MAP4K4 cDNA ORF clone, Canis lupus familiaris(dog)

The following MAP4K4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP4K4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe291541 XM_022424558.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe291542 XM_022424559.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe291543 XM_022424560.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe291544 XM_022424561.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe291545 XM_022424562.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe291546 XM_022424563.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe291547 XM_022424564.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe291548 XM_022424565.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe291549 XM_022424566.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe291550 XM_022424567.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe291551 XM_022424568.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe291552 XM_022424569.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe291553 XM_022424570.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe291554 XM_022424571.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe291555 XM_022424573.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe291556 XM_022424574.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe291557 XM_022424575.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe291558 XM_022424576.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe291559 XM_022424577.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe291560 XM_022424578.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe38955 XM_014117435.2
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
View Old Accession Versions >>
OCe38955 XM_014117435.1 Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe291541
    Clone ID Related Accession (Same CDS sequence) XM_022424558.1
    Accession Version XM_022424558.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGGTACAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCTCCCAAAT TTGCACACCA CCATCTTCGC
    TCTCAGGACC CATGTCCACC TTCCCGCAGT GAGGTGCTAA GTCAGAGTTC TGACTCTAAG
    TCGGAGGTAC CTGACCCCAC CCAAAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT
    CCAAGGGTTC CTGTGAGGAC AACGTCTCGT TCCCCTGTTC TGTCCCGTCG GGATTCCCCA
    CTGCAAGGCA GTGGACAGCA AAATAGTCAA GCAGGTCAAA GAAACTCCAC TAGCAGTATT
    GAGCCCCGGC TGCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC
    TCCTCCGGAT CCAGCAACTC CGGATCCCAG CCTGGGTCCC ACCCTGGGTC CCAGAGTGGC
    TCTGGAGAGC GCTTCAGAGT GAGATCATCA TCCAAATCTG AAGGCTCTCC TTCTCAGCGC
    CTAGAAAATG CAGTGAAAAA ACCTGAAGAA AAGAAAGAAG TTTTCAGACC TCTCAAGCCT
    GCCGGCGAAG TGGATTTGAC TGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTGCGA
    CCACCACACA AAGTGACAGA CTACTCCTCA TCCAGTGAGG AGTCCGGGAC AACAGATGAG
    GAAGACGATG ATGTAGAACA AGAAGGGGCT GAGGAAGCCA CCTCTGGACC AGAGGACACC
    AGAGCAGCGT CGTCCCTGAA TTTGAGCAAT GGTGAAACAG AATCCGTGAA AACCATGATT
    GTTCATGACG ACGTAGAAAG TGAACCAGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC
    GTCCGCCAGA GTACAGTTGA CCAAAAGCGC GCCAGCCATC ATGAGAGCAA TGGCTTTGCC
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC
    ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG
    GACAAGCTCA CTACTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGTGGG
    ACAACAGTGA CTTCTGTGGT GGGATTTTCC TGTGATGGAA TGAGACCAGA AGCCATAAGG
    CAAGATCCTA CCCGGAAGGG CTCAGTGGTC AATGTGAATC CCACCAACAC TAGGCCACAG
    AGTGATACCC CGGAGATTCG TAAATATAAG AAGAGATTTA ACTCCGAGAT TCTGTGTGCT
    GCCTTATGGG GAGTGAATTT GCTAGTGGGT ACAGAGAGTG GCCTGATGCT GCTGGACAGA
    AGTGGCCAAG GGAAGGTATA TCCCCTGATC AACCGAAGAC GATTTCAGCA AATGGATGTC
    CTTGAAGGCT TGAATGTCTT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC
    TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAATGATC CAGAAGTTGA GAAGAAGCAG
    GGATGGACGA CTGTGGGAGA TTTGGAAGGA TGTGTACACT ATAAAGTTGT AAAATATGAA
    AGAATCAAAT TTCTGGTAAT TGCTTTGAAG AGTTCTGTGG AGGTCTATGC GTGGGCACCC
    AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGGG AATTAGTACA TAAGCCATTG
    CTGGTGGATC TCACTGTGGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT
    GGATTCCATG CTGTTGATGT GGATTCTGGA TCAGTCTATG ACATTTATCT ACCAACACAC
    GTAAGAAAGA ACCCACACTC TATGATCCAG TGTAGCATCA AACCCCATGC AATCATCATC
    CTCCCCAACA CAGATGGCAT GGAGCTTCTG GTGTGCTATG AAGATGAAGG GGTTTATGTG
    AATACTTATG GAAGAATCAC CAAGGATGTG GTTCTGCAGT GGGGAGAGAT GCCAACATCT
    GTAGCATATA TTCGATCCAA CCAGACGATG GGCTGGGGAG AAAAGGCCAT AGAGATCCGA
    TCTGTGGAAA CTGGTCATTT GGATGGTGTG TTTATGCACA AAAGGGCTCA AAGACTAAAA
    TTCCTATGTG AACGCAATGA CAAGGTCTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGC
    CAGGTTTATT TCATGACGTT AGGCAGGACT TCTCTTCTGA GCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280266.1
    CDS175..4182
    Translation

    Target ORF information:

    RefSeq Version XM_022424558.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424558.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGGTACAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCTCCCAAAT TTGCACACCA CCATCTTCGC
    TCTCAGGACC CATGTCCACC TTCCCGCAGT GAGGTGCTAA GTCAGAGTTC TGACTCTAAG
    TCGGAGGTAC CTGACCCCAC CCAAAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT
    CCAAGGGTTC CTGTGAGGAC AACGTCTCGT TCCCCTGTTC TGTCCCGTCG GGATTCCCCA
    CTGCAAGGCA GTGGACAGCA AAATAGTCAA GCAGGTCAAA GAAACTCCAC TAGCAGTATT
    GAGCCCCGGC TGCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC
    TCCTCCGGAT CCAGCAACTC CGGATCCCAG CCTGGGTCCC ACCCTGGGTC CCAGAGTGGC
    TCTGGAGAGC GCTTCAGAGT GAGATCATCA TCCAAATCTG AAGGCTCTCC TTCTCAGCGC
    CTAGAAAATG CAGTGAAAAA ACCTGAAGAA AAGAAAGAAG TTTTCAGACC TCTCAAGCCT
    GCCGGCGAAG TGGATTTGAC TGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTGCGA
    CCACCACACA AAGTGACAGA CTACTCCTCA TCCAGTGAGG AGTCCGGGAC AACAGATGAG
    GAAGACGATG ATGTAGAACA AGAAGGGGCT GAGGAAGCCA CCTCTGGACC AGAGGACACC
    AGAGCAGCGT CGTCCCTGAA TTTGAGCAAT GGTGAAACAG AATCCGTGAA AACCATGATT
    GTTCATGACG ACGTAGAAAG TGAACCAGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC
    GTCCGCCAGA GTACAGTTGA CCAAAAGCGC GCCAGCCATC ATGAGAGCAA TGGCTTTGCC
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC
    ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG
    GACAAGCTCA CTACTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGTGGG
    ACAACAGTGA CTTCTGTGGT GGGATTTTCC TGTGATGGAA TGAGACCAGA AGCCATAAGG
    CAAGATCCTA CCCGGAAGGG CTCAGTGGTC AATGTGAATC CCACCAACAC TAGGCCACAG
    AGTGATACCC CGGAGATTCG TAAATATAAG AAGAGATTTA ACTCCGAGAT TCTGTGTGCT
    GCCTTATGGG GAGTGAATTT GCTAGTGGGT ACAGAGAGTG GCCTGATGCT GCTGGACAGA
    AGTGGCCAAG GGAAGGTATA TCCCCTGATC AACCGAAGAC GATTTCAGCA AATGGATGTC
    CTTGAAGGCT TGAATGTCTT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC
    TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAATGATC CAGAAGTTGA GAAGAAGCAG
    GGATGGACGA CTGTGGGAGA TTTGGAAGGA TGTGTACACT ATAAAGTTGT AAAATATGAA
    AGAATCAAAT TTCTGGTAAT TGCTTTGAAG AGTTCTGTGG AGGTCTATGC GTGGGCACCC
    AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGGG AATTAGTACA TAAGCCATTG
    CTGGTGGATC TCACTGTGGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT
    GGATTCCATG CTGTTGATGT GGATTCTGGA TCAGTCTATG ACATTTATCT ACCAACACAC
    GTAAGAAAGA ACCCACACTC TATGATCCAG TGTAGCATCA AACCCCATGC AATCATCATC
    CTCCCCAACA CAGATGGCAT GGAGCTTCTG GTGTGCTATG AAGATGAAGG GGTTTATGTG
    AATACTTATG GAAGAATCAC CAAGGATGTG GTTCTGCAGT GGGGAGAGAT GCCAACATCT
    GTAGCATATA TTCGATCCAA CCAGACGATG GGCTGGGGAG AAAAGGCCAT AGAGATCCGA
    TCTGTGGAAA CTGGTCATTT GGATGGTGTG TTTATGCACA AAAGGGCTCA AAGACTAAAA
    TTCCTATGTG AACGCAATGA CAAGGTCTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGC
    CAGGTTTATT TCATGACGTT AGGCAGGACT TCTCTTCTGA GCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291542
    Clone ID Related Accession (Same CDS sequence) XM_022424559.1
    Accession Version XM_022424559.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGGTACAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCTCCCAAAT TTGCACACCA CCATCTTCGC
    TCTCAGGACC CATGTCCACC TTCCCGCAGT GAGGTGCTAA GTCAGAGTTC TGACTCTAAG
    TCGGAGGTAC CTGACCCCAC CCAAAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT
    CCAAGGGTTC CTGTGAGGAC AACGTCTCGT TCCCCTGTTC TGTCCCGTCG GGATTCCCCA
    CTGCAAGGCA GTGGACAGCA AAATAGTCAA GCAGGTCAAA GAAACTCCAC TAGTATTGAG
    CCCCGGCTGC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GGCCTGGCAG TGGCAGCTCC
    TCCGGATCCA GCAACTCCGG ATCCCAGCCT GGGTCCCACC CTGGGTCCCA GAGTGGCTCT
    GGAGAGCGCT TCAGAGTGAG ATCATCATCC AAATCTGAAG GCTCTCCTTC TCAGCGCCTA
    GAAAATGCAG TGAAAAAACC TGAAGAAAAG AAAGAAGTTT TCAGACCTCT CAAGCCTGCC
    GGCGAAGTGG ATTTGACTGC ACTGGCCAAA GAGCTTCGAG CAGTGGAAGA TGTGCGACCA
    CCACACAAAG TGACAGACTA CTCCTCATCC AGTGAGGAGT CCGGGACAAC AGATGAGGAA
    GACGATGATG TAGAACAAGA AGGGGCTGAG GAAGCCACCT CTGGACCAGA GGACACCAGA
    GCAGCGTCGT CCCTGAATTT GAGCAATGGT GAAACAGAAT CCGTGAAAAC CATGATTGTT
    CATGACGACG TAGAAAGTGA ACCAGCCATG ACCCCATCCA AGGAGGGCAC TCTAATCGTC
    CGCCAGAGTA CAGTTGACCA AAAGCGCGCC AGCCATCATG AGAGCAATGG CTTTGCCGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCCTCCTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTCACTA CTAATGAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGTGGGACA
    ACAGTGACTT CTGTGGTGGG ATTTTCCTGT GATGGAATGA GACCAGAAGC CATAAGGCAA
    GATCCTACCC GGAAGGGCTC AGTGGTCAAT GTGAATCCCA CCAACACTAG GCCACAGAGT
    GATACCCCGG AGATTCGTAA ATATAAGAAG AGATTTAACT CCGAGATTCT GTGTGCTGCC
    TTATGGGGAG TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT
    GGCCAAGGGA AGGTATATCC CCTGATCAAC CGAAGACGAT TTCAGCAAAT GGATGTCCTT
    GAAGGCTTGA ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT
    TTGTCCTGGT TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA
    TGGACGACTG TGGGAGATTT GGAAGGATGT GTACACTATA AAGTTGTAAA ATATGAAAGA
    ATCAAATTTC TGGTAATTGC TTTGAAGAGT TCTGTGGAGG TCTATGCGTG GGCACCCAAG
    CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGGGAAT TAGTACATAA GCCATTGCTG
    GTGGATCTCA CTGTGGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA
    TTCCATGCTG TTGATGTGGA TTCTGGATCA GTCTATGACA TTTATCTACC AACACACGTA
    AGAAAGAACC CACACTCTAT GATCCAGTGT AGCATCAAAC CCCATGCAAT CATCATCCTC
    CCCAACACAG ATGGCATGGA GCTTCTGGTG TGCTATGAAG ATGAAGGGGT TTATGTGAAT
    ACTTATGGAA GAATCACCAA GGATGTGGTT CTGCAGTGGG GAGAGATGCC AACATCTGTA
    GCATATATTC GATCCAACCA GACGATGGGC TGGGGAGAAA AGGCCATAGA GATCCGATCT
    GTGGAAACTG GTCATTTGGA TGGTGTGTTT ATGCACAAAA GGGCTCAAAG ACTAAAATTC
    CTATGTGAAC GCAATGACAA GGTCTTCTTT GCCTCTGTTC GGTCTGGTGG CAGCAGCCAG
    GTTTATTTCA TGACGTTAGG CAGGACTTCT CTTCTGAGCT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280267.1
    CDS175..4179
    Translation

    Target ORF information:

    RefSeq Version XM_022424559.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424559.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGGTACAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCTCCCAAAT TTGCACACCA CCATCTTCGC
    TCTCAGGACC CATGTCCACC TTCCCGCAGT GAGGTGCTAA GTCAGAGTTC TGACTCTAAG
    TCGGAGGTAC CTGACCCCAC CCAAAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT
    CCAAGGGTTC CTGTGAGGAC AACGTCTCGT TCCCCTGTTC TGTCCCGTCG GGATTCCCCA
    CTGCAAGGCA GTGGACAGCA AAATAGTCAA GCAGGTCAAA GAAACTCCAC TAGTATTGAG
    CCCCGGCTGC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GGCCTGGCAG TGGCAGCTCC
    TCCGGATCCA GCAACTCCGG ATCCCAGCCT GGGTCCCACC CTGGGTCCCA GAGTGGCTCT
    GGAGAGCGCT TCAGAGTGAG ATCATCATCC AAATCTGAAG GCTCTCCTTC TCAGCGCCTA
    GAAAATGCAG TGAAAAAACC TGAAGAAAAG AAAGAAGTTT TCAGACCTCT CAAGCCTGCC
    GGCGAAGTGG ATTTGACTGC ACTGGCCAAA GAGCTTCGAG CAGTGGAAGA TGTGCGACCA
    CCACACAAAG TGACAGACTA CTCCTCATCC AGTGAGGAGT CCGGGACAAC AGATGAGGAA
    GACGATGATG TAGAACAAGA AGGGGCTGAG GAAGCCACCT CTGGACCAGA GGACACCAGA
    GCAGCGTCGT CCCTGAATTT GAGCAATGGT GAAACAGAAT CCGTGAAAAC CATGATTGTT
    CATGACGACG TAGAAAGTGA ACCAGCCATG ACCCCATCCA AGGAGGGCAC TCTAATCGTC
    CGCCAGAGTA CAGTTGACCA AAAGCGCGCC AGCCATCATG AGAGCAATGG CTTTGCCGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCCTCCTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTCACTA CTAATGAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGTGGGACA
    ACAGTGACTT CTGTGGTGGG ATTTTCCTGT GATGGAATGA GACCAGAAGC CATAAGGCAA
    GATCCTACCC GGAAGGGCTC AGTGGTCAAT GTGAATCCCA CCAACACTAG GCCACAGAGT
    GATACCCCGG AGATTCGTAA ATATAAGAAG AGATTTAACT CCGAGATTCT GTGTGCTGCC
    TTATGGGGAG TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT
    GGCCAAGGGA AGGTATATCC CCTGATCAAC CGAAGACGAT TTCAGCAAAT GGATGTCCTT
    GAAGGCTTGA ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT
    TTGTCCTGGT TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA
    TGGACGACTG TGGGAGATTT GGAAGGATGT GTACACTATA AAGTTGTAAA ATATGAAAGA
    ATCAAATTTC TGGTAATTGC TTTGAAGAGT TCTGTGGAGG TCTATGCGTG GGCACCCAAG
    CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGGGAAT TAGTACATAA GCCATTGCTG
    GTGGATCTCA CTGTGGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA
    TTCCATGCTG TTGATGTGGA TTCTGGATCA GTCTATGACA TTTATCTACC AACACACGTA
    AGAAAGAACC CACACTCTAT GATCCAGTGT AGCATCAAAC CCCATGCAAT CATCATCCTC
    CCCAACACAG ATGGCATGGA GCTTCTGGTG TGCTATGAAG ATGAAGGGGT TTATGTGAAT
    ACTTATGGAA GAATCACCAA GGATGTGGTT CTGCAGTGGG GAGAGATGCC AACATCTGTA
    GCATATATTC GATCCAACCA GACGATGGGC TGGGGAGAAA AGGCCATAGA GATCCGATCT
    GTGGAAACTG GTCATTTGGA TGGTGTGTTT ATGCACAAAA GGGCTCAAAG ACTAAAATTC
    CTATGTGAAC GCAATGACAA GGTCTTCTTT GCCTCTGTTC GGTCTGGTGG CAGCAGCCAG
    GTTTATTTCA TGACGTTAGG CAGGACTTCT CTTCTGAGCT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291543
    Clone ID Related Accession (Same CDS sequence) XM_022424560.1
    Accession Version XM_022424560.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGTGGTCCCA CCTGGCATCT CTCAAGAACA ATGTTTCCCC TGTCTCGCGA
    TCCCATTCCT TCAGTGACCC TTCTCCTCCC AAATTTGCAC ACCACCATCT TCGCTCTCAG
    GACCCATGTC CACCTTCCCG CAGTGAGGTG CTAAGTCAGA GTTCTGACTC TAAGTCGGAG
    GTACCTGACC CCACCCAAAA GGCTTGGTCT AGATCAGACA GTGACGAGGT GCCTCCAAGG
    GTTCCTGTGA GGACAACGTC TCGTTCCCCT GTTCTGTCCC GTCGGGATTC CCCACTGCAA
    GGCAGTGGAC AGCAAAATAG TCAAGCAGGT CAAAGAAACT CCACTAGCAG TATTGAGCCC
    CGGCTGCTGT GGGAGAGAGT GGAGAAGCTG GTGCCCAGGC CTGGCAGTGG CAGCTCCTCC
    GGATCCAGCA ACTCCGGATC CCAGCCTGGG TCCCACCCTG GGTCCCAGAG TGGCTCTGGA
    GAGCGCTTCA GAGTGAGATC ATCATCCAAA TCTGAAGGCT CTCCTTCTCA GCGCCTAGAA
    AATGCAGTGA AAAAACCTGA AGAAAAGAAA GAAGTTTTCA GACCTCTCAA GCCTGCCGGC
    GAAGTGGATT TGACTGCACT GGCCAAAGAG CTTCGAGCAG TGGAAGATGT GCGACCACCA
    CACAAAGTGA CAGACTACTC CTCATCCAGT GAGGAGTCCG GGACAACAGA TGAGGAAGAC
    GATGATGTAG AACAAGAAGG GGCTGAGGAA GCCACCTCTG GACCAGAGGA CACCAGAGCA
    GCGTCGTCCC TGAATTTGAG CAATGGTGAA ACAGAATCCG TGAAAACCAT GATTGTTCAT
    GACGACGTAG AAAGTGAACC AGCCATGACC CCATCCAAGG AGGGCACTCT AATCGTCCGC
    CAGAGTACAG TTGACCAAAA GCGCGCCAGC CATCATGAGA GCAATGGCTT TGCCGGTCGC
    ATTCACCTCT TGCCAGATCT CTTACAGCAA AGCCATTCCT CCTCCACTTC CTCCACCTCC
    TCCTCCCCAT CCTCCAGCCA GCCGACACCC ACCATGTCCC CACAGACACC CCAGGACAAG
    CTCACTACTA ATGAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC
    TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG TGGGACAACA
    GTGACTTCTG TGGTGGGATT TTCCTGTGAT GGAATGAGAC CAGAAGCCAT AAGGCAAGAT
    CCTACCCGGA AGGGCTCAGT GGTCAATGTG AATCCCACCA ACACTAGGCC ACAGAGTGAT
    ACCCCGGAGA TTCGTAAATA TAAGAAGAGA TTTAACTCCG AGATTCTGTG TGCTGCCTTA
    TGGGGAGTGA ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC
    CAAGGGAAGG TATATCCCCT GATCAACCGA AGACGATTTC AGCAAATGGA TGTCCTTGAA
    GGCTTGAATG TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG
    TCCTGGTTAA GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG
    ACGACTGTGG GAGATTTGGA AGGATGTGTA CACTATAAAG TTGTAAAATA TGAAAGAATC
    AAATTTCTGG TAATTGCTTT GAAGAGTTCT GTGGAGGTCT ATGCGTGGGC ACCCAAGCCA
    TATCACAAAT TTATGGCCTT TAAGTCATTT GGGGAATTAG TACATAAGCC ATTGCTGGTG
    GATCTCACTG TGGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC
    CATGCTGTTG ATGTGGATTC TGGATCAGTC TATGACATTT ATCTACCAAC ACACGTAAGA
    AAGAACCCAC ACTCTATGAT CCAGTGTAGC ATCAAACCCC ATGCAATCAT CATCCTCCCC
    AACACAGATG GCATGGAGCT TCTGGTGTGC TATGAAGATG AAGGGGTTTA TGTGAATACT
    TATGGAAGAA TCACCAAGGA TGTGGTTCTG CAGTGGGGAG AGATGCCAAC ATCTGTAGCA
    TATATTCGAT CCAACCAGAC GATGGGCTGG GGAGAAAAGG CCATAGAGAT CCGATCTGTG
    GAAACTGGTC ATTTGGATGG TGTGTTTATG CACAAAAGGG CTCAAAGACT AAAATTCCTA
    TGTGAACGCA ATGACAAGGT CTTCTTTGCC TCTGTTCGGT CTGGTGGCAG CAGCCAGGTT
    TATTTCATGA CGTTAGGCAG GACTTCTCTT CTGAGCTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280268.1
    CDS175..4176
    Translation

    Target ORF information:

