCTCCCCACC ACGCACCAGC 
CACTTTCGAC TACGGGTGGT GGTACAGGAT GTGGGAACCC GTGGGCTGGC TCCCCGATTC 
AACAGCCCTA CCTACCGTGT GGACCTGCCC TCAGGCACCA CTGCTGGAAC TCAGGTCCTG 
CAAGTGCAGG CCCAAGCACC AGATGGGGGC CCTATCACCT ATCACCTTGC AGCAGAGGGA 
GCAAGTAGCC CCTTTGGCCT GGAGCCACAG AGTGGGTGGC TATGGGTGCG GGCAGCACTA 
GACCGTGAGG CCCAGGAATT GTACATACTG AAGGTAATGG CAGTGTCTGG GTCCAAAGCT 
GAGTTGGGGC AGCAGACAGG CACAGCCACC GTGAGGGTCA GCATCCTCAA CCAGAATGAA 
CACAGTCCCC GCTTGTCTGA GGATCCCACC TTCCTGGCTG TGGCTGAGAA CCAGCCCCCA 
GGGACCAGCG TGGGCCGAGT CTTTGCCACT GACCGAGACT CAGGACCCAA TGGACGTCTG 
ACCTACAGCC TGCAACAGCT GTCTGAAGAC AGCAAGGCCT TCCGCATCCA CCCCCAGACT 
GGAGAAGTGA CCACACTCCA AACCCTGGAC CGTGAGCAGC AGAGCAGCTA TCAGCTCCTG 
GTGCAGGTGC AGGATGGAGG GAGCCCACCC CGCAGCACCA CAGGCACTGT GCATGTTGCA 
GTGCTTGACC TCAACGACAA CAGCCCCACG TTCCTGCAGG CTTCAGGAGC TGCTGGTGGG 
GGCCTCCCTA TACAGGTACC AGACCGCGTG CCTCCGGGAA CACTGGTGAC GACTCTGCAG 
GCGAAGGATC CAGATGAGGG GGAGAATGGG ACCATCTTGT ACACGCTAAC TGGTCCTGGC 
TCAGAGCTTT TCTCTCTGCA CCCTCACTCA GGGGAGCTGC TCACTGCAGC TCCCCTGATC 
CGAGCAGAGC GGCCCCACTA TGTGCTGACA CTGAGTGCTC ATGACCAAGG CAGCCCTCCT 
CGAAGTGCCA GCCTCCAGCT GCTGGTGCAG GTGCTTCCCT CAGCTCGCTT GGCCGAGCCG 
CCCCCAGATC TCGCAGAGCG GGACCCAGCG GCACCAGTGC CTGTCGTGCT GACGGTGACA 
GCAGCTGAGG GACTGCGGCC CGGCTCTCTG TTGGGCTCGG TGGCAGCGCC AGAGCCCGCG 
GGTGTGGGTG CACTCACCTA CACACTGGTG GGCGGTGCCG ATCCCGAGGG CACCTTCGCG 
CTGGATGCGG CCTCAGGGCG CTTGTACCTG GCGCGGCCCC TGGACTTCGA AGCTGGCCCG 
CCGTGGCGCG CGCTTACGGT ACGCGCTGAG GGGCCGGGAG GCGCGGGCGC GCGGCTGCTG 
CGAGTGCAGG TGCAAGTGCA GGACGAGAAT GAGCATGCGC CCGCCTTTGC GCGCGACCCG 
CTGGCGCTGG CGCTGCCAGA GAACCCGGAG CCCGGCGCAG CGCTGTACAC TTTCCGCGCG 
TCGGACGCCG ACGGCCCCGG CCCCAATAGC GACGTGCGCT ACCGCCTGCT GCGCCAGGAG 
CCGCCCGTGC CGGCGCTTCG CCTGGACGCG CGCACCGGGG CGCTCAGCGC TCCGCGCGGC 
CTGGACCGAG AGACCACTCC CGCGCTGCTG CTGCTGGTGG AAGCCACCGA CCGGCCCGCC 
AACGCCAGCC GCCGTCGTGC AGCGCGCGTT TCAGCGCGCG TCTTCGTCAC GGATGAGAAT 
GACAACGCGC CTGTCTTCGC CTCGCCGTCA CGCGTGCGCC TCCCAGAGGA CCAGCCGCCT 
GGGCCCGCGG CCCTGCACGT GGTAGCCCGG GACCCGGATC TGGGCGAGGC TGCACGCGTG 
TCCTATCGGC TGGCATCTGG CGGGGACGGC CACTTCCGGC TGCACTCAAG CACTGGAGCG 
CTGTCCGTGG TGCGGCCGTT GGACCGCGAA CAACGAGCTG AGCACGTACT GACAGTGGTG 
GCCTCAGACC ACGGCTCCCC GCCGCGCTCG GCCACGCAGG TCCTGACCGT CAGTGTCGCT 
GACGTCAACG ACGAGGCGCC TACTTTCCAG CAGCAGGAGT ACAGCGTCCT CTTGCGTGAG 
AACAACCCTC CTGGCACATC TCTGCTCACC CTGCGAGCAA CCGACCCCGA CGTGGGGGCC 
AACGGGCAAG TGACTTATGG AGGCGTCTCT AGCGAAAGCT TTTCTCTGGA TCCTGACACT 
GGTGTTCTCA CGACTCTTCG GGCCCTGGAT CGAGAGGAAC AGGAGGAGAT CAACCTGACA 
GTGTATGCCC AGGACAGGGG CTCACCTCCT CAGTTAACGC ATGTCACTGT TCGAGTGGCT 
GTGGAGGATG AGAATGACCA TGCACCAACC TTTGGGAGTG CCCATCTCTC TCTGGAGGTG 
CCTGAGGGCC AGGACCCCCA GACCCTTACC ATGCTTCGGG CCTCTGATCC AGATGTGGGA 
GCCAATGGGC AGTTGCAGTA CCGCATCCTA GATGGGGACC CATCAGGAGC CTTTGTCCTA 
GACCTTGCTT CTGGAGAGTT TGGCACCATG CGGCCACTAG ACAGAGAAGT GGAGCCAGCT 
TTCCAGCTGA GGATAGAGGC CCGGGATGGA GGCCAGCCAG CTCTCAGTGC CACGCTGCTT 
TTGACAGTGA CAGTGCTGGA TGCCAATGAC CATGCTCCAG CCTTTCCTGT GCCTGCCTAC 
TCGGTGGAGG TGCCGGAGGA TGTGCCTGCA GGGACCCTGC TGCTGCAGCT ACAGGCTCAT 
GACCCTGATG CTGGAGCTAA TGGCCATGTG ACCTACTACC TGGGCGCCGG TACAGCAGGA 
GCCTTCCTGC TGGAGCCCAG CTCTGGAGAA CTGCGCACAG CTGCAGCCTT GGACAGAGAA 
CAGTGTCCCA GCTACACCTT TTCTGTGAGT GCAGTGGATG GTGCAGCTGC TGGGCCCCTA 
AGCACCACAG TGTCTGTCAC CATCACGGTG CGCGATGTCA ATGACCATGC ACCCACCTTC 
CCCACCAGTC CTCTGCGCCT ACGTCTGCCC CGCCCAGGCC CCAGCTTCAG TACCCCAACC 
CTGGCTCTGG CCACACTGAG AGCTGAAGAT CGTGATGCTG GTGCCAATGC TTCCATTCTG 
TACCGGCTGG CAGGCACACC ACCTCCTGGC ACTACTGTGG ACTCTTACAC TGGTGAAATC 
CGCGTGGCCC GCTCTCCTGT AGCTCTAGGC CCCCGAGATC GTGTCCTCTT CATTGTGGCC 
ACTGATCTTG GCCGTCCAGC TCGCTCTGCC ACTGGTGTGA TCATTGTTGG ACTGCAGGGG 
GAAGCTGAGC GTGGACCCCG CTTTCCCCGG GCTAGCAGTG AGGCTACGAT TCGTGAGAAT 
GCGCCCCCAG GGACTCCTAT TGTCTCCCCC AGGGCCGTCC ATGCAGGAGG CACAAATGGA 
CCCATCACCT ACAGCATTCT CAGTGGGAAT GAGAAAGGGA CATTCTCCAT CCAGCCTAGT 
ACAGGTGCCA TCACAGTTCG CTCAGCAGAG GGGCTAGACT TCGAGGTGAG TCCACGGCTG 
CGACTGGTGC TGCAGGCAGA GAGTGGAGGA GCCTTTGCCT TCACTGTGCT GACCCTGACC 
CTGCAAGATG CCAACGACAA TGCTCCCCGT TTCCTGCGGC CCCATTATGT GGCCTTCCTT 
CCTGAGTCCC GGCCCTTGGA GGGGCCCCTG CTGCAGGTGG AGGCGGATGA CCTGGATCAA 
GGCTCTGGAG GACAGATTTC CTACAGTCTG GCTGCATCCC AGCCGGCACG TGGATTGTTC 
CACGTAGACC CAACCACAGG CACTATCACT ACCACAGCCA TCCTGGACCG TGAGATCTGG 
GCTGAAACAC GGTTGGTGCT GATGGCCACA GACAGAGGGA GCCCAGCCCT GGTGGGCTCA 
GCTACCTTGA CGGTGATGGT CATCGACACC AATGACAATC GCCCCACCAT CCCCCAACCC 
TGGGAGCTCC GAGTGTCAGA AGATGCGTTA TTGGGCTCAG AGATTGCACA GGTAACAGGG 
AATGATGTGG ACTCAGGACC CGTGCTGTGG TATGTGCTAA GCCCATCTGG GCCCCAGGAT 
CCCTTCAGTG TTGGCCGCTA TGGAGGCCGT GTCTCCCTCA CGGGGCCCCT GGACTTTGAG 
CAGTGTGACC GCTACCAGCT GCAGCTGCTG GCACATGATG GGCCTCATGA GGGCCGTGCC 
AACCTCACAG TGCTTGTGGA GGATGTCAAT GACAATGCAC CTGCCTTCTC ACAGAGCCTC 
TACCAGGTAA TGCTGCTTGA GCACACACCC CCAGGCAGTG CCATTCTCTC CGTCTCTGCC 
ACTGATCGGG ACTCAGGTGC CAACGGTCAC ATTTCCTACC ACCTGGCTTC CCCTGCCGAT 
GGCTTCAGTG TTGACCCCAA CAATGGGACC CTGTTCACAA TAGTGGGAAC AGTGGCCTTG 
GGCCATGACG GGTCAGGAGC AGTGGATGTG GTGCTGGAAG CACGAGACCA CGGGGCTCCA 
GGCCGGGCAG CACGAGCCAC AGTGCACGTG CAGCTGCAGG ACCAGAACGA CCACGCCCCG 
AGCTTCACAT TGTCACACTA CCGTGTGGCT GTGACTGAAG ACCTGCCCCC TGGCTCCACT 
CTGCTCACCC TGGAGGCTAC AGATGCTGAT GGAAGCCGCA GCCATGCCGC TGTGGACTAC 
AGCATCATCA GTGGCAACTG GGGCCGAGTC TTCCAGCTGG AACCCAGGCT GGCTGAGGCT 
GGGGAGAGTG CTGGACCAGG CCCCCGGGCA CTGGGCTGCC TGGTGTTGCT TGAACCTCTA 
GACTTTGAAA GCCTGACACA GTACAATCTA ACAGTGGCTG CAGCTGACCG TGGGCAGCCA 
CCCCAAAGCT CAGTCGTGCC AGTCACTGTC ACTGTACTAG ATGTCAATGA CAACCCACCT 
GTCTTTACCC GAGCATCCTA CCGTGTGACA GTACCTGAGG ACACACCTGT TGGAGCTGAG 
CTGCTGCATG TAGAGGCCTC TGACGCTGAC CCTGGCCCTC ATGGCCTCGT GCGTTTCACT 
GTCAGCTCAG GCGACCCATC AGGGCTCTTT GAGCTGGATG AGAGCTCAGG CACCTTGCGA 
CTGGCCCATG CCCTGGACTG TGAGACCCAG GCTCGACATC AGCTTGTAGT ACAGGCTGCT 
GACCCTGCTG GTGCACACTT TGCTTTGGCA CCAGTGACAA TTGAGGTCCA GGATGTGAAT 
GATCATGGCC CAGCCTTCCC ACTGAACTTA CTCAGCACCA GCGTGGCCGA GAATCAGCCT 
CCAGGCACTC TCGTGACCAC TCTGCATGCA ATCGACGGGG ATGCTGGGGC TTTTGGGAGG 
CTCCGTTACA GCCTGTTGGA GGCTGGGCCA GGACCTGAGG GCCGTGAGGC ATTTGCACTG 
AACAGCTCAA CAGGGGAGTT GCGTGCGCGA GTGCCCTTTG ACTATGAGCA CACAGAAAGC 
TTCCGGCTGC TGGTGGGTGC TGCTGATGCT GGGAATCTCT CAGCCTCTGT CACTGTGTCG 
GTGCTAGTGA CTGGAGAGGA TGAGTATGAC CCTGTATTTC TGGCACCAGC TTTCCACTTC 
CAAGTGCCCG AAGGTGCCCG GCGTGGCCAC AGCTTGGGTC ACGTGCAGGC CACAGATGAG 
GATGGGGGTG CCGATGGCCT GGTTCTGTAT TCCCTTGCCA CCTCTTCCCC CTATTTTGGT 
