Map4k4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Map4k4
Entrez Gene ID	301363
Full Name	mitogen-activated protein kinase kinase kinase kinase 4
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase kinase 4 Names mitogen-activated protein kinase kinase kinase kinase 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q22

mRNA and Protein(s)

mRNA	Protein	Name
XM_008767005.2	XP_008765227.2	mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
XM_017596340.1	XP_017451829.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
XM_008767010.2	XP_008765232.2	mitogen-activated protein kinase kinase kinase kinase 4 isoform X12
XM_017596342.1	XP_017451831.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X16
NM_001106904.1	NP_001100374.1	mitogen-activated protein kinase kinase kinase kinase 4
XM_008767003.2	XP_008765225.2	mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
XM_008767004.2	XP_008765226.2	mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
XM_008767007.2	XP_008765229.2	mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
XM_008767012.2	XP_008765234.2	mitogen-activated protein kinase kinase kinase kinase 4 isoform X15
XM_017596345.1	XP_017451834.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X21
XM_008767015.2	XP_008765237.2	mitogen-activated protein kinase kinase kinase kinase 4 isoform X22
XM_008767016.2	XP_008765238.2	mitogen-activated protein kinase kinase kinase kinase 4 isoform X23
XM_008767001.2	XP_008765223.2	mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
XM_008767002.2	XP_008765224.2	mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
XM_008767006.2	XP_008765228.2	mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
XM_008767008.2	XP_008765230.2	mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
XM_008767009.2	XP_008765231.2	mitogen-activated protein kinase kinase kinase kinase 4 isoform X11
XM_017596344.1	XP_017451833.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X19
XM_008767014.2	XP_008765236.2	mitogen-activated protein kinase kinase kinase kinase 4 isoform X20
XM_008767011.2	XP_008765233.2	mitogen-activated protein kinase kinase kinase kinase 4 isoform X14
XM_017596343.1	XP_017451832.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X17
XM_008767013.2	XP_008765235.2	mitogen-activated protein kinase kinase kinase kinase 4 isoform X18
XM_017596346.1	XP_017451835.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X24
XM_008767000.2	XP_008765222.2	mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
XM_017596341.1	XP_017451830.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X13

Pathways from BioSystems

Homologies

	MAP4K4	XP_001107420.2
Bos taurus (cattle)	MAP4K4	XP_003586662.2
Canis lupus familiaris (dog)	MAP4K4	XP_005626291.1
Homo sapiens (human)	MAP4K4	NP_663719.2
Rattus norvegicus (Norway rat)	Map4k4	NP_001100374.1
Xenopus tropicalis (tropical clawed frog)	map4k4	XP_002934552.1
Pan troglodytes (chimpanzee)	MAP4K4	XP_515665.3
Mus musculus (house mouse)	Map4k4	NP_001239129.1
Gallus gallus (chicken)	MAP4K4	XP_416913.4
Danio rerio (zebrafish)	wu:fa03e11	XP_001343426.5

Gene Ontology
Bibliography

Related articles in PubMed

The Nck-interacting kinase NIK increases Arp2/3 complex activity by phosphorylating the Arp2 subunit.
LeClaire LL, Rana M, Baumgartner M, Barber DL
The Journal of cell biology208(2)161-70(2015 Jan)

Microtubules regulate focal adhesion dynamics through MAP4K4.
Yue J, Xie M, Gou X, Lee P, Schneider MD, Wu X
Developmental cell31(5)572-85(2014 Dec)

Identification of Map4k4 as a novel suppressor of skeletal muscle differentiation.
Wang M, Amano SU, Flach RJ, Chawla A, Aouadi M, Czech MP
Molecular and cellular biology33(4)678-87(2013 Feb)

A Novel Interaction between Pyk2 and MAP4K4 Is Integrated with Glioma Cell Migration.
Loftus JC, Yang Z, Kloss J, Dhruv H, Tran NL, Riggs DL
Journal of signal transduction2013956580(2013)

Rap2A links intestinal cell polarity to brush border formation.
Gloerich M, ten Klooster JP, Vliem MJ, Koorman T, Zwartkruis FJ, Clevers H, Bos JL
Nature cell biology14(8)793-801(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NIK is the first kinase shown to phosphorylate and increase the activity of the Arp2/3 complex.
Title: The Nck-interacting kinase NIK increases Arp2/3 complex activity by phosphorylating the Arp2 subunit.

Results identify MAP4K4 as a key upstream mediator of TNF-alpha action on the beta cell, preventing tumor necrosis factor-alpha-induced decrease of IRS-2 and inhibition of glucose-stimulated insulin secretion.
Title: Silencing mitogen-activated protein 4 kinase 4 (MAP4K4) protects beta cells from tumor necrosis factor-alpha-induced decrease of IRS-2 and inhibition of glucose-stimulated insulin secretion.

The following Map4k4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Map4k4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa62690	XM_008767000.2 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa62691	XM_008767001.2 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa62692	XM_008767002.2 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa62693	XM_008767003.2 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa62694	XM_008767004.2 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa62695	XM_008767005.2 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa62696	XM_008767006.2 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa62697	XM_017596340.1 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa62698	XM_008767007.2 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62699	XM_008767008.2 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62700	XM_008767009.2 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62701	XM_008767010.2 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62702	XM_017596341.1 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62703	XM_008767011.2 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62704	XM_008767012.2 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62705	XM_017596342.1 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62706	XM_017596343.1 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62707	XM_008767013.2 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62708	XM_017596344.1 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62709	XM_008767014.2 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62710	XM_017596345.1 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62711	XM_008767015.2 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62712	XM_008767016.2 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62713	XM_017596346.1 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa42317	NM_001106904.1 Latest version!	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Map4k4 ( XM_008767000.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765222.2 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa62690
Clone ID Related Accession (Same CDS sequence)	XM_008767000.2
Accession Version	XM_008767000.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767000.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG 
GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT 
TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG 
GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT 
TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT 
CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG 
CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG 
AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG 
CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA 
TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC 
AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGG AGAAGTGGAC 
TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG 
ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG 
GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT 
CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA 
GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGAGTACA 
GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC 
TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC TTCCTCCCCA 
TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT 
AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
CCTTTTATAG ACCCCAGGTT ACTACAGATC TCTCCATCTA GTGGAACAAC AGTTACTTCC 
GTTGTGGGAT TTTCCTGTGA TGGGCTGAGA CCAGAAGCCA TAAGGCAAGA TCCTACTCGG 
AAAGGCTCAG TGGTCAATGT GAACCCCACG AACACTAGGC CACAGAGTGA TACCCCCGAG 
ATTCGTAAAT ACAAGAAGAG ATTTAACTCA GAGATCCTGT GTGCTGCCTT ATGGGGAGTG 
AATTTGCTAG TTGGCACAGA GAGTGGCCTG ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA 
GTGTACCCTC TGATCAGCCG GAGACGGTTC CAGCAAATGG ATGTACTTGA GGGCCTGAAT 
GTCCTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGCG TCTACTATTT ATCCTGGTTA 
AGAAATAAAA TACTTCATAA TGATCCGGAA GTTGAGAAGA AGCAGGGGTG GACCACCGTG 
GGCGACCTGG AAGGGTGCGT GCACTATAAA GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG 
GTAATCGCTT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CACCGAAGCC ATATCACAAA 
TTTATGGCCT TTAAGTCATT TGGAGAACTG GTACATAAGC CATTACTGGT GGATCTCACT 
GTCGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
GATGTGGATT CCGGCTCAGT CTATGACATT TATCTACCAA CACACGTAAG AAAGAACCCA 
CACTCTATGA TTCAGTGTAC CATCAAACCC CATGCAATCA TTATCCTCCC CAACACGGAT 
GGAATGGAGC TTCTGGTGTG CTACGAAGAT GAAGGGGTTT ATGTGAACAC GTACGGAAGA 
ATCACCAAGG ACGTGGTTCT GCAGTGGGGA GAGATGCCAA CATCTGTAGC ATACATCCGG 
TCGAATCAGA CAATGGGCTG GGGAGAGAAG GCAATAGAGA TACGCTCTGT GGAAACTGGT 
CATTTGGACG GTGTGTTTAT GCACAAAAGG GCTCAGAGAC TAAAGTTCCT GTGTGAACGT 
AATGACAAGG TGTTCTTTGC TTCCGTCCGC TCTGGCGGCA GCAGCCAGGT CTACTTCATG 
ACCTTAGGCA GGACTTCCCT TCTGAGCTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765222.2
CDS	211..4383
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVEDRFRKTNHSSPEAQAKQTGRGLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008767000.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767000.2

