TOPBP1 cDNA ORF clone, Canis lupus familiaris(dog)

The following TOPBP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOPBP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe296512 XM_022408654.1
Latest version!
Canis lupus familiaris DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe24161 XM_022408653.1
Latest version!
Canis lupus familiaris DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24161 XM_534266.6
Latest version!
Canis lupus familiaris DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24162 XM_005634445.3
Latest version!
Canis lupus familiaris DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24163 XM_005634446.3
Latest version!
Canis lupus familiaris DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24164 XM_005634447.3
Latest version!
Canis lupus familiaris DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe24165 XM_005634448.2
Latest version!
Canis lupus familiaris DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OCe24165 XM_005634448.1 Canis lupus familiaris topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe24161 XM_534266.5 Canis lupus familiaris topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24162 XM_005634445.2 Canis lupus familiaris topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24163 XM_005634446.2 Canis lupus familiaris topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24164 XM_005634447.2 Canis lupus familiaris topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe296512
    Clone ID Related Accession (Same CDS sequence) XM_022408654.1
    Accession Version XM_022408654.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4368bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product DNA topoisomerase 2-binding protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCAGAA ATGACAAAGA ACCGTTTCTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    GAATGTTTTT TTAAAGCTCT GGAGTCAATA AAAGAATTTC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA CAGGAAAATG ATAGGTCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGC
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAACGATGTG TCCCAAGAGC TGAACATCCA
    GTCTATAATA TGGTTATGTC TGATGTAACT ATATCTTGTA CAAGCCTGGA AAAAGACAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GTGTATATAG AGATCTTAAT
    ATATCAGTAA CTCATCTTAT TGCAGGAGAA GTTGGCAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCGATTTT GCTTCCTTCT TGGATTAAAA CACTTTGGGA AAAGTCACAA
    GAGAAAAAAA TATCTAGATA TACTGATGTA AACATGGAAG ACTTCAAGTG CCCAATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGGTTG TGTAGCTTAG ATAGGAAGGC AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATATATG GGACAACTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT GTTGACAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ACCAGAAACA AAGACTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCC ACACAGTTGA CAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGTT GCATAAATGA ATCAATGTGT AATTCAGTGC TTAATAGCAA AGTGGAGCCT
    ACCCTTGAAA ATCTAGAAAA TTTGGATGTC AGTGCATTTC AAGCACCTGA AGATTTATTA
    GATGGTTGTC GGATCTATCT TTGCGGTTTT AGTGGCAGAA GGCTAGATAA ACTAAGAAGG
    CTTATTAACA GTGGAGGTGG AGTTCGTTTT AATCAGCTCA ATGAAGATGT AACTCATGTT
    ATAGTGGGAG ATTATGATGA TGAATTGAAG CAGTTTTGGG ATAAATCGGC CCACAGGCCT
    CATGTAGTAG GAGCAAAATG GTTGCTGGAG TGTTTTAGTA AAGGTTATAT GCTTCCTGAA
    GAAACATATA TCCATGCTAA CTACCAGCCA GTGGAAATTC CAGTTTCAGA TCAACCTGAA
    AATAAAACAG CCCTTTTAAA AAAGAACAAC AGCTTTTCTA AGAAAGACTT TGCTTCTGAT
    GAAAAGCATG AACAAGCTGA TGAAGATCTG CTCTCTCAAT ATGTAAATAA TAACCCGACA
    GTAGGTGAAG CTGTGAAGTC TGAAGTTGAG CCCTTTAATG ACTCTACTCA TATTTCTCCG
    CAAAAAGAAA ACCAGTCTTC TGCCACTCAT TGCCTCCCTG ATGCTTCTAC AACTACTGAA
    GAAGGTTTAT TTAGCCAAAA GAGTTTCCTT GTTTTGGGGT TTAGTAATGA AAATGAATCT
    AATATTGCAA ATATCATAAG AGAAAATGCT GGAAAAGTCG TGTCCCTTCA GAGCAGAATT
    GTTGCTGATT ATGCTGTGGT TCCTCTGCTG GGGTGTGAAG TAGAAGCAAC TGTAGGAGAA
    GTCGTCACAA ATACATGGCT GGTAACTTGT ATAGACTATC AGACTTTAAT TGATCCAAAG
    TCAAATCCTC TCTTCATGCC AGTTCCATTT ATGGCAGGAA TGACTCCTTT AGAGGATTGT
    GTTATTTCAT TCAGCCAGTG TGCAGGAGCA GAAAAAGATT CATTGACATT CTTAGCAAAT
    CGCCTTGGAG CTAGCGTTCA AGAATTCTTT GTTCGCAAAT CCAATGCAAA GAAAGGCATG
    TTTGCCAGTA CACATCTTGT ATTGAAAGAA CCAGGTGGTT CAAAATATGA AGCTGCAAAG
    AAGTGGAATT TGCCAGCAGT CACCATATCT TGGCTGTTGG AGACTGCTAG AATGGGAAAG
    AGAGCAAATG AAAACCATTT TCTGATTGAA AAGTCAACTA AAGAAGAACA AAGTTTGGAA
    ACTGAAATAA CAAACAAGGT GAATCTAAAT CCAGATACTC CAGAGCATCC TGTTGCACAC
    CTGGAAACTC AGAGGAAAAC AGCTGTTACA CCTTTAGATA TGAACCGCTT TCAGAGTAGA
    GCTTTCCATG CTGTGATCTC ACAACACACC AAACAGGTTT CGACCTCCTC GCCAGTGGGA
    CAGCCACTTC AGAAGGAGCC CTCCTTACAT CTGGACACAC CATCAAAGTT TCTGTCCAAG
    GACAAACTCT TCAAGCCTTC CTTTGATGTG AAGGATGAAG CTCCAAAGCC ACTTCATAAG
    GTAGTGGTAT GTGTGAGTAA GAAACTCAGT AAGAAGCAGA GTGAACTGAA TGGGATCGCA
    GCCTCTTTAG GAGCAGATTA CAGATGGAGT TTTGATGAAA CCGTGACTCA TTTCATTTAT
    CAAGGCCGAC CAAATGACAC TAACCGAGAG TATAAATCTG TAAAAGAAAG AGGAGTACAC
    ATTGTTTCAG AGCACTGGCT TTTAGAATGT GCCCAAGAGT ATAAACATCT TCCTGAATCT
    CTTTATCCAC ATACTTATAA TCCCAAAATG AGCTTGGATA TCAGTACAGT GCAAGATGGC
    AGAATCTCTA ATAGTCGACT GCTCTCAGCT GATTCAACGA CAAAGGATGA TGAGCCAGAT
    CATTTGCCTG GAGAAGAAAA CGATATAGAC AATATGACCA CCAGTAATAA AGAATCCGCA
    ACATCAAATG GAGATGGAAG GAATGACTCT AAAGGAGCTC TGACACAGAC TTTGGAGATG
    AGAGAGAATT TTCAAAAGCA GCTGCAAGAG ATAATGTCTG CAACATCAAT AGTGAAACCT
    CAGGGACCAA GGGCTTCCCT TTCAAGAAGT GGTTGTAACA GTGCTTCTTC AACCCCTGAT
    AGCACTCGCT CTGCCCGCAG TGGGCGAAGT AGAGTCCTGG AGGCACTGAG GCAGTCTCGT
    CAAACGGTAC CTGATATCAA CACAGAGCCC TCCCAGAATG AACAGATCAT TTGGGATGAC
    CCGACAGCCA GGGAAGAGAG AGCAAGGCTT GCCAGCAATC TGCAGTGGCC TAGTTGTCCC
    ACACAGTATT CTGAGCTTCA GGTTGACATT AAAAAATTGG AGGATTCTCC TTTCCAGGAG
    CCTTTACATG ATCCAGAAAT TGCTGAACAG GCAGTCTGTG ATCCCGGAAA TGTGTGTGTG
    ACTGAAGCCC CAAAACACCC GACCTCTGAA GAACTGGAAA CACCAGTAAA AGACAGCCAC
    CTGATCCCTA CACCTCAAGC TCCCAGTATT GCCTTCCCCC TAGCCAACCC ACCTGTGGCT
    CCACACCCTA GAGAAAAGAT TATAACAATA GAGGAAACCC ATGAAGACTT GAAAAAACAG
    TACATATTTC AGTTGTCGTC TCTGAATCCT CAAGAACGTA TTGATTATTG TCATCTGATT
    GAAAAACTAG GTGGATTAGT AATAGAGAAG CAATGCTTTG ATCCCAACTG TACACACATT
    GTTGTGGGAC ATCCTCTTCG AAACGAGAAG TATTTAGCTT CAGTGGCAGC TGGGAAGTGG
    GTACTTCATC GCTCCTACCT GGAAGCATGC CGGACTGCTG GGCACTTCGT GCTGGAAGAA
    GACTATGAAT GGGGAAGTAG TTCCATACTT GATGTTTTGA CTGGAATCAA TGTACAGCAA
    CGAAGACTAG CACTTGCAGC AATGAGATGG AGGAAAAAAA TCCAGCAAAG ACAAGAATCA
    GGCATTGTTG AGGGGGCATT TAGTGGGTGG AAGGTTATCT TACATGTTGA CCAGTCCCGA
    GAAGCAGGAT TCAAACGCCT TCTTCAGTCA GGAGGAGCAA AGGTACTACC TGGTCATTCT
    GTACCTTTAT TTAAAGAAGC TACACATCTC TTTTCTGACT TCAATAAACT GAAACCAGAT
    GACTCAGGAG TTAATATAGC AGAAGCTGCT GCCCAAAATG TGTACTGCTT GAAAACAGAG
    TACATTGCTG ATTATCTGAT GCAGGAATCA CCTCCTCCAG TAGAAAATTA CTGTCTACCA
    GAAGCAGTTT CATTTCTTCA GAATAATAAG GAACCTGGGC CTGGATTATC ACAGAAGAGG
    AAAGCTCCTA CAGAGAAAAA TAAAATCAAA CGACCTAGAG TAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022264362.1
    CDS144..4511
    Translation

    Target ORF information:

    RefSeq Version XM_022408654.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022408654.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCAGAA ATGACAAAGA ACCGTTTCTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    GAATGTTTTT TTAAAGCTCT GGAGTCAATA AAAGAATTTC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA CAGGAAAATG ATAGGTCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGC
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAACGATGTG TCCCAAGAGC TGAACATCCA
    GTCTATAATA TGGTTATGTC TGATGTAACT ATATCTTGTA CAAGCCTGGA AAAAGACAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GTGTATATAG AGATCTTAAT
    ATATCAGTAA CTCATCTTAT TGCAGGAGAA GTTGGCAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCGATTTT GCTTCCTTCT TGGATTAAAA CACTTTGGGA AAAGTCACAA
    GAGAAAAAAA TATCTAGATA TACTGATGTA AACATGGAAG ACTTCAAGTG CCCAATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGGTTG TGTAGCTTAG ATAGGAAGGC AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATATATG GGACAACTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT GTTGACAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ACCAGAAACA AAGACTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCC ACACAGTTGA CAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGTT GCATAAATGA ATCAATGTGT AATTCAGTGC TTAATAGCAA AGTGGAGCCT
    ACCCTTGAAA ATCTAGAAAA TTTGGATGTC AGTGCATTTC AAGCACCTGA AGATTTATTA
    GATGGTTGTC GGATCTATCT TTGCGGTTTT AGTGGCAGAA GGCTAGATAA ACTAAGAAGG
    CTTATTAACA GTGGAGGTGG AGTTCGTTTT AATCAGCTCA ATGAAGATGT AACTCATGTT
    ATAGTGGGAG ATTATGATGA TGAATTGAAG CAGTTTTGGG ATAAATCGGC CCACAGGCCT
    CATGTAGTAG GAGCAAAATG GTTGCTGGAG TGTTTTAGTA AAGGTTATAT GCTTCCTGAA
    GAAACATATA TCCATGCTAA CTACCAGCCA GTGGAAATTC CAGTTTCAGA TCAACCTGAA
    AATAAAACAG CCCTTTTAAA AAAGAACAAC AGCTTTTCTA AGAAAGACTT TGCTTCTGAT
    GAAAAGCATG AACAAGCTGA TGAAGATCTG CTCTCTCAAT ATGTAAATAA TAACCCGACA
    GTAGGTGAAG CTGTGAAGTC TGAAGTTGAG CCCTTTAATG ACTCTACTCA TATTTCTCCG
    CAAAAAGAAA ACCAGTCTTC TGCCACTCAT TGCCTCCCTG ATGCTTCTAC AACTACTGAA
    GAAGGTTTAT TTAGCCAAAA GAGTTTCCTT GTTTTGGGGT TTAGTAATGA AAATGAATCT
    AATATTGCAA ATATCATAAG AGAAAATGCT GGAAAAGTCG TGTCCCTTCA GAGCAGAATT
    GTTGCTGATT ATGCTGTGGT TCCTCTGCTG GGGTGTGAAG TAGAAGCAAC TGTAGGAGAA
    GTCGTCACAA ATACATGGCT GGTAACTTGT ATAGACTATC AGACTTTAAT TGATCCAAAG
    TCAAATCCTC TCTTCATGCC AGTTCCATTT ATGGCAGGAA TGACTCCTTT AGAGGATTGT
    GTTATTTCAT TCAGCCAGTG TGCAGGAGCA GAAAAAGATT CATTGACATT CTTAGCAAAT
    CGCCTTGGAG CTAGCGTTCA AGAATTCTTT GTTCGCAAAT CCAATGCAAA GAAAGGCATG
    TTTGCCAGTA CACATCTTGT ATTGAAAGAA CCAGGTGGTT CAAAATATGA AGCTGCAAAG
    AAGTGGAATT TGCCAGCAGT CACCATATCT TGGCTGTTGG AGACTGCTAG AATGGGAAAG
    AGAGCAAATG AAAACCATTT TCTGATTGAA AAGTCAACTA AAGAAGAACA AAGTTTGGAA
    ACTGAAATAA CAAACAAGGT GAATCTAAAT CCAGATACTC CAGAGCATCC TGTTGCACAC
    CTGGAAACTC AGAGGAAAAC AGCTGTTACA CCTTTAGATA TGAACCGCTT TCAGAGTAGA
    GCTTTCCATG CTGTGATCTC ACAACACACC AAACAGGTTT CGACCTCCTC GCCAGTGGGA
    CAGCCACTTC AGAAGGAGCC CTCCTTACAT CTGGACACAC CATCAAAGTT TCTGTCCAAG
    GACAAACTCT TCAAGCCTTC CTTTGATGTG AAGGATGAAG CTCCAAAGCC ACTTCATAAG
    GTAGTGGTAT GTGTGAGTAA GAAACTCAGT AAGAAGCAGA GTGAACTGAA TGGGATCGCA
    GCCTCTTTAG GAGCAGATTA CAGATGGAGT TTTGATGAAA CCGTGACTCA TTTCATTTAT
    CAAGGCCGAC CAAATGACAC TAACCGAGAG TATAAATCTG TAAAAGAAAG AGGAGTACAC
    ATTGTTTCAG AGCACTGGCT TTTAGAATGT GCCCAAGAGT ATAAACATCT TCCTGAATCT
    CTTTATCCAC ATACTTATAA TCCCAAAATG AGCTTGGATA TCAGTACAGT GCAAGATGGC
    AGAATCTCTA ATAGTCGACT GCTCTCAGCT GATTCAACGA CAAAGGATGA TGAGCCAGAT
    CATTTGCCTG GAGAAGAAAA CGATATAGAC AATATGACCA CCAGTAATAA AGAATCCGCA
    ACATCAAATG GAGATGGAAG GAATGACTCT AAAGGAGCTC TGACACAGAC TTTGGAGATG
    AGAGAGAATT TTCAAAAGCA GCTGCAAGAG ATAATGTCTG CAACATCAAT AGTGAAACCT
    CAGGGACCAA GGGCTTCCCT TTCAAGAAGT GGTTGTAACA GTGCTTCTTC AACCCCTGAT
    AGCACTCGCT CTGCCCGCAG TGGGCGAAGT AGAGTCCTGG AGGCACTGAG GCAGTCTCGT
    CAAACGGTAC CTGATATCAA CACAGAGCCC TCCCAGAATG AACAGATCAT TTGGGATGAC
    CCGACAGCCA GGGAAGAGAG AGCAAGGCTT GCCAGCAATC TGCAGTGGCC TAGTTGTCCC
    ACACAGTATT CTGAGCTTCA GGTTGACATT AAAAAATTGG AGGATTCTCC TTTCCAGGAG
    CCTTTACATG ATCCAGAAAT TGCTGAACAG GCAGTCTGTG ATCCCGGAAA TGTGTGTGTG
    ACTGAAGCCC CAAAACACCC GACCTCTGAA GAACTGGAAA CACCAGTAAA AGACAGCCAC
    CTGATCCCTA CACCTCAAGC TCCCAGTATT GCCTTCCCCC TAGCCAACCC ACCTGTGGCT
    CCACACCCTA GAGAAAAGAT TATAACAATA GAGGAAACCC ATGAAGACTT GAAAAAACAG
    TACATATTTC AGTTGTCGTC TCTGAATCCT CAAGAACGTA TTGATTATTG TCATCTGATT
    GAAAAACTAG GTGGATTAGT AATAGAGAAG CAATGCTTTG ATCCCAACTG TACACACATT
    GTTGTGGGAC ATCCTCTTCG AAACGAGAAG TATTTAGCTT CAGTGGCAGC TGGGAAGTGG
    GTACTTCATC GCTCCTACCT GGAAGCATGC CGGACTGCTG GGCACTTCGT GCTGGAAGAA
    GACTATGAAT GGGGAAGTAG TTCCATACTT GATGTTTTGA CTGGAATCAA TGTACAGCAA
    CGAAGACTAG CACTTGCAGC AATGAGATGG AGGAAAAAAA TCCAGCAAAG ACAAGAATCA
    GGCATTGTTG AGGGGGCATT TAGTGGGTGG AAGGTTATCT TACATGTTGA CCAGTCCCGA
    GAAGCAGGAT TCAAACGCCT TCTTCAGTCA GGAGGAGCAA AGGTACTACC TGGTCATTCT
    GTACCTTTAT TTAAAGAAGC TACACATCTC TTTTCTGACT TCAATAAACT GAAACCAGAT
    GACTCAGGAG TTAATATAGC AGAAGCTGCT GCCCAAAATG TGTACTGCTT GAAAACAGAG
    TACATTGCTG ATTATCTGAT GCAGGAATCA CCTCCTCCAG TAGAAAATTA CTGTCTACCA
    GAAGCAGTTT CATTTCTTCA GAATAATAAG GAACCTGGGC CTGGATTATC ACAGAAGAGG
    AAAGCTCCTA CAGAGAAAAA TAAAATCAAA CGACCTAGAG TAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24161
    Clone ID Related Accession (Same CDS sequence) XM_534266.6 , XM_022408653.1 , XM_534266.5
    Accession Version XM_534266.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product DNA topoisomerase 2-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_534266.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTCTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    GAATGTTTTT TTAAAGCTCT GGAGTCAATA AAAGAATTTC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA CAGGAAAATG ATAGGTCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGC
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAACGATGTG TCCCAAGAGC TGAACATCCA
    GTCTATAATA TGGTTATGTC TGATGTAACT ATATCTTGTA CAAGCCTGGA AAAAGACAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GTGTATATAG AGATCTTAAT
    ATATCAGTAA CTCATCTTAT TGCAGGAGAA GTTGGCAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCGATTTT GCTTCCTTCT TGGATTAAAA CACTTTGGGA AAAGTCACAA
    GAGAAAAAAA TATCTAGATA TACTGATGTA AACATGGAAG ACTTCAAGTG CCCAATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGGTTG TGTAGCTTAG ATAGGAAGGC AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATATATG GGACAACTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT GTTGACAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ACCAGAAACA AAGACTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCC ACACAGTTGA CAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGTT GCATAAATGA ATCAATGTGT AATTCAGTGC TTAATAGCAA AGTGGAGCCT
    ACCCTTGAAA ATCTAGAAAA TTTGGATGTC AGTGCATTTC AAGCACCTGA AGATTTATTA
    GATGGTTGTC GGATCTATCT TTGCGGTTTT AGTGGCAGAA GGCTAGATAA ACTAAGAAGG
    CTTATTAACA GTGGAGGTGG AGTTCGTTTT AATCAGCTCA ATGAAGATGT AACTCATGTT
    ATAGTGGGAG ATTATGATGA TGAATTGAAG CAGTTTTGGG ATAAATCGGC CCACAGGCCT
    CATGTAGTAG GAGCAAAATG GTTGCTGGAG TGTTTTAGTA AAGGTTATAT GCTTCCTGAA
    GAAACATATA TCCATGCTAA CTACCAGCCA GTGGAAATTC CAGTTTCAGA TCAACCTGAA
    AATAAAACAG CCCTTTTAAA AAAGAACAAC AGCTTTTCTA AGAAAGACTT TGCTTCTGAT
    GAAAAGCATG AACAAGCTGA TGAAGATCTG CTCTCTCAAT ATGTAAATAA TAACCCGACA
    GTAGGTGAAG CTGTGAAGTC TGAAGTTGAG CCCTTTAATG ACTCTACTCA TATTTCTCCG
    CAAAAAGAAA ACCAGTCTTC TGCCACTCAT TGCCTCCCTG ATGCTTCTAC AACTACTGAA
    GAAGGTTTAT TTAGCCAAAA GAGTTTCCTT GTTTTGGGGT TTAGTAATGA AAATGAATCT
    AATATTGCAA ATATCATAAG AGAAAATGCT GGAAAAGTCG TGTCCCTTCA GAGCAGAATT
    GTTGCTGATT ATGCTGTGGT TCCTCTGCTG GGGTGTGAAG TAGAAGCAAC TGTAGGAGAA
    GTCGTCACAA ATACATGGCT GGTAACTTGT ATAGACTATC AGACTTTAAT TGATCCAAAG
    TCAAATCCTC TCTTCATGCC AGTTCCATTT ATGGCAGGAA TGACTCCTTT AGAGGATTGT
    GTTATTTCAT TCAGCCAGTG TGCAGGAGCA GAAAAAGATT CATTGACATT CTTAGCAAAT
    CGCCTTGGAG CTAGCGTTCA AGAATTCTTT GTTCGCAAAT CCAATGCAAA GAAAGGCATG
    TTTGCCAGTA CACATCTTGT ATTGAAAGAA CCAGGTGGTT CAAAATATGA AGCTGCAAAG
    AAGTGGAATT TGCCAGCAGT CACCATATCT TGGCTGTTGG AGACTGCTAG AATGGGAAAG
    AGAGCAAATG AAAACCATTT TCTGATTGAA AAGTCAACTA AAGAAGAACA AAGTTTGGAA
    ACTGAAATAA CAAACAAGGT GAATCTAAAT CCAGATACTC CAGAGCATCC TGTTGCACAC
    CTGGAAACTC AGAGGAAAAC AGCTGTTACA CCTTTAGATA TGAACCGCTT TCAGAGTAGA
    GCTTTCCATG CTGTGATCTC ACAACACACC AAACAGGTTT CGACCTCCTC GCCAGTGGGA
    CAGCCACTTC AGAAGGAGCC CTCCTTACAT CTGGACACAC CATCAAAGTT TCTGTCCAAG
    GACAAACTCT TCAAGCCTTC CTTTGATGTG AAGGATGCAC TTGCAGCCTT GGAAACTCCA
    GGAGGTCCCA GCCAGCAGAA AAGGAAACTG AGTACACCAC TTTCAGAAGT CATTGTCAGA
    AACTTGAAAC TTGCTTTAGC AAATAGCTCT CGAAATGCTG TTGCTCTTTC TGCCAGCCCT
    CGGCTGAAGG ATGCCCAATC AGAGAAGGAT GAAGCTCCAA AGCCACTTCA TAAGGTAGTG
    GTATGTGTGA GTAAGAAACT CAGTAAGAAG CAGAGTGAAC TGAATGGGAT CGCAGCCTCT
    TTAGGAGCAG ATTACAGATG GAGTTTTGAT GAAACCGTGA CTCATTTCAT TTATCAAGGC
    CGACCAAATG ACACTAACCG AGAGTATAAA TCTGTAAAAG AAAGAGGAGT ACACATTGTT
    TCAGAGCACT GGCTTTTAGA ATGTGCCCAA GAGTATAAAC ATCTTCCTGA ATCTCTTTAT
    CCACATACTT ATAATCCCAA AATGAGCTTG GATATCAGTA CAGTGCAAGA TGGCAGAATC
    TCTAATAGTC GACTGCTCTC AGCTGATTCA ACGACAAAGG ATGATGAGCC AGATCATTTG
    CCTGGAGAAG AAAACGATAT AGACAATATG ACCACCAGTA ATAAAGAATC CGCAACATCA
    AATGGAGATG GAAGGAATGA CTCTAAAGGA GCTCTGACAC AGACTTTGGA GATGAGAGAG
    AATTTTCAAA AGCAGCTGCA AGAGATAATG TCTGCAACAT CAATAGTGAA ACCTCAGGGA
    CCAAGGGCTT CCCTTTCAAG AAGTGGTTGT AACAGTGCTT CTTCAACCCC TGATAGCACT
    CGCTCTGCCC GCAGTGGGCG AAGTAGAGTC CTGGAGGCAC TGAGGCAGTC TCGTCAAACG
    GTACCTGATA TCAACACAGA GCCCTCCCAG AATGAACAGA TCATTTGGGA TGACCCGACA
    GCCAGGGAAG AGAGAGCAAG GCTTGCCAGC AATCTGCAGT GGCCTAGTTG TCCCACACAG
    TATTCTGAGC TTCAGGTTGA CATTAAAAAA TTGGAGGATT CTCCTTTCCA GGAGCCTTTA
    CATGATCCAG AAATTGCTGA ACAGGCAGTC TGTGATCCCG GAAATGTGTG TGTGACTGAA
    GCCCCAAAAC ACCCGACCTC TGAAGAACTG GAAACACCAG TAAAAGACAG CCACCTGATC
    CCTACACCTC AAGCTCCCAG TATTGCCTTC CCCCTAGCCA ACCCACCTGT GGCTCCACAC
    CCTAGAGAAA AGATTATAAC AATAGAGGAA ACCCATGAAG ACTTGAAAAA ACAGTACATA
    TTTCAGTTGT CGTCTCTGAA TCCTCAAGAA CGTATTGATT ATTGTCATCT GATTGAAAAA
    CTAGGTGGAT TAGTAATAGA GAAGCAATGC TTTGATCCCA ACTGTACACA CATTGTTGTG
    GGACATCCTC TTCGAAACGA GAAGTATTTA GCTTCAGTGG CAGCTGGGAA GTGGGTACTT
    CATCGCTCCT ACCTGGAAGC ATGCCGGACT GCTGGGCACT TCGTGCTGGA AGAAGACTAT
    GAATGGGGAA GTAGTTCCAT ACTTGATGTT TTGACTGGAA TCAATGTACA GCAACGAAGA
    CTAGCACTTG CAGCAATGAG ATGGAGGAAA AAAATCCAGC AAAGACAAGA ATCAGGCATT
    GTTGAGGGGG CATTTAGTGG GTGGAAGGTT ATCTTACATG TTGACCAGTC CCGAGAAGCA
    GGATTCAAAC GCCTTCTTCA GTCAGGAGGA GCAAAGGTAC TACCTGGTCA TTCTGTACCT
    TTATTTAAAG AAGCTACACA TCTCTTTTCT GACTTCAATA AACTGAAACC AGATGACTCA
    GGAGTTAATA TAGCAGAAGC TGCTGCCCAA AATGTGTACT GCTTGAAAAC AGAGTACATT
    GCTGATTATC TGATGCAGGA ATCACCTCCT CCAGTAGAAA ATTACTGTCT ACCAGAAGCA
    GTTTCATTTC TTCAGAATAA TAAGGAACCT GGGCCTGGAT TATCACAGAA GAGGAAAGCT
    CCTACAGAGA AAAATAAAAT CAAACGACCT AGAGTAAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_534266.3
    CDS144..4685
    Translation

    Target ORF information:

