TOPBP1 cDNA ORF clone, Bos taurus(cattle)

The following TOPBP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOPBP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa218888 XM_024991343.1
Latest version!
Bos taurus DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa87818 XM_015473526.1
Latest version!
Bos taurus topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa16054 NM_001206766.2
Latest version!
Bos taurus DNA topoisomerase II binding protein 1 (TOPBP1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa16052 XM_005201960.4
Latest version!
Bos taurus DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa16053 XM_005201961.3
Latest version!
Bos taurus topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa16053 XM_024991344.1
Latest version!
Bos taurus DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa16052 XM_005201960.3 Bos taurus topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa16054 NM_001206766.1 Bos taurus DNA topoisomerase II binding protein 1 (TOPBP1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa218888
    Clone ID Related Accession (Same CDS sequence) XM_024991343.1
    Accession Version XM_024991343.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-binding protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCCAAAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCT 
    GAATGTTTTT TTGAAGCTCT TGAGTCAATA AAAGAGTTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAAATA AAGGAGAATG ATAGATCACT GTATATCTGT
    GACCCCTTTA GTGGTGATGC CTTTGATCAT CTCAAAAAGC TTGGTTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC TGAACATCCA
    GTTTATAACA TGGTTATGTC TGATGTAACC GTGTCCTGTA CAAGCCTGGA AAAAGACAGA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GCGTATACAG AGACCTTAAC
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAG
    GAGAAGAAAA TAGCTAGATA TACTGATATA AACATGGAAG ACTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTACCTTAG ACAGGAAATC AGTTCAGCAG
    CTAACAGTAA AGCATGGAGG ACAGTACATG GGACAGTTGA AAATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CGCAGTGGTT TTTTGACAGT GTTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ATCAGAAGCA AAGAGTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGCT GCATAAATGA ATCAATATGT AATTCAGTTA ATAGCAAACT GGAACCTACA
    ATTGAAAATC TGGAAAATTT GGATATCAGT GCATTTCAGG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TCTATCTTTG TGGTTTTAAT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAT CAGCTCAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATG ATGATGATGA ATTGAAGCAG TTTTGGGATA AATCAGCCCA CAGGCCGCAT
    GTAGTGGGAG CAAAATGGTT GCTAGAGTGT TTTAGTAAAG GCTACATGCT TCCTGAAGAG
    CCATACATCC ACGTTAACTA CCAGCCAGTG GAAATTCCAG TTTCAGATCC ACCTGAAAGT
    AAAACAGCCC TTTTGAAAAG GAAGAACAAC GGCTTCTCTA AGAGTGACTT TGTTCCTGAG
    GAGAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAGT ATCAGACTAA TAACCCCACA
    GTAGTTGAAG TTGCCAAGTC TGAAGTTGGG CTCTGTAGTG ACTCTACTCA TGTGGAGCCC
    CGTAATGATT CTACTCATAT TTCTCTGCAA GAACACCAGT CTTCCATCAG TCATTGCCTC
    CCTGATGTTT CTACAATTAC CGAAGAAGGT TTATTTAGCC AAAAGAGTTT CCTAGTTTTG
    GGGTTTAGTA ATGAAAATGA ATCTAATATT GCAATCATCA TAAGAGAAAA TGCTGGAAAA
    ATTGTGTCCC TTCAGAGCAG AGTTGTTGCT GATTATGCTG TGGTTCCTCT GCTGGGATGT
    GAAGTACAGG CAACTGTGGG AGAAGTTGTA ACAAACACAT GGCTGGTAAT GTGTATAGAC
    TACCAGACTT TAATTGATCC AAAGTCAAAT CCTCTCTTCA CACCAGTTCC AGTTATGGCA
    GGAATGACTC CTTTAGAGGG TTGTGTTCTT TCATTCAGCC AATGTGTAGG AGCTGAAAAA
    GATTCATTGA CATTCTTAGC AAATCACCTT GGAGCCAGTG TTCAAGAATA CTTCGTTCGC
    AAGTCCAATG CAAAGAAAGG CATGTTTGCT AGTACACATC TTGTTTTGAA AGAGCCAGGT
    GGTTCTAAAT ATGAAGCTGC CAAGAAGTGG AATTTACCAG CAGTCACCAT AGCTTGGCTG
    TTAGAGTCTG CTAGATTGGG AAAGAGAGCA AATGAAAACC ATTTCCTGAT TGAAAATTCA
    TCTAAAGAAG AACAAAGTTT GGAAACTGAA ACAACAAATG AGGTGAATCT AAATCCAGAT
    ACTCCAGAGC ATCCTGTAAC ACATCTGGAA ACTCAGAGAA AAACAGCCGT TACACCTTTA
    GATATGAACC GCTTTCAGAG TAAAGCTTTC CGCACGGTGA TGTCACAGCA CTCCGGACAG
    GCTTCAGTGT CCCCTTCCCC AGGACAGTCA CTTCAGAAGG AGCCCTCATT ACACCTGGAT
    ACGCCGTCAA AGTTTCTGTC CAAGGACAAA CTGTTCAAGC CTTCCTTTGA TGTGAAGGAA
    GATGCTCCGA AGCCACTTGA GAACGTTGTG GTGTGTGTTA GTAAGAAACT CAGTAAGAAG
    CAGAGTGAAC TAAATGGGAT TGCGGCCTCT CTAGGTGCAG ACTACAGGTG GAGTTTTGAT
    GAGACAGTGA CTCATTTCAT CTATCAAGGA CGACCAAATG ACACTAATCG GGAGTATAAA
    TCTGTAAAAG AAAGAGGAGT ACACATTGTT TCAGAGCACT GGCTTTTAGA GTGTGCCCAG
    GAGTATAAAC ATCTTCCCGA ATCTCTTTAT CCACATACTT ACAATCCCAA AATGAGCTTG
    GACATCAGTG CAGTGCAAGA TGGCCGACTC TGTAATAACC GACTACTTTC AGCTGGCTCT
    GCAATGAGGG ATGATGAGCC AGATCATATG CCTCTAGAAG AAAATGATAT AGACAATATG
    ACCACCAGTA ATAAAGAACT AACAACATCA AATGGAAATG GGAGGAATGA CTCTAAAGGA
    GCTCTGACAC AGACTTTGGA GATGAGAGAG AACTTTCAAA AGCAGCTTCA GGAGATAATG
    TCTGCAACTT CAATAGTGAA ACCCCAAGGG CAGAGGACTT CCCTCTCAAG AAGTGGTTGT
    AACAGTACCT CTTCAACTCC TGACAGCACC CGCTCTGCCC GCAGTGGACG AAGTAGAGTC
    CTAGAGGCAC TGAGGCAGTC TCGTCAAACA GTACCTGATG TCAACACAGA GCCCTCCCAA
    AATGAACAGA TCATTTGGGA TGACCCTACA GCCAGGGAGG AGAGAGCAAG ACTTGCCAGC
    AATTTGCAGT GGCCTAGTTG TCCCACACAA TACTCTGAGC TTCAGGTTGA TGTTAAAAAA
    CTGGAGGATT CTCCTTTCCA GGAGCCTTTA CATGATTCAG AAATTGCTGA ACAGGCTGTC
    TGTGATCCTG GGAACGTATG TGTGACCGGA GCCCCAAAAC ACCCGATCTC TGAAGAACTG
    GAAGCTCCAG TGAAAGACAG CCACCTGATC CCTACGCCTC AAGCCCCCAG TATTGCCTTC
    CCCCTAGCCA ACCCACCTGT GGCTCCACAC CCTAGAGAAA AGATTATAAC AATAGAGGAG
    ACTCATGAAG ACTTAAAAAA ACAGTACATA TTTCAGTTGT CATCTCTGAA TCCTCAAGAA
    CGTATTGATT ATTGTCATCT GATTGAAAAA CTAGGTGGAT CAGTGATTGA GAAGCAGTGC
    TTTGATCCCA GCTGTACACA CATTGTTGTG GGGCATCCAC TTCGCAACGA GAAGTATTTA
    GCCTCCGTGG CAGCGGGAAA GTGGGTACTC CATCGCTCCT ACCTCGAAGC TTGCAGGACT
    GCTGGGCGCT TCATGTCGGA AGAAGACTAT GAGTGGGGAA GTAGTTCCAT ACTTGATGTT
    TTGACTGGAA TCAATGTACA GCAACGAAAA CTAGCACTTG CAGCAATGAG ATGGAGGAAA
    AAAATCCAGC AAAGACAAGA ATCAGGCATT ACTGAGGGGG CATTTAGCGG GTGGAAAGTT
    ATTTTACATG TTGATCAGTC CCGAGAATCA GGATTCAAAC GCCTACTTCA GTCAGGAGGA
    GCAAAGGTGC TGCCTGGTTA TTCTGTATCT TTATTTAAAG AAGCTACACA TCTCTTTTCT
    GACTTTAATA AACAGAAACC AGATGACTCA GGAGTTAATA TAGCAGAAGC TGCTGCCCAG
    AATGTATACT GCTTGAAAAC AGAATACATT GCTGATTTTC TTATGCAGGA ATCCCCTCCT
    CAAGTAGAAA ATTACTGTCT ACCAGAAGCT GCTTCATTTC TTCAGAATAA AAAGGAACCT
    GGGCCTGGAT TCTCACAAAA GAGGAAAGCT CCTGCAGAAA AAAATAAAAT CAAACGACCC
    AGAGTACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847111.1
    CDS184..4575
    Translation

    Target ORF information:

    RefSeq Version XM_024991343.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991343.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCCAAAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCT 
    GAATGTTTTT TTGAAGCTCT TGAGTCAATA AAAGAGTTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAAATA AAGGAGAATG ATAGATCACT GTATATCTGT
    GACCCCTTTA GTGGTGATGC CTTTGATCAT CTCAAAAAGC TTGGTTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC TGAACATCCA
    GTTTATAACA TGGTTATGTC TGATGTAACC GTGTCCTGTA CAAGCCTGGA AAAAGACAGA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GCGTATACAG AGACCTTAAC
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAG
    GAGAAGAAAA TAGCTAGATA TACTGATATA AACATGGAAG ACTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTACCTTAG ACAGGAAATC AGTTCAGCAG
    CTAACAGTAA AGCATGGAGG ACAGTACATG GGACAGTTGA AAATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CGCAGTGGTT TTTTGACAGT GTTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ATCAGAAGCA AAGAGTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGCT GCATAAATGA ATCAATATGT AATTCAGTTA ATAGCAAACT GGAACCTACA
    ATTGAAAATC TGGAAAATTT GGATATCAGT GCATTTCAGG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TCTATCTTTG TGGTTTTAAT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAT CAGCTCAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATG ATGATGATGA ATTGAAGCAG TTTTGGGATA AATCAGCCCA CAGGCCGCAT
    GTAGTGGGAG CAAAATGGTT GCTAGAGTGT TTTAGTAAAG GCTACATGCT TCCTGAAGAG
    CCATACATCC ACGTTAACTA CCAGCCAGTG GAAATTCCAG TTTCAGATCC ACCTGAAAGT
    AAAACAGCCC TTTTGAAAAG GAAGAACAAC GGCTTCTCTA AGAGTGACTT TGTTCCTGAG
    GAGAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAGT ATCAGACTAA TAACCCCACA
    GTAGTTGAAG TTGCCAAGTC TGAAGTTGGG CTCTGTAGTG ACTCTACTCA TGTGGAGCCC
    CGTAATGATT CTACTCATAT TTCTCTGCAA GAACACCAGT CTTCCATCAG TCATTGCCTC
    CCTGATGTTT CTACAATTAC CGAAGAAGGT TTATTTAGCC AAAAGAGTTT CCTAGTTTTG
    GGGTTTAGTA ATGAAAATGA ATCTAATATT GCAATCATCA TAAGAGAAAA TGCTGGAAAA
    ATTGTGTCCC TTCAGAGCAG AGTTGTTGCT GATTATGCTG TGGTTCCTCT GCTGGGATGT
    GAAGTACAGG CAACTGTGGG AGAAGTTGTA ACAAACACAT GGCTGGTAAT GTGTATAGAC
    TACCAGACTT TAATTGATCC AAAGTCAAAT CCTCTCTTCA CACCAGTTCC AGTTATGGCA
    GGAATGACTC CTTTAGAGGG TTGTGTTCTT TCATTCAGCC AATGTGTAGG AGCTGAAAAA
    GATTCATTGA CATTCTTAGC AAATCACCTT GGAGCCAGTG TTCAAGAATA CTTCGTTCGC
    AAGTCCAATG CAAAGAAAGG CATGTTTGCT AGTACACATC TTGTTTTGAA AGAGCCAGGT
    GGTTCTAAAT ATGAAGCTGC CAAGAAGTGG AATTTACCAG CAGTCACCAT AGCTTGGCTG
    TTAGAGTCTG CTAGATTGGG AAAGAGAGCA AATGAAAACC ATTTCCTGAT TGAAAATTCA
    TCTAAAGAAG AACAAAGTTT GGAAACTGAA ACAACAAATG AGGTGAATCT AAATCCAGAT
    ACTCCAGAGC ATCCTGTAAC ACATCTGGAA ACTCAGAGAA AAACAGCCGT TACACCTTTA
    GATATGAACC GCTTTCAGAG TAAAGCTTTC CGCACGGTGA TGTCACAGCA CTCCGGACAG
    GCTTCAGTGT CCCCTTCCCC AGGACAGTCA CTTCAGAAGG AGCCCTCATT ACACCTGGAT
    ACGCCGTCAA AGTTTCTGTC CAAGGACAAA CTGTTCAAGC CTTCCTTTGA TGTGAAGGAA
    GATGCTCCGA AGCCACTTGA GAACGTTGTG GTGTGTGTTA GTAAGAAACT CAGTAAGAAG
    CAGAGTGAAC TAAATGGGAT TGCGGCCTCT CTAGGTGCAG ACTACAGGTG GAGTTTTGAT
    GAGACAGTGA CTCATTTCAT CTATCAAGGA CGACCAAATG ACACTAATCG GGAGTATAAA
    TCTGTAAAAG AAAGAGGAGT ACACATTGTT TCAGAGCACT GGCTTTTAGA GTGTGCCCAG
    GAGTATAAAC ATCTTCCCGA ATCTCTTTAT CCACATACTT ACAATCCCAA AATGAGCTTG
    GACATCAGTG CAGTGCAAGA TGGCCGACTC TGTAATAACC GACTACTTTC AGCTGGCTCT
    GCAATGAGGG ATGATGAGCC AGATCATATG CCTCTAGAAG AAAATGATAT AGACAATATG
    ACCACCAGTA ATAAAGAACT AACAACATCA AATGGAAATG GGAGGAATGA CTCTAAAGGA
    GCTCTGACAC AGACTTTGGA GATGAGAGAG AACTTTCAAA AGCAGCTTCA GGAGATAATG
    TCTGCAACTT CAATAGTGAA ACCCCAAGGG CAGAGGACTT CCCTCTCAAG AAGTGGTTGT
    AACAGTACCT CTTCAACTCC TGACAGCACC CGCTCTGCCC GCAGTGGACG AAGTAGAGTC
    CTAGAGGCAC TGAGGCAGTC TCGTCAAACA GTACCTGATG TCAACACAGA GCCCTCCCAA
    AATGAACAGA TCATTTGGGA TGACCCTACA GCCAGGGAGG AGAGAGCAAG ACTTGCCAGC
    AATTTGCAGT GGCCTAGTTG TCCCACACAA TACTCTGAGC TTCAGGTTGA TGTTAAAAAA
    CTGGAGGATT CTCCTTTCCA GGAGCCTTTA CATGATTCAG AAATTGCTGA ACAGGCTGTC
    TGTGATCCTG GGAACGTATG TGTGACCGGA GCCCCAAAAC ACCCGATCTC TGAAGAACTG
    GAAGCTCCAG TGAAAGACAG CCACCTGATC CCTACGCCTC AAGCCCCCAG TATTGCCTTC
    CCCCTAGCCA ACCCACCTGT GGCTCCACAC CCTAGAGAAA AGATTATAAC AATAGAGGAG
    ACTCATGAAG ACTTAAAAAA ACAGTACATA TTTCAGTTGT CATCTCTGAA TCCTCAAGAA
    CGTATTGATT ATTGTCATCT GATTGAAAAA CTAGGTGGAT CAGTGATTGA GAAGCAGTGC
    TTTGATCCCA GCTGTACACA CATTGTTGTG GGGCATCCAC TTCGCAACGA GAAGTATTTA
    GCCTCCGTGG CAGCGGGAAA GTGGGTACTC CATCGCTCCT ACCTCGAAGC TTGCAGGACT
    GCTGGGCGCT TCATGTCGGA AGAAGACTAT GAGTGGGGAA GTAGTTCCAT ACTTGATGTT
    TTGACTGGAA TCAATGTACA GCAACGAAAA CTAGCACTTG CAGCAATGAG ATGGAGGAAA
    AAAATCCAGC AAAGACAAGA ATCAGGCATT ACTGAGGGGG CATTTAGCGG GTGGAAAGTT
    ATTTTACATG TTGATCAGTC CCGAGAATCA GGATTCAAAC GCCTACTTCA GTCAGGAGGA
    GCAAAGGTGC TGCCTGGTTA TTCTGTATCT TTATTTAAAG AAGCTACACA TCTCTTTTCT
    GACTTTAATA AACAGAAACC AGATGACTCA GGAGTTAATA TAGCAGAAGC TGCTGCCCAG
    AATGTATACT GCTTGAAAAC AGAATACATT GCTGATTTTC TTATGCAGGA ATCCCCTCCT
    CAAGTAGAAA ATTACTGTCT ACCAGAAGCT GCTTCATTTC TTCAGAATAA AAAGGAACCT
    GGGCCTGGAT TCTCACAAAA GAGGAAAGCT CCTGCAGAAA AAAATAAAAT CAAACGACCC
    AGAGTACAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87818
    Clone ID Related Accession (Same CDS sequence) XM_015473526.1
    Accession Version XM_015473526.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-binding protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103822.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCCAAAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCT 
    GAATGTTTTT TTGAAGCTCT TGAGTCAATA AAAGAGTTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAAATA AAGGAGAATG ATAGATCACT GTATATCTGT
    GACCCCTTTA GTGGTGATGC CTTTGATCAT CTCAAAAAGC TTGGTTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC TGAACATCCA
    GTTTATAACA TGGTTATGTC TGATGTAACC GTGTCCTGTA CAAGCCTGGA AAAAGACAGA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GCGTATACAG AGACCTTAAC
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAG
    GAGAAGAAAA TAGCTAGATA TACTGATATA AACATGGAAG ACTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTACCTTAG ACAGGAAATC AGTTCAGCAG
    CTAACAGTAA AGCATGGAGG ACAGTACATG GGACAGTTGA AAATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CGCAGTGGTT TTTTGACAGT GTTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ATCAGAAGCA AAGAGTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGCT GCATAAATGA ATCAATATGT AATTCAGTTA ATAGCAAACT GGAACCTACA
    ATTGAAAATC TGGAAAATTT GGATATCAGT GCATTTCAGG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TCTATCTTTG TGGTTTTAAT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAT CAGCTCAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATG ATGATGATGA ATTGAAGCAG TTTTGGGATA AATCAGCCCA CAGGCCGCAT
    GTAGTGGGAG CAAAATGGTT GCTAGAGTGT TTTAGTAAAG GCTACATGCT TCCTGAAGAG
    CCATACATCC ACGTTAACTA CCAGCCAGTG GAAATTCCAG TTTCAGATCC ACCTGAAAGT
    AAAACAGCCC TTTTGAAAAG GAAGAACAAC GGCTTCTCTA AGAGTGACTT TGTTCCTGAG
    GAGAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAGT ATCAGACTAA TAACCCCACA
    GTAGTTGAAG TTGCCAAGTC TGAAGTTGGG CTCTGTAGTG ACTCTACTCA TGTGGAGCCC
    CGTAATGATT CTACTCATAT TTCTCTGCAA GAACACCAGT CTTCCATCAG TCATTGCCTC
    CCTGATGTTT CTACAATTAC CGAAGAAGGT TTATTTAGCC AAAAGAGTTT CCTAGTTTTG
    GGGTTTAGTA ATGAAAATGA ATCTAATATT GCAATCATCA TAAGAGAAAA TGCTGGAAAA
    ATTGTGTCCC TTCAGAGCAG AGTTGTTGCT GATTATGCTG TGGTTCCTCT GCTGGGATGT
    GAAGTACAGG CAACTGTGGG AGAAGTTGTA ACAAACACAT GGCTGGTAAT GTGTATAGAC
    TACCAGACTT TAATTGATCC AAAGTCAAAT CCTCTCTTCA CACCAGTTCC AGTTATGGCA
    GGAATGACTC CTTTAGAGGG TTGTGTTCTT TCATTCAGCC AATGTGTAGG AGCTGAAAAA
    GATTCATTGA CATTCTTAGC AAATCACCTT GGAGCCAGTG TTCAAGAATA CTTCGTTCGC
    AAGTCCAATG CAAAGAAAGG CATGTTTGCT AGTACACATC TTGTTTTGAA AGAGCCAGGT
    GGTTCTAAAT ATGAAGCTGC CAAGAAGTGG AATTTACCAG CAGTCACCAT AGCTTGGCTG
    TTAGAGTCTG CTAGATTGGG AAAGAGAGCA AATGAAAACC ATTTCCTGAT TGAAAATTCA
    TCTAAAGAAG AACAAAGTTT GGAAACTGAA ACAACAAATG AGGTGAATCT AAATCCAGAT
    ACTCCAGAGC ATCCTGTAAC ACATCTGGAA ACTCAGAGAA AAACAGCCGT TACACCTTTA
    GATATGAACC GCTTTCAGAG TAAAGCTTTC CGCACGGTGA TGTCACAGCA CTCCGGACAG
    GCTTCAGTGT CCCCTTCCCC AGGACAGTCA CTTCAGAAGG AGCCCTCATT ACACCTGGAT
    ACGCCGTCAA AGTTTCTGTC CAAGGACAAA CTGTTCAAGC CTTCCTTTGA TGTGAAGGAT
    GCTCTCGCAG CCTTGGAAAC TCCAGGAGGT CCCAGCCAGC AGAAAAGGAA ACTGAGTACA
    CCACTCTCAG AAGTCATCGT CAAAAACCTG AAACTTGCTT TGGCAAATAG CTCTCGAAAT
    GCTGTTGCTC TTTCTGCCAG CCCTCAACTG AAGGATGCCC ATTCAGAAAA GGAAGATGCT
    CCGAAGCCAC TTGAGAACGT TGTGGTGTGT GTTAGTAAGA AACTCAGTAA GAAGCAGAGT
    GAACTAAATG GGATTGCGGC CTCTCTAGGT GCAGACTACA GGTGGAGTTT TGATGAGACA
    GTGACTCATT TCATCTATCA AGGACGACCA AATGACACTA ATCGGGAGTA TAAATCTGTA
    AAAGAAAGAG GAGTACACAT TGTTTCAGAG CACTGGCTTT TAGAGTGTGC CCAGGAGTAT
    AAACATCTTC CCGAATCTCT TTATCCACAT ACTTACAATC CCAAAATGAG CTTGGACATC
    AGTGCAGTGC AAGATGGCCG ACTCTGTAAT AACCGACTAC TTTCAGCTGG CTCTGCAATG
    AGGGATGATG AGCCAGATCA TATGCCTCTA GAAGAAAATG ATATAGACAA TATGACCACC
    AGTAATAAAG AACTAACAAC ATCAAATGGA AATGGGAGGA ATGACTCTAA AGGAGCTCTG
    ACACAGACTT TGGAGATGAG AGAGAACTTT CAAAAGCAGC TTCAGGAGAT AATGTCTGCA
    ACTTCAATAG TGAAACCCCA AGGGCAGAGG ACTTCCCTCT CAAGAAGTGG TTGTAACAGT
    ACCTCTTCAA CTCCTGACAG CACCCGCTCT GCCCGCAGTG GACGAAGTAG AGTCCTAGAG
    GCACTGAGGC AGTCTCGTCA AACAGTACCT GATGTCAACA CAGAGCCCTC CCAAAATGAA
    CAGATCATTT GGGATGACCC TACAGCCAGG GAGGAGAGAG CAAGACTTGC CAGCAATTTG
    CAGTGGCCTA GTTGTCCCAC ACAATACTCT GAGCTTCAGG TTGATGTTAA AAAACTGGAG
    GATTCTCCTT TCCAGGAGCC TTTACATGAT TCAGAAATTG CTGAACAGGC TGTCTGTGAT
    CCTGGGAACG TATGTGTGAC CGGAGCCCCA AAACACCCGA TCTCTGAAGA ACTGGAAGCT
    CCAGTGAAAG ACAGCCACCT GATCCCTACG CCTCAAGCCC CCAGTATTGC CTTCCCCCTA
    GCCAACCCAC CTGTGGCTCC ACACCCTAGA GAAAAGATTA TAACAATAGA GGAGACTCAT
    GAAGACTTAA AAAAACAGTA CATATTTCAG TTGTCATCTC TGAATCCTCA AGAACGTATT
    GATTATTGTC ATCTGATTGA AAAACTAGGT GGATCAGTGA TTGAGAAGCA GTGCTTTGAT
    CCCAGCTGTA CACACATTGT TGTGGGGCAT CCACTTCGCA ACGAGAAGTA TTTAGCCTCC
    GTGGCAGCGG GAAAGTGGGT ACTCCATCGC TCCTACCTCG AAGCTTGCAG GACTGCTGGG
    CGCTTCATGT CGGAAGAAGA CTATGAGTGG GGAAGTAGTT CCATACTTGA TGTTTTGACT
    GGAATCAATG TACAGCAACG AAAACTAGCA CTTGCAGCAA TGAGATGGAG GAAAAAAATC
    CAGCAAAGAC AAGAATCAGG CATTACTGAG GGGGCATTTA GCGGGTGGAA AGTTATTTTA
    CATGTTGATC AGTCCCGAGA ATCAGGATTC AAACGCCTAC TTCAGTCAGG AGGAGCAAAG
    GTGCTGCCTG GTTATTCTGT ATCTTTATTT AAAGAAGCTA CACATCTCTT TTCTGACTTT
    AATAAACAGA AACCAGATGA CTCAGGAGTT AATATAGCAG AAGCTGCTGC CCAGAATGTA
    TACTGCTTGA AAACAGAATA CATTGCTGAT TTTCTTATGC AGGTCTCAGG AGTAGGTCAA
    AGCAGAAGAC ATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329012.1
    CDS171..4628
    Translation