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG 
GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT 
TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG 
GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT 
TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT 
CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG 
CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG 
AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG 
CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA 
TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC 
AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGG AGAAGTGGAC 
TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG 
ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG 
GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT 
CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA 
GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGAGTACA 
GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC 
TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC TTCCTCCCCA 
TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT 
AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
CCTTTTATAG ACCCCAGGTT ACTACAGATC TCTCCATCTA GTGGAACAAC AGTTACTTCC 
GTTGTGGGAT TTTCCTGTGA TGGGCTGAGA CCAGAAGCCA TAAGGCAAGA TCCTACTCGG 
AAAGGCTCAG TGGTCAATGT GAACCCCACG AACACTAGGC CACAGAGTGA TACCCCCGAG 
ATTCGTAAAT ACAAGAAGAG ATTTAACTCA GAGATCCTGT GTGCTGCCTT ATGGGGAGTG 
AATTTGCTAG TTGGCACAGA GAGTGGCCTG ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA 
GTGTACCCTC TGATCAGCCG GAGACGGTTC CAGCAAATGG ATGTACTTGA GGGCCTGAAT 
GTCCTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGCG TCTACTATTT ATCCTGGTTA 
AGAAATAAAA TACTTCATAA TGATCCGGAA GTTGAGAAGA AGCAGGGGTG GACCACCGTG 
GGCGACCTGG AAGGGTGCGT GCACTATAAA GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG 
GTAATCGCTT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CACCGAAGCC ATATCACAAA 
TTTATGGCCT TTAAGTCATT TGGAGAACTG GTACATAAGC CATTACTGGT GGATCTCACT 
GTCGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
GATGTGGATT CCGGCTCAGT CTATGACATT TATCTACCAA CACACGTAAG AAAGAACCCA 
CACTCTATGA TTCAGTGTAC CATCAAACCC CATGCAATCA TTATCCTCCC CAACACGGAT 
GGAATGGAGC TTCTGGTGTG CTACGAAGAT GAAGGGGTTT ATGTGAACAC GTACGGAAGA 
ATCACCAAGG ACGTGGTTCT GCAGTGGGGA GAGATGCCAA CATCTGTAGC ATACATCCGG 
TCGAATCAGA CAATGGGCTG GGGAGAGAAG GCAATAGAGA TACGCTCTGT GGAAACTGGT 
CATTTGGACG GTGTGTTTAT GCACAAAAGG GCTCAGAGAC TAAAGTTCCT GTGTGAACGT 
AATGACAAGG TGTTCTTTGC TTCCGTCCGC TCTGGCGGCA GCAGCCAGGT CTACTTCATG 
ACCTTAGGCA GGACTTCCCT TCTGAGCTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_008767001.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765223.2 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa62691
Clone ID Related Accession (Same CDS sequence)	XM_008767001.2
Accession Version	XM_008767001.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG 
GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT 
TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG 
GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT 
TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT 
CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG 
CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG 
AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG 
CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGTA TTGAGCCCCG GCTTCTATGG 
GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC 
TCAGGATCCC AGCCTGGCTC CCATCCTGGG TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA 
GTGAGATCAT CATCCAAGTC TGAAGGTTCT CCATCTCAGC GCCTTGAAAA TGCAGCCAAA 
AAACCTGAAG ATAAGAAAGA AGTATTTCGA CCTCTCAAAC CTGCTGGAGA AGTGGACTTG 
ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTGC GGCCACCTCA CAAAGTGACA 
GACTATTCCT CCTCCAGTGA GGAGTCTGGG ACCACGGATG AGGAGGAGGA GGATGTGGAA 
CAGGAGGGGG CTGATGATTC CACCTCGGGG CCAGAGGACA CCAGAGCAGC GTCGTCTCTG 
AATCTGAGCA ACGGTGAGAC AGAATCTGTG AAAACAATGA TTGTTCATGA TGACGTAGAA 
AGTGAACCAG CCATGACCCC ATCCAAGGAG GGCACCCTAA TCGTCCGCCA GAGTACAGTT 
GACCAAAAGC GTGCCAGCCA TCATGAGAGC AATGGCTTTG CGGGTCGCAT TCACCTCTTG 
CCAGATCTCT TACAGCAAAG CCATTCCTCC TCCACTTCCT CCACCTCTTC CTCCCCATCC 
TCCAGCCAGC CGACACCCAC CATGTCCCCA CAGACACCCC AGGACAAGCT CACTGCTAAT 
GAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT 
TTTATAGACC CCAGGTTACT ACAGATCTCT CCATCTAGTG GAACAACAGT TACTTCCGTT 
GTGGGATTTT CCTGTGATGG GCTGAGACCA GAAGCCATAA GGCAAGATCC TACTCGGAAA 
GGCTCAGTGG TCAATGTGAA CCCCACGAAC ACTAGGCCAC AGAGTGATAC CCCCGAGATT 
CGTAAATACA AGAAGAGATT TAACTCAGAG ATCCTGTGTG CTGCCTTATG GGGAGTGAAT 
TTGCTAGTTG GCACAGAGAG TGGCCTGATG CTCTTGGACA GAAGTGGCCA AGGGAAAGTG 
TACCCTCTGA TCAGCCGGAG ACGGTTCCAG CAAATGGATG TACTTGAGGG CCTGAATGTC 
CTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGCGTCT ACTATTTATC CTGGTTAAGA 
AATAAAATAC TTCATAATGA TCCGGAAGTT GAGAAGAAGC AGGGGTGGAC CACCGTGGGC 
GACCTGGAAG GGTGCGTGCA CTATAAAGTC GTAAAATATG AAAGAATCAA GTTTCTGGTA 
ATCGCTTTGA AGAGTTCTGT GGAAGTCTAT GCATGGGCAC CGAAGCCATA TCACAAATTT 
ATGGCCTTTA AGTCATTTGG AGAACTGGTA CATAAGCCAT TACTGGTGGA TCTCACTGTC 
GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT 
GTGGATTCCG GCTCAGTCTA TGACATTTAT CTACCAACAC ACGTAAGAAA GAACCCACAC 
TCTATGATTC AGTGTACCAT CAAACCCCAT GCAATCATTA TCCTCCCCAA CACGGATGGA 
ATGGAGCTTC TGGTGTGCTA CGAAGATGAA GGGGTTTATG TGAACACGTA CGGAAGAATC 
ACCAAGGACG TGGTTCTGCA GTGGGGAGAG ATGCCAACAT CTGTAGCATA CATCCGGTCG 
AATCAGACAA TGGGCTGGGG AGAGAAGGCA ATAGAGATAC GCTCTGTGGA AACTGGTCAT 
TTGGACGGTG TGTTTATGCA CAAAAGGGCT CAGAGACTAA AGTTCCTGTG TGAACGTAAT 
GACAAGGTGT TCTTTGCTTC CGTCCGCTCT GGCGGCAGCA GCCAGGTCTA CTTCATGACC 
TTAGGCAGGA CTTCCCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765223.2
CDS	211..4380
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVEDRFRKTNHSSPEAQAKQTGRGLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008767001.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767001.2