    RefSeq Version XM_534266.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_534266.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTCTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    GAATGTTTTT TTAAAGCTCT GGAGTCAATA AAAGAATTTC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA CAGGAAAATG ATAGGTCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGC
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAACGATGTG TCCCAAGAGC TGAACATCCA
    GTCTATAATA TGGTTATGTC TGATGTAACT ATATCTTGTA CAAGCCTGGA AAAAGACAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GTGTATATAG AGATCTTAAT
    ATATCAGTAA CTCATCTTAT TGCAGGAGAA GTTGGCAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCGATTTT GCTTCCTTCT TGGATTAAAA CACTTTGGGA AAAGTCACAA
    GAGAAAAAAA TATCTAGATA TACTGATGTA AACATGGAAG ACTTCAAGTG CCCAATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGGTTG TGTAGCTTAG ATAGGAAGGC AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATATATG GGACAACTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT GTTGACAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ACCAGAAACA AAGACTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCC ACACAGTTGA CAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGTT GCATAAATGA ATCAATGTGT AATTCAGTGC TTAATAGCAA AGTGGAGCCT
    ACCCTTGAAA ATCTAGAAAA TTTGGATGTC AGTGCATTTC AAGCACCTGA AGATTTATTA
    GATGGTTGTC GGATCTATCT TTGCGGTTTT AGTGGCAGAA GGCTAGATAA ACTAAGAAGG
    CTTATTAACA GTGGAGGTGG AGTTCGTTTT AATCAGCTCA ATGAAGATGT AACTCATGTT
    ATAGTGGGAG ATTATGATGA TGAATTGAAG CAGTTTTGGG ATAAATCGGC CCACAGGCCT
    CATGTAGTAG GAGCAAAATG GTTGCTGGAG TGTTTTAGTA AAGGTTATAT GCTTCCTGAA
    GAAACATATA TCCATGCTAA CTACCAGCCA GTGGAAATTC CAGTTTCAGA TCAACCTGAA
    AATAAAACAG CCCTTTTAAA AAAGAACAAC AGCTTTTCTA AGAAAGACTT TGCTTCTGAT
    GAAAAGCATG AACAAGCTGA TGAAGATCTG CTCTCTCAAT ATGTAAATAA TAACCCGACA
    GTAGGTGAAG CTGTGAAGTC TGAAGTTGAG CCCTTTAATG ACTCTACTCA TATTTCTCCG
    CAAAAAGAAA ACCAGTCTTC TGCCACTCAT TGCCTCCCTG ATGCTTCTAC AACTACTGAA
    GAAGGTTTAT TTAGCCAAAA GAGTTTCCTT GTTTTGGGGT TTAGTAATGA AAATGAATCT
    AATATTGCAA ATATCATAAG AGAAAATGCT GGAAAAGTCG TGTCCCTTCA GAGCAGAATT
    GTTGCTGATT ATGCTGTGGT TCCTCTGCTG GGGTGTGAAG TAGAAGCAAC TGTAGGAGAA
    GTCGTCACAA ATACATGGCT GGTAACTTGT ATAGACTATC AGACTTTAAT TGATCCAAAG
    TCAAATCCTC TCTTCATGCC AGTTCCATTT ATGGCAGGAA TGACTCCTTT AGAGGATTGT
    GTTATTTCAT TCAGCCAGTG TGCAGGAGCA GAAAAAGATT CATTGACATT CTTAGCAAAT
    CGCCTTGGAG CTAGCGTTCA AGAATTCTTT GTTCGCAAAT CCAATGCAAA GAAAGGCATG
    TTTGCCAGTA CACATCTTGT ATTGAAAGAA CCAGGTGGTT CAAAATATGA AGCTGCAAAG
    AAGTGGAATT TGCCAGCAGT CACCATATCT TGGCTGTTGG AGACTGCTAG AATGGGAAAG
    AGAGCAAATG AAAACCATTT TCTGATTGAA AAGTCAACTA AAGAAGAACA AAGTTTGGAA
    ACTGAAATAA CAAACAAGGT GAATCTAAAT CCAGATACTC CAGAGCATCC TGTTGCACAC
    CTGGAAACTC AGAGGAAAAC AGCTGTTACA CCTTTAGATA TGAACCGCTT TCAGAGTAGA
    GCTTTCCATG CTGTGATCTC ACAACACACC AAACAGGTTT CGACCTCCTC GCCAGTGGGA
    CAGCCACTTC AGAAGGAGCC CTCCTTACAT CTGGACACAC CATCAAAGTT TCTGTCCAAG
    GACAAACTCT TCAAGCCTTC CTTTGATGTG AAGGATGCAC TTGCAGCCTT GGAAACTCCA
    GGAGGTCCCA GCCAGCAGAA AAGGAAACTG AGTACACCAC TTTCAGAAGT CATTGTCAGA
    AACTTGAAAC TTGCTTTAGC AAATAGCTCT CGAAATGCTG TTGCTCTTTC TGCCAGCCCT
    CGGCTGAAGG ATGCCCAATC AGAGAAGGAT GAAGCTCCAA AGCCACTTCA TAAGGTAGTG
    GTATGTGTGA GTAAGAAACT CAGTAAGAAG CAGAGTGAAC TGAATGGGAT CGCAGCCTCT
    TTAGGAGCAG ATTACAGATG GAGTTTTGAT GAAACCGTGA CTCATTTCAT TTATCAAGGC
    CGACCAAATG ACACTAACCG AGAGTATAAA TCTGTAAAAG AAAGAGGAGT ACACATTGTT
    TCAGAGCACT GGCTTTTAGA ATGTGCCCAA GAGTATAAAC ATCTTCCTGA ATCTCTTTAT
    CCACATACTT ATAATCCCAA AATGAGCTTG GATATCAGTA CAGTGCAAGA TGGCAGAATC
    TCTAATAGTC GACTGCTCTC AGCTGATTCA ACGACAAAGG ATGATGAGCC AGATCATTTG
    CCTGGAGAAG AAAACGATAT AGACAATATG ACCACCAGTA ATAAAGAATC CGCAACATCA
    AATGGAGATG GAAGGAATGA CTCTAAAGGA GCTCTGACAC AGACTTTGGA GATGAGAGAG
    AATTTTCAAA AGCAGCTGCA AGAGATAATG TCTGCAACAT CAATAGTGAA ACCTCAGGGA
    CCAAGGGCTT CCCTTTCAAG AAGTGGTTGT AACAGTGCTT CTTCAACCCC TGATAGCACT
    CGCTCTGCCC GCAGTGGGCG AAGTAGAGTC CTGGAGGCAC TGAGGCAGTC TCGTCAAACG
    GTACCTGATA TCAACACAGA GCCCTCCCAG AATGAACAGA TCATTTGGGA TGACCCGACA
    GCCAGGGAAG AGAGAGCAAG GCTTGCCAGC AATCTGCAGT GGCCTAGTTG TCCCACACAG
    TATTCTGAGC TTCAGGTTGA CATTAAAAAA TTGGAGGATT CTCCTTTCCA GGAGCCTTTA
    CATGATCCAG AAATTGCTGA ACAGGCAGTC TGTGATCCCG GAAATGTGTG TGTGACTGAA
    GCCCCAAAAC ACCCGACCTC TGAAGAACTG GAAACACCAG TAAAAGACAG CCACCTGATC
    CCTACACCTC AAGCTCCCAG TATTGCCTTC CCCCTAGCCA ACCCACCTGT GGCTCCACAC
    CCTAGAGAAA AGATTATAAC AATAGAGGAA ACCCATGAAG ACTTGAAAAA ACAGTACATA
    TTTCAGTTGT CGTCTCTGAA TCCTCAAGAA CGTATTGATT ATTGTCATCT GATTGAAAAA
    CTAGGTGGAT TAGTAATAGA GAAGCAATGC TTTGATCCCA ACTGTACACA CATTGTTGTG
    GGACATCCTC TTCGAAACGA GAAGTATTTA GCTTCAGTGG CAGCTGGGAA GTGGGTACTT
    CATCGCTCCT ACCTGGAAGC ATGCCGGACT GCTGGGCACT TCGTGCTGGA AGAAGACTAT
    GAATGGGGAA GTAGTTCCAT ACTTGATGTT TTGACTGGAA TCAATGTACA GCAACGAAGA
    CTAGCACTTG CAGCAATGAG ATGGAGGAAA AAAATCCAGC AAAGACAAGA ATCAGGCATT
    GTTGAGGGGG CATTTAGTGG GTGGAAGGTT ATCTTACATG TTGACCAGTC CCGAGAAGCA
    GGATTCAAAC GCCTTCTTCA GTCAGGAGGA GCAAAGGTAC TACCTGGTCA TTCTGTACCT
    TTATTTAAAG AAGCTACACA TCTCTTTTCT GACTTCAATA AACTGAAACC AGATGACTCA
    GGAGTTAATA TAGCAGAAGC TGCTGCCCAA AATGTGTACT GCTTGAAAAC AGAGTACATT
    GCTGATTATC TGATGCAGGA ATCACCTCCT CCAGTAGAAA ATTACTGTCT ACCAGAAGCA
    GTTTCATTTC TTCAGAATAA TAAGGAACCT GGGCCTGGAT TATCACAGAA GAGGAAAGCT
    CCTACAGAGA AAAATAAAAT CAAACGACCT AGAGTAAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24161
    Clone ID Related Accession (Same CDS sequence) XM_534266.6 , XM_022408653.1 , XM_534266.5
    Accession Version XM_022408653.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product DNA topoisomerase 2-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTCTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    GAATGTTTTT TTAAAGCTCT GGAGTCAATA AAAGAATTTC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA CAGGAAAATG ATAGGTCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGC
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAACGATGTG TCCCAAGAGC TGAACATCCA
    GTCTATAATA TGGTTATGTC TGATGTAACT ATATCTTGTA CAAGCCTGGA AAAAGACAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GTGTATATAG AGATCTTAAT
    ATATCAGTAA CTCATCTTAT TGCAGGAGAA GTTGGCAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCGATTTT GCTTCCTTCT TGGATTAAAA CACTTTGGGA AAAGTCACAA
    GAGAAAAAAA TATCTAGATA TACTGATGTA AACATGGAAG ACTTCAAGTG CCCAATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGGTTG TGTAGCTTAG ATAGGAAGGC AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATATATG GGACAACTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT GTTGACAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ACCAGAAACA AAGACTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCC ACACAGTTGA CAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGTT GCATAAATGA ATCAATGTGT AATTCAGTGC TTAATAGCAA AGTGGAGCCT
    ACCCTTGAAA ATCTAGAAAA TTTGGATGTC AGTGCATTTC AAGCACCTGA AGATTTATTA
    GATGGTTGTC GGATCTATCT TTGCGGTTTT AGTGGCAGAA GGCTAGATAA ACTAAGAAGG
    CTTATTAACA GTGGAGGTGG AGTTCGTTTT AATCAGCTCA ATGAAGATGT AACTCATGTT
    ATAGTGGGAG ATTATGATGA TGAATTGAAG CAGTTTTGGG ATAAATCGGC CCACAGGCCT
    CATGTAGTAG GAGCAAAATG GTTGCTGGAG TGTTTTAGTA AAGGTTATAT GCTTCCTGAA
    GAAACATATA TCCATGCTAA CTACCAGCCA GTGGAAATTC CAGTTTCAGA TCAACCTGAA
    AATAAAACAG CCCTTTTAAA AAAGAACAAC AGCTTTTCTA AGAAAGACTT TGCTTCTGAT
    GAAAAGCATG AACAAGCTGA TGAAGATCTG CTCTCTCAAT ATGTAAATAA TAACCCGACA
    GTAGGTGAAG CTGTGAAGTC TGAAGTTGAG CCCTTTAATG ACTCTACTCA TATTTCTCCG
    CAAAAAGAAA ACCAGTCTTC TGCCACTCAT TGCCTCCCTG ATGCTTCTAC AACTACTGAA
    GAAGGTTTAT TTAGCCAAAA GAGTTTCCTT GTTTTGGGGT TTAGTAATGA AAATGAATCT
    AATATTGCAA ATATCATAAG AGAAAATGCT GGAAAAGTCG TGTCCCTTCA GAGCAGAATT
    GTTGCTGATT ATGCTGTGGT TCCTCTGCTG GGGTGTGAAG TAGAAGCAAC TGTAGGAGAA
    GTCGTCACAA ATACATGGCT GGTAACTTGT ATAGACTATC AGACTTTAAT TGATCCAAAG
    TCAAATCCTC TCTTCATGCC AGTTCCATTT ATGGCAGGAA TGACTCCTTT AGAGGATTGT
    GTTATTTCAT TCAGCCAGTG TGCAGGAGCA GAAAAAGATT CATTGACATT CTTAGCAAAT
    CGCCTTGGAG CTAGCGTTCA AGAATTCTTT GTTCGCAAAT CCAATGCAAA GAAAGGCATG
    TTTGCCAGTA CACATCTTGT ATTGAAAGAA CCAGGTGGTT CAAAATATGA AGCTGCAAAG
    AAGTGGAATT TGCCAGCAGT CACCATATCT TGGCTGTTGG AGACTGCTAG AATGGGAAAG
    AGAGCAAATG AAAACCATTT TCTGATTGAA AAGTCAACTA AAGAAGAACA AAGTTTGGAA
    ACTGAAATAA CAAACAAGGT GAATCTAAAT CCAGATACTC CAGAGCATCC TGTTGCACAC
    CTGGAAACTC AGAGGAAAAC AGCTGTTACA CCTTTAGATA TGAACCGCTT TCAGAGTAGA
    GCTTTCCATG CTGTGATCTC ACAACACACC AAACAGGTTT CGACCTCCTC GCCAGTGGGA
    CAGCCACTTC AGAAGGAGCC CTCCTTACAT CTGGACACAC CATCAAAGTT TCTGTCCAAG
    GACAAACTCT TCAAGCCTTC CTTTGATGTG AAGGATGCAC TTGCAGCCTT GGAAACTCCA
    GGAGGTCCCA GCCAGCAGAA AAGGAAACTG AGTACACCAC TTTCAGAAGT CATTGTCAGA
    AACTTGAAAC TTGCTTTAGC AAATAGCTCT CGAAATGCTG TTGCTCTTTC TGCCAGCCCT
    CGGCTGAAGG ATGCCCAATC AGAGAAGGAT GAAGCTCCAA AGCCACTTCA TAAGGTAGTG
    GTATGTGTGA GTAAGAAACT CAGTAAGAAG CAGAGTGAAC TGAATGGGAT CGCAGCCTCT
    TTAGGAGCAG ATTACAGATG GAGTTTTGAT GAAACCGTGA CTCATTTCAT TTATCAAGGC
    CGACCAAATG ACACTAACCG AGAGTATAAA TCTGTAAAAG AAAGAGGAGT ACACATTGTT
    TCAGAGCACT GGCTTTTAGA ATGTGCCCAA GAGTATAAAC ATCTTCCTGA ATCTCTTTAT
    CCACATACTT ATAATCCCAA AATGAGCTTG GATATCAGTA CAGTGCAAGA TGGCAGAATC
    TCTAATAGTC GACTGCTCTC AGCTGATTCA ACGACAAAGG ATGATGAGCC AGATCATTTG
    CCTGGAGAAG AAAACGATAT AGACAATATG ACCACCAGTA ATAAAGAATC CGCAACATCA
    AATGGAGATG GAAGGAATGA CTCTAAAGGA GCTCTGACAC AGACTTTGGA GATGAGAGAG
    AATTTTCAAA AGCAGCTGCA AGAGATAATG TCTGCAACAT CAATAGTGAA ACCTCAGGGA
    CCAAGGGCTT CCCTTTCAAG AAGTGGTTGT AACAGTGCTT CTTCAACCCC TGATAGCACT
    CGCTCTGCCC GCAGTGGGCG AAGTAGAGTC CTGGAGGCAC TGAGGCAGTC TCGTCAAACG
    GTACCTGATA TCAACACAGA GCCCTCCCAG AATGAACAGA TCATTTGGGA TGACCCGACA
    GCCAGGGAAG AGAGAGCAAG GCTTGCCAGC AATCTGCAGT GGCCTAGTTG TCCCACACAG
    TATTCTGAGC TTCAGGTTGA CATTAAAAAA TTGGAGGATT CTCCTTTCCA GGAGCCTTTA
    CATGATCCAG AAATTGCTGA ACAGGCAGTC TGTGATCCCG GAAATGTGTG TGTGACTGAA
    GCCCCAAAAC ACCCGACCTC TGAAGAACTG GAAACACCAG TAAAAGACAG CCACCTGATC
    CCTACACCTC AAGCTCCCAG TATTGCCTTC CCCCTAGCCA ACCCACCTGT GGCTCCACAC
    CCTAGAGAAA AGATTATAAC AATAGAGGAA ACCCATGAAG ACTTGAAAAA ACAGTACATA
    TTTCAGTTGT CGTCTCTGAA TCCTCAAGAA CGTATTGATT ATTGTCATCT GATTGAAAAA
    CTAGGTGGAT TAGTAATAGA GAAGCAATGC TTTGATCCCA ACTGTACACA CATTGTTGTG
    GGACATCCTC TTCGAAACGA GAAGTATTTA GCTTCAGTGG CAGCTGGGAA GTGGGTACTT
    CATCGCTCCT ACCTGGAAGC ATGCCGGACT GCTGGGCACT TCGTGCTGGA AGAAGACTAT
    GAATGGGGAA GTAGTTCCAT ACTTGATGTT TTGACTGGAA TCAATGTACA GCAACGAAGA
    CTAGCACTTG CAGCAATGAG ATGGAGGAAA AAAATCCAGC AAAGACAAGA ATCAGGCATT
    GTTGAGGGGG CATTTAGTGG GTGGAAGGTT ATCTTACATG TTGACCAGTC CCGAGAAGCA
    GGATTCAAAC GCCTTCTTCA GTCAGGAGGA GCAAAGGTAC TACCTGGTCA TTCTGTACCT
    TTATTTAAAG AAGCTACACA TCTCTTTTCT GACTTCAATA AACTGAAACC AGATGACTCA
    GGAGTTAATA TAGCAGAAGC TGCTGCCCAA AATGTGTACT GCTTGAAAAC AGAGTACATT
    GCTGATTATC TGATGCAGGA ATCACCTCCT CCAGTAGAAA ATTACTGTCT ACCAGAAGCA
    GTTTCATTTC TTCAGAATAA TAAGGAACCT GGGCCTGGAT TATCACAGAA GAGGAAAGCT
    CCTACAGAGA AAAATAAAAT CAAACGACCT AGAGTAAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022264361.1
    CDS303..4844
    Translation

    Target ORF information:

    RefSeq Version XM_022408653.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022408653.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTCTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    GAATGTTTTT TTAAAGCTCT GGAGTCAATA AAAGAATTTC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA CAGGAAAATG ATAGGTCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGC
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAACGATGTG TCCCAAGAGC TGAACATCCA
    GTCTATAATA TGGTTATGTC TGATGTAACT ATATCTTGTA CAAGCCTGGA AAAAGACAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GTGTATATAG AGATCTTAAT
    ATATCAGTAA CTCATCTTAT TGCAGGAGAA GTTGGCAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCGATTTT GCTTCCTTCT TGGATTAAAA CACTTTGGGA AAAGTCACAA
    GAGAAAAAAA TATCTAGATA TACTGATGTA AACATGGAAG ACTTCAAGTG CCCAATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGGTTG TGTAGCTTAG ATAGGAAGGC AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATATATG GGACAACTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT GTTGACAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ACCAGAAACA AAGACTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCC ACACAGTTGA CAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGTT GCATAAATGA ATCAATGTGT AATTCAGTGC TTAATAGCAA AGTGGAGCCT
    ACCCTTGAAA ATCTAGAAAA TTTGGATGTC AGTGCATTTC AAGCACCTGA AGATTTATTA
    GATGGTTGTC GGATCTATCT TTGCGGTTTT AGTGGCAGAA GGCTAGATAA ACTAAGAAGG
    CTTATTAACA GTGGAGGTGG AGTTCGTTTT AATCAGCTCA ATGAAGATGT AACTCATGTT
    ATAGTGGGAG ATTATGATGA TGAATTGAAG CAGTTTTGGG ATAAATCGGC CCACAGGCCT
    CATGTAGTAG GAGCAAAATG GTTGCTGGAG TGTTTTAGTA AAGGTTATAT GCTTCCTGAA
    GAAACATATA TCCATGCTAA CTACCAGCCA GTGGAAATTC CAGTTTCAGA TCAACCTGAA
    AATAAAACAG CCCTTTTAAA AAAGAACAAC AGCTTTTCTA AGAAAGACTT TGCTTCTGAT
    GAAAAGCATG AACAAGCTGA TGAAGATCTG CTCTCTCAAT ATGTAAATAA TAACCCGACA
    GTAGGTGAAG CTGTGAAGTC TGAAGTTGAG CCCTTTAATG ACTCTACTCA TATTTCTCCG
    CAAAAAGAAA ACCAGTCTTC TGCCACTCAT TGCCTCCCTG ATGCTTCTAC AACTACTGAA
    GAAGGTTTAT TTAGCCAAAA GAGTTTCCTT GTTTTGGGGT TTAGTAATGA AAATGAATCT
    AATATTGCAA ATATCATAAG AGAAAATGCT GGAAAAGTCG TGTCCCTTCA GAGCAGAATT
    GTTGCTGATT ATGCTGTGGT TCCTCTGCTG GGGTGTGAAG TAGAAGCAAC TGTAGGAGAA
    GTCGTCACAA ATACATGGCT GGTAACTTGT ATAGACTATC AGACTTTAAT TGATCCAAAG
    TCAAATCCTC TCTTCATGCC AGTTCCATTT ATGGCAGGAA TGACTCCTTT AGAGGATTGT
    GTTATTTCAT TCAGCCAGTG TGCAGGAGCA GAAAAAGATT CATTGACATT CTTAGCAAAT
    CGCCTTGGAG CTAGCGTTCA AGAATTCTTT GTTCGCAAAT CCAATGCAAA GAAAGGCATG
    TTTGCCAGTA CACATCTTGT ATTGAAAGAA CCAGGTGGTT CAAAATATGA AGCTGCAAAG
    AAGTGGAATT TGCCAGCAGT CACCATATCT TGGCTGTTGG AGACTGCTAG AATGGGAAAG
    AGAGCAAATG AAAACCATTT TCTGATTGAA AAGTCAACTA AAGAAGAACA AAGTTTGGAA
    ACTGAAATAA CAAACAAGGT GAATCTAAAT CCAGATACTC CAGAGCATCC TGTTGCACAC
    CTGGAAACTC AGAGGAAAAC AGCTGTTACA CCTTTAGATA TGAACCGCTT TCAGAGTAGA
    GCTTTCCATG CTGTGATCTC ACAACACACC AAACAGGTTT CGACCTCCTC GCCAGTGGGA
    CAGCCACTTC AGAAGGAGCC CTCCTTACAT CTGGACACAC CATCAAAGTT TCTGTCCAAG
    GACAAACTCT TCAAGCCTTC CTTTGATGTG AAGGATGCAC TTGCAGCCTT GGAAACTCCA
    GGAGGTCCCA GCCAGCAGAA AAGGAAACTG AGTACACCAC TTTCAGAAGT CATTGTCAGA
    AACTTGAAAC TTGCTTTAGC AAATAGCTCT CGAAATGCTG TTGCTCTTTC TGCCAGCCCT
    CGGCTGAAGG ATGCCCAATC AGAGAAGGAT GAAGCTCCAA AGCCACTTCA TAAGGTAGTG
    GTATGTGTGA GTAAGAAACT CAGTAAGAAG CAGAGTGAAC TGAATGGGAT CGCAGCCTCT
    TTAGGAGCAG ATTACAGATG GAGTTTTGAT GAAACCGTGA CTCATTTCAT TTATCAAGGC
    CGACCAAATG ACACTAACCG AGAGTATAAA TCTGTAAAAG AAAGAGGAGT ACACATTGTT
    TCAGAGCACT GGCTTTTAGA ATGTGCCCAA GAGTATAAAC ATCTTCCTGA ATCTCTTTAT
    CCACATACTT ATAATCCCAA AATGAGCTTG GATATCAGTA CAGTGCAAGA TGGCAGAATC
    TCTAATAGTC GACTGCTCTC AGCTGATTCA ACGACAAAGG ATGATGAGCC AGATCATTTG
    CCTGGAGAAG AAAACGATAT AGACAATATG ACCACCAGTA ATAAAGAATC CGCAACATCA
    AATGGAGATG GAAGGAATGA CTCTAAAGGA GCTCTGACAC AGACTTTGGA GATGAGAGAG
    AATTTTCAAA AGCAGCTGCA AGAGATAATG TCTGCAACAT CAATAGTGAA ACCTCAGGGA
    CCAAGGGCTT CCCTTTCAAG AAGTGGTTGT AACAGTGCTT CTTCAACCCC TGATAGCACT
    CGCTCTGCCC GCAGTGGGCG AAGTAGAGTC CTGGAGGCAC TGAGGCAGTC TCGTCAAACG
    GTACCTGATA TCAACACAGA GCCCTCCCAG AATGAACAGA TCATTTGGGA TGACCCGACA
    GCCAGGGAAG AGAGAGCAAG GCTTGCCAGC AATCTGCAGT GGCCTAGTTG TCCCACACAG
    TATTCTGAGC TTCAGGTTGA CATTAAAAAA TTGGAGGATT CTCCTTTCCA GGAGCCTTTA
    CATGATCCAG AAATTGCTGA ACAGGCAGTC TGTGATCCCG GAAATGTGTG TGTGACTGAA
    GCCCCAAAAC ACCCGACCTC TGAAGAACTG GAAACACCAG TAAAAGACAG CCACCTGATC
    CCTACACCTC AAGCTCCCAG TATTGCCTTC CCCCTAGCCA ACCCACCTGT GGCTCCACAC
    CCTAGAGAAA AGATTATAAC AATAGAGGAA ACCCATGAAG ACTTGAAAAA ACAGTACATA
    TTTCAGTTGT CGTCTCTGAA TCCTCAAGAA CGTATTGATT ATTGTCATCT GATTGAAAAA
    CTAGGTGGAT TAGTAATAGA GAAGCAATGC TTTGATCCCA ACTGTACACA CATTGTTGTG
    GGACATCCTC TTCGAAACGA GAAGTATTTA GCTTCAGTGG CAGCTGGGAA GTGGGTACTT
    CATCGCTCCT ACCTGGAAGC ATGCCGGACT GCTGGGCACT TCGTGCTGGA AGAAGACTAT
    GAATGGGGAA GTAGTTCCAT ACTTGATGTT TTGACTGGAA TCAATGTACA GCAACGAAGA
    CTAGCACTTG CAGCAATGAG ATGGAGGAAA AAAATCCAGC AAAGACAAGA ATCAGGCATT
    GTTGAGGGGG CATTTAGTGG GTGGAAGGTT ATCTTACATG TTGACCAGTC CCGAGAAGCA
    GGATTCAAAC GCCTTCTTCA GTCAGGAGGA GCAAAGGTAC TACCTGGTCA TTCTGTACCT
    TTATTTAAAG AAGCTACACA TCTCTTTTCT GACTTCAATA AACTGAAACC AGATGACTCA
    GGAGTTAATA TAGCAGAAGC TGCTGCCCAA AATGTGTACT GCTTGAAAAC AGAGTACATT
    GCTGATTATC TGATGCAGGA ATCACCTCCT CCAGTAGAAA ATTACTGTCT ACCAGAAGCA
    GTTTCATTTC TTCAGAATAA TAAGGAACCT GGGCCTGGAT TATCACAGAA GAGGAAAGCT
    CCTACAGAGA AAAATAAAAT CAAACGACCT AGAGTAAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24161
    Clone ID Related Accession (Same CDS sequence) XM_534266.6 , XM_022408653.1 , XM_534266.5
    Accession Version XM_534266.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DNA topoisomerase 2-binding protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_534266.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTCTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    GAATGTTTTT TTAAAGCTCT GGAGTCAATA AAAGAATTTC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA CAGGAAAATG ATAGGTCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGC
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAACGATGTG TCCCAAGAGC TGAACATCCA
    GTCTATAATA TGGTTATGTC TGATGTAACT ATATCTTGTA CAAGCCTGGA AAAAGACAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GTGTATATAG AGATCTTAAT
    ATATCAGTAA CTCATCTTAT TGCAGGAGAA GTTGGCAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCGATTTT GCTTCCTTCT TGGATTAAAA CACTTTGGGA AAAGTCACAA
    GAGAAAAAAA TATCTAGATA TACTGATGTA AACATGGAAG ACTTCAAGTG CCCAATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGGTTG TGTAGCTTAG ATAGGAAGGC AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATATATG GGACAACTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT GTTGACAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ACCAGAAACA AAGACTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCC ACACAGTTGA CAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGTT GCATAAATGA ATCAATGTGT AATTCAGTGC TTAATAGCAA AGTGGAGCCT
    ACCCTTGAAA ATCTAGAAAA TTTGGATGTC AGTGCATTTC AAGCACCTGA AGATTTATTA
    GATGGTTGTC GGATCTATCT TTGCGGTTTT AGTGGCAGAA GGCTAGATAA ACTAAGAAGG
    CTTATTAACA GTGGAGGTGG AGTTCGTTTT AATCAGCTCA ATGAAGATGT AACTCATGTT
    ATAGTGGGAG ATTATGATGA TGAATTGAAG CAGTTTTGGG ATAAATCGGC CCACAGGCCT
    CATGTAGTAG GAGCAAAATG GTTGCTGGAG TGTTTTAGTA AAGGTTATAT GCTTCCTGAA
    GAAACATATA TCCATGCTAA CTACCAGCCA GTGGAAATTC CAGTTTCAGA TCAACCTGAA
    AATAAAACAG CCCTTTTAAA AAAGAACAAC AGCTTTTCTA AGAAAGACTT TGCTTCTGAT
    GAAAAGCATG AACAAGCTGA TGAAGATCTG CTCTCTCAAT ATGTAAATAA TAACCCGACA
    GTAGGTGAAG CTGTGAAGTC TGAAGTTGAG CCCTTTAATG ACTCTACTCA TATTTCTCCG
    CAAAAAGAAA ACCAGTCTTC TGCCACTCAT TGCCTCCCTG ATGCTTCTAC AACTACTGAA
    GAAGGTTTAT TTAGCCAAAA GAGTTTCCTT GTTTTGGGGT TTAGTAATGA AAATGAATCT
    AATATTGCAA ATATCATAAG AGAAAATGCT GGAAAAGTCG TGTCCCTTCA GAGCAGAATT
    GTTGCTGATT ATGCTGTGGT TCCTCTGCTG GGGTGTGAAG TAGAAGCAAC TGTAGGAGAA
    GTCGTCACAA ATACATGGCT GGTAACTTGT ATAGACTATC AGACTTTAAT TGATCCAAAG
    TCAAATCCTC TCTTCATGCC AGTTCCATTT ATGGCAGGAA TGACTCCTTT AGAGGATTGT
    GTTATTTCAT TCAGCCAGTG TGCAGGAGCA GAAAAAGATT CATTGACATT CTTAGCAAAT
    CGCCTTGGAG CTAGCGTTCA AGAATTCTTT GTTCGCAAAT CCAATGCAAA GAAAGGCATG
    TTTGCCAGTA CACATCTTGT ATTGAAAGAA CCAGGTGGTT CAAAATATGA AGCTGCAAAG
    AAGTGGAATT TGCCAGCAGT CACCATATCT TGGCTGTTGG AGACTGCTAG AATGGGAAAG
    AGAGCAAATG AAAACCATTT TCTGATTGAA AAGTCAACTA AAGAAGAACA AAGTTTGGAA
    ACTGAAATAA CAAACAAGGT GAATCTAAAT CCAGATACTC CAGAGCATCC TGTTGCACAC
    CTGGAAACTC AGAGGAAAAC AGCTGTTACA CCTTTAGATA TGAACCGCTT TCAGAGTAGA
    GCTTTCCATG CTGTGATCTC ACAACACACC AAACAGGTTT CGACCTCCTC GCCAGTGGGA
    CAGCCACTTC AGAAGGAGCC CTCCTTACAT CTGGACACAC CATCAAAGTT TCTGTCCAAG
    GACAAACTCT TCAAGCCTTC CTTTGATGTG AAGGATGCAC TTGCAGCCTT GGAAACTCCA
    GGAGGTCCCA GCCAGCAGAA AAGGAAACTG AGTACACCAC TTTCAGAAGT CATTGTCAGA
    AACTTGAAAC TTGCTTTAGC AAATAGCTCT CGAAATGCTG TTGCTCTTTC TGCCAGCCCT
    CGGCTGAAGG ATGCCCAATC AGAGAAGGAT GAAGCTCCAA AGCCACTTCA TAAGGTAGTG
    GTATGTGTGA GTAAGAAACT CAGTAAGAAG CAGAGTGAAC TGAATGGGAT CGCAGCCTCT
    TTAGGAGCAG ATTACAGATG GAGTTTTGAT GAAACCGTGA CTCATTTCAT TTATCAAGGC
    CGACCAAATG ACACTAACCG AGAGTATAAA TCTGTAAAAG AAAGAGGAGT ACACATTGTT
    TCAGAGCACT GGCTTTTAGA ATGTGCCCAA GAGTATAAAC ATCTTCCTGA ATCTCTTTAT
    CCACATACTT ATAATCCCAA AATGAGCTTG GATATCAGTA CAGTGCAAGA TGGCAGAATC
    TCTAATAGTC GACTGCTCTC AGCTGATTCA ACGACAAAGG ATGATGAGCC AGATCATTTG
    CCTGGAGAAG AAAACGATAT AGACAATATG ACCACCAGTA ATAAAGAATC CGCAACATCA
    AATGGAGATG GAAGGAATGA CTCTAAAGGA GCTCTGACAC AGACTTTGGA GATGAGAGAG
    AATTTTCAAA AGCAGCTGCA AGAGATAATG TCTGCAACAT CAATAGTGAA ACCTCAGGGA
    CCAAGGGCTT CCCTTTCAAG AAGTGGTTGT AACAGTGCTT CTTCAACCCC TGATAGCACT
    CGCTCTGCCC GCAGTGGGCG AAGTAGAGTC CTGGAGGCAC TGAGGCAGTC TCGTCAAACG
    GTACCTGATA TCAACACAGA GCCCTCCCAG AATGAACAGA TCATTTGGGA TGACCCGACA
    GCCAGGGAAG AGAGAGCAAG GCTTGCCAGC AATCTGCAGT GGCCTAGTTG TCCCACACAG
    TATTCTGAGC TTCAGGTTGA CATTAAAAAA TTGGAGGATT CTCCTTTCCA GGAGCCTTTA
    CATGATCCAG AAATTGCTGA ACAGGCAGTC TGTGATCCCG GAAATGTGTG TGTGACTGAA
    GCCCCAAAAC ACCCGACCTC TGAAGAACTG GAAACACCAG TAAAAGACAG CCACCTGATC
    CCTACACCTC AAGCTCCCAG TATTGCCTTC CCCCTAGCCA ACCCACCTGT GGCTCCACAC
    CCTAGAGAAA AGATTATAAC AATAGAGGAA ACCCATGAAG ACTTGAAAAA ACAGTACATA
    TTTCAGTTGT CGTCTCTGAA TCCTCAAGAA CGTATTGATT ATTGTCATCT GATTGAAAAA
    CTAGGTGGAT TAGTAATAGA GAAGCAATGC TTTGATCCCA ACTGTACACA CATTGTTGTG
    GGACATCCTC TTCGAAACGA GAAGTATTTA GCTTCAGTGG CAGCTGGGAA GTGGGTACTT
    CATCGCTCCT ACCTGGAAGC ATGCCGGACT GCTGGGCACT TCGTGCTGGA AGAAGACTAT
    GAATGGGGAA GTAGTTCCAT ACTTGATGTT TTGACTGGAA TCAATGTACA GCAACGAAGA
    CTAGCACTTG CAGCAATGAG ATGGAGGAAA AAAATCCAGC AAAGACAAGA ATCAGGCATT
    GTTGAGGGGG CATTTAGTGG GTGGAAGGTT ATCTTACATG TTGACCAGTC CCGAGAAGCA
    GGATTCAAAC GCCTTCTTCA GTCAGGAGGA GCAAAGGTAC TACCTGGTCA TTCTGTACCT
    TTATTTAAAG AAGCTACACA TCTCTTTTCT GACTTCAATA AACTGAAACC AGATGACTCA
    GGAGTTAATA TAGCAGAAGC TGCTGCCCAA AATGTGTACT GCTTGAAAAC AGAGTACATT
    GCTGATTATC TGATGCAGGA ATCACCTCCT CCAGTAGAAA ATTACTGTCT ACCAGAAGCA
    GTTTCATTTC TTCAGAATAA TAAGGAACCT GGGCCTGGAT TATCACAGAA GAGGAAAGCT
    CCTACAGAGA AAAATAAAAT CAAACGACCT AGAGTAAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_534266.3
    CDS184..4725
    Translation