ATTAACCAGA CTACAGGAGC CCTGTACCTG CGGGTGGACA GTCGGGCACC AGGCAGCGGA 
ACAGCCACCT CTGGGGGTGG GGGCCGGACC CGGCGGGAAG CACCACGGGA GCTGAGGCTG 
GAGGTGATAG CACGGGGGCC TCTGCCTGGT TCCCGGAGTG CCACAGTGCC TGTGACCGTG 
GATATCACCC ACACCGCACT GGGCCTGGCA CCTGACCTCA ACCTGCTATT AGTAGGGGCC 
GTGGCAGCCT CCTTGGGAGT TGTGGTGGTG CTTGCACTGG CAGCCCTGGT CCTAGGACTT 
GTTCGGGCCC GTAGCCGCAA GGCTGAGGCA GCCCCTGGCC CAATGTCACA GGCAGCACCC 
CTAGCCAGTG ACTCACTGCA GAAACTGGGC CGGGAGCCAC CTAGTCCACC ACCCTCTGAG 
CACCTCTATC ACCAGACTCT TCCCAGCTAT GGTGGGCCAG GAGCTGGAGG ACCCTACCCC 
CGTGGTGGCT CCTTGGACCC TTCACATTCA AGTGGCCGAG GATCAGCAGA GGCTGCAGAG 
GATGATGAGA TCCGCATGAT CAATGAGTTC CCCCGTGTGG CCAGTGTGGC CTCCTCTCTG 
GCTGCCCGTG GCCCTGACTC AGGCATCCAG CAGGATGCAG ATGGTCTGAG TGACACATCC 
TGCGAACCAC CTGCCCCTGA CACCTGGTAT AAGGGCCGAA AGGCAGGGCT GCTGCTGCCA 
GGTGCAGGAG CCACTCTCTA CAGAGAGGAG GGGCCCCCAG CCACTGCCAC AGCCTTCCTG 
GGGGGCTGTG GCCTGAGCCC TGCACCCACT GGGGACTATG GCTTCCCAGC AGATGGCAAG 
CCATGTGTGG CAGGTGCGCT GACAGCCATT GTGGCCGGCG AGGAGGAGCT CCGTGGCAGC 
TATAACTGGG ACTACCTGCT GAGCTGGTGC CCTCAGTTCC AACCACTGGC CAGTGTCTTC 
ACAGAGATCG CTCGGCTCAA GGATGAAGCT CGGCCATGTC CCCCAGCTCC CCGTATCGAC 
CCACCACCCC TCATCACTGC CGTGGCCCAC CCAGGAGCCA AGTCTGTGCC CCCCAAGCCA 
GCAAACACAG CTGCAGCCCG GGCCATCTTC CCACCAGCTT CTCACCGCTC CCCCATCAGC 
CATGAAGGCT CCCTGTCCTC AGCTGCCATG TCCCCCAGCT TCTCACCCTC TCTGTCTCCT 
CTGGCTGCTC GCTCACCCGT TGTCTCACCA TTTGGGGTGG CCCAGGGTCC CTCAGCCTCA 
GCACTCAGCG CAGAGTCTGG CCTGGAGCCA CCTGATGACA CGGAGCTGCA CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003728.1
CDS	408..10304
Translation	MQKELGIVPSCPGMKSPRPHLLLPLLLLLLLLLGAGVPGAWGQAGSLDLQIDEEQPAGTLIGDISAGLPAGTAAPLMYFISAQEGSGVGTDLAIDEHSGVVRTARVLDREQRDRYRFTAVTPDGATVEVTVRVADINDHAPAFPQARAALQVPEHTAFGTRYPLEPARDADAGRLGTQGYALSGDGAGETFRLETRPGPDGTPVPELVVTGELDRENRSHYMLQLEAYDGGSPPRRAQALLDVTLLDINDHAPAFNQSRYHAVVSESLAPGSPVLQVFASDADAGVNGAVTYEINRRQSEGDGPFSIDAHTGLLQLERPLDFEQRRVHELVVQARDGGAHPELGSAFVTVHVRDANDNQPSMTVIFLSADGSPQVSEAAPPGQLVARISVSDPDDGDFAHVNVSLEGGEGHFALSTQDSVIYLVCVARRLDREERDAYNLRVTATDSGSPPLRAEAAFVLHVTDVNDNAPAFDRQLYRPEPLPEVALPGSFVVRVTARDPDQGTNGQVTYSLAPGAHTHWFSIDPTSGIITTAASLDYELEPQPQLIVVATDGGLPPLASSATVSVALQDVNDNEPQFQRTFYNASLPEGTQPGTCFLQVTATDADSGPFGLLSYSLGAGLGSSGSPPFRIDAHSGDVCTTRTLDRDQGPSSFDFTVTAVDGGGLKSMVYVKVFLSDENDNPPQFYPREYAASISAQSPPGTAVLRLRAHDPDQGSHGRLSYHILAGNSPPLFTLDEQSGLLTVAWPLARRANSVVQLEIGAEDGGGLQAEPSARVDISIVPGTPTPPIFEQLQYVFSVPEDVAPGTSVGIVQAHNPPGRLAPVTLSLSGGDPRGLFSLDAVSGLLQTLRPLDRELLGPVLELEVRAGSGVPPAFAVARVRVLLDDVNDNSPAFPAPEDTVLLPPNTAPGTPIYTLRALDPDSGVNSRVTFTLLAGGGGAFTVDPTTGHVRLMRPLGPSGGPAHELELEARDGGSPPRTSHFRLRVVVQDVGTRGLAPRFNSPTYRVDLPSGTTAGTQVLQVQAQAPDGGPITYHLAAEGASSPFGLEPQSGWLWVRAALDREAQELYILKVMAVSGSKAELGQQTGTATVRVSILNQNEHSPRLSEDPTFLAVAENQPPGTSVGRVFATDRDSGPNGRLTYSLQQLSEDSKAFRIHPQTGEVTTLQTLDREQQSSYQLLVQVQDGGSPPRSTTGTVHVAVLDLNDNSPTFLQASGAAGGGLPIQVPDRVPPGTLVTTLQAKDPDEGENGTILYTLTGPGSELFSLHPHSGELLTAAPLIRAERPHYVLTLSAHDQGSPPRSASLQLLVQVLPSARLAEPPPDLAERDPAAPVPVVLTVTAAEGLRPGSLLGSVAAPEPAGVGALTYTLVGGADPEGTFALDAASGRLYLARPLDFEAGPPWRALTVRAEGPGGAGARLLRVQVQVQDENEHAPAFARDPLALALPENPEPGAALYTFRASDADGPGPNSDVRYRLLRQEPPVPALRLDARTGALSAPRGLDRETTPALLLLVEATDRPANASRRRAARVSARVFVTDENDNAPVFASPSRVRLPEDQPPGPAALHVVARDPDLGEAARVSYRLASGGDGHFRLHSSTGALSVVRPLDREQRAEHVLTVVASDHGSPPRSATQVLTVSVADVNDEAPTFQQQEYSVLLRENNPPGTSLLTLRATDPDVGANGQVTYGGVSSESFSLDPDTGVLTTLRALDREEQEEINLTVYAQDRGSPPQLTHVTVRVAVEDENDHAPTFGSAHLSLEVPEGQDPQTLTMLRASDPDVGANGQLQYRILDGDPSGAFVLDLASGEFGTMRPLDREVEPAFQLRIEARDGGQPALSATLLLTVTVLDANDHAPAFPVPAYSVEVPEDVPAGTLLLQLQAHDPDAGANGHVTYYLGAGTAGAFLLEPSSGELRTAAALDREQCPSYTFSVSAVDGAAAGPLSTTVSVTITVRDVNDHAPTFPTSPLRLRLPRPGPSFSTPTLALATLRAEDRDAGANASILYRLAGTPPPGTTVDSYTGEIRVARSPVALGPRDRVLFIVATDLGRPARSATGVIIVGLQGEAERGPRFPRASSEATIRENAPPGTPIVSPRAVHAGGTNGPITYSILSGNEKGTFSIQPSTGAITVRSAEGLDFEVSPRLRLVLQAESGGAFAFTVLTLTLQDANDNAPRFLRPHYVAFLPESRPLEGPLLQVEADDLDQGSGGQISYSLAASQPARGLFHVDPTTGTITTTAILDREIWAETRLVLMATDRGSPALVGSATLTVMVIDTNDNRPTIPQPWELRVSEDALLGSEIAQVTGNDVDSGPVLWYVLSPSGPQDPFSVGRYGGRVSLTGPLDFEQCDRYQLQLLAHDGPHEGRANLTVLVEDVNDNAPAFSQSLYQVMLLEHTPPGSAILSVSATDRDSGANGHISYHLASPADGFSVDPNNGTLFTIVGTVALGHDGSGAVDVVLEARDHGAPGRAARATVHVQLQDQNDHAPSFTLSHYRVAVTEDLPPGSTLLTLEATDADGSRSHAAVDYSIISGNWGRVFQLEPRLAEAGESAGPGPRALGCLVLLEPLDFESLTQYNLTVAAADRGQPPQSSVVPVTVTVLDVNDNPPVFTRASYRVTVPEDTPVGAELLHVEASDADPGPHGLVRFTVSSGDPSGLFELDESSGTLRLAHALDCETQARHQLVVQAADPAGAHFALAPVTIEVQDVNDHGPAFPLNLLSTSVAENQPPGTLVTTLHAIDGDAGAFGRLRYSLLEAGPGPEGREAFALNSSTGELRARVPFDYEHTESFRLLVGAADAGNLSASVTVSVLVTGEDEYDPVFLAPAFHFQVPEGARRGHSLGHVQATDEDGGADGLVLYSLATSSPYFGINQTTGALYLRVDSRAPGSGTATSGGGGRTRREAPRELRLEVIARGPLPGSRSATVPVTVDITHTALGLAPDLNLLLVGAVAASLGVVVVLALAALVLGLVRARSRKAEAAPGPMSQAAPLASDSLQKLGREPPSPPPSEHLYHQTLPSYGGPGAGGPYPRGGSLDPSHSSGRGSAEAAEDDEIRMINEFPRVASVASSLAARGPDSGIQQDADGLSDTSCEPPAPDTWYKGRKAGLLLPGAGATLYREEGPPATATAFLGGCGLSPAPTGDYGFPADGKPCVAGALTAIVAGEEELRGSYNWDYLLSWCPQFQPLASVFTEIARLKDEARPCPPAPRIDPPPLITAVAHPGAKSVPPKPANTAAARAIFPPASHRSPISHEGSLSSAAMSPSFSPSLSPLAARSPVVSPFGVAQGPSASALSAESGLEPPDDTELHI

Target ORF information:

RefSeq Version	NM_003737.3
Organism	Homo sapiens(human)
Definition	Homo sapiens dachsous cadherin-related 1 (DCHS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003737.3

ORF Insert Sequence:

ATGCAGAAGG AGCTGGGCAT TGTGCCTTCC TGCCCTGGCA TGAAGAGCCC CAGGCCCCAC 
CTCCTGCTAC CATTGCTGCT GCTGCTGCTG CTGCTGCTGG GGGCTGGGGT GCCAGGTGCC 
TGGGGTCAGG CTGGGAGCCT GGACTTGCAG ATTGATGAGG AGCAGCCAGC GGGTACACTG 
ATTGGCGACA TCAGTGCGGG GCTTCCGGCA GGCACGGCAG CTCCTCTCAT GTACTTCATC 
TCTGCCCAAG AGGGCAGCGG CGTGGGCACA GACCTGGCCA TTGACGAACA CAGTGGGGTC 
GTCCGTACAG CCCGTGTCTT GGACCGTGAG CAGCGGGACC GCTACCGCTT CACTGCAGTC 
ACTCCTGATG GTGCCACCGT AGAAGTTACA GTGCGAGTGG CTGACATCAA CGACCATGCT 
CCAGCCTTCC CACAGGCTCG GGCTGCCCTG CAGGTACCTG AGCATACAGC TTTTGGCACC 
CGCTACCCAC TGGAGCCTGC TCGTGATGCA GATGCTGGGC GTCTGGGAAC CCAGGGCTAT 
GCGCTATCTG GTGATGGGGC TGGAGAGACC TTCCGGCTGG AGACACGCCC CGGTCCAGAT 
GGGACTCCAG TACCTGAGCT GGTAGTTACT GGGGAACTGG ACCGAGAGAA CCGCTCACAC 
TATATGCTAC AGCTGGAGGC CTATGATGGT GGTTCACCCC CCCGGAGGGC CCAGGCCCTG 
CTGGACGTGA CACTGCTGGA CATCAATGAC CATGCCCCGG CTTTCAATCA GAGCCGCTAC 
CATGCTGTGG TGTCTGAGAG CCTGGCCCCT GGCAGTCCTG TCTTGCAGGT GTTCGCATCT 
GATGCCGATG CTGGTGTCAA TGGGGCTGTG ACTTACGAGA TCAACCGGAG GCAGAGCGAG 
GGTGATGGAC CCTTCTCCAT CGACGCACAC ACGGGGCTGC TGCAGTTAGA GCGGCCACTG 
GACTTTGAGC AGCGGCGGGT CCATGAACTG GTGGTGCAAG CACGAGATGG TGGGGCTCAC 
CCTGAGCTGG GCTCGGCCTT TGTGACTGTG CATGTGCGAG ATGCCAATGA CAATCAGCCC 