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG 
GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT 
TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG 
GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT 
TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT 
CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG 
CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG 
AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG 
CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGTA TTGAGCCCCG GCTTCTATGG 
GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC 
TCAGGATCCC AGCCTGGCTC CCATCCTGGG TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA 
GTGAGATCAT CATCCAAGTC TGAAGGTTCT CCATCTCAGC GCCTTGAAAA TGCAGCCAAA 
AAACCTGAAG ATAAGAAAGA AGTATTTCGA CCTCTCAAAC CTGCTGGAGA AGTGGACTTG 
ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTGC GGCCACCTCA CAAAGTGACA 
GACTATTCCT CCTCCAGTGA GGAGTCTGGG ACCACGGATG AGGAGGAGGA GGATGTGGAA 
CAGGAGGGGG CTGATGATTC CACCTCGGGG CCAGAGGACA CCAGAGCAGC GTCGTCTCTG 
AATCTGAGCA ACGGTGAGAC AGAATCTGTG AAAACAATGA TTGTTCATGA TGACGTAGAA 
AGTGAACCAG CCATGACCCC ATCCAAGGAG GGCACCCTAA TCGTCCGCCA GAGTACAGTT 
GACCAAAAGC GTGCCAGCCA TCATGAGAGC AATGGCTTTG CGGGTCGCAT TCACCTCTTG 
CCAGATCTCT TACAGCAAAG CCATTCCTCC TCCACTTCCT CCACCTCTTC CTCCCCATCC 
TCCAGCCAGC CGACACCCAC CATGTCCCCA CAGACACCCC AGGACAAGCT CACTGCTAAT 
GAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT 
TTTATAGACC CCAGGTTACT ACAGATCTCT CCATCTAGTG GAACAACAGT TACTTCCGTT 
GTGGGATTTT CCTGTGATGG GCTGAGACCA GAAGCCATAA GGCAAGATCC TACTCGGAAA 
GGCTCAGTGG TCAATGTGAA CCCCACGAAC ACTAGGCCAC AGAGTGATAC CCCCGAGATT 
CGTAAATACA AGAAGAGATT TAACTCAGAG ATCCTGTGTG CTGCCTTATG GGGAGTGAAT 
TTGCTAGTTG GCACAGAGAG TGGCCTGATG CTCTTGGACA GAAGTGGCCA AGGGAAAGTG 
TACCCTCTGA TCAGCCGGAG ACGGTTCCAG CAAATGGATG TACTTGAGGG CCTGAATGTC 
CTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGCGTCT ACTATTTATC CTGGTTAAGA 
AATAAAATAC TTCATAATGA TCCGGAAGTT GAGAAGAAGC AGGGGTGGAC CACCGTGGGC 
GACCTGGAAG GGTGCGTGCA CTATAAAGTC GTAAAATATG AAAGAATCAA GTTTCTGGTA 
ATCGCTTTGA AGAGTTCTGT GGAAGTCTAT GCATGGGCAC CGAAGCCATA TCACAAATTT 
ATGGCCTTTA AGTCATTTGG AGAACTGGTA CATAAGCCAT TACTGGTGGA TCTCACTGTC 
GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT 
GTGGATTCCG GCTCAGTCTA TGACATTTAT CTACCAACAC ACGTAAGAAA GAACCCACAC 
TCTATGATTC AGTGTACCAT CAAACCCCAT GCAATCATTA TCCTCCCCAA CACGGATGGA 
ATGGAGCTTC TGGTGTGCTA CGAAGATGAA GGGGTTTATG TGAACACGTA CGGAAGAATC 
ACCAAGGACG TGGTTCTGCA GTGGGGAGAG ATGCCAACAT CTGTAGCATA CATCCGGTCG 
AATCAGACAA TGGGCTGGGG AGAGAAGGCA ATAGAGATAC GCTCTGTGGA AACTGGTCAT 
TTGGACGGTG TGTTTATGCA CAAAAGGGCT CAGAGACTAA AGTTCCTGTG TGAACGTAAT 
GACAAGGTGT TCTTTGCTTC CGTCCGCTCT GGCGGCAGCA GCCAGGTCTA CTTCATGACC 
TTAGGCAGGA CTTCCCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_008767002.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765224.2 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa62692
Clone ID Related Accession (Same CDS sequence)	XM_008767002.2
Accession Version	XM_008767002.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767002.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG 
GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT 
TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG 
TGGTCCCACC TGGCATCTCT CAAGAACAAT GTCTCCCCTG TCTCGAGATC CCATTCCTTC 
AGTGACCCTT CTCCCAAATT CGCACACCAC CATCTCCGCT CTCAGGACCC ATGTCCACCT 
TCCCGCAGTG AGGGGCTCAG TCAGAGCTCT GACTCTAAGT CGGAGGTACC TGAGCCCACC 
CAAAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CAAGGGTTCC TGTGAGAACG 
ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA 
AATAGCCAAG CAGGACAGAG AAATTCCACC AGCAGTATTG AGCCCCGGCT TCTATGGGAG 
AGAGTGGAAA AGCTGGTCCC CAGGCCAGGC AGTGGCAGCT CTTCAGGGTC CAGCAACTCA 
GGATCCCAGC CTGGCTCCCA TCCTGGGTCT CAGAGTGGCT CTGGTGAACG CTTCAGAGTG 
AGATCATCAT CCAAGTCTGA AGGTTCTCCA TCTCAGCGCC TTGAAAATGC AGCCAAAAAA 
CCTGAAGATA AGAAAGAAGT ATTTCGACCT CTCAAACCTG CTGGAGAAGT GGACTTGACT 
GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTGCGGC CACCTCACAA AGTGACAGAC 
TATTCCTCCT CCAGTGAGGA GTCTGGGACC ACGGATGAGG AGGAGGAGGA TGTGGAACAG 
GAGGGGGCTG ATGATTCCAC CTCGGGGCCA GAGGACACCA GAGCAGCGTC GTCTCTGAAT 
CTGAGCAACG GTGAGACAGA ATCTGTGAAA ACAATGATTG TTCATGATGA CGTAGAAAGT 
GAACCAGCCA TGACCCCATC CAAGGAGGGC ACCCTAATCG TCCGCCAGAG TACAGTTGAC 
CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA 
GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCTTCCTC CCCATCCTCC 
AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG 
ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
ATAGACCCCA GGTTACTACA GATCTCTCCA TCTAGTGGAA CAACAGTTAC TTCCGTTGTG 
GGATTTTCCT GTGATGGGCT GAGACCAGAA GCCATAAGGC AAGATCCTAC TCGGAAAGGC 
TCAGTGGTCA ATGTGAACCC CACGAACACT AGGCCACAGA GTGATACCCC CGAGATTCGT 
AAATACAAGA AGAGATTTAA CTCAGAGATC CTGTGTGCTG CCTTATGGGG AGTGAATTTG 
CTAGTTGGCA CAGAGAGTGG CCTGATGCTC TTGGACAGAA GTGGCCAAGG GAAAGTGTAC 
CCTCTGATCA GCCGGAGACG GTTCCAGCAA ATGGATGTAC TTGAGGGCCT GAATGTCCTG 
GTGACAATAT CTGGCAAAAA GGATAAGTTA CGCGTCTACT ATTTATCCTG GTTAAGAAAT 
AAAATACTTC ATAATGATCC GGAAGTTGAG AAGAAGCAGG GGTGGACCAC CGTGGGCGAC 
CTGGAAGGGT GCGTGCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTAATC 
GCTTTGAAGA GTTCTGTGGA AGTCTATGCA TGGGCACCGA AGCCATATCA CAAATTTATG 
GCCTTTAAGT CATTTGGAGA ACTGGTACAT AAGCCATTAC TGGTGGATCT CACTGTCGAG 
GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG 
GATTCCGGCT CAGTCTATGA CATTTATCTA CCAACACACG TAAGAAAGAA CCCACACTCT 
ATGATTCAGT GTACCATCAA ACCCCATGCA ATCATTATCC TCCCCAACAC GGATGGAATG 
GAGCTTCTGG TGTGCTACGA AGATGAAGGG GTTTATGTGA ACACGTACGG AAGAATCACC 
AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCAACATCTG TAGCATACAT CCGGTCGAAT 
CAGACAATGG GCTGGGGAGA GAAGGCAATA GAGATACGCT CTGTGGAAAC TGGTCATTTG 
GACGGTGTGT TTATGCACAA AAGGGCTCAG AGACTAAAGT TCCTGTGTGA ACGTAATGAC 
AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC GGCAGCAGCC AGGTCTACTT CATGACCTTA 
GGCAGGACTT CCCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765224.2
CDS	211..4377
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVEDRFRKTNHSSPEAQAKQTGRGLEPPVPSRSESFSNGNSESVHPALQRPAEPQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008767002.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767002.2

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG 
GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT 
TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG 
TGGTCCCACC TGGCATCTCT CAAGAACAAT GTCTCCCCTG TCTCGAGATC CCATTCCTTC 
AGTGACCCTT CTCCCAAATT CGCACACCAC CATCTCCGCT CTCAGGACCC ATGTCCACCT 
TCCCGCAGTG AGGGGCTCAG TCAGAGCTCT GACTCTAAGT CGGAGGTACC TGAGCCCACC 
CAAAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CAAGGGTTCC TGTGAGAACG 
ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA 
AATAGCCAAG CAGGACAGAG AAATTCCACC AGCAGTATTG AGCCCCGGCT TCTATGGGAG 
AGAGTGGAAA AGCTGGTCCC CAGGCCAGGC AGTGGCAGCT CTTCAGGGTC CAGCAACTCA 
GGATCCCAGC CTGGCTCCCA TCCTGGGTCT CAGAGTGGCT CTGGTGAACG CTTCAGAGTG 
AGATCATCAT CCAAGTCTGA AGGTTCTCCA TCTCAGCGCC TTGAAAATGC AGCCAAAAAA 
CCTGAAGATA AGAAAGAAGT ATTTCGACCT CTCAAACCTG CTGGAGAAGT GGACTTGACT 
GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTGCGGC CACCTCACAA AGTGACAGAC 
TATTCCTCCT CCAGTGAGGA GTCTGGGACC ACGGATGAGG AGGAGGAGGA TGTGGAACAG 
GAGGGGGCTG ATGATTCCAC CTCGGGGCCA GAGGACACCA GAGCAGCGTC GTCTCTGAAT 
CTGAGCAACG GTGAGACAGA ATCTGTGAAA ACAATGATTG TTCATGATGA CGTAGAAAGT 
GAACCAGCCA TGACCCCATC CAAGGAGGGC ACCCTAATCG TCCGCCAGAG TACAGTTGAC 
CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA 
GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCTTCCTC CCCATCCTCC 
AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG 
ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
ATAGACCCCA GGTTACTACA GATCTCTCCA TCTAGTGGAA CAACAGTTAC TTCCGTTGTG 
GGATTTTCCT GTGATGGGCT GAGACCAGAA GCCATAAGGC AAGATCCTAC TCGGAAAGGC 
TCAGTGGTCA ATGTGAACCC CACGAACACT AGGCCACAGA GTGATACCCC CGAGATTCGT 
AAATACAAGA AGAGATTTAA CTCAGAGATC CTGTGTGCTG CCTTATGGGG AGTGAATTTG 
CTAGTTGGCA CAGAGAGTGG CCTGATGCTC TTGGACAGAA GTGGCCAAGG GAAAGTGTAC 
CCTCTGATCA GCCGGAGACG GTTCCAGCAA ATGGATGTAC TTGAGGGCCT GAATGTCCTG 
GTGACAATAT CTGGCAAAAA GGATAAGTTA CGCGTCTACT ATTTATCCTG GTTAAGAAAT 
AAAATACTTC ATAATGATCC GGAAGTTGAG AAGAAGCAGG GGTGGACCAC CGTGGGCGAC 
CTGGAAGGGT GCGTGCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTAATC 
GCTTTGAAGA GTTCTGTGGA AGTCTATGCA TGGGCACCGA AGCCATATCA CAAATTTATG 
GCCTTTAAGT CATTTGGAGA ACTGGTACAT AAGCCATTAC TGGTGGATCT CACTGTCGAG 
GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG 
GATTCCGGCT CAGTCTATGA CATTTATCTA CCAACACACG TAAGAAAGAA CCCACACTCT 
ATGATTCAGT GTACCATCAA ACCCCATGCA ATCATTATCC TCCCCAACAC GGATGGAATG 
GAGCTTCTGG TGTGCTACGA AGATGAAGGG GTTTATGTGA ACACGTACGG AAGAATCACC 
AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCAACATCTG TAGCATACAT CCGGTCGAAT 
CAGACAATGG GCTGGGGAGA GAAGGCAATA GAGATACGCT CTGTGGAAAC TGGTCATTTG 
GACGGTGTGT TTATGCACAA AAGGGCTCAG AGACTAAAGT TCCTGTGTGA ACGTAATGAC 
AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC GGCAGCAGCC AGGTCTACTT CATGACCTTA 
GGCAGGACTT CCCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_008767003.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765225.2 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa62693
Clone ID Related Accession (Same CDS sequence)	XM_008767003.2
Accession Version	XM_008767003.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG 
GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT 
TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG 
GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT 
TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT 
CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG 
CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG 
AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG 
CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA 
TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC 
AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGA CTTGACTGCG 
TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTGCGGCCAC CTCACAAAGT GACAGACTAT 
TCCTCCTCCA GTGAGGAGTC TGGGACCACG GATGAGGAGG AGGAGGATGT GGAACAGGAG 
GGGGCTGATG ATTCCACCTC GGGGCCAGAG GACACCAGAG CAGCGTCGTC TCTGAATCTG 
AGCAACGGTG AGACAGAATC TGTGAAAACA ATGATTGTTC ATGATGACGT AGAAAGTGAA 
CCAGCCATGA CCCCATCCAA GGAGGGCACC CTAATCGTCC GCCAGAGTAC AGTTGACCAA 
AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCGGGTC GCATTCACCT CTTGCCAGAT 
CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CTTCCTCCCC ATCCTCCAGC 
CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT 
CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
GACCCCAGGT TACTACAGAT CTCTCCATCT AGTGGAACAA CAGTTACTTC CGTTGTGGGA 
TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC ATAAGGCAAG ATCCTACTCG GAAAGGCTCA 
GTGGTCAATG TGAACCCCAC GAACACTAGG CCACAGAGTG ATACCCCCGA GATTCGTAAA 
TACAAGAAGA GATTTAACTC AGAGATCCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA 
GTTGGCACAG AGAGTGGCCT GATGCTCTTG GACAGAAGTG GCCAAGGGAA AGTGTACCCT 
CTGATCAGCC GGAGACGGTT CCAGCAAATG GATGTACTTG AGGGCCTGAA TGTCCTGGTG 
ACAATATCTG GCAAAAAGGA TAAGTTACGC GTCTACTATT TATCCTGGTT AAGAAATAAA 
ATACTTCATA ATGATCCGGA AGTTGAGAAG AAGCAGGGGT GGACCACCGT GGGCGACCTG 
GAAGGGTGCG TGCACTATAA AGTCGTAAAA TATGAAAGAA TCAAGTTTCT GGTAATCGCT 
TTGAAGAGTT CTGTGGAAGT CTATGCATGG GCACCGAAGC CATATCACAA ATTTATGGCC 
TTTAAGTCAT TTGGAGAACT GGTACATAAG CCATTACTGG TGGATCTCAC TGTCGAGGAA 
GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT 
TCCGGCTCAG TCTATGACAT TTATCTACCA ACACACGTAA GAAAGAACCC ACACTCTATG 
ATTCAGTGTA CCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGAATGGAG 
CTTCTGGTGT GCTACGAAGA TGAAGGGGTT TATGTGAACA CGTACGGAAG AATCACCAAG 
GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATACATCCG GTCGAATCAG 
ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGCTCTG TGGAAACTGG TCATTTGGAC 
GGTGTGTTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG TAATGACAAG 
GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC AGCAGCCAGG TCTACTTCAT GACCTTAGGC 
AGGACTTCCC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765225.2
CDS	210..4373
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVEDRFRKTNHSSPEAQAKQTGRGLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008767003.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767003.2