    Target ORF information:

    RefSeq Version XM_534266.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_534266.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTCTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    GAATGTTTTT TTAAAGCTCT GGAGTCAATA AAAGAATTTC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA CAGGAAAATG ATAGGTCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGC
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAACGATGTG TCCCAAGAGC TGAACATCCA
    GTCTATAATA TGGTTATGTC TGATGTAACT ATATCTTGTA CAAGCCTGGA AAAAGACAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GTGTATATAG AGATCTTAAT
    ATATCAGTAA CTCATCTTAT TGCAGGAGAA GTTGGCAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCGATTTT GCTTCCTTCT TGGATTAAAA CACTTTGGGA AAAGTCACAA
    GAGAAAAAAA TATCTAGATA TACTGATGTA AACATGGAAG ACTTCAAGTG CCCAATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGGTTG TGTAGCTTAG ATAGGAAGGC AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATATATG GGACAACTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT GTTGACAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ACCAGAAACA AAGACTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCC ACACAGTTGA CAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGTT GCATAAATGA ATCAATGTGT AATTCAGTGC TTAATAGCAA AGTGGAGCCT
    ACCCTTGAAA ATCTAGAAAA TTTGGATGTC AGTGCATTTC AAGCACCTGA AGATTTATTA
    GATGGTTGTC GGATCTATCT TTGCGGTTTT AGTGGCAGAA GGCTAGATAA ACTAAGAAGG
    CTTATTAACA GTGGAGGTGG AGTTCGTTTT AATCAGCTCA ATGAAGATGT AACTCATGTT
    ATAGTGGGAG ATTATGATGA TGAATTGAAG CAGTTTTGGG ATAAATCGGC CCACAGGCCT
    CATGTAGTAG GAGCAAAATG GTTGCTGGAG TGTTTTAGTA AAGGTTATAT GCTTCCTGAA
    GAAACATATA TCCATGCTAA CTACCAGCCA GTGGAAATTC CAGTTTCAGA TCAACCTGAA
    AATAAAACAG CCCTTTTAAA AAAGAACAAC AGCTTTTCTA AGAAAGACTT TGCTTCTGAT
    GAAAAGCATG AACAAGCTGA TGAAGATCTG CTCTCTCAAT ATGTAAATAA TAACCCGACA
    GTAGGTGAAG CTGTGAAGTC TGAAGTTGAG CCCTTTAATG ACTCTACTCA TATTTCTCCG
    CAAAAAGAAA ACCAGTCTTC TGCCACTCAT TGCCTCCCTG ATGCTTCTAC AACTACTGAA
    GAAGGTTTAT TTAGCCAAAA GAGTTTCCTT GTTTTGGGGT TTAGTAATGA AAATGAATCT
    AATATTGCAA ATATCATAAG AGAAAATGCT GGAAAAGTCG TGTCCCTTCA GAGCAGAATT
    GTTGCTGATT ATGCTGTGGT TCCTCTGCTG GGGTGTGAAG TAGAAGCAAC TGTAGGAGAA
    GTCGTCACAA ATACATGGCT GGTAACTTGT ATAGACTATC AGACTTTAAT TGATCCAAAG
    TCAAATCCTC TCTTCATGCC AGTTCCATTT ATGGCAGGAA TGACTCCTTT AGAGGATTGT
    GTTATTTCAT TCAGCCAGTG TGCAGGAGCA GAAAAAGATT CATTGACATT CTTAGCAAAT
    CGCCTTGGAG CTAGCGTTCA AGAATTCTTT GTTCGCAAAT CCAATGCAAA GAAAGGCATG
    TTTGCCAGTA CACATCTTGT ATTGAAAGAA CCAGGTGGTT CAAAATATGA AGCTGCAAAG
    AAGTGGAATT TGCCAGCAGT CACCATATCT TGGCTGTTGG AGACTGCTAG AATGGGAAAG
    AGAGCAAATG AAAACCATTT TCTGATTGAA AAGTCAACTA AAGAAGAACA AAGTTTGGAA
    ACTGAAATAA CAAACAAGGT GAATCTAAAT CCAGATACTC CAGAGCATCC TGTTGCACAC
    CTGGAAACTC AGAGGAAAAC AGCTGTTACA CCTTTAGATA TGAACCGCTT TCAGAGTAGA
    GCTTTCCATG CTGTGATCTC ACAACACACC AAACAGGTTT CGACCTCCTC GCCAGTGGGA
    CAGCCACTTC AGAAGGAGCC CTCCTTACAT CTGGACACAC CATCAAAGTT TCTGTCCAAG
    GACAAACTCT TCAAGCCTTC CTTTGATGTG AAGGATGCAC TTGCAGCCTT GGAAACTCCA
    GGAGGTCCCA GCCAGCAGAA AAGGAAACTG AGTACACCAC TTTCAGAAGT CATTGTCAGA
    AACTTGAAAC TTGCTTTAGC AAATAGCTCT CGAAATGCTG TTGCTCTTTC TGCCAGCCCT
    CGGCTGAAGG ATGCCCAATC AGAGAAGGAT GAAGCTCCAA AGCCACTTCA TAAGGTAGTG
    GTATGTGTGA GTAAGAAACT CAGTAAGAAG CAGAGTGAAC TGAATGGGAT CGCAGCCTCT
    TTAGGAGCAG ATTACAGATG GAGTTTTGAT GAAACCGTGA CTCATTTCAT TTATCAAGGC
    CGACCAAATG ACACTAACCG AGAGTATAAA TCTGTAAAAG AAAGAGGAGT ACACATTGTT
    TCAGAGCACT GGCTTTTAGA ATGTGCCCAA GAGTATAAAC ATCTTCCTGA ATCTCTTTAT
    CCACATACTT ATAATCCCAA AATGAGCTTG GATATCAGTA CAGTGCAAGA TGGCAGAATC
    TCTAATAGTC GACTGCTCTC AGCTGATTCA ACGACAAAGG ATGATGAGCC AGATCATTTG
    CCTGGAGAAG AAAACGATAT AGACAATATG ACCACCAGTA ATAAAGAATC CGCAACATCA
    AATGGAGATG GAAGGAATGA CTCTAAAGGA GCTCTGACAC AGACTTTGGA GATGAGAGAG
    AATTTTCAAA AGCAGCTGCA AGAGATAATG TCTGCAACAT CAATAGTGAA ACCTCAGGGA
    CCAAGGGCTT CCCTTTCAAG AAGTGGTTGT AACAGTGCTT CTTCAACCCC TGATAGCACT
    CGCTCTGCCC GCAGTGGGCG AAGTAGAGTC CTGGAGGCAC TGAGGCAGTC TCGTCAAACG
    GTACCTGATA TCAACACAGA GCCCTCCCAG AATGAACAGA TCATTTGGGA TGACCCGACA
    GCCAGGGAAG AGAGAGCAAG GCTTGCCAGC AATCTGCAGT GGCCTAGTTG TCCCACACAG
    TATTCTGAGC TTCAGGTTGA CATTAAAAAA TTGGAGGATT CTCCTTTCCA GGAGCCTTTA
    CATGATCCAG AAATTGCTGA ACAGGCAGTC TGTGATCCCG GAAATGTGTG TGTGACTGAA
    GCCCCAAAAC ACCCGACCTC TGAAGAACTG GAAACACCAG TAAAAGACAG CCACCTGATC
    CCTACACCTC AAGCTCCCAG TATTGCCTTC CCCCTAGCCA ACCCACCTGT GGCTCCACAC
    CCTAGAGAAA AGATTATAAC AATAGAGGAA ACCCATGAAG ACTTGAAAAA ACAGTACATA
    TTTCAGTTGT CGTCTCTGAA TCCTCAAGAA CGTATTGATT ATTGTCATCT GATTGAAAAA
    CTAGGTGGAT TAGTAATAGA GAAGCAATGC TTTGATCCCA ACTGTACACA CATTGTTGTG
    GGACATCCTC TTCGAAACGA GAAGTATTTA GCTTCAGTGG CAGCTGGGAA GTGGGTACTT
    CATCGCTCCT ACCTGGAAGC ATGCCGGACT GCTGGGCACT TCGTGCTGGA AGAAGACTAT
    GAATGGGGAA GTAGTTCCAT ACTTGATGTT TTGACTGGAA TCAATGTACA GCAACGAAGA
    CTAGCACTTG CAGCAATGAG ATGGAGGAAA AAAATCCAGC AAAGACAAGA ATCAGGCATT
    GTTGAGGGGG CATTTAGTGG GTGGAAGGTT ATCTTACATG TTGACCAGTC CCGAGAAGCA
    GGATTCAAAC GCCTTCTTCA GTCAGGAGGA GCAAAGGTAC TACCTGGTCA TTCTGTACCT
    TTATTTAAAG AAGCTACACA TCTCTTTTCT GACTTCAATA AACTGAAACC AGATGACTCA
    GGAGTTAATA TAGCAGAAGC TGCTGCCCAA AATGTGTACT GCTTGAAAAC AGAGTACATT
    GCTGATTATC TGATGCAGGA ATCACCTCCT CCAGTAGAAA ATTACTGTCT ACCAGAAGCA
    GTTTCATTTC TTCAGAATAA TAAGGAACCT GGGCCTGGAT TATCACAGAA GAGGAAAGCT
    CCTACAGAGA AAAATAAAAT CAAACGACCT AGAGTAAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24162
    Clone ID Related Accession (Same CDS sequence) XM_005634445.3 , XM_005634445.2
    Accession Version XM_005634445.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product DNA topoisomerase 2-binding protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005634445.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTCTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    GAATGTTTTT TTAAAGCTCT GGAGTCAATA AAAGAATTTC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA CAGGAAAATG ATAGGTCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGC
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAACGATGTG TCCCAAGAGC TGAACATCCA
    GTCTATAATA TGGTTATGTC TGATGTAACT ATATCTTGTA CAAGCCTGGA AAAAGACAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GTGTATATAG AGATCTTAAT
    ATATCAGTAA CTCATCTTAT TGCAGGAGAA GTTGGCAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCGATTTT GCTTCCTTCT TGGATTAAAA CACTTTGGGA AAAGTCACAA
    GAGAAAAAAA TATCTAGATA TACTGATGTA AACATGGAAG ACTTCAAGTG CCCAATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGGTTG TGTAGCTTAG ATAGGAAGGC AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATATATG GGACAACTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT GTTGACAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ACCAGAAACA AAGACTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCC ACACAGTTGA CAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGTT GCATAAATGA ATCAATGTGT AATTCAGTGC TTAATAGCAA AGTGGAGCCT
    ACCCTTGAAA ATCTAGAAAA TTTGGATGTC AGTGCATTTC AAGCACCTGA AGATTTATTA
    GATGGTTGTC GGATCTATCT TTGCGGTTTT AGTGGCAGAA GGCTAGATAA ACTAAGAAGG
    CTTATTAACA GTGGAGGTGG AGTTCGTTTT AATCAGCTCA ATGAAGATGT AACTCATGTT
    ATAGTGGGAG ATTATGATGA TGAATTGAAG CAGTTTTGGG ATAAATCGGC CCACAGGCCT
    CATGTAGTAG GAGCAAAATG GTTGCTGGAG TGTTTTAGTA AAGGTTATAT GCTTCCTGAA
    GAAACATATA TCCATGCTAA CTACCAGCCA GTGGAAATTC CAGTTTCAGA TCAACCTGAA
    AATAAAACAG CCCTTTTAAA AAAGAACAAC AGCTTTTCTA AGAAAGACTT TGCTTCTGAT
    GAAAAGCATG AACAAGCTGA TGAAGATCTG CTCTCTCAAT ATGTAAATAA TAACCCGACA
    GTAGGTGAAG CTGTGAAGTC TGAAGTTGAG CCCTTTAATG ACTCTACTCA TATTTCTCCG
    CAAAAAGAAA ACCAGTCTTC TGCCACTCAT TGCCTCCCTG ATGCTTCTAC AACTACTGAA
    GAAGGTTTAT TTAGCCAAAA GAGTTTCCTT GTTTTGGGGT TTAGTAATGA AAATGAATCT
    AATATTGCAA ATATCATAAG AGAAAATGCT GGAAAAGTCG TGTCCCTTCA GAGCAGAATT
    GTTGCTGATT ATGCTGTGGT TCCTCTGCTG GGGTGTGAAG TAGAAGCAAC TGTAGGAGAA
    GTCGTCACAA ATACATGGCT GGTAACTTGT ATAGACTATC AGACTTTAAT TGATCCAAAG
    TCAAATCCTC TCTTCATGCC AGTTCCATTT ATGGCAGGAA TGACTCCTTT AGAGGATTGT
    GTTATTTCAT TCAGCCAGTG TGCAGGAGCA GAAAAAGATT CATTGACATT CTTAGCAAAT
    CGCCTTGGAG CTAGCGTTCA AGAATTCTTT GTTCGCAAAT CCAATGCAAA GAAAGGCATG
    TTTGCCAGTA CACATCTTGT ATTGAAAGAA CCAGGTGGTT CAAAATATGA AGCTGCAAAG
    AAGTGGAATT TGCCAGCAGT CACCATATCT TGGCTGTTGG AGACTGCTAG AATGGGAAAG
    AGAGCAAATG AAAACCATTT TCTGATTGAA AAGTCAACTA AAGAAGAACA AAGTTTGGAA
    ACTGAAATAA CAAACAAGGT GAATCTAAAT CCAGATACTC CAGAGCATCC TGTTGCACAC
    CTGGAAACTC AGAGGAAAAC AGCTGTTACA CCTTTAGATA TGAACCGCTT TCAGAGTAGA
    GCTTTCCATG CTGTGATCTC ACAACACACC AAACAGGTTT CGACCTCCTC GCCAGTGGGA
    CAGCCACTTC AGAAGGAGCC CTCCTTACAT CTGGACACAC CATCAAAGTT TCTGTCCAAG
    GACAAACTCT TCAAGCCTTC CTTTGATGTG AAGGATGCAC TTGCAGCCTT GGAAACTCCA
    GGAGGTCCCA GCCAGCAGAA AAGGAAACTG AGTACACCAC TTTCAGAAGT CATTGTCAGA
    AACTTGAAAC TTGCTTTAGC AAATAGCTCT CGAAATGCTG TTGCTCTTTC TGCCAGCCCT
    CGGCTGAAGG ATGCCCAATC AGAGAAGGAT GAAGCTCCAA AGCCACTTCA TAAGGTAGTG
    GTATGTGTGA GTAAGAAACT CAGTAAGAAG CAGAGTGAAC TGAATGGGAT CGCAGCCTCT
    TTAGGAGCAG ATTACAGATG GAGTTTTGAT GAAACCGTGA CTCATTTCAT TTATCAAGGC
    CGACCAAATG ACACTAACCG AGAGTATAAA TCTGTAAAAG AAAGAGGAGT ACACATTGTT
    TCAGAGCACT GGCTTTTAGA ATGTGCCCAA GAGTATAAAC ATCTTCCTGA ATCTCTTTAT
    CCACATACTT ATAATCCCAA AATGAGCTTG GATATCAGTA CAGTGCAAGA TGGCAGAATC
    TCTAATAGTC GACTGCTCTC AGCTGATTCA ACGACAAAGG ATGATGAGCC AGATCATTTG
    CCTGGAGAAG AAAACGATAT AGACAATATG ACCACCAGTA ATAAAGAATC CGCAACATCA
    AATGGAGATG GAAGGAATGA CTCTAAAGGA GCTCTGACAC AGACTTTGGA GATGAGAGAG
    AATTTTCAAA AGCAGCTGCA AGAGATAATG TCTGCAACAT CAATAGTGAA ACCTCAGGGA
    CCAAGGGCTT CCCTTTCAAG AAGTGGTTGT AACAGTGCTT CTTCAACCCC TGATAGCACT
    CGCTCTGCCC GCAGTGGGCG AAGTAGAGTC CTGGAGGCAC TGAGGCAGTC TCGTCAAACG
    GTACCTGATA TCAACACAGA GCCCTCCCAG AATGAACAGA TCATTTGGGA TGACCCGACA
    GCCAGGGAAG AGAGAGCAAG GCTTGCCAGC AATCTGCAGT GGCCTAGTTG TCCCACACAG
    TATTCTGAGC TTCAGGTTGA CATTAAAAAA TTGGAGGATT CTCCTTTCCA GGAGCCTTTA
    CATGATCCAG AAATTGCTGA ACAGGTCTGT GATCCCGGAA ATGTGTGTGT GACTGAAGCC
    CCAAAACACC CGACCTCTGA AGAACTGGAA ACACCAGTAA AAGACAGCCA CCTGATCCCT
    ACACCTCAAG CTCCCAGTAT TGCCTTCCCC CTAGCCAACC CACCTGTGGC TCCACACCCT
    AGAGAAAAGA TTATAACAAT AGAGGAAACC CATGAAGACT TGAAAAAACA GTACATATTT
    CAGTTGTCGT CTCTGAATCC TCAAGAACGT ATTGATTATT GTCATCTGAT TGAAAAACTA
    GGTGGATTAG TAATAGAGAA GCAATGCTTT GATCCCAACT GTACACACAT TGTTGTGGGA
    CATCCTCTTC GAAACGAGAA GTATTTAGCT TCAGTGGCAG CTGGGAAGTG GGTACTTCAT
    CGCTCCTACC TGGAAGCATG CCGGACTGCT GGGCACTTCG TGCTGGAAGA AGACTATGAA
    TGGGGAAGTA GTTCCATACT TGATGTTTTG ACTGGAATCA ATGTACAGCA ACGAAGACTA
    GCACTTGCAG CAATGAGATG GAGGAAAAAA ATCCAGCAAA GACAAGAATC AGGCATTGTT
    GAGGGGGCAT TTAGTGGGTG GAAGGTTATC TTACATGTTG ACCAGTCCCG AGAAGCAGGA
    TTCAAACGCC TTCTTCAGTC AGGAGGAGCA AAGGTACTAC CTGGTCATTC TGTACCTTTA
    TTTAAAGAAG CTACACATCT CTTTTCTGAC TTCAATAAAC TGAAACCAGA TGACTCAGGA
    GTTAATATAG CAGAAGCTGC TGCCCAAAAT GTGTACTGCT TGAAAACAGA GTACATTGCT
    GATTATCTGA TGCAGGAATC ACCTCCTCCA GTAGAAAATT ACTGTCTACC AGAAGCAGTT
    TCATTTCTTC AGAATAATAA GGAACCTGGG CCTGGATTAT CACAGAAGAG GAAAGCTCCT
    ACAGAGAAAA ATAAAATCAA ACGACCTAGA GTAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634502.1
    CDS143..4681
    Translation

    Target ORF information:

    RefSeq Version XM_005634445.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005634445.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTCTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    GAATGTTTTT TTAAAGCTCT GGAGTCAATA AAAGAATTTC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA CAGGAAAATG ATAGGTCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGC
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAACGATGTG TCCCAAGAGC TGAACATCCA
    GTCTATAATA TGGTTATGTC TGATGTAACT ATATCTTGTA CAAGCCTGGA AAAAGACAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GTGTATATAG AGATCTTAAT
    ATATCAGTAA CTCATCTTAT TGCAGGAGAA GTTGGCAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCGATTTT GCTTCCTTCT TGGATTAAAA CACTTTGGGA AAAGTCACAA
    GAGAAAAAAA TATCTAGATA TACTGATGTA AACATGGAAG ACTTCAAGTG CCCAATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGGTTG TGTAGCTTAG ATAGGAAGGC AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATATATG GGACAACTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT GTTGACAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ACCAGAAACA AAGACTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCC ACACAGTTGA CAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGTT GCATAAATGA ATCAATGTGT AATTCAGTGC TTAATAGCAA AGTGGAGCCT
    ACCCTTGAAA ATCTAGAAAA TTTGGATGTC AGTGCATTTC AAGCACCTGA AGATTTATTA
    GATGGTTGTC GGATCTATCT TTGCGGTTTT AGTGGCAGAA GGCTAGATAA ACTAAGAAGG
    CTTATTAACA GTGGAGGTGG AGTTCGTTTT AATCAGCTCA ATGAAGATGT AACTCATGTT
    ATAGTGGGAG ATTATGATGA TGAATTGAAG CAGTTTTGGG ATAAATCGGC CCACAGGCCT
    CATGTAGTAG GAGCAAAATG GTTGCTGGAG TGTTTTAGTA AAGGTTATAT GCTTCCTGAA
    GAAACATATA TCCATGCTAA CTACCAGCCA GTGGAAATTC CAGTTTCAGA TCAACCTGAA
    AATAAAACAG CCCTTTTAAA AAAGAACAAC AGCTTTTCTA AGAAAGACTT TGCTTCTGAT
    GAAAAGCATG AACAAGCTGA TGAAGATCTG CTCTCTCAAT ATGTAAATAA TAACCCGACA
    GTAGGTGAAG CTGTGAAGTC TGAAGTTGAG CCCTTTAATG ACTCTACTCA TATTTCTCCG
    CAAAAAGAAA ACCAGTCTTC TGCCACTCAT TGCCTCCCTG ATGCTTCTAC AACTACTGAA
    GAAGGTTTAT TTAGCCAAAA GAGTTTCCTT GTTTTGGGGT TTAGTAATGA AAATGAATCT
    AATATTGCAA ATATCATAAG AGAAAATGCT GGAAAAGTCG TGTCCCTTCA GAGCAGAATT
    GTTGCTGATT ATGCTGTGGT TCCTCTGCTG GGGTGTGAAG TAGAAGCAAC TGTAGGAGAA
    GTCGTCACAA ATACATGGCT GGTAACTTGT ATAGACTATC AGACTTTAAT TGATCCAAAG
    TCAAATCCTC TCTTCATGCC AGTTCCATTT ATGGCAGGAA TGACTCCTTT AGAGGATTGT
    GTTATTTCAT TCAGCCAGTG TGCAGGAGCA GAAAAAGATT CATTGACATT CTTAGCAAAT
    CGCCTTGGAG CTAGCGTTCA AGAATTCTTT GTTCGCAAAT CCAATGCAAA GAAAGGCATG
    TTTGCCAGTA CACATCTTGT ATTGAAAGAA CCAGGTGGTT CAAAATATGA AGCTGCAAAG
    AAGTGGAATT TGCCAGCAGT CACCATATCT TGGCTGTTGG AGACTGCTAG AATGGGAAAG
    AGAGCAAATG AAAACCATTT TCTGATTGAA AAGTCAACTA AAGAAGAACA AAGTTTGGAA
    ACTGAAATAA CAAACAAGGT GAATCTAAAT CCAGATACTC CAGAGCATCC TGTTGCACAC
    CTGGAAACTC AGAGGAAAAC AGCTGTTACA CCTTTAGATA TGAACCGCTT TCAGAGTAGA
    GCTTTCCATG CTGTGATCTC ACAACACACC AAACAGGTTT CGACCTCCTC GCCAGTGGGA
    CAGCCACTTC AGAAGGAGCC CTCCTTACAT CTGGACACAC CATCAAAGTT TCTGTCCAAG
    GACAAACTCT TCAAGCCTTC CTTTGATGTG AAGGATGCAC TTGCAGCCTT GGAAACTCCA
    GGAGGTCCCA GCCAGCAGAA AAGGAAACTG AGTACACCAC TTTCAGAAGT CATTGTCAGA
    AACTTGAAAC TTGCTTTAGC AAATAGCTCT CGAAATGCTG TTGCTCTTTC TGCCAGCCCT
    CGGCTGAAGG ATGCCCAATC AGAGAAGGAT GAAGCTCCAA AGCCACTTCA TAAGGTAGTG
    GTATGTGTGA GTAAGAAACT CAGTAAGAAG CAGAGTGAAC TGAATGGGAT CGCAGCCTCT
    TTAGGAGCAG ATTACAGATG GAGTTTTGAT GAAACCGTGA CTCATTTCAT TTATCAAGGC
    CGACCAAATG ACACTAACCG AGAGTATAAA TCTGTAAAAG AAAGAGGAGT ACACATTGTT
    TCAGAGCACT GGCTTTTAGA ATGTGCCCAA GAGTATAAAC ATCTTCCTGA ATCTCTTTAT
    CCACATACTT ATAATCCCAA AATGAGCTTG GATATCAGTA CAGTGCAAGA TGGCAGAATC
    TCTAATAGTC GACTGCTCTC AGCTGATTCA ACGACAAAGG ATGATGAGCC AGATCATTTG
    CCTGGAGAAG AAAACGATAT AGACAATATG ACCACCAGTA ATAAAGAATC CGCAACATCA
    AATGGAGATG GAAGGAATGA CTCTAAAGGA GCTCTGACAC AGACTTTGGA GATGAGAGAG
    AATTTTCAAA AGCAGCTGCA AGAGATAATG TCTGCAACAT CAATAGTGAA ACCTCAGGGA
    CCAAGGGCTT CCCTTTCAAG AAGTGGTTGT AACAGTGCTT CTTCAACCCC TGATAGCACT
    CGCTCTGCCC GCAGTGGGCG AAGTAGAGTC CTGGAGGCAC TGAGGCAGTC TCGTCAAACG
    GTACCTGATA TCAACACAGA GCCCTCCCAG AATGAACAGA TCATTTGGGA TGACCCGACA
    GCCAGGGAAG AGAGAGCAAG GCTTGCCAGC AATCTGCAGT GGCCTAGTTG TCCCACACAG
    TATTCTGAGC TTCAGGTTGA CATTAAAAAA TTGGAGGATT CTCCTTTCCA GGAGCCTTTA
    CATGATCCAG AAATTGCTGA ACAGGTCTGT GATCCCGGAA ATGTGTGTGT GACTGAAGCC
    CCAAAACACC CGACCTCTGA AGAACTGGAA ACACCAGTAA AAGACAGCCA CCTGATCCCT
    ACACCTCAAG CTCCCAGTAT TGCCTTCCCC CTAGCCAACC CACCTGTGGC TCCACACCCT
    AGAGAAAAGA TTATAACAAT AGAGGAAACC CATGAAGACT TGAAAAAACA GTACATATTT
    CAGTTGTCGT CTCTGAATCC TCAAGAACGT ATTGATTATT GTCATCTGAT TGAAAAACTA
    GGTGGATTAG TAATAGAGAA GCAATGCTTT GATCCCAACT GTACACACAT TGTTGTGGGA
    CATCCTCTTC GAAACGAGAA GTATTTAGCT TCAGTGGCAG CTGGGAAGTG GGTACTTCAT
    CGCTCCTACC TGGAAGCATG CCGGACTGCT GGGCACTTCG TGCTGGAAGA AGACTATGAA
    TGGGGAAGTA GTTCCATACT TGATGTTTTG ACTGGAATCA ATGTACAGCA ACGAAGACTA
    GCACTTGCAG CAATGAGATG GAGGAAAAAA ATCCAGCAAA GACAAGAATC AGGCATTGTT
    GAGGGGGCAT TTAGTGGGTG GAAGGTTATC TTACATGTTG ACCAGTCCCG AGAAGCAGGA
    TTCAAACGCC TTCTTCAGTC AGGAGGAGCA AAGGTACTAC CTGGTCATTC TGTACCTTTA
    TTTAAAGAAG CTACACATCT CTTTTCTGAC TTCAATAAAC TGAAACCAGA TGACTCAGGA
    GTTAATATAG CAGAAGCTGC TGCCCAAAAT GTGTACTGCT TGAAAACAGA GTACATTGCT
    GATTATCTGA TGCAGGAATC ACCTCCTCCA GTAGAAAATT ACTGTCTACC AGAAGCAGTT
    TCATTTCTTC AGAATAATAA GGAACCTGGG CCTGGATTAT CACAGAAGAG GAAAGCTCCT
    ACAGAGAAAA ATAAAATCAA ACGACCTAGA GTAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24162
    Clone ID Related Accession (Same CDS sequence) XM_005634445.3 , XM_005634445.2
    Accession Version XM_005634445.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DNA topoisomerase 2-binding protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005634445.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTCTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    GAATGTTTTT TTAAAGCTCT GGAGTCAATA AAAGAATTTC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA CAGGAAAATG ATAGGTCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGC
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAACGATGTG TCCCAAGAGC TGAACATCCA
    GTCTATAATA TGGTTATGTC TGATGTAACT ATATCTTGTA CAAGCCTGGA AAAAGACAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GTGTATATAG AGATCTTAAT
    ATATCAGTAA CTCATCTTAT TGCAGGAGAA GTTGGCAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCGATTTT GCTTCCTTCT TGGATTAAAA CACTTTGGGA AAAGTCACAA
    GAGAAAAAAA TATCTAGATA TACTGATGTA AACATGGAAG ACTTCAAGTG CCCAATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGGTTG TGTAGCTTAG ATAGGAAGGC AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATATATG GGACAACTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT GTTGACAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ACCAGAAACA AAGACTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCC ACACAGTTGA CAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGTT GCATAAATGA ATCAATGTGT AATTCAGTGC TTAATAGCAA AGTGGAGCCT
    ACCCTTGAAA ATCTAGAAAA TTTGGATGTC AGTGCATTTC AAGCACCTGA AGATTTATTA
    GATGGTTGTC GGATCTATCT TTGCGGTTTT AGTGGCAGAA GGCTAGATAA ACTAAGAAGG
    CTTATTAACA GTGGAGGTGG AGTTCGTTTT AATCAGCTCA ATGAAGATGT AACTCATGTT
    ATAGTGGGAG ATTATGATGA TGAATTGAAG CAGTTTTGGG ATAAATCGGC CCACAGGCCT
    CATGTAGTAG GAGCAAAATG GTTGCTGGAG TGTTTTAGTA AAGGTTATAT GCTTCCTGAA
    GAAACATATA TCCATGCTAA CTACCAGCCA GTGGAAATTC CAGTTTCAGA TCAACCTGAA
    AATAAAACAG CCCTTTTAAA AAAGAACAAC AGCTTTTCTA AGAAAGACTT TGCTTCTGAT
    GAAAAGCATG AACAAGCTGA TGAAGATCTG CTCTCTCAAT ATGTAAATAA TAACCCGACA
    GTAGGTGAAG CTGTGAAGTC TGAAGTTGAG CCCTTTAATG ACTCTACTCA TATTTCTCCG
    CAAAAAGAAA ACCAGTCTTC TGCCACTCAT TGCCTCCCTG ATGCTTCTAC AACTACTGAA
    GAAGGTTTAT TTAGCCAAAA GAGTTTCCTT GTTTTGGGGT TTAGTAATGA AAATGAATCT
    AATATTGCAA ATATCATAAG AGAAAATGCT GGAAAAGTCG TGTCCCTTCA GAGCAGAATT
    GTTGCTGATT ATGCTGTGGT TCCTCTGCTG GGGTGTGAAG TAGAAGCAAC TGTAGGAGAA
    GTCGTCACAA ATACATGGCT GGTAACTTGT ATAGACTATC AGACTTTAAT TGATCCAAAG
    TCAAATCCTC TCTTCATGCC AGTTCCATTT ATGGCAGGAA TGACTCCTTT AGAGGATTGT
    GTTATTTCAT TCAGCCAGTG TGCAGGAGCA GAAAAAGATT CATTGACATT CTTAGCAAAT
    CGCCTTGGAG CTAGCGTTCA AGAATTCTTT GTTCGCAAAT CCAATGCAAA GAAAGGCATG
    TTTGCCAGTA CACATCTTGT ATTGAAAGAA CCAGGTGGTT CAAAATATGA AGCTGCAAAG
    AAGTGGAATT TGCCAGCAGT CACCATATCT TGGCTGTTGG AGACTGCTAG AATGGGAAAG
    AGAGCAAATG AAAACCATTT TCTGATTGAA AAGTCAACTA AAGAAGAACA AAGTTTGGAA
    ACTGAAATAA CAAACAAGGT GAATCTAAAT CCAGATACTC CAGAGCATCC TGTTGCACAC
    CTGGAAACTC AGAGGAAAAC AGCTGTTACA CCTTTAGATA TGAACCGCTT TCAGAGTAGA
    GCTTTCCATG CTGTGATCTC ACAACACACC AAACAGGTTT CGACCTCCTC GCCAGTGGGA
    CAGCCACTTC AGAAGGAGCC CTCCTTACAT CTGGACACAC CATCAAAGTT TCTGTCCAAG
    GACAAACTCT TCAAGCCTTC CTTTGATGTG AAGGATGCAC TTGCAGCCTT GGAAACTCCA
    GGAGGTCCCA GCCAGCAGAA AAGGAAACTG AGTACACCAC TTTCAGAAGT CATTGTCAGA
    AACTTGAAAC TTGCTTTAGC AAATAGCTCT CGAAATGCTG TTGCTCTTTC TGCCAGCCCT
    CGGCTGAAGG ATGCCCAATC AGAGAAGGAT GAAGCTCCAA AGCCACTTCA TAAGGTAGTG
    GTATGTGTGA GTAAGAAACT CAGTAAGAAG CAGAGTGAAC TGAATGGGAT CGCAGCCTCT
    TTAGGAGCAG ATTACAGATG GAGTTTTGAT GAAACCGTGA CTCATTTCAT TTATCAAGGC
    CGACCAAATG ACACTAACCG AGAGTATAAA TCTGTAAAAG AAAGAGGAGT ACACATTGTT
    TCAGAGCACT GGCTTTTAGA ATGTGCCCAA GAGTATAAAC ATCTTCCTGA ATCTCTTTAT
    CCACATACTT ATAATCCCAA AATGAGCTTG GATATCAGTA CAGTGCAAGA TGGCAGAATC
    TCTAATAGTC GACTGCTCTC AGCTGATTCA ACGACAAAGG ATGATGAGCC AGATCATTTG
    CCTGGAGAAG AAAACGATAT AGACAATATG ACCACCAGTA ATAAAGAATC CGCAACATCA
    AATGGAGATG GAAGGAATGA CTCTAAAGGA GCTCTGACAC AGACTTTGGA GATGAGAGAG
    AATTTTCAAA AGCAGCTGCA AGAGATAATG TCTGCAACAT CAATAGTGAA ACCTCAGGGA
    CCAAGGGCTT CCCTTTCAAG AAGTGGTTGT AACAGTGCTT CTTCAACCCC TGATAGCACT
    CGCTCTGCCC GCAGTGGGCG AAGTAGAGTC CTGGAGGCAC TGAGGCAGTC TCGTCAAACG
    GTACCTGATA TCAACACAGA GCCCTCCCAG AATGAACAGA TCATTTGGGA TGACCCGACA
    GCCAGGGAAG AGAGAGCAAG GCTTGCCAGC AATCTGCAGT GGCCTAGTTG TCCCACACAG
    TATTCTGAGC TTCAGGTTGA CATTAAAAAA TTGGAGGATT CTCCTTTCCA GGAGCCTTTA
    CATGATCCAG AAATTGCTGA ACAGGTCTGT GATCCCGGAA ATGTGTGTGT GACTGAAGCC
    CCAAAACACC CGACCTCTGA AGAACTGGAA ACACCAGTAA AAGACAGCCA CCTGATCCCT
    ACACCTCAAG CTCCCAGTAT TGCCTTCCCC CTAGCCAACC CACCTGTGGC TCCACACCCT
    AGAGAAAAGA TTATAACAAT AGAGGAAACC CATGAAGACT TGAAAAAACA GTACATATTT
    CAGTTGTCGT CTCTGAATCC TCAAGAACGT ATTGATTATT GTCATCTGAT TGAAAAACTA
    GGTGGATTAG TAATAGAGAA GCAATGCTTT GATCCCAACT GTACACACAT TGTTGTGGGA
    CATCCTCTTC GAAACGAGAA GTATTTAGCT TCAGTGGCAG CTGGGAAGTG GGTACTTCAT
    CGCTCCTACC TGGAAGCATG CCGGACTGCT GGGCACTTCG TGCTGGAAGA AGACTATGAA
    TGGGGAAGTA GTTCCATACT TGATGTTTTG ACTGGAATCA ATGTACAGCA ACGAAGACTA
    GCACTTGCAG CAATGAGATG GAGGAAAAAA ATCCAGCAAA GACAAGAATC AGGCATTGTT
    GAGGGGGCAT TTAGTGGGTG GAAGGTTATC TTACATGTTG ACCAGTCCCG AGAAGCAGGA
    TTCAAACGCC TTCTTCAGTC AGGAGGAGCA AAGGTACTAC CTGGTCATTC TGTACCTTTA
    TTTAAAGAAG CTACACATCT CTTTTCTGAC TTCAATAAAC TGAAACCAGA TGACTCAGGA
    GTTAATATAG CAGAAGCTGC TGCCCAAAAT GTGTACTGCT TGAAAACAGA GTACATTGCT
    GATTATCTGA TGCAGGAATC ACCTCCTCCA GTAGAAAATT ACTGTCTACC AGAAGCAGTT
    TCATTTCTTC AGAATAATAA GGAACCTGGG CCTGGATTAT CACAGAAGAG GAAAGCTCCT
    ACAGAGAAAA ATAAAATCAA ACGACCTAGA GTAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634502.1
    CDS184..4722
    Translation

    Target ORF information:

    RefSeq Version XM_005634445.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005634445.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTCTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    GAATGTTTTT TTAAAGCTCT GGAGTCAATA AAAGAATTTC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA CAGGAAAATG ATAGGTCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGC
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAACGATGTG TCCCAAGAGC TGAACATCCA
    GTCTATAATA TGGTTATGTC TGATGTAACT ATATCTTGTA CAAGCCTGGA AAAAGACAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GTGTATATAG AGATCTTAAT
    ATATCAGTAA CTCATCTTAT TGCAGGAGAA GTTGGCAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCGATTTT GCTTCCTTCT TGGATTAAAA CACTTTGGGA AAAGTCACAA
    GAGAAAAAAA TATCTAGATA TACTGATGTA AACATGGAAG ACTTCAAGTG CCCAATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGGTTG TGTAGCTTAG ATAGGAAGGC AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATATATG GGACAACTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT GTTGACAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ACCAGAAACA AAGACTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCC ACACAGTTGA CAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGTT GCATAAATGA ATCAATGTGT AATTCAGTGC TTAATAGCAA AGTGGAGCCT
    ACCCTTGAAA ATCTAGAAAA TTTGGATGTC AGTGCATTTC AAGCACCTGA AGATTTATTA
    GATGGTTGTC GGATCTATCT TTGCGGTTTT AGTGGCAGAA GGCTAGATAA ACTAAGAAGG
    CTTATTAACA GTGGAGGTGG AGTTCGTTTT AATCAGCTCA ATGAAGATGT AACTCATGTT
    ATAGTGGGAG ATTATGATGA TGAATTGAAG CAGTTTTGGG ATAAATCGGC CCACAGGCCT
    CATGTAGTAG GAGCAAAATG GTTGCTGGAG TGTTTTAGTA AAGGTTATAT GCTTCCTGAA
    GAAACATATA TCCATGCTAA CTACCAGCCA GTGGAAATTC CAGTTTCAGA TCAACCTGAA
    AATAAAACAG CCCTTTTAAA AAAGAACAAC AGCTTTTCTA AGAAAGACTT TGCTTCTGAT
    GAAAAGCATG AACAAGCTGA TGAAGATCTG CTCTCTCAAT ATGTAAATAA TAACCCGACA
    GTAGGTGAAG CTGTGAAGTC TGAAGTTGAG CCCTTTAATG ACTCTACTCA TATTTCTCCG
    CAAAAAGAAA ACCAGTCTTC TGCCACTCAT TGCCTCCCTG ATGCTTCTAC AACTACTGAA
    GAAGGTTTAT TTAGCCAAAA GAGTTTCCTT GTTTTGGGGT TTAGTAATGA AAATGAATCT
    AATATTGCAA ATATCATAAG AGAAAATGCT GGAAAAGTCG TGTCCCTTCA GAGCAGAATT
    GTTGCTGATT ATGCTGTGGT TCCTCTGCTG GGGTGTGAAG TAGAAGCAAC TGTAGGAGAA
    GTCGTCACAA ATACATGGCT GGTAACTTGT ATAGACTATC AGACTTTAAT TGATCCAAAG
    TCAAATCCTC TCTTCATGCC AGTTCCATTT ATGGCAGGAA TGACTCCTTT AGAGGATTGT
    GTTATTTCAT TCAGCCAGTG TGCAGGAGCA GAAAAAGATT CATTGACATT CTTAGCAAAT
    CGCCTTGGAG CTAGCGTTCA AGAATTCTTT GTTCGCAAAT CCAATGCAAA GAAAGGCATG
    TTTGCCAGTA CACATCTTGT ATTGAAAGAA CCAGGTGGTT CAAAATATGA AGCTGCAAAG
    AAGTGGAATT TGCCAGCAGT CACCATATCT TGGCTGTTGG AGACTGCTAG AATGGGAAAG
    AGAGCAAATG AAAACCATTT TCTGATTGAA AAGTCAACTA AAGAAGAACA AAGTTTGGAA
    ACTGAAATAA CAAACAAGGT GAATCTAAAT CCAGATACTC CAGAGCATCC TGTTGCACAC
    CTGGAAACTC AGAGGAAAAC AGCTGTTACA CCTTTAGATA TGAACCGCTT TCAGAGTAGA
    GCTTTCCATG CTGTGATCTC ACAACACACC AAACAGGTTT CGACCTCCTC GCCAGTGGGA
    CAGCCACTTC AGAAGGAGCC CTCCTTACAT CTGGACACAC CATCAAAGTT TCTGTCCAAG
    GACAAACTCT TCAAGCCTTC CTTTGATGTG AAGGATGCAC TTGCAGCCTT GGAAACTCCA
    GGAGGTCCCA GCCAGCAGAA AAGGAAACTG AGTACACCAC TTTCAGAAGT CATTGTCAGA
    AACTTGAAAC TTGCTTTAGC AAATAGCTCT CGAAATGCTG TTGCTCTTTC TGCCAGCCCT
    CGGCTGAAGG ATGCCCAATC AGAGAAGGAT GAAGCTCCAA AGCCACTTCA TAAGGTAGTG
    GTATGTGTGA GTAAGAAACT CAGTAAGAAG CAGAGTGAAC TGAATGGGAT CGCAGCCTCT
    TTAGGAGCAG ATTACAGATG GAGTTTTGAT GAAACCGTGA CTCATTTCAT TTATCAAGGC
    CGACCAAATG ACACTAACCG AGAGTATAAA TCTGTAAAAG AAAGAGGAGT ACACATTGTT
    TCAGAGCACT GGCTTTTAGA ATGTGCCCAA GAGTATAAAC ATCTTCCTGA ATCTCTTTAT
    CCACATACTT ATAATCCCAA AATGAGCTTG GATATCAGTA CAGTGCAAGA TGGCAGAATC
    TCTAATAGTC GACTGCTCTC AGCTGATTCA ACGACAAAGG ATGATGAGCC AGATCATTTG
    CCTGGAGAAG AAAACGATAT AGACAATATG ACCACCAGTA ATAAAGAATC CGCAACATCA
    AATGGAGATG GAAGGAATGA CTCTAAAGGA GCTCTGACAC AGACTTTGGA GATGAGAGAG
    AATTTTCAAA AGCAGCTGCA AGAGATAATG TCTGCAACAT CAATAGTGAA ACCTCAGGGA
    CCAAGGGCTT CCCTTTCAAG AAGTGGTTGT AACAGTGCTT CTTCAACCCC TGATAGCACT
    CGCTCTGCCC GCAGTGGGCG AAGTAGAGTC CTGGAGGCAC TGAGGCAGTC TCGTCAAACG
    GTACCTGATA TCAACACAGA GCCCTCCCAG AATGAACAGA TCATTTGGGA TGACCCGACA
    GCCAGGGAAG AGAGAGCAAG GCTTGCCAGC AATCTGCAGT GGCCTAGTTG TCCCACACAG
    TATTCTGAGC TTCAGGTTGA CATTAAAAAA TTGGAGGATT CTCCTTTCCA GGAGCCTTTA
    CATGATCCAG AAATTGCTGA ACAGGTCTGT GATCCCGGAA ATGTGTGTGT GACTGAAGCC
    CCAAAACACC CGACCTCTGA AGAACTGGAA ACACCAGTAA AAGACAGCCA CCTGATCCCT
    ACACCTCAAG CTCCCAGTAT TGCCTTCCCC CTAGCCAACC CACCTGTGGC TCCACACCCT
    AGAGAAAAGA TTATAACAAT AGAGGAAACC CATGAAGACT TGAAAAAACA GTACATATTT
    CAGTTGTCGT CTCTGAATCC TCAAGAACGT ATTGATTATT GTCATCTGAT TGAAAAACTA
    GGTGGATTAG TAATAGAGAA GCAATGCTTT GATCCCAACT GTACACACAT TGTTGTGGGA
    CATCCTCTTC GAAACGAGAA GTATTTAGCT TCAGTGGCAG CTGGGAAGTG GGTACTTCAT
    CGCTCCTACC TGGAAGCATG CCGGACTGCT GGGCACTTCG TGCTGGAAGA AGACTATGAA
    TGGGGAAGTA GTTCCATACT TGATGTTTTG ACTGGAATCA ATGTACAGCA ACGAAGACTA
    GCACTTGCAG CAATGAGATG GAGGAAAAAA ATCCAGCAAA GACAAGAATC AGGCATTGTT
    GAGGGGGCAT TTAGTGGGTG GAAGGTTATC TTACATGTTG ACCAGTCCCG AGAAGCAGGA
    TTCAAACGCC TTCTTCAGTC AGGAGGAGCA AAGGTACTAC CTGGTCATTC TGTACCTTTA
    TTTAAAGAAG CTACACATCT CTTTTCTGAC TTCAATAAAC TGAAACCAGA TGACTCAGGA
    GTTAATATAG CAGAAGCTGC TGCCCAAAAT GTGTACTGCT TGAAAACAGA GTACATTGCT
    GATTATCTGA TGCAGGAATC ACCTCCTCCA GTAGAAAATT ACTGTCTACC AGAAGCAGTT
    TCATTTCTTC AGAATAATAA GGAACCTGGG CCTGGATTAT CACAGAAGAG GAAAGCTCCT
    ACAGAGAAAA ATAAAATCAA ACGACCTAGA GTAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24163
    Clone ID Related Accession (Same CDS sequence) XM_005634446.2 , XM_005634446.3
    Accession Version XM_005634446.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4527bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DNA topoisomerase 2-binding protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005634446.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTCTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    GAATGTTTTT TTAAAGCTCT GGAGTCAATA AAAGAATTTC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA CAGGAAAATG ATAGGTCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGC
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAACGATGTG TCCCAAGAGC TGAACATCCA
    GTCTATAATA TGGTTATGTC TGATGTAACT ATATCTTGTA CAAGCCTGGA AAAAGACAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GTGTATATAG AGATCTTAAT
    ATATCAGTAA CTCATCTTAT TGCAGGAGAA GTTGGCAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCGATTTT GCTTCCTTCT TGGATTAAAA CACTTTGGGA AAAGTCACAA
    GAGAAAAAAA TATCTAGATA TACTGATGTA AACATGGAAG ACTTCAAGTG CCCAATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGGTTG TGTAGCTTAG ATAGGAAGGC AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATATATG GGACAACTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT GTTGACAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ACCAGAAACA AAGACTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCC ACACAGTTGA CAATGTCAGC CATATTTCCA ACATCAATGC AAGTTGCATA
    AATGAATCAA TGTGTAATTC AGTGCTTAAT AGCAAAGTGG AGCCTACCCT TGAAAATCTA
    GAAAATTTGG ATGTCAGTGC ATTTCAAGCA CCTGAAGATT TATTAGATGG TTGTCGGATC
    TATCTTTGCG GTTTTAGTGG CAGAAGGCTA GATAAACTAA GAAGGCTTAT TAACAGTGGA
    GGTGGAGTTC GTTTTAATCA GCTCAATGAA GATGTAACTC ATGTTATAGT GGGAGATTAT
    GATGATGAAT TGAAGCAGTT TTGGGATAAA TCGGCCCACA GGCCTCATGT AGTAGGAGCA
    AAATGGTTGC TGGAGTGTTT TAGTAAAGGT TATATGCTTC CTGAAGAAAC ATATATCCAT
    GCTAACTACC AGCCAGTGGA AATTCCAGTT TCAGATCAAC CTGAAAATAA AACAGCCCTT
    TTAAAAAAGA ACAACAGCTT TTCTAAGAAA GACTTTGCTT CTGATGAAAA GCATGAACAA
    GCTGATGAAG ATCTGCTCTC TCAATATGTA AATAATAACC CGACAGTAGG TGAAGCTGTG
    AAGTCTGAAG TTGAGCCCTT TAATGACTCT ACTCATATTT CTCCGCAAAA AGAAAACCAG
    TCTTCTGCCA CTCATTGCCT CCCTGATGCT TCTACAACTA CTGAAGAAGG TTTATTTAGC
    CAAAAGAGTT TCCTTGTTTT GGGGTTTAGT AATGAAAATG AATCTAATAT TGCAAATATC
    ATAAGAGAAA ATGCTGGAAA AGTCGTGTCC CTTCAGAGCA GAATTGTTGC TGATTATGCT
    GTGGTTCCTC TGCTGGGGTG TGAAGTAGAA GCAACTGTAG GAGAAGTCGT CACAAATACA
    TGGCTGGTAA CTTGTATAGA CTATCAGACT TTAATTGATC CAAAGTCAAA TCCTCTCTTC
    ATGCCAGTTC CATTTATGGC AGGAATGACT CCTTTAGAGG ATTGTGTTAT TTCATTCAGC
    CAGTGTGCAG GAGCAGAAAA AGATTCATTG ACATTCTTAG CAAATCGCCT TGGAGCTAGC
    GTTCAAGAAT TCTTTGTTCG CAAATCCAAT GCAAAGAAAG GCATGTTTGC CAGTACACAT
    CTTGTATTGA AAGAACCAGG TGGTTCAAAA TATGAAGCTG CAAAGAAGTG GAATTTGCCA
    GCAGTCACCA TATCTTGGCT GTTGGAGACT GCTAGAATGG GAAAGAGAGC AAATGAAAAC
    CATTTTCTGA TTGAAAAGTC AACTAAAGAA GAACAAAGTT TGGAAACTGA AATAACAAAC
    AAGGTGAATC TAAATCCAGA TACTCCAGAG CATCCTGTTG CACACCTGGA AACTCAGAGG
    AAAACAGCTG TTACACCTTT AGATATGAAC CGCTTTCAGA GTAGAGCTTT CCATGCTGTG
    ATCTCACAAC ACACCAAACA GGTTTCGACC TCCTCGCCAG TGGGACAGCC ACTTCAGAAG
    GAGCCCTCCT TACATCTGGA CACACCATCA AAGTTTCTGT CCAAGGACAA ACTCTTCAAG
    CCTTCCTTTG ATGTGAAGGA TGCACTTGCA GCCTTGGAAA CTCCAGGAGG TCCCAGCCAG
    CAGAAAAGGA AACTGAGTAC ACCACTTTCA GAAGTCATTG TCAGAAACTT GAAACTTGCT
    TTAGCAAATA GCTCTCGAAA TGCTGTTGCT CTTTCTGCCA GCCCTCGGCT GAAGGATGCC
    CAATCAGAGA AGGATGAAGC TCCAAAGCCA CTTCATAAGG TAGTGGTATG TGTGAGTAAG
    AAACTCAGTA AGAAGCAGAG TGAACTGAAT GGGATCGCAG CCTCTTTAGG AGCAGATTAC
    AGATGGAGTT TTGATGAAAC CGTGACTCAT TTCATTTATC AAGGCCGACC AAATGACACT
    AACCGAGAGT ATAAATCTGT AAAAGAAAGA GGAGTACACA TTGTTTCAGA GCACTGGCTT
    TTAGAATGTG CCCAAGAGTA TAAACATCTT CCTGAATCTC TTTATCCACA TACTTATAAT
    CCCAAAATGA GCTTGGATAT CAGTACAGTG CAAGATGGCA GAATCTCTAA TAGTCGACTG
    CTCTCAGCTG ATTCAACGAC AAAGGATGAT GAGCCAGATC ATTTGCCTGG AGAAGAAAAC
    GATATAGACA ATATGACCAC CAGTAATAAA GAATCCGCAA CATCAAATGG AGATGGAAGG
    AATGACTCTA AAGGAGCTCT GACACAGACT TTGGAGATGA GAGAGAATTT TCAAAAGCAG
    CTGCAAGAGA TAATGTCTGC AACATCAATA GTGAAACCTC AGGGACCAAG GGCTTCCCTT
    TCAAGAAGTG GTTGTAACAG TGCTTCTTCA ACCCCTGATA GCACTCGCTC TGCCCGCAGT
    GGGCGAAGTA GAGTCCTGGA GGCACTGAGG CAGTCTCGTC AAACGGTACC TGATATCAAC
    ACAGAGCCCT CCCAGAATGA ACAGATCATT TGGGATGACC CGACAGCCAG GGAAGAGAGA
    GCAAGGCTTG CCAGCAATCT GCAGTGGCCT AGTTGTCCCA CACAGTATTC TGAGCTTCAG
    GTTGACATTA AAAAATTGGA GGATTCTCCT TTCCAGGAGC CTTTACATGA TCCAGAAATT
    GCTGAACAGG CAGTCTGTGA TCCCGGAAAT GTGTGTGTGA CTGAAGCCCC AAAACACCCG
    ACCTCTGAAG AACTGGAAAC ACCAGTAAAA GACAGCCACC TGATCCCTAC ACCTCAAGCT
    CCCAGTATTG CCTTCCCCCT AGCCAACCCA CCTGTGGCTC CACACCCTAG AGAAAAGATT
    ATAACAATAG AGGAAACCCA TGAAGACTTG AAAAAACAGT ACATATTTCA GTTGTCGTCT
    CTGAATCCTC AAGAACGTAT TGATTATTGT CATCTGATTG AAAAACTAGG TGGATTAGTA
    ATAGAGAAGC AATGCTTTGA TCCCAACTGT ACACACATTG TTGTGGGACA TCCTCTTCGA
    AACGAGAAGT ATTTAGCTTC AGTGGCAGCT GGGAAGTGGG TACTTCATCG CTCCTACCTG
    GAAGCATGCC GGACTGCTGG GCACTTCGTG CTGGAAGAAG ACTATGAATG GGGAAGTAGT
    TCCATACTTG ATGTTTTGAC TGGAATCAAT GTACAGCAAC GAAGACTAGC ACTTGCAGCA
    ATGAGATGGA GGAAAAAAAT CCAGCAAAGA CAAGAATCAG GCATTGTTGA GGGGGCATTT
    AGTGGGTGGA AGGTTATCTT ACATGTTGAC CAGTCCCGAG AAGCAGGATT CAAACGCCTT
    CTTCAGTCAG GAGGAGCAAA GGTACTACCT GGTCATTCTG TACCTTTATT TAAAGAAGCT
    ACACATCTCT TTTCTGACTT CAATAAACTG AAACCAGATG ACTCAGGAGT TAATATAGCA
    GAAGCTGCTG CCCAAAATGT GTACTGCTTG AAAACAGAGT ACATTGCTGA TTATCTGATG
    CAGGAATCAC CTCCTCCAGT AGAAAATTAC TGTCTACCAG AAGCAGTTTC ATTTCTTCAG
    AATAATAAGG AACCTGGGCC TGGATTATCA CAGAAGAGGA AAGCTCCTAC AGAGAAAAAT
    AAAATCAAAC GACCTAGAGT AAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634503.1
    CDS185..4711
    Translation