TCCATGACTG TCATCTTTCT CAGTGCAGAT GGCTCCCCCC AAGTGTCTGA GGCCGCCCCA 
CCTGGACAGC TCGTTGCTCG CATCTCTGTG TCAGACCCAG ATGATGGTGA CTTTGCCCAT 
GTCAATGTGT CCCTGGAAGG TGGAGAGGGC CACTTTGCCC TAAGCACCCA AGACAGCGTC 
ATCTATCTGG TGTGTGTGGC TCGGCGGCTG GATCGAGAGG AGAGGGATGC CTATAACTTG 
AGGGTTACAG CCACAGACTC AGGCTCACCT CCACTGCGGG CTGAGGCTGC CTTTGTGCTG 
CACGTCACTG ATGTCAACGA CAATGCACCT GCCTTTGACC GCCAGCTCTA CCGACCTGAG 
CCCCTGCCTG AGGTTGCGCT GCCTGGCAGC TTTGTAGTGC GGGTGACTGC TCGGGATCCT 
GACCAAGGCA CCAATGGTCA GGTCACTTAT AGCCTAGCCC CTGGCGCCCA CACCCACTGG 
TTCTCCATTG ACCCCACCTC AGGCATTATC ACTACGGCTG CCTCACTGGA CTATGAGTTG 
GAACCTCAGC CACAGCTGAT TGTGGTGGCC ACAGATGGTG GCCTGCCCCC TCTAGCCTCC 
TCTGCCACAG TTAGCGTGGC CCTGCAAGAT GTGAATGATA ATGAGCCCCA ATTCCAGAGG 
ACTTTCTACA ATGCCTCACT GCCTGAGGGC ACCCAGCCTG GAACTTGCTT CCTGCAGGTG 
ACAGCCACAG ACGCGGATAG TGGCCCATTT GGCCTCCTCT CCTATTCCTT GGGTGCTGGA 
CTTGGGTCCT CCGGATCTCC CCCATTCCGC ATTGATGCCC ACAGCGGTGA TGTGTGCACA 
ACCCGGACCC TGGACCGTGA CCAGGGGCCC TCAAGCTTTG ACTTCACAGT GACAGCTGTG 
GATGGGGGAG GCCTCAAGTC CATGGTATAT GTGAAGGTGT TTCTGTCAGA CGAGAATGAC 
AACCCTCCTC AGTTTTATCC ACGGGAGTAT GCTGCCAGTA TAAGTGCCCA GAGTCCACCA 
GGCACAGCTG TGCTGAGGTT GCGTGCCCAT GACCCTGACC AGGGATCCCA TGGGCGACTC 
TCCTACCATA TCCTGGCTGG CAACAGCCCC CCACTTTTTA CCTTGGATGA GCAATCAGGG 
CTGTTGACAG TAGCCTGGCC CTTGGCCAGA CGGGCCAATT CTGTGGTGCA GCTGGAGATC 
GGGGCTGAGG ACGGAGGTGG CCTACAGGCA GAACCCAGTG CCCGAGTGGA CATCAGCATT 
GTGCCTGGAA CCCCCACACC ACCCATATTT GAGCAACTAC AGTATGTTTT TTCTGTGCCA 
GAGGATGTGG CACCAGGCAC CAGTGTGGGC ATAGTCCAGG CACACAACCC ACCAGGTCGC 
TTGGCACCTG TGACCCTTTC CCTATCAGGT GGGGATCCCC GAGGACTCTT CTCCCTAGAT 
GCGGTATCAG GACTGTTGCA AACACTTCGC CCTCTGGACC GGGAGCTACT GGGACCAGTG 
TTGGAGCTGG AGGTGCGAGC AGGCAGTGGA GTGCCCCCAG CTTTCGCTGT AGCTCGGGTG 
CGTGTGCTGC TGGATGATGT GAATGACAAC TCCCCTGCCT TTCCTGCACC TGAAGACACG 
GTATTGCTAC CACCAAACAC TGCCCCAGGG ACTCCCATCT ATACACTGCG GGCTCTTGAC 
CCCGACTCAG GTGTTAACAG TCGAGTCACC TTTACCCTGC TTGCTGGGGG TGGTGGAGCC 
TTCACCGTGG ACCCCACCAC AGGCCATGTA CGGCTTATGA GGCCTCTGGG GCCCTCAGGA 
GGGCCAGCCC ATGAGCTGGA GCTGGAGGCC CGGGATGGGG GCTCCCCACC ACGCACCAGC 
CACTTTCGAC TACGGGTGGT GGTACAGGAT GTGGGAACCC GTGGGCTGGC TCCCCGATTC 
AACAGCCCTA CCTACCGTGT GGACCTGCCC TCAGGCACCA CTGCTGGAAC TCAGGTCCTG 
CAAGTGCAGG CCCAAGCACC AGATGGGGGC CCTATCACCT ATCACCTTGC AGCAGAGGGA 
GCAAGTAGCC CCTTTGGCCT GGAGCCACAG AGTGGGTGGC TATGGGTGCG GGCAGCACTA 
GACCGTGAGG CCCAGGAATT GTACATACTG AAGGTAATGG CAGTGTCTGG GTCCAAAGCT 
GAGTTGGGGC AGCAGACAGG CACAGCCACC GTGAGGGTCA GCATCCTCAA CCAGAATGAA 
CACAGTCCCC GCTTGTCTGA GGATCCCACC TTCCTGGCTG TGGCTGAGAA CCAGCCCCCA 
GGGACCAGCG TGGGCCGAGT CTTTGCCACT GACCGAGACT CAGGACCCAA TGGACGTCTG 
ACCTACAGCC TGCAACAGCT GTCTGAAGAC AGCAAGGCCT TCCGCATCCA CCCCCAGACT 
GGAGAAGTGA CCACACTCCA AACCCTGGAC CGTGAGCAGC AGAGCAGCTA TCAGCTCCTG 
GTGCAGGTGC AGGATGGAGG GAGCCCACCC CGCAGCACCA CAGGCACTGT GCATGTTGCA 
GTGCTTGACC TCAACGACAA CAGCCCCACG TTCCTGCAGG CTTCAGGAGC TGCTGGTGGG 
GGCCTCCCTA TACAGGTACC AGACCGCGTG CCTCCGGGAA CACTGGTGAC GACTCTGCAG 
GCGAAGGATC CAGATGAGGG GGAGAATGGG ACCATCTTGT ACACGCTAAC TGGTCCTGGC 
TCAGAGCTTT TCTCTCTGCA CCCTCACTCA GGGGAGCTGC TCACTGCAGC TCCCCTGATC 
CGAGCAGAGC GGCCCCACTA TGTGCTGACA CTGAGTGCTC ATGACCAAGG CAGCCCTCCT 
CGAAGTGCCA GCCTCCAGCT GCTGGTGCAG GTGCTTCCCT CAGCTCGCTT GGCCGAGCCG 
CCCCCAGATC TCGCAGAGCG GGACCCAGCG GCACCAGTGC CTGTCGTGCT GACGGTGACA 
GCAGCTGAGG GACTGCGGCC CGGCTCTCTG TTGGGCTCGG TGGCAGCGCC AGAGCCCGCG 
GGTGTGGGTG CACTCACCTA CACACTGGTG GGCGGTGCCG ATCCCGAGGG CACCTTCGCG 
CTGGATGCGG CCTCAGGGCG CTTGTACCTG GCGCGGCCCC TGGACTTCGA AGCTGGCCCG 
CCGTGGCGCG CGCTTACGGT ACGCGCTGAG GGGCCGGGAG GCGCGGGCGC GCGGCTGCTG 
CGAGTGCAGG TGCAAGTGCA GGACGAGAAT GAGCATGCGC CCGCCTTTGC GCGCGACCCG 
CTGGCGCTGG CGCTGCCAGA GAACCCGGAG CCCGGCGCAG CGCTGTACAC TTTCCGCGCG 
TCGGACGCCG ACGGCCCCGG CCCCAATAGC GACGTGCGCT ACCGCCTGCT GCGCCAGGAG 
CCGCCCGTGC CGGCGCTTCG CCTGGACGCG CGCACCGGGG CGCTCAGCGC TCCGCGCGGC 
CTGGACCGAG AGACCACTCC CGCGCTGCTG CTGCTGGTGG AAGCCACCGA CCGGCCCGCC 
AACGCCAGCC GCCGTCGTGC AGCGCGCGTT TCAGCGCGCG TCTTCGTCAC GGATGAGAAT 
GACAACGCGC CTGTCTTCGC CTCGCCGTCA CGCGTGCGCC TCCCAGAGGA CCAGCCGCCT 
GGGCCCGCGG CCCTGCACGT GGTAGCCCGG GACCCGGATC TGGGCGAGGC TGCACGCGTG 
TCCTATCGGC TGGCATCTGG CGGGGACGGC CACTTCCGGC TGCACTCAAG CACTGGAGCG 
CTGTCCGTGG TGCGGCCGTT GGACCGCGAA CAACGAGCTG AGCACGTACT GACAGTGGTG 
GCCTCAGACC ACGGCTCCCC GCCGCGCTCG GCCACGCAGG TCCTGACCGT CAGTGTCGCT 
GACGTCAACG ACGAGGCGCC TACTTTCCAG CAGCAGGAGT ACAGCGTCCT CTTGCGTGAG 
AACAACCCTC CTGGCACATC TCTGCTCACC CTGCGAGCAA CCGACCCCGA CGTGGGGGCC 
AACGGGCAAG TGACTTATGG AGGCGTCTCT AGCGAAAGCT TTTCTCTGGA TCCTGACACT 
GGTGTTCTCA CGACTCTTCG GGCCCTGGAT CGAGAGGAAC AGGAGGAGAT CAACCTGACA 
GTGTATGCCC AGGACAGGGG CTCACCTCCT CAGTTAACGC ATGTCACTGT TCGAGTGGCT 
GTGGAGGATG AGAATGACCA TGCACCAACC TTTGGGAGTG CCCATCTCTC TCTGGAGGTG 
CCTGAGGGCC AGGACCCCCA GACCCTTACC ATGCTTCGGG CCTCTGATCC AGATGTGGGA 
GCCAATGGGC AGTTGCAGTA CCGCATCCTA GATGGGGACC CATCAGGAGC CTTTGTCCTA 
GACCTTGCTT CTGGAGAGTT TGGCACCATG CGGCCACTAG ACAGAGAAGT GGAGCCAGCT 
TTCCAGCTGA GGATAGAGGC CCGGGATGGA GGCCAGCCAG CTCTCAGTGC CACGCTGCTT 
TTGACAGTGA CAGTGCTGGA TGCCAATGAC CATGCTCCAG CCTTTCCTGT GCCTGCCTAC 
TCGGTGGAGG TGCCGGAGGA TGTGCCTGCA GGGACCCTGC TGCTGCAGCT ACAGGCTCAT 
GACCCTGATG CTGGAGCTAA TGGCCATGTG ACCTACTACC TGGGCGCCGG TACAGCAGGA 
GCCTTCCTGC TGGAGCCCAG CTCTGGAGAA CTGCGCACAG CTGCAGCCTT GGACAGAGAA 
CAGTGTCCCA GCTACACCTT TTCTGTGAGT GCAGTGGATG GTGCAGCTGC TGGGCCCCTA 
AGCACCACAG TGTCTGTCAC CATCACGGTG CGCGATGTCA ATGACCATGC ACCCACCTTC 
CCCACCAGTC CTCTGCGCCT ACGTCTGCCC CGCCCAGGCC CCAGCTTCAG TACCCCAACC 
CTGGCTCTGG CCACACTGAG AGCTGAAGAT CGTGATGCTG GTGCCAATGC TTCCATTCTG 
TACCGGCTGG CAGGCACACC ACCTCCTGGC ACTACTGTGG ACTCTTACAC TGGTGAAATC 
CGCGTGGCCC GCTCTCCTGT AGCTCTAGGC CCCCGAGATC GTGTCCTCTT CATTGTGGCC 
ACTGATCTTG GCCGTCCAGC TCGCTCTGCC ACTGGTGTGA TCATTGTTGG ACTGCAGGGG 
GAAGCTGAGC GTGGACCCCG CTTTCCCCGG GCTAGCAGTG AGGCTACGAT TCGTGAGAAT 
GCGCCCCCAG GGACTCCTAT TGTCTCCCCC AGGGCCGTCC ATGCAGGAGG CACAAATGGA 
CCCATCACCT ACAGCATTCT CAGTGGGAAT GAGAAAGGGA CATTCTCCAT CCAGCCTAGT 
ACAGGTGCCA TCACAGTTCG CTCAGCAGAG GGGCTAGACT TCGAGGTGAG TCCACGGCTG 
CGACTGGTGC TGCAGGCAGA GAGTGGAGGA GCCTTTGCCT TCACTGTGCT GACCCTGACC 
CTGCAAGATG CCAACGACAA TGCTCCCCGT TTCCTGCGGC CCCATTATGT GGCCTTCCTT 
CCTGAGTCCC GGCCCTTGGA GGGGCCCCTG CTGCAGGTGG AGGCGGATGA CCTGGATCAA 
GGCTCTGGAG GACAGATTTC CTACAGTCTG GCTGCATCCC AGCCGGCACG TGGATTGTTC 
CACGTAGACC CAACCACAGG CACTATCACT ACCACAGCCA TCCTGGACCG TGAGATCTGG 