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG 
GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT 
TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG 
GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT 
TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT 
CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG 
CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG 
AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG 
CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA 
TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC 
AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGA CTTGACTGCG 
TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTGCGGCCAC CTCACAAAGT GACAGACTAT 
TCCTCCTCCA GTGAGGAGTC TGGGACCACG GATGAGGAGG AGGAGGATGT GGAACAGGAG 
GGGGCTGATG ATTCCACCTC GGGGCCAGAG GACACCAGAG CAGCGTCGTC TCTGAATCTG 
AGCAACGGTG AGACAGAATC TGTGAAAACA ATGATTGTTC ATGATGACGT AGAAAGTGAA 
CCAGCCATGA CCCCATCCAA GGAGGGCACC CTAATCGTCC GCCAGAGTAC AGTTGACCAA 
AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCGGGTC GCATTCACCT CTTGCCAGAT 
CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CTTCCTCCCC ATCCTCCAGC 
CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT 
CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
GACCCCAGGT TACTACAGAT CTCTCCATCT AGTGGAACAA CAGTTACTTC CGTTGTGGGA 
TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC ATAAGGCAAG ATCCTACTCG GAAAGGCTCA 
GTGGTCAATG TGAACCCCAC GAACACTAGG CCACAGAGTG ATACCCCCGA GATTCGTAAA 
TACAAGAAGA GATTTAACTC AGAGATCCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA 
GTTGGCACAG AGAGTGGCCT GATGCTCTTG GACAGAAGTG GCCAAGGGAA AGTGTACCCT 
CTGATCAGCC GGAGACGGTT CCAGCAAATG GATGTACTTG AGGGCCTGAA TGTCCTGGTG 
ACAATATCTG GCAAAAAGGA TAAGTTACGC GTCTACTATT TATCCTGGTT AAGAAATAAA 
ATACTTCATA ATGATCCGGA AGTTGAGAAG AAGCAGGGGT GGACCACCGT GGGCGACCTG 
GAAGGGTGCG TGCACTATAA AGTCGTAAAA TATGAAAGAA TCAAGTTTCT GGTAATCGCT 
TTGAAGAGTT CTGTGGAAGT CTATGCATGG GCACCGAAGC CATATCACAA ATTTATGGCC 
TTTAAGTCAT TTGGAGAACT GGTACATAAG CCATTACTGG TGGATCTCAC TGTCGAGGAA 
GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT 
TCCGGCTCAG TCTATGACAT TTATCTACCA ACACACGTAA GAAAGAACCC ACACTCTATG 
ATTCAGTGTA CCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGAATGGAG 
CTTCTGGTGT GCTACGAAGA TGAAGGGGTT TATGTGAACA CGTACGGAAG AATCACCAAG 
GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATACATCCG GTCGAATCAG 
ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGCTCTG TGGAAACTGG TCATTTGGAC 
GGTGTGTTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG TAATGACAAG 
GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC AGCAGCCAGG TCTACTTCAT GACCTTAGGC 
AGGACTTCCC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_008767004.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765226.2 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa62694
Clone ID Related Accession (Same CDS sequence)	XM_008767004.2
Accession Version	XM_008767004.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767004.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG 
GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT 
TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG 
GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT 
TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT 
CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG 
CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG 
AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG 
CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA 
TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC 
AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGG AGAAGTGGAC 
TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG 
ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG 
GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT 
CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA 
GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGAGTACA 
GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC 
TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC TTCCTCCCCA 
TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT 
AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
CCTTTTATAG ACCCCAGGTT ACTACAGATC TCTCCATCTA GTGGAACAAC AGTTACTTCC 
GTTGTGGGAT TTTCCTGTGA TGGGCTGAGA CCAGAAGCCA TAAGGCAAGA TCCTACTCGG 
AAAGGCTCAG TGGTCAATGT GAACCCCACG AACACTAGGC CACAGAGTGA TACCCCCGAG 
ATTCGTAAAT ACAAGAAGAG ATTTAACTCA GAGATCCTGT GTGCTGCCTT ATGGGGAGTG 
AATTTGCTAG TTGGCACAGA GAGTGGCCTG ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA 
GTGTACCCTC TGATCAGCCG GAGACGGTTC CAGCAAATGG ATGTACTTGA GGGCCTGAAT 
GTCCTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGCG TCTACTATTT ATCCTGGTTA 
AGAAATAAAA TACTTCATAA TGATCCGGAA GTTGAGAAGA AGCAGGGGTG GACCACCGTG 
GGCGACCTGG AAGGGTGCGT GCACTATAAA GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG 
GTAATCGCTT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CACCGAAGCC ATATCACAAA 
TTTATGGCCT TTAAGTCATT TGGAGAACTG GTACATAAGC CATTACTGGT GGATCTCACT 
GTCGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
GATGTGGATT CCGGCTCAGT CTATGACATT TATCTACCAA CACACATTCA GTGTACCATC 
AAACCCCATG CAATCATTAT CCTCCCCAAC ACGGATGGAA TGGAGCTTCT GGTGTGCTAC 
GAAGATGAAG GGGTTTATGT GAACACGTAC GGAAGAATCA CCAAGGACGT GGTTCTGCAG 
TGGGGAGAGA TGCCAACATC TGTAGCATAC ATCCGGTCGA ATCAGACAAT GGGCTGGGGA 
GAGAAGGCAA TAGAGATACG CTCTGTGGAA ACTGGTCATT TGGACGGTGT GTTTATGCAC 
AAAAGGGCTC AGAGACTAAA GTTCCTGTGT GAACGTAATG ACAAGGTGTT CTTTGCTTCC 
GTCCGCTCTG GCGGCAGCAG CCAGGTCTAC TTCATGACCT TAGGCAGGAC TTCCCTTCTG 
AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765226.2
CDS	210..4358
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVEDRFRKTNHSSPEAQAKQTGRGLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008767004.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767004.2