    Target ORF information:

    RefSeq Version XM_015473526.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473526.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCCAAAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCT 
    GAATGTTTTT TTGAAGCTCT TGAGTCAATA AAAGAGTTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAAATA AAGGAGAATG ATAGATCACT GTATATCTGT
    GACCCCTTTA GTGGTGATGC CTTTGATCAT CTCAAAAAGC TTGGTTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC TGAACATCCA
    GTTTATAACA TGGTTATGTC TGATGTAACC GTGTCCTGTA CAAGCCTGGA AAAAGACAGA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GCGTATACAG AGACCTTAAC
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAG
    GAGAAGAAAA TAGCTAGATA TACTGATATA AACATGGAAG ACTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTACCTTAG ACAGGAAATC AGTTCAGCAG
    CTAACAGTAA AGCATGGAGG ACAGTACATG GGACAGTTGA AAATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CGCAGTGGTT TTTTGACAGT GTTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ATCAGAAGCA AAGAGTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGCT GCATAAATGA ATCAATATGT AATTCAGTTA ATAGCAAACT GGAACCTACA
    ATTGAAAATC TGGAAAATTT GGATATCAGT GCATTTCAGG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TCTATCTTTG TGGTTTTAAT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAT CAGCTCAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATG ATGATGATGA ATTGAAGCAG TTTTGGGATA AATCAGCCCA CAGGCCGCAT
    GTAGTGGGAG CAAAATGGTT GCTAGAGTGT TTTAGTAAAG GCTACATGCT TCCTGAAGAG
    CCATACATCC ACGTTAACTA CCAGCCAGTG GAAATTCCAG TTTCAGATCC ACCTGAAAGT
    AAAACAGCCC TTTTGAAAAG GAAGAACAAC GGCTTCTCTA AGAGTGACTT TGTTCCTGAG
    GAGAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAGT ATCAGACTAA TAACCCCACA
    GTAGTTGAAG TTGCCAAGTC TGAAGTTGGG CTCTGTAGTG ACTCTACTCA TGTGGAGCCC
    CGTAATGATT CTACTCATAT TTCTCTGCAA GAACACCAGT CTTCCATCAG TCATTGCCTC
    CCTGATGTTT CTACAATTAC CGAAGAAGGT TTATTTAGCC AAAAGAGTTT CCTAGTTTTG
    GGGTTTAGTA ATGAAAATGA ATCTAATATT GCAATCATCA TAAGAGAAAA TGCTGGAAAA
    ATTGTGTCCC TTCAGAGCAG AGTTGTTGCT GATTATGCTG TGGTTCCTCT GCTGGGATGT
    GAAGTACAGG CAACTGTGGG AGAAGTTGTA ACAAACACAT GGCTGGTAAT GTGTATAGAC
    TACCAGACTT TAATTGATCC AAAGTCAAAT CCTCTCTTCA CACCAGTTCC AGTTATGGCA
    GGAATGACTC CTTTAGAGGG TTGTGTTCTT TCATTCAGCC AATGTGTAGG AGCTGAAAAA
    GATTCATTGA CATTCTTAGC AAATCACCTT GGAGCCAGTG TTCAAGAATA CTTCGTTCGC
    AAGTCCAATG CAAAGAAAGG CATGTTTGCT AGTACACATC TTGTTTTGAA AGAGCCAGGT
    GGTTCTAAAT ATGAAGCTGC CAAGAAGTGG AATTTACCAG CAGTCACCAT AGCTTGGCTG
    TTAGAGTCTG CTAGATTGGG AAAGAGAGCA AATGAAAACC ATTTCCTGAT TGAAAATTCA
    TCTAAAGAAG AACAAAGTTT GGAAACTGAA ACAACAAATG AGGTGAATCT AAATCCAGAT
    ACTCCAGAGC ATCCTGTAAC ACATCTGGAA ACTCAGAGAA AAACAGCCGT TACACCTTTA
    GATATGAACC GCTTTCAGAG TAAAGCTTTC CGCACGGTGA TGTCACAGCA CTCCGGACAG
    GCTTCAGTGT CCCCTTCCCC AGGACAGTCA CTTCAGAAGG AGCCCTCATT ACACCTGGAT
    ACGCCGTCAA AGTTTCTGTC CAAGGACAAA CTGTTCAAGC CTTCCTTTGA TGTGAAGGAT
    GCTCTCGCAG CCTTGGAAAC TCCAGGAGGT CCCAGCCAGC AGAAAAGGAA ACTGAGTACA
    CCACTCTCAG AAGTCATCGT CAAAAACCTG AAACTTGCTT TGGCAAATAG CTCTCGAAAT
    GCTGTTGCTC TTTCTGCCAG CCCTCAACTG AAGGATGCCC ATTCAGAAAA GGAAGATGCT
    CCGAAGCCAC TTGAGAACGT TGTGGTGTGT GTTAGTAAGA AACTCAGTAA GAAGCAGAGT
    GAACTAAATG GGATTGCGGC CTCTCTAGGT GCAGACTACA GGTGGAGTTT TGATGAGACA
    GTGACTCATT TCATCTATCA AGGACGACCA AATGACACTA ATCGGGAGTA TAAATCTGTA
    AAAGAAAGAG GAGTACACAT TGTTTCAGAG CACTGGCTTT TAGAGTGTGC CCAGGAGTAT
    AAACATCTTC CCGAATCTCT TTATCCACAT ACTTACAATC CCAAAATGAG CTTGGACATC
    AGTGCAGTGC AAGATGGCCG ACTCTGTAAT AACCGACTAC TTTCAGCTGG CTCTGCAATG
    AGGGATGATG AGCCAGATCA TATGCCTCTA GAAGAAAATG ATATAGACAA TATGACCACC
    AGTAATAAAG AACTAACAAC ATCAAATGGA AATGGGAGGA ATGACTCTAA AGGAGCTCTG
    ACACAGACTT TGGAGATGAG AGAGAACTTT CAAAAGCAGC TTCAGGAGAT AATGTCTGCA
    ACTTCAATAG TGAAACCCCA AGGGCAGAGG ACTTCCCTCT CAAGAAGTGG TTGTAACAGT
    ACCTCTTCAA CTCCTGACAG CACCCGCTCT GCCCGCAGTG GACGAAGTAG AGTCCTAGAG
    GCACTGAGGC AGTCTCGTCA AACAGTACCT GATGTCAACA CAGAGCCCTC CCAAAATGAA
    CAGATCATTT GGGATGACCC TACAGCCAGG GAGGAGAGAG CAAGACTTGC CAGCAATTTG
    CAGTGGCCTA GTTGTCCCAC ACAATACTCT GAGCTTCAGG TTGATGTTAA AAAACTGGAG
    GATTCTCCTT TCCAGGAGCC TTTACATGAT TCAGAAATTG CTGAACAGGC TGTCTGTGAT
    CCTGGGAACG TATGTGTGAC CGGAGCCCCA AAACACCCGA TCTCTGAAGA ACTGGAAGCT
    CCAGTGAAAG ACAGCCACCT GATCCCTACG CCTCAAGCCC CCAGTATTGC CTTCCCCCTA
    GCCAACCCAC CTGTGGCTCC ACACCCTAGA GAAAAGATTA TAACAATAGA GGAGACTCAT
    GAAGACTTAA AAAAACAGTA CATATTTCAG TTGTCATCTC TGAATCCTCA AGAACGTATT
    GATTATTGTC ATCTGATTGA AAAACTAGGT GGATCAGTGA TTGAGAAGCA GTGCTTTGAT
    CCCAGCTGTA CACACATTGT TGTGGGGCAT CCACTTCGCA ACGAGAAGTA TTTAGCCTCC
    GTGGCAGCGG GAAAGTGGGT ACTCCATCGC TCCTACCTCG AAGCTTGCAG GACTGCTGGG
    CGCTTCATGT CGGAAGAAGA CTATGAGTGG GGAAGTAGTT CCATACTTGA TGTTTTGACT
    GGAATCAATG TACAGCAACG AAAACTAGCA CTTGCAGCAA TGAGATGGAG GAAAAAAATC
    CAGCAAAGAC AAGAATCAGG CATTACTGAG GGGGCATTTA GCGGGTGGAA AGTTATTTTA
    CATGTTGATC AGTCCCGAGA ATCAGGATTC AAACGCCTAC TTCAGTCAGG AGGAGCAAAG
    GTGCTGCCTG GTTATTCTGT ATCTTTATTT AAAGAAGCTA CACATCTCTT TTCTGACTTT
    AATAAACAGA AACCAGATGA CTCAGGAGTT AATATAGCAG AAGCTGCTGC CCAGAATGTA
    TACTGCTTGA AAACAGAATA CATTGCTGAT TTTCTTATGC AGGTCTCAGG AGTAGGTCAA
    AGCAGAAGAC ATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16054
    Clone ID Related Accession (Same CDS sequence) NM_001206766.2 , NM_001206766.1
    Accession Version NM_001206766.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4566bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-07-13
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-binding protein 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000001.1. On May 30, 2018 this sequence version replaced NM_001206766.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571259.34693.1, SRR5571259.35254.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145431 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCCAAAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCT 
    GAATGTTTTT TTGAAGCTCT TGAGTCAATA AAAGAGTTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAAATA AAGGAGAATG ATAGATCACT GTATATCTGT
    GACCCCTTTA GTGGTGATGC CTTTGATCAT CTCAAAAAGC TTGGTTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC TGAACATCCA
    GTTTATAACA TGGTTATGTC TGATGTAACC GTGTCCTGTA CAAGCCTGGA AAAAGACAGA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GCGTATACAG AGACCTTAAC
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAG
    GAGAAGAAAA TAGCTAGATA TACTGATATA AACATGGAAG ACTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTACCTTAG ACAGGAAATC AGTTCAGCAG
    CTAACAGTAA AGCATGGAGG ACAGTACATG GGACAGTTGA AAATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CGCAGTGGTT TTTTGACAGT GTTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ATCAGAAGCA AAGAGTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGCT GCATAAATGA ATCAATATGT AATTCAGTTA ATAGCAAACT GGAACCTACA
    ATTGAAAATC TGGAAAATTT GGATATCAGT GCATTTCAGG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TCTATCTTTG TGGTTTTAAT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAT CAGCTCAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATG ATGATGATGA ATTGAAGCAG TTTTGGGATA AATCAGCCCA CAGGCCGCAT
    GTAGTGGGAG CAAAATGGTT GCTAGAGTGT TTTAGTAAAG GCTACATGCT TCCTGAAGAG
    CCATACATCC ACGTTAACTA CCAGCCAGTG GAAATTCCAG TTTCAGATCC ACCTGAAAGT
    AAAACAGCCC TTTTGAAAAG GAAGAACAAC GGCTTCTCTA AGAGTGACTT TGTTCCTGAG
    GAGAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAGT ATCAGACTAA TAACCCCACA
    GTAGTTGAAG TTGCCAAGTC TGAAGTTGGG CTCTGTAGTG ACTCTACTCA TGTGGAGCCC
    CGTAATGATT CTACTCATAT TTCTCTGCAA GAACACCAGT CTTCCATCAG TCATTGCCTC
    CCTGATGTTT CTACAATTAC CGAAGAAGGT TTATTTAGCC AAAAGAGTTT CCTAGTTTTG
    GGGTTTAGTA ATGAAAATGA ATCTAATATT GCAATCATCA TAAGAGAAAA TGCTGGAAAA
    ATTGTGTCCC TTCAGAGCAG AGTTGTTGCT GATTATGCTG TGGTTCCTCT GCTGGGATGT
    GAAGTACAGG CAACTGTGGG AGAAGTTGTA ACAAACACAT GGCTGGTAAT GTGTATAGAC
    TACCAGACTT TAATTGATCC AAAGTCAAAT CCTCTCTTCA CACCAGTTCC AGTTATGGCA
    GGAATGACTC CTTTAGAGGG TTGTGTTCTT TCATTCAGCC AATGTGTAGG AGCTGAAAAA
    GATTCATTGA CATTCTTAGC AAATCACCTT GGAGCCAGTG TTCAAGAATA CTTCGTTCGC
    AAGTCCAATG CAAAGAAAGG CATGTTTGCT AGTACACATC TTGTTTTGAA AGAGCCAGGT
    GGTTCTAAAT ATGAAGCTGC CAAGAAGTGG AATTTACCAG CAGTCACCAT AGCTTGGCTG
    TTAGAGTCTG CTAGATTGGG AAAGAGAGCA AATGAAAACC ATTTCCTGAT TGAAAATTCA
    TCTAAAGAAG AACAAAGTTT GGAAACTGAA ACAACAAATG AGGTGAATCT AAATCCAGAT
    ACTCCAGAGC ATCCTGTAAC ACATCTGGAA ACTCAGAGAA AAACAGCCGT TACACCTTTA
    GATATGAACC GCTTTCAGAG TAAAGCTTTC CGCACGGTGA TGTCACAGCA CTCCGGACAG
    GCTTCAGTGT CCCCTTCCCC AGGACAGTCA CTTCAGAAGG AGCCCTCATT ACACCTGGAT
    ACGCCGTCAA AGTTTCTGTC CAAGGACAAA CTGTTCAAGC CTTCCTTTGA TGTGAAGGAT
    GCTCTCGCAG CCTTGGAAAC TCCAGGAGGT CCCAGCCAGC AGAAAAGGAA ACTGAGTACA
    CCACTCTCAG AAGTCATCGT CAAAAACCTG AAACTTGCTT TGGCAAATAG CTCTCGAAAT
    GCTGTTGCTC TTTCTGCCAG CCCTCAACTG AAGGATGCCC ATTCAGAAAA GGAAGATGCT
    CCGAAGCCAC TTGAGAACGT TGTGGTGTGT GTTAGTAAGA AACTCAGTAA GAAGCAGAGT
    GAACTAAATG GGATTGCGGC CTCTCTAGGT GCAGACTACA GGTGGAGTTT TGATGAGACA
    GTGACTCATT TCATCTATCA AGGACGACCA AATGACACTA ATCGGGAGTA TAAATCTGTA
    AAAGAAAGAG GAGTACACAT TGTTTCAGAG CACTGGCTTT TAGAGTGTGC CCAGGAGTAT
    AAACATCTTC CCGAATCTCT TTATCCACAT ACTTACAATC CCAAAATGAG CTTGGACATC
    AGTGCAGTGC AAGATGGCCG ACTCTGTAAT AACCGACTAC TTTCAGCTGG CTCTGCAATG
    AGGGATGATG AGCCAGATCA TATGCCTCTA GAAGAAAATG ATATAGACAA TATGACCACC
    AGTAATAAAG AACTAACAAC ATCAAATGGA AATGGGAGGA ATGACTCTAA AGGAGCTCTG
    ACACAGACTT TGGAGATGAG AGAGAACTTT CAAAAGCAGC TTCAGGAGAT AATGTCTGCA
    ACTTCAATAG TGAAACCCCA AGGGCAGAGG ACTTCCCTCT CAAGAAGTGG TTGTAACAGT
    ACCTCTTCAA CTCCTGACAG CACCCGCTCT GCCCGCAGTG GACGAAGTAG AGTCCTAGAG
    GCACTGAGGC AGTCTCGTCA AACAGTACCT GATGTCAACA CAGAGCCCTC CCAAAATGAA
    CAGATCATTT GGGATGACCC TACAGCCAGG GAGGAGAGAG CAAGACTTGC CAGCAATTTG
    CAGTGGCCTA GTTGTCCCAC ACAATACTCT GAGCTTCAGG TTGATGTTAA AAAACTGGAG
    GATTCTCCTT TCCAGGAGCC TTTACATGAT TCAGAAATTG CTGAACAGGC TGTCTGTGAT
    CCTGGGAACG TATGTGTGAC CGGAGCCCCA AAACACCCGA TCTCTGAAGA ACTGGAAGCT
    CCAGTGAAAG ACAGCCACCT GATCCCTACG CCTCAAGCCC CCAGTATTGC CTTCCCCCTA
    GCCAACCCAC CTGTGGCTCC ACACCCTAGA GAAAAGATTA TAACAATAGA GGAGACTCAT
    GAAGACTTAA AAAAACAGTA CATATTTCAG TTGTCATCTC TGAATCCTCA AGAACGTATT
    GATTATTGTC ATCTGATTGA AAAACTAGGT GGATCAGTGA TTGAGAAGCA GTGCTTTGAT
    CCCAGCTGTA CACACATTGT TGTGGGGCAT CCACTTCGCA ACGAGAAGTA TTTAGCCTCC
    GTGGCAGCGG GAAAGTGGGT ACTCCATCGC TCCTACCTCG AAGCTTGCAG GACTGCTGGG
    CGCTTCATGT CGGAAGAAGA CTATGAGTGG GGAAGTAGTT CCATACTTGA TGTTTTGACT
    GGAATCAATG TACAGCAACG AAAACTAGCA CTTGCAGCAA TGAGATGGAG GAAAAAAATC
    CAGCAAAGAC AAGAATCAGG CATTACTGAG GGGGCATTTA GCGGGTGGAA AGTTATTTTA
    CATGTTGATC AGTCCCGAGA ATCAGGATTC AAACGCCTAC TTCAGTCAGG AGGAGCAAAG
    GTGCTGCCTG GTTATTCTGT ATCTTTATTT AAAGAAGCTA CACATCTCTT TTCTGACTTT
    AATAAACAGA AACCAGATGA CTCAGGAGTT AATATAGCAG AAGCTGCTGC CCAGAATGTA
    TACTGCTTGA AAACAGAATA CATTGCTGAT TTTCTTATGC AGGAATCCCC TCCTCAAGTA
    GAAAATTACT GTCTACCAGA AGCTGCTTCA TTTCTTCAGA ATAAAAAGGA ACCTGGGCCT
    GGATTCTCAC AAAAGAGGAA AGCTCCTGCA GAAAAAAATA AAATCAAACG ACCCAGAGTA
    CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193695.1
    CDS134..4699
    Translation