    Target ORF information:

    RefSeq Version XM_005634446.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005634446.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTCTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    GAATGTTTTT TTAAAGCTCT GGAGTCAATA AAAGAATTTC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA CAGGAAAATG ATAGGTCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGC
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAACGATGTG TCCCAAGAGC TGAACATCCA
    GTCTATAATA TGGTTATGTC TGATGTAACT ATATCTTGTA CAAGCCTGGA AAAAGACAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GTGTATATAG AGATCTTAAT
    ATATCAGTAA CTCATCTTAT TGCAGGAGAA GTTGGCAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCGATTTT GCTTCCTTCT TGGATTAAAA CACTTTGGGA AAAGTCACAA
    GAGAAAAAAA TATCTAGATA TACTGATGTA AACATGGAAG ACTTCAAGTG CCCAATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGGTTG TGTAGCTTAG ATAGGAAGGC AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATATATG GGACAACTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT GTTGACAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ACCAGAAACA AAGACTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCC ACACAGTTGA CAATGTCAGC CATATTTCCA ACATCAATGC AAGTTGCATA
    AATGAATCAA TGTGTAATTC AGTGCTTAAT AGCAAAGTGG AGCCTACCCT TGAAAATCTA
    GAAAATTTGG ATGTCAGTGC ATTTCAAGCA CCTGAAGATT TATTAGATGG TTGTCGGATC
    TATCTTTGCG GTTTTAGTGG CAGAAGGCTA GATAAACTAA GAAGGCTTAT TAACAGTGGA
    GGTGGAGTTC GTTTTAATCA GCTCAATGAA GATGTAACTC ATGTTATAGT GGGAGATTAT
    GATGATGAAT TGAAGCAGTT TTGGGATAAA TCGGCCCACA GGCCTCATGT AGTAGGAGCA
    AAATGGTTGC TGGAGTGTTT TAGTAAAGGT TATATGCTTC CTGAAGAAAC ATATATCCAT
    GCTAACTACC AGCCAGTGGA AATTCCAGTT TCAGATCAAC CTGAAAATAA AACAGCCCTT
    TTAAAAAAGA ACAACAGCTT TTCTAAGAAA GACTTTGCTT CTGATGAAAA GCATGAACAA
    GCTGATGAAG ATCTGCTCTC TCAATATGTA AATAATAACC CGACAGTAGG TGAAGCTGTG
    AAGTCTGAAG TTGAGCCCTT TAATGACTCT ACTCATATTT CTCCGCAAAA AGAAAACCAG
    TCTTCTGCCA CTCATTGCCT CCCTGATGCT TCTACAACTA CTGAAGAAGG TTTATTTAGC
    CAAAAGAGTT TCCTTGTTTT GGGGTTTAGT AATGAAAATG AATCTAATAT TGCAAATATC
    ATAAGAGAAA ATGCTGGAAA AGTCGTGTCC CTTCAGAGCA GAATTGTTGC TGATTATGCT
    GTGGTTCCTC TGCTGGGGTG TGAAGTAGAA GCAACTGTAG GAGAAGTCGT CACAAATACA
    TGGCTGGTAA CTTGTATAGA CTATCAGACT TTAATTGATC CAAAGTCAAA TCCTCTCTTC
    ATGCCAGTTC CATTTATGGC AGGAATGACT CCTTTAGAGG ATTGTGTTAT TTCATTCAGC
    CAGTGTGCAG GAGCAGAAAA AGATTCATTG ACATTCTTAG CAAATCGCCT TGGAGCTAGC
    GTTCAAGAAT TCTTTGTTCG CAAATCCAAT GCAAAGAAAG GCATGTTTGC CAGTACACAT
    CTTGTATTGA AAGAACCAGG TGGTTCAAAA TATGAAGCTG CAAAGAAGTG GAATTTGCCA
    GCAGTCACCA TATCTTGGCT GTTGGAGACT GCTAGAATGG GAAAGAGAGC AAATGAAAAC
    CATTTTCTGA TTGAAAAGTC AACTAAAGAA GAACAAAGTT TGGAAACTGA AATAACAAAC
    AAGGTGAATC TAAATCCAGA TACTCCAGAG CATCCTGTTG CACACCTGGA AACTCAGAGG
    AAAACAGCTG TTACACCTTT AGATATGAAC CGCTTTCAGA GTAGAGCTTT CCATGCTGTG
    ATCTCACAAC ACACCAAACA GGTTTCGACC TCCTCGCCAG TGGGACAGCC ACTTCAGAAG
    GAGCCCTCCT TACATCTGGA CACACCATCA AAGTTTCTGT CCAAGGACAA ACTCTTCAAG
    CCTTCCTTTG ATGTGAAGGA TGCACTTGCA GCCTTGGAAA CTCCAGGAGG TCCCAGCCAG
    CAGAAAAGGA AACTGAGTAC ACCACTTTCA GAAGTCATTG TCAGAAACTT GAAACTTGCT
    TTAGCAAATA GCTCTCGAAA TGCTGTTGCT CTTTCTGCCA GCCCTCGGCT GAAGGATGCC
    CAATCAGAGA AGGATGAAGC TCCAAAGCCA CTTCATAAGG TAGTGGTATG TGTGAGTAAG
    AAACTCAGTA AGAAGCAGAG TGAACTGAAT GGGATCGCAG CCTCTTTAGG AGCAGATTAC
    AGATGGAGTT TTGATGAAAC CGTGACTCAT TTCATTTATC AAGGCCGACC AAATGACACT
    AACCGAGAGT ATAAATCTGT AAAAGAAAGA GGAGTACACA TTGTTTCAGA GCACTGGCTT
    TTAGAATGTG CCCAAGAGTA TAAACATCTT CCTGAATCTC TTTATCCACA TACTTATAAT
    CCCAAAATGA GCTTGGATAT CAGTACAGTG CAAGATGGCA GAATCTCTAA TAGTCGACTG
    CTCTCAGCTG ATTCAACGAC AAAGGATGAT GAGCCAGATC ATTTGCCTGG AGAAGAAAAC
    GATATAGACA ATATGACCAC CAGTAATAAA GAATCCGCAA CATCAAATGG AGATGGAAGG
    AATGACTCTA AAGGAGCTCT GACACAGACT TTGGAGATGA GAGAGAATTT TCAAAAGCAG
    CTGCAAGAGA TAATGTCTGC AACATCAATA GTGAAACCTC AGGGACCAAG GGCTTCCCTT
    TCAAGAAGTG GTTGTAACAG TGCTTCTTCA ACCCCTGATA GCACTCGCTC TGCCCGCAGT
    GGGCGAAGTA GAGTCCTGGA GGCACTGAGG CAGTCTCGTC AAACGGTACC TGATATCAAC
    ACAGAGCCCT CCCAGAATGA ACAGATCATT TGGGATGACC CGACAGCCAG GGAAGAGAGA
    GCAAGGCTTG CCAGCAATCT GCAGTGGCCT AGTTGTCCCA CACAGTATTC TGAGCTTCAG
    GTTGACATTA AAAAATTGGA GGATTCTCCT TTCCAGGAGC CTTTACATGA TCCAGAAATT
    GCTGAACAGG CAGTCTGTGA TCCCGGAAAT GTGTGTGTGA CTGAAGCCCC AAAACACCCG
    ACCTCTGAAG AACTGGAAAC ACCAGTAAAA GACAGCCACC TGATCCCTAC ACCTCAAGCT
    CCCAGTATTG CCTTCCCCCT AGCCAACCCA CCTGTGGCTC CACACCCTAG AGAAAAGATT
    ATAACAATAG AGGAAACCCA TGAAGACTTG AAAAAACAGT ACATATTTCA GTTGTCGTCT
    CTGAATCCTC AAGAACGTAT TGATTATTGT CATCTGATTG AAAAACTAGG TGGATTAGTA
    ATAGAGAAGC AATGCTTTGA TCCCAACTGT ACACACATTG TTGTGGGACA TCCTCTTCGA
    AACGAGAAGT ATTTAGCTTC AGTGGCAGCT GGGAAGTGGG TACTTCATCG CTCCTACCTG
    GAAGCATGCC GGACTGCTGG GCACTTCGTG CTGGAAGAAG ACTATGAATG GGGAAGTAGT
    TCCATACTTG ATGTTTTGAC TGGAATCAAT GTACAGCAAC GAAGACTAGC ACTTGCAGCA
    ATGAGATGGA GGAAAAAAAT CCAGCAAAGA CAAGAATCAG GCATTGTTGA GGGGGCATTT
    AGTGGGTGGA AGGTTATCTT ACATGTTGAC CAGTCCCGAG AAGCAGGATT CAAACGCCTT
    CTTCAGTCAG GAGGAGCAAA GGTACTACCT GGTCATTCTG TACCTTTATT TAAAGAAGCT
    ACACATCTCT TTTCTGACTT CAATAAACTG AAACCAGATG ACTCAGGAGT TAATATAGCA
    GAAGCTGCTG CCCAAAATGT GTACTGCTTG AAAACAGAGT ACATTGCTGA TTATCTGATG
    CAGGAATCAC CTCCTCCAGT AGAAAATTAC TGTCTACCAG AAGCAGTTTC ATTTCTTCAG
    AATAATAAGG AACCTGGGCC TGGATTATCA CAGAAGAGGA AAGCTCCTAC AGAGAAAAAT
    AAAATCAAAC GACCTAGAGT AAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24163
    Clone ID Related Accession (Same CDS sequence) XM_005634446.2 , XM_005634446.3
    Accession Version XM_005634446.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4527bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product DNA topoisomerase 2-binding protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005634446.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTCTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    GAATGTTTTT TTAAAGCTCT GGAGTCAATA AAAGAATTTC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA CAGGAAAATG ATAGGTCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGC
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAACGATGTG TCCCAAGAGC TGAACATCCA
    GTCTATAATA TGGTTATGTC TGATGTAACT ATATCTTGTA CAAGCCTGGA AAAAGACAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GTGTATATAG AGATCTTAAT
    ATATCAGTAA CTCATCTTAT TGCAGGAGAA GTTGGCAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCGATTTT GCTTCCTTCT TGGATTAAAA CACTTTGGGA AAAGTCACAA
    GAGAAAAAAA TATCTAGATA TACTGATGTA AACATGGAAG ACTTCAAGTG CCCAATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGGTTG TGTAGCTTAG ATAGGAAGGC AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATATATG GGACAACTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT GTTGACAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ACCAGAAACA AAGACTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCC ACACAGTTGA CAATGTCAGC CATATTTCCA ACATCAATGC AAGTTGCATA
    AATGAATCAA TGTGTAATTC AGTGCTTAAT AGCAAAGTGG AGCCTACCCT TGAAAATCTA
    GAAAATTTGG ATGTCAGTGC ATTTCAAGCA CCTGAAGATT TATTAGATGG TTGTCGGATC
    TATCTTTGCG GTTTTAGTGG CAGAAGGCTA GATAAACTAA GAAGGCTTAT TAACAGTGGA
    GGTGGAGTTC GTTTTAATCA GCTCAATGAA GATGTAACTC ATGTTATAGT GGGAGATTAT
    GATGATGAAT TGAAGCAGTT TTGGGATAAA TCGGCCCACA GGCCTCATGT AGTAGGAGCA
    AAATGGTTGC TGGAGTGTTT TAGTAAAGGT TATATGCTTC CTGAAGAAAC ATATATCCAT
    GCTAACTACC AGCCAGTGGA AATTCCAGTT TCAGATCAAC CTGAAAATAA AACAGCCCTT
    TTAAAAAAGA ACAACAGCTT TTCTAAGAAA GACTTTGCTT CTGATGAAAA GCATGAACAA
    GCTGATGAAG ATCTGCTCTC TCAATATGTA AATAATAACC CGACAGTAGG TGAAGCTGTG
    AAGTCTGAAG TTGAGCCCTT TAATGACTCT ACTCATATTT CTCCGCAAAA AGAAAACCAG
    TCTTCTGCCA CTCATTGCCT CCCTGATGCT TCTACAACTA CTGAAGAAGG TTTATTTAGC
    CAAAAGAGTT TCCTTGTTTT GGGGTTTAGT AATGAAAATG AATCTAATAT TGCAAATATC
    ATAAGAGAAA ATGCTGGAAA AGTCGTGTCC CTTCAGAGCA GAATTGTTGC TGATTATGCT
    GTGGTTCCTC TGCTGGGGTG TGAAGTAGAA GCAACTGTAG GAGAAGTCGT CACAAATACA
    TGGCTGGTAA CTTGTATAGA CTATCAGACT TTAATTGATC CAAAGTCAAA TCCTCTCTTC
    ATGCCAGTTC CATTTATGGC AGGAATGACT CCTTTAGAGG ATTGTGTTAT TTCATTCAGC
    CAGTGTGCAG GAGCAGAAAA AGATTCATTG ACATTCTTAG CAAATCGCCT TGGAGCTAGC
    GTTCAAGAAT TCTTTGTTCG CAAATCCAAT GCAAAGAAAG GCATGTTTGC CAGTACACAT
    CTTGTATTGA AAGAACCAGG TGGTTCAAAA TATGAAGCTG CAAAGAAGTG GAATTTGCCA
    GCAGTCACCA TATCTTGGCT GTTGGAGACT GCTAGAATGG GAAAGAGAGC AAATGAAAAC
    CATTTTCTGA TTGAAAAGTC AACTAAAGAA GAACAAAGTT TGGAAACTGA AATAACAAAC
    AAGGTGAATC TAAATCCAGA TACTCCAGAG CATCCTGTTG CACACCTGGA AACTCAGAGG
    AAAACAGCTG TTACACCTTT AGATATGAAC CGCTTTCAGA GTAGAGCTTT CCATGCTGTG
    ATCTCACAAC ACACCAAACA GGTTTCGACC TCCTCGCCAG TGGGACAGCC ACTTCAGAAG
    GAGCCCTCCT TACATCTGGA CACACCATCA AAGTTTCTGT CCAAGGACAA ACTCTTCAAG
    CCTTCCTTTG ATGTGAAGGA TGCACTTGCA GCCTTGGAAA CTCCAGGAGG TCCCAGCCAG
    CAGAAAAGGA AACTGAGTAC ACCACTTTCA GAAGTCATTG TCAGAAACTT GAAACTTGCT
    TTAGCAAATA GCTCTCGAAA TGCTGTTGCT CTTTCTGCCA GCCCTCGGCT GAAGGATGCC
    CAATCAGAGA AGGATGAAGC TCCAAAGCCA CTTCATAAGG TAGTGGTATG TGTGAGTAAG
    AAACTCAGTA AGAAGCAGAG TGAACTGAAT GGGATCGCAG CCTCTTTAGG AGCAGATTAC
    AGATGGAGTT TTGATGAAAC CGTGACTCAT TTCATTTATC AAGGCCGACC AAATGACACT
    AACCGAGAGT ATAAATCTGT AAAAGAAAGA GGAGTACACA TTGTTTCAGA GCACTGGCTT
    TTAGAATGTG CCCAAGAGTA TAAACATCTT CCTGAATCTC TTTATCCACA TACTTATAAT
    CCCAAAATGA GCTTGGATAT CAGTACAGTG CAAGATGGCA GAATCTCTAA TAGTCGACTG
    CTCTCAGCTG ATTCAACGAC AAAGGATGAT GAGCCAGATC ATTTGCCTGG AGAAGAAAAC
    GATATAGACA ATATGACCAC CAGTAATAAA GAATCCGCAA CATCAAATGG AGATGGAAGG
    AATGACTCTA AAGGAGCTCT GACACAGACT TTGGAGATGA GAGAGAATTT TCAAAAGCAG
    CTGCAAGAGA TAATGTCTGC AACATCAATA GTGAAACCTC AGGGACCAAG GGCTTCCCTT
    TCAAGAAGTG GTTGTAACAG TGCTTCTTCA ACCCCTGATA GCACTCGCTC TGCCCGCAGT
    GGGCGAAGTA GAGTCCTGGA GGCACTGAGG CAGTCTCGTC AAACGGTACC TGATATCAAC
    ACAGAGCCCT CCCAGAATGA ACAGATCATT TGGGATGACC CGACAGCCAG GGAAGAGAGA
    GCAAGGCTTG CCAGCAATCT GCAGTGGCCT AGTTGTCCCA CACAGTATTC TGAGCTTCAG
    GTTGACATTA AAAAATTGGA GGATTCTCCT TTCCAGGAGC CTTTACATGA TCCAGAAATT
    GCTGAACAGG CAGTCTGTGA TCCCGGAAAT GTGTGTGTGA CTGAAGCCCC AAAACACCCG
    ACCTCTGAAG AACTGGAAAC ACCAGTAAAA GACAGCCACC TGATCCCTAC ACCTCAAGCT
    CCCAGTATTG CCTTCCCCCT AGCCAACCCA CCTGTGGCTC CACACCCTAG AGAAAAGATT
    ATAACAATAG AGGAAACCCA TGAAGACTTG AAAAAACAGT ACATATTTCA GTTGTCGTCT
    CTGAATCCTC AAGAACGTAT TGATTATTGT CATCTGATTG AAAAACTAGG TGGATTAGTA
    ATAGAGAAGC AATGCTTTGA TCCCAACTGT ACACACATTG TTGTGGGACA TCCTCTTCGA
    AACGAGAAGT ATTTAGCTTC AGTGGCAGCT GGGAAGTGGG TACTTCATCG CTCCTACCTG
    GAAGCATGCC GGACTGCTGG GCACTTCGTG CTGGAAGAAG ACTATGAATG GGGAAGTAGT
    TCCATACTTG ATGTTTTGAC TGGAATCAAT GTACAGCAAC GAAGACTAGC ACTTGCAGCA
    ATGAGATGGA GGAAAAAAAT CCAGCAAAGA CAAGAATCAG GCATTGTTGA GGGGGCATTT
    AGTGGGTGGA AGGTTATCTT ACATGTTGAC CAGTCCCGAG AAGCAGGATT CAAACGCCTT
    CTTCAGTCAG GAGGAGCAAA GGTACTACCT GGTCATTCTG TACCTTTATT TAAAGAAGCT
    ACACATCTCT TTTCTGACTT CAATAAACTG AAACCAGATG ACTCAGGAGT TAATATAGCA
    GAAGCTGCTG CCCAAAATGT GTACTGCTTG AAAACAGAGT ACATTGCTGA TTATCTGATG
    CAGGAATCAC CTCCTCCAGT AGAAAATTAC TGTCTACCAG AAGCAGTTTC ATTTCTTCAG
    AATAATAAGG AACCTGGGCC TGGATTATCA CAGAAGAGGA AAGCTCCTAC AGAGAAAAAT
    AAAATCAAAC GACCTAGAGT AAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634503.1
    CDS144..4670
    Translation

    Target ORF information:

    RefSeq Version XM_005634446.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005634446.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTCTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    GAATGTTTTT TTAAAGCTCT GGAGTCAATA AAAGAATTTC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA CAGGAAAATG ATAGGTCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGC
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAACGATGTG TCCCAAGAGC TGAACATCCA
    GTCTATAATA TGGTTATGTC TGATGTAACT ATATCTTGTA CAAGCCTGGA AAAAGACAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GTGTATATAG AGATCTTAAT
    ATATCAGTAA CTCATCTTAT TGCAGGAGAA GTTGGCAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCGATTTT GCTTCCTTCT TGGATTAAAA CACTTTGGGA AAAGTCACAA
    GAGAAAAAAA TATCTAGATA TACTGATGTA AACATGGAAG ACTTCAAGTG CCCAATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGGTTG TGTAGCTTAG ATAGGAAGGC AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATATATG GGACAACTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT GTTGACAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ACCAGAAACA AAGACTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCC ACACAGTTGA CAATGTCAGC CATATTTCCA ACATCAATGC AAGTTGCATA
    AATGAATCAA TGTGTAATTC AGTGCTTAAT AGCAAAGTGG AGCCTACCCT TGAAAATCTA
    GAAAATTTGG ATGTCAGTGC ATTTCAAGCA CCTGAAGATT TATTAGATGG TTGTCGGATC
    TATCTTTGCG GTTTTAGTGG CAGAAGGCTA GATAAACTAA GAAGGCTTAT TAACAGTGGA
    GGTGGAGTTC GTTTTAATCA GCTCAATGAA GATGTAACTC ATGTTATAGT GGGAGATTAT
    GATGATGAAT TGAAGCAGTT TTGGGATAAA TCGGCCCACA GGCCTCATGT AGTAGGAGCA
    AAATGGTTGC TGGAGTGTTT TAGTAAAGGT TATATGCTTC CTGAAGAAAC ATATATCCAT
    GCTAACTACC AGCCAGTGGA AATTCCAGTT TCAGATCAAC CTGAAAATAA AACAGCCCTT
    TTAAAAAAGA ACAACAGCTT TTCTAAGAAA GACTTTGCTT CTGATGAAAA GCATGAACAA
    GCTGATGAAG ATCTGCTCTC TCAATATGTA AATAATAACC CGACAGTAGG TGAAGCTGTG
    AAGTCTGAAG TTGAGCCCTT TAATGACTCT ACTCATATTT CTCCGCAAAA AGAAAACCAG
    TCTTCTGCCA CTCATTGCCT CCCTGATGCT TCTACAACTA CTGAAGAAGG TTTATTTAGC
    CAAAAGAGTT TCCTTGTTTT GGGGTTTAGT AATGAAAATG AATCTAATAT TGCAAATATC
    ATAAGAGAAA ATGCTGGAAA AGTCGTGTCC CTTCAGAGCA GAATTGTTGC TGATTATGCT
    GTGGTTCCTC TGCTGGGGTG TGAAGTAGAA GCAACTGTAG GAGAAGTCGT CACAAATACA
    TGGCTGGTAA CTTGTATAGA CTATCAGACT TTAATTGATC CAAAGTCAAA TCCTCTCTTC
    ATGCCAGTTC CATTTATGGC AGGAATGACT CCTTTAGAGG ATTGTGTTAT TTCATTCAGC
    CAGTGTGCAG GAGCAGAAAA AGATTCATTG ACATTCTTAG CAAATCGCCT TGGAGCTAGC
    GTTCAAGAAT TCTTTGTTCG CAAATCCAAT GCAAAGAAAG GCATGTTTGC CAGTACACAT
    CTTGTATTGA AAGAACCAGG TGGTTCAAAA TATGAAGCTG CAAAGAAGTG GAATTTGCCA
    GCAGTCACCA TATCTTGGCT GTTGGAGACT GCTAGAATGG GAAAGAGAGC AAATGAAAAC
    CATTTTCTGA TTGAAAAGTC AACTAAAGAA GAACAAAGTT TGGAAACTGA AATAACAAAC
    AAGGTGAATC TAAATCCAGA TACTCCAGAG CATCCTGTTG CACACCTGGA AACTCAGAGG
    AAAACAGCTG TTACACCTTT AGATATGAAC CGCTTTCAGA GTAGAGCTTT CCATGCTGTG
    ATCTCACAAC ACACCAAACA GGTTTCGACC TCCTCGCCAG TGGGACAGCC ACTTCAGAAG
    GAGCCCTCCT TACATCTGGA CACACCATCA AAGTTTCTGT CCAAGGACAA ACTCTTCAAG
    CCTTCCTTTG ATGTGAAGGA TGCACTTGCA GCCTTGGAAA CTCCAGGAGG TCCCAGCCAG
    CAGAAAAGGA AACTGAGTAC ACCACTTTCA GAAGTCATTG TCAGAAACTT GAAACTTGCT
    TTAGCAAATA GCTCTCGAAA TGCTGTTGCT CTTTCTGCCA GCCCTCGGCT GAAGGATGCC
    CAATCAGAGA AGGATGAAGC TCCAAAGCCA CTTCATAAGG TAGTGGTATG TGTGAGTAAG
    AAACTCAGTA AGAAGCAGAG TGAACTGAAT GGGATCGCAG CCTCTTTAGG AGCAGATTAC
    AGATGGAGTT TTGATGAAAC CGTGACTCAT TTCATTTATC AAGGCCGACC AAATGACACT
    AACCGAGAGT ATAAATCTGT AAAAGAAAGA GGAGTACACA TTGTTTCAGA GCACTGGCTT
    TTAGAATGTG CCCAAGAGTA TAAACATCTT CCTGAATCTC TTTATCCACA TACTTATAAT
    CCCAAAATGA GCTTGGATAT CAGTACAGTG CAAGATGGCA GAATCTCTAA TAGTCGACTG
    CTCTCAGCTG ATTCAACGAC AAAGGATGAT GAGCCAGATC ATTTGCCTGG AGAAGAAAAC
    GATATAGACA ATATGACCAC CAGTAATAAA GAATCCGCAA CATCAAATGG AGATGGAAGG
    AATGACTCTA AAGGAGCTCT GACACAGACT TTGGAGATGA GAGAGAATTT TCAAAAGCAG
    CTGCAAGAGA TAATGTCTGC AACATCAATA GTGAAACCTC AGGGACCAAG GGCTTCCCTT
    TCAAGAAGTG GTTGTAACAG TGCTTCTTCA ACCCCTGATA GCACTCGCTC TGCCCGCAGT
    GGGCGAAGTA GAGTCCTGGA GGCACTGAGG CAGTCTCGTC AAACGGTACC TGATATCAAC
    ACAGAGCCCT CCCAGAATGA ACAGATCATT TGGGATGACC CGACAGCCAG GGAAGAGAGA
    GCAAGGCTTG CCAGCAATCT GCAGTGGCCT AGTTGTCCCA CACAGTATTC TGAGCTTCAG
    GTTGACATTA AAAAATTGGA GGATTCTCCT TTCCAGGAGC CTTTACATGA TCCAGAAATT
    GCTGAACAGG CAGTCTGTGA TCCCGGAAAT GTGTGTGTGA CTGAAGCCCC AAAACACCCG
    ACCTCTGAAG AACTGGAAAC ACCAGTAAAA GACAGCCACC TGATCCCTAC ACCTCAAGCT
    CCCAGTATTG CCTTCCCCCT AGCCAACCCA CCTGTGGCTC CACACCCTAG AGAAAAGATT
    ATAACAATAG AGGAAACCCA TGAAGACTTG AAAAAACAGT ACATATTTCA GTTGTCGTCT
    CTGAATCCTC AAGAACGTAT TGATTATTGT CATCTGATTG AAAAACTAGG TGGATTAGTA
    ATAGAGAAGC AATGCTTTGA TCCCAACTGT ACACACATTG TTGTGGGACA TCCTCTTCGA
    AACGAGAAGT ATTTAGCTTC AGTGGCAGCT GGGAAGTGGG TACTTCATCG CTCCTACCTG
    GAAGCATGCC GGACTGCTGG GCACTTCGTG CTGGAAGAAG ACTATGAATG GGGAAGTAGT
    TCCATACTTG ATGTTTTGAC TGGAATCAAT GTACAGCAAC GAAGACTAGC ACTTGCAGCA
    ATGAGATGGA GGAAAAAAAT CCAGCAAAGA CAAGAATCAG GCATTGTTGA GGGGGCATTT
    AGTGGGTGGA AGGTTATCTT ACATGTTGAC CAGTCCCGAG AAGCAGGATT CAAACGCCTT
    CTTCAGTCAG GAGGAGCAAA GGTACTACCT GGTCATTCTG TACCTTTATT TAAAGAAGCT
    ACACATCTCT TTTCTGACTT CAATAAACTG AAACCAGATG ACTCAGGAGT TAATATAGCA
    GAAGCTGCTG CCCAAAATGT GTACTGCTTG AAAACAGAGT ACATTGCTGA TTATCTGATG
    CAGGAATCAC CTCCTCCAGT AGAAAATTAC TGTCTACCAG AAGCAGTTTC ATTTCTTCAG
    AATAATAAGG AACCTGGGCC TGGATTATCA CAGAAGAGGA AAGCTCCTAC AGAGAAAAAT
    AAAATCAAAC GACCTAGAGT AAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24164
    Clone ID Related Accession (Same CDS sequence) XM_005634447.2 , XM_005634447.3
    Accession Version XM_005634447.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DNA topoisomerase 2-binding protein 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005634447.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCAGTATC TGCACAATTT ATGGAAGCTT GGCTGCAGAA TTGTTGGCCC TCAAGTAGTC 
    ATATTTTGTA TGCACCACCA ACGATGTGTC CCAAGAGCTG AACATCCAGT CTATAATATG
    GTTATGTCTG ATGTAACTAT ATCTTGTACA AGCCTGGAAA AAGACAAAAG GGAAGAAGTT
    CATAAATATG TACAAATGAT GGGTGGACGT GTATATAGAG ATCTTAATAT ATCAGTAACT
    CATCTTATTG CAGGAGAAGT TGGCAGCAAA AAATACTTAG TTGCTGCAAA CCTAAAGAAA
    CCGATTTTGC TTCCTTCTTG GATTAAAACA CTTTGGGAAA AGTCACAAGA GAAAAAAATA
    TCTAGATATA CTGATGTAAA CATGGAAGAC TTCAAGTGCC CAATTTTTCT TGGTTGCATA
    ATCTGTGTGA CTGGGTTGTG TAGCTTAGAT AGGAAGGCAG TTCAGCAACT CACAGTTAAG
    CATGGAGGTC AATATATGGG ACAACTGAAA ATGAATGAAT GTACACACCT CATTGTGCAA
    GAACCAAAAG GTCAAAAATA TGAATGTGCC AAGAGATGGA ATGTACACTG TGTGACCACA
    CAGTGGTTTT TTGACAGTGT TGACAAAGGT TTTTGTCAGG ATGAATCCAT ATACAAGACA
    GAGCCTAGAC CAGAAACAAA GACTATGCCT GATACTTCAA CTCCTACTGG CCAGATCCAC
    ACAGTTGACA GTCGTACTCT TTCAGATGTC AGCCATATTT CCAACATCAA TGCAAGTTGC
    ATAAATGAAT CAATGTGTAA TTCAGTGCTT AATAGCAAAG TGGAGCCTAC CCTTGAAAAT
    CTAGAAAATT TGGATGTCAG TGCATTTCAA GCACCTGAAG ATTTATTAGA TGGTTGTCGG
    ATCTATCTTT GCGGTTTTAG TGGCAGAAGG CTAGATAAAC TAAGAAGGCT TATTAACAGT
    GGAGGTGGAG TTCGTTTTAA TCAGCTCAAT GAAGATGTAA CTCATGTTAT AGTGGGAGAT
    TATGATGATG AATTGAAGCA GTTTTGGGAT AAATCGGCCC ACAGGCCTCA TGTAGTAGGA
    GCAAAATGGT TGCTGGAGTG TTTTAGTAAA GGTTATATGC TTCCTGAAGA AACATATATC
    CATGCTAACT ACCAGCCAGT GGAAATTCCA GTTTCAGATC AACCTGAAAA TAAAACAGCC
    CTTTTAAAAA AGAACAACAG CTTTTCTAAG AAAGACTTTG CTTCTGATGA AAAGCATGAA
    CAAGCTGATG AAGATCTGCT CTCTCAATAT GTAAATAATA ACCCGACAGT AGGTGAAGCT
    GTGAAGTCTG AAGTTGAGCC CTTTAATGAC TCTACTCATA TTTCTCCGCA AAAAGAAAAC
    CAGTCTTCTG CCACTCATTG CCTCCCTGAT GCTTCTACAA CTACTGAAGA AGGTTTATTT
    AGCCAAAAGA GTTTCCTTGT TTTGGGGTTT AGTAATGAAA ATGAATCTAA TATTGCAAAT
    ATCATAAGAG AAAATGCTGG AAAAGTCGTG TCCCTTCAGA GCAGAATTGT TGCTGATTAT
    GCTGTGGTTC CTCTGCTGGG GTGTGAAGTA GAAGCAACTG TAGGAGAAGT CGTCACAAAT
    ACATGGCTGG TAACTTGTAT AGACTATCAG ACTTTAATTG ATCCAAAGTC AAATCCTCTC
    TTCATGCCAG TTCCATTTAT GGCAGGAATG ACTCCTTTAG AGGATTGTGT TATTTCATTC
    AGCCAGTGTG CAGGAGCAGA AAAAGATTCA TTGACATTCT TAGCAAATCG CCTTGGAGCT
    AGCGTTCAAG AATTCTTTGT TCGCAAATCC AATGCAAAGA AAGGCATGTT TGCCAGTACA
    CATCTTGTAT TGAAAGAACC AGGTGGTTCA AAATATGAAG CTGCAAAGAA GTGGAATTTG
    CCAGCAGTCA CCATATCTTG GCTGTTGGAG ACTGCTAGAA TGGGAAAGAG AGCAAATGAA
    AACCATTTTC TGATTGAAAA GTCAACTAAA GAAGAACAAA GTTTGGAAAC TGAAATAACA
    AACAAGGTGA ATCTAAATCC AGATACTCCA GAGCATCCTG TTGCACACCT GGAAACTCAG
    AGGAAAACAG CTGTTACACC TTTAGATATG AACCGCTTTC AGAGTAGAGC TTTCCATGCT
    GTGATCTCAC AACACACCAA ACAGGTTTCG ACCTCCTCGC CAGTGGGACA GCCACTTCAG
    AAGGAGCCCT CCTTACATCT GGACACACCA TCAAAGTTTC TGTCCAAGGA CAAACTCTTC
    AAGCCTTCCT TTGATGTGAA GGATGCACTT GCAGCCTTGG AAACTCCAGG AGGTCCCAGC
    CAGCAGAAAA GGAAACTGAG TACACCACTT TCAGAAGTCA TTGTCAGAAA CTTGAAACTT
    GCTTTAGCAA ATAGCTCTCG AAATGCTGTT GCTCTTTCTG CCAGCCCTCG GCTGAAGGAT
    GCCCAATCAG AGAAGGATGA AGCTCCAAAG CCACTTCATA AGGTAGTGGT ATGTGTGAGT
    AAGAAACTCA GTAAGAAGCA GAGTGAACTG AATGGGATCG CAGCCTCTTT AGGAGCAGAT
    TACAGATGGA GTTTTGATGA AACCGTGACT CATTTCATTT ATCAAGGCCG ACCAAATGAC
    ACTAACCGAG AGTATAAATC TGTAAAAGAA AGAGGAGTAC ACATTGTTTC AGAGCACTGG
    CTTTTAGAAT GTGCCCAAGA GTATAAACAT CTTCCTGAAT CTCTTTATCC ACATACTTAT
    AATCCCAAAA TGAGCTTGGA TATCAGTACA GTGCAAGATG GCAGAATCTC TAATAGTCGA
    CTGCTCTCAG CTGATTCAAC GACAAAGGAT GATGAGCCAG ATCATTTGCC TGGAGAAGAA
    AACGATATAG ACAATATGAC CACCAGTAAT AAAGAATCCG CAACATCAAA TGGAGATGGA
    AGGAATGACT CTAAAGGAGC TCTGACACAG ACTTTGGAGA TGAGAGAGAA TTTTCAAAAG
    CAGCTGCAAG AGATAATGTC TGCAACATCA ATAGTGAAAC CTCAGGGACC AAGGGCTTCC
    CTTTCAAGAA GTGGTTGTAA CAGTGCTTCT TCAACCCCTG ATAGCACTCG CTCTGCCCGC
    AGTGGGCGAA GTAGAGTCCT GGAGGCACTG AGGCAGTCTC GTCAAACGGT ACCTGATATC
    AACACAGAGC CCTCCCAGAA TGAACAGATC ATTTGGGATG ACCCGACAGC CAGGGAAGAG
    AGAGCAAGGC TTGCCAGCAA TCTGCAGTGG CCTAGTTGTC CCACACAGTA TTCTGAGCTT
    CAGGTTGACA TTAAAAAATT GGAGGATTCT CCTTTCCAGG AGCCTTTACA TGATCCAGAA
    ATTGCTGAAC AGGCAGTCTG TGATCCCGGA AATGTGTGTG TGACTGAAGC CCCAAAACAC
    CCGACCTCTG AAGAACTGGA AACACCAGTA AAAGACAGCC ACCTGATCCC TACACCTCAA
    GCTCCCAGTA TTGCCTTCCC CCTAGCCAAC CCACCTGTGG CTCCACACCC TAGAGAAAAG
    ATTATAACAA TAGAGGAAAC CCATGAAGAC TTGAAAAAAC AGTACATATT TCAGTTGTCG
    TCTCTGAATC CTCAAGAACG TATTGATTAT TGTCATCTGA TTGAAAAACT AGGTGGATTA
    GTAATAGAGA AGCAATGCTT TGATCCCAAC TGTACACACA TTGTTGTGGG ACATCCTCTT
    CGAAACGAGA AGTATTTAGC TTCAGTGGCA GCTGGGAAGT GGGTACTTCA TCGCTCCTAC
    CTGGAAGCAT GCCGGACTGC TGGGCACTTC GTGCTGGAAG AAGACTATGA ATGGGGAAGT
    AGTTCCATAC TTGATGTTTT GACTGGAATC AATGTACAGC AACGAAGACT AGCACTTGCA
    GCAATGAGAT GGAGGAAAAA AATCCAGCAA AGACAAGAAT CAGGCATTGT TGAGGGGGCA
    TTTAGTGGGT GGAAGGTTAT CTTACATGTT GACCAGTCCC GAGAAGCAGG ATTCAAACGC
    CTTCTTCAGT CAGGAGGAGC AAAGGTACTA CCTGGTCATT CTGTACCTTT ATTTAAAGAA
    GCTACACATC TCTTTTCTGA CTTCAATAAA CTGAAACCAG ATGACTCAGG AGTTAATATA
    GCAGAAGCTG CTGCCCAAAA TGTGTACTGC TTGAAAACAG AGTACATTGC TGATTATCTG
    ATGCAGGAAT CACCTCCTCC AGTAGAAAAT TACTGTCTAC CAGAAGCAGT TTCATTTCTT
    CAGAATAATA AGGAACCTGG GCCTGGATTA TCACAGAAGA GGAAAGCTCC TACAGAGAAA
    AATAAAATCA AACGACCTAG AGTAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634504.1
    CDS229..4578
    Translation

    Target ORF information:

    RefSeq Version XM_005634447.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005634447.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCAGTATC TGCACAATTT ATGGAAGCTT GGCTGCAGAA TTGTTGGCCC TCAAGTAGTC 
    ATATTTTGTA TGCACCACCA ACGATGTGTC CCAAGAGCTG AACATCCAGT CTATAATATG
    GTTATGTCTG ATGTAACTAT ATCTTGTACA AGCCTGGAAA AAGACAAAAG GGAAGAAGTT
    CATAAATATG TACAAATGAT GGGTGGACGT GTATATAGAG ATCTTAATAT ATCAGTAACT
    CATCTTATTG CAGGAGAAGT TGGCAGCAAA AAATACTTAG TTGCTGCAAA CCTAAAGAAA
    CCGATTTTGC TTCCTTCTTG GATTAAAACA CTTTGGGAAA AGTCACAAGA GAAAAAAATA
    TCTAGATATA CTGATGTAAA CATGGAAGAC TTCAAGTGCC CAATTTTTCT TGGTTGCATA
    ATCTGTGTGA CTGGGTTGTG TAGCTTAGAT AGGAAGGCAG TTCAGCAACT CACAGTTAAG
    CATGGAGGTC AATATATGGG ACAACTGAAA ATGAATGAAT GTACACACCT CATTGTGCAA
    GAACCAAAAG GTCAAAAATA TGAATGTGCC AAGAGATGGA ATGTACACTG TGTGACCACA
    CAGTGGTTTT TTGACAGTGT TGACAAAGGT TTTTGTCAGG ATGAATCCAT ATACAAGACA
    GAGCCTAGAC CAGAAACAAA GACTATGCCT GATACTTCAA CTCCTACTGG CCAGATCCAC
    ACAGTTGACA GTCGTACTCT TTCAGATGTC AGCCATATTT CCAACATCAA TGCAAGTTGC
    ATAAATGAAT CAATGTGTAA TTCAGTGCTT AATAGCAAAG TGGAGCCTAC CCTTGAAAAT
    CTAGAAAATT TGGATGTCAG TGCATTTCAA GCACCTGAAG ATTTATTAGA TGGTTGTCGG
    ATCTATCTTT GCGGTTTTAG TGGCAGAAGG CTAGATAAAC TAAGAAGGCT TATTAACAGT
    GGAGGTGGAG TTCGTTTTAA TCAGCTCAAT GAAGATGTAA CTCATGTTAT AGTGGGAGAT
    TATGATGATG AATTGAAGCA GTTTTGGGAT AAATCGGCCC ACAGGCCTCA TGTAGTAGGA
    GCAAAATGGT TGCTGGAGTG TTTTAGTAAA GGTTATATGC TTCCTGAAGA AACATATATC
    CATGCTAACT ACCAGCCAGT GGAAATTCCA GTTTCAGATC AACCTGAAAA TAAAACAGCC
    CTTTTAAAAA AGAACAACAG CTTTTCTAAG AAAGACTTTG CTTCTGATGA AAAGCATGAA
    CAAGCTGATG AAGATCTGCT CTCTCAATAT GTAAATAATA ACCCGACAGT AGGTGAAGCT
    GTGAAGTCTG AAGTTGAGCC CTTTAATGAC TCTACTCATA TTTCTCCGCA AAAAGAAAAC
    CAGTCTTCTG CCACTCATTG CCTCCCTGAT GCTTCTACAA CTACTGAAGA AGGTTTATTT
    AGCCAAAAGA GTTTCCTTGT TTTGGGGTTT AGTAATGAAA ATGAATCTAA TATTGCAAAT
    ATCATAAGAG AAAATGCTGG AAAAGTCGTG TCCCTTCAGA GCAGAATTGT TGCTGATTAT
    GCTGTGGTTC CTCTGCTGGG GTGTGAAGTA GAAGCAACTG TAGGAGAAGT CGTCACAAAT
    ACATGGCTGG TAACTTGTAT AGACTATCAG ACTTTAATTG ATCCAAAGTC AAATCCTCTC
    TTCATGCCAG TTCCATTTAT GGCAGGAATG ACTCCTTTAG AGGATTGTGT TATTTCATTC
    AGCCAGTGTG CAGGAGCAGA AAAAGATTCA TTGACATTCT TAGCAAATCG CCTTGGAGCT
    AGCGTTCAAG AATTCTTTGT TCGCAAATCC AATGCAAAGA AAGGCATGTT TGCCAGTACA
    CATCTTGTAT TGAAAGAACC AGGTGGTTCA AAATATGAAG CTGCAAAGAA GTGGAATTTG
    CCAGCAGTCA CCATATCTTG GCTGTTGGAG ACTGCTAGAA TGGGAAAGAG AGCAAATGAA
    AACCATTTTC TGATTGAAAA GTCAACTAAA GAAGAACAAA GTTTGGAAAC TGAAATAACA
    AACAAGGTGA ATCTAAATCC AGATACTCCA GAGCATCCTG TTGCACACCT GGAAACTCAG
    AGGAAAACAG CTGTTACACC TTTAGATATG AACCGCTTTC AGAGTAGAGC TTTCCATGCT
    GTGATCTCAC AACACACCAA ACAGGTTTCG ACCTCCTCGC CAGTGGGACA GCCACTTCAG
    AAGGAGCCCT CCTTACATCT GGACACACCA TCAAAGTTTC TGTCCAAGGA CAAACTCTTC
    AAGCCTTCCT TTGATGTGAA GGATGCACTT GCAGCCTTGG AAACTCCAGG AGGTCCCAGC
    CAGCAGAAAA GGAAACTGAG TACACCACTT TCAGAAGTCA TTGTCAGAAA CTTGAAACTT
    GCTTTAGCAA ATAGCTCTCG AAATGCTGTT GCTCTTTCTG CCAGCCCTCG GCTGAAGGAT
    GCCCAATCAG AGAAGGATGA AGCTCCAAAG CCACTTCATA AGGTAGTGGT ATGTGTGAGT
    AAGAAACTCA GTAAGAAGCA GAGTGAACTG AATGGGATCG CAGCCTCTTT AGGAGCAGAT
    TACAGATGGA GTTTTGATGA AACCGTGACT CATTTCATTT ATCAAGGCCG ACCAAATGAC
    ACTAACCGAG AGTATAAATC TGTAAAAGAA AGAGGAGTAC ACATTGTTTC AGAGCACTGG
    CTTTTAGAAT GTGCCCAAGA GTATAAACAT CTTCCTGAAT CTCTTTATCC ACATACTTAT
    AATCCCAAAA TGAGCTTGGA TATCAGTACA GTGCAAGATG GCAGAATCTC TAATAGTCGA
    CTGCTCTCAG CTGATTCAAC GACAAAGGAT GATGAGCCAG ATCATTTGCC TGGAGAAGAA
    AACGATATAG ACAATATGAC CACCAGTAAT AAAGAATCCG CAACATCAAA TGGAGATGGA
    AGGAATGACT CTAAAGGAGC TCTGACACAG ACTTTGGAGA TGAGAGAGAA TTTTCAAAAG
    CAGCTGCAAG AGATAATGTC TGCAACATCA ATAGTGAAAC CTCAGGGACC AAGGGCTTCC
    CTTTCAAGAA GTGGTTGTAA CAGTGCTTCT TCAACCCCTG ATAGCACTCG CTCTGCCCGC
    AGTGGGCGAA GTAGAGTCCT GGAGGCACTG AGGCAGTCTC GTCAAACGGT ACCTGATATC
    AACACAGAGC CCTCCCAGAA TGAACAGATC ATTTGGGATG ACCCGACAGC CAGGGAAGAG
    AGAGCAAGGC TTGCCAGCAA TCTGCAGTGG CCTAGTTGTC CCACACAGTA TTCTGAGCTT
    CAGGTTGACA TTAAAAAATT GGAGGATTCT CCTTTCCAGG AGCCTTTACA TGATCCAGAA
    ATTGCTGAAC AGGCAGTCTG TGATCCCGGA AATGTGTGTG TGACTGAAGC CCCAAAACAC
    CCGACCTCTG AAGAACTGGA AACACCAGTA AAAGACAGCC ACCTGATCCC TACACCTCAA
    GCTCCCAGTA TTGCCTTCCC CCTAGCCAAC CCACCTGTGG CTCCACACCC TAGAGAAAAG
    ATTATAACAA TAGAGGAAAC CCATGAAGAC TTGAAAAAAC AGTACATATT TCAGTTGTCG
    TCTCTGAATC CTCAAGAACG TATTGATTAT TGTCATCTGA TTGAAAAACT AGGTGGATTA
    GTAATAGAGA AGCAATGCTT TGATCCCAAC TGTACACACA TTGTTGTGGG ACATCCTCTT
    CGAAACGAGA AGTATTTAGC TTCAGTGGCA GCTGGGAAGT GGGTACTTCA TCGCTCCTAC
    CTGGAAGCAT GCCGGACTGC TGGGCACTTC GTGCTGGAAG AAGACTATGA ATGGGGAAGT
    AGTTCCATAC TTGATGTTTT GACTGGAATC AATGTACAGC AACGAAGACT AGCACTTGCA
    GCAATGAGAT GGAGGAAAAA AATCCAGCAA AGACAAGAAT CAGGCATTGT TGAGGGGGCA
    TTTAGTGGGT GGAAGGTTAT CTTACATGTT GACCAGTCCC GAGAAGCAGG ATTCAAACGC
    CTTCTTCAGT CAGGAGGAGC AAAGGTACTA CCTGGTCATT CTGTACCTTT ATTTAAAGAA
    GCTACACATC TCTTTTCTGA CTTCAATAAA CTGAAACCAG ATGACTCAGG AGTTAATATA
    GCAGAAGCTG CTGCCCAAAA TGTGTACTGC TTGAAAACAG AGTACATTGC TGATTATCTG
    ATGCAGGAAT CACCTCCTCC AGTAGAAAAT TACTGTCTAC CAGAAGCAGT TTCATTTCTT
    CAGAATAATA AGGAACCTGG GCCTGGATTA TCACAGAAGA GGAAAGCTCC TACAGAGAAA
    AATAAAATCA AACGACCTAG AGTAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24164
    Clone ID Related Accession (Same CDS sequence) XM_005634447.2 , XM_005634447.3
    Accession Version XM_005634447.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product DNA topoisomerase 2-binding protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005634447.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCAGTATC TGCACAATTT ATGGAAGCTT GGCTGCAGAA TTGTTGGCCC TCAAGTAGTC 
    ATATTTTGTA TGCACCACCA ACGATGTGTC CCAAGAGCTG AACATCCAGT CTATAATATG
    GTTATGTCTG ATGTAACTAT ATCTTGTACA AGCCTGGAAA AAGACAAAAG GGAAGAAGTT
    CATAAATATG TACAAATGAT GGGTGGACGT GTATATAGAG ATCTTAATAT ATCAGTAACT
    CATCTTATTG CAGGAGAAGT TGGCAGCAAA AAATACTTAG TTGCTGCAAA CCTAAAGAAA
    CCGATTTTGC TTCCTTCTTG GATTAAAACA CTTTGGGAAA AGTCACAAGA GAAAAAAATA
    TCTAGATATA CTGATGTAAA CATGGAAGAC TTCAAGTGCC CAATTTTTCT TGGTTGCATA
    ATCTGTGTGA CTGGGTTGTG TAGCTTAGAT AGGAAGGCAG TTCAGCAACT CACAGTTAAG
    CATGGAGGTC AATATATGGG ACAACTGAAA ATGAATGAAT GTACACACCT CATTGTGCAA
    GAACCAAAAG GTCAAAAATA TGAATGTGCC AAGAGATGGA ATGTACACTG TGTGACCACA
    CAGTGGTTTT TTGACAGTGT TGACAAAGGT TTTTGTCAGG ATGAATCCAT ATACAAGACA
    GAGCCTAGAC CAGAAACAAA GACTATGCCT GATACTTCAA CTCCTACTGG CCAGATCCAC
    ACAGTTGACA GTCGTACTCT TTCAGATGTC AGCCATATTT CCAACATCAA TGCAAGTTGC
    ATAAATGAAT CAATGTGTAA TTCAGTGCTT AATAGCAAAG TGGAGCCTAC CCTTGAAAAT
    CTAGAAAATT TGGATGTCAG TGCATTTCAA GCACCTGAAG ATTTATTAGA TGGTTGTCGG
    ATCTATCTTT GCGGTTTTAG TGGCAGAAGG CTAGATAAAC TAAGAAGGCT TATTAACAGT
    GGAGGTGGAG TTCGTTTTAA TCAGCTCAAT GAAGATGTAA CTCATGTTAT AGTGGGAGAT
    TATGATGATG AATTGAAGCA GTTTTGGGAT AAATCGGCCC ACAGGCCTCA TGTAGTAGGA
    GCAAAATGGT TGCTGGAGTG TTTTAGTAAA GGTTATATGC TTCCTGAAGA AACATATATC
    CATGCTAACT ACCAGCCAGT GGAAATTCCA GTTTCAGATC AACCTGAAAA TAAAACAGCC
    CTTTTAAAAA AGAACAACAG CTTTTCTAAG AAAGACTTTG CTTCTGATGA AAAGCATGAA
    CAAGCTGATG AAGATCTGCT CTCTCAATAT GTAAATAATA ACCCGACAGT AGGTGAAGCT
    GTGAAGTCTG AAGTTGAGCC CTTTAATGAC TCTACTCATA TTTCTCCGCA AAAAGAAAAC
    CAGTCTTCTG CCACTCATTG CCTCCCTGAT GCTTCTACAA CTACTGAAGA AGGTTTATTT
    AGCCAAAAGA GTTTCCTTGT TTTGGGGTTT AGTAATGAAA ATGAATCTAA TATTGCAAAT
    ATCATAAGAG AAAATGCTGG AAAAGTCGTG TCCCTTCAGA GCAGAATTGT TGCTGATTAT
    GCTGTGGTTC CTCTGCTGGG GTGTGAAGTA GAAGCAACTG TAGGAGAAGT CGTCACAAAT
    ACATGGCTGG TAACTTGTAT AGACTATCAG ACTTTAATTG ATCCAAAGTC AAATCCTCTC
    TTCATGCCAG TTCCATTTAT GGCAGGAATG ACTCCTTTAG AGGATTGTGT TATTTCATTC
    AGCCAGTGTG CAGGAGCAGA AAAAGATTCA TTGACATTCT TAGCAAATCG CCTTGGAGCT
    AGCGTTCAAG AATTCTTTGT TCGCAAATCC AATGCAAAGA AAGGCATGTT TGCCAGTACA
    CATCTTGTAT TGAAAGAACC AGGTGGTTCA AAATATGAAG CTGCAAAGAA GTGGAATTTG
    CCAGCAGTCA CCATATCTTG GCTGTTGGAG ACTGCTAGAA TGGGAAAGAG AGCAAATGAA
    AACCATTTTC TGATTGAAAA GTCAACTAAA GAAGAACAAA GTTTGGAAAC TGAAATAACA
    AACAAGGTGA ATCTAAATCC AGATACTCCA GAGCATCCTG TTGCACACCT GGAAACTCAG
    AGGAAAACAG CTGTTACACC TTTAGATATG AACCGCTTTC AGAGTAGAGC TTTCCATGCT
    GTGATCTCAC AACACACCAA ACAGGTTTCG ACCTCCTCGC CAGTGGGACA GCCACTTCAG
    AAGGAGCCCT CCTTACATCT GGACACACCA TCAAAGTTTC TGTCCAAGGA CAAACTCTTC
    AAGCCTTCCT TTGATGTGAA GGATGCACTT GCAGCCTTGG AAACTCCAGG AGGTCCCAGC
    CAGCAGAAAA GGAAACTGAG TACACCACTT TCAGAAGTCA TTGTCAGAAA CTTGAAACTT
    GCTTTAGCAA ATAGCTCTCG AAATGCTGTT GCTCTTTCTG CCAGCCCTCG GCTGAAGGAT
    GCCCAATCAG AGAAGGATGA AGCTCCAAAG CCACTTCATA AGGTAGTGGT ATGTGTGAGT
    AAGAAACTCA GTAAGAAGCA GAGTGAACTG AATGGGATCG CAGCCTCTTT AGGAGCAGAT
    TACAGATGGA GTTTTGATGA AACCGTGACT CATTTCATTT ATCAAGGCCG ACCAAATGAC
    ACTAACCGAG AGTATAAATC TGTAAAAGAA AGAGGAGTAC ACATTGTTTC AGAGCACTGG
    CTTTTAGAAT GTGCCCAAGA GTATAAACAT CTTCCTGAAT CTCTTTATCC ACATACTTAT
    AATCCCAAAA TGAGCTTGGA TATCAGTACA GTGCAAGATG GCAGAATCTC TAATAGTCGA
    CTGCTCTCAG CTGATTCAAC GACAAAGGAT GATGAGCCAG ATCATTTGCC TGGAGAAGAA
    AACGATATAG ACAATATGAC CACCAGTAAT AAAGAATCCG CAACATCAAA TGGAGATGGA
    AGGAATGACT CTAAAGGAGC TCTGACACAG ACTTTGGAGA TGAGAGAGAA TTTTCAAAAG
    CAGCTGCAAG AGATAATGTC TGCAACATCA ATAGTGAAAC CTCAGGGACC AAGGGCTTCC
    CTTTCAAGAA GTGGTTGTAA CAGTGCTTCT TCAACCCCTG ATAGCACTCG CTCTGCCCGC
    AGTGGGCGAA GTAGAGTCCT GGAGGCACTG AGGCAGTCTC GTCAAACGGT ACCTGATATC
    AACACAGAGC CCTCCCAGAA TGAACAGATC ATTTGGGATG ACCCGACAGC CAGGGAAGAG
    AGAGCAAGGC TTGCCAGCAA TCTGCAGTGG CCTAGTTGTC CCACACAGTA TTCTGAGCTT
    CAGGTTGACA TTAAAAAATT GGAGGATTCT CCTTTCCAGG AGCCTTTACA TGATCCAGAA
    ATTGCTGAAC AGGCAGTCTG TGATCCCGGA AATGTGTGTG TGACTGAAGC CCCAAAACAC
    CCGACCTCTG AAGAACTGGA AACACCAGTA AAAGACAGCC ACCTGATCCC TACACCTCAA
    GCTCCCAGTA TTGCCTTCCC CCTAGCCAAC CCACCTGTGG CTCCACACCC TAGAGAAAAG
    ATTATAACAA TAGAGGAAAC CCATGAAGAC TTGAAAAAAC AGTACATATT TCAGTTGTCG
    TCTCTGAATC CTCAAGAACG TATTGATTAT TGTCATCTGA TTGAAAAACT AGGTGGATTA
    GTAATAGAGA AGCAATGCTT TGATCCCAAC TGTACACACA TTGTTGTGGG ACATCCTCTT
    CGAAACGAGA AGTATTTAGC TTCAGTGGCA GCTGGGAAGT GGGTACTTCA TCGCTCCTAC
    CTGGAAGCAT GCCGGACTGC TGGGCACTTC GTGCTGGAAG AAGACTATGA ATGGGGAAGT
    AGTTCCATAC TTGATGTTTT GACTGGAATC AATGTACAGC AACGAAGACT AGCACTTGCA
    GCAATGAGAT GGAGGAAAAA AATCCAGCAA AGACAAGAAT CAGGCATTGT TGAGGGGGCA
    TTTAGTGGGT GGAAGGTTAT CTTACATGTT GACCAGTCCC GAGAAGCAGG ATTCAAACGC
    CTTCTTCAGT CAGGAGGAGC AAAGGTACTA CCTGGTCATT CTGTACCTTT ATTTAAAGAA
    GCTACACATC TCTTTTCTGA CTTCAATAAA CTGAAACCAG ATGACTCAGG AGTTAATATA
    GCAGAAGCTG CTGCCCAAAA TGTGTACTGC TTGAAAACAG AGTACATTGC TGATTATCTG
    ATGCAGGAAT CACCTCCTCC AGTAGAAAAT TACTGTCTAC CAGAAGCAGT TTCATTTCTT
    CAGAATAATA AGGAACCTGG GCCTGGATTA TCACAGAAGA GGAAAGCTCC TACAGAGAAA
    AATAAAATCA AACGACCTAG AGTAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634504.1
    CDS276..4625
    Translation