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG 
GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT 
TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG 
GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT 
TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT 
CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG 
CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG 
AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG 
CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA 
TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC 
AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGG AGAAGTGGAC 
TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG 
ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG 
GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT 
CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA 
GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGAGTACA 
GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC 
TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC TTCCTCCCCA 
TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT 
AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
CCTTTTATAG ACCCCAGGTT ACTACAGATC TCTCCATCTA GTGGAACAAC AGTTACTTCC 
GTTGTGGGAT TTTCCTGTGA TGGGCTGAGA CCAGAAGCCA TAAGGCAAGA TCCTACTCGG 
AAAGGCTCAG TGGTCAATGT GAACCCCACG AACACTAGGC CACAGAGTGA TACCCCCGAG 
ATTCGTAAAT ACAAGAAGAG ATTTAACTCA GAGATCCTGT GTGCTGCCTT ATGGGGAGTG 
AATTTGCTAG TTGGCACAGA GAGTGGCCTG ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA 
GTGTACCCTC TGATCAGCCG GAGACGGTTC CAGCAAATGG ATGTACTTGA GGGCCTGAAT 
GTCCTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGCG TCTACTATTT ATCCTGGTTA 
AGAAATAAAA TACTTCATAA TGATCCGGAA GTTGAGAAGA AGCAGGGGTG GACCACCGTG 
GGCGACCTGG AAGGGTGCGT GCACTATAAA GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG 
GTAATCGCTT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CACCGAAGCC ATATCACAAA 
TTTATGGCCT TTAAGTCATT TGGAGAACTG GTACATAAGC CATTACTGGT GGATCTCACT 
GTCGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
GATGTGGATT CCGGCTCAGT CTATGACATT TATCTACCAA CACACATTCA GTGTACCATC 
AAACCCCATG CAATCATTAT CCTCCCCAAC ACGGATGGAA TGGAGCTTCT GGTGTGCTAC 
GAAGATGAAG GGGTTTATGT GAACACGTAC GGAAGAATCA CCAAGGACGT GGTTCTGCAG 
TGGGGAGAGA TGCCAACATC TGTAGCATAC ATCCGGTCGA ATCAGACAAT GGGCTGGGGA 
GAGAAGGCAA TAGAGATACG CTCTGTGGAA ACTGGTCATT TGGACGGTGT GTTTATGCAC 
AAAAGGGCTC AGAGACTAAA GTTCCTGTGT GAACGTAATG ACAAGGTGTT CTTTGCTTCC 
GTCCGCTCTG GCGGCAGCAG CCAGGTCTAC TTCATGACCT TAGGCAGGAC TTCCCTTCTG 
AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_008767005.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765227.2 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa62695
Clone ID Related Accession (Same CDS sequence)	XM_008767005.2
Accession Version	XM_008767005.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767005.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT 
AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG 
CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT 
GCCCTGCAGA GACCAGCGGA GCCACAGGTA CAGTGGTCCC ACCTGGCATC TCTCAAGAAC 
AATGTCTCCC CTGTCTCGAG ATCCCATTCC TTCAGTGACC CTTCTCCCAA ATTCGCACAC 
CACCATCTCC GCTCTCAGGA CCCATGTCCA CCTTCCCGCA GTGAGGGGCT CAGTCAGAGC 
TCTGACTCTA AGTCGGAGGT ACCTGAGCCC ACCCAAAAGG CTTGGTCTAG ATCAGACAGT 
GACGAGGTGC CTCCAAGGGT TCCTGTGAGA ACGACATCTC GTTCCCCTGT CCTGTCCCGT 
CGAGATTCTC CACTGCAGGG CAGTGGGCAG CAAAATAGCC AAGCAGGACA GAGAAATTCC 
ACCAGCAGTA TTGAGCCCCG GCTTCTATGG GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA 
GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC TCAGGATCCC AGCCTGGCTC CCATCCTGGG 
TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA GTGAGATCAT CATCCAAGTC TGAAGGTTCT 
CCATCTCAGC GCCTTGAAAA TGCAGCCAAA AAACCTGAAG ATAAGAAAGA AGTATTTCGA 
CCTCTCAAAC CTGCTGGAGA AGTGGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG 
GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG 
ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG 
CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG 
AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG 
GGCACCCTAA TCGTCCGCCA GAGTACAGTT GACCAAAAGC GTGCCAGCCA TCATGAGAGC 
AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
TCCACTTCCT CCACCTCTTC CTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
CAGACACCCC AGGACAAGCT CACTGCTAAT GAGACTCAGT CCGCTAGTAG CACACTCCAG 
AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGGTTACT ACAGATCTCT 
CCATCTAGTG GAACAACAGT TACTTCCGTT GTGGGATTTT CCTGTGATGG GCTGAGACCA 
GAAGCCATAA GGCAAGATCC TACTCGGAAA GGCTCAGTGG TCAATGTGAA CCCCACGAAC 
ACTAGGCCAC AGAGTGATAC CCCCGAGATT CGTAAATACA AGAAGAGATT TAACTCAGAG 
ATCCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTTG GCACAGAGAG TGGCCTGATG 
CTCTTGGACA GAAGTGGCCA AGGGAAAGTG TACCCTCTGA TCAGCCGGAG ACGGTTCCAG 
CAAATGGATG TACTTGAGGG CCTGAATGTC CTGGTGACAA TATCTGGCAA AAAGGATAAG 
TTACGCGTCT ACTATTTATC CTGGTTAAGA AATAAAATAC TTCATAATGA TCCGGAAGTT 
GAGAAGAAGC AGGGGTGGAC CACCGTGGGC GACCTGGAAG GGTGCGTGCA CTATAAAGTC 
GTAAAATATG AAAGAATCAA GTTTCTGGTA ATCGCTTTGA AGAGTTCTGT GGAAGTCTAT 
GCATGGGCAC CGAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAACTGGTA 
CATAAGCCAT TACTGGTGGA TCTCACTGTC GAGGAAGGCC AGAGGTTGAA AGTGATCTAT 
GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCCG GCTCAGTCTA TGACATTTAT 
CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATTC AGTGTACCAT CAAACCCCAT 
GCAATCATTA TCCTCCCCAA CACGGATGGA ATGGAGCTTC TGGTGTGCTA CGAAGATGAA 
GGGGTTTATG TGAACACGTA CGGAAGAATC ACCAAGGACG TGGTTCTGCA GTGGGGAGAG 
ATGCCAACAT CTGTAGCATA CATCCGGTCG AATCAGACAA TGGGCTGGGG AGAGAAGGCA 
ATAGAGATAC GCTCTGTGGA AACTGGTCAT TTGGACGGTG TGTTTATGCA CAAAAGGGCT 
CAGAGACTAA AGTTCCTGTG TGAACGTAAT GACAAGGTGT TCTTTGCTTC CGTCCGCTCT 
GGCGGCAGCA GCCAGGTCTA CTTCATGACC TTAGGCAGGA CTTCCCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765227.2
CDS	211..4290
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVEDRFRKTNHSSPEAQAKQTGRGLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008767005.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767005.2