    Target ORF information:

    RefSeq Version NM_001206766.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II binding protein 1 (TOPBP1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206766.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCCAAAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCT 
    GAATGTTTTT TTGAAGCTCT TGAGTCAATA AAAGAGTTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAAATA AAGGAGAATG ATAGATCACT GTATATCTGT
    GACCCCTTTA GTGGTGATGC CTTTGATCAT CTCAAAAAGC TTGGTTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC TGAACATCCA
    GTTTATAACA TGGTTATGTC TGATGTAACC GTGTCCTGTA CAAGCCTGGA AAAAGACAGA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GCGTATACAG AGACCTTAAC
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAG
    GAGAAGAAAA TAGCTAGATA TACTGATATA AACATGGAAG ACTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTACCTTAG ACAGGAAATC AGTTCAGCAG
    CTAACAGTAA AGCATGGAGG ACAGTACATG GGACAGTTGA AAATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CGCAGTGGTT TTTTGACAGT GTTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ATCAGAAGCA AAGAGTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGCT GCATAAATGA ATCAATATGT AATTCAGTTA ATAGCAAACT GGAACCTACA
    ATTGAAAATC TGGAAAATTT GGATATCAGT GCATTTCAGG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TCTATCTTTG TGGTTTTAAT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAT CAGCTCAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATG ATGATGATGA ATTGAAGCAG TTTTGGGATA AATCAGCCCA CAGGCCGCAT
    GTAGTGGGAG CAAAATGGTT GCTAGAGTGT TTTAGTAAAG GCTACATGCT TCCTGAAGAG
    CCATACATCC ACGTTAACTA CCAGCCAGTG GAAATTCCAG TTTCAGATCC ACCTGAAAGT
    AAAACAGCCC TTTTGAAAAG GAAGAACAAC GGCTTCTCTA AGAGTGACTT TGTTCCTGAG
    GAGAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAGT ATCAGACTAA TAACCCCACA
    GTAGTTGAAG TTGCCAAGTC TGAAGTTGGG CTCTGTAGTG ACTCTACTCA TGTGGAGCCC
    CGTAATGATT CTACTCATAT TTCTCTGCAA GAACACCAGT CTTCCATCAG TCATTGCCTC
    CCTGATGTTT CTACAATTAC CGAAGAAGGT TTATTTAGCC AAAAGAGTTT CCTAGTTTTG
    GGGTTTAGTA ATGAAAATGA ATCTAATATT GCAATCATCA TAAGAGAAAA TGCTGGAAAA
    ATTGTGTCCC TTCAGAGCAG AGTTGTTGCT GATTATGCTG TGGTTCCTCT GCTGGGATGT
    GAAGTACAGG CAACTGTGGG AGAAGTTGTA ACAAACACAT GGCTGGTAAT GTGTATAGAC
    TACCAGACTT TAATTGATCC AAAGTCAAAT CCTCTCTTCA CACCAGTTCC AGTTATGGCA
    GGAATGACTC CTTTAGAGGG TTGTGTTCTT TCATTCAGCC AATGTGTAGG AGCTGAAAAA
    GATTCATTGA CATTCTTAGC AAATCACCTT GGAGCCAGTG TTCAAGAATA CTTCGTTCGC
    AAGTCCAATG CAAAGAAAGG CATGTTTGCT AGTACACATC TTGTTTTGAA AGAGCCAGGT
    GGTTCTAAAT ATGAAGCTGC CAAGAAGTGG AATTTACCAG CAGTCACCAT AGCTTGGCTG
    TTAGAGTCTG CTAGATTGGG AAAGAGAGCA AATGAAAACC ATTTCCTGAT TGAAAATTCA
    TCTAAAGAAG AACAAAGTTT GGAAACTGAA ACAACAAATG AGGTGAATCT AAATCCAGAT
    ACTCCAGAGC ATCCTGTAAC ACATCTGGAA ACTCAGAGAA AAACAGCCGT TACACCTTTA
    GATATGAACC GCTTTCAGAG TAAAGCTTTC CGCACGGTGA TGTCACAGCA CTCCGGACAG
    GCTTCAGTGT CCCCTTCCCC AGGACAGTCA CTTCAGAAGG AGCCCTCATT ACACCTGGAT
    ACGCCGTCAA AGTTTCTGTC CAAGGACAAA CTGTTCAAGC CTTCCTTTGA TGTGAAGGAT
    GCTCTCGCAG CCTTGGAAAC TCCAGGAGGT CCCAGCCAGC AGAAAAGGAA ACTGAGTACA
    CCACTCTCAG AAGTCATCGT CAAAAACCTG AAACTTGCTT TGGCAAATAG CTCTCGAAAT
    GCTGTTGCTC TTTCTGCCAG CCCTCAACTG AAGGATGCCC ATTCAGAAAA GGAAGATGCT
    CCGAAGCCAC TTGAGAACGT TGTGGTGTGT GTTAGTAAGA AACTCAGTAA GAAGCAGAGT
    GAACTAAATG GGATTGCGGC CTCTCTAGGT GCAGACTACA GGTGGAGTTT TGATGAGACA
    GTGACTCATT TCATCTATCA AGGACGACCA AATGACACTA ATCGGGAGTA TAAATCTGTA
    AAAGAAAGAG GAGTACACAT TGTTTCAGAG CACTGGCTTT TAGAGTGTGC CCAGGAGTAT
    AAACATCTTC CCGAATCTCT TTATCCACAT ACTTACAATC CCAAAATGAG CTTGGACATC
    AGTGCAGTGC AAGATGGCCG ACTCTGTAAT AACCGACTAC TTTCAGCTGG CTCTGCAATG
    AGGGATGATG AGCCAGATCA TATGCCTCTA GAAGAAAATG ATATAGACAA TATGACCACC
    AGTAATAAAG AACTAACAAC ATCAAATGGA AATGGGAGGA ATGACTCTAA AGGAGCTCTG
    ACACAGACTT TGGAGATGAG AGAGAACTTT CAAAAGCAGC TTCAGGAGAT AATGTCTGCA
    ACTTCAATAG TGAAACCCCA AGGGCAGAGG ACTTCCCTCT CAAGAAGTGG TTGTAACAGT
    ACCTCTTCAA CTCCTGACAG CACCCGCTCT GCCCGCAGTG GACGAAGTAG AGTCCTAGAG
    GCACTGAGGC AGTCTCGTCA AACAGTACCT GATGTCAACA CAGAGCCCTC CCAAAATGAA
    CAGATCATTT GGGATGACCC TACAGCCAGG GAGGAGAGAG CAAGACTTGC CAGCAATTTG
    CAGTGGCCTA GTTGTCCCAC ACAATACTCT GAGCTTCAGG TTGATGTTAA AAAACTGGAG
    GATTCTCCTT TCCAGGAGCC TTTACATGAT TCAGAAATTG CTGAACAGGC TGTCTGTGAT
    CCTGGGAACG TATGTGTGAC CGGAGCCCCA AAACACCCGA TCTCTGAAGA ACTGGAAGCT
    CCAGTGAAAG ACAGCCACCT GATCCCTACG CCTCAAGCCC CCAGTATTGC CTTCCCCCTA
    GCCAACCCAC CTGTGGCTCC ACACCCTAGA GAAAAGATTA TAACAATAGA GGAGACTCAT
    GAAGACTTAA AAAAACAGTA CATATTTCAG TTGTCATCTC TGAATCCTCA AGAACGTATT
    GATTATTGTC ATCTGATTGA AAAACTAGGT GGATCAGTGA TTGAGAAGCA GTGCTTTGAT
    CCCAGCTGTA CACACATTGT TGTGGGGCAT CCACTTCGCA ACGAGAAGTA TTTAGCCTCC
    GTGGCAGCGG GAAAGTGGGT ACTCCATCGC TCCTACCTCG AAGCTTGCAG GACTGCTGGG
    CGCTTCATGT CGGAAGAAGA CTATGAGTGG GGAAGTAGTT CCATACTTGA TGTTTTGACT
    GGAATCAATG TACAGCAACG AAAACTAGCA CTTGCAGCAA TGAGATGGAG GAAAAAAATC
    CAGCAAAGAC AAGAATCAGG CATTACTGAG GGGGCATTTA GCGGGTGGAA AGTTATTTTA
    CATGTTGATC AGTCCCGAGA ATCAGGATTC AAACGCCTAC TTCAGTCAGG AGGAGCAAAG
    GTGCTGCCTG GTTATTCTGT ATCTTTATTT AAAGAAGCTA CACATCTCTT TTCTGACTTT
    AATAAACAGA AACCAGATGA CTCAGGAGTT AATATAGCAG AAGCTGCTGC CCAGAATGTA
    TACTGCTTGA AAACAGAATA CATTGCTGAT TTTCTTATGC AGGAATCCCC TCCTCAAGTA
    GAAAATTACT GTCTACCAGA AGCTGCTTCA TTTCTTCAGA ATAAAAAGGA ACCTGGGCCT
    GGATTCTCAC AAAAGAGGAA AGCTCCTGCA GAAAAAAATA AAATCAAACG ACCCAGAGTA
    CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16054
    Clone ID Related Accession (Same CDS sequence) NM_001206766.2 , NM_001206766.1
    Accession Version NM_001206766.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4566bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-binding protein 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02003073.1 and DAAA02003074.1. On or before May 6, 2011 this sequence version replaced XM_002685092.1, XM_612160.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02403640, SAMN02403648 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCCAAAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCT 
    GAATGTTTTT TTGAAGCTCT TGAGTCAATA AAAGAGTTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAAATA AAGGAGAATG ATAGATCACT GTATATCTGT
    GACCCCTTTA GTGGTGATGC CTTTGATCAT CTCAAAAAGC TTGGTTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC TGAACATCCA
    GTTTATAACA TGGTTATGTC TGATGTAACC GTGTCCTGTA CAAGCCTGGA AAAAGACAGA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GCGTATACAG AGACCTTAAC
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAG
    GAGAAGAAAA TAGCTAGATA TACTGATATA AACATGGAAG ACTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTACCTTAG ACAGGAAATC AGTTCAGCAG
    CTAACAGTAA AGCATGGAGG ACAGTACATG GGACAGTTGA AAATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CGCAGTGGTT TTTTGACAGT GTTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ATCAGAAGCA AAGAGTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGCT GCATAAATGA ATCAATATGT AATTCAGTTA ATAGCAAACT GGAACCTACA
    ATTGAAAATC TGGAAAATTT GGATATCAGT GCATTTCAGG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TCTATCTTTG TGGTTTTAAT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAT CAGCTCAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATG ATGATGATGA ATTGAAGCAG TTTTGGGATA AATCAGCCCA CAGGCCGCAT
    GTAGTGGGAG CAAAATGGTT GCTAGAGTGT TTTAGTAAAG GCTACATGCT TCCTGAAGAG
    CCATACATCC ACGTTAACTA CCAGCCAGTG GAAATTCCAG TTTCAGATCC ACCTGAAAGT
    AAAACAGCCC TTTTGAAAAG GAAGAACAAC GGCTTCTCTA AGAGTGACTT TGTTCCTGAG
    GAGAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAGT ATCAGACTAA TAACCCCACA
    GTAGTTGAAG TTGCCAAGTC TGAAGTTGGG CTCTGTAGTG ACTCTACTCA TGTGGAGCCC
    CGTAATGATT CTACTCATAT TTCTCTGCAA GAACACCAGT CTTCCATCAG TCATTGCCTC
    CCTGATGTTT CTACAATTAC CGAAGAAGGT TTATTTAGCC AAAAGAGTTT CCTAGTTTTG
    GGGTTTAGTA ATGAAAATGA ATCTAATATT GCAATCATCA TAAGAGAAAA TGCTGGAAAA
    ATTGTGTCCC TTCAGAGCAG AGTTGTTGCT GATTATGCTG TGGTTCCTCT GCTGGGATGT
    GAAGTACAGG CAACTGTGGG AGAAGTTGTA ACAAACACAT GGCTGGTAAT GTGTATAGAC
    TACCAGACTT TAATTGATCC AAAGTCAAAT CCTCTCTTCA CACCAGTTCC AGTTATGGCA
    GGAATGACTC CTTTAGAGGG TTGTGTTCTT TCATTCAGCC AATGTGTAGG AGCTGAAAAA
    GATTCATTGA CATTCTTAGC AAATCACCTT GGAGCCAGTG TTCAAGAATA CTTCGTTCGC
    AAGTCCAATG CAAAGAAAGG CATGTTTGCT AGTACACATC TTGTTTTGAA AGAGCCAGGT
    GGTTCTAAAT ATGAAGCTGC CAAGAAGTGG AATTTACCAG CAGTCACCAT AGCTTGGCTG
    TTAGAGTCTG CTAGATTGGG AAAGAGAGCA AATGAAAACC ATTTCCTGAT TGAAAATTCA
    TCTAAAGAAG AACAAAGTTT GGAAACTGAA ACAACAAATG AGGTGAATCT AAATCCAGAT
    ACTCCAGAGC ATCCTGTAAC ACATCTGGAA ACTCAGAGAA AAACAGCCGT TACACCTTTA
    GATATGAACC GCTTTCAGAG TAAAGCTTTC CGCACGGTGA TGTCACAGCA CTCCGGACAG
    GCTTCAGTGT CCCCTTCCCC AGGACAGTCA CTTCAGAAGG AGCCCTCATT ACACCTGGAT
    ACGCCGTCAA AGTTTCTGTC CAAGGACAAA CTGTTCAAGC CTTCCTTTGA TGTGAAGGAT
    GCTCTCGCAG CCTTGGAAAC TCCAGGAGGT CCCAGCCAGC AGAAAAGGAA ACTGAGTACA
    CCACTCTCAG AAGTCATCGT CAAAAACCTG AAACTTGCTT TGGCAAATAG CTCTCGAAAT
    GCTGTTGCTC TTTCTGCCAG CCCTCAACTG AAGGATGCCC ATTCAGAAAA GGAAGATGCT
    CCGAAGCCAC TTGAGAACGT TGTGGTGTGT GTTAGTAAGA AACTCAGTAA GAAGCAGAGT
    GAACTAAATG GGATTGCGGC CTCTCTAGGT GCAGACTACA GGTGGAGTTT TGATGAGACA
    GTGACTCATT TCATCTATCA AGGACGACCA AATGACACTA ATCGGGAGTA TAAATCTGTA
    AAAGAAAGAG GAGTACACAT TGTTTCAGAG CACTGGCTTT TAGAGTGTGC CCAGGAGTAT
    AAACATCTTC CCGAATCTCT TTATCCACAT ACTTACAATC CCAAAATGAG CTTGGACATC
    AGTGCAGTGC AAGATGGCCG ACTCTGTAAT AACCGACTAC TTTCAGCTGG CTCTGCAATG
    AGGGATGATG AGCCAGATCA TATGCCTCTA GAAGAAAATG ATATAGACAA TATGACCACC
    AGTAATAAAG AACTAACAAC ATCAAATGGA AATGGGAGGA ATGACTCTAA AGGAGCTCTG
    ACACAGACTT TGGAGATGAG AGAGAACTTT CAAAAGCAGC TTCAGGAGAT AATGTCTGCA
    ACTTCAATAG TGAAACCCCA AGGGCAGAGG ACTTCCCTCT CAAGAAGTGG TTGTAACAGT
    ACCTCTTCAA CTCCTGACAG CACCCGCTCT GCCCGCAGTG GACGAAGTAG AGTCCTAGAG
    GCACTGAGGC AGTCTCGTCA AACAGTACCT GATGTCAACA CAGAGCCCTC CCAAAATGAA
    CAGATCATTT GGGATGACCC TACAGCCAGG GAGGAGAGAG CAAGACTTGC CAGCAATTTG
    CAGTGGCCTA GTTGTCCCAC ACAATACTCT GAGCTTCAGG TTGATGTTAA AAAACTGGAG
    GATTCTCCTT TCCAGGAGCC TTTACATGAT TCAGAAATTG CTGAACAGGC TGTCTGTGAT
    CCTGGGAACG TATGTGTGAC CGGAGCCCCA AAACACCCGA TCTCTGAAGA ACTGGAAGCT
    CCAGTGAAAG ACAGCCACCT GATCCCTACG CCTCAAGCCC CCAGTATTGC CTTCCCCCTA
    GCCAACCCAC CTGTGGCTCC ACACCCTAGA GAAAAGATTA TAACAATAGA GGAGACTCAT
    GAAGACTTAA AAAAACAGTA CATATTTCAG TTGTCATCTC TGAATCCTCA AGAACGTATT
    GATTATTGTC ATCTGATTGA AAAACTAGGT GGATCAGTGA TTGAGAAGCA GTGCTTTGAT
    CCCAGCTGTA CACACATTGT TGTGGGGCAT CCACTTCGCA ACGAGAAGTA TTTAGCCTCC
    GTGGCAGCGG GAAAGTGGGT ACTCCATCGC TCCTACCTCG AAGCTTGCAG GACTGCTGGG
    CGCTTCATGT CGGAAGAAGA CTATGAGTGG GGAAGTAGTT CCATACTTGA TGTTTTGACT
    GGAATCAATG TACAGCAACG AAAACTAGCA CTTGCAGCAA TGAGATGGAG GAAAAAAATC
    CAGCAAAGAC AAGAATCAGG CATTACTGAG GGGGCATTTA GCGGGTGGAA AGTTATTTTA
    CATGTTGATC AGTCCCGAGA ATCAGGATTC AAACGCCTAC TTCAGTCAGG AGGAGCAAAG
    GTGCTGCCTG GTTATTCTGT ATCTTTATTT AAAGAAGCTA CACATCTCTT TTCTGACTTT
    AATAAACAGA AACCAGATGA CTCAGGAGTT AATATAGCAG AAGCTGCTGC CCAGAATGTA
    TACTGCTTGA AAACAGAATA CATTGCTGAT TTTCTTATGC AGGAATCCCC TCCTCAAGTA
    GAAAATTACT GTCTACCAGA AGCTGCTTCA TTTCTTCAGA ATAAAAAGGA ACCTGGGCCT
    GGATTCTCAC AAAAGAGGAA AGCTCCTGCA GAAAAAAATA AAATCAAACG ACCCAGAGTA
    CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193695.1
    CDS134..4699
    Translation