GCTGAAACAC GGTTGGTGCT GATGGCCACA GACAGAGGGA GCCCAGCCCT GGTGGGCTCA 
GCTACCTTGA CGGTGATGGT CATCGACACC AATGACAATC GCCCCACCAT CCCCCAACCC 
TGGGAGCTCC GAGTGTCAGA AGATGCGTTA TTGGGCTCAG AGATTGCACA GGTAACAGGG 
AATGATGTGG ACTCAGGACC CGTGCTGTGG TATGTGCTAA GCCCATCTGG GCCCCAGGAT 
CCCTTCAGTG TTGGCCGCTA TGGAGGCCGT GTCTCCCTCA CGGGGCCCCT GGACTTTGAG 
CAGTGTGACC GCTACCAGCT GCAGCTGCTG GCACATGATG GGCCTCATGA GGGCCGTGCC 
AACCTCACAG TGCTTGTGGA GGATGTCAAT GACAATGCAC CTGCCTTCTC ACAGAGCCTC 
TACCAGGTAA TGCTGCTTGA GCACACACCC CCAGGCAGTG CCATTCTCTC CGTCTCTGCC 
ACTGATCGGG ACTCAGGTGC CAACGGTCAC ATTTCCTACC ACCTGGCTTC CCCTGCCGAT 
GGCTTCAGTG TTGACCCCAA CAATGGGACC CTGTTCACAA TAGTGGGAAC AGTGGCCTTG 
GGCCATGACG GGTCAGGAGC AGTGGATGTG GTGCTGGAAG CACGAGACCA CGGGGCTCCA 
GGCCGGGCAG CACGAGCCAC AGTGCACGTG CAGCTGCAGG ACCAGAACGA CCACGCCCCG 
AGCTTCACAT TGTCACACTA CCGTGTGGCT GTGACTGAAG ACCTGCCCCC TGGCTCCACT 
CTGCTCACCC TGGAGGCTAC AGATGCTGAT GGAAGCCGCA GCCATGCCGC TGTGGACTAC 
AGCATCATCA GTGGCAACTG GGGCCGAGTC TTCCAGCTGG AACCCAGGCT GGCTGAGGCT 
GGGGAGAGTG CTGGACCAGG CCCCCGGGCA CTGGGCTGCC TGGTGTTGCT TGAACCTCTA 
GACTTTGAAA GCCTGACACA GTACAATCTA ACAGTGGCTG CAGCTGACCG TGGGCAGCCA 
CCCCAAAGCT CAGTCGTGCC AGTCACTGTC ACTGTACTAG ATGTCAATGA CAACCCACCT 
GTCTTTACCC GAGCATCCTA CCGTGTGACA GTACCTGAGG ACACACCTGT TGGAGCTGAG 
CTGCTGCATG TAGAGGCCTC TGACGCTGAC CCTGGCCCTC ATGGCCTCGT GCGTTTCACT 
GTCAGCTCAG GCGACCCATC AGGGCTCTTT GAGCTGGATG AGAGCTCAGG CACCTTGCGA 
CTGGCCCATG CCCTGGACTG TGAGACCCAG GCTCGACATC AGCTTGTAGT ACAGGCTGCT 
GACCCTGCTG GTGCACACTT TGCTTTGGCA CCAGTGACAA TTGAGGTCCA GGATGTGAAT 
GATCATGGCC CAGCCTTCCC ACTGAACTTA CTCAGCACCA GCGTGGCCGA GAATCAGCCT 
CCAGGCACTC TCGTGACCAC TCTGCATGCA ATCGACGGGG ATGCTGGGGC TTTTGGGAGG 
CTCCGTTACA GCCTGTTGGA GGCTGGGCCA GGACCTGAGG GCCGTGAGGC ATTTGCACTG 
AACAGCTCAA CAGGGGAGTT GCGTGCGCGA GTGCCCTTTG ACTATGAGCA CACAGAAAGC 
TTCCGGCTGC TGGTGGGTGC TGCTGATGCT GGGAATCTCT CAGCCTCTGT CACTGTGTCG 
GTGCTAGTGA CTGGAGAGGA TGAGTATGAC CCTGTATTTC TGGCACCAGC TTTCCACTTC 
CAAGTGCCCG AAGGTGCCCG GCGTGGCCAC AGCTTGGGTC ACGTGCAGGC CACAGATGAG 
GATGGGGGTG CCGATGGCCT GGTTCTGTAT TCCCTTGCCA CCTCTTCCCC CTATTTTGGT 
ATTAACCAGA CTACAGGAGC CCTGTACCTG CGGGTGGACA GTCGGGCACC AGGCAGCGGA 
ACAGCCACCT CTGGGGGTGG GGGCCGGACC CGGCGGGAAG CACCACGGGA GCTGAGGCTG 
GAGGTGATAG CACGGGGGCC TCTGCCTGGT TCCCGGAGTG CCACAGTGCC TGTGACCGTG 
GATATCACCC ACACCGCACT GGGCCTGGCA CCTGACCTCA ACCTGCTATT AGTAGGGGCC 
GTGGCAGCCT CCTTGGGAGT TGTGGTGGTG CTTGCACTGG CAGCCCTGGT CCTAGGACTT 
GTTCGGGCCC GTAGCCGCAA GGCTGAGGCA GCCCCTGGCC CAATGTCACA GGCAGCACCC 
CTAGCCAGTG ACTCACTGCA GAAACTGGGC CGGGAGCCAC CTAGTCCACC ACCCTCTGAG 
CACCTCTATC ACCAGACTCT TCCCAGCTAT GGTGGGCCAG GAGCTGGAGG ACCCTACCCC 
CGTGGTGGCT CCTTGGACCC TTCACATTCA AGTGGCCGAG GATCAGCAGA GGCTGCAGAG 
GATGATGAGA TCCGCATGAT CAATGAGTTC CCCCGTGTGG CCAGTGTGGC CTCCTCTCTG 
GCTGCCCGTG GCCCTGACTC AGGCATCCAG CAGGATGCAG ATGGTCTGAG TGACACATCC 
TGCGAACCAC CTGCCCCTGA CACCTGGTAT AAGGGCCGAA AGGCAGGGCT GCTGCTGCCA 
GGTGCAGGAG CCACTCTCTA CAGAGAGGAG GGGCCCCCAG CCACTGCCAC AGCCTTCCTG 
GGGGGCTGTG GCCTGAGCCC TGCACCCACT GGGGACTATG GCTTCCCAGC AGATGGCAAG 
CCATGTGTGG CAGGTGCGCT GACAGCCATT GTGGCCGGCG AGGAGGAGCT CCGTGGCAGC 
TATAACTGGG ACTACCTGCT GAGCTGGTGC CCTCAGTTCC AACCACTGGC CAGTGTCTTC 
ACAGAGATCG CTCGGCTCAA GGATGAAGCT CGGCCATGTC CCCCAGCTCC CCGTATCGAC 
CCACCACCCC TCATCACTGC CGTGGCCCAC CCAGGAGCCA AGTCTGTGCC CCCCAAGCCA 
GCAAACACAG CTGCAGCCCG GGCCATCTTC CCACCAGCTT CTCACCGCTC CCCCATCAGC 
CATGAAGGCT CCCTGTCCTC AGCTGCCATG TCCCCCAGCT TCTCACCCTC TCTGTCTCCT 
CTGGCTGCTC GCTCACCCGT TGTCTCACCA TTTGGGGTGG CCCAGGGTCC CTCAGCCTCA 
GCACTCAGCG CAGAGTCTGG CCTGGAGCCA CCTGATGACA CGGAGCTGCA CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCHS1 ( NM_003737.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003728.1 Homo sapiens dachsous cadherin-related 1 (DCHS1), mRNA.

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CloneID	OHu21465
Clone ID Related Accession (Same CDS sequence)	NM_003737.3 , NM_003737.4
Accession Version	NM_003737.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	protocadherin-16 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB053446.1 and AC091564.12. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_003737.3. ##Evidence-Data-START## Transcript exon combination :: AB053446.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000299441.5/ ENSP00000299441.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGAAGG AGCTGGGCAT TGTGCCTTCC TGCCCTGGCA TGAAGAGCCC CAGGCCCCAC 
CTCCTGCTAC CATTGCTGCT GCTGCTGCTG CTGCTGCTGG GGGCTGGGGT GCCAGGTGCC 
TGGGGTCAGG CTGGGAGCCT GGACTTGCAG ATTGATGAGG AGCAGCCAGC GGGTACACTG 
ATTGGCGACA TCAGTGCGGG GCTTCCGGCA GGCACGGCAG CTCCTCTCAT GTACTTCATC 
TCTGCCCAAG AGGGCAGCGG CGTGGGCACA GACCTGGCCA TTGACGAACA CAGTGGGGTC 
GTCCGTACAG CCCGTGTCTT GGACCGTGAG CAGCGGGACC GCTACCGCTT CACTGCAGTC 
ACTCCTGATG GTGCCACCGT AGAAGTTACA GTGCGAGTGG CTGACATCAA CGACCATGCT 
CCAGCCTTCC CACAGGCTCG GGCTGCCCTG CAGGTACCTG AGCATACAGC TTTTGGCACC 
CGCTACCCAC TGGAGCCTGC TCGTGATGCA GATGCTGGGC GTCTGGGAAC CCAGGGCTAT 
GCGCTATCTG GTGATGGGGC TGGAGAGACC TTCCGGCTGG AGACACGCCC CGGTCCAGAT 
GGGACTCCAG TACCTGAGCT GGTAGTTACT GGGGAACTGG ACCGAGAGAA CCGCTCACAC 
TATATGCTAC AGCTGGAGGC CTATGATGGT GGTTCACCCC CCCGGAGGGC CCAGGCCCTG 
CTGGACGTGA CACTGCTGGA CATCAATGAC CATGCCCCGG CTTTCAATCA GAGCCGCTAC 
CATGCTGTGG TGTCTGAGAG CCTGGCCCCT GGCAGTCCTG TCTTGCAGGT GTTCGCATCT 
GATGCCGATG CTGGTGTCAA TGGGGCTGTG ACTTACGAGA TCAACCGGAG GCAGAGCGAG 
GGTGATGGAC CCTTCTCCAT CGACGCACAC ACGGGGCTGC TGCAGTTAGA GCGGCCACTG 
GACTTTGAGC AGCGGCGGGT CCATGAACTG GTGGTGCAAG CACGAGATGG TGGGGCTCAC 
CCTGAGCTGG GCTCGGCCTT TGTGACTGTG CATGTGCGAG ATGCCAATGA CAATCAGCCC 
TCCATGACTG TCATCTTTCT CAGTGCAGAT GGCTCCCCCC AAGTGTCTGA GGCCGCCCCA 
CCTGGACAGC TCGTTGCTCG CATCTCTGTG TCAGACCCAG ATGATGGTGA CTTTGCCCAT 
GTCAATGTGT CCCTGGAAGG TGGAGAGGGC CACTTTGCCC TAAGCACCCA AGACAGCGTC 
ATCTATCTGG TGTGTGTGGC TCGGCGGCTG GATCGAGAGG AGAGGGATGC CTATAACTTG 
AGGGTTACAG CCACAGACTC AGGCTCACCT CCACTGCGGG CTGAGGCTGC CTTTGTGCTG 
CACGTCACTG ATGTCAACGA CAATGCACCT GCCTTTGACC GCCAGCTCTA CCGACCTGAG 
CCCCTGCCTG AGGTTGCGCT GCCTGGCAGC TTTGTAGTGC GGGTGACTGC TCGGGATCCT 
GACCAAGGCA CCAATGGTCA GGTCACTTAT AGCCTAGCCC CTGGCGCCCA CACCCACTGG 
TTCTCCATTG ACCCCACCTC AGGCATTATC ACTACGGCTG CCTCACTGGA CTATGAGTTG 
GAACCTCAGC CACAGCTGAT TGTGGTGGCC ACAGATGGTG GCCTGCCCCC TCTAGCCTCC 
TCTGCCACAG TTAGCGTGGC CCTGCAAGAT GTGAATGATA ATGAGCCCCA ATTCCAGAGG 
ACTTTCTACA ATGCCTCACT GCCTGAGGGC ACCCAGCCTG GAACTTGCTT CCTGCAGGTG 