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT 
AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG 
CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT 
GCCCTGCAGA GACCAGCGGA GCCACAGGTA CAGTGGTCCC ACCTGGCATC TCTCAAGAAC 
AATGTCTCCC CTGTCTCGAG ATCCCATTCC TTCAGTGACC CTTCTCCCAA ATTCGCACAC 
CACCATCTCC GCTCTCAGGA CCCATGTCCA CCTTCCCGCA GTGAGGGGCT CAGTCAGAGC 
TCTGACTCTA AGTCGGAGGT ACCTGAGCCC ACCCAAAAGG CTTGGTCTAG ATCAGACAGT 
GACGAGGTGC CTCCAAGGGT TCCTGTGAGA ACGACATCTC GTTCCCCTGT CCTGTCCCGT 
CGAGATTCTC CACTGCAGGG CAGTGGGCAG CAAAATAGCC AAGCAGGACA GAGAAATTCC 
ACCAGCAGTA TTGAGCCCCG GCTTCTATGG GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA 
GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC TCAGGATCCC AGCCTGGCTC CCATCCTGGG 
TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA GTGAGATCAT CATCCAAGTC TGAAGGTTCT 
CCATCTCAGC GCCTTGAAAA TGCAGCCAAA AAACCTGAAG ATAAGAAAGA AGTATTTCGA 
CCTCTCAAAC CTGCTGGAGA AGTGGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG 
GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG 
ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG 
CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG 
AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG 
GGCACCCTAA TCGTCCGCCA GAGTACAGTT GACCAAAAGC GTGCCAGCCA TCATGAGAGC 
AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
TCCACTTCCT CCACCTCTTC CTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
CAGACACCCC AGGACAAGCT CACTGCTAAT GAGACTCAGT CCGCTAGTAG CACACTCCAG 
AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGGTTACT ACAGATCTCT 
CCATCTAGTG GAACAACAGT TACTTCCGTT GTGGGATTTT CCTGTGATGG GCTGAGACCA 
GAAGCCATAA GGCAAGATCC TACTCGGAAA GGCTCAGTGG TCAATGTGAA CCCCACGAAC 
ACTAGGCCAC AGAGTGATAC CCCCGAGATT CGTAAATACA AGAAGAGATT TAACTCAGAG 
ATCCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTTG GCACAGAGAG TGGCCTGATG 
CTCTTGGACA GAAGTGGCCA AGGGAAAGTG TACCCTCTGA TCAGCCGGAG ACGGTTCCAG 
CAAATGGATG TACTTGAGGG CCTGAATGTC CTGGTGACAA TATCTGGCAA AAAGGATAAG 
TTACGCGTCT ACTATTTATC CTGGTTAAGA AATAAAATAC TTCATAATGA TCCGGAAGTT 
GAGAAGAAGC AGGGGTGGAC CACCGTGGGC GACCTGGAAG GGTGCGTGCA CTATAAAGTC 
GTAAAATATG AAAGAATCAA GTTTCTGGTA ATCGCTTTGA AGAGTTCTGT GGAAGTCTAT 
GCATGGGCAC CGAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAACTGGTA 
CATAAGCCAT TACTGGTGGA TCTCACTGTC GAGGAAGGCC AGAGGTTGAA AGTGATCTAT 
GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCCG GCTCAGTCTA TGACATTTAT 
CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATTC AGTGTACCAT CAAACCCCAT 
GCAATCATTA TCCTCCCCAA CACGGATGGA ATGGAGCTTC TGGTGTGCTA CGAAGATGAA 
GGGGTTTATG TGAACACGTA CGGAAGAATC ACCAAGGACG TGGTTCTGCA GTGGGGAGAG 
ATGCCAACAT CTGTAGCATA CATCCGGTCG AATCAGACAA TGGGCTGGGG AGAGAAGGCA 
ATAGAGATAC GCTCTGTGGA AACTGGTCAT TTGGACGGTG TGTTTATGCA CAAAAGGGCT 
CAGAGACTAA AGTTCCTGTG TGAACGTAAT GACAAGGTGT TCTTTGCTTC CGTCCGCTCT 
GGCGGCAGCA GCCAGGTCTA CTTCATGACC TTAGGCAGGA CTTCCCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_008767006.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765228.2 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ORa62696
Clone ID Related Accession (Same CDS sequence)	XM_008767006.2
Accession Version	XM_008767006.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT ACAGTGGTCC CACCTGGCAT CTCTCAAGAA CAATGTCTCC 
CCTGTCTCGA GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTCGCACA CCACCATCTC 
CGCTCTCAGG ACCCATGTCC ACCTTCCCGC AGTGAGGGGC TCAGTCAGAG CTCTGACTCT 
AAGTCGGAGG TACCTGAGCC CACCCAAAAG GCTTGGTCTA GATCAGACAG TGACGAGGTG 
CCTCCAAGGG TTCCTGTGAG AACGACATCT CGTTCCCCTG TCCTGTCCCG TCGAGATTCT 
CCACTGCAGG GCAGTGGGCA GCAAAATAGC CAAGCAGGAC AGAGAAATTC CACCAGCAGT 
ATTGAGCCCC GGCTTCTATG GGAGAGAGTG GAAAAGCTGG TCCCCAGGCC AGGCAGTGGC 
AGCTCTTCAG GGTCCAGCAA CTCAGGATCC CAGCCTGGCT CCCATCCTGG GTCTCAGAGT 
GGCTCTGGTG AACGCTTCAG AGTGAGATCA TCATCCAAGT CTGAAGGTTC TCCATCTCAG 
CGCCTTGAAA ATGCAGCCAA AAAACCTGAA GATAAGAAAG AAGTATTTCG ACCTCTCAAA 
CCTGCTGGAG AAGTGGACTT GACTGCGTTG GCCAAAGAGC TTCGAGCAGT GGAAGATGTG 
CGGCCACCTC ACAAAGTGAC AGACTATTCC TCCTCCAGTG AGGAGTCTGG GACCACGGAT 
GAGGAGGAGG AGGATGTGGA ACAGGAGGGG GCTGATGATT CCACCTCGGG GCCAGAGGAC 
ACCAGAGCAG CGTCGTCTCT GAATCTGAGC AACGGTGAGA CAGAATCTGT GAAAACAATG 
ATTGTTCATG ATGACGTAGA AAGTGAACCA GCCATGACCC CATCCAAGGA GGGCACCCTA 
ATCGTCCGCC AGAGTACAGT TGACCAAAAG CGTGCCAGCC ATCATGAGAG CAATGGCTTT 
GCGGGTCGCA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACTTCC 
TCCACCTCTT CCTCCCCATC CTCCAGCCAG CCGACACCCA CCATGTCCCC ACAGACACCC 
CAGGACAAGC TCACTGCTAA TGAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA 
TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGGTTAC TACAGATCTC TCCATCTAGT 
GGAACAACAG TTACTTCCGT TGTGGGATTT TCCTGTGATG GGCTGAGACC AGAAGCCATA 
AGGCAAGATC CTACTCGGAA AGGCTCAGTG GTCAATGTGA ACCCCACGAA CACTAGGCCA 
CAGAGTGATA CCCCCGAGAT TCGTAAATAC AAGAAGAGAT TTAACTCAGA GATCCTGTGT 
GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT GGCACAGAGA GTGGCCTGAT GCTCTTGGAC 
AGAAGTGGCC AAGGGAAAGT GTACCCTCTG ATCAGCCGGA GACGGTTCCA GCAAATGGAT 
GTACTTGAGG GCCTGAATGT CCTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGCGTC 
TACTATTTAT CCTGGTTAAG AAATAAAATA CTTCATAATG ATCCGGAAGT TGAGAAGAAG 
CAGGGGTGGA CCACCGTGGG CGACCTGGAA GGGTGCGTGC ACTATAAAGT CGTAAAATAT 
GAAAGAATCA AGTTTCTGGT AATCGCTTTG AAGAGTTCTG TGGAAGTCTA TGCATGGGCA 
CCGAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAACTGGT ACATAAGCCA 
TTACTGGTGG ATCTCACTGT CGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT 
GCTGGATTCC ATGCTGTTGA TGTGGATTCC GGCTCAGTCT ATGACATTTA TCTACCAACA 
CACGTAAGAA AGAACCCACA CTCTATGATT CAGTGTACCA TCAAACCCCA TGCAATCATT 
ATCCTCCCCA ACACGGATGG AATGGAGCTT CTGGTGTGCT ACGAAGATGA AGGGGTTTAT 
GTGAACACGT ACGGAAGAAT CACCAAGGAC GTGGTTCTGC AGTGGGGAGA GATGCCAACA 
TCTGTAGCAT ACATCCGGTC GAATCAGACA ATGGGCTGGG GAGAGAAGGC AATAGAGATA 
CGCTCTGTGG AAACTGGTCA TTTGGACGGT GTGTTTATGC ACAAAAGGGC TCAGAGACTA 
AAGTTCCTGT GTGAACGTAA TGACAAGGTG TTCTTTGCTT CCGTCCGCTC TGGCGGCAGC 
AGCCAGGTCT ACTTCATGAC CTTAGGCAGG ACTTCCCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765228.2
CDS	210..4220
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008767006.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767006.2