    Target ORF information:

    RefSeq Version NM_001206766.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II binding protein 1 (TOPBP1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206766.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCCAAAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCT 
    GAATGTTTTT TTGAAGCTCT TGAGTCAATA AAAGAGTTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAAATA AAGGAGAATG ATAGATCACT GTATATCTGT
    GACCCCTTTA GTGGTGATGC CTTTGATCAT CTCAAAAAGC TTGGTTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC TGAACATCCA
    GTTTATAACA TGGTTATGTC TGATGTAACC GTGTCCTGTA CAAGCCTGGA AAAAGACAGA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GCGTATACAG AGACCTTAAC
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAG
    GAGAAGAAAA TAGCTAGATA TACTGATATA AACATGGAAG ACTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTACCTTAG ACAGGAAATC AGTTCAGCAG
    CTAACAGTAA AGCATGGAGG ACAGTACATG GGACAGTTGA AAATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CGCAGTGGTT TTTTGACAGT GTTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ATCAGAAGCA AAGAGTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCCATAT TTCCAACATC
    AATGCAAGCT GCATAAATGA ATCAATATGT AATTCAGTTA ATAGCAAACT GGAACCTACA
    ATTGAAAATC TGGAAAATTT GGATATCAGT GCATTTCAGG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TCTATCTTTG TGGTTTTAAT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAT CAGCTCAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATG ATGATGATGA ATTGAAGCAG TTTTGGGATA AATCAGCCCA CAGGCCGCAT
    GTAGTGGGAG CAAAATGGTT GCTAGAGTGT TTTAGTAAAG GCTACATGCT TCCTGAAGAG
    CCATACATCC ACGTTAACTA CCAGCCAGTG GAAATTCCAG TTTCAGATCC ACCTGAAAGT
    AAAACAGCCC TTTTGAAAAG GAAGAACAAC GGCTTCTCTA AGAGTGACTT TGTTCCTGAG
    GAGAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAGT ATCAGACTAA TAACCCCACA
    GTAGTTGAAG TTGCCAAGTC TGAAGTTGGG CTCTGTAGTG ACTCTACTCA TGTGGAGCCC
    CGTAATGATT CTACTCATAT TTCTCTGCAA GAACACCAGT CTTCCATCAG TCATTGCCTC
    CCTGATGTTT CTACAATTAC CGAAGAAGGT TTATTTAGCC AAAAGAGTTT CCTAGTTTTG
    GGGTTTAGTA ATGAAAATGA ATCTAATATT GCAATCATCA TAAGAGAAAA TGCTGGAAAA
    ATTGTGTCCC TTCAGAGCAG AGTTGTTGCT GATTATGCTG TGGTTCCTCT GCTGGGATGT
    GAAGTACAGG CAACTGTGGG AGAAGTTGTA ACAAACACAT GGCTGGTAAT GTGTATAGAC
    TACCAGACTT TAATTGATCC AAAGTCAAAT CCTCTCTTCA CACCAGTTCC AGTTATGGCA
    GGAATGACTC CTTTAGAGGG TTGTGTTCTT TCATTCAGCC AATGTGTAGG AGCTGAAAAA
    GATTCATTGA CATTCTTAGC AAATCACCTT GGAGCCAGTG TTCAAGAATA CTTCGTTCGC
    AAGTCCAATG CAAAGAAAGG CATGTTTGCT AGTACACATC TTGTTTTGAA AGAGCCAGGT
    GGTTCTAAAT ATGAAGCTGC CAAGAAGTGG AATTTACCAG CAGTCACCAT AGCTTGGCTG
    TTAGAGTCTG CTAGATTGGG AAAGAGAGCA AATGAAAACC ATTTCCTGAT TGAAAATTCA
    TCTAAAGAAG AACAAAGTTT GGAAACTGAA ACAACAAATG AGGTGAATCT AAATCCAGAT
    ACTCCAGAGC ATCCTGTAAC ACATCTGGAA ACTCAGAGAA AAACAGCCGT TACACCTTTA
    GATATGAACC GCTTTCAGAG TAAAGCTTTC CGCACGGTGA TGTCACAGCA CTCCGGACAG
    GCTTCAGTGT CCCCTTCCCC AGGACAGTCA CTTCAGAAGG AGCCCTCATT ACACCTGGAT
    ACGCCGTCAA AGTTTCTGTC CAAGGACAAA CTGTTCAAGC CTTCCTTTGA TGTGAAGGAT
    GCTCTCGCAG CCTTGGAAAC TCCAGGAGGT CCCAGCCAGC AGAAAAGGAA ACTGAGTACA
    CCACTCTCAG AAGTCATCGT CAAAAACCTG AAACTTGCTT TGGCAAATAG CTCTCGAAAT
    GCTGTTGCTC TTTCTGCCAG CCCTCAACTG AAGGATGCCC ATTCAGAAAA GGAAGATGCT
    CCGAAGCCAC TTGAGAACGT TGTGGTGTGT GTTAGTAAGA AACTCAGTAA GAAGCAGAGT
    GAACTAAATG GGATTGCGGC CTCTCTAGGT GCAGACTACA GGTGGAGTTT TGATGAGACA
    GTGACTCATT TCATCTATCA AGGACGACCA AATGACACTA ATCGGGAGTA TAAATCTGTA
    AAAGAAAGAG GAGTACACAT TGTTTCAGAG CACTGGCTTT TAGAGTGTGC CCAGGAGTAT
    AAACATCTTC CCGAATCTCT TTATCCACAT ACTTACAATC CCAAAATGAG CTTGGACATC
    AGTGCAGTGC AAGATGGCCG ACTCTGTAAT AACCGACTAC TTTCAGCTGG CTCTGCAATG
    AGGGATGATG AGCCAGATCA TATGCCTCTA GAAGAAAATG ATATAGACAA TATGACCACC
    AGTAATAAAG AACTAACAAC ATCAAATGGA AATGGGAGGA ATGACTCTAA AGGAGCTCTG
    ACACAGACTT TGGAGATGAG AGAGAACTTT CAAAAGCAGC TTCAGGAGAT AATGTCTGCA
    ACTTCAATAG TGAAACCCCA AGGGCAGAGG ACTTCCCTCT CAAGAAGTGG TTGTAACAGT
    ACCTCTTCAA CTCCTGACAG CACCCGCTCT GCCCGCAGTG GACGAAGTAG AGTCCTAGAG
    GCACTGAGGC AGTCTCGTCA AACAGTACCT GATGTCAACA CAGAGCCCTC CCAAAATGAA
    CAGATCATTT GGGATGACCC TACAGCCAGG GAGGAGAGAG CAAGACTTGC CAGCAATTTG
    CAGTGGCCTA GTTGTCCCAC ACAATACTCT GAGCTTCAGG TTGATGTTAA AAAACTGGAG
    GATTCTCCTT TCCAGGAGCC TTTACATGAT TCAGAAATTG CTGAACAGGC TGTCTGTGAT
    CCTGGGAACG TATGTGTGAC CGGAGCCCCA AAACACCCGA TCTCTGAAGA ACTGGAAGCT
    CCAGTGAAAG ACAGCCACCT GATCCCTACG CCTCAAGCCC CCAGTATTGC CTTCCCCCTA
    GCCAACCCAC CTGTGGCTCC ACACCCTAGA GAAAAGATTA TAACAATAGA GGAGACTCAT
    GAAGACTTAA AAAAACAGTA CATATTTCAG TTGTCATCTC TGAATCCTCA AGAACGTATT
    GATTATTGTC ATCTGATTGA AAAACTAGGT GGATCAGTGA TTGAGAAGCA GTGCTTTGAT
    CCCAGCTGTA CACACATTGT TGTGGGGCAT CCACTTCGCA ACGAGAAGTA TTTAGCCTCC
    GTGGCAGCGG GAAAGTGGGT ACTCCATCGC TCCTACCTCG AAGCTTGCAG GACTGCTGGG
    CGCTTCATGT CGGAAGAAGA CTATGAGTGG GGAAGTAGTT CCATACTTGA TGTTTTGACT
    GGAATCAATG TACAGCAACG AAAACTAGCA CTTGCAGCAA TGAGATGGAG GAAAAAAATC
    CAGCAAAGAC AAGAATCAGG CATTACTGAG GGGGCATTTA GCGGGTGGAA AGTTATTTTA
    CATGTTGATC AGTCCCGAGA ATCAGGATTC AAACGCCTAC TTCAGTCAGG AGGAGCAAAG
    GTGCTGCCTG GTTATTCTGT ATCTTTATTT AAAGAAGCTA CACATCTCTT TTCTGACTTT
    AATAAACAGA AACCAGATGA CTCAGGAGTT AATATAGCAG AAGCTGCTGC CCAGAATGTA
    TACTGCTTGA AAACAGAATA CATTGCTGAT TTTCTTATGC AGGAATCCCC TCCTCAAGTA
    GAAAATTACT GTCTACCAGA AGCTGCTTCA TTTCTTCAGA ATAAAAAGGA ACCTGGGCCT
    GGATTCTCAC AAAAGAGGAA AGCTCCTGCA GAAAAAAATA AAATCAAACG ACCCAGAGTA
    CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16052
    Clone ID Related Accession (Same CDS sequence) XM_005201960.3 , XM_005201960.4
    Accession Version XM_005201960.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103822.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201960.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAAAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCT 
    GAATGTTTTT TTGAAGCTCT TGAGTCAATA AAAGAGTTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAAATA AAGGAGAATG ATAGATCACT GTATATCTGT
    GACCCCTTTA GTGGTGATGC CTTTGATCAT CTCAAAAAGC TTGGTTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC TGAACATCCA
    GTTTATAACA TGGTTATGTC TGATGTAACC GTGTCCTGTA CAAGCCTGGA AAAAGACAGA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GCGTATACAG AGACCTTAAC
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAG
    GAGAAGAAAA TAGCTAGATA TACTGATATA AACATGGAAG ACTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTACCTTAG ACAGGAAATC AGTTCAGCAG
    CTAACAGTAA AGCATGGAGG ACAGTACATG GGACAGTTGA AAATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CGCAGTGGTT TTTTGACAGT GTTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ATCAGAAGCA AAGAGTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCA ACACAATTGA TAATGTCAGC CATATTTCCA ACATCAATGC AAGCTGCATA
    AATGAATCAA TATGTAATTC AGTTAATAGC AAACTGGAAC CTACAATTGA AAATCTGGAA
    AATTTGGATA TCAGTGCATT TCAGGCACCT GAAGATTTAT TAGATGGTTG TCGGATCTAT
    CTTTGTGGTT TTAATGGCAG AAAGCTAGAT AAACTGAGAA GACTTATTAA CAGTGGAGGT
    GGAGTTCGTT TTAATCAGCT CAATGAAGAT GTAACTCATG TTATTGTGGG AGATGATGAT
    GATGAATTGA AGCAGTTTTG GGATAAATCA GCCCACAGGC CGCATGTAGT GGGAGCAAAA
    TGGTTGCTAG AGTGTTTTAG TAAAGGCTAC ATGCTTCCTG AAGAGCCATA CATCCACGTT
    AACTACCAGC CAGTGGAAAT TCCAGTTTCA GATCCACCTG AAAGTAAAAC AGCCCTTTTG
    AAAAGGAAGA ACAACGGCTT CTCTAAGAGT GACTTTGTTC CTGAGGAGAA GCATGAGCAA
    GCTGATGAAG ATCTGCTCTC TCAGTATCAG ACTAATAACC CCACAGTAGT TGAAGTTGCC
    AAGTCTGAAG TTGGGCTCTG TAGTGACTCT ACTCATGTGG AGCCCCGTAA TGATTCTACT
    CATATTTCTC TGCAAGAACA CCAGTCTTCC ATCAGTCATT GCCTCCCTGA TGTTTCTACA
    ATTACCGAAG AAGGTTTATT TAGCCAAAAG AGTTTCCTAG TTTTGGGGTT TAGTAATGAA
    AATGAATCTA ATATTGCAAT CATCATAAGA GAAAATGCTG GAAAAATTGT GTCCCTTCAG
    AGCAGAGTTG TTGCTGATTA TGCTGTGGTT CCTCTGCTGG GATGTGAAGT ACAGGCAACT
    GTGGGAGAAG TTGTAACAAA CACATGGCTG GTAATGTGTA TAGACTACCA GACTTTAATT
    GATCCAAAGT CAAATCCTCT CTTCACACCA GTTCCAGTTA TGGCAGGAAT GACTCCTTTA
    GAGGGTTGTG TTCTTTCATT CAGCCAATGT GTAGGAGCTG AAAAAGATTC ATTGACATTC
    TTAGCAAATC ACCTTGGAGC CAGTGTTCAA GAATACTTCG TTCGCAAGTC CAATGCAAAG
    AAAGGCATGT TTGCTAGTAC ACATCTTGTT TTGAAAGAGC CAGGTGGTTC TAAATATGAA
    GCTGCCAAGA AGTGGAATTT ACCAGCAGTC ACCATAGCTT GGCTGTTAGA GTCTGCTAGA
    TTGGGAAAGA GAGCAAATGA AAACCATTTC CTGATTGAAA ATTCATCTAA AGAAGAACAA
    AGTTTGGAAA CTGAAACAAC AAATGAGGTG AATCTAAATC CAGATACTCC AGAGCATCCT
    GTAACACATC TGGAAACTCA GAGAAAAACA GCCGTTACAC CTTTAGATAT GAACCGCTTT
    CAGAGTAAAG CTTTCCGCAC GGTGATGTCA CAGCACTCCG GACAGGCTTC AGTGTCCCCT
    TCCCCAGGAC AGTCACTTCA GAAGGAGCCC TCATTACACC TGGATACGCC GTCAAAGTTT
    CTGTCCAAGG ACAAACTGTT CAAGCCTTCC TTTGATGTGA AGGATGCTCT CGCAGCCTTG
    GAAACTCCAG GAGGTCCCAG CCAGCAGAAA AGGAAACTGA GTACACCACT CTCAGAAGTC
    ATCGTCAAAA ACCTGAAACT TGCTTTGGCA AATAGCTCTC GAAATGCTGT TGCTCTTTCT
    GCCAGCCCTC AACTGAAGGA TGCCCATTCA GAAAAGGAAG ATGCTCCGAA GCCACTTGAG
    AACGTTGTGG TGTGTGTTAG TAAGAAACTC AGTAAGAAGC AGAGTGAACT AAATGGGATT
    GCGGCCTCTC TAGGTGCAGA CTACAGGTGG AGTTTTGATG AGACAGTGAC TCATTTCATC
    TATCAAGGAC GACCAAATGA CACTAATCGG GAGTATAAAT CTGTAAAAGA AAGAGGAGTA
    CACATTGTTT CAGAGCACTG GCTTTTAGAG TGTGCCCAGG AGTATAAACA TCTTCCCGAA
    TCTCTTTATC CACATACTTA CAATCCCAAA ATGAGCTTGG ACATCAGTGC AGTGCAAGAT
    GGCCGACTCT GTAATAACCG ACTACTTTCA GCTGGCTCTG CAATGAGGGA TGATGAGCCA
    GATCATATGC CTCTAGAAGA AAATGATATA GACAATATGA CCACCAGTAA TAAAGAACTA
    ACAACATCAA ATGGAAATGG GAGGAATGAC TCTAAAGGAG CTCTGACACA GACTTTGGAG
    ATGAGAGAGA ACTTTCAAAA GCAGCTTCAG GAGATAATGT CTGCAACTTC AATAGTGAAA
    CCCCAAGGGC AGAGGACTTC CCTCTCAAGA AGTGGTTGTA ACAGTACCTC TTCAACTCCT
    GACAGCACCC GCTCTGCCCG CAGTGGACGA AGTAGAGTCC TAGAGGCACT GAGGCAGTCT
    CGTCAAACAG TACCTGATGT CAACACAGAG CCCTCCCAAA ATGAACAGAT CATTTGGGAT
    GACCCTACAG CCAGGGAGGA GAGAGCAAGA CTTGCCAGCA ATTTGCAGTG GCCTAGTTGT
    CCCACACAAT ACTCTGAGCT TCAGGTTGAT GTTAAAAAAC TGGAGGATTC TCCTTTCCAG
    GAGCCTTTAC ATGATTCAGA AATTGCTGAA CAGGCTGTCT GTGATCCTGG GAACGTATGT
    GTGACCGGAG CCCCAAAACA CCCGATCTCT GAAGAACTGG AAGCTCCAGT GAAAGACAGC
    CACCTGATCC CTACGCCTCA AGCCCCCAGT ATTGCCTTCC CCCTAGCCAA CCCACCTGTG
    GCTCCACACC CTAGAGAAAA GATTATAACA ATAGAGGAGA CTCATGAAGA CTTAAAAAAA
    CAGTACATAT TTCAGTTGTC ATCTCTGAAT CCTCAAGAAC GTATTGATTA TTGTCATCTG
    ATTGAAAAAC TAGGTGGATC AGTGATTGAG AAGCAGTGCT TTGATCCCAG CTGTACACAC
    ATTGTTGTGG GGCATCCACT TCGCAACGAG AAGTATTTAG CCTCCGTGGC AGCGGGAAAG
    TGGGTACTCC ATCGCTCCTA CCTCGAAGCT TGCAGGACTG CTGGGCGCTT CATGTCGGAA
    GAAGACTATG AGTGGGGAAG TAGTTCCATA CTTGATGTTT TGACTGGAAT CAATGTACAG
    CAACGAAAAC TAGCACTTGC AGCAATGAGA TGGAGGAAAA AAATCCAGCA AAGACAAGAA
    TCAGGCATTA CTGAGGGGGC ATTTAGCGGG TGGAAAGTTA TTTTACATGT TGATCAGTCC
    CGAGAATCAG GATTCAAACG CCTACTTCAG TCAGGAGGAG CAAAGGTGCT GCCTGGTTAT
    TCTGTATCTT TATTTAAAGA AGCTACACAT CTCTTTTCTG ACTTTAATAA ACAGAAACCA
    GATGACTCAG GAGTTAATAT AGCAGAAGCT GCTGCCCAGA ATGTATACTG CTTGAAAACA
    GAATACATTG CTGATTTTCT TATGCAGGAA TCCCCTCCTC AAGTAGAAAA TTACTGTCTA
    CCAGAAGCTG CTTCATTTCT TCAGAATAAA AAGGAACCTG GGCCTGGATT CTCACAAAAG
    AGGAAAGCTC CTGCAGAAAA AAATAAAATC AAACGACCCA GAGTACAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202017.1
    CDS170..4720
    Translation