    RefSeq Version XM_022424560.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424560.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGTGGTCCCA CCTGGCATCT CTCAAGAACA ATGTTTCCCC TGTCTCGCGA
    TCCCATTCCT TCAGTGACCC TTCTCCTCCC AAATTTGCAC ACCACCATCT TCGCTCTCAG
    GACCCATGTC CACCTTCCCG CAGTGAGGTG CTAAGTCAGA GTTCTGACTC TAAGTCGGAG
    GTACCTGACC CCACCCAAAA GGCTTGGTCT AGATCAGACA GTGACGAGGT GCCTCCAAGG
    GTTCCTGTGA GGACAACGTC TCGTTCCCCT GTTCTGTCCC GTCGGGATTC CCCACTGCAA
    GGCAGTGGAC AGCAAAATAG TCAAGCAGGT CAAAGAAACT CCACTAGCAG TATTGAGCCC
    CGGCTGCTGT GGGAGAGAGT GGAGAAGCTG GTGCCCAGGC CTGGCAGTGG CAGCTCCTCC
    GGATCCAGCA ACTCCGGATC CCAGCCTGGG TCCCACCCTG GGTCCCAGAG TGGCTCTGGA
    GAGCGCTTCA GAGTGAGATC ATCATCCAAA TCTGAAGGCT CTCCTTCTCA GCGCCTAGAA
    AATGCAGTGA AAAAACCTGA AGAAAAGAAA GAAGTTTTCA GACCTCTCAA GCCTGCCGGC
    GAAGTGGATT TGACTGCACT GGCCAAAGAG CTTCGAGCAG TGGAAGATGT GCGACCACCA
    CACAAAGTGA CAGACTACTC CTCATCCAGT GAGGAGTCCG GGACAACAGA TGAGGAAGAC
    GATGATGTAG AACAAGAAGG GGCTGAGGAA GCCACCTCTG GACCAGAGGA CACCAGAGCA
    GCGTCGTCCC TGAATTTGAG CAATGGTGAA ACAGAATCCG TGAAAACCAT GATTGTTCAT
    GACGACGTAG AAAGTGAACC AGCCATGACC CCATCCAAGG AGGGCACTCT AATCGTCCGC
    CAGAGTACAG TTGACCAAAA GCGCGCCAGC CATCATGAGA GCAATGGCTT TGCCGGTCGC
    ATTCACCTCT TGCCAGATCT CTTACAGCAA AGCCATTCCT CCTCCACTTC CTCCACCTCC
    TCCTCCCCAT CCTCCAGCCA GCCGACACCC ACCATGTCCC CACAGACACC CCAGGACAAG
    CTCACTACTA ATGAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC
    TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG TGGGACAACA
    GTGACTTCTG TGGTGGGATT TTCCTGTGAT GGAATGAGAC CAGAAGCCAT AAGGCAAGAT
    CCTACCCGGA AGGGCTCAGT GGTCAATGTG AATCCCACCA ACACTAGGCC ACAGAGTGAT
    ACCCCGGAGA TTCGTAAATA TAAGAAGAGA TTTAACTCCG AGATTCTGTG TGCTGCCTTA
    TGGGGAGTGA ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC
    CAAGGGAAGG TATATCCCCT GATCAACCGA AGACGATTTC AGCAAATGGA TGTCCTTGAA
    GGCTTGAATG TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG
    TCCTGGTTAA GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG
    ACGACTGTGG GAGATTTGGA AGGATGTGTA CACTATAAAG TTGTAAAATA TGAAAGAATC
    AAATTTCTGG TAATTGCTTT GAAGAGTTCT GTGGAGGTCT ATGCGTGGGC ACCCAAGCCA
    TATCACAAAT TTATGGCCTT TAAGTCATTT GGGGAATTAG TACATAAGCC ATTGCTGGTG
    GATCTCACTG TGGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC
    CATGCTGTTG ATGTGGATTC TGGATCAGTC TATGACATTT ATCTACCAAC ACACGTAAGA
    AAGAACCCAC ACTCTATGAT CCAGTGTAGC ATCAAACCCC ATGCAATCAT CATCCTCCCC
    AACACAGATG GCATGGAGCT TCTGGTGTGC TATGAAGATG AAGGGGTTTA TGTGAATACT
    TATGGAAGAA TCACCAAGGA TGTGGTTCTG CAGTGGGGAG AGATGCCAAC ATCTGTAGCA
    TATATTCGAT CCAACCAGAC GATGGGCTGG GGAGAAAAGG CCATAGAGAT CCGATCTGTG
    GAAACTGGTC ATTTGGATGG TGTGTTTATG CACAAAAGGG CTCAAAGACT AAAATTCCTA
    TGTGAACGCA ATGACAAGGT CTTCTTTGCC TCTGTTCGGT CTGGTGGCAG CAGCCAGGTT
    TATTTCATGA CGTTAGGCAG GACTTCTCTT CTGAGCTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291544
    Clone ID Related Accession (Same CDS sequence) XM_022424561.1
    Accession Version XM_022424561.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGGTACAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCTCCCAAAT TTGCACACCA CCATCTTCGC
    TCTCAGGACC CATGTCCACC TTCCCGCAGT GAGGTGCTAA GTCAGAGTTC TGACTCTAAG
    TCGGAGGTAC CTGACCCCAC CCAAAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT
    CCAAGGGTTC CTGTGAGGAC AACGTCTCGT TCCCCTGTTC TGTCCCGTCG GGATTCCCCA
    CTGCAAGGCA GTGGACAGCA AAATAGTCAA GCAGGTCAAA GAAACTCCAC TAGCAGTATT
    GAGCCCCGGC TGCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC
    TCCTCCGGAT CCAGCAACTC CGGATCCCAG CCTGGGTCCC ACCCTGGGTC CCAGAGTGGC
    TCTGGAGAGC GCTTCAGAGT GAGATCATCA TCCAAATCTG AAGGCTCTCC TTCTCAGCGC
    CTAGAAAATG CAGTGAAAAA ACCTGAAGAA AAGAAAGAAG TTTTCAGACC TCTCAAGCCT
    GCCGATTTGA CTGCACTGGC CAAAGAGCTT CGAGCAGTGG AAGATGTGCG ACCACCACAC
    AAAGTGACAG ACTACTCCTC ATCCAGTGAG GAGTCCGGGA CAACAGATGA GGAAGACGAT
    GATGTAGAAC AAGAAGGGGC TGAGGAAGCC ACCTCTGGAC CAGAGGACAC CAGAGCAGCG
    TCGTCCCTGA ATTTGAGCAA TGGTGAAACA GAATCCGTGA AAACCATGAT TGTTCATGAC
    GACGTAGAAA GTGAACCAGC CATGACCCCA TCCAAGGAGG GCACTCTAAT CGTCCGCCAG
    AGTACAGTTG ACCAAAAGCG CGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCATT
    CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC
    TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC
    ACTACTAATG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC
    TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGTGG GACAACAGTG
    ACTTCTGTGG TGGGATTTTC CTGTGATGGA ATGAGACCAG AAGCCATAAG GCAAGATCCT
    ACCCGGAAGG GCTCAGTGGT CAATGTGAAT CCCACCAACA CTAGGCCACA GAGTGATACC
    CCGGAGATTC GTAAATATAA GAAGAGATTT AACTCCGAGA TTCTGTGTGC TGCCTTATGG
    GGAGTGAATT TGCTAGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA
    GGGAAGGTAT ATCCCCTGAT CAACCGAAGA CGATTTCAGC AAATGGATGT CCTTGAAGGC
    TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC
    TGGTTAAGAA ATAAAATACT TCACAATGAT CCAGAAGTTG AGAAGAAGCA GGGATGGACG
    ACTGTGGGAG ATTTGGAAGG ATGTGTACAC TATAAAGTTG TAAAATATGA AAGAATCAAA
    TTTCTGGTAA TTGCTTTGAA GAGTTCTGTG GAGGTCTATG CGTGGGCACC CAAGCCATAT
    CACAAATTTA TGGCCTTTAA GTCATTTGGG GAATTAGTAC ATAAGCCATT GCTGGTGGAT
    CTCACTGTGG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT
    GCTGTTGATG TGGATTCTGG ATCAGTCTAT GACATTTATC TACCAACACA CGTAAGAAAG
    AACCCACACT CTATGATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAC
    ACAGATGGCA TGGAGCTTCT GGTGTGCTAT GAAGATGAAG GGGTTTATGT GAATACTTAT
    GGAAGAATCA CCAAGGATGT GGTTCTGCAG TGGGGAGAGA TGCCAACATC TGTAGCATAT
    ATTCGATCCA ACCAGACGAT GGGCTGGGGA GAAAAGGCCA TAGAGATCCG ATCTGTGGAA
    ACTGGTCATT TGGATGGTGT GTTTATGCAC AAAAGGGCTC AAAGACTAAA ATTCCTATGT
    GAACGCAATG ACAAGGTCTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG CCAGGTTTAT
    TTCATGACGT TAGGCAGGAC TTCTCTTCTG AGCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280269.1
    CDS175..4173
    Translation

    Target ORF information:

    RefSeq Version XM_022424561.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424561.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGGTACAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCTCCCAAAT TTGCACACCA CCATCTTCGC
    TCTCAGGACC CATGTCCACC TTCCCGCAGT GAGGTGCTAA GTCAGAGTTC TGACTCTAAG
    TCGGAGGTAC CTGACCCCAC CCAAAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT
    CCAAGGGTTC CTGTGAGGAC AACGTCTCGT TCCCCTGTTC TGTCCCGTCG GGATTCCCCA
    CTGCAAGGCA GTGGACAGCA AAATAGTCAA GCAGGTCAAA GAAACTCCAC TAGCAGTATT
    GAGCCCCGGC TGCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC
    TCCTCCGGAT CCAGCAACTC CGGATCCCAG CCTGGGTCCC ACCCTGGGTC CCAGAGTGGC
    TCTGGAGAGC GCTTCAGAGT GAGATCATCA TCCAAATCTG AAGGCTCTCC TTCTCAGCGC
    CTAGAAAATG CAGTGAAAAA ACCTGAAGAA AAGAAAGAAG TTTTCAGACC TCTCAAGCCT
    GCCGATTTGA CTGCACTGGC CAAAGAGCTT CGAGCAGTGG AAGATGTGCG ACCACCACAC
    AAAGTGACAG ACTACTCCTC ATCCAGTGAG GAGTCCGGGA CAACAGATGA GGAAGACGAT
    GATGTAGAAC AAGAAGGGGC TGAGGAAGCC ACCTCTGGAC CAGAGGACAC CAGAGCAGCG
    TCGTCCCTGA ATTTGAGCAA TGGTGAAACA GAATCCGTGA AAACCATGAT TGTTCATGAC
    GACGTAGAAA GTGAACCAGC CATGACCCCA TCCAAGGAGG GCACTCTAAT CGTCCGCCAG
    AGTACAGTTG ACCAAAAGCG CGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCATT
    CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC
    TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC
    ACTACTAATG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC
    TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGTGG GACAACAGTG
    ACTTCTGTGG TGGGATTTTC CTGTGATGGA ATGAGACCAG AAGCCATAAG GCAAGATCCT
    ACCCGGAAGG GCTCAGTGGT CAATGTGAAT CCCACCAACA CTAGGCCACA GAGTGATACC
    CCGGAGATTC GTAAATATAA GAAGAGATTT AACTCCGAGA TTCTGTGTGC TGCCTTATGG
    GGAGTGAATT TGCTAGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA
    GGGAAGGTAT ATCCCCTGAT CAACCGAAGA CGATTTCAGC AAATGGATGT CCTTGAAGGC
    TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC
    TGGTTAAGAA ATAAAATACT TCACAATGAT CCAGAAGTTG AGAAGAAGCA GGGATGGACG
    ACTGTGGGAG ATTTGGAAGG ATGTGTACAC TATAAAGTTG TAAAATATGA AAGAATCAAA
    TTTCTGGTAA TTGCTTTGAA GAGTTCTGTG GAGGTCTATG CGTGGGCACC CAAGCCATAT
    CACAAATTTA TGGCCTTTAA GTCATTTGGG GAATTAGTAC ATAAGCCATT GCTGGTGGAT
    CTCACTGTGG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT
    GCTGTTGATG TGGATTCTGG ATCAGTCTAT GACATTTATC TACCAACACA CGTAAGAAAG
    AACCCACACT CTATGATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAC
    ACAGATGGCA TGGAGCTTCT GGTGTGCTAT GAAGATGAAG GGGTTTATGT GAATACTTAT
    GGAAGAATCA CCAAGGATGT GGTTCTGCAG TGGGGAGAGA TGCCAACATC TGTAGCATAT
    ATTCGATCCA ACCAGACGAT GGGCTGGGGA GAAAAGGCCA TAGAGATCCG ATCTGTGGAA
    ACTGGTCATT TGGATGGTGT GTTTATGCAC AAAAGGGCTC AAAGACTAAA ATTCCTATGT
    GAACGCAATG ACAAGGTCTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG CCAGGTTTAT
    TTCATGACGT TAGGCAGGAC TTCTCTTCTG AGCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291545
    Clone ID Related Accession (Same CDS sequence) XM_022424562.1
    Accession Version XM_022424562.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTGCCGAT GGCGGGAGAT GGAGGAGCAC
    CGGCAGGCAG AGAGGCTCCA GAGGCAGTTG CAACAAGAAC AAGCATATCT CCTGTCTCTA
    CAGCATGACC ACAGGAGGCC GCACCCGCAG CAGCCGCCGC CACCGCAGCA GGAAAGGAGC
    AAGCCAAGCT ATCACGCTCC GGAGCCTAAG CCCCACTATG AGCCTGCTGA CAGAGCTCGA
    GAGGTGGAAG ATAGATTTAG GAAAACTAAC CACAGCTCCC CTGAAGCCCA GTCTAAGCAG
    ACAGGCAGAG TATTGGAACC ACCAGTGCCT TCCAGATCAG AGTCTTTTTC CAATGGCAAC
    TCCGAGTCTG TGCATCCTGC CCTGCAGAGA CCAGCTGAGC CACAGGTACA GTGGTCCCAC
    CTGGCATCTC TCAAGAACAA TGTTTCCCCT GTCTCGCGAT CCCATTCCTT CAGTGACCCT
    TCTCCTCCCA AATTTGCACA CCACCATCTT CGCTCTCAGG ACCCATGTCC ACCTTCCCGC
    AGTGAGGTGC TAAGTCAGAG TTCTGACTCT AAGTCGGAGG TACCTGACCC CACCCAAAAG
    GCTTGGTCTA GATCAGACAG TGACGAGGTG CCTCCAAGGG TTCCTGTGAG GACAACGTCT
    CGTTCCCCTG TTCTGTCCCG TCGGGATTCC CCACTGCAAG GCAGTGGACA GCAAAATAGT
    CAAGCAGGTC AAAGAAACTC CACTAGCAGT ATTGAGCCCC GGCTGCTGTG GGAGAGAGTG
    GAGAAGCTGG TGCCCAGGCC TGGCAGTGGC AGCTCCTCCG GATCCAGCAA CTCCGGATCC
    CAGCCTGGGT CCCACCCTGG GTCCCAGAGT GGCTCTGGAG AGCGCTTCAG AGTGAGATCA
    TCATCCAAAT CTGAAGGCTC TCCTTCTCAG CGCCTAGAAA ATGCAGTGAA AAAACCTGAA
    GAAAAGAAAG AAGTTTTCAG ACCTCTCAAG CCTGCCGGCG AAGTGGATTT GACTGCACTG
    GCCAAAGAGC TTCGAGCAGT GGAAGATGTG CGACCACCAC ACAAAGTGAC AGACTACTCC
    TCATCCAGTG AGGAGTCCGG GACAACAGAT GAGGAAGACG ATGATGTAGA ACAAGAAGGG
    GCTGAGGAAG CCACCTCTGG ACCAGAGGAC ACCAGAGCAG CGTCGTCCCT GAATTTGAGC
    AATGGTGAAA CAGAATCCGT GAAAACCATG ATTGTTCATG ACGACGTAGA AAGTGAACCA
    GCCATGACCC CATCCAAGGA GGGCACTCTA ATCGTCCGCC AGAGTACAGT TGACCAAAAG
    CGCGCCAGCC ATCATGAGAG CAATGGCTTT GCCGGTCGCA TTCACCTCTT GCCAGATCTC
    TTACAGCAAA GCCATTCCTC CTCCACTTCC TCCACCTCCT CCTCCCCATC CTCCAGCCAG
    CCGACACCCA CCATGTCCCC ACAGACACCC CAGGACAAGC TCACTACTAA TGAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGATTAC TACAGATTTC TCCATCTAGT GGGACAACAG TGACTTCTGT GGTGGGATTT
    TCCTGTGATG GAATGAGACC AGAAGCCATA AGGCAAGATC CTACCCGGAA GGGCTCAGTG
    GTCAATGTGA ATCCCACCAA CACTAGGCCA CAGAGTGATA CCCCGGAGAT TCGTAAATAT
    AAGAAGAGAT TTAACTCCGA GATTCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG
    GGTACAGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT ATATCCCCTG
    ATCAACCGAA GACGATTTCA GCAAATGGAT GTCCTTGAAG GCTTGAATGT CTTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA
    CTTCACAATG ATCCAGAAGT TGAGAAGAAG CAGGGATGGA CGACTGTGGG AGATTTGGAA
    GGATGTGTAC ACTATAAAGT TGTAAAATAT GAAAGAATCA AATTTCTGGT AATTGCTTTG
    AAGAGTTCTG TGGAGGTCTA TGCGTGGGCA CCCAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GGGAATTAGT ACATAAGCCA TTGCTGGTGG ATCTCACTGT GGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCT
    GGATCAGTCT ATGACATTTA TCTACCAACA CACGTAAGAA AGAACCCACA CTCTATGATC
    CAGTGTAGCA TCAAACCCCA TGCAATCATC ATCCTCCCCA ACACAGATGG CATGGAGCTT
    CTGGTGTGCT ATGAAGATGA AGGGGTTTAT GTGAATACTT ATGGAAGAAT CACCAAGGAT
    GTGGTTCTGC AGTGGGGAGA GATGCCAACA TCTGTAGCAT ATATTCGATC CAACCAGACG
    ATGGGCTGGG GAGAAAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA TTTGGATGGT
    GTGTTTATGC ACAAAAGGGC TCAAAGACTA AAATTCCTAT GTGAACGCAA TGACAAGGTC
    TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC AGCCAGGTTT ATTTCATGAC GTTAGGCAGG
    ACTTCTCTTC TGAGCTGGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280270.1
    CDS175..4095
    Translation

    Target ORF information:

    RefSeq Version XM_022424562.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424562.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTGCCGAT GGCGGGAGAT GGAGGAGCAC
    CGGCAGGCAG AGAGGCTCCA GAGGCAGTTG CAACAAGAAC AAGCATATCT CCTGTCTCTA
    CAGCATGACC ACAGGAGGCC GCACCCGCAG CAGCCGCCGC CACCGCAGCA GGAAAGGAGC
    AAGCCAAGCT ATCACGCTCC GGAGCCTAAG CCCCACTATG AGCCTGCTGA CAGAGCTCGA
    GAGGTGGAAG ATAGATTTAG GAAAACTAAC CACAGCTCCC CTGAAGCCCA GTCTAAGCAG
    ACAGGCAGAG TATTGGAACC ACCAGTGCCT TCCAGATCAG AGTCTTTTTC CAATGGCAAC
    TCCGAGTCTG TGCATCCTGC CCTGCAGAGA CCAGCTGAGC CACAGGTACA GTGGTCCCAC
    CTGGCATCTC TCAAGAACAA TGTTTCCCCT GTCTCGCGAT CCCATTCCTT CAGTGACCCT
    TCTCCTCCCA AATTTGCACA CCACCATCTT CGCTCTCAGG ACCCATGTCC ACCTTCCCGC
    AGTGAGGTGC TAAGTCAGAG TTCTGACTCT AAGTCGGAGG TACCTGACCC CACCCAAAAG
    GCTTGGTCTA GATCAGACAG TGACGAGGTG CCTCCAAGGG TTCCTGTGAG GACAACGTCT
    CGTTCCCCTG TTCTGTCCCG TCGGGATTCC CCACTGCAAG GCAGTGGACA GCAAAATAGT
    CAAGCAGGTC AAAGAAACTC CACTAGCAGT ATTGAGCCCC GGCTGCTGTG GGAGAGAGTG
    GAGAAGCTGG TGCCCAGGCC TGGCAGTGGC AGCTCCTCCG GATCCAGCAA CTCCGGATCC
    CAGCCTGGGT CCCACCCTGG GTCCCAGAGT GGCTCTGGAG AGCGCTTCAG AGTGAGATCA
    TCATCCAAAT CTGAAGGCTC TCCTTCTCAG CGCCTAGAAA ATGCAGTGAA AAAACCTGAA
    GAAAAGAAAG AAGTTTTCAG ACCTCTCAAG CCTGCCGGCG AAGTGGATTT GACTGCACTG
    GCCAAAGAGC TTCGAGCAGT GGAAGATGTG CGACCACCAC ACAAAGTGAC AGACTACTCC
    TCATCCAGTG AGGAGTCCGG GACAACAGAT GAGGAAGACG ATGATGTAGA ACAAGAAGGG
    GCTGAGGAAG CCACCTCTGG ACCAGAGGAC ACCAGAGCAG CGTCGTCCCT GAATTTGAGC
    AATGGTGAAA CAGAATCCGT GAAAACCATG ATTGTTCATG ACGACGTAGA AAGTGAACCA
    GCCATGACCC CATCCAAGGA GGGCACTCTA ATCGTCCGCC AGAGTACAGT TGACCAAAAG
    CGCGCCAGCC ATCATGAGAG CAATGGCTTT GCCGGTCGCA TTCACCTCTT GCCAGATCTC
    TTACAGCAAA GCCATTCCTC CTCCACTTCC TCCACCTCCT CCTCCCCATC CTCCAGCCAG
    CCGACACCCA CCATGTCCCC ACAGACACCC CAGGACAAGC TCACTACTAA TGAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGATTAC TACAGATTTC TCCATCTAGT GGGACAACAG TGACTTCTGT GGTGGGATTT
    TCCTGTGATG GAATGAGACC AGAAGCCATA AGGCAAGATC CTACCCGGAA GGGCTCAGTG
    GTCAATGTGA ATCCCACCAA CACTAGGCCA CAGAGTGATA CCCCGGAGAT TCGTAAATAT
    AAGAAGAGAT TTAACTCCGA GATTCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG
    GGTACAGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT ATATCCCCTG
    ATCAACCGAA GACGATTTCA GCAAATGGAT GTCCTTGAAG GCTTGAATGT CTTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA
    CTTCACAATG ATCCAGAAGT TGAGAAGAAG CAGGGATGGA CGACTGTGGG AGATTTGGAA
    GGATGTGTAC ACTATAAAGT TGTAAAATAT GAAAGAATCA AATTTCTGGT AATTGCTTTG
    AAGAGTTCTG TGGAGGTCTA TGCGTGGGCA CCCAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GGGAATTAGT ACATAAGCCA TTGCTGGTGG ATCTCACTGT GGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCT
    GGATCAGTCT ATGACATTTA TCTACCAACA CACGTAAGAA AGAACCCACA CTCTATGATC
    CAGTGTAGCA TCAAACCCCA TGCAATCATC ATCCTCCCCA ACACAGATGG CATGGAGCTT
    CTGGTGTGCT ATGAAGATGA AGGGGTTTAT GTGAATACTT ATGGAAGAAT CACCAAGGAT
    GTGGTTCTGC AGTGGGGAGA GATGCCAACA TCTGTAGCAT ATATTCGATC CAACCAGACG
    ATGGGCTGGG GAGAAAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA TTTGGATGGT
    GTGTTTATGC ACAAAAGGGC TCAAAGACTA AAATTCCTAT GTGAACGCAA TGACAAGGTC
    TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC AGCCAGGTTT ATTTCATGAC GTTAGGCAGG
    ACTTCTCTTC TGAGCTGGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291546
    Clone ID Related Accession (Same CDS sequence) XM_022424563.1
    Accession Version XM_022424563.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3915bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGCAT
    GACCACAGGA GGCCGCACCC GCAGCAGCCG CCGCCACCGC AGCAGGAAAG GAGCAAGCCA
    AGCTATCACG CTCCGGAGCC TAAGCCCCAC TATGAGCCTG CTGACAGAGC TCGAGAGGTG
    GAAGATAGAT TTAGGAAAAC TAACCACAGC TCCCCTGAAG CCCAGTCTAA GCAGACAGGC
    AGAGTATTGG AACCACCAGT GCCTTCCAGA TCAGAGTCTT TTTCCAATGG CAACTCCGAG
    TCTGTGCATC CTGCCCTGCA GAGACCAGCT GAGCCACAGG TACAGTGGTC CCACCTGGCA
    TCTCTCAAGA ACAATGTTTC CCCTGTCTCG CGATCCCATT CCTTCAGTGA CCCTTCTCCT
    CCCAAATTTG CACACCACCA TCTTCGCTCT CAGGACCCAT GTCCACCTTC CCGCAGTGAG
    GTGCTAAGTC AGAGTTCTGA CTCTAAGTCG GAGGTACCTG ACCCCACCCA AAAGGCTTGG
    TCTAGATCAG ACAGTGACGA GGTGCCTCCA AGGGTTCCTG TGAGGACAAC GTCTCGTTCC
    CCTGTTCTGT CCCGTCGGGA TTCCCCACTG CAAGGCAGTG GACAGCAAAA TAGTCAAGCA
    GGTCAAAGAA ACTCCACTAG CAGTATTGAG CCCCGGCTGC TGTGGGAGAG AGTGGAGAAG
    CTGGTGCCCA GGCCTGGCAG TGGCAGCTCC TCCGGATCCA GCAACTCCGG ATCCCAGCCT
    GGGTCCCACC CTGGGTCCCA GAGTGGCTCT GGAGAGCGCT TCAGAGTGAG ATCATCATCC
    AAATCTGAAG GCTCTCCTTC TCAGCGCCTA GAAAATGCAG TGAAAAAACC TGAAGAAAAG
    AAAGAAGTTT TCAGACCTCT CAAGCCTGCC GGCGAAGTGG ATTTGACTGC ACTGGCCAAA
    GAGCTTCGAG CAGTGGAAGA TGTGCGACCA CCACACAAAG TGACAGACTA CTCCTCATCC
    AGTGAGGAGT CCGGGACAAC AGATGAGGAA GACGATGATG TAGAACAAGA AGGGGCTGAG
    GAAGCCACCT CTGGACCAGA GGACACCAGA GCAGCGTCGT CCCTGAATTT GAGCAATGGT
    GAAACAGAAT CCGTGAAAAC CATGATTGTT CATGACGACG TAGAAAGTGA ACCAGCCATG
    ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGAGTA CAGTTGACCA AAAGCGCGCC
    AGCCATCATG AGAGCAATGG CTTTGCCGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG
    CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCCTCCC CATCCTCCAG CCAGCCGACA
    CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTA CTAATGAGAC TCAGTCCGCT
    AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA
    TTACTACAGA TTTCTCCATC TAGTGGGACA ACAGTGACTT CTGTGGTGGG ATTTTCCTGT
    GATGGAATGA GACCAGAAGC CATAAGGCAA GATCCTACCC GGAAGGGCTC AGTGGTCAAT
    GTGAATCCCA CCAACACTAG GCCACAGAGT GATACCCCGG AGATTCGTAA ATATAAGAAG
    AGATTTAACT CCGAGATTCT GTGTGCTGCC TTATGGGGAG TGAATTTGCT AGTGGGTACA
    GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTATATCC CCTGATCAAC
    CGAAGACGAT TTCAGCAAAT GGATGTCCTT GAAGGCTTGA ATGTCTTGGT GACAATATCT
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC
    AATGATCCAG AAGTTGAGAA GAAGCAGGGA TGGACGACTG TGGGAGATTT GGAAGGATGT
    GTACACTATA AAGTTGTAAA ATATGAAAGA ATCAAATTTC TGGTAATTGC TTTGAAGAGT
    TCTGTGGAGG TCTATGCGTG GGCACCCAAG CCATATCACA AATTTATGGC CTTTAAGTCA
    TTTGGGGAAT TAGTACATAA GCCATTGCTG GTGGATCTCA CTGTGGAGGA AGGCCAGAGG
    TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA TTCTGGATCA
    GTCTATGACA TTTATCTACC AACACACGTA AGAAAGAACC CACACTCTAT GATCCAGTGT
    AGCATCAAAC CCCATGCAAT CATCATCCTC CCCAACACAG ATGGCATGGA GCTTCTGGTG
    TGCTATGAAG ATGAAGGGGT TTATGTGAAT ACTTATGGAA GAATCACCAA GGATGTGGTT
    CTGCAGTGGG GAGAGATGCC AACATCTGTA GCATATATTC GATCCAACCA GACGATGGGC
    TGGGGAGAAA AGGCCATAGA GATCCGATCT GTGGAAACTG GTCATTTGGA TGGTGTGTTT
    ATGCACAAAA GGGCTCAAAG ACTAAAATTC CTATGTGAAC GCAATGACAA GGTCTTCTTT
    GCCTCTGTTC GGTCTGGTGG CAGCAGCCAG GTTTATTTCA TGACGTTAGG CAGGACTTCT
    CTTCTGAGCT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280271.1
    CDS175..4089
    Translation