ACAGCCACAG ACGCGGATAG TGGCCCATTT GGCCTCCTCT CCTATTCCTT GGGTGCTGGA 
CTTGGGTCCT CCGGATCTCC CCCATTCCGC ATTGATGCCC ACAGCGGTGA TGTGTGCACA 
ACCCGGACCC TGGACCGTGA CCAGGGGCCC TCAAGCTTTG ACTTCACAGT GACAGCTGTG 
GATGGGGGAG GCCTCAAGTC CATGGTATAT GTGAAGGTGT TTCTGTCAGA CGAGAATGAC 
AACCCTCCTC AGTTTTATCC ACGGGAGTAT GCTGCCAGTA TAAGTGCCCA GAGTCCACCA 
GGCACAGCTG TGCTGAGGTT GCGTGCCCAT GACCCTGACC AGGGATCCCA TGGGCGACTC 
TCCTACCATA TCCTGGCTGG CAACAGCCCC CCACTTTTTA CCTTGGATGA GCAATCAGGG 
CTGTTGACAG TAGCCTGGCC CTTGGCCAGA CGGGCCAATT CTGTGGTGCA GCTGGAGATC 
GGGGCTGAGG ACGGAGGTGG CCTACAGGCA GAACCCAGTG CCCGAGTGGA CATCAGCATT 
GTGCCTGGAA CCCCCACACC ACCCATATTT GAGCAACTAC AGTATGTTTT TTCTGTGCCA 
GAGGATGTGG CACCAGGCAC CAGTGTGGGC ATAGTCCAGG CACACAACCC ACCAGGTCGC 
TTGGCACCTG TGACCCTTTC CCTATCAGGT GGGGATCCCC GAGGACTCTT CTCCCTAGAT 
GCGGTATCAG GACTGTTGCA AACACTTCGC CCTCTGGACC GGGAGCTACT GGGACCAGTG 
TTGGAGCTGG AGGTGCGAGC AGGCAGTGGA GTGCCCCCAG CTTTCGCTGT AGCTCGGGTG 
CGTGTGCTGC TGGATGATGT GAATGACAAC TCCCCTGCCT TTCCTGCACC TGAAGACACG 
GTATTGCTAC CACCAAACAC TGCCCCAGGG ACTCCCATCT ATACACTGCG GGCTCTTGAC 
CCCGACTCAG GTGTTAACAG TCGAGTCACC TTTACCCTGC TTGCTGGGGG TGGTGGAGCC 
TTCACCGTGG ACCCCACCAC AGGCCATGTA CGGCTTATGA GGCCTCTGGG GCCCTCAGGA 
GGGCCAGCCC ATGAGCTGGA GCTGGAGGCC CGGGATGGGG GCTCCCCACC ACGCACCAGC 
CACTTTCGAC TACGGGTGGT GGTACAGGAT GTGGGAACCC GTGGGCTGGC TCCCCGATTC 
AACAGCCCTA CCTACCGTGT GGACCTGCCC TCAGGCACCA CTGCTGGAAC TCAGGTCCTG 
CAAGTGCAGG CCCAAGCACC AGATGGGGGC CCTATCACCT ATCACCTTGC AGCAGAGGGA 
GCAAGTAGCC CCTTTGGCCT GGAGCCACAG AGTGGGTGGC TATGGGTGCG GGCAGCACTA 
GACCGTGAGG CCCAGGAATT GTACATACTG AAGGTAATGG CAGTGTCTGG GTCCAAAGCT 
GAGTTGGGGC AGCAGACAGG CACAGCCACC GTGAGGGTCA GCATCCTCAA CCAGAATGAA 
CACAGTCCCC GCTTGTCTGA GGATCCCACC TTCCTGGCTG TGGCTGAGAA CCAGCCCCCA 
GGGACCAGCG TGGGCCGAGT CTTTGCCACT GACCGAGACT CAGGACCCAA TGGACGTCTG 
ACCTACAGCC TGCAACAGCT GTCTGAAGAC AGCAAGGCCT TCCGCATCCA CCCCCAGACT 
GGAGAAGTGA CCACACTCCA AACCCTGGAC CGTGAGCAGC AGAGCAGCTA TCAGCTCCTG 
GTGCAGGTGC AGGATGGAGG GAGCCCACCC CGCAGCACCA CAGGCACTGT GCATGTTGCA 
GTGCTTGACC TCAACGACAA CAGCCCCACG TTCCTGCAGG CTTCAGGAGC TGCTGGTGGG 
GGCCTCCCTA TACAGGTACC AGACCGCGTG CCTCCGGGAA CACTGGTGAC GACTCTGCAG 
GCGAAGGATC CAGATGAGGG GGAGAATGGG ACCATCTTGT ACACGCTAAC TGGTCCTGGC 
TCAGAGCTTT TCTCTCTGCA CCCTCACTCA GGGGAGCTGC TCACTGCAGC TCCCCTGATC 
CGAGCAGAGC GGCCCCACTA TGTGCTGACA CTGAGTGCTC ATGACCAAGG CAGCCCTCCT 
CGAAGTGCCA GCCTCCAGCT GCTGGTGCAG GTGCTTCCCT CAGCTCGCTT GGCCGAGCCG 
CCCCCAGATC TCGCAGAGCG GGACCCAGCG GCACCAGTGC CTGTCGTGCT GACGGTGACA 
GCAGCTGAGG GACTGCGGCC CGGCTCTCTG TTGGGCTCGG TGGCAGCGCC AGAGCCCGCG 
GGTGTGGGTG CACTCACCTA CACACTGGTG GGCGGTGCCG ATCCCGAGGG CACCTTCGCG 
CTGGATGCGG CCTCAGGGCG CTTGTACCTG GCGCGGCCCC TGGACTTCGA AGCTGGCCCG 
CCGTGGCGCG CGCTTACGGT ACGCGCTGAG GGGCCGGGAG GCGCGGGCGC GCGGCTGCTG 
CGAGTGCAGG TGCAAGTGCA GGACGAGAAT GAGCATGCGC CCGCCTTTGC GCGCGACCCG 
CTGGCGCTGG CGCTGCCAGA GAACCCGGAG CCCGGCGCAG CGCTGTACAC TTTCCGCGCG 
TCGGACGCCG ACGGCCCCGG CCCCAATAGC GACGTGCGCT ACCGCCTGCT GCGCCAGGAG 
CCGCCCGTGC CGGCGCTTCG CCTGGACGCG CGCACCGGGG CGCTCAGCGC TCCGCGCGGC 
CTGGACCGAG AGACCACTCC CGCGCTGCTG CTGCTGGTGG AAGCCACCGA CCGGCCCGCC 
AACGCCAGCC GCCGTCGTGC AGCGCGCGTT TCAGCGCGCG TCTTCGTCAC GGATGAGAAT 
GACAACGCGC CTGTCTTCGC CTCGCCGTCA CGCGTGCGCC TCCCAGAGGA CCAGCCGCCT 
GGGCCCGCGG CCCTGCACGT GGTAGCCCGG GACCCGGATC TGGGCGAGGC TGCACGCGTG 
TCCTATCGGC TGGCATCTGG CGGGGACGGC CACTTCCGGC TGCACTCAAG CACTGGAGCG 
CTGTCCGTGG TGCGGCCGTT GGACCGCGAA CAACGAGCTG AGCACGTACT GACAGTGGTG 
GCCTCAGACC ACGGCTCCCC GCCGCGCTCG GCCACGCAGG TCCTGACCGT CAGTGTCGCT 
GACGTCAACG ACGAGGCGCC TACTTTCCAG CAGCAGGAGT ACAGCGTCCT CTTGCGTGAG 
AACAACCCTC CTGGCACATC TCTGCTCACC CTGCGAGCAA CCGACCCCGA CGTGGGGGCC 
AACGGGCAAG TGACTTATGG AGGCGTCTCT AGCGAAAGCT TTTCTCTGGA TCCTGACACT 
GGTGTTCTCA CGACTCTTCG GGCCCTGGAT CGAGAGGAAC AGGAGGAGAT CAACCTGACA 
GTGTATGCCC AGGACAGGGG CTCACCTCCT CAGTTAACGC ATGTCACTGT TCGAGTGGCT 
GTGGAGGATG AGAATGACCA TGCACCAACC TTTGGGAGTG CCCATCTCTC TCTGGAGGTG 
CCTGAGGGCC AGGACCCCCA GACCCTTACC ATGCTTCGGG CCTCTGATCC AGATGTGGGA 
GCCAATGGGC AGTTGCAGTA CCGCATCCTA GATGGGGACC CATCAGGAGC CTTTGTCCTA 
GACCTTGCTT CTGGAGAGTT TGGCACCATG CGGCCACTAG ACAGAGAAGT GGAGCCAGCT 
TTCCAGCTGA GGATAGAGGC CCGGGATGGA GGCCAGCCAG CTCTCAGTGC CACGCTGCTT 
TTGACAGTGA CAGTGCTGGA TGCCAATGAC CATGCTCCAG CCTTTCCTGT GCCTGCCTAC 
TCGGTGGAGG TGCCGGAGGA TGTGCCTGCA GGGACCCTGC TGCTGCAGCT ACAGGCTCAT 
GACCCTGATG CTGGAGCTAA TGGCCATGTG ACCTACTACC TGGGCGCCGG TACAGCAGGA 
GCCTTCCTGC TGGAGCCCAG CTCTGGAGAA CTGCGCACAG CTGCAGCCTT GGACAGAGAA 
CAGTGTCCCA GCTACACCTT TTCTGTGAGT GCAGTGGATG GTGCAGCTGC TGGGCCCCTA 
AGCACCACAG TGTCTGTCAC CATCACGGTG CGCGATGTCA ATGACCATGC ACCCACCTTC 
CCCACCAGTC CTCTGCGCCT ACGTCTGCCC CGCCCAGGCC CCAGCTTCAG TACCCCAACC 
CTGGCTCTGG CCACACTGAG AGCTGAAGAT CGTGATGCTG GTGCCAATGC TTCCATTCTG 
TACCGGCTGG CAGGCACACC ACCTCCTGGC ACTACTGTGG ACTCTTACAC TGGTGAAATC 
CGCGTGGCCC GCTCTCCTGT AGCTCTAGGC CCCCGAGATC GTGTCCTCTT CATTGTGGCC 
ACTGATCTTG GCCGTCCAGC TCGCTCTGCC ACTGGTGTGA TCATTGTTGG ACTGCAGGGG 
GAAGCTGAGC GTGGACCCCG CTTTCCCCGG GCTAGCAGTG AGGCTACGAT TCGTGAGAAT 
GCGCCCCCAG GGACTCCTAT TGTCTCCCCC AGGGCCGTCC ATGCAGGAGG CACAAATGGA 
CCCATCACCT ACAGCATTCT CAGTGGGAAT GAGAAAGGGA CATTCTCCAT CCAGCCTAGT 
ACAGGTGCCA TCACAGTTCG CTCAGCAGAG GGGCTAGACT TCGAGGTGAG TCCACGGCTG 
CGACTGGTGC TGCAGGCAGA GAGTGGAGGA GCCTTTGCCT TCACTGTGCT GACCCTGACC 
CTGCAAGATG CCAACGACAA TGCTCCCCGT TTCCTGCGGC CCCATTATGT GGCCTTCCTT 
CCTGAGTCCC GGCCCTTGGA GGGGCCCCTG CTGCAGGTGG AGGCGGATGA CCTGGATCAA 
GGCTCTGGAG GACAGATTTC CTACAGTCTG GCTGCATCCC AGCCGGCACG TGGATTGTTC 
CACGTAGACC CAACCACAGG CACTATCACT ACCACAGCCA TCCTGGACCG TGAGATCTGG 
GCTGAAACAC GGTTGGTGCT GATGGCCACA GACAGAGGGA GCCCAGCCCT GGTGGGCTCA 
GCTACCTTGA CGGTGATGGT CATCGACACC AATGACAATC GCCCCACCAT CCCCCAACCC 
TGGGAGCTCC GAGTGTCAGA AGATGCGTTA TTGGGCTCAG AGATTGCACA GGTAACAGGG 
AATGATGTGG ACTCAGGACC CGTGCTGTGG TATGTGCTAA GCCCATCTGG GCCCCAGGAT 
CCCTTCAGTG TTGGCCGCTA TGGAGGCCGT GTCTCCCTCA CGGGGCCCCT GGACTTTGAG 
CAGTGTGACC GCTACCAGCT GCAGCTGCTG GCACATGATG GGCCTCATGA GGGCCGTGCC 
AACCTCACAG TGCTTGTGGA GGATGTCAAT GACAATGCAC CTGCCTTCTC ACAGAGCCTC 
TACCAGGTAA TGCTGCTTGA GCACACACCC CCAGGCAGTG CCATTCTCTC CGTCTCTGCC 
ACTGATCGGG ACTCAGGTGC CAACGGTCAC ATTTCCTACC ACCTGGCTTC CCCTGCCGAT 
GGCTTCAGTG TTGACCCCAA CAATGGGACC CTGTTCACAA TAGTGGGAAC AGTGGCCTTG 
GGCCATGACG GGTCAGGAGC AGTGGATGTG GTGCTGGAAG CACGAGACCA CGGGGCTCCA 
GGCCGGGCAG CACGAGCCAC AGTGCACGTG CAGCTGCAGG ACCAGAACGA CCACGCCCCG 