    Target ORF information:

    RefSeq Version XM_005201960.3
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201960.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAAAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCT 
    GAATGTTTTT TTGAAGCTCT TGAGTCAATA AAAGAGTTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAAATA AAGGAGAATG ATAGATCACT GTATATCTGT
    GACCCCTTTA GTGGTGATGC CTTTGATCAT CTCAAAAAGC TTGGTTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC TGAACATCCA
    GTTTATAACA TGGTTATGTC TGATGTAACC GTGTCCTGTA CAAGCCTGGA AAAAGACAGA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GCGTATACAG AGACCTTAAC
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAG
    GAGAAGAAAA TAGCTAGATA TACTGATATA AACATGGAAG ACTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTACCTTAG ACAGGAAATC AGTTCAGCAG
    CTAACAGTAA AGCATGGAGG ACAGTACATG GGACAGTTGA AAATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CGCAGTGGTT TTTTGACAGT GTTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ATCAGAAGCA AAGAGTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCA ACACAATTGA TAATGTCAGC CATATTTCCA ACATCAATGC AAGCTGCATA
    AATGAATCAA TATGTAATTC AGTTAATAGC AAACTGGAAC CTACAATTGA AAATCTGGAA
    AATTTGGATA TCAGTGCATT TCAGGCACCT GAAGATTTAT TAGATGGTTG TCGGATCTAT
    CTTTGTGGTT TTAATGGCAG AAAGCTAGAT AAACTGAGAA GACTTATTAA CAGTGGAGGT
    GGAGTTCGTT TTAATCAGCT CAATGAAGAT GTAACTCATG TTATTGTGGG AGATGATGAT
    GATGAATTGA AGCAGTTTTG GGATAAATCA GCCCACAGGC CGCATGTAGT GGGAGCAAAA
    TGGTTGCTAG AGTGTTTTAG TAAAGGCTAC ATGCTTCCTG AAGAGCCATA CATCCACGTT
    AACTACCAGC CAGTGGAAAT TCCAGTTTCA GATCCACCTG AAAGTAAAAC AGCCCTTTTG
    AAAAGGAAGA ACAACGGCTT CTCTAAGAGT GACTTTGTTC CTGAGGAGAA GCATGAGCAA
    GCTGATGAAG ATCTGCTCTC TCAGTATCAG ACTAATAACC CCACAGTAGT TGAAGTTGCC
    AAGTCTGAAG TTGGGCTCTG TAGTGACTCT ACTCATGTGG AGCCCCGTAA TGATTCTACT
    CATATTTCTC TGCAAGAACA CCAGTCTTCC ATCAGTCATT GCCTCCCTGA TGTTTCTACA
    ATTACCGAAG AAGGTTTATT TAGCCAAAAG AGTTTCCTAG TTTTGGGGTT TAGTAATGAA
    AATGAATCTA ATATTGCAAT CATCATAAGA GAAAATGCTG GAAAAATTGT GTCCCTTCAG
    AGCAGAGTTG TTGCTGATTA TGCTGTGGTT CCTCTGCTGG GATGTGAAGT ACAGGCAACT
    GTGGGAGAAG TTGTAACAAA CACATGGCTG GTAATGTGTA TAGACTACCA GACTTTAATT
    GATCCAAAGT CAAATCCTCT CTTCACACCA GTTCCAGTTA TGGCAGGAAT GACTCCTTTA
    GAGGGTTGTG TTCTTTCATT CAGCCAATGT GTAGGAGCTG AAAAAGATTC ATTGACATTC
    TTAGCAAATC ACCTTGGAGC CAGTGTTCAA GAATACTTCG TTCGCAAGTC CAATGCAAAG
    AAAGGCATGT TTGCTAGTAC ACATCTTGTT TTGAAAGAGC CAGGTGGTTC TAAATATGAA
    GCTGCCAAGA AGTGGAATTT ACCAGCAGTC ACCATAGCTT GGCTGTTAGA GTCTGCTAGA
    TTGGGAAAGA GAGCAAATGA AAACCATTTC CTGATTGAAA ATTCATCTAA AGAAGAACAA
    AGTTTGGAAA CTGAAACAAC AAATGAGGTG AATCTAAATC CAGATACTCC AGAGCATCCT
    GTAACACATC TGGAAACTCA GAGAAAAACA GCCGTTACAC CTTTAGATAT GAACCGCTTT
    CAGAGTAAAG CTTTCCGCAC GGTGATGTCA CAGCACTCCG GACAGGCTTC AGTGTCCCCT
    TCCCCAGGAC AGTCACTTCA GAAGGAGCCC TCATTACACC TGGATACGCC GTCAAAGTTT
    CTGTCCAAGG ACAAACTGTT CAAGCCTTCC TTTGATGTGA AGGATGCTCT CGCAGCCTTG
    GAAACTCCAG GAGGTCCCAG CCAGCAGAAA AGGAAACTGA GTACACCACT CTCAGAAGTC
    ATCGTCAAAA ACCTGAAACT TGCTTTGGCA AATAGCTCTC GAAATGCTGT TGCTCTTTCT
    GCCAGCCCTC AACTGAAGGA TGCCCATTCA GAAAAGGAAG ATGCTCCGAA GCCACTTGAG
    AACGTTGTGG TGTGTGTTAG TAAGAAACTC AGTAAGAAGC AGAGTGAACT AAATGGGATT
    GCGGCCTCTC TAGGTGCAGA CTACAGGTGG AGTTTTGATG AGACAGTGAC TCATTTCATC
    TATCAAGGAC GACCAAATGA CACTAATCGG GAGTATAAAT CTGTAAAAGA AAGAGGAGTA
    CACATTGTTT CAGAGCACTG GCTTTTAGAG TGTGCCCAGG AGTATAAACA TCTTCCCGAA
    TCTCTTTATC CACATACTTA CAATCCCAAA ATGAGCTTGG ACATCAGTGC AGTGCAAGAT
    GGCCGACTCT GTAATAACCG ACTACTTTCA GCTGGCTCTG CAATGAGGGA TGATGAGCCA
    GATCATATGC CTCTAGAAGA AAATGATATA GACAATATGA CCACCAGTAA TAAAGAACTA
    ACAACATCAA ATGGAAATGG GAGGAATGAC TCTAAAGGAG CTCTGACACA GACTTTGGAG
    ATGAGAGAGA ACTTTCAAAA GCAGCTTCAG GAGATAATGT CTGCAACTTC AATAGTGAAA
    CCCCAAGGGC AGAGGACTTC CCTCTCAAGA AGTGGTTGTA ACAGTACCTC TTCAACTCCT
    GACAGCACCC GCTCTGCCCG CAGTGGACGA AGTAGAGTCC TAGAGGCACT GAGGCAGTCT
    CGTCAAACAG TACCTGATGT CAACACAGAG CCCTCCCAAA ATGAACAGAT CATTTGGGAT
    GACCCTACAG CCAGGGAGGA GAGAGCAAGA CTTGCCAGCA ATTTGCAGTG GCCTAGTTGT
    CCCACACAAT ACTCTGAGCT TCAGGTTGAT GTTAAAAAAC TGGAGGATTC TCCTTTCCAG
    GAGCCTTTAC ATGATTCAGA AATTGCTGAA CAGGCTGTCT GTGATCCTGG GAACGTATGT
    GTGACCGGAG CCCCAAAACA CCCGATCTCT GAAGAACTGG AAGCTCCAGT GAAAGACAGC
    CACCTGATCC CTACGCCTCA AGCCCCCAGT ATTGCCTTCC CCCTAGCCAA CCCACCTGTG
    GCTCCACACC CTAGAGAAAA GATTATAACA ATAGAGGAGA CTCATGAAGA CTTAAAAAAA
    CAGTACATAT TTCAGTTGTC ATCTCTGAAT CCTCAAGAAC GTATTGATTA TTGTCATCTG
    ATTGAAAAAC TAGGTGGATC AGTGATTGAG AAGCAGTGCT TTGATCCCAG CTGTACACAC
    ATTGTTGTGG GGCATCCACT TCGCAACGAG AAGTATTTAG CCTCCGTGGC AGCGGGAAAG
    TGGGTACTCC ATCGCTCCTA CCTCGAAGCT TGCAGGACTG CTGGGCGCTT CATGTCGGAA
    GAAGACTATG AGTGGGGAAG TAGTTCCATA CTTGATGTTT TGACTGGAAT CAATGTACAG
    CAACGAAAAC TAGCACTTGC AGCAATGAGA TGGAGGAAAA AAATCCAGCA AAGACAAGAA
    TCAGGCATTA CTGAGGGGGC ATTTAGCGGG TGGAAAGTTA TTTTACATGT TGATCAGTCC
    CGAGAATCAG GATTCAAACG CCTACTTCAG TCAGGAGGAG CAAAGGTGCT GCCTGGTTAT
    TCTGTATCTT TATTTAAAGA AGCTACACAT CTCTTTTCTG ACTTTAATAA ACAGAAACCA
    GATGACTCAG GAGTTAATAT AGCAGAAGCT GCTGCCCAGA ATGTATACTG CTTGAAAACA
    GAATACATTG CTGATTTTCT TATGCAGGAA TCCCCTCCTC AAGTAGAAAA TTACTGTCTA
    CCAGAAGCTG CTTCATTTCT TCAGAATAAA AAGGAACCTG GGCCTGGATT CTCACAAAAG
    AGGAAAGCTC CTGCAGAAAA AAATAAAATC AAACGACCCA GAGTACAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16052
    Clone ID Related Accession (Same CDS sequence) XM_005201960.3 , XM_005201960.4
    Accession Version XM_005201960.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201960.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAAAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCT 
    GAATGTTTTT TTGAAGCTCT TGAGTCAATA AAAGAGTTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAAATA AAGGAGAATG ATAGATCACT GTATATCTGT
    GACCCCTTTA GTGGTGATGC CTTTGATCAT CTCAAAAAGC TTGGTTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC TGAACATCCA
    GTTTATAACA TGGTTATGTC TGATGTAACC GTGTCCTGTA CAAGCCTGGA AAAAGACAGA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GCGTATACAG AGACCTTAAC
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAG
    GAGAAGAAAA TAGCTAGATA TACTGATATA AACATGGAAG ACTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTACCTTAG ACAGGAAATC AGTTCAGCAG
    CTAACAGTAA AGCATGGAGG ACAGTACATG GGACAGTTGA AAATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CGCAGTGGTT TTTTGACAGT GTTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ATCAGAAGCA AAGAGTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCA ACACAATTGA TAATGTCAGC CATATTTCCA ACATCAATGC AAGCTGCATA
    AATGAATCAA TATGTAATTC AGTTAATAGC AAACTGGAAC CTACAATTGA AAATCTGGAA
    AATTTGGATA TCAGTGCATT TCAGGCACCT GAAGATTTAT TAGATGGTTG TCGGATCTAT
    CTTTGTGGTT TTAATGGCAG AAAGCTAGAT AAACTGAGAA GACTTATTAA CAGTGGAGGT
    GGAGTTCGTT TTAATCAGCT CAATGAAGAT GTAACTCATG TTATTGTGGG AGATGATGAT
    GATGAATTGA AGCAGTTTTG GGATAAATCA GCCCACAGGC CGCATGTAGT GGGAGCAAAA
    TGGTTGCTAG AGTGTTTTAG TAAAGGCTAC ATGCTTCCTG AAGAGCCATA CATCCACGTT
    AACTACCAGC CAGTGGAAAT TCCAGTTTCA GATCCACCTG AAAGTAAAAC AGCCCTTTTG
    AAAAGGAAGA ACAACGGCTT CTCTAAGAGT GACTTTGTTC CTGAGGAGAA GCATGAGCAA
    GCTGATGAAG ATCTGCTCTC TCAGTATCAG ACTAATAACC CCACAGTAGT TGAAGTTGCC
    AAGTCTGAAG TTGGGCTCTG TAGTGACTCT ACTCATGTGG AGCCCCGTAA TGATTCTACT
    CATATTTCTC TGCAAGAACA CCAGTCTTCC ATCAGTCATT GCCTCCCTGA TGTTTCTACA
    ATTACCGAAG AAGGTTTATT TAGCCAAAAG AGTTTCCTAG TTTTGGGGTT TAGTAATGAA
    AATGAATCTA ATATTGCAAT CATCATAAGA GAAAATGCTG GAAAAATTGT GTCCCTTCAG
    AGCAGAGTTG TTGCTGATTA TGCTGTGGTT CCTCTGCTGG GATGTGAAGT ACAGGCAACT
    GTGGGAGAAG TTGTAACAAA CACATGGCTG GTAATGTGTA TAGACTACCA GACTTTAATT
    GATCCAAAGT CAAATCCTCT CTTCACACCA GTTCCAGTTA TGGCAGGAAT GACTCCTTTA
    GAGGGTTGTG TTCTTTCATT CAGCCAATGT GTAGGAGCTG AAAAAGATTC ATTGACATTC
    TTAGCAAATC ACCTTGGAGC CAGTGTTCAA GAATACTTCG TTCGCAAGTC CAATGCAAAG
    AAAGGCATGT TTGCTAGTAC ACATCTTGTT TTGAAAGAGC CAGGTGGTTC TAAATATGAA
    GCTGCCAAGA AGTGGAATTT ACCAGCAGTC ACCATAGCTT GGCTGTTAGA GTCTGCTAGA
    TTGGGAAAGA GAGCAAATGA AAACCATTTC CTGATTGAAA ATTCATCTAA AGAAGAACAA
    AGTTTGGAAA CTGAAACAAC AAATGAGGTG AATCTAAATC CAGATACTCC AGAGCATCCT
    GTAACACATC TGGAAACTCA GAGAAAAACA GCCGTTACAC CTTTAGATAT GAACCGCTTT
    CAGAGTAAAG CTTTCCGCAC GGTGATGTCA CAGCACTCCG GACAGGCTTC AGTGTCCCCT
    TCCCCAGGAC AGTCACTTCA GAAGGAGCCC TCATTACACC TGGATACGCC GTCAAAGTTT
    CTGTCCAAGG ACAAACTGTT CAAGCCTTCC TTTGATGTGA AGGATGCTCT CGCAGCCTTG
    GAAACTCCAG GAGGTCCCAG CCAGCAGAAA AGGAAACTGA GTACACCACT CTCAGAAGTC
    ATCGTCAAAA ACCTGAAACT TGCTTTGGCA AATAGCTCTC GAAATGCTGT TGCTCTTTCT
    GCCAGCCCTC AACTGAAGGA TGCCCATTCA GAAAAGGAAG ATGCTCCGAA GCCACTTGAG
    AACGTTGTGG TGTGTGTTAG TAAGAAACTC AGTAAGAAGC AGAGTGAACT AAATGGGATT
    GCGGCCTCTC TAGGTGCAGA CTACAGGTGG AGTTTTGATG AGACAGTGAC TCATTTCATC
    TATCAAGGAC GACCAAATGA CACTAATCGG GAGTATAAAT CTGTAAAAGA AAGAGGAGTA
    CACATTGTTT CAGAGCACTG GCTTTTAGAG TGTGCCCAGG AGTATAAACA TCTTCCCGAA
    TCTCTTTATC CACATACTTA CAATCCCAAA ATGAGCTTGG ACATCAGTGC AGTGCAAGAT
    GGCCGACTCT GTAATAACCG ACTACTTTCA GCTGGCTCTG CAATGAGGGA TGATGAGCCA
    GATCATATGC CTCTAGAAGA AAATGATATA GACAATATGA CCACCAGTAA TAAAGAACTA
    ACAACATCAA ATGGAAATGG GAGGAATGAC TCTAAAGGAG CTCTGACACA GACTTTGGAG
    ATGAGAGAGA ACTTTCAAAA GCAGCTTCAG GAGATAATGT CTGCAACTTC AATAGTGAAA
    CCCCAAGGGC AGAGGACTTC CCTCTCAAGA AGTGGTTGTA ACAGTACCTC TTCAACTCCT
    GACAGCACCC GCTCTGCCCG CAGTGGACGA AGTAGAGTCC TAGAGGCACT GAGGCAGTCT
    CGTCAAACAG TACCTGATGT CAACACAGAG CCCTCCCAAA ATGAACAGAT CATTTGGGAT
    GACCCTACAG CCAGGGAGGA GAGAGCAAGA CTTGCCAGCA ATTTGCAGTG GCCTAGTTGT
    CCCACACAAT ACTCTGAGCT TCAGGTTGAT GTTAAAAAAC TGGAGGATTC TCCTTTCCAG
    GAGCCTTTAC ATGATTCAGA AATTGCTGAA CAGGCTGTCT GTGATCCTGG GAACGTATGT
    GTGACCGGAG CCCCAAAACA CCCGATCTCT GAAGAACTGG AAGCTCCAGT GAAAGACAGC
    CACCTGATCC CTACGCCTCA AGCCCCCAGT ATTGCCTTCC CCCTAGCCAA CCCACCTGTG
    GCTCCACACC CTAGAGAAAA GATTATAACA ATAGAGGAGA CTCATGAAGA CTTAAAAAAA
    CAGTACATAT TTCAGTTGTC ATCTCTGAAT CCTCAAGAAC GTATTGATTA TTGTCATCTG
    ATTGAAAAAC TAGGTGGATC AGTGATTGAG AAGCAGTGCT TTGATCCCAG CTGTACACAC
    ATTGTTGTGG GGCATCCACT TCGCAACGAG AAGTATTTAG CCTCCGTGGC AGCGGGAAAG
    TGGGTACTCC ATCGCTCCTA CCTCGAAGCT TGCAGGACTG CTGGGCGCTT CATGTCGGAA
    GAAGACTATG AGTGGGGAAG TAGTTCCATA CTTGATGTTT TGACTGGAAT CAATGTACAG
    CAACGAAAAC TAGCACTTGC AGCAATGAGA TGGAGGAAAA AAATCCAGCA AAGACAAGAA
    TCAGGCATTA CTGAGGGGGC ATTTAGCGGG TGGAAAGTTA TTTTACATGT TGATCAGTCC
    CGAGAATCAG GATTCAAACG CCTACTTCAG TCAGGAGGAG CAAAGGTGCT GCCTGGTTAT
    TCTGTATCTT TATTTAAAGA AGCTACACAT CTCTTTTCTG ACTTTAATAA ACAGAAACCA
    GATGACTCAG GAGTTAATAT AGCAGAAGCT GCTGCCCAGA ATGTATACTG CTTGAAAACA
    GAATACATTG CTGATTTTCT TATGCAGGAA TCCCCTCCTC AAGTAGAAAA TTACTGTCTA
    CCAGAAGCTG CTTCATTTCT TCAGAATAAA AAGGAACCTG GGCCTGGATT CTCACAAAAG
    AGGAAAGCTC CTGCAGAAAA AAATAAAATC AAACGACCCA GAGTACAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202017.1
    CDS170..4720
    Translation