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT ACAGTGGTCC CACCTGGCAT CTCTCAAGAA CAATGTCTCC 
CCTGTCTCGA GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTCGCACA CCACCATCTC 
CGCTCTCAGG ACCCATGTCC ACCTTCCCGC AGTGAGGGGC TCAGTCAGAG CTCTGACTCT 
AAGTCGGAGG TACCTGAGCC CACCCAAAAG GCTTGGTCTA GATCAGACAG TGACGAGGTG 
CCTCCAAGGG TTCCTGTGAG AACGACATCT CGTTCCCCTG TCCTGTCCCG TCGAGATTCT 
CCACTGCAGG GCAGTGGGCA GCAAAATAGC CAAGCAGGAC AGAGAAATTC CACCAGCAGT 
ATTGAGCCCC GGCTTCTATG GGAGAGAGTG GAAAAGCTGG TCCCCAGGCC AGGCAGTGGC 
AGCTCTTCAG GGTCCAGCAA CTCAGGATCC CAGCCTGGCT CCCATCCTGG GTCTCAGAGT 
GGCTCTGGTG AACGCTTCAG AGTGAGATCA TCATCCAAGT CTGAAGGTTC TCCATCTCAG 
CGCCTTGAAA ATGCAGCCAA AAAACCTGAA GATAAGAAAG AAGTATTTCG ACCTCTCAAA 
CCTGCTGGAG AAGTGGACTT GACTGCGTTG GCCAAAGAGC TTCGAGCAGT GGAAGATGTG 
CGGCCACCTC ACAAAGTGAC AGACTATTCC TCCTCCAGTG AGGAGTCTGG GACCACGGAT 
GAGGAGGAGG AGGATGTGGA ACAGGAGGGG GCTGATGATT CCACCTCGGG GCCAGAGGAC 
ACCAGAGCAG CGTCGTCTCT GAATCTGAGC AACGGTGAGA CAGAATCTGT GAAAACAATG 
ATTGTTCATG ATGACGTAGA AAGTGAACCA GCCATGACCC CATCCAAGGA GGGCACCCTA 
ATCGTCCGCC AGAGTACAGT TGACCAAAAG CGTGCCAGCC ATCATGAGAG CAATGGCTTT 
GCGGGTCGCA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACTTCC 
TCCACCTCTT CCTCCCCATC CTCCAGCCAG CCGACACCCA CCATGTCCCC ACAGACACCC 
CAGGACAAGC TCACTGCTAA TGAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA 
TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGGTTAC TACAGATCTC TCCATCTAGT 
GGAACAACAG TTACTTCCGT TGTGGGATTT TCCTGTGATG GGCTGAGACC AGAAGCCATA 
AGGCAAGATC CTACTCGGAA AGGCTCAGTG GTCAATGTGA ACCCCACGAA CACTAGGCCA 
CAGAGTGATA CCCCCGAGAT TCGTAAATAC AAGAAGAGAT TTAACTCAGA GATCCTGTGT 
GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT GGCACAGAGA GTGGCCTGAT GCTCTTGGAC 
AGAAGTGGCC AAGGGAAAGT GTACCCTCTG ATCAGCCGGA GACGGTTCCA GCAAATGGAT 
GTACTTGAGG GCCTGAATGT CCTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGCGTC 
TACTATTTAT CCTGGTTAAG AAATAAAATA CTTCATAATG ATCCGGAAGT TGAGAAGAAG 
CAGGGGTGGA CCACCGTGGG CGACCTGGAA GGGTGCGTGC ACTATAAAGT CGTAAAATAT 
GAAAGAATCA AGTTTCTGGT AATCGCTTTG AAGAGTTCTG TGGAAGTCTA TGCATGGGCA 
CCGAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAACTGGT ACATAAGCCA 
TTACTGGTGG ATCTCACTGT CGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT 
GCTGGATTCC ATGCTGTTGA TGTGGATTCC GGCTCAGTCT ATGACATTTA TCTACCAACA 
CACGTAAGAA AGAACCCACA CTCTATGATT CAGTGTACCA TCAAACCCCA TGCAATCATT 
ATCCTCCCCA ACACGGATGG AATGGAGCTT CTGGTGTGCT ACGAAGATGA AGGGGTTTAT 
GTGAACACGT ACGGAAGAAT CACCAAGGAC GTGGTTCTGC AGTGGGGAGA GATGCCAACA 
TCTGTAGCAT ACATCCGGTC GAATCAGACA ATGGGCTGGG GAGAGAAGGC AATAGAGATA 
CGCTCTGTGG AAACTGGTCA TTTGGACGGT GTGTTTATGC ACAAAAGGGC TCAGAGACTA 
AAGTTCCTGT GTGAACGTAA TGACAAGGTG TTCTTTGCTT CCGTCCGCTC TGGCGGCAGC 
AGCCAGGTCT ACTTCATGAC CTTAGGCAGG ACTTCCCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_017596340.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451829.1 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ORa62697
Clone ID Related Accession (Same CDS sequence)	XM_017596340.1
Accession Version	XM_017596340.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT CTCCCCTGTC 
TCGAGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTCG CACACCACCA TCTCCGCTCT 
CAGGACCCAT GTCCACCTTC CCGCAGTGAG GGGCTCAGTC AGAGCTCTGA CTCTAAGTCG 
GAGGTACCTG AGCCCACCCA AAAGGCTTGG TCTAGATCAG ACAGTGACGA GGTGCCTCCA 
AGGGTTCCTG TGAGAACGAC ATCTCGTTCC CCTGTCCTGT CCCGTCGAGA TTCTCCACTG 
CAGGGCAGTG GGCAGCAAAA TAGCCAAGCA GGACAGAGAA ATTCCACCAG CAGTATTGAG 
CCCCGGCTTC TATGGGAGAG AGTGGAAAAG CTGGTCCCCA GGCCAGGCAG TGGCAGCTCT 
TCAGGGTCCA GCAACTCAGG ATCCCAGCCT GGCTCCCATC CTGGGTCTCA GAGTGGCTCT 
GGTGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GTTCTCCATC TCAGCGCCTT 
GAAAATGCAG CCAAAAAACC TGAAGATAAG AAAGAAGTAT TTCGACCTCT CAAACCTGCT 
GGAGAAGTGG ACTTGACTGC GTTGGCCAAA GAGCTTCGAG CAGTGGAAGA TGTGCGGCCA 
CCTCACAAAG TGACAGACTA TTCCTCCTCC AGTGAGGAGT CTGGGACCAC GGATGAGGAG 
GAGGAGGATG TGGAACAGGA GGGGGCTGAT GATTCCACCT CGGGGCCAGA GGACACCAGA 
GCAGCGTCGT CTCTGAATCT GAGCAACGGT GAGACAGAAT CTGTGAAAAC AATGATTGTT 
CATGATGACG TAGAAAGTGA ACCAGCCATG ACCCCATCCA AGGAGGGCAC CCTAATCGTC 
CGCCAGAGTA CAGTTGACCA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCGGGT 
CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC 
TCTTCCTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC 
AAGCTCACTG CTAATGAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC 
TCCTCCTTTA CACCTTTTAT AGACCCCAGG TTACTACAGA TCTCTCCATC TAGTGGAACA 
ACAGTTACTT CCGTTGTGGG ATTTTCCTGT GATGGGCTGA GACCAGAAGC CATAAGGCAA 
GATCCTACTC GGAAAGGCTC AGTGGTCAAT GTGAACCCCA CGAACACTAG GCCACAGAGT 
GATACCCCCG AGATTCGTAA ATACAAGAAG AGATTTAACT CAGAGATCCT GTGTGCTGCC 
TTATGGGGAG TGAATTTGCT AGTTGGCACA GAGAGTGGCC TGATGCTCTT GGACAGAAGT 
GGCCAAGGGA AAGTGTACCC TCTGATCAGC CGGAGACGGT TCCAGCAAAT GGATGTACTT 
GAGGGCCTGA ATGTCCTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG CGTCTACTAT 
TTATCCTGGT TAAGAAATAA AATACTTCAT AATGATCCGG AAGTTGAGAA GAAGCAGGGG 
TGGACCACCG TGGGCGACCT GGAAGGGTGC GTGCACTATA AAGTCGTAAA ATATGAAAGA 
ATCAAGTTTC TGGTAATCGC TTTGAAGAGT TCTGTGGAAG TCTATGCATG GGCACCGAAG 
CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAC TGGTACATAA GCCATTACTG 
GTGGATCTCA CTGTCGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA 
TTCCATGCTG TTGATGTGGA TTCCGGCTCA GTCTATGACA TTTATCTACC AACACACGTA 
AGAAAGAACC CACACTCTAT GATTCAGTGT ACCATCAAAC CCCATGCAAT CATTATCCTC 
CCCAACACGG ATGGAATGGA GCTTCTGGTG TGCTACGAAG ATGAAGGGGT TTATGTGAAC 
ACGTACGGAA GAATCACCAA GGACGTGGTT CTGCAGTGGG GAGAGATGCC AACATCTGTA 
GCATACATCC GGTCGAATCA GACAATGGGC TGGGGAGAGA AGGCAATAGA GATACGCTCT 
GTGGAAACTG GTCATTTGGA CGGTGTGTTT ATGCACAAAA GGGCTCAGAG ACTAAAGTTC 
CTGTGTGAAC GTAATGACAA GGTGTTCTTT GCTTCCGTCC GCTCTGGCGG CAGCAGCCAG 
GTCTACTTCA TGACCTTAGG CAGGACTTCC CTTCTGAGCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451829.1
CDS	210..4214
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017596340.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596340.1

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT CTCCCCTGTC 
TCGAGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTCG CACACCACCA TCTCCGCTCT 
CAGGACCCAT GTCCACCTTC CCGCAGTGAG GGGCTCAGTC AGAGCTCTGA CTCTAAGTCG 
GAGGTACCTG AGCCCACCCA AAAGGCTTGG TCTAGATCAG ACAGTGACGA GGTGCCTCCA 
AGGGTTCCTG TGAGAACGAC ATCTCGTTCC CCTGTCCTGT CCCGTCGAGA TTCTCCACTG 
CAGGGCAGTG GGCAGCAAAA TAGCCAAGCA GGACAGAGAA ATTCCACCAG CAGTATTGAG 
CCCCGGCTTC TATGGGAGAG AGTGGAAAAG CTGGTCCCCA GGCCAGGCAG TGGCAGCTCT 
TCAGGGTCCA GCAACTCAGG ATCCCAGCCT GGCTCCCATC CTGGGTCTCA GAGTGGCTCT 
GGTGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GTTCTCCATC TCAGCGCCTT 
GAAAATGCAG CCAAAAAACC TGAAGATAAG AAAGAAGTAT TTCGACCTCT CAAACCTGCT 
GGAGAAGTGG ACTTGACTGC GTTGGCCAAA GAGCTTCGAG CAGTGGAAGA TGTGCGGCCA 
CCTCACAAAG TGACAGACTA TTCCTCCTCC AGTGAGGAGT CTGGGACCAC GGATGAGGAG 
GAGGAGGATG TGGAACAGGA GGGGGCTGAT GATTCCACCT CGGGGCCAGA GGACACCAGA 
GCAGCGTCGT CTCTGAATCT GAGCAACGGT GAGACAGAAT CTGTGAAAAC AATGATTGTT 
CATGATGACG TAGAAAGTGA ACCAGCCATG ACCCCATCCA AGGAGGGCAC CCTAATCGTC 
CGCCAGAGTA CAGTTGACCA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCGGGT 
CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC 
TCTTCCTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC 
AAGCTCACTG CTAATGAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC 
TCCTCCTTTA CACCTTTTAT AGACCCCAGG TTACTACAGA TCTCTCCATC TAGTGGAACA 
ACAGTTACTT CCGTTGTGGG ATTTTCCTGT GATGGGCTGA GACCAGAAGC CATAAGGCAA 
GATCCTACTC GGAAAGGCTC AGTGGTCAAT GTGAACCCCA CGAACACTAG GCCACAGAGT 
GATACCCCCG AGATTCGTAA ATACAAGAAG AGATTTAACT CAGAGATCCT GTGTGCTGCC 
TTATGGGGAG TGAATTTGCT AGTTGGCACA GAGAGTGGCC TGATGCTCTT GGACAGAAGT 
GGCCAAGGGA AAGTGTACCC TCTGATCAGC CGGAGACGGT TCCAGCAAAT GGATGTACTT 
GAGGGCCTGA ATGTCCTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG CGTCTACTAT 
TTATCCTGGT TAAGAAATAA AATACTTCAT AATGATCCGG AAGTTGAGAA GAAGCAGGGG 
TGGACCACCG TGGGCGACCT GGAAGGGTGC GTGCACTATA AAGTCGTAAA ATATGAAAGA 
ATCAAGTTTC TGGTAATCGC TTTGAAGAGT TCTGTGGAAG TCTATGCATG GGCACCGAAG 
CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAC TGGTACATAA GCCATTACTG 
GTGGATCTCA CTGTCGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA 
TTCCATGCTG TTGATGTGGA TTCCGGCTCA GTCTATGACA TTTATCTACC AACACACGTA 
AGAAAGAACC CACACTCTAT GATTCAGTGT ACCATCAAAC CCCATGCAAT CATTATCCTC 
CCCAACACGG ATGGAATGGA GCTTCTGGTG TGCTACGAAG ATGAAGGGGT TTATGTGAAC 
ACGTACGGAA GAATCACCAA GGACGTGGTT CTGCAGTGGG GAGAGATGCC AACATCTGTA 
GCATACATCC GGTCGAATCA GACAATGGGC TGGGGAGAGA AGGCAATAGA GATACGCTCT 
GTGGAAACTG GTCATTTGGA CGGTGTGTTT ATGCACAAAA GGGCTCAGAG ACTAAAGTTC 
CTGTGTGAAC GTAATGACAA GGTGTTCTTT GCTTCCGTCC GCTCTGGCGG CAGCAGCCAG 
GTCTACTTCA TGACCTTAGG CAGGACTTCC CTTCTGAGCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_008767007.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765229.2 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ORa62698
Clone ID Related Accession (Same CDS sequence)	XM_008767007.2
Accession Version	XM_008767007.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767007.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG 
GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT 
TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG 
GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT 
TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT 
CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG 
CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG 
AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG 
CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA 
TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC 
AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGG AGAAGTGGAC 
TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG 
ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG 
GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT 
CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA 
GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGACTCAG 
TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
CCCAGGTTAC TACAGATCTC TCCATCTAGT GGAACAACAG TTACTTCCGT TGTGGGATTT 
TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG 
GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC 
AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT 
GGCACAGAGA GTGGCCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT GTACCCTCTG 
ATCAGCCGGA GACGGTTCCA GCAAATGGAT GTACTTGAGG GCCTGAATGT CCTGGTGACA 
ATATCTGGCA AAAAGGATAA GTTACGCGTC TACTATTTAT CCTGGTTAAG AAATAAAATA 
CTTCATAATG ATCCGGAAGT TGAGAAGAAG CAGGGGTGGA CCACCGTGGG CGACCTGGAA 
GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATCGCTTTG 
AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT 
AAGTCATTTG GAGAACTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT CGAGGAAGGC 
CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCC 
GGCTCAGTCT ATGACATTTA TCTACCAACA CACGTAAGAA AGAACCCACA CTCTATGATT 
CAGTGTACCA TCAAACCCCA TGCAATCATT ATCCTCCCCA ACACGGATGG AATGGAGCTT 
CTGGTGTGCT ACGAAGATGA AGGGGTTTAT GTGAACACGT ACGGAAGAAT CACCAAGGAC 
GTGGTTCTGC AGTGGGGAGA GATGCCAACA TCTGTAGCAT ACATCCGGTC GAATCAGACA 
ATGGGCTGGG GAGAGAAGGC AATAGAGATA CGCTCTGTGG AAACTGGTCA TTTGGACGGT 
GTGTTTATGC ACAAAAGGGC TCAGAGACTA AAGTTCCTGT GTGAACGTAA TGACAAGGTG 
TTCTTTGCTT CCGTCCGCTC TGGCGGCAGC AGCCAGGTCT ACTTCATGAC CTTAGGCAGG 
ACTTCCCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765229.2
CDS	211..4191
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVEDRFRKTNHSSPEAQAKQTGRGLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008767007.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767007.2