AGCTTCACAT TGTCACACTA CCGTGTGGCT GTGACTGAAG ACCTGCCCCC TGGCTCCACT 
CTGCTCACCC TGGAGGCTAC AGATGCTGAT GGAAGCCGCA GCCATGCCGC TGTGGACTAC 
AGCATCATCA GTGGCAACTG GGGCCGAGTC TTCCAGCTGG AACCCAGGCT GGCTGAGGCT 
GGGGAGAGTG CTGGACCAGG CCCCCGGGCA CTGGGCTGCC TGGTGTTGCT TGAACCTCTA 
GACTTTGAAA GCCTGACACA GTACAATCTA ACAGTGGCTG CAGCTGACCG TGGGCAGCCA 
CCCCAAAGCT CAGTCGTGCC AGTCACTGTC ACTGTACTAG ATGTCAATGA CAACCCACCT 
GTCTTTACCC GAGCATCCTA CCGTGTGACA GTACCTGAGG ACACACCTGT TGGAGCTGAG 
CTGCTGCATG TAGAGGCCTC TGACGCTGAC CCTGGCCCTC ATGGCCTCGT GCGTTTCACT 
GTCAGCTCAG GCGACCCATC AGGGCTCTTT GAGCTGGATG AGAGCTCAGG CACCTTGCGA 
CTGGCCCATG CCCTGGACTG TGAGACCCAG GCTCGACATC AGCTTGTAGT ACAGGCTGCT 
GACCCTGCTG GTGCACACTT TGCTTTGGCA CCAGTGACAA TTGAGGTCCA GGATGTGAAT 
GATCATGGCC CAGCCTTCCC ACTGAACTTA CTCAGCACCA GCGTGGCCGA GAATCAGCCT 
CCAGGCACTC TCGTGACCAC TCTGCATGCA ATCGACGGGG ATGCTGGGGC TTTTGGGAGG 
CTCCGTTACA GCCTGTTGGA GGCTGGGCCA GGACCTGAGG GCCGTGAGGC ATTTGCACTG 
AACAGCTCAA CAGGGGAGTT GCGTGCGCGA GTGCCCTTTG ACTATGAGCA CACAGAAAGC 
TTCCGGCTGC TGGTGGGTGC TGCTGATGCT GGGAATCTCT CAGCCTCTGT CACTGTGTCG 
GTGCTAGTGA CTGGAGAGGA TGAGTATGAC CCTGTATTTC TGGCACCAGC TTTCCACTTC 
CAAGTGCCCG AAGGTGCCCG GCGTGGCCAC AGCTTGGGTC ACGTGCAGGC CACAGATGAG 
GATGGGGGTG CCGATGGCCT GGTTCTGTAT TCCCTTGCCA CCTCTTCCCC CTATTTTGGT 
ATTAACCAGA CTACAGGAGC CCTGTACCTG CGGGTGGACA GTCGGGCACC AGGCAGCGGA 
ACAGCCACCT CTGGGGGTGG GGGCCGGACC CGGCGGGAAG CACCACGGGA GCTGAGGCTG 
GAGGTGATAG CACGGGGGCC TCTGCCTGGT TCCCGGAGTG CCACAGTGCC TGTGACCGTG 
GATATCACCC ACACCGCACT GGGCCTGGCA CCTGACCTCA ACCTGCTATT AGTAGGGGCC 
GTGGCAGCCT CCTTGGGAGT TGTGGTGGTG CTTGCACTGG CAGCCCTGGT CCTAGGACTT 
GTTCGGGCCC GTAGCCGCAA GGCTGAGGCA GCCCCTGGCC CAATGTCACA GGCAGCACCC 
CTAGCCAGTG ACTCACTGCA GAAACTGGGC CGGGAGCCAC CTAGTCCACC ACCCTCTGAG 
CACCTCTATC ACCAGACTCT TCCCAGCTAT GGTGGGCCAG GAGCTGGAGG ACCCTACCCC 
CGTGGTGGCT CCTTGGACCC TTCACATTCA AGTGGCCGAG GATCAGCAGA GGCTGCAGAG 
GATGATGAGA TCCGCATGAT CAATGAGTTC CCCCGTGTGG CCAGTGTGGC CTCCTCTCTG 
GCTGCCCGTG GCCCTGACTC AGGCATCCAG CAGGATGCAG ATGGTCTGAG TGACACATCC 
TGCGAACCAC CTGCCCCTGA CACCTGGTAT AAGGGCCGAA AGGCAGGGCT GCTGCTGCCA 
GGTGCAGGAG CCACTCTCTA CAGAGAGGAG GGGCCCCCAG CCACTGCCAC AGCCTTCCTG 
GGGGGCTGTG GCCTGAGCCC TGCACCCACT GGGGACTATG GCTTCCCAGC AGATGGCAAG 
CCATGTGTGG CAGGTGCGCT GACAGCCATT GTGGCCGGCG AGGAGGAGCT CCGTGGCAGC 
TATAACTGGG ACTACCTGCT GAGCTGGTGC CCTCAGTTCC AACCACTGGC CAGTGTCTTC 
ACAGAGATCG CTCGGCTCAA GGATGAAGCT CGGCCATGTC CCCCAGCTCC CCGTATCGAC 
CCACCACCCC TCATCACTGC CGTGGCCCAC CCAGGAGCCA AGTCTGTGCC CCCCAAGCCA 
GCAAACACAG CTGCAGCCCG GGCCATCTTC CCACCAGCTT CTCACCGCTC CCCCATCAGC 
CATGAAGGCT CCCTGTCCTC AGCTGCCATG TCCCCCAGCT TCTCACCCTC TCTGTCTCCT 
CTGGCTGCTC GCTCACCCGT TGTCTCACCA TTTGGGGTGG CCCAGGGTCC CTCAGCCTCA 
GCACTCAGCG CAGAGTCTGG CCTGGAGCCA CCTGATGACA CGGAGCTGCA CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003728.1
CDS	368..10264
Translation	MQKELGIVPSCPGMKSPRPHLLLPLLLLLLLLLGAGVPGAWGQAGSLDLQIDEEQPAGTLIGDISAGLPAGTAAPLMYFISAQEGSGVGTDLAIDEHSGVVRTARVLDREQRDRYRFTAVTPDGATVEVTVRVADINDHAPAFPQARAALQVPEHTAFGTRYPLEPARDADAGRLGTQGYALSGDGAGETFRLETRPGPDGTPVPELVVTGELDRENRSHYMLQLEAYDGGSPPRRAQALLDVTLLDINDHAPAFNQSRYHAVVSESLAPGSPVLQVFASDADAGVNGAVTYEINRRQSEGDGPFSIDAHTGLLQLERPLDFEQRRVHELVVQARDGGAHPELGSAFVTVHVRDANDNQPSMTVIFLSADGSPQVSEAAPPGQLVARISVSDPDDGDFAHVNVSLEGGEGHFALSTQDSVIYLVCVARRLDREERDAYNLRVTATDSGSPPLRAEAAFVLHVTDVNDNAPAFDRQLYRPEPLPEVALPGSFVVRVTARDPDQGTNGQVTYSLAPGAHTHWFSIDPTSGIITTAASLDYELEPQPQLIVVATDGGLPPLASSATVSVALQDVNDNEPQFQRTFYNASLPEGTQPGTCFLQVTATDADSGPFGLLSYSLGAGLGSSGSPPFRIDAHSGDVCTTRTLDRDQGPSSFDFTVTAVDGGGLKSMVYVKVFLSDENDNPPQFYPREYAASISAQSPPGTAVLRLRAHDPDQGSHGRLSYHILAGNSPPLFTLDEQSGLLTVAWPLARRANSVVQLEIGAEDGGGLQAEPSARVDISIVPGTPTPPIFEQLQYVFSVPEDVAPGTSVGIVQAHNPPGRLAPVTLSLSGGDPRGLFSLDAVSGLLQTLRPLDRELLGPVLELEVRAGSGVPPAFAVARVRVLLDDVNDNSPAFPAPEDTVLLPPNTAPGTPIYTLRALDPDSGVNSRVTFTLLAGGGGAFTVDPTTGHVRLMRPLGPSGGPAHELELEARDGGSPPRTSHFRLRVVVQDVGTRGLAPRFNSPTYRVDLPSGTTAGTQVLQVQAQAPDGGPITYHLAAEGASSPFGLEPQSGWLWVRAALDREAQELYILKVMAVSGSKAELGQQTGTATVRVSILNQNEHSPRLSEDPTFLAVAENQPPGTSVGRVFATDRDSGPNGRLTYSLQQLSEDSKAFRIHPQTGEVTTLQTLDREQQSSYQLLVQVQDGGSPPRSTTGTVHVAVLDLNDNSPTFLQASGAAGGGLPIQVPDRVPPGTLVTTLQAKDPDEGENGTILYTLTGPGSELFSLHPHSGELLTAAPLIRAERPHYVLTLSAHDQGSPPRSASLQLLVQVLPSARLAEPPPDLAERDPAAPVPVVLTVTAAEGLRPGSLLGSVAAPEPAGVGALTYTLVGGADPEGTFALDAASGRLYLARPLDFEAGPPWRALTVRAEGPGGAGARLLRVQVQVQDENEHAPAFARDPLALALPENPEPGAALYTFRASDADGPGPNSDVRYRLLRQEPPVPALRLDARTGALSAPRGLDRETTPALLLLVEATDRPANASRRRAARVSARVFVTDENDNAPVFASPSRVRLPEDQPPGPAALHVVARDPDLGEAARVSYRLASGGDGHFRLHSSTGALSVVRPLDREQRAEHVLTVVASDHGSPPRSATQVLTVSVADVNDEAPTFQQQEYSVLLRENNPPGTSLLTLRATDPDVGANGQVTYGGVSSESFSLDPDTGVLTTLRALDREEQEEINLTVYAQDRGSPPQLTHVTVRVAVEDENDHAPTFGSAHLSLEVPEGQDPQTLTMLRASDPDVGANGQLQYRILDGDPSGAFVLDLASGEFGTMRPLDREVEPAFQLRIEARDGGQPALSATLLLTVTVLDANDHAPAFPVPAYSVEVPEDVPAGTLLLQLQAHDPDAGANGHVTYYLGAGTAGAFLLEPSSGELRTAAALDREQCPSYTFSVSAVDGAAAGPLSTTVSVTITVRDVNDHAPTFPTSPLRLRLPRPGPSFSTPTLALATLRAEDRDAGANASILYRLAGTPPPGTTVDSYTGEIRVARSPVALGPRDRVLFIVATDLGRPARSATGVIIVGLQGEAERGPRFPRASSEATIRENAPPGTPIVSPRAVHAGGTNGPITYSILSGNEKGTFSIQPSTGAITVRSAEGLDFEVSPRLRLVLQAESGGAFAFTVLTLTLQDANDNAPRFLRPHYVAFLPESRPLEGPLLQVEADDLDQGSGGQISYSLAASQPARGLFHVDPTTGTITTTAILDREIWAETRLVLMATDRGSPALVGSATLTVMVIDTNDNRPTIPQPWELRVSEDALLGSEIAQVTGNDVDSGPVLWYVLSPSGPQDPFSVGRYGGRVSLTGPLDFEQCDRYQLQLLAHDGPHEGRANLTVLVEDVNDNAPAFSQSLYQVMLLEHTPPGSAILSVSATDRDSGANGHISYHLASPADGFSVDPNNGTLFTIVGTVALGHDGSGAVDVVLEARDHGAPGRAARATVHVQLQDQNDHAPSFTLSHYRVAVTEDLPPGSTLLTLEATDADGSRSHAAVDYSIISGNWGRVFQLEPRLAEAGESAGPGPRALGCLVLLEPLDFESLTQYNLTVAAADRGQPPQSSVVPVTVTVLDVNDNPPVFTRASYRVTVPEDTPVGAELLHVEASDADPGPHGLVRFTVSSGDPSGLFELDESSGTLRLAHALDCETQARHQLVVQAADPAGAHFALAPVTIEVQDVNDHGPAFPLNLLSTSVAENQPPGTLVTTLHAIDGDAGAFGRLRYSLLEAGPGPEGREAFALNSSTGELRARVPFDYEHTESFRLLVGAADAGNLSASVTVSVLVTGEDEYDPVFLAPAFHFQVPEGARRGHSLGHVQATDEDGGADGLVLYSLATSSPYFGINQTTGALYLRVDSRAPGSGTATSGGGGRTRREAPRELRLEVIARGPLPGSRSATVPVTVDITHTALGLAPDLNLLLVGAVAASLGVVVVLALAALVLGLVRARSRKAEAAPGPMSQAAPLASDSLQKLGREPPSPPPSEHLYHQTLPSYGGPGAGGPYPRGGSLDPSHSSGRGSAEAAEDDEIRMINEFPRVASVASSLAARGPDSGIQQDADGLSDTSCEPPAPDTWYKGRKAGLLLPGAGATLYREEGPPATATAFLGGCGLSPAPTGDYGFPADGKPCVAGALTAIVAGEEELRGSYNWDYLLSWCPQFQPLASVFTEIARLKDEARPCPPAPRIDPPPLITAVAHPGAKSVPPKPANTAAARAIFPPASHRSPISHEGSLSSAAMSPSFSPSLSPLAARSPVVSPFGVAQGPSASALSAESGLEPPDDTELHI

Target ORF information:

RefSeq Version	NM_003737.4
Organism	Homo sapiens(human)
Definition	Homo sapiens dachsous cadherin-related 1 (DCHS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003737.4