    Target ORF information:

    RefSeq Version XM_005201960.4
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201960.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAAAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCT 
    GAATGTTTTT TTGAAGCTCT TGAGTCAATA AAAGAGTTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAAATA AAGGAGAATG ATAGATCACT GTATATCTGT
    GACCCCTTTA GTGGTGATGC CTTTGATCAT CTCAAAAAGC TTGGTTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC TGAACATCCA
    GTTTATAACA TGGTTATGTC TGATGTAACC GTGTCCTGTA CAAGCCTGGA AAAAGACAGA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGTGGAC GCGTATACAG AGACCTTAAC
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATACTT AGTTGCTGCA
    AACCTAAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAG
    GAGAAGAAAA TAGCTAGATA TACTGATATA AACATGGAAG ACTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTACCTTAG ACAGGAAATC AGTTCAGCAG
    CTAACAGTAA AGCATGGAGG ACAGTACATG GGACAGTTGA AAATGAATGA GTGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAAAAA TATGAATGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CGCAGTGGTT TTTTGACAGT GTTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAGCCTAG ATCAGAAGCA AAGAGTATGC CTGATACTTC AACTCCTACT
    GGCCAGATCA ACACAATTGA TAATGTCAGC CATATTTCCA ACATCAATGC AAGCTGCATA
    AATGAATCAA TATGTAATTC AGTTAATAGC AAACTGGAAC CTACAATTGA AAATCTGGAA
    AATTTGGATA TCAGTGCATT TCAGGCACCT GAAGATTTAT TAGATGGTTG TCGGATCTAT
    CTTTGTGGTT TTAATGGCAG AAAGCTAGAT AAACTGAGAA GACTTATTAA CAGTGGAGGT
    GGAGTTCGTT TTAATCAGCT CAATGAAGAT GTAACTCATG TTATTGTGGG AGATGATGAT
    GATGAATTGA AGCAGTTTTG GGATAAATCA GCCCACAGGC CGCATGTAGT GGGAGCAAAA
    TGGTTGCTAG AGTGTTTTAG TAAAGGCTAC ATGCTTCCTG AAGAGCCATA CATCCACGTT
    AACTACCAGC CAGTGGAAAT TCCAGTTTCA GATCCACCTG AAAGTAAAAC AGCCCTTTTG
    AAAAGGAAGA ACAACGGCTT CTCTAAGAGT GACTTTGTTC CTGAGGAGAA GCATGAGCAA
    GCTGATGAAG ATCTGCTCTC TCAGTATCAG ACTAATAACC CCACAGTAGT TGAAGTTGCC
    AAGTCTGAAG TTGGGCTCTG TAGTGACTCT ACTCATGTGG AGCCCCGTAA TGATTCTACT
    CATATTTCTC TGCAAGAACA CCAGTCTTCC ATCAGTCATT GCCTCCCTGA TGTTTCTACA
    ATTACCGAAG AAGGTTTATT TAGCCAAAAG AGTTTCCTAG TTTTGGGGTT TAGTAATGAA
    AATGAATCTA ATATTGCAAT CATCATAAGA GAAAATGCTG GAAAAATTGT GTCCCTTCAG
    AGCAGAGTTG TTGCTGATTA TGCTGTGGTT CCTCTGCTGG GATGTGAAGT ACAGGCAACT
    GTGGGAGAAG TTGTAACAAA CACATGGCTG GTAATGTGTA TAGACTACCA GACTTTAATT
    GATCCAAAGT CAAATCCTCT CTTCACACCA GTTCCAGTTA TGGCAGGAAT GACTCCTTTA
    GAGGGTTGTG TTCTTTCATT CAGCCAATGT GTAGGAGCTG AAAAAGATTC ATTGACATTC
    TTAGCAAATC ACCTTGGAGC CAGTGTTCAA GAATACTTCG TTCGCAAGTC CAATGCAAAG
    AAAGGCATGT TTGCTAGTAC ACATCTTGTT TTGAAAGAGC CAGGTGGTTC TAAATATGAA
    GCTGCCAAGA AGTGGAATTT ACCAGCAGTC ACCATAGCTT GGCTGTTAGA GTCTGCTAGA
    TTGGGAAAGA GAGCAAATGA AAACCATTTC CTGATTGAAA ATTCATCTAA AGAAGAACAA
    AGTTTGGAAA CTGAAACAAC AAATGAGGTG AATCTAAATC CAGATACTCC AGAGCATCCT
    GTAACACATC TGGAAACTCA GAGAAAAACA GCCGTTACAC CTTTAGATAT GAACCGCTTT
    CAGAGTAAAG CTTTCCGCAC GGTGATGTCA CAGCACTCCG GACAGGCTTC AGTGTCCCCT
    TCCCCAGGAC AGTCACTTCA GAAGGAGCCC TCATTACACC TGGATACGCC GTCAAAGTTT
    CTGTCCAAGG ACAAACTGTT CAAGCCTTCC TTTGATGTGA AGGATGCTCT CGCAGCCTTG
    GAAACTCCAG GAGGTCCCAG CCAGCAGAAA AGGAAACTGA GTACACCACT CTCAGAAGTC
    ATCGTCAAAA ACCTGAAACT TGCTTTGGCA AATAGCTCTC GAAATGCTGT TGCTCTTTCT
    GCCAGCCCTC AACTGAAGGA TGCCCATTCA GAAAAGGAAG ATGCTCCGAA GCCACTTGAG
    AACGTTGTGG TGTGTGTTAG TAAGAAACTC AGTAAGAAGC AGAGTGAACT AAATGGGATT
    GCGGCCTCTC TAGGTGCAGA CTACAGGTGG AGTTTTGATG AGACAGTGAC TCATTTCATC
    TATCAAGGAC GACCAAATGA CACTAATCGG GAGTATAAAT CTGTAAAAGA AAGAGGAGTA
    CACATTGTTT CAGAGCACTG GCTTTTAGAG TGTGCCCAGG AGTATAAACA TCTTCCCGAA
    TCTCTTTATC CACATACTTA CAATCCCAAA ATGAGCTTGG ACATCAGTGC AGTGCAAGAT
    GGCCGACTCT GTAATAACCG ACTACTTTCA GCTGGCTCTG CAATGAGGGA TGATGAGCCA
    GATCATATGC CTCTAGAAGA AAATGATATA GACAATATGA CCACCAGTAA TAAAGAACTA
    ACAACATCAA ATGGAAATGG GAGGAATGAC TCTAAAGGAG CTCTGACACA GACTTTGGAG
    ATGAGAGAGA ACTTTCAAAA GCAGCTTCAG GAGATAATGT CTGCAACTTC AATAGTGAAA
    CCCCAAGGGC AGAGGACTTC CCTCTCAAGA AGTGGTTGTA ACAGTACCTC TTCAACTCCT
    GACAGCACCC GCTCTGCCCG CAGTGGACGA AGTAGAGTCC TAGAGGCACT GAGGCAGTCT
    CGTCAAACAG TACCTGATGT CAACACAGAG CCCTCCCAAA ATGAACAGAT CATTTGGGAT
    GACCCTACAG CCAGGGAGGA GAGAGCAAGA CTTGCCAGCA ATTTGCAGTG GCCTAGTTGT
    CCCACACAAT ACTCTGAGCT TCAGGTTGAT GTTAAAAAAC TGGAGGATTC TCCTTTCCAG
    GAGCCTTTAC ATGATTCAGA AATTGCTGAA CAGGCTGTCT GTGATCCTGG GAACGTATGT
    GTGACCGGAG CCCCAAAACA CCCGATCTCT GAAGAACTGG AAGCTCCAGT GAAAGACAGC
    CACCTGATCC CTACGCCTCA AGCCCCCAGT ATTGCCTTCC CCCTAGCCAA CCCACCTGTG
    GCTCCACACC CTAGAGAAAA GATTATAACA ATAGAGGAGA CTCATGAAGA CTTAAAAAAA
    CAGTACATAT TTCAGTTGTC ATCTCTGAAT CCTCAAGAAC GTATTGATTA TTGTCATCTG
    ATTGAAAAAC TAGGTGGATC AGTGATTGAG AAGCAGTGCT TTGATCCCAG CTGTACACAC
    ATTGTTGTGG GGCATCCACT TCGCAACGAG AAGTATTTAG CCTCCGTGGC AGCGGGAAAG
    TGGGTACTCC ATCGCTCCTA CCTCGAAGCT TGCAGGACTG CTGGGCGCTT CATGTCGGAA
    GAAGACTATG AGTGGGGAAG TAGTTCCATA CTTGATGTTT TGACTGGAAT CAATGTACAG
    CAACGAAAAC TAGCACTTGC AGCAATGAGA TGGAGGAAAA AAATCCAGCA AAGACAAGAA
    TCAGGCATTA CTGAGGGGGC ATTTAGCGGG TGGAAAGTTA TTTTACATGT TGATCAGTCC
    CGAGAATCAG GATTCAAACG CCTACTTCAG TCAGGAGGAG CAAAGGTGCT GCCTGGTTAT
    TCTGTATCTT TATTTAAAGA AGCTACACAT CTCTTTTCTG ACTTTAATAA ACAGAAACCA
    GATGACTCAG GAGTTAATAT AGCAGAAGCT GCTGCCCAGA ATGTATACTG CTTGAAAACA
    GAATACATTG CTGATTTTCT TATGCAGGAA TCCCCTCCTC AAGTAGAAAA TTACTGTCTA
    CCAGAAGCTG CTTCATTTCT TCAGAATAAA AAGGAACCTG GGCCTGGATT CTCACAAAAG
    AGGAAAGCTC CTGCAGAAAA AAATAAAATC AAACGACCCA GAGTACAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16053
    Clone ID Related Accession (Same CDS sequence) XM_005201961.3 , XM_024991344.1
    Accession Version XM_005201961.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-binding protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103822.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201961.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCACCACC AGCGATGTGT CCCAAGAGCT GAACATCCAG TTTATAACAT GGTTATGTCT 
    GATGTAACCG TGTCCTGTAC AAGCCTGGAA AAAGACAGAA GGGAAGAAGT TCATAAATAT
    GTACAAATGA TGGGTGGACG CGTATACAGA GACCTTAACG TATCAGTAAC TCACCTTATT
    GCAGGAGAAG TTGGTAGCAA AAAATACTTA GTTGCTGCAA ACCTAAAGAA ACCTATTTTG
    CTTCCCTCTT GGATAAAAAC ACTTTGGGAG AAGTCACAGG AGAAGAAAAT AGCTAGATAT
    ACTGATATAA ACATGGAAGA CTTCAAGTGT CCTATTTTTC TTGGTTGCAT AATCTGTGTG
    ACTGGCTTAT GTACCTTAGA CAGGAAATCA GTTCAGCAGC TAACAGTAAA GCATGGAGGA
    CAGTACATGG GACAGTTGAA AATGAATGAG TGTACACACC TCATTGTGCA AGAACCAAAA
    GGTCAAAAAT ATGAATGTGC CAAGAGATGG AATGTACACT GTGTGACCAC GCAGTGGTTT
    TTTGACAGTG TTGAGAAAGG TTTTTGTCAG GATGAATCCA TATACAAGAC AGAGCCTAGA
    TCAGAAGCAA AGAGTATGCC TGATACTTCA ACTCCTACTG GCCAGATCAA CACAATTGAT
    AGTCGTACTC TTTCAGATGT CAGCCATATT TCCAACATCA ATGCAAGCTG CATAAATGAA
    TCAATATGTA ATTCAGTTAA TAGCAAACTG GAACCTACAA TTGAAAATCT GGAAAATTTG
    GATATCAGTG CATTTCAGGC ACCTGAAGAT TTATTAGATG GTTGTCGGAT CTATCTTTGT
    GGTTTTAATG GCAGAAAGCT AGATAAACTG AGAAGACTTA TTAACAGTGG AGGTGGAGTT
    CGTTTTAATC AGCTCAATGA AGATGTAACT CATGTTATTG TGGGAGATGA TGATGATGAA
    TTGAAGCAGT TTTGGGATAA ATCAGCCCAC AGGCCGCATG TAGTGGGAGC AAAATGGTTG
    CTAGAGTGTT TTAGTAAAGG CTACATGCTT CCTGAAGAGC CATACATCCA CGTTAACTAC
    CAGCCAGTGG AAATTCCAGT TTCAGATCCA CCTGAAAGTA AAACAGCCCT TTTGAAAAGG
    AAGAACAACG GCTTCTCTAA GAGTGACTTT GTTCCTGAGG AGAAGCATGA GCAAGCTGAT
    GAAGATCTGC TCTCTCAGTA TCAGACTAAT AACCCCACAG TAGTTGAAGT TGCCAAGTCT
    GAAGTTGGGC TCTGTAGTGA CTCTACTCAT GTGGAGCCCC GTAATGATTC TACTCATATT
    TCTCTGCAAG AACACCAGTC TTCCATCAGT CATTGCCTCC CTGATGTTTC TACAATTACC
    GAAGAAGGTT TATTTAGCCA AAAGAGTTTC CTAGTTTTGG GGTTTAGTAA TGAAAATGAA
    TCTAATATTG CAATCATCAT AAGAGAAAAT GCTGGAAAAA TTGTGTCCCT TCAGAGCAGA
    GTTGTTGCTG ATTATGCTGT GGTTCCTCTG CTGGGATGTG AAGTACAGGC AACTGTGGGA
    GAAGTTGTAA CAAACACATG GCTGGTAATG TGTATAGACT ACCAGACTTT AATTGATCCA
    AAGTCAAATC CTCTCTTCAC ACCAGTTCCA GTTATGGCAG GAATGACTCC TTTAGAGGGT
    TGTGTTCTTT CATTCAGCCA ATGTGTAGGA GCTGAAAAAG ATTCATTGAC ATTCTTAGCA
    AATCACCTTG GAGCCAGTGT TCAAGAATAC TTCGTTCGCA AGTCCAATGC AAAGAAAGGC
    ATGTTTGCTA GTACACATCT TGTTTTGAAA GAGCCAGGTG GTTCTAAATA TGAAGCTGCC
    AAGAAGTGGA ATTTACCAGC AGTCACCATA GCTTGGCTGT TAGAGTCTGC TAGATTGGGA
    AAGAGAGCAA ATGAAAACCA TTTCCTGATT GAAAATTCAT CTAAAGAAGA ACAAAGTTTG
    GAAACTGAAA CAACAAATGA GGTGAATCTA AATCCAGATA CTCCAGAGCA TCCTGTAACA
    CATCTGGAAA CTCAGAGAAA AACAGCCGTT ACACCTTTAG ATATGAACCG CTTTCAGAGT
    AAAGCTTTCC GCACGGTGAT GTCACAGCAC TCCGGACAGG CTTCAGTGTC CCCTTCCCCA
    GGACAGTCAC TTCAGAAGGA GCCCTCATTA CACCTGGATA CGCCGTCAAA GTTTCTGTCC
    AAGGACAAAC TGTTCAAGCC TTCCTTTGAT GTGAAGGATG CTCTCGCAGC CTTGGAAACT
    CCAGGAGGTC CCAGCCAGCA GAAAAGGAAA CTGAGTACAC CACTCTCAGA AGTCATCGTC
    AAAAACCTGA AACTTGCTTT GGCAAATAGC TCTCGAAATG CTGTTGCTCT TTCTGCCAGC
    CCTCAACTGA AGGATGCCCA TTCAGAAAAG GAAGATGCTC CGAAGCCACT TGAGAACGTT
    GTGGTGTGTG TTAGTAAGAA ACTCAGTAAG AAGCAGAGTG AACTAAATGG GATTGCGGCC
    TCTCTAGGTG CAGACTACAG GTGGAGTTTT GATGAGACAG TGACTCATTT CATCTATCAA
    GGACGACCAA ATGACACTAA TCGGGAGTAT AAATCTGTAA AAGAAAGAGG AGTACACATT
    GTTTCAGAGC ACTGGCTTTT AGAGTGTGCC CAGGAGTATA AACATCTTCC CGAATCTCTT
    TATCCACATA CTTACAATCC CAAAATGAGC TTGGACATCA GTGCAGTGCA AGATGGCCGA
    CTCTGTAATA ACCGACTACT TTCAGCTGGC TCTGCAATGA GGGATGATGA GCCAGATCAT
    ATGCCTCTAG AAGAAAATGA TATAGACAAT ATGACCACCA GTAATAAAGA ACTAACAACA
    TCAAATGGAA ATGGGAGGAA TGACTCTAAA GGAGCTCTGA CACAGACTTT GGAGATGAGA
    GAGAACTTTC AAAAGCAGCT TCAGGAGATA ATGTCTGCAA CTTCAATAGT GAAACCCCAA
    GGGCAGAGGA CTTCCCTCTC AAGAAGTGGT TGTAACAGTA CCTCTTCAAC TCCTGACAGC
    ACCCGCTCTG CCCGCAGTGG ACGAAGTAGA GTCCTAGAGG CACTGAGGCA GTCTCGTCAA
    ACAGTACCTG ATGTCAACAC AGAGCCCTCC CAAAATGAAC AGATCATTTG GGATGACCCT
    ACAGCCAGGG AGGAGAGAGC AAGACTTGCC AGCAATTTGC AGTGGCCTAG TTGTCCCACA
    CAATACTCTG AGCTTCAGGT TGATGTTAAA AAACTGGAGG ATTCTCCTTT CCAGGAGCCT
    TTACATGATT CAGAAATTGC TGAACAGGCT GTCTGTGATC CTGGGAACGT ATGTGTGACC
    GGAGCCCCAA AACACCCGAT CTCTGAAGAA CTGGAAGCTC CAGTGAAAGA CAGCCACCTG
    ATCCCTACGC CTCAAGCCCC CAGTATTGCC TTCCCCCTAG CCAACCCACC TGTGGCTCCA
    CACCCTAGAG AAAAGATTAT AACAATAGAG GAGACTCATG AAGACTTAAA AAAACAGTAC
    ATATTTCAGT TGTCATCTCT GAATCCTCAA GAACGTATTG ATTATTGTCA TCTGATTGAA
    AAACTAGGTG GATCAGTGAT TGAGAAGCAG TGCTTTGATC CCAGCTGTAC ACACATTGTT
    GTGGGGCATC CACTTCGCAA CGAGAAGTAT TTAGCCTCCG TGGCAGCGGG AAAGTGGGTA
    CTCCATCGCT CCTACCTCGA AGCTTGCAGG ACTGCTGGGC GCTTCATGTC GGAAGAAGAC
    TATGAGTGGG GAAGTAGTTC CATACTTGAT GTTTTGACTG GAATCAATGT ACAGCAACGA
    AAACTAGCAC TTGCAGCAAT GAGATGGAGG AAAAAAATCC AGCAAAGACA AGAATCAGGC
    ATTACTGAGG GGGCATTTAG CGGGTGGAAA GTTATTTTAC ATGTTGATCA GTCCCGAGAA
    TCAGGATTCA AACGCCTACT TCAGTCAGGA GGAGCAAAGG TGCTGCCTGG TTATTCTGTA
    TCTTTATTTA AAGAAGCTAC ACATCTCTTT TCTGACTTTA ATAAACAGAA ACCAGATGAC
    TCAGGAGTTA ATATAGCAGA AGCTGCTGCC CAGAATGTAT ACTGCTTGAA AACAGAATAC
    ATTGCTGATT TTCTTATGCA GGAATCCCCT CCTCAAGTAG AAAATTACTG TCTACCAGAA
    GCTGCTTCAT TTCTTCAGAA TAAAAAGGAA CCTGGGCCTG GATTCTCACA AAAGAGGAAA
    GCTCCTGCAG AAAAAAATAA AATCAAACGA CCCAGAGTAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202018.1
    CDS311..4615
    Translation