    Target ORF information:

    RefSeq Version XM_022424563.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424563.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGCAT
    GACCACAGGA GGCCGCACCC GCAGCAGCCG CCGCCACCGC AGCAGGAAAG GAGCAAGCCA
    AGCTATCACG CTCCGGAGCC TAAGCCCCAC TATGAGCCTG CTGACAGAGC TCGAGAGGTG
    GAAGATAGAT TTAGGAAAAC TAACCACAGC TCCCCTGAAG CCCAGTCTAA GCAGACAGGC
    AGAGTATTGG AACCACCAGT GCCTTCCAGA TCAGAGTCTT TTTCCAATGG CAACTCCGAG
    TCTGTGCATC CTGCCCTGCA GAGACCAGCT GAGCCACAGG TACAGTGGTC CCACCTGGCA
    TCTCTCAAGA ACAATGTTTC CCCTGTCTCG CGATCCCATT CCTTCAGTGA CCCTTCTCCT
    CCCAAATTTG CACACCACCA TCTTCGCTCT CAGGACCCAT GTCCACCTTC CCGCAGTGAG
    GTGCTAAGTC AGAGTTCTGA CTCTAAGTCG GAGGTACCTG ACCCCACCCA AAAGGCTTGG
    TCTAGATCAG ACAGTGACGA GGTGCCTCCA AGGGTTCCTG TGAGGACAAC GTCTCGTTCC
    CCTGTTCTGT CCCGTCGGGA TTCCCCACTG CAAGGCAGTG GACAGCAAAA TAGTCAAGCA
    GGTCAAAGAA ACTCCACTAG CAGTATTGAG CCCCGGCTGC TGTGGGAGAG AGTGGAGAAG
    CTGGTGCCCA GGCCTGGCAG TGGCAGCTCC TCCGGATCCA GCAACTCCGG ATCCCAGCCT
    GGGTCCCACC CTGGGTCCCA GAGTGGCTCT GGAGAGCGCT TCAGAGTGAG ATCATCATCC
    AAATCTGAAG GCTCTCCTTC TCAGCGCCTA GAAAATGCAG TGAAAAAACC TGAAGAAAAG
    AAAGAAGTTT TCAGACCTCT CAAGCCTGCC GGCGAAGTGG ATTTGACTGC ACTGGCCAAA
    GAGCTTCGAG CAGTGGAAGA TGTGCGACCA CCACACAAAG TGACAGACTA CTCCTCATCC
    AGTGAGGAGT CCGGGACAAC AGATGAGGAA GACGATGATG TAGAACAAGA AGGGGCTGAG
    GAAGCCACCT CTGGACCAGA GGACACCAGA GCAGCGTCGT CCCTGAATTT GAGCAATGGT
    GAAACAGAAT CCGTGAAAAC CATGATTGTT CATGACGACG TAGAAAGTGA ACCAGCCATG
    ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGAGTA CAGTTGACCA AAAGCGCGCC
    AGCCATCATG AGAGCAATGG CTTTGCCGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG
    CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCCTCCC CATCCTCCAG CCAGCCGACA
    CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTA CTAATGAGAC TCAGTCCGCT
    AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA
    TTACTACAGA TTTCTCCATC TAGTGGGACA ACAGTGACTT CTGTGGTGGG ATTTTCCTGT
    GATGGAATGA GACCAGAAGC CATAAGGCAA GATCCTACCC GGAAGGGCTC AGTGGTCAAT
    GTGAATCCCA CCAACACTAG GCCACAGAGT GATACCCCGG AGATTCGTAA ATATAAGAAG
    AGATTTAACT CCGAGATTCT GTGTGCTGCC TTATGGGGAG TGAATTTGCT AGTGGGTACA
    GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTATATCC CCTGATCAAC
    CGAAGACGAT TTCAGCAAAT GGATGTCCTT GAAGGCTTGA ATGTCTTGGT GACAATATCT
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC
    AATGATCCAG AAGTTGAGAA GAAGCAGGGA TGGACGACTG TGGGAGATTT GGAAGGATGT
    GTACACTATA AAGTTGTAAA ATATGAAAGA ATCAAATTTC TGGTAATTGC TTTGAAGAGT
    TCTGTGGAGG TCTATGCGTG GGCACCCAAG CCATATCACA AATTTATGGC CTTTAAGTCA
    TTTGGGGAAT TAGTACATAA GCCATTGCTG GTGGATCTCA CTGTGGAGGA AGGCCAGAGG
    TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA TTCTGGATCA
    GTCTATGACA TTTATCTACC AACACACGTA AGAAAGAACC CACACTCTAT GATCCAGTGT
    AGCATCAAAC CCCATGCAAT CATCATCCTC CCCAACACAG ATGGCATGGA GCTTCTGGTG
    TGCTATGAAG ATGAAGGGGT TTATGTGAAT ACTTATGGAA GAATCACCAA GGATGTGGTT
    CTGCAGTGGG GAGAGATGCC AACATCTGTA GCATATATTC GATCCAACCA GACGATGGGC
    TGGGGAGAAA AGGCCATAGA GATCCGATCT GTGGAAACTG GTCATTTGGA TGGTGTGTTT
    ATGCACAAAA GGGCTCAAAG ACTAAAATTC CTATGTGAAC GCAATGACAA GGTCTTCTTT
    GCCTCTGTTC GGTCTGGTGG CAGCAGCCAG GTTTATTTCA TGACGTTAGG CAGGACTTCT
    CTTCTGAGCT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291547
    Clone ID Related Accession (Same CDS sequence) XM_022424564.1
    Accession Version XM_022424564.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTACAGTGGT CCCACCTGGC ATCTCTCAAG
    AACAATGTTT CCCCTGTCTC GCGATCCCAT TCCTTCAGTG ACCCTTCTCC TCCCAAATTT
    GCACACCACC ATCTTCGCTC TCAGGACCCA TGTCCACCTT CCCGCAGTGA GGTGCTAAGT
    CAGAGTTCTG ACTCTAAGTC GGAGGTACCT GACCCCACCC AAAAGGCTTG GTCTAGATCA
    GACAGTGACG AGGTGCCTCC AAGGGTTCCT GTGAGGACAA CGTCTCGTTC CCCTGTTCTG
    TCCCGTCGGG ATTCCCCACT GCAAGGCAGT GGACAGCAAA ATAGTCAAGC AGGTCAAAGA
    AACTCCACTA GCAGTATTGA GCCCCGGCTG CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC
    AGGCCTGGCA GTGGCAGCTC CTCCGGATCC AGCAACTCCG GATCCCAGCC TGGGTCCCAC
    CCTGGGTCCC AGAGTGGCTC TGGAGAGCGC TTCAGAGTGA GATCATCATC CAAATCTGAA
    GGCTCTCCTT CTCAGCGCCT AGAAAATGCA GTGAAAAAAC CTGAAGAAAA GAAAGAAGTT
    TTCAGACCTC TCAAGCCTGC CGGCGAAGTG GATTTGACTG CACTGGCCAA AGAGCTTCGA
    GCAGTGGAAG ATGTGCGACC ACCACACAAA GTGACAGACT ACTCCTCATC CAGTGAGGAG
    TCCGGGACAA CAGATGAGGA AGACGATGAT GTAGAACAAG AAGGGGCTGA GGAAGCCACC
    TCTGGACCAG AGGACACCAG AGCAGCGTCG TCCCTGAATT TGAGCAATGG TGAAACAGAA
    TCCGTGAAAA CCATGATTGT TCATGACGAC GTAGAAAGTG AACCAGCCAT GACCCCATCC
    AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGCGC CAGCCATCAT
    GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT
    TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG
    TCCCCACAGA CACCCCAGGA CAAGCTCACT ACTAATGAGA CTCAGTCCGC TAGTAGCACA
    CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG
    ATTTCTCCAT CTAGTGGGAC AACAGTGACT TCTGTGGTGG GATTTTCCTG TGATGGAATG
    AGACCAGAAG CCATAAGGCA AGATCCTACC CGGAAGGGCT CAGTGGTCAA TGTGAATCCC
    ACCAACACTA GGCCACAGAG TGATACCCCG GAGATTCGTA AATATAAGAA GAGATTTAAC
    TCCGAGATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC
    CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTATATC CCCTGATCAA CCGAAGACGA
    TTTCAGCAAA TGGATGTCCT TGAAGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG
    GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA
    GAAGTTGAGA AGAAGCAGGG ATGGACGACT GTGGGAGATT TGGAAGGATG TGTACACTAT
    AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTAATTG CTTTGAAGAG TTCTGTGGAG
    GTCTATGCGT GGGCACCCAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGGGAA
    TTAGTACATA AGCCATTGCT GGTGGATCTC ACTGTGGAGG AAGGCCAGAG GTTGAAAGTG
    ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCTGGATC AGTCTATGAC
    ATTTATCTAC CAACACACGT AAGAAAGAAC CCACACTCTA TGATCCAGTG TAGCATCAAA
    CCCCATGCAA TCATCATCCT CCCCAACACA GATGGCATGG AGCTTCTGGT GTGCTATGAA
    GATGAAGGGG TTTATGTGAA TACTTATGGA AGAATCACCA AGGATGTGGT TCTGCAGTGG
    GGAGAGATGC CAACATCTGT AGCATATATT CGATCCAACC AGACGATGGG CTGGGGAGAA
    AAGGCCATAG AGATCCGATC TGTGGAAACT GGTCATTTGG ATGGTGTGTT TATGCACAAA
    AGGGCTCAAA GACTAAAATT CCTATGTGAA CGCAATGACA AGGTCTTCTT TGCCTCTGTT
    CGGTCTGGTG GCAGCAGCCA GGTTTATTTC ATGACGTTAG GCAGGACTTC TCTTCTGAGC
    TGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280272.1
    CDS175..4020
    Translation

    Target ORF information:

    RefSeq Version XM_022424564.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424564.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTACAGTGGT CCCACCTGGC ATCTCTCAAG
    AACAATGTTT CCCCTGTCTC GCGATCCCAT TCCTTCAGTG ACCCTTCTCC TCCCAAATTT
    GCACACCACC ATCTTCGCTC TCAGGACCCA TGTCCACCTT CCCGCAGTGA GGTGCTAAGT
    CAGAGTTCTG ACTCTAAGTC GGAGGTACCT GACCCCACCC AAAAGGCTTG GTCTAGATCA
    GACAGTGACG AGGTGCCTCC AAGGGTTCCT GTGAGGACAA CGTCTCGTTC CCCTGTTCTG
    TCCCGTCGGG ATTCCCCACT GCAAGGCAGT GGACAGCAAA ATAGTCAAGC AGGTCAAAGA
    AACTCCACTA GCAGTATTGA GCCCCGGCTG CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC
    AGGCCTGGCA GTGGCAGCTC CTCCGGATCC AGCAACTCCG GATCCCAGCC TGGGTCCCAC
    CCTGGGTCCC AGAGTGGCTC TGGAGAGCGC TTCAGAGTGA GATCATCATC CAAATCTGAA
    GGCTCTCCTT CTCAGCGCCT AGAAAATGCA GTGAAAAAAC CTGAAGAAAA GAAAGAAGTT
    TTCAGACCTC TCAAGCCTGC CGGCGAAGTG GATTTGACTG CACTGGCCAA AGAGCTTCGA
    GCAGTGGAAG ATGTGCGACC ACCACACAAA GTGACAGACT ACTCCTCATC CAGTGAGGAG
    TCCGGGACAA CAGATGAGGA AGACGATGAT GTAGAACAAG AAGGGGCTGA GGAAGCCACC
    TCTGGACCAG AGGACACCAG AGCAGCGTCG TCCCTGAATT TGAGCAATGG TGAAACAGAA
    TCCGTGAAAA CCATGATTGT TCATGACGAC GTAGAAAGTG AACCAGCCAT GACCCCATCC
    AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGCGC CAGCCATCAT
    GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT
    TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG
    TCCCCACAGA CACCCCAGGA CAAGCTCACT ACTAATGAGA CTCAGTCCGC TAGTAGCACA
    CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG
    ATTTCTCCAT CTAGTGGGAC AACAGTGACT TCTGTGGTGG GATTTTCCTG TGATGGAATG
    AGACCAGAAG CCATAAGGCA AGATCCTACC CGGAAGGGCT CAGTGGTCAA TGTGAATCCC
    ACCAACACTA GGCCACAGAG TGATACCCCG GAGATTCGTA AATATAAGAA GAGATTTAAC
    TCCGAGATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC
    CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTATATC CCCTGATCAA CCGAAGACGA
    TTTCAGCAAA TGGATGTCCT TGAAGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG
    GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA
    GAAGTTGAGA AGAAGCAGGG ATGGACGACT GTGGGAGATT TGGAAGGATG TGTACACTAT
    AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTAATTG CTTTGAAGAG TTCTGTGGAG
    GTCTATGCGT GGGCACCCAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGGGAA
    TTAGTACATA AGCCATTGCT GGTGGATCTC ACTGTGGAGG AAGGCCAGAG GTTGAAAGTG
    ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCTGGATC AGTCTATGAC
    ATTTATCTAC CAACACACGT AAGAAAGAAC CCACACTCTA TGATCCAGTG TAGCATCAAA
    CCCCATGCAA TCATCATCCT CCCCAACACA GATGGCATGG AGCTTCTGGT GTGCTATGAA
    GATGAAGGGG TTTATGTGAA TACTTATGGA AGAATCACCA AGGATGTGGT TCTGCAGTGG
    GGAGAGATGC CAACATCTGT AGCATATATT CGATCCAACC AGACGATGGG CTGGGGAGAA
    AAGGCCATAG AGATCCGATC TGTGGAAACT GGTCATTTGG ATGGTGTGTT TATGCACAAA
    AGGGCTCAAA GACTAAAATT CCTATGTGAA CGCAATGACA AGGTCTTCTT TGCCTCTGTT
    CGGTCTGGTG GCAGCAGCCA GGTTTATTTC ATGACGTTAG GCAGGACTTC TCTTCTGAGC
    TGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291548
    Clone ID Related Accession (Same CDS sequence) XM_022424565.1
    Accession Version XM_022424565.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3840bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG TGGTCCCACC TGGCATCTCT CAAGAACAAT
    GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCTCCCAA ATTTGCACAC
    CACCATCTTC GCTCTCAGGA CCCATGTCCA CCTTCCCGCA GTGAGGTGCT AAGTCAGAGT
    TCTGACTCTA AGTCGGAGGT ACCTGACCCC ACCCAAAAGG CTTGGTCTAG ATCAGACAGT
    GACGAGGTGC CTCCAAGGGT TCCTGTGAGG ACAACGTCTC GTTCCCCTGT TCTGTCCCGT
    CGGGATTCCC CACTGCAAGG CAGTGGACAG CAAAATAGTC AAGCAGGTCA AAGAAACTCC
    ACTAGCAGTA TTGAGCCCCG GCTGCTGTGG GAGAGAGTGG AGAAGCTGGT GCCCAGGCCT
    GGCAGTGGCA GCTCCTCCGG ATCCAGCAAC TCCGGATCCC AGCCTGGGTC CCACCCTGGG
    TCCCAGAGTG GCTCTGGAGA GCGCTTCAGA GTGAGATCAT CATCCAAATC TGAAGGCTCT
    CCTTCTCAGC GCCTAGAAAA TGCAGTGAAA AAACCTGAAG AAAAGAAAGA AGTTTTCAGA
    CCTCTCAAGC CTGCCGGCGA AGTGGATTTG ACTGCACTGG CCAAAGAGCT TCGAGCAGTG
    GAAGATGTGC GACCACCACA CAAAGTGACA GACTACTCCT CATCCAGTGA GGAGTCCGGG
    ACAACAGATG AGGAAGACGA TGATGTAGAA CAAGAAGGGG CTGAGGAAGC CACCTCTGGA
    CCAGAGGACA CCAGAGCAGC GTCGTCCCTG AATTTGAGCA ATGGTGAAAC AGAATCCGTG
    AAAACCATGA TTGTTCATGA CGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG
    GGCACTCTAA TCGTCCGCCA GAGTACAGTT GACCAAAAGC GCGCCAGCCA TCATGAGAGC
    AATGGCTTTG CCGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC
    TCCACTTCCT CCACCTCCTC CTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA
    CAGACACCCC AGGACAAGCT CACTACTAAT GAGACTCAGT CCGCTAGTAG CACACTCCAG
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT
    CCATCTAGTG GGACAACAGT GACTTCTGTG GTGGGATTTT CCTGTGATGG AATGAGACCA
    GAAGCCATAA GGCAAGATCC TACCCGGAAG GGCTCAGTGG TCAATGTGAA TCCCACCAAC
    ACTAGGCCAC AGAGTGATAC CCCGGAGATT CGTAAATATA AGAAGAGATT TAACTCCGAG
    ATTCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTGG GTACAGAGAG TGGCCTGATG
    CTGCTGGACA GAAGTGGCCA AGGGAAGGTA TATCCCCTGA TCAACCGAAG ACGATTTCAG
    CAAATGGATG TCCTTGAAGG CTTGAATGTC TTGGTGACAA TATCTGGCAA AAAGGATAAG
    TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAATGA TCCAGAAGTT
    GAGAAGAAGC AGGGATGGAC GACTGTGGGA GATTTGGAAG GATGTGTACA CTATAAAGTT
    GTAAAATATG AAAGAATCAA ATTTCTGGTA ATTGCTTTGA AGAGTTCTGT GGAGGTCTAT
    GCGTGGGCAC CCAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG GGAATTAGTA
    CATAAGCCAT TGCTGGTGGA TCTCACTGTG GAGGAAGGCC AGAGGTTGAA AGTGATCTAT
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCTG GATCAGTCTA TGACATTTAT
    CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATCC AGTGTAGCAT CAAACCCCAT
    GCAATCATCA TCCTCCCCAA CACAGATGGC ATGGAGCTTC TGGTGTGCTA TGAAGATGAA
    GGGGTTTATG TGAATACTTA TGGAAGAATC ACCAAGGATG TGGTTCTGCA GTGGGGAGAG
    ATGCCAACAT CTGTAGCATA TATTCGATCC AACCAGACGA TGGGCTGGGG AGAAAAGGCC
    ATAGAGATCC GATCTGTGGA AACTGGTCAT TTGGATGGTG TGTTTATGCA CAAAAGGGCT
    CAAAGACTAA AATTCCTATG TGAACGCAAT GACAAGGTCT TCTTTGCCTC TGTTCGGTCT
    GGTGGCAGCA GCCAGGTTTA TTTCATGACG TTAGGCAGGA CTTCTCTTCT GAGCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280273.1
    CDS175..4014
    Translation

    Target ORF information:

    RefSeq Version XM_022424565.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424565.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG TGGTCCCACC TGGCATCTCT CAAGAACAAT
    GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCTCCCAA ATTTGCACAC
    CACCATCTTC GCTCTCAGGA CCCATGTCCA CCTTCCCGCA GTGAGGTGCT AAGTCAGAGT
    TCTGACTCTA AGTCGGAGGT ACCTGACCCC ACCCAAAAGG CTTGGTCTAG ATCAGACAGT
    GACGAGGTGC CTCCAAGGGT TCCTGTGAGG ACAACGTCTC GTTCCCCTGT TCTGTCCCGT
    CGGGATTCCC CACTGCAAGG CAGTGGACAG CAAAATAGTC AAGCAGGTCA AAGAAACTCC
    ACTAGCAGTA TTGAGCCCCG GCTGCTGTGG GAGAGAGTGG AGAAGCTGGT GCCCAGGCCT
    GGCAGTGGCA GCTCCTCCGG ATCCAGCAAC TCCGGATCCC AGCCTGGGTC CCACCCTGGG
    TCCCAGAGTG GCTCTGGAGA GCGCTTCAGA GTGAGATCAT CATCCAAATC TGAAGGCTCT
    CCTTCTCAGC GCCTAGAAAA TGCAGTGAAA AAACCTGAAG AAAAGAAAGA AGTTTTCAGA
    CCTCTCAAGC CTGCCGGCGA AGTGGATTTG ACTGCACTGG CCAAAGAGCT TCGAGCAGTG
    GAAGATGTGC GACCACCACA CAAAGTGACA GACTACTCCT CATCCAGTGA GGAGTCCGGG
    ACAACAGATG AGGAAGACGA TGATGTAGAA CAAGAAGGGG CTGAGGAAGC CACCTCTGGA
    CCAGAGGACA CCAGAGCAGC GTCGTCCCTG AATTTGAGCA ATGGTGAAAC AGAATCCGTG
    AAAACCATGA TTGTTCATGA CGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG
    GGCACTCTAA TCGTCCGCCA GAGTACAGTT GACCAAAAGC GCGCCAGCCA TCATGAGAGC
    AATGGCTTTG CCGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC
    TCCACTTCCT CCACCTCCTC CTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA
    CAGACACCCC AGGACAAGCT CACTACTAAT GAGACTCAGT CCGCTAGTAG CACACTCCAG
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT
    CCATCTAGTG GGACAACAGT GACTTCTGTG GTGGGATTTT CCTGTGATGG AATGAGACCA
    GAAGCCATAA GGCAAGATCC TACCCGGAAG GGCTCAGTGG TCAATGTGAA TCCCACCAAC
    ACTAGGCCAC AGAGTGATAC CCCGGAGATT CGTAAATATA AGAAGAGATT TAACTCCGAG
    ATTCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTGG GTACAGAGAG TGGCCTGATG
    CTGCTGGACA GAAGTGGCCA AGGGAAGGTA TATCCCCTGA TCAACCGAAG ACGATTTCAG
    CAAATGGATG TCCTTGAAGG CTTGAATGTC TTGGTGACAA TATCTGGCAA AAAGGATAAG
    TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAATGA TCCAGAAGTT
    GAGAAGAAGC AGGGATGGAC GACTGTGGGA GATTTGGAAG GATGTGTACA CTATAAAGTT
    GTAAAATATG AAAGAATCAA ATTTCTGGTA ATTGCTTTGA AGAGTTCTGT GGAGGTCTAT
    GCGTGGGCAC CCAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG GGAATTAGTA
    CATAAGCCAT TGCTGGTGGA TCTCACTGTG GAGGAAGGCC AGAGGTTGAA AGTGATCTAT
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCTG GATCAGTCTA TGACATTTAT
    CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATCC AGTGTAGCAT CAAACCCCAT
    GCAATCATCA TCCTCCCCAA CACAGATGGC ATGGAGCTTC TGGTGTGCTA TGAAGATGAA
    GGGGTTTATG TGAATACTTA TGGAAGAATC ACCAAGGATG TGGTTCTGCA GTGGGGAGAG
    ATGCCAACAT CTGTAGCATA TATTCGATCC AACCAGACGA TGGGCTGGGG AGAAAAGGCC
    ATAGAGATCC GATCTGTGGA AACTGGTCAT TTGGATGGTG TGTTTATGCA CAAAAGGGCT
    CAAAGACTAA AATTCCTATG TGAACGCAAT GACAAGGTCT TCTTTGCCTC TGTTCGGTCT
    GGTGGCAGCA GCCAGGTTTA TTTCATGACG TTAGGCAGGA CTTCTCTTCT GAGCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291549
    Clone ID Related Accession (Same CDS sequence) XM_022424566.1
    Accession Version XM_022424566.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3816bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGGTACAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCTCCCAAAT TTGCACACCA CCATCTTCGC
    TCTCAGGACC CATGTCCACC TTCCCGCAGT GAGGTGCTAA GTCAGAGTTC TGACTCTAAG
    TCGGAGGTAC CTGACCCCAC CCAAAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT
    CCAAGGGTTC CTGTGAGGAC AACGTCTCGT TCCCCTGTTC TGTCCCGTCG GGATTCCCCA
    CTGCAAGGCA GTGGACAGCA AAATAGTCAA GCAGGTCAAA GAAACTCCAC TAGCAGTATT
    GAGCCCCGGC TGCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC
    TCCTCCGGAT CCAGCAACTC CGGATCCCAG CCTGGGTCCC ACCCTGGGTC CCAGAGTGGC
    TCTGGAGAGC GCTTCAGAGT GAGATCATCA TCCAAATCTG AAGGCTCTCC TTCTCAGCGC
    CTAGAAAATG CAGTGAAAAA ACCTGAAGAA AAGAAAGAAG TTTTCAGACC TCTCAAGCCT
    GCCGGCGAAG TGGATTTGAC TGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTGCGA
    CCACCACACA AAGTGACAGA CTACTCCTCA TCCAGTGAGG AGTCCGGGAC AACAGATGAG
    GAAGACGATG ATGTAGAACA AGAAGGGGCT GAGGAAGCCA CCTCTGGACC AGAGGACACC
    AGAGCAGCGT CGTCCCTGAA TTTGAGCAAT GGTGAAACAG AATCCGTGAA AACCATGATT
    GTTCATGACG ACGTAGAAAG TGAACCAGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC
    GTCCGCCAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGTGGGAC AACAGTGACT
    TCTGTGGTGG GATTTTCCTG TGATGGAATG AGACCAGAAG CCATAAGGCA AGATCCTACC
    CGGAAGGGCT CAGTGGTCAA TGTGAATCCC ACCAACACTA GGCCACAGAG TGATACCCCG
    GAGATTCGTA AATATAAGAA GAGATTTAAC TCCGAGATTC TGTGTGCTGC CTTATGGGGA
    GTGAATTTGC TAGTGGGTAC AGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG
    AAGGTATATC CCCTGATCAA CCGAAGACGA TTTCAGCAAA TGGATGTCCT TGAAGGCTTG
    AATGTCTTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG
    TTAAGAAATA AAATACTTCA CAATGATCCA GAAGTTGAGA AGAAGCAGGG ATGGACGACT
    GTGGGAGATT TGGAAGGATG TGTACACTAT AAAGTTGTAA AATATGAAAG AATCAAATTT
    CTGGTAATTG CTTTGAAGAG TTCTGTGGAG GTCTATGCGT GGGCACCCAA GCCATATCAC
    AAATTTATGG CCTTTAAGTC ATTTGGGGAA TTAGTACATA AGCCATTGCT GGTGGATCTC
    ACTGTGGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT
    GTTGATGTGG ATTCTGGATC AGTCTATGAC ATTTATCTAC CAACACACGT AAGAAAGAAC
    CCACACTCTA TGATCCAGTG TAGCATCAAA CCCCATGCAA TCATCATCCT CCCCAACACA
    GATGGCATGG AGCTTCTGGT GTGCTATGAA GATGAAGGGG TTTATGTGAA TACTTATGGA
    AGAATCACCA AGGATGTGGT TCTGCAGTGG GGAGAGATGC CAACATCTGT AGCATATATT
    CGATCCAACC AGACGATGGG CTGGGGAGAA AAGGCCATAG AGATCCGATC TGTGGAAACT
    GGTCATTTGG ATGGTGTGTT TATGCACAAA AGGGCTCAAA GACTAAAATT CCTATGTGAA
    CGCAATGACA AGGTCTTCTT TGCCTCTGTT CGGTCTGGTG GCAGCAGCCA GGTTTATTTC
    ATGACGTTAG GCAGGACTTC TCTTCTGAGC TGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280274.1
    CDS175..3990
    Translation

    Target ORF information:

    RefSeq Version XM_022424566.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424566.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGGTACAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCTCCCAAAT TTGCACACCA CCATCTTCGC
    TCTCAGGACC CATGTCCACC TTCCCGCAGT GAGGTGCTAA GTCAGAGTTC TGACTCTAAG
    TCGGAGGTAC CTGACCCCAC CCAAAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT
    CCAAGGGTTC CTGTGAGGAC AACGTCTCGT TCCCCTGTTC TGTCCCGTCG GGATTCCCCA
    CTGCAAGGCA GTGGACAGCA AAATAGTCAA GCAGGTCAAA GAAACTCCAC TAGCAGTATT
    GAGCCCCGGC TGCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC
    TCCTCCGGAT CCAGCAACTC CGGATCCCAG CCTGGGTCCC ACCCTGGGTC CCAGAGTGGC
    TCTGGAGAGC GCTTCAGAGT GAGATCATCA TCCAAATCTG AAGGCTCTCC TTCTCAGCGC
    CTAGAAAATG CAGTGAAAAA ACCTGAAGAA AAGAAAGAAG TTTTCAGACC TCTCAAGCCT
    GCCGGCGAAG TGGATTTGAC TGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTGCGA
    CCACCACACA AAGTGACAGA CTACTCCTCA TCCAGTGAGG AGTCCGGGAC AACAGATGAG
    GAAGACGATG ATGTAGAACA AGAAGGGGCT GAGGAAGCCA CCTCTGGACC AGAGGACACC
    AGAGCAGCGT CGTCCCTGAA TTTGAGCAAT GGTGAAACAG AATCCGTGAA AACCATGATT
    GTTCATGACG ACGTAGAAAG TGAACCAGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC
    GTCCGCCAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGTGGGAC AACAGTGACT
    TCTGTGGTGG GATTTTCCTG TGATGGAATG AGACCAGAAG CCATAAGGCA AGATCCTACC
    CGGAAGGGCT CAGTGGTCAA TGTGAATCCC ACCAACACTA GGCCACAGAG TGATACCCCG
    GAGATTCGTA AATATAAGAA GAGATTTAAC TCCGAGATTC TGTGTGCTGC CTTATGGGGA
    GTGAATTTGC TAGTGGGTAC AGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG
    AAGGTATATC CCCTGATCAA CCGAAGACGA TTTCAGCAAA TGGATGTCCT TGAAGGCTTG
    AATGTCTTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG
    TTAAGAAATA AAATACTTCA CAATGATCCA GAAGTTGAGA AGAAGCAGGG ATGGACGACT
    GTGGGAGATT TGGAAGGATG TGTACACTAT AAAGTTGTAA AATATGAAAG AATCAAATTT
    CTGGTAATTG CTTTGAAGAG TTCTGTGGAG GTCTATGCGT GGGCACCCAA GCCATATCAC
    AAATTTATGG CCTTTAAGTC ATTTGGGGAA TTAGTACATA AGCCATTGCT GGTGGATCTC
    ACTGTGGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT
    GTTGATGTGG ATTCTGGATC AGTCTATGAC ATTTATCTAC CAACACACGT AAGAAAGAAC
    CCACACTCTA TGATCCAGTG TAGCATCAAA CCCCATGCAA TCATCATCCT CCCCAACACA
    GATGGCATGG AGCTTCTGGT GTGCTATGAA GATGAAGGGG TTTATGTGAA TACTTATGGA
    AGAATCACCA AGGATGTGGT TCTGCAGTGG GGAGAGATGC CAACATCTGT AGCATATATT
    CGATCCAACC AGACGATGGG CTGGGGAGAA AAGGCCATAG AGATCCGATC TGTGGAAACT
    GGTCATTTGG ATGGTGTGTT TATGCACAAA AGGGCTCAAA GACTAAAATT CCTATGTGAA
    CGCAATGACA AGGTCTTCTT TGCCTCTGTT CGGTCTGGTG GCAGCAGCCA GGTTTATTTC
    ATGACGTTAG GCAGGACTTC TCTTCTGAGC TGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291550
    Clone ID Related Accession (Same CDS sequence) XM_022424567.1
    Accession Version XM_022424567.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGGTACAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCTCCCAAAT TTGCACACCA CCATCTTCGC
    TCTCAGGACC CATGTCCACC TTCCCGCAGT GAGGTGCTAA GTCAGAGTTC TGACTCTAAG
    TCGGAGGTAC CTGACCCCAC CCAAAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT
    CCAAGGGTTC CTGTGAGGAC AACGTCTCGT TCCCCTGTTC TGTCCCGTCG GGATTCCCCA
    CTGCAAGGCA GTGGACAGCA AAATAGTCAA GCAGGTCAAA GAAACTCCAC TAGCAGTATT
    GAGCCCCGGC TGCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC
    TCCTCCGGAT CCAGCAACTC CGGATCCCAG CCTGGGTCCC ACCCTGGGTC CCAGAGTGGC
    TCTGGAGAGC GCTTCAGAGT GAGATCATCA TCCAAATCTG AAGGCTCTCC TTCTCAGCGC
    CTAGAAAATG CAGTGAAAAA ACCTGAAGAA AAGAAAGAAG TTTTCAGACC TCTCAAGCCT
    GCCGGCGAAG TGGATTTGAC TGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTGCGA
    CCACCACACA AAGTGACAGA CTACTCCTCA TCCAGTGAGG AGTCCGGGAC AACAGATGAG
    GAAGACGATG ATGTAGAACA AGAAGGGGCT GAGGAAGCCA CCTCTGGACC AGAGGACACC
    AGAGCAGCGT CGTCCCTGAA TTTGAGCAAT GGTGAAACAG AATCCGTGAA AACCATGATT
    GTTCATGACG ACGTAGAAAG TGAACCAGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC
    GTCCGCCAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGTGGGAC AACAGTGACT
    TCTGTGGTGG GATTTTCCTG TGATGGAATG AGACCAGAAG CCATAAGGCA AGATCCTACC
    CGGAAGGGCT CAGTGGTCAA TGTGAATCCC ACCAACACTA GGCCACAGAG TGATACCCCG
    GAGATTCGTA AATATAAGAA GAGATTTAAC TCCGAGATTC TGTGTGCTGC CTTATGGGGA
    GTGAATTTGC TAGTGGGTAC AGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG
    AAGGTATATC CCCTGATCAA CCGAAGACGA TTTCAGCAAA TGGATGTCCT TGAAGGCTTG
    AATGTCTTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG
    TTAAGAAATA AAATACTTCA CAATGATCCA GAAGTTGAGA AGAAGCAGGG ATGGACGACT
    GTGGGAGATT TGGAAGGATG TGTACACTAT AAAGTTGTAA AATATGAAAG AATCAAATTT
    CTGGTAATTG CTTTGAAGAG TTCTGTGGAG GTCTATGCGT GGGCACCCAA GCCATATCAC
    AAATTTATGG CCTTTAAGTC ATTTGGGGAA TTAGTACATA AGCCATTGCT GGTGGATCTC
    ACTGTGGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT
    GTTGATGTGG ATTCTGGATC AGTCTATGAC ATTTATCTAC CAACACACAT CCAGTGTAGC
    ATCAAACCCC ATGCAATCAT CATCCTCCCC AACACAGATG GCATGGAGCT TCTGGTGTGC
    TATGAAGATG AAGGGGTTTA TGTGAATACT TATGGAAGAA TCACCAAGGA TGTGGTTCTG
    CAGTGGGGAG AGATGCCAAC ATCTGTAGCA TATATTCGAT CCAACCAGAC GATGGGCTGG
    GGAGAAAAGG CCATAGAGAT CCGATCTGTG GAAACTGGTC ATTTGGATGG TGTGTTTATG
    CACAAAAGGG CTCAAAGACT AAAATTCCTA TGTGAACGCA ATGACAAGGT CTTCTTTGCC
    TCTGTTCGGT CTGGTGGCAG CAGCCAGGTT TATTTCATGA CGTTAGGCAG GACTTCTCTT
    CTGAGCTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280275.1
    CDS175..3966
    Translation

    Target ORF information:

    RefSeq Version XM_022424567.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424567.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGGTACAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCTCCCAAAT TTGCACACCA CCATCTTCGC
    TCTCAGGACC CATGTCCACC TTCCCGCAGT GAGGTGCTAA GTCAGAGTTC TGACTCTAAG
    TCGGAGGTAC CTGACCCCAC CCAAAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT
    CCAAGGGTTC CTGTGAGGAC AACGTCTCGT TCCCCTGTTC TGTCCCGTCG GGATTCCCCA
    CTGCAAGGCA GTGGACAGCA AAATAGTCAA GCAGGTCAAA GAAACTCCAC TAGCAGTATT
    GAGCCCCGGC TGCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC
    TCCTCCGGAT CCAGCAACTC CGGATCCCAG CCTGGGTCCC ACCCTGGGTC CCAGAGTGGC
    TCTGGAGAGC GCTTCAGAGT GAGATCATCA TCCAAATCTG AAGGCTCTCC TTCTCAGCGC
    CTAGAAAATG CAGTGAAAAA ACCTGAAGAA AAGAAAGAAG TTTTCAGACC TCTCAAGCCT
    GCCGGCGAAG TGGATTTGAC TGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTGCGA
    CCACCACACA AAGTGACAGA CTACTCCTCA TCCAGTGAGG AGTCCGGGAC AACAGATGAG
    GAAGACGATG ATGTAGAACA AGAAGGGGCT GAGGAAGCCA CCTCTGGACC AGAGGACACC
    AGAGCAGCGT CGTCCCTGAA TTTGAGCAAT GGTGAAACAG AATCCGTGAA AACCATGATT
    GTTCATGACG ACGTAGAAAG TGAACCAGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC
    GTCCGCCAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGTGGGAC AACAGTGACT
    TCTGTGGTGG GATTTTCCTG TGATGGAATG AGACCAGAAG CCATAAGGCA AGATCCTACC
    CGGAAGGGCT CAGTGGTCAA TGTGAATCCC ACCAACACTA GGCCACAGAG TGATACCCCG
    GAGATTCGTA AATATAAGAA GAGATTTAAC TCCGAGATTC TGTGTGCTGC CTTATGGGGA
    GTGAATTTGC TAGTGGGTAC AGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG
    AAGGTATATC CCCTGATCAA CCGAAGACGA TTTCAGCAAA TGGATGTCCT TGAAGGCTTG
    AATGTCTTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG
    TTAAGAAATA AAATACTTCA CAATGATCCA GAAGTTGAGA AGAAGCAGGG ATGGACGACT
    GTGGGAGATT TGGAAGGATG TGTACACTAT AAAGTTGTAA AATATGAAAG AATCAAATTT
    CTGGTAATTG CTTTGAAGAG TTCTGTGGAG GTCTATGCGT GGGCACCCAA GCCATATCAC
    AAATTTATGG CCTTTAAGTC ATTTGGGGAA TTAGTACATA AGCCATTGCT GGTGGATCTC
    ACTGTGGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT
    GTTGATGTGG ATTCTGGATC AGTCTATGAC ATTTATCTAC CAACACACAT CCAGTGTAGC
    ATCAAACCCC ATGCAATCAT CATCCTCCCC AACACAGATG GCATGGAGCT TCTGGTGTGC
    TATGAAGATG AAGGGGTTTA TGTGAATACT TATGGAAGAA TCACCAAGGA TGTGGTTCTG
    CAGTGGGGAG AGATGCCAAC ATCTGTAGCA TATATTCGAT CCAACCAGAC GATGGGCTGG
    GGAGAAAAGG CCATAGAGAT CCGATCTGTG GAAACTGGTC ATTTGGATGG TGTGTTTATG
    CACAAAAGGG CTCAAAGACT AAAATTCCTA TGTGAACGCA ATGACAAGGT CTTCTTTGCC
    TCTGTTCGGT CTGGTGGCAG CAGCCAGGTT TATTTCATGA CGTTAGGCAG GACTTCTCTT
    CTGAGCTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291551
    Clone ID Related Accession (Same CDS sequence) XM_022424568.1
    Accession Version XM_022424568.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGGTTCCTGT GAGGACAACG TCTCGTTCCC CTGTTCTGTC CCGTCGGGAT
    TCCCCACTGC AAGGCAGTGG ACAGCAAAAT AGTCAAGCAG GTCAAAGAAA CTCCACTAGC
    AGTATTGAGC CCCGGCTGCT GTGGGAGAGA GTGGAGAAGC TGGTGCCCAG GCCTGGCAGT
    GGCAGCTCCT CCGGATCCAG CAACTCCGGA TCCCAGCCTG GGTCCCACCC TGGGTCCCAG
    AGTGGCTCTG GAGAGCGCTT CAGAGTGAGA TCATCATCCA AATCTGAAGG CTCTCCTTCT
    CAGCGCCTAG AAAATGCAGT GAAAAAACCT GAAGAAAAGA AAGAAGTTTT CAGACCTCTC
    AAGCCTGCCG GCGAAGTGGA TTTGACTGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTGCGACCAC CACACAAAGT GACAGACTAC TCCTCATCCA GTGAGGAGTC CGGGACAACA
    GATGAGGAAG ACGATGATGT AGAACAAGAA GGGGCTGAGG AAGCCACCTC TGGACCAGAG
    GACACCAGAG CAGCGTCGTC CCTGAATTTG AGCAATGGTG AAACAGAATC CGTGAAAACC
    ATGATTGTTC ATGACGACGT AGAAAGTGAA CCAGCCATGA CCCCATCCAA GGAGGGCACT
    CTAATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGCGCCA GCCATCATGA GAGCAATGGC
    TTTGCCGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT
    TCCTCCACCT CCTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA
    CCCCAGGACA AGCTCACTAC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT
    AGTGGGACAA CAGTGACTTC TGTGGTGGGA TTTTCCTGTG ATGGAATGAG ACCAGAAGCC
    ATAAGGCAAG ATCCTACCCG GAAGGGCTCA GTGGTCAATG TGAATCCCAC CAACACTAGG
    CCACAGAGTG ATACCCCGGA GATTCGTAAA TATAAGAAGA GATTTAACTC CGAGATTCTG
    TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG
    GACAGAAGTG GCCAAGGGAA GGTATATCCC CTGATCAACC GAAGACGATT TCAGCAAATG
    GATGTCCTTG AAGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT
    GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG
    AAGCAGGGAT GGACGACTGT GGGAGATTTG GAAGGATGTG TACACTATAA AGTTGTAAAA
    TATGAAAGAA TCAAATTTCT GGTAATTGCT TTGAAGAGTT CTGTGGAGGT CTATGCGTGG
    GCACCCAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGGGAATT AGTACATAAG
    CCATTGCTGG TGGATCTCAC TGTGGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC
    TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCTGGATCAG TCTATGACAT TTATCTACCA
    ACACACGTAA GAAAGAACCC ACACTCTATG ATCCAGTGTA GCATCAAACC CCATGCAATC
    ATCATCCTCC CCAACACAGA TGGCATGGAG CTTCTGGTGT GCTATGAAGA TGAAGGGGTT
    TATGTGAATA CTTATGGAAG AATCACCAAG GATGTGGTTC TGCAGTGGGG AGAGATGCCA
    ACATCTGTAG CATATATTCG ATCCAACCAG ACGATGGGCT GGGGAGAAAA GGCCATAGAG
    ATCCGATCTG TGGAAACTGG TCATTTGGAT GGTGTGTTTA TGCACAAAAG GGCTCAAAGA
    CTAAAATTCC TATGTGAACG CAATGACAAG GTCTTCTTTG CCTCTGTTCG GTCTGGTGGC
    AGCAGCCAGG TTTATTTCAT GACGTTAGGC AGGACTTCTC TTCTGAGCTG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280276.1
    CDS175..3948
    Translation

    Target ORF information:

    RefSeq Version XM_022424568.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424568.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGGTTCCTGT GAGGACAACG TCTCGTTCCC CTGTTCTGTC CCGTCGGGAT
    TCCCCACTGC AAGGCAGTGG ACAGCAAAAT AGTCAAGCAG GTCAAAGAAA CTCCACTAGC
    AGTATTGAGC CCCGGCTGCT GTGGGAGAGA GTGGAGAAGC TGGTGCCCAG GCCTGGCAGT
    GGCAGCTCCT CCGGATCCAG CAACTCCGGA TCCCAGCCTG GGTCCCACCC TGGGTCCCAG
    AGTGGCTCTG GAGAGCGCTT CAGAGTGAGA TCATCATCCA AATCTGAAGG CTCTCCTTCT
    CAGCGCCTAG AAAATGCAGT GAAAAAACCT GAAGAAAAGA AAGAAGTTTT CAGACCTCTC
    AAGCCTGCCG GCGAAGTGGA TTTGACTGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTGCGACCAC CACACAAAGT GACAGACTAC TCCTCATCCA GTGAGGAGTC CGGGACAACA
    GATGAGGAAG ACGATGATGT AGAACAAGAA GGGGCTGAGG AAGCCACCTC TGGACCAGAG
    GACACCAGAG CAGCGTCGTC CCTGAATTTG AGCAATGGTG AAACAGAATC CGTGAAAACC
    ATGATTGTTC ATGACGACGT AGAAAGTGAA CCAGCCATGA CCCCATCCAA GGAGGGCACT
    CTAATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGCGCCA GCCATCATGA GAGCAATGGC
    TTTGCCGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT
    TCCTCCACCT CCTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA
    CCCCAGGACA AGCTCACTAC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT
    AGTGGGACAA CAGTGACTTC TGTGGTGGGA TTTTCCTGTG ATGGAATGAG ACCAGAAGCC
    ATAAGGCAAG ATCCTACCCG GAAGGGCTCA GTGGTCAATG TGAATCCCAC CAACACTAGG
    CCACAGAGTG ATACCCCGGA GATTCGTAAA TATAAGAAGA GATTTAACTC CGAGATTCTG
    TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG
    GACAGAAGTG GCCAAGGGAA GGTATATCCC CTGATCAACC GAAGACGATT TCAGCAAATG
    GATGTCCTTG AAGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT
    GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG
    AAGCAGGGAT GGACGACTGT GGGAGATTTG GAAGGATGTG TACACTATAA AGTTGTAAAA
    TATGAAAGAA TCAAATTTCT GGTAATTGCT TTGAAGAGTT CTGTGGAGGT CTATGCGTGG
    GCACCCAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGGGAATT AGTACATAAG
    CCATTGCTGG TGGATCTCAC TGTGGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC
    TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCTGGATCAG TCTATGACAT TTATCTACCA
    ACACACGTAA GAAAGAACCC ACACTCTATG ATCCAGTGTA GCATCAAACC CCATGCAATC
    ATCATCCTCC CCAACACAGA TGGCATGGAG CTTCTGGTGT GCTATGAAGA TGAAGGGGTT
    TATGTGAATA CTTATGGAAG AATCACCAAG GATGTGGTTC TGCAGTGGGG AGAGATGCCA
    ACATCTGTAG CATATATTCG ATCCAACCAG ACGATGGGCT GGGGAGAAAA GGCCATAGAG
    ATCCGATCTG TGGAAACTGG TCATTTGGAT GGTGTGTTTA TGCACAAAAG GGCTCAAAGA
    CTAAAATTCC TATGTGAACG CAATGACAAG GTCTTCTTTG CCTCTGTTCG GTCTGGTGGC
    AGCAGCCAGG TTTATTTCAT GACGTTAGGC AGGACTTCTC TTCTGAGCTG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291552
    Clone ID Related Accession (Same CDS sequence) XM_022424569.1
    Accession Version XM_022424569.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3771bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGGTTCCTGT GAGGACAACG TCTCGTTCCC CTGTTCTGTC CCGTCGGGAT
    TCCCCACTGC AAGGCAGTGG ACAGCAAAAT AGTCAAGCAG GTCAAAGAAA CTCCACTAGT
    ATTGAGCCCC GGCTGCTGTG GGAGAGAGTG GAGAAGCTGG TGCCCAGGCC TGGCAGTGGC
    AGCTCCTCCG GATCCAGCAA CTCCGGATCC CAGCCTGGGT CCCACCCTGG GTCCCAGAGT
    GGCTCTGGAG AGCGCTTCAG AGTGAGATCA TCATCCAAAT CTGAAGGCTC TCCTTCTCAG
    CGCCTAGAAA ATGCAGTGAA AAAACCTGAA GAAAAGAAAG AAGTTTTCAG ACCTCTCAAG
    CCTGCCGGCG AAGTGGATTT GACTGCACTG GCCAAAGAGC TTCGAGCAGT GGAAGATGTG
    CGACCACCAC ACAAAGTGAC AGACTACTCC TCATCCAGTG AGGAGTCCGG GACAACAGAT
    GAGGAAGACG ATGATGTAGA ACAAGAAGGG GCTGAGGAAG CCACCTCTGG ACCAGAGGAC
    ACCAGAGCAG CGTCGTCCCT GAATTTGAGC AATGGTGAAA CAGAATCCGT GAAAACCATG
    ATTGTTCATG ACGACGTAGA AAGTGAACCA GCCATGACCC CATCCAAGGA GGGCACTCTA
    ATCGTCCGCC AGAGTACAGT TGACCAAAAG CGCGCCAGCC ATCATGAGAG CAATGGCTTT
    GCCGGTCGCA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACTTCC
    TCCACCTCCT CCTCCCCATC CTCCAGCCAG CCGACACCCA CCATGTCCCC ACAGACACCC
    CAGGACAAGC TCACTACTAA TGAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGATTAC TACAGATTTC TCCATCTAGT
    GGGACAACAG TGACTTCTGT GGTGGGATTT TCCTGTGATG GAATGAGACC AGAAGCCATA
    AGGCAAGATC CTACCCGGAA GGGCTCAGTG GTCAATGTGA ATCCCACCAA CACTAGGCCA
    CAGAGTGATA CCCCGGAGAT TCGTAAATAT AAGAAGAGAT TTAACTCCGA GATTCTGTGT
    GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG GGTACAGAGA GTGGCCTGAT GCTGCTGGAC
    AGAAGTGGCC AAGGGAAGGT ATATCCCCTG ATCAACCGAA GACGATTTCA GCAAATGGAT
    GTCCTTGAAG GCTTGAATGT CTTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGTGTC
    TACTATTTGT CCTGGTTAAG AAATAAAATA CTTCACAATG ATCCAGAAGT TGAGAAGAAG
    CAGGGATGGA CGACTGTGGG AGATTTGGAA GGATGTGTAC ACTATAAAGT TGTAAAATAT
    GAAAGAATCA AATTTCTGGT AATTGCTTTG AAGAGTTCTG TGGAGGTCTA TGCGTGGGCA
    CCCAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GGGAATTAGT ACATAAGCCA
    TTGCTGGTGG ATCTCACTGT GGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT
    GCTGGATTCC ATGCTGTTGA TGTGGATTCT GGATCAGTCT ATGACATTTA TCTACCAACA
    CACGTAAGAA AGAACCCACA CTCTATGATC CAGTGTAGCA TCAAACCCCA TGCAATCATC
    ATCCTCCCCA ACACAGATGG CATGGAGCTT CTGGTGTGCT ATGAAGATGA AGGGGTTTAT
    GTGAATACTT ATGGAAGAAT CACCAAGGAT GTGGTTCTGC AGTGGGGAGA GATGCCAACA
    TCTGTAGCAT ATATTCGATC CAACCAGACG ATGGGCTGGG GAGAAAAGGC CATAGAGATC
    CGATCTGTGG AAACTGGTCA TTTGGATGGT GTGTTTATGC ACAAAAGGGC TCAAAGACTA
    AAATTCCTAT GTGAACGCAA TGACAAGGTC TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC
    AGCCAGGTTT ATTTCATGAC GTTAGGCAGG ACTTCTCTTC TGAGCTGGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280277.1
    CDS175..3945
    Translation

    Target ORF information:

    RefSeq Version XM_022424569.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424569.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGGTTCCTGT GAGGACAACG TCTCGTTCCC CTGTTCTGTC CCGTCGGGAT
    TCCCCACTGC AAGGCAGTGG ACAGCAAAAT AGTCAAGCAG GTCAAAGAAA CTCCACTAGT
    ATTGAGCCCC GGCTGCTGTG GGAGAGAGTG GAGAAGCTGG TGCCCAGGCC TGGCAGTGGC
    AGCTCCTCCG GATCCAGCAA CTCCGGATCC CAGCCTGGGT CCCACCCTGG GTCCCAGAGT
    GGCTCTGGAG AGCGCTTCAG AGTGAGATCA TCATCCAAAT CTGAAGGCTC TCCTTCTCAG
    CGCCTAGAAA ATGCAGTGAA AAAACCTGAA GAAAAGAAAG AAGTTTTCAG ACCTCTCAAG
    CCTGCCGGCG AAGTGGATTT GACTGCACTG GCCAAAGAGC TTCGAGCAGT GGAAGATGTG
    CGACCACCAC ACAAAGTGAC AGACTACTCC TCATCCAGTG AGGAGTCCGG GACAACAGAT
    GAGGAAGACG ATGATGTAGA ACAAGAAGGG GCTGAGGAAG CCACCTCTGG ACCAGAGGAC
    ACCAGAGCAG CGTCGTCCCT GAATTTGAGC AATGGTGAAA CAGAATCCGT GAAAACCATG
    ATTGTTCATG ACGACGTAGA AAGTGAACCA GCCATGACCC CATCCAAGGA GGGCACTCTA
    ATCGTCCGCC AGAGTACAGT TGACCAAAAG CGCGCCAGCC ATCATGAGAG CAATGGCTTT
    GCCGGTCGCA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACTTCC
    TCCACCTCCT CCTCCCCATC CTCCAGCCAG CCGACACCCA CCATGTCCCC ACAGACACCC
    CAGGACAAGC TCACTACTAA TGAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGATTAC TACAGATTTC TCCATCTAGT
    GGGACAACAG TGACTTCTGT GGTGGGATTT TCCTGTGATG GAATGAGACC AGAAGCCATA
    AGGCAAGATC CTACCCGGAA GGGCTCAGTG GTCAATGTGA ATCCCACCAA CACTAGGCCA
    CAGAGTGATA CCCCGGAGAT TCGTAAATAT AAGAAGAGAT TTAACTCCGA GATTCTGTGT
    GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG GGTACAGAGA GTGGCCTGAT GCTGCTGGAC
    AGAAGTGGCC AAGGGAAGGT ATATCCCCTG ATCAACCGAA GACGATTTCA GCAAATGGAT
    GTCCTTGAAG GCTTGAATGT CTTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGTGTC
    TACTATTTGT CCTGGTTAAG AAATAAAATA CTTCACAATG ATCCAGAAGT TGAGAAGAAG
    CAGGGATGGA CGACTGTGGG AGATTTGGAA GGATGTGTAC ACTATAAAGT TGTAAAATAT
    GAAAGAATCA AATTTCTGGT AATTGCTTTG AAGAGTTCTG TGGAGGTCTA TGCGTGGGCA
    CCCAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GGGAATTAGT ACATAAGCCA
    TTGCTGGTGG ATCTCACTGT GGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT
    GCTGGATTCC ATGCTGTTGA TGTGGATTCT GGATCAGTCT ATGACATTTA TCTACCAACA
    CACGTAAGAA AGAACCCACA CTCTATGATC CAGTGTAGCA TCAAACCCCA TGCAATCATC
    ATCCTCCCCA ACACAGATGG CATGGAGCTT CTGGTGTGCT ATGAAGATGA AGGGGTTTAT
    GTGAATACTT ATGGAAGAAT CACCAAGGAT GTGGTTCTGC AGTGGGGAGA GATGCCAACA
    TCTGTAGCAT ATATTCGATC CAACCAGACG ATGGGCTGGG GAGAAAAGGC CATAGAGATC
    CGATCTGTGG AAACTGGTCA TTTGGATGGT GTGTTTATGC ACAAAAGGGC TCAAAGACTA
    AAATTCCTAT GTGAACGCAA TGACAAGGTC TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC
    AGCCAGGTTT ATTTCATGAC GTTAGGCAGG ACTTCTCTTC TGAGCTGGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291553
    Clone ID Related Accession (Same CDS sequence) XM_022424570.1
    Accession Version XM_022424570.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGCAT
    GACCACAGGA GGCCGCACCC GCAGCAGCCG CCGCCACCGC AGCAGGAAAG GAGCAAGCCA
    AGCTATCACG CTCCGGAGCC TAAGCCCCAC TATGAGCCTG CTGACAGAGC TCGAGAGGTA
    CAGTGGTCCC ACCTGGCATC TCTCAAGAAC AATGTTTCCC CTGTCTCGCG ATCCCATTCC
    TTCAGTGACC CTTCTCCTCC CAAATTTGCA CACCACCATC TTCGCTCTCA GGACCCATGT
    CCACCTTCCC GCAGTGAGGT GCTAAGTCAG AGTTCTGACT CTAAGTCGGA GGTACCTGAC
    CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG
    AGGACAACGT CTCGTTCCCC TGTTCTGTCC CGTCGGGATT CCCCACTGCA AGGCAGTGGA
    CAGCAAAATA GTCAAGCAGG TCAAAGAAAC TCCACTAGCA GTATTGAGCC CCGGCTGCTG
    TGGGAGAGAG TGGAGAAGCT GGTGCCCAGG CCTGGCAGTG GCAGCTCCTC CGGATCCAGC
    AACTCCGGAT CCCAGCCTGG GTCCCACCCT GGGTCCCAGA GTGGCTCTGG AGAGCGCTTC
    AGAGTGAGAT CATCATCCAA ATCTGAAGGC TCTCCTTCTC AGCGCCTAGA AAATGCAGTG
    AAAAAACCTG AAGAAAAGAA AGAAGTTTTC AGACCTCTCA AGCCTGCCGG CGAAGTGGAT
    TTGACTGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGACCACC ACACAAAGTG
    ACAGACTACT CCTCATCCAG TGAGGAGTCC GGGACAACAG ATGAGGAAGA CGATGATGTA
    GAACAAGAAG GGGCTGAGGA AGCCACCTCT GGACCAGAGG ACACCAGAGC AGCGTCGTCC
    CTGAATTTGA GCAATGGTGA AACAGAATCC GTGAAAACCA TGATTGTTCA TGACGACGTA
    GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGAGTACA
    GTTGACCAAA AGCGCGCCAG CCATCATGAG AGCAATGGCT TTGCCGGTCG CATTCACCTC
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCCTCCCCA
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTACT
    AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGGACAAC AGTGACTTCT
    GTGGTGGGAT TTTCCTGTGA TGGAATGAGA CCAGAAGCCA TAAGGCAAGA TCCTACCCGG
    AAGGGCTCAG TGGTCAATGT GAATCCCACC AACACTAGGC CACAGAGTGA TACCCCGGAG
    ATTCGTAAAT ATAAGAAGAG ATTTAACTCC GAGATTCTGT GTGCTGCCTT ATGGGGAGTG
    AATTTGCTAG TGGGTACAGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG
    GTATATCCCC TGATCAACCG AAGACGATTT CAGCAAATGG ATGTCCTTGA AGGCTTGAAT
    GTCTTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA
    AGAAATAAAA TACTTCACAA TGATCCAGAA GTTGAGAAGA AGCAGGGATG GACGACTGTG
    GGAGATTTGG AAGGATGTGT ACACTATAAA GTTGTAAAAT ATGAAAGAAT CAAATTTCTG
    GTAATTGCTT TGAAGAGTTC TGTGGAGGTC TATGCGTGGG CACCCAAGCC ATATCACAAA
    TTTATGGCCT TTAAGTCATT TGGGGAATTA GTACATAAGC CATTGCTGGT GGATCTCACT
    GTGGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT
    GATGTGGATT CTGGATCAGT CTATGACATT TATCTACCAA CACACGTAAG AAAGAACCCA
    CACTCTATGA TCCAGTGTAG CATCAAACCC CATGCAATCA TCATCCTCCC CAACACAGAT
    GGCATGGAGC TTCTGGTGTG CTATGAAGAT GAAGGGGTTT ATGTGAATAC TTATGGAAGA
    ATCACCAAGG ATGTGGTTCT GCAGTGGGGA GAGATGCCAA CATCTGTAGC ATATATTCGA
    TCCAACCAGA CGATGGGCTG GGGAGAAAAG GCCATAGAGA TCCGATCTGT GGAAACTGGT
    CATTTGGATG GTGTGTTTAT GCACAAAAGG GCTCAAAGAC TAAAATTCCT ATGTGAACGC
    AATGACAAGG TCTTCTTTGC CTCTGTTCGG TCTGGTGGCA GCAGCCAGGT TTATTTCATG
    ACGTTAGGCA GGACTTCTCT TCTGAGCTGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280278.1
    CDS175..3927
    Translation

    Target ORF information:

    RefSeq Version XM_022424570.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424570.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGCAT
    GACCACAGGA GGCCGCACCC GCAGCAGCCG CCGCCACCGC AGCAGGAAAG GAGCAAGCCA
    AGCTATCACG CTCCGGAGCC TAAGCCCCAC TATGAGCCTG CTGACAGAGC TCGAGAGGTA
    CAGTGGTCCC ACCTGGCATC TCTCAAGAAC AATGTTTCCC CTGTCTCGCG ATCCCATTCC
    TTCAGTGACC CTTCTCCTCC CAAATTTGCA CACCACCATC TTCGCTCTCA GGACCCATGT
    CCACCTTCCC GCAGTGAGGT GCTAAGTCAG AGTTCTGACT CTAAGTCGGA GGTACCTGAC
    CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG
    AGGACAACGT CTCGTTCCCC TGTTCTGTCC CGTCGGGATT CCCCACTGCA AGGCAGTGGA
    CAGCAAAATA GTCAAGCAGG TCAAAGAAAC TCCACTAGCA GTATTGAGCC CCGGCTGCTG
    TGGGAGAGAG TGGAGAAGCT GGTGCCCAGG CCTGGCAGTG GCAGCTCCTC CGGATCCAGC
    AACTCCGGAT CCCAGCCTGG GTCCCACCCT GGGTCCCAGA GTGGCTCTGG AGAGCGCTTC
    AGAGTGAGAT CATCATCCAA ATCTGAAGGC TCTCCTTCTC AGCGCCTAGA AAATGCAGTG
    AAAAAACCTG AAGAAAAGAA AGAAGTTTTC AGACCTCTCA AGCCTGCCGG CGAAGTGGAT
    TTGACTGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGACCACC ACACAAAGTG
    ACAGACTACT CCTCATCCAG TGAGGAGTCC GGGACAACAG ATGAGGAAGA CGATGATGTA
    GAACAAGAAG GGGCTGAGGA AGCCACCTCT GGACCAGAGG ACACCAGAGC AGCGTCGTCC
    CTGAATTTGA GCAATGGTGA AACAGAATCC GTGAAAACCA TGATTGTTCA TGACGACGTA
    GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGAGTACA
    GTTGACCAAA AGCGCGCCAG CCATCATGAG AGCAATGGCT TTGCCGGTCG CATTCACCTC
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCCTCCCCA
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTACT
    AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGGACAAC AGTGACTTCT
    GTGGTGGGAT TTTCCTGTGA TGGAATGAGA CCAGAAGCCA TAAGGCAAGA TCCTACCCGG
    AAGGGCTCAG TGGTCAATGT GAATCCCACC AACACTAGGC CACAGAGTGA TACCCCGGAG
    ATTCGTAAAT ATAAGAAGAG ATTTAACTCC GAGATTCTGT GTGCTGCCTT ATGGGGAGTG
    AATTTGCTAG TGGGTACAGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG
    GTATATCCCC TGATCAACCG AAGACGATTT CAGCAAATGG ATGTCCTTGA AGGCTTGAAT
    GTCTTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA
    AGAAATAAAA TACTTCACAA TGATCCAGAA GTTGAGAAGA AGCAGGGATG GACGACTGTG
    GGAGATTTGG AAGGATGTGT ACACTATAAA GTTGTAAAAT ATGAAAGAAT CAAATTTCTG
    GTAATTGCTT TGAAGAGTTC TGTGGAGGTC TATGCGTGGG CACCCAAGCC ATATCACAAA
    TTTATGGCCT TTAAGTCATT TGGGGAATTA GTACATAAGC CATTGCTGGT GGATCTCACT
    GTGGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT
    GATGTGGATT CTGGATCAGT CTATGACATT TATCTACCAA CACACGTAAG AAAGAACCCA
    CACTCTATGA TCCAGTGTAG CATCAAACCC CATGCAATCA TCATCCTCCC CAACACAGAT
    GGCATGGAGC TTCTGGTGTG CTATGAAGAT GAAGGGGTTT ATGTGAATAC TTATGGAAGA
    ATCACCAAGG ATGTGGTTCT GCAGTGGGGA GAGATGCCAA CATCTGTAGC ATATATTCGA
    TCCAACCAGA CGATGGGCTG GGGAGAAAAG GCCATAGAGA TCCGATCTGT GGAAACTGGT
    CATTTGGATG GTGTGTTTAT GCACAAAAGG GCTCAAAGAC TAAAATTCCT ATGTGAACGC
    AATGACAAGG TCTTCTTTGC CTCTGTTCGG TCTGGTGGCA GCAGCCAGGT TTATTTCATG
    ACGTTAGGCA GGACTTCTCT TCTGAGCTGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291554
    Clone ID Related Accession (Same CDS sequence) XM_022424571.1
    Accession Version XM_022424571.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3747bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGCAT
    GACCACAGGA GGCCGCACCC GCAGCAGCCG CCGCCACCGC AGCAGGAAAG GAGCAAGCCA
    AGCTATCACG CTCCGGAGCC TAAGCCCCAC TATGAGCCTG CTGACAGAGC TCGAGAGTGG
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CTCCCAAATT TGCACACCAC CATCTTCGCT CTCAGGACCC ATGTCCACCT
    TCCCGCAGTG AGGTGCTAAG TCAGAGTTCT GACTCTAAGT CGGAGGTACC TGACCCCACC
    CAAAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CAAGGGTTCC TGTGAGGACA
    ACGTCTCGTT CCCCTGTTCT GTCCCGTCGG GATTCCCCAC TGCAAGGCAG TGGACAGCAA
    AATAGTCAAG CAGGTCAAAG AAACTCCACT AGCAGTATTG AGCCCCGGCT GCTGTGGGAG
    AGAGTGGAGA AGCTGGTGCC CAGGCCTGGC AGTGGCAGCT CCTCCGGATC CAGCAACTCC
    GGATCCCAGC CTGGGTCCCA CCCTGGGTCC CAGAGTGGCT CTGGAGAGCG CTTCAGAGTG
    AGATCATCAT CCAAATCTGA AGGCTCTCCT TCTCAGCGCC TAGAAAATGC AGTGAAAAAA
    CCTGAAGAAA AGAAAGAAGT TTTCAGACCT CTCAAGCCTG CCGGCGAAGT GGATTTGACT
    GCACTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTGCGAC CACCACACAA AGTGACAGAC
    TACTCCTCAT CCAGTGAGGA GTCCGGGACA ACAGATGAGG AAGACGATGA TGTAGAACAA
    GAAGGGGCTG AGGAAGCCAC CTCTGGACCA GAGGACACCA GAGCAGCGTC GTCCCTGAAT
    TTGAGCAATG GTGAAACAGA ATCCGTGAAA ACCATGATTG TTCATGACGA CGTAGAAAGT
    GAACCAGCCA TGACCCCATC CAAGGAGGGC ACTCTAATCG TCCGCCAGAG TACAGTTGAC
    CAAAAGCGCG CCAGCCATCA TGAGAGCAAT GGCTTTGCCG GTCGCATTCA CCTCTTGCCA
    GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCCTCCTC CCCATCCTCC
    AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TACTAATGAG
    ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT
    ATAGACCCCA GATTACTACA GATTTCTCCA TCTAGTGGGA CAACAGTGAC TTCTGTGGTG
    GGATTTTCCT GTGATGGAAT GAGACCAGAA GCCATAAGGC AAGATCCTAC CCGGAAGGGC
    TCAGTGGTCA ATGTGAATCC CACCAACACT AGGCCACAGA GTGATACCCC GGAGATTCGT
    AAATATAAGA AGAGATTTAA CTCCGAGATT CTGTGTGCTG CCTTATGGGG AGTGAATTTG
    CTAGTGGGTA CAGAGAGTGG CCTGATGCTG CTGGACAGAA GTGGCCAAGG GAAGGTATAT
    CCCCTGATCA ACCGAAGACG ATTTCAGCAA ATGGATGTCC TTGAAGGCTT GAATGTCTTG
    GTGACAATAT CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTGTCCTG GTTAAGAAAT
    AAAATACTTC ACAATGATCC AGAAGTTGAG AAGAAGCAGG GATGGACGAC TGTGGGAGAT
    TTGGAAGGAT GTGTACACTA TAAAGTTGTA AAATATGAAA GAATCAAATT TCTGGTAATT
    GCTTTGAAGA GTTCTGTGGA GGTCTATGCG TGGGCACCCA AGCCATATCA CAAATTTATG
    GCCTTTAAGT CATTTGGGGA ATTAGTACAT AAGCCATTGC TGGTGGATCT CACTGTGGAG
    GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG
    GATTCTGGAT CAGTCTATGA CATTTATCTA CCAACACACG TAAGAAAGAA CCCACACTCT
    ATGATCCAGT GTAGCATCAA ACCCCATGCA ATCATCATCC TCCCCAACAC AGATGGCATG
    GAGCTTCTGG TGTGCTATGA AGATGAAGGG GTTTATGTGA ATACTTATGG AAGAATCACC
    AAGGATGTGG TTCTGCAGTG GGGAGAGATG CCAACATCTG TAGCATATAT TCGATCCAAC
    CAGACGATGG GCTGGGGAGA AAAGGCCATA GAGATCCGAT CTGTGGAAAC TGGTCATTTG
    GATGGTGTGT TTATGCACAA AAGGGCTCAA AGACTAAAAT TCCTATGTGA ACGCAATGAC
    AAGGTCTTCT TTGCCTCTGT TCGGTCTGGT GGCAGCAGCC AGGTTTATTT CATGACGTTA
    GGCAGGACTT CTCTTCTGAG CTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280279.1
    CDS175..3921
    Translation

    Target ORF information:

    RefSeq Version XM_022424571.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424571.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGCAT
    GACCACAGGA GGCCGCACCC GCAGCAGCCG CCGCCACCGC AGCAGGAAAG GAGCAAGCCA
    AGCTATCACG CTCCGGAGCC TAAGCCCCAC TATGAGCCTG CTGACAGAGC TCGAGAGTGG
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CTCCCAAATT TGCACACCAC CATCTTCGCT CTCAGGACCC ATGTCCACCT
    TCCCGCAGTG AGGTGCTAAG TCAGAGTTCT GACTCTAAGT CGGAGGTACC TGACCCCACC
    CAAAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CAAGGGTTCC TGTGAGGACA
    ACGTCTCGTT CCCCTGTTCT GTCCCGTCGG GATTCCCCAC TGCAAGGCAG TGGACAGCAA
    AATAGTCAAG CAGGTCAAAG AAACTCCACT AGCAGTATTG AGCCCCGGCT GCTGTGGGAG
    AGAGTGGAGA AGCTGGTGCC CAGGCCTGGC AGTGGCAGCT CCTCCGGATC CAGCAACTCC
    GGATCCCAGC CTGGGTCCCA CCCTGGGTCC CAGAGTGGCT CTGGAGAGCG CTTCAGAGTG
    AGATCATCAT CCAAATCTGA AGGCTCTCCT TCTCAGCGCC TAGAAAATGC AGTGAAAAAA
    CCTGAAGAAA AGAAAGAAGT TTTCAGACCT CTCAAGCCTG CCGGCGAAGT GGATTTGACT
    GCACTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTGCGAC CACCACACAA AGTGACAGAC
    TACTCCTCAT CCAGTGAGGA GTCCGGGACA ACAGATGAGG AAGACGATGA TGTAGAACAA
    GAAGGGGCTG AGGAAGCCAC CTCTGGACCA GAGGACACCA GAGCAGCGTC GTCCCTGAAT
    TTGAGCAATG GTGAAACAGA ATCCGTGAAA ACCATGATTG TTCATGACGA CGTAGAAAGT
    GAACCAGCCA TGACCCCATC CAAGGAGGGC ACTCTAATCG TCCGCCAGAG TACAGTTGAC
    CAAAAGCGCG CCAGCCATCA TGAGAGCAAT GGCTTTGCCG GTCGCATTCA CCTCTTGCCA
    GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCCTCCTC CCCATCCTCC
    AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TACTAATGAG
    ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT
    ATAGACCCCA GATTACTACA GATTTCTCCA TCTAGTGGGA CAACAGTGAC TTCTGTGGTG
    GGATTTTCCT GTGATGGAAT GAGACCAGAA GCCATAAGGC AAGATCCTAC CCGGAAGGGC
    TCAGTGGTCA ATGTGAATCC CACCAACACT AGGCCACAGA GTGATACCCC GGAGATTCGT
    AAATATAAGA AGAGATTTAA CTCCGAGATT CTGTGTGCTG CCTTATGGGG AGTGAATTTG
    CTAGTGGGTA CAGAGAGTGG CCTGATGCTG CTGGACAGAA GTGGCCAAGG GAAGGTATAT
    CCCCTGATCA ACCGAAGACG ATTTCAGCAA ATGGATGTCC TTGAAGGCTT GAATGTCTTG
    GTGACAATAT CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTGTCCTG GTTAAGAAAT
    AAAATACTTC ACAATGATCC AGAAGTTGAG AAGAAGCAGG GATGGACGAC TGTGGGAGAT
    TTGGAAGGAT GTGTACACTA TAAAGTTGTA AAATATGAAA GAATCAAATT TCTGGTAATT
    GCTTTGAAGA GTTCTGTGGA GGTCTATGCG TGGGCACCCA AGCCATATCA CAAATTTATG
    GCCTTTAAGT CATTTGGGGA ATTAGTACAT AAGCCATTGC TGGTGGATCT CACTGTGGAG
    GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG
    GATTCTGGAT CAGTCTATGA CATTTATCTA CCAACACACG TAAGAAAGAA CCCACACTCT
    ATGATCCAGT GTAGCATCAA ACCCCATGCA ATCATCATCC TCCCCAACAC AGATGGCATG
    GAGCTTCTGG TGTGCTATGA AGATGAAGGG GTTTATGTGA ATACTTATGG AAGAATCACC
    AAGGATGTGG TTCTGCAGTG GGGAGAGATG CCAACATCTG TAGCATATAT TCGATCCAAC
    CAGACGATGG GCTGGGGAGA AAAGGCCATA GAGATCCGAT CTGTGGAAAC TGGTCATTTG
    GATGGTGTGT TTATGCACAA AAGGGCTCAA AGACTAAAAT TCCTATGTGA ACGCAATGAC
    AAGGTCTTCT TTGCCTCTGT TCGGTCTGGT GGCAGCAGCC AGGTTTATTT CATGACGTTA
    GGCAGGACTT CTCTTCTGAG CTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291555
    Clone ID Related Accession (Same CDS sequence) XM_022424573.1
    Accession Version XM_022424573.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3699bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGCAT
    GACCACAGGA GGCCGCACCC GCAGCAGCCG CCGCCACCGC AGCAGGAAAG GAGCAAGCCA
    AGCTATCACG CTCCGGAGCC TAAGCCCCAC TATGAGCCTG CTGACAGAGC TCGAGAGGTG
    GAAGATAGAT TTAGGAAAAC TAACCACAGC TCCCCTGAAG CCCAGTCTAA GCAGACAGGC
    AGAGTATTGG AACCACCAGT GCCTTCCAGA TCAGAGTCTT TTTCCAATGG CAACTCCGAG
    TCTGTGCATC CTGCCCTGCA GAGACCAGCT GAGCCACAGG TACAGTGGTC CCACCTGGCA
    TCTCTCAAGA ACAATGTTTC CCCTGTCTCG CGATCCCATT CCTTCAGTGA CCCTTCTCCT
    CCCAAATTTG CACACCACCA TCTTCGCTCT CAGGACCCAT GTCCACCTTC CCGCAGTGAG
    GTGCTAAGTC AGAGTTCTGA CTCTAAGTCG GAGGTACCTG ACCCCACCCA AAAGGCTTGG
    TCTAGATCAG ACAGTGACGA GGTGCCTCCA AGGGTTCCTG TGAGGACAAC GTCTCGTTCC
    CCTGTTCTGT CCCGTCGGGA TTCCCCACTG CAAGGCAGTG GACAGCAAAA TAGTCAAGCA
    GGTCAAAGAA ACTCCACTAG CAGTATTGAG CCCCGGCTGC TGTGGGAGAG AGTGGAGAAG
    CTGGTGCCCA GGCCTGGCAG TGGCAGCTCC TCCGGATCCA GCAACTCCGG ATCCCAGCCT
    GGGTCCCACC CTGGGTCCCA GAGTGGCTCT GGAGAGCGCT TCAGAGTGAG ATCATCATCC
    AAATCTGAAG GCTCTCCTTC TCAGCGCCTA GAAAATGCAG TGAAAAAACC TGAAGAAAAG
    AAAGAAGTTT TCAGACCTCT CAAGCCTGCC GGCGAAGTGG ATTTGACTGC ACTGGCCAAA
    GAGCTTCGAG CAGTGGAAGA TGTGCGACCA CCACACAAAG TGACAGACTA CTCCTCATCC
    AGTGAGGAGT CCGGGACAAC AGATGAGGAA GACGATGATG TAGAACAAGA AGGGGCTGAG
    GAAGCCACCT CTGGACCAGA GGACACCAGA GCAGCGTCGT CCCTGAATTT GAGCAATGGT
    GAAACAGAAT CCGTGAAAAC CATGATTGTT CATGACGACG TAGAAAGTGA ACCAGCCATG
    ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT
    TCTCCATCTA GTGGGACAAC AGTGACTTCT GTGGTGGGAT TTTCCTGTGA TGGAATGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAGGGCTCAG TGGTCAATGT GAATCCCACC
    AACACTAGGC CACAGAGTGA TACCCCGGAG ATTCGTAAAT ATAAGAAGAG ATTTAACTCC
    GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG
    ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTATATCCCC TGATCAACCG AAGACGATTT
    CAGCAAATGG ATGTCCTTGA AGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA
    GTTGAGAAGA AGCAGGGATG GACGACTGTG GGAGATTTGG AAGGATGTGT ACACTATAAA
    GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTAATTGCTT TGAAGAGTTC TGTGGAGGTC
    TATGCGTGGG CACCCAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGGGAATTA
    GTACATAAGC CATTGCTGGT GGATCTCACT GTGGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CTGGATCAGT CTATGACATT
    TATCTACCAA CACACATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAC
    ACAGATGGCA TGGAGCTTCT GGTGTGCTAT GAAGATGAAG GGGTTTATGT GAATACTTAT
    GGAAGAATCA CCAAGGATGT GGTTCTGCAG TGGGGAGAGA TGCCAACATC TGTAGCATAT
    ATTCGATCCA ACCAGACGAT GGGCTGGGGA GAAAAGGCCA TAGAGATCCG ATCTGTGGAA
    ACTGGTCATT TGGATGGTGT GTTTATGCAC AAAAGGGCTC AAAGACTAAA ATTCCTATGT
    GAACGCAATG ACAAGGTCTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG CCAGGTTTAT
    TTCATGACGT TAGGCAGGAC TTCTCTTCTG AGCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280281.1
    CDS175..3873
    Translation

    Target ORF information:

    RefSeq Version XM_022424573.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424573.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGCAT
    GACCACAGGA GGCCGCACCC GCAGCAGCCG CCGCCACCGC AGCAGGAAAG GAGCAAGCCA
    AGCTATCACG CTCCGGAGCC TAAGCCCCAC TATGAGCCTG CTGACAGAGC TCGAGAGGTG
    GAAGATAGAT TTAGGAAAAC TAACCACAGC TCCCCTGAAG CCCAGTCTAA GCAGACAGGC
    AGAGTATTGG AACCACCAGT GCCTTCCAGA TCAGAGTCTT TTTCCAATGG CAACTCCGAG
    TCTGTGCATC CTGCCCTGCA GAGACCAGCT GAGCCACAGG TACAGTGGTC CCACCTGGCA
    TCTCTCAAGA ACAATGTTTC CCCTGTCTCG CGATCCCATT CCTTCAGTGA CCCTTCTCCT
    CCCAAATTTG CACACCACCA TCTTCGCTCT CAGGACCCAT GTCCACCTTC CCGCAGTGAG
    GTGCTAAGTC AGAGTTCTGA CTCTAAGTCG GAGGTACCTG ACCCCACCCA AAAGGCTTGG
    TCTAGATCAG ACAGTGACGA GGTGCCTCCA AGGGTTCCTG TGAGGACAAC GTCTCGTTCC
    CCTGTTCTGT CCCGTCGGGA TTCCCCACTG CAAGGCAGTG GACAGCAAAA TAGTCAAGCA
    GGTCAAAGAA ACTCCACTAG CAGTATTGAG CCCCGGCTGC TGTGGGAGAG AGTGGAGAAG
    CTGGTGCCCA GGCCTGGCAG TGGCAGCTCC TCCGGATCCA GCAACTCCGG ATCCCAGCCT
    GGGTCCCACC CTGGGTCCCA GAGTGGCTCT GGAGAGCGCT TCAGAGTGAG ATCATCATCC
    AAATCTGAAG GCTCTCCTTC TCAGCGCCTA GAAAATGCAG TGAAAAAACC TGAAGAAAAG
    AAAGAAGTTT TCAGACCTCT CAAGCCTGCC GGCGAAGTGG ATTTGACTGC ACTGGCCAAA
    GAGCTTCGAG CAGTGGAAGA TGTGCGACCA CCACACAAAG TGACAGACTA CTCCTCATCC
    AGTGAGGAGT CCGGGACAAC AGATGAGGAA GACGATGATG TAGAACAAGA AGGGGCTGAG
    GAAGCCACCT CTGGACCAGA GGACACCAGA GCAGCGTCGT CCCTGAATTT GAGCAATGGT
    GAAACAGAAT CCGTGAAAAC CATGATTGTT CATGACGACG TAGAAAGTGA ACCAGCCATG
    ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT
    TCTCCATCTA GTGGGACAAC AGTGACTTCT GTGGTGGGAT TTTCCTGTGA TGGAATGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAGGGCTCAG TGGTCAATGT GAATCCCACC
    AACACTAGGC CACAGAGTGA TACCCCGGAG ATTCGTAAAT ATAAGAAGAG ATTTAACTCC
    GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG
    ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTATATCCCC TGATCAACCG AAGACGATTT
    CAGCAAATGG ATGTCCTTGA AGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA
    GTTGAGAAGA AGCAGGGATG GACGACTGTG GGAGATTTGG AAGGATGTGT ACACTATAAA
    GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTAATTGCTT TGAAGAGTTC TGTGGAGGTC
    TATGCGTGGG CACCCAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGGGAATTA
    GTACATAAGC CATTGCTGGT GGATCTCACT GTGGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CTGGATCAGT CTATGACATT
    TATCTACCAA CACACATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAC
    ACAGATGGCA TGGAGCTTCT GGTGTGCTAT GAAGATGAAG GGGTTTATGT GAATACTTAT
    GGAAGAATCA CCAAGGATGT GGTTCTGCAG TGGGGAGAGA TGCCAACATC TGTAGCATAT
    ATTCGATCCA ACCAGACGAT GGGCTGGGGA GAAAAGGCCA TAGAGATCCG ATCTGTGGAA
    ACTGGTCATT TGGATGGTGT GTTTATGCAC AAAAGGGCTC AAAGACTAAA ATTCCTATGT
    GAACGCAATG ACAAGGTCTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG CCAGGTTTAT
    TTCATGACGT TAGGCAGGAC TTCTCTTCTG AGCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291556
    Clone ID Related Accession (Same CDS sequence) XM_022424574.1
    Accession Version XM_022424574.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3612bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTTCCTGTGA GGACAACGTC TCGTTCCCCT
    GTTCTGTCCC GTCGGGATTC CCCACTGCAA GGCAGTGGAC AGCAAAATAG TCAAGCAGGT
    CAAAGAAACT CCACTAGCAG TATTGAGCCC CGGCTGCTGT GGGAGAGAGT GGAGAAGCTG
    GTGCCCAGGC CTGGCAGTGG CAGCTCCTCC GGATCCAGCA ACTCCGGATC CCAGCCTGGG
    TCCCACCCTG GGTCCCAGAG TGGCTCTGGA GAGCGCTTCA GAGTGAGATC ATCATCCAAA
    TCTGAAGGCT CTCCTTCTCA GCGCCTAGAA AATGCAGTGA AAAAACCTGA AGAAAAGAAA
    GAAGTTTTCA GACCTCTCAA GCCTGCCGGC GAAGTGGATT TGACTGCACT GGCCAAAGAG
    CTTCGAGCAG TGGAAGATGT GCGACCACCA CACAAAGTGA CAGACTACTC CTCATCCAGT
    GAGGAGTCCG GGACAACAGA TGAGGAAGAC GATGATGTAG AACAAGAAGG GGCTGAGGAA
    GCCACCTCTG GACCAGAGGA CACCAGAGCA GCGTCGTCCC TGAATTTGAG CAATGGTGAA
    ACAGAATCCG TGAAAACCAT GATTGTTCAT GACGACGTAG AAAGTGAACC AGCCATGACC
    CCATCCAAGG AGGGCACTCT AATCGTCCGC CAGAGTACAG TTGACCAAAA GCGCGCCAGC
    CATCATGAGA GCAATGGCTT TGCCGGTCGC ATTCACCTCT TGCCAGATCT CTTACAGCAA
    AGCCATTCCT CCTCCACTTC CTCCACCTCC TCCTCCCCAT CCTCCAGCCA GCCGACACCC
    ACCATGTCCC CACAGACACC CCAGGACAAG CTCACTACTA ATGAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA
    CTACAGATTT CTCCATCTAG TGGGACAACA GTGACTTCTG TGGTGGGATT TTCCTGTGAT
    GGAATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AGGGCTCAGT GGTCAATGTG
    AATCCCACCA ACACTAGGCC ACAGAGTGAT ACCCCGGAGA TTCGTAAATA TAAGAAGAGA
    TTTAACTCCG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG
    AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TATATCCCCT GATCAACCGA
    AGACGATTTC AGCAAATGGA TGTCCTTGAA GGCTTGAATG TCTTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT
    GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACGACTGTGG GAGATTTGGA AGGATGTGTA
    CACTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TAATTGCTTT GAAGAGTTCT
    GTGGAGGTCT ATGCGTGGGC ACCCAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGGGAATTAG TACATAAGCC ATTGCTGGTG GATCTCACTG TGGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC TGGATCAGTC
    TATGACATTT ATCTACCAAC ACACGTAAGA AAGAACCCAC ACTCTATGAT CCAGTGTAGC
    ATCAAACCCC ATGCAATCAT CATCCTCCCC AACACAGATG GCATGGAGCT TCTGGTGTGC
    TATGAAGATG AAGGGGTTTA TGTGAATACT TATGGAAGAA TCACCAAGGA TGTGGTTCTG
    CAGTGGGGAG AGATGCCAAC ATCTGTAGCA TATATTCGAT CCAACCAGAC GATGGGCTGG
    GGAGAAAAGG CCATAGAGAT CCGATCTGTG GAAACTGGTC ATTTGGATGG TGTGTTTATG
    CACAAAAGGG CTCAAAGACT AAAATTCCTA TGTGAACGCA ATGACAAGGT CTTCTTTGCC
    TCTGTTCGGT CTGGTGGCAG CAGCCAGGTT TATTTCATGA CGTTAGGCAG GACTTCTCTT
    CTGAGCTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280282.1
    CDS175..3786
    Translation

    Target ORF information:

    RefSeq Version XM_022424574.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424574.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTTCCTGTGA GGACAACGTC TCGTTCCCCT
    GTTCTGTCCC GTCGGGATTC CCCACTGCAA GGCAGTGGAC AGCAAAATAG TCAAGCAGGT
    CAAAGAAACT CCACTAGCAG TATTGAGCCC CGGCTGCTGT GGGAGAGAGT GGAGAAGCTG
    GTGCCCAGGC CTGGCAGTGG CAGCTCCTCC GGATCCAGCA ACTCCGGATC CCAGCCTGGG
    TCCCACCCTG GGTCCCAGAG TGGCTCTGGA GAGCGCTTCA GAGTGAGATC ATCATCCAAA
    TCTGAAGGCT CTCCTTCTCA GCGCCTAGAA AATGCAGTGA AAAAACCTGA AGAAAAGAAA
    GAAGTTTTCA GACCTCTCAA GCCTGCCGGC GAAGTGGATT TGACTGCACT GGCCAAAGAG
    CTTCGAGCAG TGGAAGATGT GCGACCACCA CACAAAGTGA CAGACTACTC CTCATCCAGT
    GAGGAGTCCG GGACAACAGA TGAGGAAGAC GATGATGTAG AACAAGAAGG GGCTGAGGAA
    GCCACCTCTG GACCAGAGGA CACCAGAGCA GCGTCGTCCC TGAATTTGAG CAATGGTGAA
    ACAGAATCCG TGAAAACCAT GATTGTTCAT GACGACGTAG AAAGTGAACC AGCCATGACC
    CCATCCAAGG AGGGCACTCT AATCGTCCGC CAGAGTACAG TTGACCAAAA GCGCGCCAGC
    CATCATGAGA GCAATGGCTT TGCCGGTCGC ATTCACCTCT TGCCAGATCT CTTACAGCAA
    AGCCATTCCT CCTCCACTTC CTCCACCTCC TCCTCCCCAT CCTCCAGCCA GCCGACACCC
    ACCATGTCCC CACAGACACC CCAGGACAAG CTCACTACTA ATGAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA
    CTACAGATTT CTCCATCTAG TGGGACAACA GTGACTTCTG TGGTGGGATT TTCCTGTGAT
    GGAATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AGGGCTCAGT GGTCAATGTG
    AATCCCACCA ACACTAGGCC ACAGAGTGAT ACCCCGGAGA TTCGTAAATA TAAGAAGAGA
    TTTAACTCCG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG
    AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TATATCCCCT GATCAACCGA
    AGACGATTTC AGCAAATGGA TGTCCTTGAA GGCTTGAATG TCTTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT
    GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACGACTGTGG GAGATTTGGA AGGATGTGTA
    CACTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TAATTGCTTT GAAGAGTTCT
    GTGGAGGTCT ATGCGTGGGC ACCCAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGGGAATTAG TACATAAGCC ATTGCTGGTG GATCTCACTG TGGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC TGGATCAGTC
    TATGACATTT ATCTACCAAC ACACGTAAGA AAGAACCCAC ACTCTATGAT CCAGTGTAGC
    ATCAAACCCC ATGCAATCAT CATCCTCCCC AACACAGATG GCATGGAGCT TCTGGTGTGC
    TATGAAGATG AAGGGGTTTA TGTGAATACT TATGGAAGAA TCACCAAGGA TGTGGTTCTG
    CAGTGGGGAG AGATGCCAAC ATCTGTAGCA TATATTCGAT CCAACCAGAC GATGGGCTGG
    GGAGAAAAGG CCATAGAGAT CCGATCTGTG GAAACTGGTC ATTTGGATGG TGTGTTTATG
    CACAAAAGGG CTCAAAGACT AAAATTCCTA TGTGAACGCA ATGACAAGGT CTTCTTTGCC
    TCTGTTCGGT CTGGTGGCAG CAGCCAGGTT TATTTCATGA CGTTAGGCAG GACTTCTCTT
    CTGAGCTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291557
    Clone ID Related Accession (Same CDS sequence) XM_022424575.1
    Accession Version XM_022424575.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTTCCTGTGA GGACAACGTC TCGTTCCCCT
    GTTCTGTCCC GTCGGGATTC CCCACTGCAA GGCAGTGGAC AGCAAAATAG TCAAGCAGGT
    CAAAGAAACT CCACTAGTAT TGAGCCCCGG CTGCTGTGGG AGAGAGTGGA GAAGCTGGTG
    CCCAGGCCTG GCAGTGGCAG CTCCTCCGGA TCCAGCAACT CCGGATCCCA GCCTGGGTCC
    CACCCTGGGT CCCAGAGTGG CTCTGGAGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT
    GAAGGCTCTC CTTCTCAGCG CCTAGAAAAT GCAGTGAAAA AACCTGAAGA AAAGAAAGAA
    GTTTTCAGAC CTCTCAAGCC TGCCGGCGAA GTGGATTTGA CTGCACTGGC CAAAGAGCTT
    CGAGCAGTGG AAGATGTGCG ACCACCACAC AAAGTGACAG ACTACTCCTC ATCCAGTGAG
    GAGTCCGGGA CAACAGATGA GGAAGACGAT GATGTAGAAC AAGAAGGGGC TGAGGAAGCC
    ACCTCTGGAC CAGAGGACAC CAGAGCAGCG TCGTCCCTGA ATTTGAGCAA TGGTGAAACA
    GAATCCGTGA AAACCATGAT TGTTCATGAC GACGTAGAAA GTGAACCAGC CATGACCCCA
    TCCAAGGAGG GCACTCTAAT CGTCCGCCAG AGTACAGTTG ACCAAAAGCG CGCCAGCCAT
    CATGAGAGCA ATGGCTTTGC CGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC
    CATTCCTCCT CCACTTCCTC CACCTCCTCC TCCCCATCCT CCAGCCAGCC GACACCCACC
    ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTACTAATG AGACTCAGTC CGCTAGTAGC
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA
    CAGATTTCTC CATCTAGTGG GACAACAGTG ACTTCTGTGG TGGGATTTTC CTGTGATGGA
    ATGAGACCAG AAGCCATAAG GCAAGATCCT ACCCGGAAGG GCTCAGTGGT CAATGTGAAT
    CCCACCAACA CTAGGCCACA GAGTGATACC CCGGAGATTC GTAAATATAA GAAGAGATTT
    AACTCCGAGA TTCTGTGTGC TGCCTTATGG GGAGTGAATT TGCTAGTGGG TACAGAGAGT
    GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTAT ATCCCCTGAT CAACCGAAGA
    CGATTTCAGC AAATGGATGT CCTTGAAGGC TTGAATGTCT TGGTGACAAT ATCTGGCAAA
    AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAATGAT
    CCAGAAGTTG AGAAGAAGCA GGGATGGACG ACTGTGGGAG ATTTGGAAGG ATGTGTACAC
    TATAAAGTTG TAAAATATGA AAGAATCAAA TTTCTGGTAA TTGCTTTGAA GAGTTCTGTG
    GAGGTCTATG CGTGGGCACC CAAGCCATAT CACAAATTTA TGGCCTTTAA GTCATTTGGG
    GAATTAGTAC ATAAGCCATT GCTGGTGGAT CTCACTGTGG AGGAAGGCCA GAGGTTGAAA
    GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGATTCTGG ATCAGTCTAT
    GACATTTATC TACCAACACA CGTAAGAAAG AACCCACACT CTATGATCCA GTGTAGCATC
    AAACCCCATG CAATCATCAT CCTCCCCAAC ACAGATGGCA TGGAGCTTCT GGTGTGCTAT
    GAAGATGAAG GGGTTTATGT GAATACTTAT GGAAGAATCA CCAAGGATGT GGTTCTGCAG
    TGGGGAGAGA TGCCAACATC TGTAGCATAT ATTCGATCCA ACCAGACGAT GGGCTGGGGA
    GAAAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGTCATT TGGATGGTGT GTTTATGCAC
    AAAAGGGCTC AAAGACTAAA ATTCCTATGT GAACGCAATG ACAAGGTCTT CTTTGCCTCT
    GTTCGGTCTG GTGGCAGCAG CCAGGTTTAT TTCATGACGT TAGGCAGGAC TTCTCTTCTG
    AGCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280283.1
    CDS175..3783
    Translation

    Target ORF information:

    RefSeq Version XM_022424575.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424575.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTTCCTGTGA GGACAACGTC TCGTTCCCCT
    GTTCTGTCCC GTCGGGATTC CCCACTGCAA GGCAGTGGAC AGCAAAATAG TCAAGCAGGT
    CAAAGAAACT CCACTAGTAT TGAGCCCCGG CTGCTGTGGG AGAGAGTGGA GAAGCTGGTG
    CCCAGGCCTG GCAGTGGCAG CTCCTCCGGA TCCAGCAACT CCGGATCCCA GCCTGGGTCC
    CACCCTGGGT CCCAGAGTGG CTCTGGAGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT
    GAAGGCTCTC CTTCTCAGCG CCTAGAAAAT GCAGTGAAAA AACCTGAAGA AAAGAAAGAA
    GTTTTCAGAC CTCTCAAGCC TGCCGGCGAA GTGGATTTGA CTGCACTGGC CAAAGAGCTT
    CGAGCAGTGG AAGATGTGCG ACCACCACAC AAAGTGACAG ACTACTCCTC ATCCAGTGAG
    GAGTCCGGGA CAACAGATGA GGAAGACGAT GATGTAGAAC AAGAAGGGGC TGAGGAAGCC
    ACCTCTGGAC CAGAGGACAC CAGAGCAGCG TCGTCCCTGA ATTTGAGCAA TGGTGAAACA
    GAATCCGTGA AAACCATGAT TGTTCATGAC GACGTAGAAA GTGAACCAGC CATGACCCCA
    TCCAAGGAGG GCACTCTAAT CGTCCGCCAG AGTACAGTTG ACCAAAAGCG CGCCAGCCAT
    CATGAGAGCA ATGGCTTTGC CGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC
    CATTCCTCCT CCACTTCCTC CACCTCCTCC TCCCCATCCT CCAGCCAGCC GACACCCACC
    ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTACTAATG AGACTCAGTC CGCTAGTAGC
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA
    CAGATTTCTC CATCTAGTGG GACAACAGTG ACTTCTGTGG TGGGATTTTC CTGTGATGGA
    ATGAGACCAG AAGCCATAAG GCAAGATCCT ACCCGGAAGG GCTCAGTGGT CAATGTGAAT
    CCCACCAACA CTAGGCCACA GAGTGATACC CCGGAGATTC GTAAATATAA GAAGAGATTT
    AACTCCGAGA TTCTGTGTGC TGCCTTATGG GGAGTGAATT TGCTAGTGGG TACAGAGAGT
    GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTAT ATCCCCTGAT CAACCGAAGA
    CGATTTCAGC AAATGGATGT CCTTGAAGGC TTGAATGTCT TGGTGACAAT ATCTGGCAAA
    AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAATGAT
    CCAGAAGTTG AGAAGAAGCA GGGATGGACG ACTGTGGGAG ATTTGGAAGG ATGTGTACAC
    TATAAAGTTG TAAAATATGA AAGAATCAAA TTTCTGGTAA TTGCTTTGAA GAGTTCTGTG
    GAGGTCTATG CGTGGGCACC CAAGCCATAT CACAAATTTA TGGCCTTTAA GTCATTTGGG
    GAATTAGTAC ATAAGCCATT GCTGGTGGAT CTCACTGTGG AGGAAGGCCA GAGGTTGAAA
    GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGATTCTGG ATCAGTCTAT
    GACATTTATC TACCAACACA CGTAAGAAAG AACCCACACT CTATGATCCA GTGTAGCATC
    AAACCCCATG CAATCATCAT CCTCCCCAAC ACAGATGGCA TGGAGCTTCT GGTGTGCTAT
    GAAGATGAAG GGGTTTATGT GAATACTTAT GGAAGAATCA CCAAGGATGT GGTTCTGCAG
    TGGGGAGAGA TGCCAACATC TGTAGCATAT ATTCGATCCA ACCAGACGAT GGGCTGGGGA
    GAAAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGTCATT TGGATGGTGT GTTTATGCAC
    AAAAGGGCTC AAAGACTAAA ATTCCTATGT GAACGCAATG ACAAGGTCTT CTTTGCCTCT
    GTTCGGTCTG GTGGCAGCAG CCAGGTTTAT TTCATGACGT TAGGCAGGAC TTCTCTTCTG
    AGCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291558
    Clone ID Related Accession (Same CDS sequence) XM_022424576.1
    Accession Version XM_022424576.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGCAT
    GACCACAGGA GGCCGCACCC GCAGCAGCCG CCGCCACCGC AGCAGGAAAG GAGCAAGCCA
    AGCTATCACG CTCCGGAGCC TAAGCCCCAC TATGAGCCTG CTGACAGAGC TCGAGAGGTA
    CAGTGGTCCC ACCTGGCATC TCTCAAGAAC AATGTTTCCC CTGTCTCGCG ATCCCATTCC
    TTCAGTGACC CTTCTCCTCC CAAATTTGCA CACCACCATC TTCGCTCTCA GGACCCATGT
    CCACCTTCCC GCAGTGAGGT GCTAAGTCAG AGTTCTGACT CTAAGTCGGA GGTACCTGAC
    CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG
    AGGACAACGT CTCGTTCCCC TGTTCTGTCC CGTCGGGATT CCCCACTGCA AGGCAGTGGA
    CAGCAAAATA GTCAAGCAGG TCAAAGAAAC TCCACTAGCA GTATTGAGCC CCGGCTGCTG
    TGGGAGAGAG TGGAGAAGCT GGTGCCCAGG CCTGGCAGTG GCAGCTCCTC CGGATCCAGC
    AACTCCGGAT CCCAGCCTGG GTCCCACCCT GGGTCCCAGA GTGGCTCTGG AGAGCGCTTC
    AGAGTGAGAT CATCATCCAA ATCTGAAGGC TCTCCTTCTC AGCGCCTAGA AAATGCAGTG
    AAAAAACCTG AAGAAAAGAA AGAAGTTTTC AGACCTCTCA AGCCTGCCGG CGAAGTGGAT
    TTGACTGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGACCACC ACACAAAGTG
    ACAGACTACT CCTCATCCAG TGAGGAGTCC GGGACAACAG ATGAGGAAGA CGATGATGTA
    GAACAAGAAG GGGCTGAGGA AGCCACCTCT GGACCAGAGG ACACCAGAGC AGCGTCGTCC
    CTGAATTTGA GCAATGGTGA AACAGAATCC GTGAAAACCA TGATTGTTCA TGACGACGTA
    GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGATTAC TACAGATTTC TCCATCTAGT GGGACAACAG TGACTTCTGT GGTGGGATTT
    TCCTGTGATG GAATGAGACC AGAAGCCATA AGGCAAGATC CTACCCGGAA GGGCTCAGTG
    GTCAATGTGA ATCCCACCAA CACTAGGCCA CAGAGTGATA CCCCGGAGAT TCGTAAATAT
    AAGAAGAGAT TTAACTCCGA GATTCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG
    GGTACAGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT ATATCCCCTG
    ATCAACCGAA GACGATTTCA GCAAATGGAT GTCCTTGAAG GCTTGAATGT CTTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA
    CTTCACAATG ATCCAGAAGT TGAGAAGAAG CAGGGATGGA CGACTGTGGG AGATTTGGAA
    GGATGTGTAC ACTATAAAGT TGTAAAATAT GAAAGAATCA AATTTCTGGT AATTGCTTTG
    AAGAGTTCTG TGGAGGTCTA TGCGTGGGCA CCCAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GGGAATTAGT ACATAAGCCA TTGCTGGTGG ATCTCACTGT GGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCT
    GGATCAGTCT ATGACATTTA TCTACCAACA CACATCCAGT GTAGCATCAA ACCCCATGCA
    ATCATCATCC TCCCCAACAC AGATGGCATG GAGCTTCTGG TGTGCTATGA AGATGAAGGG
    GTTTATGTGA ATACTTATGG AAGAATCACC AAGGATGTGG TTCTGCAGTG GGGAGAGATG
    CCAACATCTG TAGCATATAT TCGATCCAAC CAGACGATGG GCTGGGGAGA AAAGGCCATA
    GAGATCCGAT CTGTGGAAAC TGGTCATTTG GATGGTGTGT TTATGCACAA AAGGGCTCAA
    AGACTAAAAT TCCTATGTGA ACGCAATGAC AAGGTCTTCT TTGCCTCTGT TCGGTCTGGT
    GGCAGCAGCC AGGTTTATTT CATGACGTTA GGCAGGACTT CTCTTCTGAG CTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280284.1
    CDS175..3711
    Translation

    Target ORF information:

    RefSeq Version XM_022424576.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424576.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGCAT
    GACCACAGGA GGCCGCACCC GCAGCAGCCG CCGCCACCGC AGCAGGAAAG GAGCAAGCCA
    AGCTATCACG CTCCGGAGCC TAAGCCCCAC TATGAGCCTG CTGACAGAGC TCGAGAGGTA
    CAGTGGTCCC ACCTGGCATC TCTCAAGAAC AATGTTTCCC CTGTCTCGCG ATCCCATTCC
    TTCAGTGACC CTTCTCCTCC CAAATTTGCA CACCACCATC TTCGCTCTCA GGACCCATGT
    CCACCTTCCC GCAGTGAGGT GCTAAGTCAG AGTTCTGACT CTAAGTCGGA GGTACCTGAC
    CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG
    AGGACAACGT CTCGTTCCCC TGTTCTGTCC CGTCGGGATT CCCCACTGCA AGGCAGTGGA
    CAGCAAAATA GTCAAGCAGG TCAAAGAAAC TCCACTAGCA GTATTGAGCC CCGGCTGCTG
    TGGGAGAGAG TGGAGAAGCT GGTGCCCAGG CCTGGCAGTG GCAGCTCCTC CGGATCCAGC
    AACTCCGGAT CCCAGCCTGG GTCCCACCCT GGGTCCCAGA GTGGCTCTGG AGAGCGCTTC
    AGAGTGAGAT CATCATCCAA ATCTGAAGGC TCTCCTTCTC AGCGCCTAGA AAATGCAGTG
    AAAAAACCTG AAGAAAAGAA AGAAGTTTTC AGACCTCTCA AGCCTGCCGG CGAAGTGGAT
    TTGACTGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGACCACC ACACAAAGTG
    ACAGACTACT CCTCATCCAG TGAGGAGTCC GGGACAACAG ATGAGGAAGA CGATGATGTA
    GAACAAGAAG GGGCTGAGGA AGCCACCTCT GGACCAGAGG ACACCAGAGC AGCGTCGTCC
    CTGAATTTGA GCAATGGTGA AACAGAATCC GTGAAAACCA TGATTGTTCA TGACGACGTA
    GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGATTAC TACAGATTTC TCCATCTAGT GGGACAACAG TGACTTCTGT GGTGGGATTT
    TCCTGTGATG GAATGAGACC AGAAGCCATA AGGCAAGATC CTACCCGGAA GGGCTCAGTG
    GTCAATGTGA ATCCCACCAA CACTAGGCCA CAGAGTGATA CCCCGGAGAT TCGTAAATAT
    AAGAAGAGAT TTAACTCCGA GATTCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG
    GGTACAGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT ATATCCCCTG
    ATCAACCGAA GACGATTTCA GCAAATGGAT GTCCTTGAAG GCTTGAATGT CTTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA
    CTTCACAATG ATCCAGAAGT TGAGAAGAAG CAGGGATGGA CGACTGTGGG AGATTTGGAA
    GGATGTGTAC ACTATAAAGT TGTAAAATAT GAAAGAATCA AATTTCTGGT AATTGCTTTG
    AAGAGTTCTG TGGAGGTCTA TGCGTGGGCA CCCAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GGGAATTAGT ACATAAGCCA TTGCTGGTGG ATCTCACTGT GGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCT
    GGATCAGTCT ATGACATTTA TCTACCAACA CACATCCAGT GTAGCATCAA ACCCCATGCA
    ATCATCATCC TCCCCAACAC AGATGGCATG GAGCTTCTGG TGTGCTATGA AGATGAAGGG
    GTTTATGTGA ATACTTATGG AAGAATCACC AAGGATGTGG TTCTGCAGTG GGGAGAGATG
    CCAACATCTG TAGCATATAT TCGATCCAAC CAGACGATGG GCTGGGGAGA AAAGGCCATA
    GAGATCCGAT CTGTGGAAAC TGGTCATTTG GATGGTGTGT TTATGCACAA AAGGGCTCAA
    AGACTAAAAT TCCTATGTGA ACGCAATGAC AAGGTCTTCT TTGCCTCTGT TCGGTCTGGT
    GGCAGCAGCC AGGTTTATTT CATGACGTTA GGCAGGACTT CTCTTCTGAG CTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291559
    Clone ID Related Accession (Same CDS sequence) XM_022424577.1
    Accession Version XM_022424577.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGCAT
    GACCACAGGA GGCCGCACCC GCAGCAGCCG CCGCCACCGC AGCAGGAAAG GAGCAAGCCA
    AGCTATCACG CTCCGGAGCC TAAGCCCCAC TATGAGCCTG CTGACAGAGC TCGAGAGGTT
    CCTGTGAGGA CAACGTCTCG TTCCCCTGTT CTGTCCCGTC GGGATTCCCC ACTGCAAGGC
    AGTGGACAGC AAAATAGTCA AGCAGGTCAA AGAAACTCCA CTAGCAGTAT TGAGCCCCGG
    CTGCTGTGGG AGAGAGTGGA GAAGCTGGTG CCCAGGCCTG GCAGTGGCAG CTCCTCCGGA
    TCCAGCAACT CCGGATCCCA GCCTGGGTCC CACCCTGGGT CCCAGAGTGG CTCTGGAGAG
    CGCTTCAGAG TGAGATCATC ATCCAAATCT GAAGGCTCTC CTTCTCAGCG CCTAGAAAAT
    GCAGTGAAAA AACCTGAAGA AAAGAAAGAA GTTTTCAGAC CTCTCAAGCC TGCCGGCGAA
    GTGGATTTGA CTGCACTGGC CAAAGAGCTT CGAGCAGTGG AAGATGTGCG ACCACCACAC
    AAAGTGACAG ACTACTCCTC ATCCAGTGAG GAGTCCGGGA CAACAGATGA GGAAGACGAT
    GATGTAGAAC AAGAAGGGGC TGAGGAAGCC ACCTCTGGAC CAGAGGACAC CAGAGCAGCG
    TCGTCCCTGA ATTTGAGCAA TGGTGAAACA GAATCCGTGA AAACCATGAT TGTTCATGAC
    GACGTAGAAA GTGAACCAGC CATGACCCCA TCCAAGGAGG GCACTCTAAT CGTCCGCCAG
    AGTACAGTTG ACCAAAAGCG CGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCATT
    CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC
    TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC
    ACTACTAATG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC
    TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGTGG GACAACAGTG
    ACTTCTGTGG TGGGATTTTC CTGTGATGGA ATGAGACCAG AAGCCATAAG GCAAGATCCT
    ACCCGGAAGG GCTCAGTGGT CAATGTGAAT CCCACCAACA CTAGGCCACA GAGTGATACC
    CCGGAGATTC GTAAATATAA GAAGAGATTT AACTCCGAGA TTCTGTGTGC TGCCTTATGG
    GGAGTGAATT TGCTAGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA
    GGGAAGGTAT ATCCCCTGAT CAACCGAAGA CGATTTCAGC AAATGGATGT CCTTGAAGGC
    TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC
    TGGTTAAGAA ATAAAATACT TCACAATGAT CCAGAAGTTG AGAAGAAGCA GGGATGGACG
    ACTGTGGGAG ATTTGGAAGG ATGTGTACAC TATAAAGTTG TAAAATATGA AAGAATCAAA
    TTTCTGGTAA TTGCTTTGAA GAGTTCTGTG GAGGTCTATG CGTGGGCACC CAAGCCATAT
    CACAAATTTA TGGCCTTTAA GTCATTTGGG GAATTAGTAC ATAAGCCATT GCTGGTGGAT
    CTCACTGTGG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT
    GCTGTTGATG TGGATTCTGG ATCAGTCTAT GACATTTATC TACCAACACA CGTAAGAAAG
    AACCCACACT CTATGATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAC
    ACAGATGGCA TGGAGCTTCT GGTGTGCTAT GAAGATGAAG GGGTTTATGT GAATACTTAT
    GGAAGAATCA CCAAGGATGT GGTTCTGCAG TGGGGAGAGA TGCCAACATC TGTAGCATAT
    ATTCGATCCA ACCAGACGAT GGGCTGGGGA GAAAAGGCCA TAGAGATCCG ATCTGTGGAA
    ACTGGTCATT TGGATGGTGT GTTTATGCAC AAAAGGGCTC AAAGACTAAA ATTCCTATGT
    GAACGCAATG ACAAGGTCTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG CCAGGTTTAT
    TTCATGACGT TAGGCAGGAC TTCTCTTCTG AGCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280285.1
    CDS175..3693
    Translation

    Target ORF information:

    RefSeq Version XM_022424577.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424577.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGCAT
    GACCACAGGA GGCCGCACCC GCAGCAGCCG CCGCCACCGC AGCAGGAAAG GAGCAAGCCA
    AGCTATCACG CTCCGGAGCC TAAGCCCCAC TATGAGCCTG CTGACAGAGC TCGAGAGGTT
    CCTGTGAGGA CAACGTCTCG TTCCCCTGTT CTGTCCCGTC GGGATTCCCC ACTGCAAGGC
    AGTGGACAGC AAAATAGTCA AGCAGGTCAA AGAAACTCCA CTAGCAGTAT TGAGCCCCGG
    CTGCTGTGGG AGAGAGTGGA GAAGCTGGTG CCCAGGCCTG GCAGTGGCAG CTCCTCCGGA
    TCCAGCAACT CCGGATCCCA GCCTGGGTCC CACCCTGGGT CCCAGAGTGG CTCTGGAGAG
    CGCTTCAGAG TGAGATCATC ATCCAAATCT GAAGGCTCTC CTTCTCAGCG CCTAGAAAAT
    GCAGTGAAAA AACCTGAAGA AAAGAAAGAA GTTTTCAGAC CTCTCAAGCC TGCCGGCGAA
    GTGGATTTGA CTGCACTGGC CAAAGAGCTT CGAGCAGTGG AAGATGTGCG ACCACCACAC
    AAAGTGACAG ACTACTCCTC ATCCAGTGAG GAGTCCGGGA CAACAGATGA GGAAGACGAT
    GATGTAGAAC AAGAAGGGGC TGAGGAAGCC ACCTCTGGAC CAGAGGACAC CAGAGCAGCG
    TCGTCCCTGA ATTTGAGCAA TGGTGAAACA GAATCCGTGA AAACCATGAT TGTTCATGAC
    GACGTAGAAA GTGAACCAGC CATGACCCCA TCCAAGGAGG GCACTCTAAT CGTCCGCCAG
    AGTACAGTTG ACCAAAAGCG CGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCATT
    CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC
    TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC
    ACTACTAATG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC
    TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGTGG GACAACAGTG
    ACTTCTGTGG TGGGATTTTC CTGTGATGGA ATGAGACCAG AAGCCATAAG GCAAGATCCT
    ACCCGGAAGG GCTCAGTGGT CAATGTGAAT CCCACCAACA CTAGGCCACA GAGTGATACC
    CCGGAGATTC GTAAATATAA GAAGAGATTT AACTCCGAGA TTCTGTGTGC TGCCTTATGG
    GGAGTGAATT TGCTAGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA
    GGGAAGGTAT ATCCCCTGAT CAACCGAAGA CGATTTCAGC AAATGGATGT CCTTGAAGGC
    TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC
    TGGTTAAGAA ATAAAATACT TCACAATGAT CCAGAAGTTG AGAAGAAGCA GGGATGGACG
    ACTGTGGGAG ATTTGGAAGG ATGTGTACAC TATAAAGTTG TAAAATATGA AAGAATCAAA
    TTTCTGGTAA TTGCTTTGAA GAGTTCTGTG GAGGTCTATG CGTGGGCACC CAAGCCATAT
    CACAAATTTA TGGCCTTTAA GTCATTTGGG GAATTAGTAC ATAAGCCATT GCTGGTGGAT
    CTCACTGTGG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT
    GCTGTTGATG TGGATTCTGG ATCAGTCTAT GACATTTATC TACCAACACA CGTAAGAAAG
    AACCCACACT CTATGATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAC
    ACAGATGGCA TGGAGCTTCT GGTGTGCTAT GAAGATGAAG GGGTTTATGT GAATACTTAT
    GGAAGAATCA CCAAGGATGT GGTTCTGCAG TGGGGAGAGA TGCCAACATC TGTAGCATAT
    ATTCGATCCA ACCAGACGAT GGGCTGGGGA GAAAAGGCCA TAGAGATCCG ATCTGTGGAA
    ACTGGTCATT TGGATGGTGT GTTTATGCAC AAAAGGGCTC AAAGACTAAA ATTCCTATGT
    GAACGCAATG ACAAGGTCTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG CCAGGTTTAT
    TTCATGACGT TAGGCAGGAC TTCTCTTCTG AGCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291560
    Clone ID Related Accession (Same CDS sequence) XM_022424578.1
    Accession Version XM_022424578.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGCAT
    GACCACAGGA GGCCGCACCC GCAGCAGCCG CCGCCACCGC AGCAGGAAAG GAGCAAGCCA
    AGCTATCACG CTCCGGAGCC TAAGCCCCAC TATGAGCCTG CTGACAGAGC TCGAGAGGTT
    CCTGTGAGGA CAACGTCTCG TTCCCCTGTT CTGTCCCGTC GGGATTCCCC ACTGCAAGGC
    AGTGGACAGC AAAATAGTCA AGCAGGTCAA AGAAACTCCA CTAGCAGTAT TGAGCCCCGG
    CTGCTGTGGG AGAGAGTGGA GAAGCTGGTG CCCAGGCCTG GCAGTGGCAG CTCCTCCGGA
    TCCAGCAACT CCGGATCCCA GCCTGGGTCC CACCCTGGGT CCCAGAGTGG CTCTGGAGAG
    CGCTTCAGAG TGAGATCATC ATCCAAATCT GAAGGCTCTC CTTCTCAGCG CCTAGAAAAT
    GCAGTGAAAA AACCTGAAGA AAAGAAAGAA GTTTTCAGAC CTCTCAAGCC TGCCGGCGAA
    GTGGATTTGA CTGCACTGGC CAAAGAGCTT CGAGCAGTGG AAGATGTGCG ACCACCACAC
    AAAGTGACAG ACTACTCCTC ATCCAGTGAG GAGTCCGGGA CAACAGATGA GGAAGACGAT
    GATGTAGAAC AAGAAGGGGC TGAGGAAGCC ACCTCTGGAC CAGAGGACAC CAGAGCAGCG
    TCGTCCCTGA ATTTGAGCAA TGGTGAAACA GAATCCGTGA AAACCATGAT TGTTCATGAC
    GACGTAGAAA GTGAACCAGC CATGACCCCA TCCAAGGAGG GCACTCTAAT CGTCCGCCAG
    AGTACAGTTG ACCAAAAGCG CGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCATT
    CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC
    TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC
    ACTACTAATG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC
    TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGTGG GACAACAGTG
    ACTTCTGTGG TGGGATTTTC CTGTGATGGA ATGAGACCAG AAGCCATAAG GCAAGATCCT
    ACCCGGAAGG GCTCAGTGGT CAATGTGAAT CCCACCAACA CTAGGCCACA GAGTGATACC
    CCGGAGATTC GTAAATATAA GAAGAGATTT AACTCCGAGA TTCTGTGTGC TGCCTTATGG
    GGAGTGAATT TGCTAGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA
    GGGAAGGTAT ATCCCCTGAT CAACCGAAGA CGATTTCAGC AAATGGATGT CCTTGAAGGC
    TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC
    TGGTTAAGAA ATAAAATACT TCACAATGAT CCAGAAGTTG AGAAGAAGCA GGGATGGACG
    ACTGTGGGAG ATTTGGAAGG ATGTGTACAC TATAAAGTTG TAAAATATGA AAGAATCAAA
    TTTCTGGTAA TTGCTTTGAA GAGTTCTGTG GAGGTCTATG CGTGGGCACC CAAGCCATAT
    CACAAATTTA TGGCCTTTAA GTCATTTGGG GAATTAGTAC ATAAGCCATT GCTGGTGGAT
    CTCACTGTGG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT
    GCTGTTGATG TGGATTCTGG ATCAGTCTAT GACATTTATC TACCAACACA CATCCAGTGT
    AGCATCAAAC CCCATGCAAT CATCATCCTC CCCAACACAG ATGGCATGGA GCTTCTGGTG
    TGCTATGAAG ATGAAGGGGT TTATGTGAAT ACTTATGGAA GAATCACCAA GGATGTGGTT
    CTGCAGTGGG GAGAGATGCC AACATCTGTA GCATATATTC GATCCAACCA GACGATGGGC
    TGGGGAGAAA AGGCCATAGA GATCCGATCT GTGGAAACTG GTCATTTGGA TGGTGTGTTT
    ATGCACAAAA GGGCTCAAAG ACTAAAATTC CTATGTGAAC GCAATGACAA GGTCTTCTTT
    GCCTCTGTTC GGTCTGGTGG CAGCAGCCAG GTTTATTTCA TGACGTTAGG CAGGACTTCT
    CTTCTGAGCT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280286.1
    CDS175..3669
    Translation