ORF Insert Sequence:

ATGCAGAAGG AGCTGGGCAT TGTGCCTTCC TGCCCTGGCA TGAAGAGCCC CAGGCCCCAC 
CTCCTGCTAC CATTGCTGCT GCTGCTGCTG CTGCTGCTGG GGGCTGGGGT GCCAGGTGCC 
TGGGGTCAGG CTGGGAGCCT GGACTTGCAG ATTGATGAGG AGCAGCCAGC GGGTACACTG 
ATTGGCGACA TCAGTGCGGG GCTTCCGGCA GGCACGGCAG CTCCTCTCAT GTACTTCATC 
TCTGCCCAAG AGGGCAGCGG CGTGGGCACA GACCTGGCCA TTGACGAACA CAGTGGGGTC 
GTCCGTACAG CCCGTGTCTT GGACCGTGAG CAGCGGGACC GCTACCGCTT CACTGCAGTC 
ACTCCTGATG GTGCCACCGT AGAAGTTACA GTGCGAGTGG CTGACATCAA CGACCATGCT 
CCAGCCTTCC CACAGGCTCG GGCTGCCCTG CAGGTACCTG AGCATACAGC TTTTGGCACC 
CGCTACCCAC TGGAGCCTGC TCGTGATGCA GATGCTGGGC GTCTGGGAAC CCAGGGCTAT 
GCGCTATCTG GTGATGGGGC TGGAGAGACC TTCCGGCTGG AGACACGCCC CGGTCCAGAT 
GGGACTCCAG TACCTGAGCT GGTAGTTACT GGGGAACTGG ACCGAGAGAA CCGCTCACAC 
TATATGCTAC AGCTGGAGGC CTATGATGGT GGTTCACCCC CCCGGAGGGC CCAGGCCCTG 
CTGGACGTGA CACTGCTGGA CATCAATGAC CATGCCCCGG CTTTCAATCA GAGCCGCTAC 
CATGCTGTGG TGTCTGAGAG CCTGGCCCCT GGCAGTCCTG TCTTGCAGGT GTTCGCATCT 
GATGCCGATG CTGGTGTCAA TGGGGCTGTG ACTTACGAGA TCAACCGGAG GCAGAGCGAG 
GGTGATGGAC CCTTCTCCAT CGACGCACAC ACGGGGCTGC TGCAGTTAGA GCGGCCACTG 
GACTTTGAGC AGCGGCGGGT CCATGAACTG GTGGTGCAAG CACGAGATGG TGGGGCTCAC 
CCTGAGCTGG GCTCGGCCTT TGTGACTGTG CATGTGCGAG ATGCCAATGA CAATCAGCCC 
TCCATGACTG TCATCTTTCT CAGTGCAGAT GGCTCCCCCC AAGTGTCTGA GGCCGCCCCA 
CCTGGACAGC TCGTTGCTCG CATCTCTGTG TCAGACCCAG ATGATGGTGA CTTTGCCCAT 
GTCAATGTGT CCCTGGAAGG TGGAGAGGGC CACTTTGCCC TAAGCACCCA AGACAGCGTC 
ATCTATCTGG TGTGTGTGGC TCGGCGGCTG GATCGAGAGG AGAGGGATGC CTATAACTTG 
AGGGTTACAG CCACAGACTC AGGCTCACCT CCACTGCGGG CTGAGGCTGC CTTTGTGCTG 
CACGTCACTG ATGTCAACGA CAATGCACCT GCCTTTGACC GCCAGCTCTA CCGACCTGAG 
CCCCTGCCTG AGGTTGCGCT GCCTGGCAGC TTTGTAGTGC GGGTGACTGC TCGGGATCCT 
GACCAAGGCA CCAATGGTCA GGTCACTTAT AGCCTAGCCC CTGGCGCCCA CACCCACTGG 
TTCTCCATTG ACCCCACCTC AGGCATTATC ACTACGGCTG CCTCACTGGA CTATGAGTTG 
GAACCTCAGC CACAGCTGAT TGTGGTGGCC ACAGATGGTG GCCTGCCCCC TCTAGCCTCC 
TCTGCCACAG TTAGCGTGGC CCTGCAAGAT GTGAATGATA ATGAGCCCCA ATTCCAGAGG 
ACTTTCTACA ATGCCTCACT GCCTGAGGGC ACCCAGCCTG GAACTTGCTT CCTGCAGGTG 
ACAGCCACAG ACGCGGATAG TGGCCCATTT GGCCTCCTCT CCTATTCCTT GGGTGCTGGA 
CTTGGGTCCT CCGGATCTCC CCCATTCCGC ATTGATGCCC ACAGCGGTGA TGTGTGCACA 
ACCCGGACCC TGGACCGTGA CCAGGGGCCC TCAAGCTTTG ACTTCACAGT GACAGCTGTG 
GATGGGGGAG GCCTCAAGTC CATGGTATAT GTGAAGGTGT TTCTGTCAGA CGAGAATGAC 
AACCCTCCTC AGTTTTATCC ACGGGAGTAT GCTGCCAGTA TAAGTGCCCA GAGTCCACCA 
GGCACAGCTG TGCTGAGGTT GCGTGCCCAT GACCCTGACC AGGGATCCCA TGGGCGACTC 
TCCTACCATA TCCTGGCTGG CAACAGCCCC CCACTTTTTA CCTTGGATGA GCAATCAGGG 
CTGTTGACAG TAGCCTGGCC CTTGGCCAGA CGGGCCAATT CTGTGGTGCA GCTGGAGATC 
GGGGCTGAGG ACGGAGGTGG CCTACAGGCA GAACCCAGTG CCCGAGTGGA CATCAGCATT 
GTGCCTGGAA CCCCCACACC ACCCATATTT GAGCAACTAC AGTATGTTTT TTCTGTGCCA 
GAGGATGTGG CACCAGGCAC CAGTGTGGGC ATAGTCCAGG CACACAACCC ACCAGGTCGC 
TTGGCACCTG TGACCCTTTC CCTATCAGGT GGGGATCCCC GAGGACTCTT CTCCCTAGAT 
GCGGTATCAG GACTGTTGCA AACACTTCGC CCTCTGGACC GGGAGCTACT GGGACCAGTG 
TTGGAGCTGG AGGTGCGAGC AGGCAGTGGA GTGCCCCCAG CTTTCGCTGT AGCTCGGGTG 
CGTGTGCTGC TGGATGATGT GAATGACAAC TCCCCTGCCT TTCCTGCACC TGAAGACACG 
GTATTGCTAC CACCAAACAC TGCCCCAGGG ACTCCCATCT ATACACTGCG GGCTCTTGAC 
CCCGACTCAG GTGTTAACAG TCGAGTCACC TTTACCCTGC TTGCTGGGGG TGGTGGAGCC 
TTCACCGTGG ACCCCACCAC AGGCCATGTA CGGCTTATGA GGCCTCTGGG GCCCTCAGGA 
GGGCCAGCCC ATGAGCTGGA GCTGGAGGCC CGGGATGGGG GCTCCCCACC ACGCACCAGC 
CACTTTCGAC TACGGGTGGT GGTACAGGAT GTGGGAACCC GTGGGCTGGC TCCCCGATTC 
AACAGCCCTA CCTACCGTGT GGACCTGCCC TCAGGCACCA CTGCTGGAAC TCAGGTCCTG 
CAAGTGCAGG CCCAAGCACC AGATGGGGGC CCTATCACCT ATCACCTTGC AGCAGAGGGA 
GCAAGTAGCC CCTTTGGCCT GGAGCCACAG AGTGGGTGGC TATGGGTGCG GGCAGCACTA 
GACCGTGAGG CCCAGGAATT GTACATACTG AAGGTAATGG CAGTGTCTGG GTCCAAAGCT 
GAGTTGGGGC AGCAGACAGG CACAGCCACC GTGAGGGTCA GCATCCTCAA CCAGAATGAA 
CACAGTCCCC GCTTGTCTGA GGATCCCACC TTCCTGGCTG TGGCTGAGAA CCAGCCCCCA 
GGGACCAGCG TGGGCCGAGT CTTTGCCACT GACCGAGACT CAGGACCCAA TGGACGTCTG 
ACCTACAGCC TGCAACAGCT GTCTGAAGAC AGCAAGGCCT TCCGCATCCA CCCCCAGACT 
GGAGAAGTGA CCACACTCCA AACCCTGGAC CGTGAGCAGC AGAGCAGCTA TCAGCTCCTG 
GTGCAGGTGC AGGATGGAGG GAGCCCACCC CGCAGCACCA CAGGCACTGT GCATGTTGCA 
GTGCTTGACC TCAACGACAA CAGCCCCACG TTCCTGCAGG CTTCAGGAGC TGCTGGTGGG 
GGCCTCCCTA TACAGGTACC AGACCGCGTG CCTCCGGGAA CACTGGTGAC GACTCTGCAG 
GCGAAGGATC CAGATGAGGG GGAGAATGGG ACCATCTTGT ACACGCTAAC TGGTCCTGGC 
TCAGAGCTTT TCTCTCTGCA CCCTCACTCA GGGGAGCTGC TCACTGCAGC TCCCCTGATC 
CGAGCAGAGC GGCCCCACTA TGTGCTGACA CTGAGTGCTC ATGACCAAGG CAGCCCTCCT 
CGAAGTGCCA GCCTCCAGCT GCTGGTGCAG GTGCTTCCCT CAGCTCGCTT GGCCGAGCCG 
CCCCCAGATC TCGCAGAGCG GGACCCAGCG GCACCAGTGC CTGTCGTGCT GACGGTGACA 
GCAGCTGAGG GACTGCGGCC CGGCTCTCTG TTGGGCTCGG TGGCAGCGCC AGAGCCCGCG 
GGTGTGGGTG CACTCACCTA CACACTGGTG GGCGGTGCCG ATCCCGAGGG CACCTTCGCG 
CTGGATGCGG CCTCAGGGCG CTTGTACCTG GCGCGGCCCC TGGACTTCGA AGCTGGCCCG 
CCGTGGCGCG CGCTTACGGT ACGCGCTGAG GGGCCGGGAG GCGCGGGCGC GCGGCTGCTG 
CGAGTGCAGG TGCAAGTGCA GGACGAGAAT GAGCATGCGC CCGCCTTTGC GCGCGACCCG 
CTGGCGCTGG CGCTGCCAGA GAACCCGGAG CCCGGCGCAG CGCTGTACAC TTTCCGCGCG 
TCGGACGCCG ACGGCCCCGG CCCCAATAGC GACGTGCGCT ACCGCCTGCT GCGCCAGGAG 
CCGCCCGTGC CGGCGCTTCG CCTGGACGCG CGCACCGGGG CGCTCAGCGC TCCGCGCGGC 
CTGGACCGAG AGACCACTCC CGCGCTGCTG CTGCTGGTGG AAGCCACCGA CCGGCCCGCC 
AACGCCAGCC GCCGTCGTGC AGCGCGCGTT TCAGCGCGCG TCTTCGTCAC GGATGAGAAT 
GACAACGCGC CTGTCTTCGC CTCGCCGTCA CGCGTGCGCC TCCCAGAGGA CCAGCCGCCT 
GGGCCCGCGG CCCTGCACGT GGTAGCCCGG GACCCGGATC TGGGCGAGGC TGCACGCGTG 
TCCTATCGGC TGGCATCTGG CGGGGACGGC CACTTCCGGC TGCACTCAAG CACTGGAGCG 
CTGTCCGTGG TGCGGCCGTT GGACCGCGAA CAACGAGCTG AGCACGTACT GACAGTGGTG 
GCCTCAGACC ACGGCTCCCC GCCGCGCTCG GCCACGCAGG TCCTGACCGT CAGTGTCGCT 
GACGTCAACG ACGAGGCGCC TACTTTCCAG CAGCAGGAGT ACAGCGTCCT CTTGCGTGAG 
AACAACCCTC CTGGCACATC TCTGCTCACC CTGCGAGCAA CCGACCCCGA CGTGGGGGCC 
AACGGGCAAG TGACTTATGG AGGCGTCTCT AGCGAAAGCT TTTCTCTGGA TCCTGACACT 
GGTGTTCTCA CGACTCTTCG GGCCCTGGAT CGAGAGGAAC AGGAGGAGAT CAACCTGACA 
GTGTATGCCC AGGACAGGGG CTCACCTCCT CAGTTAACGC ATGTCACTGT TCGAGTGGCT 
GTGGAGGATG AGAATGACCA TGCACCAACC TTTGGGAGTG CCCATCTCTC TCTGGAGGTG 
CCTGAGGGCC AGGACCCCCA GACCCTTACC ATGCTTCGGG CCTCTGATCC AGATGTGGGA 
GCCAATGGGC AGTTGCAGTA CCGCATCCTA GATGGGGACC CATCAGGAGC CTTTGTCCTA 
GACCTTGCTT CTGGAGAGTT TGGCACCATG CGGCCACTAG ACAGAGAAGT GGAGCCAGCT 
TTCCAGCTGA GGATAGAGGC CCGGGATGGA GGCCAGCCAG CTCTCAGTGC CACGCTGCTT 
TTGACAGTGA CAGTGCTGGA TGCCAATGAC CATGCTCCAG CCTTTCCTGT GCCTGCCTAC 