    Target ORF information:

    RefSeq Version XM_005634447.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005634447.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCAGTATC TGCACAATTT ATGGAAGCTT GGCTGCAGAA TTGTTGGCCC TCAAGTAGTC 
    ATATTTTGTA TGCACCACCA ACGATGTGTC CCAAGAGCTG AACATCCAGT CTATAATATG
    GTTATGTCTG ATGTAACTAT ATCTTGTACA AGCCTGGAAA AAGACAAAAG GGAAGAAGTT
    CATAAATATG TACAAATGAT GGGTGGACGT GTATATAGAG ATCTTAATAT ATCAGTAACT
    CATCTTATTG CAGGAGAAGT TGGCAGCAAA AAATACTTAG TTGCTGCAAA CCTAAAGAAA
    CCGATTTTGC TTCCTTCTTG GATTAAAACA CTTTGGGAAA AGTCACAAGA GAAAAAAATA
    TCTAGATATA CTGATGTAAA CATGGAAGAC TTCAAGTGCC CAATTTTTCT TGGTTGCATA
    ATCTGTGTGA CTGGGTTGTG TAGCTTAGAT AGGAAGGCAG TTCAGCAACT CACAGTTAAG
    CATGGAGGTC AATATATGGG ACAACTGAAA ATGAATGAAT GTACACACCT CATTGTGCAA
    GAACCAAAAG GTCAAAAATA TGAATGTGCC AAGAGATGGA ATGTACACTG TGTGACCACA
    CAGTGGTTTT TTGACAGTGT TGACAAAGGT TTTTGTCAGG ATGAATCCAT ATACAAGACA
    GAGCCTAGAC CAGAAACAAA GACTATGCCT GATACTTCAA CTCCTACTGG CCAGATCCAC
    ACAGTTGACA GTCGTACTCT TTCAGATGTC AGCCATATTT CCAACATCAA TGCAAGTTGC
    ATAAATGAAT CAATGTGTAA TTCAGTGCTT AATAGCAAAG TGGAGCCTAC CCTTGAAAAT
    CTAGAAAATT TGGATGTCAG TGCATTTCAA GCACCTGAAG ATTTATTAGA TGGTTGTCGG
    ATCTATCTTT GCGGTTTTAG TGGCAGAAGG CTAGATAAAC TAAGAAGGCT TATTAACAGT
    GGAGGTGGAG TTCGTTTTAA TCAGCTCAAT GAAGATGTAA CTCATGTTAT AGTGGGAGAT
    TATGATGATG AATTGAAGCA GTTTTGGGAT AAATCGGCCC ACAGGCCTCA TGTAGTAGGA
    GCAAAATGGT TGCTGGAGTG TTTTAGTAAA GGTTATATGC TTCCTGAAGA AACATATATC
    CATGCTAACT ACCAGCCAGT GGAAATTCCA GTTTCAGATC AACCTGAAAA TAAAACAGCC
    CTTTTAAAAA AGAACAACAG CTTTTCTAAG AAAGACTTTG CTTCTGATGA AAAGCATGAA
    CAAGCTGATG AAGATCTGCT CTCTCAATAT GTAAATAATA ACCCGACAGT AGGTGAAGCT
    GTGAAGTCTG AAGTTGAGCC CTTTAATGAC TCTACTCATA TTTCTCCGCA AAAAGAAAAC
    CAGTCTTCTG CCACTCATTG CCTCCCTGAT GCTTCTACAA CTACTGAAGA AGGTTTATTT
    AGCCAAAAGA GTTTCCTTGT TTTGGGGTTT AGTAATGAAA ATGAATCTAA TATTGCAAAT
    ATCATAAGAG AAAATGCTGG AAAAGTCGTG TCCCTTCAGA GCAGAATTGT TGCTGATTAT
    GCTGTGGTTC CTCTGCTGGG GTGTGAAGTA GAAGCAACTG TAGGAGAAGT CGTCACAAAT
    ACATGGCTGG TAACTTGTAT AGACTATCAG ACTTTAATTG ATCCAAAGTC AAATCCTCTC
    TTCATGCCAG TTCCATTTAT GGCAGGAATG ACTCCTTTAG AGGATTGTGT TATTTCATTC
    AGCCAGTGTG CAGGAGCAGA AAAAGATTCA TTGACATTCT TAGCAAATCG CCTTGGAGCT
    AGCGTTCAAG AATTCTTTGT TCGCAAATCC AATGCAAAGA AAGGCATGTT TGCCAGTACA
    CATCTTGTAT TGAAAGAACC AGGTGGTTCA AAATATGAAG CTGCAAAGAA GTGGAATTTG
    CCAGCAGTCA CCATATCTTG GCTGTTGGAG ACTGCTAGAA TGGGAAAGAG AGCAAATGAA
    AACCATTTTC TGATTGAAAA GTCAACTAAA GAAGAACAAA GTTTGGAAAC TGAAATAACA
    AACAAGGTGA ATCTAAATCC AGATACTCCA GAGCATCCTG TTGCACACCT GGAAACTCAG
    AGGAAAACAG CTGTTACACC TTTAGATATG AACCGCTTTC AGAGTAGAGC TTTCCATGCT
    GTGATCTCAC AACACACCAA ACAGGTTTCG ACCTCCTCGC CAGTGGGACA GCCACTTCAG
    AAGGAGCCCT CCTTACATCT GGACACACCA TCAAAGTTTC TGTCCAAGGA CAAACTCTTC
    AAGCCTTCCT TTGATGTGAA GGATGCACTT GCAGCCTTGG AAACTCCAGG AGGTCCCAGC
    CAGCAGAAAA GGAAACTGAG TACACCACTT TCAGAAGTCA TTGTCAGAAA CTTGAAACTT
    GCTTTAGCAA ATAGCTCTCG AAATGCTGTT GCTCTTTCTG CCAGCCCTCG GCTGAAGGAT
    GCCCAATCAG AGAAGGATGA AGCTCCAAAG CCACTTCATA AGGTAGTGGT ATGTGTGAGT
    AAGAAACTCA GTAAGAAGCA GAGTGAACTG AATGGGATCG CAGCCTCTTT AGGAGCAGAT
    TACAGATGGA GTTTTGATGA AACCGTGACT CATTTCATTT ATCAAGGCCG ACCAAATGAC
    ACTAACCGAG AGTATAAATC TGTAAAAGAA AGAGGAGTAC ACATTGTTTC AGAGCACTGG
    CTTTTAGAAT GTGCCCAAGA GTATAAACAT CTTCCTGAAT CTCTTTATCC ACATACTTAT
    AATCCCAAAA TGAGCTTGGA TATCAGTACA GTGCAAGATG GCAGAATCTC TAATAGTCGA
    CTGCTCTCAG CTGATTCAAC GACAAAGGAT GATGAGCCAG ATCATTTGCC TGGAGAAGAA
    AACGATATAG ACAATATGAC CACCAGTAAT AAAGAATCCG CAACATCAAA TGGAGATGGA
    AGGAATGACT CTAAAGGAGC TCTGACACAG ACTTTGGAGA TGAGAGAGAA TTTTCAAAAG
    CAGCTGCAAG AGATAATGTC TGCAACATCA ATAGTGAAAC CTCAGGGACC AAGGGCTTCC
    CTTTCAAGAA GTGGTTGTAA CAGTGCTTCT TCAACCCCTG ATAGCACTCG CTCTGCCCGC
    AGTGGGCGAA GTAGAGTCCT GGAGGCACTG AGGCAGTCTC GTCAAACGGT ACCTGATATC
    AACACAGAGC CCTCCCAGAA TGAACAGATC ATTTGGGATG ACCCGACAGC CAGGGAAGAG
    AGAGCAAGGC TTGCCAGCAA TCTGCAGTGG CCTAGTTGTC CCACACAGTA TTCTGAGCTT
    CAGGTTGACA TTAAAAAATT GGAGGATTCT CCTTTCCAGG AGCCTTTACA TGATCCAGAA
    ATTGCTGAAC AGGCAGTCTG TGATCCCGGA AATGTGTGTG TGACTGAAGC CCCAAAACAC
    CCGACCTCTG AAGAACTGGA AACACCAGTA AAAGACAGCC ACCTGATCCC TACACCTCAA
    GCTCCCAGTA TTGCCTTCCC CCTAGCCAAC CCACCTGTGG CTCCACACCC TAGAGAAAAG
    ATTATAACAA TAGAGGAAAC CCATGAAGAC TTGAAAAAAC AGTACATATT TCAGTTGTCG
    TCTCTGAATC CTCAAGAACG TATTGATTAT TGTCATCTGA TTGAAAAACT AGGTGGATTA
    GTAATAGAGA AGCAATGCTT TGATCCCAAC TGTACACACA TTGTTGTGGG ACATCCTCTT
    CGAAACGAGA AGTATTTAGC TTCAGTGGCA GCTGGGAAGT GGGTACTTCA TCGCTCCTAC
    CTGGAAGCAT GCCGGACTGC TGGGCACTTC GTGCTGGAAG AAGACTATGA ATGGGGAAGT
    AGTTCCATAC TTGATGTTTT GACTGGAATC AATGTACAGC AACGAAGACT AGCACTTGCA
    GCAATGAGAT GGAGGAAAAA AATCCAGCAA AGACAAGAAT CAGGCATTGT TGAGGGGGCA
    TTTAGTGGGT GGAAGGTTAT CTTACATGTT GACCAGTCCC GAGAAGCAGG ATTCAAACGC
    CTTCTTCAGT CAGGAGGAGC AAAGGTACTA CCTGGTCATT CTGTACCTTT ATTTAAAGAA
    GCTACACATC TCTTTTCTGA CTTCAATAAA CTGAAACCAG ATGACTCAGG AGTTAATATA
    GCAGAAGCTG CTGCCCAAAA TGTGTACTGC TTGAAAACAG AGTACATTGC TGATTATCTG
    ATGCAGGAAT CACCTCCTCC AGTAGAAAAT TACTGTCTAC CAGAAGCAGT TTCATTTCTT
    CAGAATAATA AGGAACCTGG GCCTGGATTA TCACAGAAGA GGAAAGCTCC TACAGAGAAA
    AATAAAATCA AACGACCTAG AGTAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24165
    Clone ID Related Accession (Same CDS sequence) XM_005634448.1 , XM_005634448.2
    Accession Version XM_005634448.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4281bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DNA topoisomerase 2-binding protein 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCACCACC AACGATGTGT CCCAAGAGCT GAACATCCAG TCTATAATAT GGTTATGTCT 
    GATGTAACTA TATCTTGTAC AAGCCTGGAA AAAGACAAAA GGGAAGAAGT TCATAAATAT
    GTACAAATGA TGGGTGGACG TGTATATAGA GATCTTAATA TATCAGTAAC TCATCTTATT
    GCAGGAGAAG TTGGCAGCAA AAAATACTTA GTTGCTGCAA ACCTAAAGAA ACCGATTTTG
    CTTCCTTCTT GGATTAAAAC ACTTTGGGAA AAGTCACAAG AGAAAAAAAT ATCTAGATAT
    ACTGATGTAA ACATGGAAGA CTTCAAGTGC CCAATTTTTC TTGGTTGCAT AATCTGTGTG
    ACTGGGTTGT GTAGCTTAGA TAGGAAGGCA GTTCAGCAAC TCACAGTTAA GCATGGAGGT
    CAATATATGG GACAACTGAA AATGAATGAA TGTACACACC TCATTGTGCA AGAACCAAAA
    GGTCAAAAAT ATGAATGTGC CAAGAGATGG AATGTACACT GTGTGACCAC ACAGTGGTTT
    TTTGACAGTG TTGACAAAGG TTTTTGTCAG GATGAATCCA TATACAAGAC AGAGCCTAGA
    CCAGAAACAA AGACTATGCC TGATACTTCA ACTCCTACTG GCCAGATCCA CACAGTTGAC
    AGTCGTACTC TTTCAGATGT CAGCCATATT TCCAACATCA ATGCAAGTTG CATAAATGAA
    TCAATGTGTA ATTCAGTGCT TAATAGCAAA GTGGAGCCTA CCCTTGAAAA TCTAGAAAAT
    TTGGATGTCA GTGCATTTCA AGCACCTGAA GATTTATTAG ATGGTTGTCG GATCTATCTT
    TGCGGTTTTA GTGGCAGAAG GCTAGATAAA CTAAGAAGGC TTATTAACAG TGGAGGTGGA
    GTTCGTTTTA ATCAGCTCAA TGAAGATGTA ACTCATGTTA TAGTGGGAGA TTATGATGAT
    GAATTGAAGC AGTTTTGGGA TAAATCGGCC CACAGGCCTC ATGTAGTAGG AGCAAAATGG
    TTGCTGGAGT GTTTTAGTAA AGGTTATATG CTTCCTGAAG AAACATATAT CCATGCTAAC
    TACCAGCCAG TGGAAATTCC AGTTTCAGAT CAACCTGAAA ATAAAACAGC CCTTTTAAAA
    AAGAACAACA GCTTTTCTAA GAAAGACTTT GCTTCTGATG AAAAGCATGA ACAAGCTGAT
    GAAGATCTGC TCTCTCAATA TGTAAATAAT AACCCGACAG TAGGTGAAGC TGTGAAGTCT
    GAAGTTGAGC CCTTTAATGA CTCTACTCAT ATTTCTCCGC AAAAAGAAAA CCAGTCTTCT
    GCCACTCATT GCCTCCCTGA TGCTTCTACA ACTACTGAAG AAGGTTTATT TAGCCAAAAG
    AGTTTCCTTG TTTTGGGGTT TAGTAATGAA AATGAATCTA ATATTGCAAA TATCATAAGA
    GAAAATGCTG GAAAAGTCGT GTCCCTTCAG AGCAGAATTG TTGCTGATTA TGCTGTGGTT
    CCTCTGCTGG GGTGTGAAGT AGAAGCAACT GTAGGAGAAG TCGTCACAAA TACATGGCTG
    GTAACTTGTA TAGACTATCA GACTTTAATT GATCCAAAGT CAAATCCTCT CTTCATGCCA
    GTTCCATTTA TGGCAGGAAT GACTCCTTTA GAGGATTGTG TTATTTCATT CAGCCAGTGT
    GCAGGAGCAG AAAAAGATTC ATTGACATTC TTAGCAAATC GCCTTGGAGC TAGCGTTCAA
    GAATTCTTTG TTCGCAAATC CAATGCAAAG AAAGGCATGT TTGCCAGTAC ACATCTTGTA
    TTGAAAGAAC CAGGTGGTTC AAAATATGAA GCTGCAAAGA AGTGGAATTT GCCAGCAGTC
    ACCATATCTT GGCTGTTGGA GACTGCTAGA ATGGGAAAGA GAGCAAATGA AAACCATTTT
    CTGATTGAAA AGTCAACTAA AGAAGAACAA AGTTTGGAAA CTGAAATAAC AAACAAGGTG
    AATCTAAATC CAGATACTCC AGAGCATCCT GTTGCACACC TGGAAACTCA GAGGAAAACA
    GCTGTTACAC CTTTAGATAT GAACCGCTTT CAGAGTAGAG CTTTCCATGC TGTGATCTCA
    CAACACACCA AACAGGTTTC GACCTCCTCG CCAGTGGGAC AGCCACTTCA GAAGGAGCCC
    TCCTTACATC TGGACACACC ATCAAAGTTT CTGTCCAAGG ACAAACTCTT CAAGCCTTCC
    TTTGATGTGA AGGATGCACT TGCAGCCTTG GAAACTCCAG GAGGTCCCAG CCAGCAGAAA
    AGGAAACTGA GTACACCACT TTCAGAAGTC ATTGTCAGAA ACTTGAAACT TGCTTTAGCA
    AATAGCTCTC GAAATGCTGT TGCTCTTTCT GCCAGCCCTC GGCTGAAGGA TGCCCAATCA
    GAGAAGGATG AAGCTCCAAA GCCACTTCAT AAGGTAGTGG TATGTGTGAG TAAGAAACTC
    AGTAAGAAGC AGAGTGAACT GAATGGGATC GCAGCCTCTT TAGGAGCAGA TTACAGATGG
    AGTTTTGATG AAACCGTGAC TCATTTCATT TATCAAGGCC GACCAAATGA CACTAACCGA
    GAGTATAAAT CTGTAAAAGA AAGAGGAGTA CACATTGTTT CAGAGCACTG GCTTTTAGAA
    TGTGCCCAAG AGTATAAACA TCTTCCTGAA TCTCTTTATC CACATACTTA TAATCCCAAA
    ATGAGCTTGG ATATCAGTAC AGTGCAAGAT GGCAGAATCT CTAATAGTCG ACTGCTCTCA
    GCTGATTCAA CGACAAAGGA TGATGAGCCA GATCATTTGC CTGGAGAAGA AAACGATATA
    GACAATATGA CCACCAGTAA TAAAGAATCC GCAACATCAA ATGGAGATGG AAGGAATGAC
    TCTAAAGGAG CTCTGACACA GACTTTGGAG ATGAGAGAGA ATTTTCAAAA GCAGCTGCAA
    GAGATAATGT CTGCAACATC AATAGTGAAA CCTCAGGGAC CAAGGGCTTC CCTTTCAAGA
    AGTGGTTGTA ACAGTGCTTC TTCAACCCCT GATAGCACTC GCTCTGCCCG CAGTGGGCGA
    AGTAGAGTCC TGGAGGCACT GAGGCAGTCT CGTCAAACGG TACCTGATAT CAACACAGAG
    CCCTCCCAGA ATGAACAGAT CATTTGGGAT GACCCGACAG CCAGGGAAGA GAGAGCAAGG
    CTTGCCAGCA ATCTGCAGTG GCCTAGTTGT CCCACACAGT ATTCTGAGCT TCAGGTTGAC
    ATTAAAAAAT TGGAGGATTC TCCTTTCCAG GAGCCTTTAC ATGATCCAGA AATTGCTGAA
    CAGGCAGTCT GTGATCCCGG AAATGTGTGT GTGACTGAAG CCCCAAAACA CCCGACCTCT
    GAAGAACTGG AAACACCAGT AAAAGACAGC CACCTGATCC CTACACCTCA AGCTCCCAGT
    ATTGCCTTCC CCCTAGCCAA CCCACCTGTG GCTCCACACC CTAGAGAAAA GATTATAACA
    ATAGAGGAAA CCCATGAAGA CTTGAAAAAA CAGTACATAT TTCAGTTGTC GTCTCTGAAT
    CCTCAAGAAC GTATTGATTA TTGTCATCTG ATTGAAAAAC TAGGTGGATT AGTAATAGAG
    AAGCAATGCT TTGATCCCAA CTGTACACAC ATTGTTGTGG GACATCCTCT TCGAAACGAG
    AAGTATTTAG CTTCAGTGGC AGCTGGGAAG TGGGTACTTC ATCGCTCCTA CCTGGAAGCA
    TGCCGGACTG CTGGGCACTT CGTGCTGGAA GAAGACTATG AATGGGGAAG TAGTTCCATA
    CTTGATGTTT TGACTGGAAT CAATGTACAG CAACGAAGAC TAGCACTTGC AGCAATGAGA
    TGGAGGAAAA AAATCCAGCA AAGACAAGAA TCAGGCATTG TTGAGGGGGC ATTTAGTGGG
    TGGAAGGTTA TCTTACATGT TGACCAGTCC CGAGAAGCAG GATTCAAACG CCTTCTTCAG
    TCAGGAGGAG CAAAGGTACT ACCTGGTCAT TCTGTACCTT TATTTAAAGA AGCTACACAT
    CTCTTTTCTG ACTTCAATAA ACTGAAACCA GATGACTCAG GAGTTAATAT AGCAGAAGCT
    GCTGCCCAAA ATGTGTACTG CTTGAAAACA GAGTACATTG CTGATTATCT GATGCAGGAA
    TCACCTCCTC CAGTAGAAAA TTACTGTCTA CCAGAAGCAG TTTCATTTCT TCAGAATAAT
    AAGGAACCTG GGCCTGGATT ATCACAGAAG AGGAAAGCTC CTACAGAGAA AAATAAAATC
    AAACGACCTA GAGTAAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634505.1
    CDS263..4543
    Translation

    Target ORF information:

    RefSeq Version XM_005634448.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005634448.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCACCACC AACGATGTGT CCCAAGAGCT GAACATCCAG TCTATAATAT GGTTATGTCT 
    GATGTAACTA TATCTTGTAC AAGCCTGGAA AAAGACAAAA GGGAAGAAGT TCATAAATAT
    GTACAAATGA TGGGTGGACG TGTATATAGA GATCTTAATA TATCAGTAAC TCATCTTATT
    GCAGGAGAAG TTGGCAGCAA AAAATACTTA GTTGCTGCAA ACCTAAAGAA ACCGATTTTG
    CTTCCTTCTT GGATTAAAAC ACTTTGGGAA AAGTCACAAG AGAAAAAAAT ATCTAGATAT
    ACTGATGTAA ACATGGAAGA CTTCAAGTGC CCAATTTTTC TTGGTTGCAT AATCTGTGTG
    ACTGGGTTGT GTAGCTTAGA TAGGAAGGCA GTTCAGCAAC TCACAGTTAA GCATGGAGGT
    CAATATATGG GACAACTGAA AATGAATGAA TGTACACACC TCATTGTGCA AGAACCAAAA
    GGTCAAAAAT ATGAATGTGC CAAGAGATGG AATGTACACT GTGTGACCAC ACAGTGGTTT
    TTTGACAGTG TTGACAAAGG TTTTTGTCAG GATGAATCCA TATACAAGAC AGAGCCTAGA
    CCAGAAACAA AGACTATGCC TGATACTTCA ACTCCTACTG GCCAGATCCA CACAGTTGAC
    AGTCGTACTC TTTCAGATGT CAGCCATATT TCCAACATCA ATGCAAGTTG CATAAATGAA
    TCAATGTGTA ATTCAGTGCT TAATAGCAAA GTGGAGCCTA CCCTTGAAAA TCTAGAAAAT
    TTGGATGTCA GTGCATTTCA AGCACCTGAA GATTTATTAG ATGGTTGTCG GATCTATCTT
    TGCGGTTTTA GTGGCAGAAG GCTAGATAAA CTAAGAAGGC TTATTAACAG TGGAGGTGGA
    GTTCGTTTTA ATCAGCTCAA TGAAGATGTA ACTCATGTTA TAGTGGGAGA TTATGATGAT
    GAATTGAAGC AGTTTTGGGA TAAATCGGCC CACAGGCCTC ATGTAGTAGG AGCAAAATGG
    TTGCTGGAGT GTTTTAGTAA AGGTTATATG CTTCCTGAAG AAACATATAT CCATGCTAAC
    TACCAGCCAG TGGAAATTCC AGTTTCAGAT CAACCTGAAA ATAAAACAGC CCTTTTAAAA
    AAGAACAACA GCTTTTCTAA GAAAGACTTT GCTTCTGATG AAAAGCATGA ACAAGCTGAT
    GAAGATCTGC TCTCTCAATA TGTAAATAAT AACCCGACAG TAGGTGAAGC TGTGAAGTCT
    GAAGTTGAGC CCTTTAATGA CTCTACTCAT ATTTCTCCGC AAAAAGAAAA CCAGTCTTCT
    GCCACTCATT GCCTCCCTGA TGCTTCTACA ACTACTGAAG AAGGTTTATT TAGCCAAAAG
    AGTTTCCTTG TTTTGGGGTT TAGTAATGAA AATGAATCTA ATATTGCAAA TATCATAAGA
    GAAAATGCTG GAAAAGTCGT GTCCCTTCAG AGCAGAATTG TTGCTGATTA TGCTGTGGTT
    CCTCTGCTGG GGTGTGAAGT AGAAGCAACT GTAGGAGAAG TCGTCACAAA TACATGGCTG
    GTAACTTGTA TAGACTATCA GACTTTAATT GATCCAAAGT CAAATCCTCT CTTCATGCCA
    GTTCCATTTA TGGCAGGAAT GACTCCTTTA GAGGATTGTG TTATTTCATT CAGCCAGTGT
    GCAGGAGCAG AAAAAGATTC ATTGACATTC TTAGCAAATC GCCTTGGAGC TAGCGTTCAA
    GAATTCTTTG TTCGCAAATC CAATGCAAAG AAAGGCATGT TTGCCAGTAC ACATCTTGTA
    TTGAAAGAAC CAGGTGGTTC AAAATATGAA GCTGCAAAGA AGTGGAATTT GCCAGCAGTC
    ACCATATCTT GGCTGTTGGA GACTGCTAGA ATGGGAAAGA GAGCAAATGA AAACCATTTT
    CTGATTGAAA AGTCAACTAA AGAAGAACAA AGTTTGGAAA CTGAAATAAC AAACAAGGTG
    AATCTAAATC CAGATACTCC AGAGCATCCT GTTGCACACC TGGAAACTCA GAGGAAAACA
    GCTGTTACAC CTTTAGATAT GAACCGCTTT CAGAGTAGAG CTTTCCATGC TGTGATCTCA
    CAACACACCA AACAGGTTTC GACCTCCTCG CCAGTGGGAC AGCCACTTCA GAAGGAGCCC
    TCCTTACATC TGGACACACC ATCAAAGTTT CTGTCCAAGG ACAAACTCTT CAAGCCTTCC
    TTTGATGTGA AGGATGCACT TGCAGCCTTG GAAACTCCAG GAGGTCCCAG CCAGCAGAAA
    AGGAAACTGA GTACACCACT TTCAGAAGTC ATTGTCAGAA ACTTGAAACT TGCTTTAGCA
    AATAGCTCTC GAAATGCTGT TGCTCTTTCT GCCAGCCCTC GGCTGAAGGA TGCCCAATCA
    GAGAAGGATG AAGCTCCAAA GCCACTTCAT AAGGTAGTGG TATGTGTGAG TAAGAAACTC
    AGTAAGAAGC AGAGTGAACT GAATGGGATC GCAGCCTCTT TAGGAGCAGA TTACAGATGG
    AGTTTTGATG AAACCGTGAC TCATTTCATT TATCAAGGCC GACCAAATGA CACTAACCGA
    GAGTATAAAT CTGTAAAAGA AAGAGGAGTA CACATTGTTT CAGAGCACTG GCTTTTAGAA
    TGTGCCCAAG AGTATAAACA TCTTCCTGAA TCTCTTTATC CACATACTTA TAATCCCAAA
    ATGAGCTTGG ATATCAGTAC AGTGCAAGAT GGCAGAATCT CTAATAGTCG ACTGCTCTCA
    GCTGATTCAA CGACAAAGGA TGATGAGCCA GATCATTTGC CTGGAGAAGA AAACGATATA
    GACAATATGA CCACCAGTAA TAAAGAATCC GCAACATCAA ATGGAGATGG AAGGAATGAC
    TCTAAAGGAG CTCTGACACA GACTTTGGAG ATGAGAGAGA ATTTTCAAAA GCAGCTGCAA
    GAGATAATGT CTGCAACATC AATAGTGAAA CCTCAGGGAC CAAGGGCTTC CCTTTCAAGA
    AGTGGTTGTA ACAGTGCTTC TTCAACCCCT GATAGCACTC GCTCTGCCCG CAGTGGGCGA
    AGTAGAGTCC TGGAGGCACT GAGGCAGTCT CGTCAAACGG TACCTGATAT CAACACAGAG
    CCCTCCCAGA ATGAACAGAT CATTTGGGAT GACCCGACAG CCAGGGAAGA GAGAGCAAGG
    CTTGCCAGCA ATCTGCAGTG GCCTAGTTGT CCCACACAGT ATTCTGAGCT TCAGGTTGAC
    ATTAAAAAAT TGGAGGATTC TCCTTTCCAG GAGCCTTTAC ATGATCCAGA AATTGCTGAA
    CAGGCAGTCT GTGATCCCGG AAATGTGTGT GTGACTGAAG CCCCAAAACA CCCGACCTCT
    GAAGAACTGG AAACACCAGT AAAAGACAGC CACCTGATCC CTACACCTCA AGCTCCCAGT
    ATTGCCTTCC CCCTAGCCAA CCCACCTGTG GCTCCACACC CTAGAGAAAA GATTATAACA
    ATAGAGGAAA CCCATGAAGA CTTGAAAAAA CAGTACATAT TTCAGTTGTC GTCTCTGAAT
    CCTCAAGAAC GTATTGATTA TTGTCATCTG ATTGAAAAAC TAGGTGGATT AGTAATAGAG
    AAGCAATGCT TTGATCCCAA CTGTACACAC ATTGTTGTGG GACATCCTCT TCGAAACGAG
    AAGTATTTAG CTTCAGTGGC AGCTGGGAAG TGGGTACTTC ATCGCTCCTA CCTGGAAGCA
    TGCCGGACTG CTGGGCACTT CGTGCTGGAA GAAGACTATG AATGGGGAAG TAGTTCCATA
    CTTGATGTTT TGACTGGAAT CAATGTACAG CAACGAAGAC TAGCACTTGC AGCAATGAGA
    TGGAGGAAAA AAATCCAGCA AAGACAAGAA TCAGGCATTG TTGAGGGGGC ATTTAGTGGG
    TGGAAGGTTA TCTTACATGT TGACCAGTCC CGAGAAGCAG GATTCAAACG CCTTCTTCAG
    TCAGGAGGAG CAAAGGTACT ACCTGGTCAT TCTGTACCTT TATTTAAAGA AGCTACACAT
    CTCTTTTCTG ACTTCAATAA ACTGAAACCA GATGACTCAG GAGTTAATAT AGCAGAAGCT
    GCTGCCCAAA ATGTGTACTG CTTGAAAACA GAGTACATTG CTGATTATCT GATGCAGGAA
    TCACCTCCTC CAGTAGAAAA TTACTGTCTA CCAGAAGCAG TTTCATTTCT TCAGAATAAT
    AAGGAACCTG GGCCTGGATT ATCACAGAAG AGGAAAGCTC CTACAGAGAA AAATAAAATC
    AAACGACCTA GAGTAAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24165
    Clone ID Related Accession (Same CDS sequence) XM_005634448.1 , XM_005634448.2
    Accession Version XM_005634448.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4281bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product DNA topoisomerase 2-binding protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005634448.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCACCACC AACGATGTGT CCCAAGAGCT GAACATCCAG TCTATAATAT GGTTATGTCT 
    GATGTAACTA TATCTTGTAC AAGCCTGGAA AAAGACAAAA GGGAAGAAGT TCATAAATAT
    GTACAAATGA TGGGTGGACG TGTATATAGA GATCTTAATA TATCAGTAAC TCATCTTATT
    GCAGGAGAAG TTGGCAGCAA AAAATACTTA GTTGCTGCAA ACCTAAAGAA ACCGATTTTG
    CTTCCTTCTT GGATTAAAAC ACTTTGGGAA AAGTCACAAG AGAAAAAAAT ATCTAGATAT
    ACTGATGTAA ACATGGAAGA CTTCAAGTGC CCAATTTTTC TTGGTTGCAT AATCTGTGTG
    ACTGGGTTGT GTAGCTTAGA TAGGAAGGCA GTTCAGCAAC TCACAGTTAA GCATGGAGGT
    CAATATATGG GACAACTGAA AATGAATGAA TGTACACACC TCATTGTGCA AGAACCAAAA
    GGTCAAAAAT ATGAATGTGC CAAGAGATGG AATGTACACT GTGTGACCAC ACAGTGGTTT
    TTTGACAGTG TTGACAAAGG TTTTTGTCAG GATGAATCCA TATACAAGAC AGAGCCTAGA
    CCAGAAACAA AGACTATGCC TGATACTTCA ACTCCTACTG GCCAGATCCA CACAGTTGAC
    AGTCGTACTC TTTCAGATGT CAGCCATATT TCCAACATCA ATGCAAGTTG CATAAATGAA
    TCAATGTGTA ATTCAGTGCT TAATAGCAAA GTGGAGCCTA CCCTTGAAAA TCTAGAAAAT
    TTGGATGTCA GTGCATTTCA AGCACCTGAA GATTTATTAG ATGGTTGTCG GATCTATCTT
    TGCGGTTTTA GTGGCAGAAG GCTAGATAAA CTAAGAAGGC TTATTAACAG TGGAGGTGGA
    GTTCGTTTTA ATCAGCTCAA TGAAGATGTA ACTCATGTTA TAGTGGGAGA TTATGATGAT
    GAATTGAAGC AGTTTTGGGA TAAATCGGCC CACAGGCCTC ATGTAGTAGG AGCAAAATGG
    TTGCTGGAGT GTTTTAGTAA AGGTTATATG CTTCCTGAAG AAACATATAT CCATGCTAAC
    TACCAGCCAG TGGAAATTCC AGTTTCAGAT CAACCTGAAA ATAAAACAGC CCTTTTAAAA
    AAGAACAACA GCTTTTCTAA GAAAGACTTT GCTTCTGATG AAAAGCATGA ACAAGCTGAT
    GAAGATCTGC TCTCTCAATA TGTAAATAAT AACCCGACAG TAGGTGAAGC TGTGAAGTCT
    GAAGTTGAGC CCTTTAATGA CTCTACTCAT ATTTCTCCGC AAAAAGAAAA CCAGTCTTCT
    GCCACTCATT GCCTCCCTGA TGCTTCTACA ACTACTGAAG AAGGTTTATT TAGCCAAAAG
    AGTTTCCTTG TTTTGGGGTT TAGTAATGAA AATGAATCTA ATATTGCAAA TATCATAAGA
    GAAAATGCTG GAAAAGTCGT GTCCCTTCAG AGCAGAATTG TTGCTGATTA TGCTGTGGTT
    CCTCTGCTGG GGTGTGAAGT AGAAGCAACT GTAGGAGAAG TCGTCACAAA TACATGGCTG
    GTAACTTGTA TAGACTATCA GACTTTAATT GATCCAAAGT CAAATCCTCT CTTCATGCCA
    GTTCCATTTA TGGCAGGAAT GACTCCTTTA GAGGATTGTG TTATTTCATT CAGCCAGTGT
    GCAGGAGCAG AAAAAGATTC ATTGACATTC TTAGCAAATC GCCTTGGAGC TAGCGTTCAA
    GAATTCTTTG TTCGCAAATC CAATGCAAAG AAAGGCATGT TTGCCAGTAC ACATCTTGTA
    TTGAAAGAAC CAGGTGGTTC AAAATATGAA GCTGCAAAGA AGTGGAATTT GCCAGCAGTC
    ACCATATCTT GGCTGTTGGA GACTGCTAGA ATGGGAAAGA GAGCAAATGA AAACCATTTT
    CTGATTGAAA AGTCAACTAA AGAAGAACAA AGTTTGGAAA CTGAAATAAC AAACAAGGTG
    AATCTAAATC CAGATACTCC AGAGCATCCT GTTGCACACC TGGAAACTCA GAGGAAAACA
    GCTGTTACAC CTTTAGATAT GAACCGCTTT CAGAGTAGAG CTTTCCATGC TGTGATCTCA
    CAACACACCA AACAGGTTTC GACCTCCTCG CCAGTGGGAC AGCCACTTCA GAAGGAGCCC
    TCCTTACATC TGGACACACC ATCAAAGTTT CTGTCCAAGG ACAAACTCTT CAAGCCTTCC
    TTTGATGTGA AGGATGCACT TGCAGCCTTG GAAACTCCAG GAGGTCCCAG CCAGCAGAAA
    AGGAAACTGA GTACACCACT TTCAGAAGTC ATTGTCAGAA ACTTGAAACT TGCTTTAGCA
    AATAGCTCTC GAAATGCTGT TGCTCTTTCT GCCAGCCCTC GGCTGAAGGA TGCCCAATCA
    GAGAAGGATG AAGCTCCAAA GCCACTTCAT AAGGTAGTGG TATGTGTGAG TAAGAAACTC
    AGTAAGAAGC AGAGTGAACT GAATGGGATC GCAGCCTCTT TAGGAGCAGA TTACAGATGG
    AGTTTTGATG AAACCGTGAC TCATTTCATT TATCAAGGCC GACCAAATGA CACTAACCGA
    GAGTATAAAT CTGTAAAAGA AAGAGGAGTA CACATTGTTT CAGAGCACTG GCTTTTAGAA
    TGTGCCCAAG AGTATAAACA TCTTCCTGAA TCTCTTTATC CACATACTTA TAATCCCAAA
    ATGAGCTTGG ATATCAGTAC AGTGCAAGAT GGCAGAATCT CTAATAGTCG ACTGCTCTCA
    GCTGATTCAA CGACAAAGGA TGATGAGCCA GATCATTTGC CTGGAGAAGA AAACGATATA
    GACAATATGA CCACCAGTAA TAAAGAATCC GCAACATCAA ATGGAGATGG AAGGAATGAC
    TCTAAAGGAG CTCTGACACA GACTTTGGAG ATGAGAGAGA ATTTTCAAAA GCAGCTGCAA
    GAGATAATGT CTGCAACATC AATAGTGAAA CCTCAGGGAC CAAGGGCTTC CCTTTCAAGA
    AGTGGTTGTA ACAGTGCTTC TTCAACCCCT GATAGCACTC GCTCTGCCCG CAGTGGGCGA
    AGTAGAGTCC TGGAGGCACT GAGGCAGTCT CGTCAAACGG TACCTGATAT CAACACAGAG
    CCCTCCCAGA ATGAACAGAT CATTTGGGAT GACCCGACAG CCAGGGAAGA GAGAGCAAGG
    CTTGCCAGCA ATCTGCAGTG GCCTAGTTGT CCCACACAGT ATTCTGAGCT TCAGGTTGAC
    ATTAAAAAAT TGGAGGATTC TCCTTTCCAG GAGCCTTTAC ATGATCCAGA AATTGCTGAA
    CAGGCAGTCT GTGATCCCGG AAATGTGTGT GTGACTGAAG CCCCAAAACA CCCGACCTCT
    GAAGAACTGG AAACACCAGT AAAAGACAGC CACCTGATCC CTACACCTCA AGCTCCCAGT
    ATTGCCTTCC CCCTAGCCAA CCCACCTGTG GCTCCACACC CTAGAGAAAA GATTATAACA
    ATAGAGGAAA CCCATGAAGA CTTGAAAAAA CAGTACATAT TTCAGTTGTC GTCTCTGAAT
    CCTCAAGAAC GTATTGATTA TTGTCATCTG ATTGAAAAAC TAGGTGGATT AGTAATAGAG
    AAGCAATGCT TTGATCCCAA CTGTACACAC ATTGTTGTGG GACATCCTCT TCGAAACGAG
    AAGTATTTAG CTTCAGTGGC AGCTGGGAAG TGGGTACTTC ATCGCTCCTA CCTGGAAGCA
    TGCCGGACTG CTGGGCACTT CGTGCTGGAA GAAGACTATG AATGGGGAAG TAGTTCCATA
    CTTGATGTTT TGACTGGAAT CAATGTACAG CAACGAAGAC TAGCACTTGC AGCAATGAGA
    TGGAGGAAAA AAATCCAGCA AAGACAAGAA TCAGGCATTG TTGAGGGGGC ATTTAGTGGG
    TGGAAGGTTA TCTTACATGT TGACCAGTCC CGAGAAGCAG GATTCAAACG CCTTCTTCAG
    TCAGGAGGAG CAAAGGTACT ACCTGGTCAT TCTGTACCTT TATTTAAAGA AGCTACACAT
    CTCTTTTCTG ACTTCAATAA ACTGAAACCA GATGACTCAG GAGTTAATAT AGCAGAAGCT
    GCTGCCCAAA ATGTGTACTG CTTGAAAACA GAGTACATTG CTGATTATCT GATGCAGGAA
    TCACCTCCTC CAGTAGAAAA TTACTGTCTA CCAGAAGCAG TTTCATTTCT TCAGAATAAT
    AAGGAACCTG GGCCTGGATT ATCACAGAAG AGGAAAGCTC CTACAGAGAA AAATAAAATC
    AAACGACCTA GAGTAAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634505.1
    CDS310..4590
    Translation

    Target ORF information:

    RefSeq Version XM_005634448.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005634448.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCACCACC AACGATGTGT CCCAAGAGCT GAACATCCAG TCTATAATAT GGTTATGTCT 
    GATGTAACTA TATCTTGTAC AAGCCTGGAA AAAGACAAAA GGGAAGAAGT TCATAAATAT
    GTACAAATGA TGGGTGGACG TGTATATAGA GATCTTAATA TATCAGTAAC TCATCTTATT
    GCAGGAGAAG TTGGCAGCAA AAAATACTTA GTTGCTGCAA ACCTAAAGAA ACCGATTTTG
    CTTCCTTCTT GGATTAAAAC ACTTTGGGAA AAGTCACAAG AGAAAAAAAT ATCTAGATAT
    ACTGATGTAA ACATGGAAGA CTTCAAGTGC CCAATTTTTC TTGGTTGCAT AATCTGTGTG
    ACTGGGTTGT GTAGCTTAGA TAGGAAGGCA GTTCAGCAAC TCACAGTTAA GCATGGAGGT
    CAATATATGG GACAACTGAA AATGAATGAA TGTACACACC TCATTGTGCA AGAACCAAAA
    GGTCAAAAAT ATGAATGTGC CAAGAGATGG AATGTACACT GTGTGACCAC ACAGTGGTTT
    TTTGACAGTG TTGACAAAGG TTTTTGTCAG GATGAATCCA TATACAAGAC AGAGCCTAGA
    CCAGAAACAA AGACTATGCC TGATACTTCA ACTCCTACTG GCCAGATCCA CACAGTTGAC
    AGTCGTACTC TTTCAGATGT CAGCCATATT TCCAACATCA ATGCAAGTTG CATAAATGAA
    TCAATGTGTA ATTCAGTGCT TAATAGCAAA GTGGAGCCTA CCCTTGAAAA TCTAGAAAAT
    TTGGATGTCA GTGCATTTCA AGCACCTGAA GATTTATTAG ATGGTTGTCG GATCTATCTT
    TGCGGTTTTA GTGGCAGAAG GCTAGATAAA CTAAGAAGGC TTATTAACAG TGGAGGTGGA
    GTTCGTTTTA ATCAGCTCAA TGAAGATGTA ACTCATGTTA TAGTGGGAGA TTATGATGAT
    GAATTGAAGC AGTTTTGGGA TAAATCGGCC CACAGGCCTC ATGTAGTAGG AGCAAAATGG
    TTGCTGGAGT GTTTTAGTAA AGGTTATATG CTTCCTGAAG AAACATATAT CCATGCTAAC
    TACCAGCCAG TGGAAATTCC AGTTTCAGAT CAACCTGAAA ATAAAACAGC CCTTTTAAAA
    AAGAACAACA GCTTTTCTAA GAAAGACTTT GCTTCTGATG AAAAGCATGA ACAAGCTGAT
    GAAGATCTGC TCTCTCAATA TGTAAATAAT AACCCGACAG TAGGTGAAGC TGTGAAGTCT
    GAAGTTGAGC CCTTTAATGA CTCTACTCAT ATTTCTCCGC AAAAAGAAAA CCAGTCTTCT
    GCCACTCATT GCCTCCCTGA TGCTTCTACA ACTACTGAAG AAGGTTTATT TAGCCAAAAG
    AGTTTCCTTG TTTTGGGGTT TAGTAATGAA AATGAATCTA ATATTGCAAA TATCATAAGA
    GAAAATGCTG GAAAAGTCGT GTCCCTTCAG AGCAGAATTG TTGCTGATTA TGCTGTGGTT
    CCTCTGCTGG GGTGTGAAGT AGAAGCAACT GTAGGAGAAG TCGTCACAAA TACATGGCTG
    GTAACTTGTA TAGACTATCA GACTTTAATT GATCCAAAGT CAAATCCTCT CTTCATGCCA
    GTTCCATTTA TGGCAGGAAT GACTCCTTTA GAGGATTGTG TTATTTCATT CAGCCAGTGT
    GCAGGAGCAG AAAAAGATTC ATTGACATTC TTAGCAAATC GCCTTGGAGC TAGCGTTCAA
    GAATTCTTTG TTCGCAAATC CAATGCAAAG AAAGGCATGT TTGCCAGTAC ACATCTTGTA
    TTGAAAGAAC CAGGTGGTTC AAAATATGAA GCTGCAAAGA AGTGGAATTT GCCAGCAGTC
    ACCATATCTT GGCTGTTGGA GACTGCTAGA ATGGGAAAGA GAGCAAATGA AAACCATTTT
    CTGATTGAAA AGTCAACTAA AGAAGAACAA AGTTTGGAAA CTGAAATAAC AAACAAGGTG
    AATCTAAATC CAGATACTCC AGAGCATCCT GTTGCACACC TGGAAACTCA GAGGAAAACA
    GCTGTTACAC CTTTAGATAT GAACCGCTTT CAGAGTAGAG CTTTCCATGC TGTGATCTCA
    CAACACACCA AACAGGTTTC GACCTCCTCG CCAGTGGGAC AGCCACTTCA GAAGGAGCCC
    TCCTTACATC TGGACACACC ATCAAAGTTT CTGTCCAAGG ACAAACTCTT CAAGCCTTCC
    TTTGATGTGA AGGATGCACT TGCAGCCTTG GAAACTCCAG GAGGTCCCAG CCAGCAGAAA
    AGGAAACTGA GTACACCACT TTCAGAAGTC ATTGTCAGAA ACTTGAAACT TGCTTTAGCA
    AATAGCTCTC GAAATGCTGT TGCTCTTTCT GCCAGCCCTC GGCTGAAGGA TGCCCAATCA
    GAGAAGGATG AAGCTCCAAA GCCACTTCAT AAGGTAGTGG TATGTGTGAG TAAGAAACTC
    AGTAAGAAGC AGAGTGAACT GAATGGGATC GCAGCCTCTT TAGGAGCAGA TTACAGATGG
    AGTTTTGATG AAACCGTGAC TCATTTCATT TATCAAGGCC GACCAAATGA CACTAACCGA
    GAGTATAAAT CTGTAAAAGA AAGAGGAGTA CACATTGTTT CAGAGCACTG GCTTTTAGAA
    TGTGCCCAAG AGTATAAACA TCTTCCTGAA TCTCTTTATC CACATACTTA TAATCCCAAA
    ATGAGCTTGG ATATCAGTAC AGTGCAAGAT GGCAGAATCT CTAATAGTCG ACTGCTCTCA
    GCTGATTCAA CGACAAAGGA TGATGAGCCA GATCATTTGC CTGGAGAAGA AAACGATATA
    GACAATATGA CCACCAGTAA TAAAGAATCC GCAACATCAA ATGGAGATGG AAGGAATGAC
    TCTAAAGGAG CTCTGACACA GACTTTGGAG ATGAGAGAGA ATTTTCAAAA GCAGCTGCAA
    GAGATAATGT CTGCAACATC AATAGTGAAA CCTCAGGGAC CAAGGGCTTC CCTTTCAAGA
    AGTGGTTGTA ACAGTGCTTC TTCAACCCCT GATAGCACTC GCTCTGCCCG CAGTGGGCGA
    AGTAGAGTCC TGGAGGCACT GAGGCAGTCT CGTCAAACGG TACCTGATAT CAACACAGAG
    CCCTCCCAGA ATGAACAGAT CATTTGGGAT GACCCGACAG CCAGGGAAGA GAGAGCAAGG
    CTTGCCAGCA ATCTGCAGTG GCCTAGTTGT CCCACACAGT ATTCTGAGCT TCAGGTTGAC
    ATTAAAAAAT TGGAGGATTC TCCTTTCCAG GAGCCTTTAC ATGATCCAGA AATTGCTGAA
    CAGGCAGTCT GTGATCCCGG AAATGTGTGT GTGACTGAAG CCCCAAAACA CCCGACCTCT
    GAAGAACTGG AAACACCAGT AAAAGACAGC CACCTGATCC CTACACCTCA AGCTCCCAGT
    ATTGCCTTCC CCCTAGCCAA CCCACCTGTG GCTCCACACC CTAGAGAAAA GATTATAACA
    ATAGAGGAAA CCCATGAAGA CTTGAAAAAA CAGTACATAT TTCAGTTGTC GTCTCTGAAT
    CCTCAAGAAC GTATTGATTA TTGTCATCTG ATTGAAAAAC TAGGTGGATT AGTAATAGAG
    AAGCAATGCT TTGATCCCAA CTGTACACAC ATTGTTGTGG GACATCCTCT TCGAAACGAG
    AAGTATTTAG CTTCAGTGGC AGCTGGGAAG TGGGTACTTC ATCGCTCCTA CCTGGAAGCA
    TGCCGGACTG CTGGGCACTT CGTGCTGGAA GAAGACTATG AATGGGGAAG TAGTTCCATA
    CTTGATGTTT TGACTGGAAT CAATGTACAG CAACGAAGAC TAGCACTTGC AGCAATGAGA
    TGGAGGAAAA AAATCCAGCA AAGACAAGAA TCAGGCATTG TTGAGGGGGC ATTTAGTGGG
    TGGAAGGTTA TCTTACATGT TGACCAGTCC CGAGAAGCAG GATTCAAACG CCTTCTTCAG
    TCAGGAGGAG CAAAGGTACT ACCTGGTCAT TCTGTACCTT TATTTAAAGA AGCTACACAT
    CTCTTTTCTG ACTTCAATAA ACTGAAACCA GATGACTCAG GAGTTAATAT AGCAGAAGCT
    GCTGCCCAAA ATGTGTACTG CTTGAAAACA GAGTACATTG CTGATTATCT GATGCAGGAA
    TCACCTCCTC CAGTAGAAAA TTACTGTCTA CCAGAAGCAG TTTCATTTCT TCAGAATAAT
    AAGGAACCTG GGCCTGGATT ATCACAGAAG AGGAAAGCTC CTACAGAGAA AAATAAAATC
    AAACGACCTA GAGTAAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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