    Target ORF information:

    RefSeq Version XM_005201961.3
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201961.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCACCACC AGCGATGTGT CCCAAGAGCT GAACATCCAG TTTATAACAT GGTTATGTCT 
    GATGTAACCG TGTCCTGTAC AAGCCTGGAA AAAGACAGAA GGGAAGAAGT TCATAAATAT
    GTACAAATGA TGGGTGGACG CGTATACAGA GACCTTAACG TATCAGTAAC TCACCTTATT
    GCAGGAGAAG TTGGTAGCAA AAAATACTTA GTTGCTGCAA ACCTAAAGAA ACCTATTTTG
    CTTCCCTCTT GGATAAAAAC ACTTTGGGAG AAGTCACAGG AGAAGAAAAT AGCTAGATAT
    ACTGATATAA ACATGGAAGA CTTCAAGTGT CCTATTTTTC TTGGTTGCAT AATCTGTGTG
    ACTGGCTTAT GTACCTTAGA CAGGAAATCA GTTCAGCAGC TAACAGTAAA GCATGGAGGA
    CAGTACATGG GACAGTTGAA AATGAATGAG TGTACACACC TCATTGTGCA AGAACCAAAA
    GGTCAAAAAT ATGAATGTGC CAAGAGATGG AATGTACACT GTGTGACCAC GCAGTGGTTT
    TTTGACAGTG TTGAGAAAGG TTTTTGTCAG GATGAATCCA TATACAAGAC AGAGCCTAGA
    TCAGAAGCAA AGAGTATGCC TGATACTTCA ACTCCTACTG GCCAGATCAA CACAATTGAT
    AGTCGTACTC TTTCAGATGT CAGCCATATT TCCAACATCA ATGCAAGCTG CATAAATGAA
    TCAATATGTA ATTCAGTTAA TAGCAAACTG GAACCTACAA TTGAAAATCT GGAAAATTTG
    GATATCAGTG CATTTCAGGC ACCTGAAGAT TTATTAGATG GTTGTCGGAT CTATCTTTGT
    GGTTTTAATG GCAGAAAGCT AGATAAACTG AGAAGACTTA TTAACAGTGG AGGTGGAGTT
    CGTTTTAATC AGCTCAATGA AGATGTAACT CATGTTATTG TGGGAGATGA TGATGATGAA
    TTGAAGCAGT TTTGGGATAA ATCAGCCCAC AGGCCGCATG TAGTGGGAGC AAAATGGTTG
    CTAGAGTGTT TTAGTAAAGG CTACATGCTT CCTGAAGAGC CATACATCCA CGTTAACTAC
    CAGCCAGTGG AAATTCCAGT TTCAGATCCA CCTGAAAGTA AAACAGCCCT TTTGAAAAGG
    AAGAACAACG GCTTCTCTAA GAGTGACTTT GTTCCTGAGG AGAAGCATGA GCAAGCTGAT
    GAAGATCTGC TCTCTCAGTA TCAGACTAAT AACCCCACAG TAGTTGAAGT TGCCAAGTCT
    GAAGTTGGGC TCTGTAGTGA CTCTACTCAT GTGGAGCCCC GTAATGATTC TACTCATATT
    TCTCTGCAAG AACACCAGTC TTCCATCAGT CATTGCCTCC CTGATGTTTC TACAATTACC
    GAAGAAGGTT TATTTAGCCA AAAGAGTTTC CTAGTTTTGG GGTTTAGTAA TGAAAATGAA
    TCTAATATTG CAATCATCAT AAGAGAAAAT GCTGGAAAAA TTGTGTCCCT TCAGAGCAGA
    GTTGTTGCTG ATTATGCTGT GGTTCCTCTG CTGGGATGTG AAGTACAGGC AACTGTGGGA
    GAAGTTGTAA CAAACACATG GCTGGTAATG TGTATAGACT ACCAGACTTT AATTGATCCA
    AAGTCAAATC CTCTCTTCAC ACCAGTTCCA GTTATGGCAG GAATGACTCC TTTAGAGGGT
    TGTGTTCTTT CATTCAGCCA ATGTGTAGGA GCTGAAAAAG ATTCATTGAC ATTCTTAGCA
    AATCACCTTG GAGCCAGTGT TCAAGAATAC TTCGTTCGCA AGTCCAATGC AAAGAAAGGC
    ATGTTTGCTA GTACACATCT TGTTTTGAAA GAGCCAGGTG GTTCTAAATA TGAAGCTGCC
    AAGAAGTGGA ATTTACCAGC AGTCACCATA GCTTGGCTGT TAGAGTCTGC TAGATTGGGA
    AAGAGAGCAA ATGAAAACCA TTTCCTGATT GAAAATTCAT CTAAAGAAGA ACAAAGTTTG
    GAAACTGAAA CAACAAATGA GGTGAATCTA AATCCAGATA CTCCAGAGCA TCCTGTAACA
    CATCTGGAAA CTCAGAGAAA AACAGCCGTT ACACCTTTAG ATATGAACCG CTTTCAGAGT
    AAAGCTTTCC GCACGGTGAT GTCACAGCAC TCCGGACAGG CTTCAGTGTC CCCTTCCCCA
    GGACAGTCAC TTCAGAAGGA GCCCTCATTA CACCTGGATA CGCCGTCAAA GTTTCTGTCC
    AAGGACAAAC TGTTCAAGCC TTCCTTTGAT GTGAAGGATG CTCTCGCAGC CTTGGAAACT
    CCAGGAGGTC CCAGCCAGCA GAAAAGGAAA CTGAGTACAC CACTCTCAGA AGTCATCGTC
    AAAAACCTGA AACTTGCTTT GGCAAATAGC TCTCGAAATG CTGTTGCTCT TTCTGCCAGC
    CCTCAACTGA AGGATGCCCA TTCAGAAAAG GAAGATGCTC CGAAGCCACT TGAGAACGTT
    GTGGTGTGTG TTAGTAAGAA ACTCAGTAAG AAGCAGAGTG AACTAAATGG GATTGCGGCC
    TCTCTAGGTG CAGACTACAG GTGGAGTTTT GATGAGACAG TGACTCATTT CATCTATCAA
    GGACGACCAA ATGACACTAA TCGGGAGTAT AAATCTGTAA AAGAAAGAGG AGTACACATT
    GTTTCAGAGC ACTGGCTTTT AGAGTGTGCC CAGGAGTATA AACATCTTCC CGAATCTCTT
    TATCCACATA CTTACAATCC CAAAATGAGC TTGGACATCA GTGCAGTGCA AGATGGCCGA
    CTCTGTAATA ACCGACTACT TTCAGCTGGC TCTGCAATGA GGGATGATGA GCCAGATCAT
    ATGCCTCTAG AAGAAAATGA TATAGACAAT ATGACCACCA GTAATAAAGA ACTAACAACA
    TCAAATGGAA ATGGGAGGAA TGACTCTAAA GGAGCTCTGA CACAGACTTT GGAGATGAGA
    GAGAACTTTC AAAAGCAGCT TCAGGAGATA ATGTCTGCAA CTTCAATAGT GAAACCCCAA
    GGGCAGAGGA CTTCCCTCTC AAGAAGTGGT TGTAACAGTA CCTCTTCAAC TCCTGACAGC
    ACCCGCTCTG CCCGCAGTGG ACGAAGTAGA GTCCTAGAGG CACTGAGGCA GTCTCGTCAA
    ACAGTACCTG ATGTCAACAC AGAGCCCTCC CAAAATGAAC AGATCATTTG GGATGACCCT
    ACAGCCAGGG AGGAGAGAGC AAGACTTGCC AGCAATTTGC AGTGGCCTAG TTGTCCCACA
    CAATACTCTG AGCTTCAGGT TGATGTTAAA AAACTGGAGG ATTCTCCTTT CCAGGAGCCT
    TTACATGATT CAGAAATTGC TGAACAGGCT GTCTGTGATC CTGGGAACGT ATGTGTGACC
    GGAGCCCCAA AACACCCGAT CTCTGAAGAA CTGGAAGCTC CAGTGAAAGA CAGCCACCTG
    ATCCCTACGC CTCAAGCCCC CAGTATTGCC TTCCCCCTAG CCAACCCACC TGTGGCTCCA
    CACCCTAGAG AAAAGATTAT AACAATAGAG GAGACTCATG AAGACTTAAA AAAACAGTAC
    ATATTTCAGT TGTCATCTCT GAATCCTCAA GAACGTATTG ATTATTGTCA TCTGATTGAA
    AAACTAGGTG GATCAGTGAT TGAGAAGCAG TGCTTTGATC CCAGCTGTAC ACACATTGTT
    GTGGGGCATC CACTTCGCAA CGAGAAGTAT TTAGCCTCCG TGGCAGCGGG AAAGTGGGTA
    CTCCATCGCT CCTACCTCGA AGCTTGCAGG ACTGCTGGGC GCTTCATGTC GGAAGAAGAC
    TATGAGTGGG GAAGTAGTTC CATACTTGAT GTTTTGACTG GAATCAATGT ACAGCAACGA
    AAACTAGCAC TTGCAGCAAT GAGATGGAGG AAAAAAATCC AGCAAAGACA AGAATCAGGC
    ATTACTGAGG GGGCATTTAG CGGGTGGAAA GTTATTTTAC ATGTTGATCA GTCCCGAGAA
    TCAGGATTCA AACGCCTACT TCAGTCAGGA GGAGCAAAGG TGCTGCCTGG TTATTCTGTA
    TCTTTATTTA AAGAAGCTAC ACATCTCTTT TCTGACTTTA ATAAACAGAA ACCAGATGAC
    TCAGGAGTTA ATATAGCAGA AGCTGCTGCC CAGAATGTAT ACTGCTTGAA AACAGAATAC
    ATTGCTGATT TTCTTATGCA GGAATCCCCT CCTCAAGTAG AAAATTACTG TCTACCAGAA
    GCTGCTTCAT TTCTTCAGAA TAAAAAGGAA CCTGGGCCTG GATTCTCACA AAAGAGGAAA
    GCTCCTGCAG AAAAAAATAA AATCAAACGA CCCAGAGTAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16053
    Clone ID Related Accession (Same CDS sequence) XM_005201961.3 , XM_024991344.1
    Accession Version XM_024991344.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-binding protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCACCACC AGCGATGTGT CCCAAGAGCT GAACATCCAG TTTATAACAT GGTTATGTCT 
    GATGTAACCG TGTCCTGTAC AAGCCTGGAA AAAGACAGAA GGGAAGAAGT TCATAAATAT
    GTACAAATGA TGGGTGGACG CGTATACAGA GACCTTAACG TATCAGTAAC TCACCTTATT
    GCAGGAGAAG TTGGTAGCAA AAAATACTTA GTTGCTGCAA ACCTAAAGAA ACCTATTTTG
    CTTCCCTCTT GGATAAAAAC ACTTTGGGAG AAGTCACAGG AGAAGAAAAT AGCTAGATAT
    ACTGATATAA ACATGGAAGA CTTCAAGTGT CCTATTTTTC TTGGTTGCAT AATCTGTGTG
    ACTGGCTTAT GTACCTTAGA CAGGAAATCA GTTCAGCAGC TAACAGTAAA GCATGGAGGA
    CAGTACATGG GACAGTTGAA AATGAATGAG TGTACACACC TCATTGTGCA AGAACCAAAA
    GGTCAAAAAT ATGAATGTGC CAAGAGATGG AATGTACACT GTGTGACCAC GCAGTGGTTT
    TTTGACAGTG TTGAGAAAGG TTTTTGTCAG GATGAATCCA TATACAAGAC AGAGCCTAGA
    TCAGAAGCAA AGAGTATGCC TGATACTTCA ACTCCTACTG GCCAGATCAA CACAATTGAT
    AGTCGTACTC TTTCAGATGT CAGCCATATT TCCAACATCA ATGCAAGCTG CATAAATGAA
    TCAATATGTA ATTCAGTTAA TAGCAAACTG GAACCTACAA TTGAAAATCT GGAAAATTTG
    GATATCAGTG CATTTCAGGC ACCTGAAGAT TTATTAGATG GTTGTCGGAT CTATCTTTGT
    GGTTTTAATG GCAGAAAGCT AGATAAACTG AGAAGACTTA TTAACAGTGG AGGTGGAGTT
    CGTTTTAATC AGCTCAATGA AGATGTAACT CATGTTATTG TGGGAGATGA TGATGATGAA
    TTGAAGCAGT TTTGGGATAA ATCAGCCCAC AGGCCGCATG TAGTGGGAGC AAAATGGTTG
    CTAGAGTGTT TTAGTAAAGG CTACATGCTT CCTGAAGAGC CATACATCCA CGTTAACTAC
    CAGCCAGTGG AAATTCCAGT TTCAGATCCA CCTGAAAGTA AAACAGCCCT TTTGAAAAGG
    AAGAACAACG GCTTCTCTAA GAGTGACTTT GTTCCTGAGG AGAAGCATGA GCAAGCTGAT
    GAAGATCTGC TCTCTCAGTA TCAGACTAAT AACCCCACAG TAGTTGAAGT TGCCAAGTCT
    GAAGTTGGGC TCTGTAGTGA CTCTACTCAT GTGGAGCCCC GTAATGATTC TACTCATATT
    TCTCTGCAAG AACACCAGTC TTCCATCAGT CATTGCCTCC CTGATGTTTC TACAATTACC
    GAAGAAGGTT TATTTAGCCA AAAGAGTTTC CTAGTTTTGG GGTTTAGTAA TGAAAATGAA
    TCTAATATTG CAATCATCAT AAGAGAAAAT GCTGGAAAAA TTGTGTCCCT TCAGAGCAGA
    GTTGTTGCTG ATTATGCTGT GGTTCCTCTG CTGGGATGTG AAGTACAGGC AACTGTGGGA
    GAAGTTGTAA CAAACACATG GCTGGTAATG TGTATAGACT ACCAGACTTT AATTGATCCA
    AAGTCAAATC CTCTCTTCAC ACCAGTTCCA GTTATGGCAG GAATGACTCC TTTAGAGGGT
    TGTGTTCTTT CATTCAGCCA ATGTGTAGGA GCTGAAAAAG ATTCATTGAC ATTCTTAGCA
    AATCACCTTG GAGCCAGTGT TCAAGAATAC TTCGTTCGCA AGTCCAATGC AAAGAAAGGC
    ATGTTTGCTA GTACACATCT TGTTTTGAAA GAGCCAGGTG GTTCTAAATA TGAAGCTGCC
    AAGAAGTGGA ATTTACCAGC AGTCACCATA GCTTGGCTGT TAGAGTCTGC TAGATTGGGA
    AAGAGAGCAA ATGAAAACCA TTTCCTGATT GAAAATTCAT CTAAAGAAGA ACAAAGTTTG
    GAAACTGAAA CAACAAATGA GGTGAATCTA AATCCAGATA CTCCAGAGCA TCCTGTAACA
    CATCTGGAAA CTCAGAGAAA AACAGCCGTT ACACCTTTAG ATATGAACCG CTTTCAGAGT
    AAAGCTTTCC GCACGGTGAT GTCACAGCAC TCCGGACAGG CTTCAGTGTC CCCTTCCCCA
    GGACAGTCAC TTCAGAAGGA GCCCTCATTA CACCTGGATA CGCCGTCAAA GTTTCTGTCC
    AAGGACAAAC TGTTCAAGCC TTCCTTTGAT GTGAAGGATG CTCTCGCAGC CTTGGAAACT
    CCAGGAGGTC CCAGCCAGCA GAAAAGGAAA CTGAGTACAC CACTCTCAGA AGTCATCGTC
    AAAAACCTGA AACTTGCTTT GGCAAATAGC TCTCGAAATG CTGTTGCTCT TTCTGCCAGC
    CCTCAACTGA AGGATGCCCA TTCAGAAAAG GAAGATGCTC CGAAGCCACT TGAGAACGTT
    GTGGTGTGTG TTAGTAAGAA ACTCAGTAAG AAGCAGAGTG AACTAAATGG GATTGCGGCC
    TCTCTAGGTG CAGACTACAG GTGGAGTTTT GATGAGACAG TGACTCATTT CATCTATCAA
    GGACGACCAA ATGACACTAA TCGGGAGTAT AAATCTGTAA AAGAAAGAGG AGTACACATT
    GTTTCAGAGC ACTGGCTTTT AGAGTGTGCC CAGGAGTATA AACATCTTCC CGAATCTCTT
    TATCCACATA CTTACAATCC CAAAATGAGC TTGGACATCA GTGCAGTGCA AGATGGCCGA
    CTCTGTAATA ACCGACTACT TTCAGCTGGC TCTGCAATGA GGGATGATGA GCCAGATCAT
    ATGCCTCTAG AAGAAAATGA TATAGACAAT ATGACCACCA GTAATAAAGA ACTAACAACA
    TCAAATGGAA ATGGGAGGAA TGACTCTAAA GGAGCTCTGA CACAGACTTT GGAGATGAGA
    GAGAACTTTC AAAAGCAGCT TCAGGAGATA ATGTCTGCAA CTTCAATAGT GAAACCCCAA
    GGGCAGAGGA CTTCCCTCTC AAGAAGTGGT TGTAACAGTA CCTCTTCAAC TCCTGACAGC
    ACCCGCTCTG CCCGCAGTGG ACGAAGTAGA GTCCTAGAGG CACTGAGGCA GTCTCGTCAA
    ACAGTACCTG ATGTCAACAC AGAGCCCTCC CAAAATGAAC AGATCATTTG GGATGACCCT
    ACAGCCAGGG AGGAGAGAGC AAGACTTGCC AGCAATTTGC AGTGGCCTAG TTGTCCCACA
    CAATACTCTG AGCTTCAGGT TGATGTTAAA AAACTGGAGG ATTCTCCTTT CCAGGAGCCT
    TTACATGATT CAGAAATTGC TGAACAGGCT GTCTGTGATC CTGGGAACGT ATGTGTGACC
    GGAGCCCCAA AACACCCGAT CTCTGAAGAA CTGGAAGCTC CAGTGAAAGA CAGCCACCTG
    ATCCCTACGC CTCAAGCCCC CAGTATTGCC TTCCCCCTAG CCAACCCACC TGTGGCTCCA
    CACCCTAGAG AAAAGATTAT AACAATAGAG GAGACTCATG AAGACTTAAA AAAACAGTAC
    ATATTTCAGT TGTCATCTCT GAATCCTCAA GAACGTATTG ATTATTGTCA TCTGATTGAA
    AAACTAGGTG GATCAGTGAT TGAGAAGCAG TGCTTTGATC CCAGCTGTAC ACACATTGTT
    GTGGGGCATC CACTTCGCAA CGAGAAGTAT TTAGCCTCCG TGGCAGCGGG AAAGTGGGTA
    CTCCATCGCT CCTACCTCGA AGCTTGCAGG ACTGCTGGGC GCTTCATGTC GGAAGAAGAC
    TATGAGTGGG GAAGTAGTTC CATACTTGAT GTTTTGACTG GAATCAATGT ACAGCAACGA
    AAACTAGCAC TTGCAGCAAT GAGATGGAGG AAAAAAATCC AGCAAAGACA AGAATCAGGC
    ATTACTGAGG GGGCATTTAG CGGGTGGAAA GTTATTTTAC ATGTTGATCA GTCCCGAGAA
    TCAGGATTCA AACGCCTACT TCAGTCAGGA GGAGCAAAGG TGCTGCCTGG TTATTCTGTA
    TCTTTATTTA AAGAAGCTAC ACATCTCTTT TCTGACTTTA ATAAACAGAA ACCAGATGAC
    TCAGGAGTTA ATATAGCAGA AGCTGCTGCC CAGAATGTAT ACTGCTTGAA AACAGAATAC
    ATTGCTGATT TTCTTATGCA GGAATCCCCT CCTCAAGTAG AAAATTACTG TCTACCAGAA
    GCTGCTTCAT TTCTTCAGAA TAAAAAGGAA CCTGGGCCTG GATTCTCACA AAAGAGGAAA
    GCTCCTGCAG AAAAAAATAA AATCAAACGA CCCAGAGTAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847112.1
    CDS590..4894
    Translation