    Target ORF information:

    RefSeq Version XM_022424578.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424578.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGCAT
    GACCACAGGA GGCCGCACCC GCAGCAGCCG CCGCCACCGC AGCAGGAAAG GAGCAAGCCA
    AGCTATCACG CTCCGGAGCC TAAGCCCCAC TATGAGCCTG CTGACAGAGC TCGAGAGGTT
    CCTGTGAGGA CAACGTCTCG TTCCCCTGTT CTGTCCCGTC GGGATTCCCC ACTGCAAGGC
    AGTGGACAGC AAAATAGTCA AGCAGGTCAA AGAAACTCCA CTAGCAGTAT TGAGCCCCGG
    CTGCTGTGGG AGAGAGTGGA GAAGCTGGTG CCCAGGCCTG GCAGTGGCAG CTCCTCCGGA
    TCCAGCAACT CCGGATCCCA GCCTGGGTCC CACCCTGGGT CCCAGAGTGG CTCTGGAGAG
    CGCTTCAGAG TGAGATCATC ATCCAAATCT GAAGGCTCTC CTTCTCAGCG CCTAGAAAAT
    GCAGTGAAAA AACCTGAAGA AAAGAAAGAA GTTTTCAGAC CTCTCAAGCC TGCCGGCGAA
    GTGGATTTGA CTGCACTGGC CAAAGAGCTT CGAGCAGTGG AAGATGTGCG ACCACCACAC
    AAAGTGACAG ACTACTCCTC ATCCAGTGAG GAGTCCGGGA CAACAGATGA GGAAGACGAT
    GATGTAGAAC AAGAAGGGGC TGAGGAAGCC ACCTCTGGAC CAGAGGACAC CAGAGCAGCG
    TCGTCCCTGA ATTTGAGCAA TGGTGAAACA GAATCCGTGA AAACCATGAT TGTTCATGAC
    GACGTAGAAA GTGAACCAGC CATGACCCCA TCCAAGGAGG GCACTCTAAT CGTCCGCCAG
    AGTACAGTTG ACCAAAAGCG CGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCATT
    CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC
    TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC
    ACTACTAATG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC
    TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGTGG GACAACAGTG
    ACTTCTGTGG TGGGATTTTC CTGTGATGGA ATGAGACCAG AAGCCATAAG GCAAGATCCT
    ACCCGGAAGG GCTCAGTGGT CAATGTGAAT CCCACCAACA CTAGGCCACA GAGTGATACC
    CCGGAGATTC GTAAATATAA GAAGAGATTT AACTCCGAGA TTCTGTGTGC TGCCTTATGG
    GGAGTGAATT TGCTAGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA
    GGGAAGGTAT ATCCCCTGAT CAACCGAAGA CGATTTCAGC AAATGGATGT CCTTGAAGGC
    TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC
    TGGTTAAGAA ATAAAATACT TCACAATGAT CCAGAAGTTG AGAAGAAGCA GGGATGGACG
    ACTGTGGGAG ATTTGGAAGG ATGTGTACAC TATAAAGTTG TAAAATATGA AAGAATCAAA
    TTTCTGGTAA TTGCTTTGAA GAGTTCTGTG GAGGTCTATG CGTGGGCACC CAAGCCATAT
    CACAAATTTA TGGCCTTTAA GTCATTTGGG GAATTAGTAC ATAAGCCATT GCTGGTGGAT
    CTCACTGTGG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT
    GCTGTTGATG TGGATTCTGG ATCAGTCTAT GACATTTATC TACCAACACA CATCCAGTGT
    AGCATCAAAC CCCATGCAAT CATCATCCTC CCCAACACAG ATGGCATGGA GCTTCTGGTG
    TGCTATGAAG ATGAAGGGGT TTATGTGAAT ACTTATGGAA GAATCACCAA GGATGTGGTT
    CTGCAGTGGG GAGAGATGCC AACATCTGTA GCATATATTC GATCCAACCA GACGATGGGC
    TGGGGAGAAA AGGCCATAGA GATCCGATCT GTGGAAACTG GTCATTTGGA TGGTGTGTTT
    ATGCACAAAA GGGCTCAAAG ACTAAAATTC CTATGTGAAC GCAATGACAA GGTCTTCTTT
    GCCTCTGTTC GGTCTGGTGG CAGCAGCCAG GTTTATTTCA TGACGTTAGG CAGGACTTCT
    CTTCTGAGCT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38955
    Clone ID Related Accession (Same CDS sequence) XM_014117435.1 , XM_014117435.2
    Accession Version XM_014117435.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGGTACAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCTCCCAAAT TTGCACACCA CCATCTTCGC
    TCTCAGGACC CATGTCCACC TTCCCGCAGT GAGGTGCTAA GTCAGAGTTC TGACTCTAAG
    TCGGAGGTAC CTGACCCCAC CCAAAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT
    CCAAGGGTTC CTGTGAGGAC AACGTCTCGT TCCCCTGTTC TGTCCCGTCG GGATTCCCCA
    CTGCAAGGCA GTGGACAGCA AAATAGTCAA GCAGGTCAAA GAAACTCCAC TAGCAGTATT
    GAGCCCCGGC TGCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC
    TCCTCCGGAT CCAGCAACTC CGGATCCCAG CCTGGGTCCC ACCCTGGGTC CCAGAGTGGC
    TCTGGAGAGC GCTTCAGAGT GAGATCATCA TCCAAATCTG AAGGCTCTCC TTCTCAGCGC
    CTAGAAAATG CAGTGAAAAA ACCTGAAGAA AAGAAAGAAG TTTTCAGACC TCTCAAGCCT
    GCCGGCGAAG TGGATTTGAC TGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTGCGA
    CCACCACACA AAGTGACAGA CTACTCCTCA TCCAGTGAGG AGTCCGGGAC AACAGATGAG
    GAAGACGATG ATGTAGAACA AGAAGGGGCT GAGGAAGCCA CCTCTGGACC AGAGGACACC
    AGAGCAGCGT CGTCCCTGAA TTTGAGCAAT GGTGAAACAG AATCCGTGAA AACCATGATT
    GTTCATGACG ACGTAGAAAG TGAACCAGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC
    GTCCGCCAGA GTACAGTTGA CCAAAAGCGC GCCAGCCATC ATGAGAGCAA TGGCTTTGCC
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC
    ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG
    GACAAGCTCA CTACTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGTGGG
    ACAACAGTGA CTTCTGTGGT GGGATTTTCC TGTGATGGAA TGAGACCAGA AGCCATAAGG
    CAAGATCCTA CCCGGAAGGG CTCAGTGGTC AATGTGAATC CCACCAACAC TAGGCCACAG
    AGTGATACCC CGGAGATTCG TAAATATAAG AAGAGATTTA ACTCCGAGAT TCTGTGTGCT
    GCCTTATGGG GAGTGAATTT GCTAGTGGGT ACAGAGAGTG GCCTGATGCT GCTGGACAGA
    AGTGGCCAAG GGAAGGTATA TCCCCTGATC AACCGAAGAC GATTTCAGCA AATGGATGTC
    CTTGAAGGCT TGAATGTCTT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC
    TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAATGATC CAGAAGTTGA GAAGAAGCAG
    GGATGGACGA CTGTGGGAGA TTTGGAAGGA TGTGTACACT ATAAAGTTGT AAAATATGAA
    AGAATCAAAT TTCTGGTAAT TGCTTTGAAG AGTTCTGTGG AGGTCTATGC GTGGGCACCC
    AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGGG AATTAGTACA TAAGCCATTG
    CTGGTGGATC TCACTGTGGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT
    GGATTCCATG CTGTTGATGT GGATTCTGGA TCAGTCTATG ACATTTATCT ACCAACACAC
    ATCCAGTGTA GCATCAAACC CCATGCAATC ATCATCCTCC CCAACACAGA TGGCATGGAG
    CTTCTGGTGT GCTATGAAGA TGAAGGGGTT TATGTGAATA CTTATGGAAG AATCACCAAG
    GATGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATATATTCG ATCCAACCAG
    ACGATGGGCT GGGGAGAAAA GGCCATAGAG ATCCGATCTG TGGAAACTGG TCATTTGGAT
    GGTGTGTTTA TGCACAAAAG GGCTCAAAGA CTAAAATTCC TATGTGAACG CAATGACAAG
    GTCTTCTTTG CCTCTGTTCG GTCTGGTGGC AGCAGCCAGG TTTATTTCAT GACGTTAGGC
    AGGACTTCTC TTCTGAGCTG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972910.1
    CDS118..4101
    Translation

    Target ORF information:

    RefSeq Version XM_014117435.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117435.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGGTACAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCTCCCAAAT TTGCACACCA CCATCTTCGC
    TCTCAGGACC CATGTCCACC TTCCCGCAGT GAGGTGCTAA GTCAGAGTTC TGACTCTAAG
    TCGGAGGTAC CTGACCCCAC CCAAAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT
    CCAAGGGTTC CTGTGAGGAC AACGTCTCGT TCCCCTGTTC TGTCCCGTCG GGATTCCCCA
    CTGCAAGGCA GTGGACAGCA AAATAGTCAA GCAGGTCAAA GAAACTCCAC TAGCAGTATT
    GAGCCCCGGC TGCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC
    TCCTCCGGAT CCAGCAACTC CGGATCCCAG CCTGGGTCCC ACCCTGGGTC CCAGAGTGGC
    TCTGGAGAGC GCTTCAGAGT GAGATCATCA TCCAAATCTG AAGGCTCTCC TTCTCAGCGC
    CTAGAAAATG CAGTGAAAAA ACCTGAAGAA AAGAAAGAAG TTTTCAGACC TCTCAAGCCT
    GCCGGCGAAG TGGATTTGAC TGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTGCGA
    CCACCACACA AAGTGACAGA CTACTCCTCA TCCAGTGAGG AGTCCGGGAC AACAGATGAG
    GAAGACGATG ATGTAGAACA AGAAGGGGCT GAGGAAGCCA CCTCTGGACC AGAGGACACC
    AGAGCAGCGT CGTCCCTGAA TTTGAGCAAT GGTGAAACAG AATCCGTGAA AACCATGATT
    GTTCATGACG ACGTAGAAAG TGAACCAGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC
    GTCCGCCAGA GTACAGTTGA CCAAAAGCGC GCCAGCCATC ATGAGAGCAA TGGCTTTGCC
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC
    ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG
    GACAAGCTCA CTACTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGTGGG
    ACAACAGTGA CTTCTGTGGT GGGATTTTCC TGTGATGGAA TGAGACCAGA AGCCATAAGG
    CAAGATCCTA CCCGGAAGGG CTCAGTGGTC AATGTGAATC CCACCAACAC TAGGCCACAG
    AGTGATACCC CGGAGATTCG TAAATATAAG AAGAGATTTA ACTCCGAGAT TCTGTGTGCT
    GCCTTATGGG GAGTGAATTT GCTAGTGGGT ACAGAGAGTG GCCTGATGCT GCTGGACAGA
    AGTGGCCAAG GGAAGGTATA TCCCCTGATC AACCGAAGAC GATTTCAGCA AATGGATGTC
    CTTGAAGGCT TGAATGTCTT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC
    TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAATGATC CAGAAGTTGA GAAGAAGCAG
    GGATGGACGA CTGTGGGAGA TTTGGAAGGA TGTGTACACT ATAAAGTTGT AAAATATGAA
    AGAATCAAAT TTCTGGTAAT TGCTTTGAAG AGTTCTGTGG AGGTCTATGC GTGGGCACCC
    AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGGG AATTAGTACA TAAGCCATTG
    CTGGTGGATC TCACTGTGGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT
    GGATTCCATG CTGTTGATGT GGATTCTGGA TCAGTCTATG ACATTTATCT ACCAACACAC
    ATCCAGTGTA GCATCAAACC CCATGCAATC ATCATCCTCC CCAACACAGA TGGCATGGAG
    CTTCTGGTGT GCTATGAAGA TGAAGGGGTT TATGTGAATA CTTATGGAAG AATCACCAAG
    GATGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATATATTCG ATCCAACCAG
    ACGATGGGCT GGGGAGAAAA GGCCATAGAG ATCCGATCTG TGGAAACTGG TCATTTGGAT
    GGTGTGTTTA TGCACAAAAG GGCTCAAAGA CTAAAATTCC TATGTGAACG CAATGACAAG
    GTCTTCTTTG CCTCTGTTCG GTCTGGTGGC AGCAGCCAGG TTTATTTCAT GACGTTAGGC
    AGGACTTCTC TTCTGAGCTG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38955
    Clone ID Related Accession (Same CDS sequence) XM_014117435.1 , XM_014117435.2
    Accession Version XM_014117435.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117435.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGGTACAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCTCCCAAAT TTGCACACCA CCATCTTCGC
    TCTCAGGACC CATGTCCACC TTCCCGCAGT GAGGTGCTAA GTCAGAGTTC TGACTCTAAG
    TCGGAGGTAC CTGACCCCAC CCAAAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT
    CCAAGGGTTC CTGTGAGGAC AACGTCTCGT TCCCCTGTTC TGTCCCGTCG GGATTCCCCA
    CTGCAAGGCA GTGGACAGCA AAATAGTCAA GCAGGTCAAA GAAACTCCAC TAGCAGTATT
    GAGCCCCGGC TGCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC
    TCCTCCGGAT CCAGCAACTC CGGATCCCAG CCTGGGTCCC ACCCTGGGTC CCAGAGTGGC
    TCTGGAGAGC GCTTCAGAGT GAGATCATCA TCCAAATCTG AAGGCTCTCC TTCTCAGCGC
    CTAGAAAATG CAGTGAAAAA ACCTGAAGAA AAGAAAGAAG TTTTCAGACC TCTCAAGCCT
    GCCGGCGAAG TGGATTTGAC TGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTGCGA
    CCACCACACA AAGTGACAGA CTACTCCTCA TCCAGTGAGG AGTCCGGGAC AACAGATGAG
    GAAGACGATG ATGTAGAACA AGAAGGGGCT GAGGAAGCCA CCTCTGGACC AGAGGACACC
    AGAGCAGCGT CGTCCCTGAA TTTGAGCAAT GGTGAAACAG AATCCGTGAA AACCATGATT
    GTTCATGACG ACGTAGAAAG TGAACCAGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC
    GTCCGCCAGA GTACAGTTGA CCAAAAGCGC GCCAGCCATC ATGAGAGCAA TGGCTTTGCC
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC
    ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG
    GACAAGCTCA CTACTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGTGGG
    ACAACAGTGA CTTCTGTGGT GGGATTTTCC TGTGATGGAA TGAGACCAGA AGCCATAAGG
    CAAGATCCTA CCCGGAAGGG CTCAGTGGTC AATGTGAATC CCACCAACAC TAGGCCACAG
    AGTGATACCC CGGAGATTCG TAAATATAAG AAGAGATTTA ACTCCGAGAT TCTGTGTGCT
    GCCTTATGGG GAGTGAATTT GCTAGTGGGT ACAGAGAGTG GCCTGATGCT GCTGGACAGA
    AGTGGCCAAG GGAAGGTATA TCCCCTGATC AACCGAAGAC GATTTCAGCA AATGGATGTC
    CTTGAAGGCT TGAATGTCTT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC
    TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAATGATC CAGAAGTTGA GAAGAAGCAG
    GGATGGACGA CTGTGGGAGA TTTGGAAGGA TGTGTACACT ATAAAGTTGT AAAATATGAA
    AGAATCAAAT TTCTGGTAAT TGCTTTGAAG AGTTCTGTGG AGGTCTATGC GTGGGCACCC
    AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGGG AATTAGTACA TAAGCCATTG
    CTGGTGGATC TCACTGTGGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT
    GGATTCCATG CTGTTGATGT GGATTCTGGA TCAGTCTATG ACATTTATCT ACCAACACAC
    ATCCAGTGTA GCATCAAACC CCATGCAATC ATCATCCTCC CCAACACAGA TGGCATGGAG
    CTTCTGGTGT GCTATGAAGA TGAAGGGGTT TATGTGAATA CTTATGGAAG AATCACCAAG
    GATGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATATATTCG ATCCAACCAG
    ACGATGGGCT GGGGAGAAAA GGCCATAGAG ATCCGATCTG TGGAAACTGG TCATTTGGAT
    GGTGTGTTTA TGCACAAAAG GGCTCAAAGA CTAAAATTCC TATGTGAACG CAATGACAAG
    GTCTTCTTTG CCTCTGTTCG GTCTGGTGGC AGCAGCCAGG TTTATTTCAT GACGTTAGGC
    AGGACTTCTC TTCTGAGCTG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972910.1
    CDS175..4158
    Translation

    Target ORF information:

    RefSeq Version XM_014117435.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117435.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTCA CTGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAT TATGGTGCTT TCATTAAAAA GAGCCCTCCA
    GGACATGATG ACCAACTCTG GCTTGTTATG GAGTTCTGTG GGGCTGGGTC CATTACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAGGAAGACT GGATAGCTTA CATCTCCAGA
    GAAATCCTGA GGGGACTGGC ACATCTTCAC ATCCATCACG TGATTCACCG AGACATCAAG
    GGCCAGAATG TGTTGCTGAC CGAGAATGCA GAGGTGAAGC TTGTTGATTT CGGCGTGAGT
    GCTCAGCTTG ACCGGACCGT TGGGAGGAGA AATACGTTCA TAGGCACCCC CTACTGGATG
    GCTCCTGAGG TTATTGCCTG TGATGAGAAC CCAGATGCCA CCTATGATTA CAGAAGTGAT
    CTTTGGTCTT GTGGCATCAC AGCCATTGAG ATGGCAGAAG GTGCTCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCACT GTTTCTCATT CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTTAGTTTT ATAGAAGGGT GCCTGGTGAA GAATTACATG
    CAGCGACCCT CCACAGAGCA GCTTTTGAAA CATCCTTTTA TAAGGGATCA GCCAAACGAA
    AGGCAAGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGAAAGAA GAGAGGAGAG
    AAAGATGAAA CCGAATATGA GTACAGTGGG AGTGAGGAAG AAGAGGAGGA AGTGCCTGAA
    CAGGAAGGAG AGCCAAGCTC CATTGTCAAT GTGCCTGGTG AGTCGACACT TCGTCGGGAT
    TTCTTGAGAC TGCAGCAGGA GAACAAGGAA CGTTCTGAGG CTCTTCGGAG GCAGCAGCTA
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAA GAGTATAAGA GGCAGCTACT GGCAGAGAGG
    CAGAAACGCA TCGAGCAGCA GAAAGAACAG AGGCGGCGAC TAGAAGAGCA ACAAAGGAGA
    GAGCGGGAAG CTAGAAGGCA ACAAGAACGT GAACAGCGAA GGAGAGAACA AGAAGAGAAG
    AGGCGTCTGG AGGAACTGGA GAGAAGACGT AAAGAGGAAG AAGAGAGGAG GCGGGCAGAG
    GAAGAAAAGA GGAGAGTTGA AAGAGAACAG GAGTATATCA GGCGACAGCT AGAAGAGGAG
    CAGCGGCACT TGGAAATCCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GTTACTGGAG
    TGCCGATGGC GGGAGATGGA GGAGCACCGG CAGGCAGAGA GGCTCCAGAG GCAGTTGCAA
    CAAGAACAAG CATATCTCCT GTCTCTACAG CATGACCACA GGAGGCCGCA CCCGCAGCAG
    CCGCCGCCAC CGCAGCAGGA AAGGAGCAAG CCAAGCTATC ACGCTCCGGA GCCTAAGCCC
    CACTATGAGC CTGCTGACAG AGCTCGAGAG GTGGAAGATA GATTTAGGAA AACTAACCAC
    AGCTCCCCTG AAGCCCAGTC TAAGCAGACA GGCAGAGTAT TGGAACCACC AGTGCCTTCC
    AGATCAGAGT CTTTTTCCAA TGGCAACTCC GAGTCTGTGC ATCCTGCCCT GCAGAGACCA
    GCTGAGCCAC AGGTACAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCTCCCAAAT TTGCACACCA CCATCTTCGC
    TCTCAGGACC CATGTCCACC TTCCCGCAGT GAGGTGCTAA GTCAGAGTTC TGACTCTAAG
    TCGGAGGTAC CTGACCCCAC CCAAAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT
    CCAAGGGTTC CTGTGAGGAC AACGTCTCGT TCCCCTGTTC TGTCCCGTCG GGATTCCCCA
    CTGCAAGGCA GTGGACAGCA AAATAGTCAA GCAGGTCAAA GAAACTCCAC TAGCAGTATT
    GAGCCCCGGC TGCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGGCCTGG CAGTGGCAGC
    TCCTCCGGAT CCAGCAACTC CGGATCCCAG CCTGGGTCCC ACCCTGGGTC CCAGAGTGGC
    TCTGGAGAGC GCTTCAGAGT GAGATCATCA TCCAAATCTG AAGGCTCTCC TTCTCAGCGC
    CTAGAAAATG CAGTGAAAAA ACCTGAAGAA AAGAAAGAAG TTTTCAGACC TCTCAAGCCT
    GCCGGCGAAG TGGATTTGAC TGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTGCGA
    CCACCACACA AAGTGACAGA CTACTCCTCA TCCAGTGAGG AGTCCGGGAC AACAGATGAG
    GAAGACGATG ATGTAGAACA AGAAGGGGCT GAGGAAGCCA CCTCTGGACC AGAGGACACC
    AGAGCAGCGT CGTCCCTGAA TTTGAGCAAT GGTGAAACAG AATCCGTGAA AACCATGATT
    GTTCATGACG ACGTAGAAAG TGAACCAGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC
    GTCCGCCAGA GTACAGTTGA CCAAAAGCGC GCCAGCCATC ATGAGAGCAA TGGCTTTGCC
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC
    ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG
    GACAAGCTCA CTACTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGTGGG
    ACAACAGTGA CTTCTGTGGT GGGATTTTCC TGTGATGGAA TGAGACCAGA AGCCATAAGG
    CAAGATCCTA CCCGGAAGGG CTCAGTGGTC AATGTGAATC CCACCAACAC TAGGCCACAG
    AGTGATACCC CGGAGATTCG TAAATATAAG AAGAGATTTA ACTCCGAGAT TCTGTGTGCT
    GCCTTATGGG GAGTGAATTT GCTAGTGGGT ACAGAGAGTG GCCTGATGCT GCTGGACAGA
    AGTGGCCAAG GGAAGGTATA TCCCCTGATC AACCGAAGAC GATTTCAGCA AATGGATGTC
    CTTGAAGGCT TGAATGTCTT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC
    TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAATGATC CAGAAGTTGA GAAGAAGCAG
    GGATGGACGA CTGTGGGAGA TTTGGAAGGA TGTGTACACT ATAAAGTTGT AAAATATGAA
    AGAATCAAAT TTCTGGTAAT TGCTTTGAAG AGTTCTGTGG AGGTCTATGC GTGGGCACCC
    AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGGG AATTAGTACA TAAGCCATTG
    CTGGTGGATC TCACTGTGGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT
    GGATTCCATG CTGTTGATGT GGATTCTGGA TCAGTCTATG ACATTTATCT ACCAACACAC
    ATCCAGTGTA GCATCAAACC CCATGCAATC ATCATCCTCC CCAACACAGA TGGCATGGAG
    CTTCTGGTGT GCTATGAAGA TGAAGGGGTT TATGTGAATA CTTATGGAAG AATCACCAAG
    GATGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATATATTCG ATCCAACCAG
    ACGATGGGCT GGGGAGAAAA GGCCATAGAG ATCCGATCTG TGGAAACTGG TCATTTGGAT
    GGTGTGTTTA TGCACAAAAG GGCTCAAAGA CTAAAATTCC TATGTGAACG CAATGACAAG
    GTCTTCTTTG CCTCTGTTCG GTCTGGTGGC AGCAGCCAGG TTTATTTCAT GACGTTAGGC
    AGGACTTCTC TTCTGAGCTG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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