TCGGTGGAGG TGCCGGAGGA TGTGCCTGCA GGGACCCTGC TGCTGCAGCT ACAGGCTCAT 
GACCCTGATG CTGGAGCTAA TGGCCATGTG ACCTACTACC TGGGCGCCGG TACAGCAGGA 
GCCTTCCTGC TGGAGCCCAG CTCTGGAGAA CTGCGCACAG CTGCAGCCTT GGACAGAGAA 
CAGTGTCCCA GCTACACCTT TTCTGTGAGT GCAGTGGATG GTGCAGCTGC TGGGCCCCTA 
AGCACCACAG TGTCTGTCAC CATCACGGTG CGCGATGTCA ATGACCATGC ACCCACCTTC 
CCCACCAGTC CTCTGCGCCT ACGTCTGCCC CGCCCAGGCC CCAGCTTCAG TACCCCAACC 
CTGGCTCTGG CCACACTGAG AGCTGAAGAT CGTGATGCTG GTGCCAATGC TTCCATTCTG 
TACCGGCTGG CAGGCACACC ACCTCCTGGC ACTACTGTGG ACTCTTACAC TGGTGAAATC 
CGCGTGGCCC GCTCTCCTGT AGCTCTAGGC CCCCGAGATC GTGTCCTCTT CATTGTGGCC 
ACTGATCTTG GCCGTCCAGC TCGCTCTGCC ACTGGTGTGA TCATTGTTGG ACTGCAGGGG 
GAAGCTGAGC GTGGACCCCG CTTTCCCCGG GCTAGCAGTG AGGCTACGAT TCGTGAGAAT 
GCGCCCCCAG GGACTCCTAT TGTCTCCCCC AGGGCCGTCC ATGCAGGAGG CACAAATGGA 
CCCATCACCT ACAGCATTCT CAGTGGGAAT GAGAAAGGGA CATTCTCCAT CCAGCCTAGT 
ACAGGTGCCA TCACAGTTCG CTCAGCAGAG GGGCTAGACT TCGAGGTGAG TCCACGGCTG 
CGACTGGTGC TGCAGGCAGA GAGTGGAGGA GCCTTTGCCT TCACTGTGCT GACCCTGACC 
CTGCAAGATG CCAACGACAA TGCTCCCCGT TTCCTGCGGC CCCATTATGT GGCCTTCCTT 
CCTGAGTCCC GGCCCTTGGA GGGGCCCCTG CTGCAGGTGG AGGCGGATGA CCTGGATCAA 
GGCTCTGGAG GACAGATTTC CTACAGTCTG GCTGCATCCC AGCCGGCACG TGGATTGTTC 
CACGTAGACC CAACCACAGG CACTATCACT ACCACAGCCA TCCTGGACCG TGAGATCTGG 
GCTGAAACAC GGTTGGTGCT GATGGCCACA GACAGAGGGA GCCCAGCCCT GGTGGGCTCA 
GCTACCTTGA CGGTGATGGT CATCGACACC AATGACAATC GCCCCACCAT CCCCCAACCC 
TGGGAGCTCC GAGTGTCAGA AGATGCGTTA TTGGGCTCAG AGATTGCACA GGTAACAGGG 
AATGATGTGG ACTCAGGACC CGTGCTGTGG TATGTGCTAA GCCCATCTGG GCCCCAGGAT 
CCCTTCAGTG TTGGCCGCTA TGGAGGCCGT GTCTCCCTCA CGGGGCCCCT GGACTTTGAG 
CAGTGTGACC GCTACCAGCT GCAGCTGCTG GCACATGATG GGCCTCATGA GGGCCGTGCC 
AACCTCACAG TGCTTGTGGA GGATGTCAAT GACAATGCAC CTGCCTTCTC ACAGAGCCTC 
TACCAGGTAA TGCTGCTTGA GCACACACCC CCAGGCAGTG CCATTCTCTC CGTCTCTGCC 
ACTGATCGGG ACTCAGGTGC CAACGGTCAC ATTTCCTACC ACCTGGCTTC CCCTGCCGAT 
GGCTTCAGTG TTGACCCCAA CAATGGGACC CTGTTCACAA TAGTGGGAAC AGTGGCCTTG 
GGCCATGACG GGTCAGGAGC AGTGGATGTG GTGCTGGAAG CACGAGACCA CGGGGCTCCA 
GGCCGGGCAG CACGAGCCAC AGTGCACGTG CAGCTGCAGG ACCAGAACGA CCACGCCCCG 
AGCTTCACAT TGTCACACTA CCGTGTGGCT GTGACTGAAG ACCTGCCCCC TGGCTCCACT 
CTGCTCACCC TGGAGGCTAC AGATGCTGAT GGAAGCCGCA GCCATGCCGC TGTGGACTAC 
AGCATCATCA GTGGCAACTG GGGCCGAGTC TTCCAGCTGG AACCCAGGCT GGCTGAGGCT 
GGGGAGAGTG CTGGACCAGG CCCCCGGGCA CTGGGCTGCC TGGTGTTGCT TGAACCTCTA 
GACTTTGAAA GCCTGACACA GTACAATCTA ACAGTGGCTG CAGCTGACCG TGGGCAGCCA 
CCCCAAAGCT CAGTCGTGCC AGTCACTGTC ACTGTACTAG ATGTCAATGA CAACCCACCT 
GTCTTTACCC GAGCATCCTA CCGTGTGACA GTACCTGAGG ACACACCTGT TGGAGCTGAG 
CTGCTGCATG TAGAGGCCTC TGACGCTGAC CCTGGCCCTC ATGGCCTCGT GCGTTTCACT 
GTCAGCTCAG GCGACCCATC AGGGCTCTTT GAGCTGGATG AGAGCTCAGG CACCTTGCGA 
CTGGCCCATG CCCTGGACTG TGAGACCCAG GCTCGACATC AGCTTGTAGT ACAGGCTGCT 
GACCCTGCTG GTGCACACTT TGCTTTGGCA CCAGTGACAA TTGAGGTCCA GGATGTGAAT 
GATCATGGCC CAGCCTTCCC ACTGAACTTA CTCAGCACCA GCGTGGCCGA GAATCAGCCT 
CCAGGCACTC TCGTGACCAC TCTGCATGCA ATCGACGGGG ATGCTGGGGC TTTTGGGAGG 
CTCCGTTACA GCCTGTTGGA GGCTGGGCCA GGACCTGAGG GCCGTGAGGC ATTTGCACTG 
AACAGCTCAA CAGGGGAGTT GCGTGCGCGA GTGCCCTTTG ACTATGAGCA CACAGAAAGC 
TTCCGGCTGC TGGTGGGTGC TGCTGATGCT GGGAATCTCT CAGCCTCTGT CACTGTGTCG 
GTGCTAGTGA CTGGAGAGGA TGAGTATGAC CCTGTATTTC TGGCACCAGC TTTCCACTTC 
CAAGTGCCCG AAGGTGCCCG GCGTGGCCAC AGCTTGGGTC ACGTGCAGGC CACAGATGAG 
GATGGGGGTG CCGATGGCCT GGTTCTGTAT TCCCTTGCCA CCTCTTCCCC CTATTTTGGT 
ATTAACCAGA CTACAGGAGC CCTGTACCTG CGGGTGGACA GTCGGGCACC AGGCAGCGGA 
ACAGCCACCT CTGGGGGTGG GGGCCGGACC CGGCGGGAAG CACCACGGGA GCTGAGGCTG 
GAGGTGATAG CACGGGGGCC TCTGCCTGGT TCCCGGAGTG CCACAGTGCC TGTGACCGTG 
GATATCACCC ACACCGCACT GGGCCTGGCA CCTGACCTCA ACCTGCTATT AGTAGGGGCC 
GTGGCAGCCT CCTTGGGAGT TGTGGTGGTG CTTGCACTGG CAGCCCTGGT CCTAGGACTT 
GTTCGGGCCC GTAGCCGCAA GGCTGAGGCA GCCCCTGGCC CAATGTCACA GGCAGCACCC 
CTAGCCAGTG ACTCACTGCA GAAACTGGGC CGGGAGCCAC CTAGTCCACC ACCCTCTGAG 
CACCTCTATC ACCAGACTCT TCCCAGCTAT GGTGGGCCAG GAGCTGGAGG ACCCTACCCC 
CGTGGTGGCT CCTTGGACCC TTCACATTCA AGTGGCCGAG GATCAGCAGA GGCTGCAGAG 
GATGATGAGA TCCGCATGAT CAATGAGTTC CCCCGTGTGG CCAGTGTGGC CTCCTCTCTG 
GCTGCCCGTG GCCCTGACTC AGGCATCCAG CAGGATGCAG ATGGTCTGAG TGACACATCC 
TGCGAACCAC CTGCCCCTGA CACCTGGTAT AAGGGCCGAA AGGCAGGGCT GCTGCTGCCA 
GGTGCAGGAG CCACTCTCTA CAGAGAGGAG GGGCCCCCAG CCACTGCCAC AGCCTTCCTG 
GGGGGCTGTG GCCTGAGCCC TGCACCCACT GGGGACTATG GCTTCCCAGC AGATGGCAAG 
CCATGTGTGG CAGGTGCGCT GACAGCCATT GTGGCCGGCG AGGAGGAGCT CCGTGGCAGC 
TATAACTGGG ACTACCTGCT GAGCTGGTGC CCTCAGTTCC AACCACTGGC CAGTGTCTTC 
ACAGAGATCG CTCGGCTCAA GGATGAAGCT CGGCCATGTC CCCCAGCTCC CCGTATCGAC 
CCACCACCCC TCATCACTGC CGTGGCCCAC CCAGGAGCCA AGTCTGTGCC CCCCAAGCCA 
GCAAACACAG CTGCAGCCCG GGCCATCTTC CCACCAGCTT CTCACCGCTC CCCCATCAGC 
CATGAAGGCT CCCTGTCCTC AGCTGCCATG TCCCCCAGCT TCTCACCCTC TCTGTCTCCT 
CTGGCTGCTC GCTCACCCGT TGTCTCACCA TTTGGGGTGG CCCAGGGTCC CTCAGCCTCA 
GCACTCAGCG CAGAGTCTGG CCTGGAGCCA CCTGATGACA CGGAGCTGCA CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deleterious variants in DCHS1 are prevalent in sporadic cases of mitral valve prolapse.
Molecular genetics & genomic medicine(2017 Dec)
Clemenceau A,B?rub? JC,B?langer P,Gaudreault N,Lamontagne M,Toubal O,Clavel MA,Capoulade R,Mathieu P,Pibarot P,Bosse Y

Mammalian cadherins DCHS1-FAT4 affect functional cerebral architecture.
Brain structure & function221(5)2487-91(2016 06)
Beste C,Ocklenburg S,von der Hagen M,Di Donato N

The atypical cadherin Dachsous1 localizes to the base of the ciliary apparatus in airway epithelia.
Biochemical and biophysical research communications473(4)1177-1184(2016 05)
Dau C,Fliegauf M,Omran H,Schlensog M,Dahl E,van Roeyen CR,Kriz W,Moeller MJ,Braun GS

Mutations in DCHS1 cause mitral valve prolapse.
Nature525(7567)109-13(2015 Sep)
Durst R,Sauls K,Peal DS,deVlaming A,Toomer K,Leyne M,Salani M,Talkowski ME,Brand H,Perrocheau M,Simpson C,Jett C,Stone MR,Charles F,Chiang C,Lynch SN,Bouatia-Naji N,Delling FN,Freed LA,Tribouilloy C,Le Tourneau T,LeMarec H,Fernandez-Friera L,Solis J,Trujillano D,Ossowski S,Estivill X,Dina C,Bruneval P,Chester A,Schott JJ,Irvine KD,Mao Y,Wessels A,Motiwala T,Puceat M,Tsukasaki Y,Menick DR,Kasiganesan H,Nie X,Broome AM,Williams K,Johnson A,Markwald RR,Jeunemaitre X,Hagege A,Levine RA,Milan DJ,Norris RA,Slaugenhaupt SA

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DCHS1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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