    Target ORF information:

    RefSeq Version XM_024991344.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991344.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCACCACC AGCGATGTGT CCCAAGAGCT GAACATCCAG TTTATAACAT GGTTATGTCT 
    GATGTAACCG TGTCCTGTAC AAGCCTGGAA AAAGACAGAA GGGAAGAAGT TCATAAATAT
    GTACAAATGA TGGGTGGACG CGTATACAGA GACCTTAACG TATCAGTAAC TCACCTTATT
    GCAGGAGAAG TTGGTAGCAA AAAATACTTA GTTGCTGCAA ACCTAAAGAA ACCTATTTTG
    CTTCCCTCTT GGATAAAAAC ACTTTGGGAG AAGTCACAGG AGAAGAAAAT AGCTAGATAT
    ACTGATATAA ACATGGAAGA CTTCAAGTGT CCTATTTTTC TTGGTTGCAT AATCTGTGTG
    ACTGGCTTAT GTACCTTAGA CAGGAAATCA GTTCAGCAGC TAACAGTAAA GCATGGAGGA
    CAGTACATGG GACAGTTGAA AATGAATGAG TGTACACACC TCATTGTGCA AGAACCAAAA
    GGTCAAAAAT ATGAATGTGC CAAGAGATGG AATGTACACT GTGTGACCAC GCAGTGGTTT
    TTTGACAGTG TTGAGAAAGG TTTTTGTCAG GATGAATCCA TATACAAGAC AGAGCCTAGA
    TCAGAAGCAA AGAGTATGCC TGATACTTCA ACTCCTACTG GCCAGATCAA CACAATTGAT
    AGTCGTACTC TTTCAGATGT CAGCCATATT TCCAACATCA ATGCAAGCTG CATAAATGAA
    TCAATATGTA ATTCAGTTAA TAGCAAACTG GAACCTACAA TTGAAAATCT GGAAAATTTG
    GATATCAGTG CATTTCAGGC ACCTGAAGAT TTATTAGATG GTTGTCGGAT CTATCTTTGT
    GGTTTTAATG GCAGAAAGCT AGATAAACTG AGAAGACTTA TTAACAGTGG AGGTGGAGTT
    CGTTTTAATC AGCTCAATGA AGATGTAACT CATGTTATTG TGGGAGATGA TGATGATGAA
    TTGAAGCAGT TTTGGGATAA ATCAGCCCAC AGGCCGCATG TAGTGGGAGC AAAATGGTTG
    CTAGAGTGTT TTAGTAAAGG CTACATGCTT CCTGAAGAGC CATACATCCA CGTTAACTAC
    CAGCCAGTGG AAATTCCAGT TTCAGATCCA CCTGAAAGTA AAACAGCCCT TTTGAAAAGG
    AAGAACAACG GCTTCTCTAA GAGTGACTTT GTTCCTGAGG AGAAGCATGA GCAAGCTGAT
    GAAGATCTGC TCTCTCAGTA TCAGACTAAT AACCCCACAG TAGTTGAAGT TGCCAAGTCT
    GAAGTTGGGC TCTGTAGTGA CTCTACTCAT GTGGAGCCCC GTAATGATTC TACTCATATT
    TCTCTGCAAG AACACCAGTC TTCCATCAGT CATTGCCTCC CTGATGTTTC TACAATTACC
    GAAGAAGGTT TATTTAGCCA AAAGAGTTTC CTAGTTTTGG GGTTTAGTAA TGAAAATGAA
    TCTAATATTG CAATCATCAT AAGAGAAAAT GCTGGAAAAA TTGTGTCCCT TCAGAGCAGA
    GTTGTTGCTG ATTATGCTGT GGTTCCTCTG CTGGGATGTG AAGTACAGGC AACTGTGGGA
    GAAGTTGTAA CAAACACATG GCTGGTAATG TGTATAGACT ACCAGACTTT AATTGATCCA
    AAGTCAAATC CTCTCTTCAC ACCAGTTCCA GTTATGGCAG GAATGACTCC TTTAGAGGGT
    TGTGTTCTTT CATTCAGCCA ATGTGTAGGA GCTGAAAAAG ATTCATTGAC ATTCTTAGCA
    AATCACCTTG GAGCCAGTGT TCAAGAATAC TTCGTTCGCA AGTCCAATGC AAAGAAAGGC
    ATGTTTGCTA GTACACATCT TGTTTTGAAA GAGCCAGGTG GTTCTAAATA TGAAGCTGCC
    AAGAAGTGGA ATTTACCAGC AGTCACCATA GCTTGGCTGT TAGAGTCTGC TAGATTGGGA
    AAGAGAGCAA ATGAAAACCA TTTCCTGATT GAAAATTCAT CTAAAGAAGA ACAAAGTTTG
    GAAACTGAAA CAACAAATGA GGTGAATCTA AATCCAGATA CTCCAGAGCA TCCTGTAACA
    CATCTGGAAA CTCAGAGAAA AACAGCCGTT ACACCTTTAG ATATGAACCG CTTTCAGAGT
    AAAGCTTTCC GCACGGTGAT GTCACAGCAC TCCGGACAGG CTTCAGTGTC CCCTTCCCCA
    GGACAGTCAC TTCAGAAGGA GCCCTCATTA CACCTGGATA CGCCGTCAAA GTTTCTGTCC
    AAGGACAAAC TGTTCAAGCC TTCCTTTGAT GTGAAGGATG CTCTCGCAGC CTTGGAAACT
    CCAGGAGGTC CCAGCCAGCA GAAAAGGAAA CTGAGTACAC CACTCTCAGA AGTCATCGTC
    AAAAACCTGA AACTTGCTTT GGCAAATAGC TCTCGAAATG CTGTTGCTCT TTCTGCCAGC
    CCTCAACTGA AGGATGCCCA TTCAGAAAAG GAAGATGCTC CGAAGCCACT TGAGAACGTT
    GTGGTGTGTG TTAGTAAGAA ACTCAGTAAG AAGCAGAGTG AACTAAATGG GATTGCGGCC
    TCTCTAGGTG CAGACTACAG GTGGAGTTTT GATGAGACAG TGACTCATTT CATCTATCAA
    GGACGACCAA ATGACACTAA TCGGGAGTAT AAATCTGTAA AAGAAAGAGG AGTACACATT
    GTTTCAGAGC ACTGGCTTTT AGAGTGTGCC CAGGAGTATA AACATCTTCC CGAATCTCTT
    TATCCACATA CTTACAATCC CAAAATGAGC TTGGACATCA GTGCAGTGCA AGATGGCCGA
    CTCTGTAATA ACCGACTACT TTCAGCTGGC TCTGCAATGA GGGATGATGA GCCAGATCAT
    ATGCCTCTAG AAGAAAATGA TATAGACAAT ATGACCACCA GTAATAAAGA ACTAACAACA
    TCAAATGGAA ATGGGAGGAA TGACTCTAAA GGAGCTCTGA CACAGACTTT GGAGATGAGA
    GAGAACTTTC AAAAGCAGCT TCAGGAGATA ATGTCTGCAA CTTCAATAGT GAAACCCCAA
    GGGCAGAGGA CTTCCCTCTC AAGAAGTGGT TGTAACAGTA CCTCTTCAAC TCCTGACAGC
    ACCCGCTCTG CCCGCAGTGG ACGAAGTAGA GTCCTAGAGG CACTGAGGCA GTCTCGTCAA
    ACAGTACCTG ATGTCAACAC AGAGCCCTCC CAAAATGAAC AGATCATTTG GGATGACCCT
    ACAGCCAGGG AGGAGAGAGC AAGACTTGCC AGCAATTTGC AGTGGCCTAG TTGTCCCACA
    CAATACTCTG AGCTTCAGGT TGATGTTAAA AAACTGGAGG ATTCTCCTTT CCAGGAGCCT
    TTACATGATT CAGAAATTGC TGAACAGGCT GTCTGTGATC CTGGGAACGT ATGTGTGACC
    GGAGCCCCAA AACACCCGAT CTCTGAAGAA CTGGAAGCTC CAGTGAAAGA CAGCCACCTG
    ATCCCTACGC CTCAAGCCCC CAGTATTGCC TTCCCCCTAG CCAACCCACC TGTGGCTCCA
    CACCCTAGAG AAAAGATTAT AACAATAGAG GAGACTCATG AAGACTTAAA AAAACAGTAC
    ATATTTCAGT TGTCATCTCT GAATCCTCAA GAACGTATTG ATTATTGTCA TCTGATTGAA
    AAACTAGGTG GATCAGTGAT TGAGAAGCAG TGCTTTGATC CCAGCTGTAC ACACATTGTT
    GTGGGGCATC CACTTCGCAA CGAGAAGTAT TTAGCCTCCG TGGCAGCGGG AAAGTGGGTA
    CTCCATCGCT CCTACCTCGA AGCTTGCAGG ACTGCTGGGC GCTTCATGTC GGAAGAAGAC
    TATGAGTGGG GAAGTAGTTC CATACTTGAT GTTTTGACTG GAATCAATGT ACAGCAACGA
    AAACTAGCAC TTGCAGCAAT GAGATGGAGG AAAAAAATCC AGCAAAGACA AGAATCAGGC
    ATTACTGAGG GGGCATTTAG CGGGTGGAAA GTTATTTTAC ATGTTGATCA GTCCCGAGAA
    TCAGGATTCA AACGCCTACT TCAGTCAGGA GGAGCAAAGG TGCTGCCTGG TTATTCTGTA
    TCTTTATTTA AAGAAGCTAC ACATCTCTTT TCTGACTTTA ATAAACAGAA ACCAGATGAC
    TCAGGAGTTA ATATAGCAGA AGCTGCTGCC CAGAATGTAT ACTGCTTGAA AACAGAATAC
    ATTGCTGATT TTCTTATGCA GGAATCCCCT CCTCAAGTAG AAAATTACTG TCTACCAGAA
    GCTGCTTCAT TTCTTCAGAA TAAAAAGGAA CCTGGGCCTG GATTCTCACA AAAGAGGAAA
    GCTCCTGCAG AAAAAAATAA AATCAAACGA CCCAGAGTAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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