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG 
GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT 
TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG 
GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT 
TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT 
CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG 
CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG 
AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG 
CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA 
TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC 
AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGG AGAAGTGGAC 
TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG 
ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG 
GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT 
CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA 
GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGACTCAG 
TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
CCCAGGTTAC TACAGATCTC TCCATCTAGT GGAACAACAG TTACTTCCGT TGTGGGATTT 
TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG 
GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC 
AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT 
GGCACAGAGA GTGGCCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT GTACCCTCTG 
ATCAGCCGGA GACGGTTCCA GCAAATGGAT GTACTTGAGG GCCTGAATGT CCTGGTGACA 
ATATCTGGCA AAAAGGATAA GTTACGCGTC TACTATTTAT CCTGGTTAAG AAATAAAATA 
CTTCATAATG ATCCGGAAGT TGAGAAGAAG CAGGGGTGGA CCACCGTGGG CGACCTGGAA 
GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATCGCTTTG 
AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT 
AAGTCATTTG GAGAACTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT CGAGGAAGGC 
CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCC 
GGCTCAGTCT ATGACATTTA TCTACCAACA CACGTAAGAA AGAACCCACA CTCTATGATT 
CAGTGTACCA TCAAACCCCA TGCAATCATT ATCCTCCCCA ACACGGATGG AATGGAGCTT 
CTGGTGTGCT ACGAAGATGA AGGGGTTTAT GTGAACACGT ACGGAAGAAT CACCAAGGAC 
GTGGTTCTGC AGTGGGGAGA GATGCCAACA TCTGTAGCAT ACATCCGGTC GAATCAGACA 
ATGGGCTGGG GAGAGAAGGC AATAGAGATA CGCTCTGTGG AAACTGGTCA TTTGGACGGT 
GTGTTTATGC ACAAAAGGGC TCAGAGACTA AAGTTCCTGT GTGAACGTAA TGACAAGGTG 
TTCTTTGCTT CCGTCCGCTC TGGCGGCAGC AGCCAGGTCT ACTTCATGAC CTTAGGCAGG 
ACTTCCCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_008767008.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765230.2 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ORa62699
Clone ID Related Accession (Same CDS sequence)	XM_008767008.2
Accession Version	XM_008767008.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG 
GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT 
TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG 
GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT 
TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT 
CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG 
CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG 
AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG 
CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA 
TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC 
AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGG AGAAGTGGAC 
TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG 
ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG 
GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT 
CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA 
GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGACTCAG 
TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
CCCAGGTTAC TACAGATCTC TCCATCTAGT GGAACAACAG TTACTTCCGT TGTGGGATTT 
TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG 
GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC 
AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT 
GGCACAGAGA GTGGCCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT GTACCCTCTG 
ATCAGCCGGA GACGGTTCCA GCAAATGGAT GTACTTGAGG GCCTGAATGT CCTGGTGACA 
ATATCTGGCA AAAAGGATAA GTTACGCGTC TACTATTTAT CCTGGTTAAG AAATAAAATA 
CTTCATAATG ATCCGGAAGT TGAGAAGAAG CAGGGGTGGA CCACCGTGGG CGACCTGGAA 
GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATCGCTTTG 
AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT 
AAGTCATTTG GAGAACTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT CGAGGAAGGC 
CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCC 
GGCTCAGTCT ATGACATTTA TCTACCAACA CACATTCAGT GTACCATCAA ACCCCATGCA 
ATCATTATCC TCCCCAACAC GGATGGAATG GAGCTTCTGG TGTGCTACGA AGATGAAGGG 
GTTTATGTGA ACACGTACGG AAGAATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG 
CCAACATCTG TAGCATACAT CCGGTCGAAT CAGACAATGG GCTGGGGAGA GAAGGCAATA 
GAGATACGCT CTGTGGAAAC TGGTCATTTG GACGGTGTGT TTATGCACAA AAGGGCTCAG 
AGACTAAAGT TCCTGTGTGA ACGTAATGAC AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC 
GGCAGCAGCC AGGTCTACTT CATGACCTTA GGCAGGACTT CCCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765230.2
CDS	210..4166
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVEDRFRKTNHSSPEAQAKQTGRGLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008767008.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767008.2

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG 
GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT 
TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG 
GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT 
TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT 
CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG 
CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG 
AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG 
CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA 
TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC 
AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGG AGAAGTGGAC 
TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG 
ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG 
GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT 
CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA 
GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGACTCAG 
TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
CCCAGGTTAC TACAGATCTC TCCATCTAGT GGAACAACAG TTACTTCCGT TGTGGGATTT 
TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG 
GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC 
AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT 
GGCACAGAGA GTGGCCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT GTACCCTCTG 
ATCAGCCGGA GACGGTTCCA GCAAATGGAT GTACTTGAGG GCCTGAATGT CCTGGTGACA 
ATATCTGGCA AAAAGGATAA GTTACGCGTC TACTATTTAT CCTGGTTAAG AAATAAAATA 
CTTCATAATG ATCCGGAAGT TGAGAAGAAG CAGGGGTGGA CCACCGTGGG CGACCTGGAA 
GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATCGCTTTG 
AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT 
AAGTCATTTG GAGAACTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT CGAGGAAGGC 
CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCC 
GGCTCAGTCT ATGACATTTA TCTACCAACA CACATTCAGT GTACCATCAA ACCCCATGCA 
ATCATTATCC TCCCCAACAC GGATGGAATG GAGCTTCTGG TGTGCTACGA AGATGAAGGG 
GTTTATGTGA ACACGTACGG AAGAATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG 
CCAACATCTG TAGCATACAT CCGGTCGAAT CAGACAATGG GCTGGGGAGA GAAGGCAATA 
GAGATACGCT CTGTGGAAAC TGGTCATTTG GACGGTGTGT TTATGCACAA AAGGGCTCAG 
AGACTAAAGT TCCTGTGTGA ACGTAATGAC AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC 
GGCAGCAGCC AGGTCTACTT CATGACCTTA GGCAGGACTT CCCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_008767009.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765231.2 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X11, mRNA.

Price & Availability↑

CloneID	ORa62700
Clone ID Related Accession (Same CDS sequence)	XM_008767009.2
Accession Version	XM_008767009.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG 
GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT 
TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG 
GTTCCTGTGA GAACGACATC TCGTTCCCCT GTCCTGTCCC GTCGAGATTC TCCACTGCAG 
GGCAGTGGGC AGCAAAATAG CCAAGCAGGA CAGAGAAATT CCACCAGCAG TATTGAGCCC 
CGGCTTCTAT GGGAGAGAGT GGAAAAGCTG GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA 
GGGTCCAGCA ACTCAGGATC CCAGCCTGGC TCCCATCCTG GGTCTCAGAG TGGCTCTGGT 
GAACGCTTCA GAGTGAGATC ATCATCCAAG TCTGAAGGTT CTCCATCTCA GCGCCTTGAA 
AATGCAGCCA AAAAACCTGA AGATAAGAAA GAAGTATTTC GACCTCTCAA ACCTGCTGGA 
GAAGTGGACT TGACTGCGTT GGCCAAAGAG CTTCGAGCAG TGGAAGATGT GCGGCCACCT 
CACAAAGTGA CAGACTATTC CTCCTCCAGT GAGGAGTCTG GGACCACGGA TGAGGAGGAG 
GAGGATGTGG AACAGGAGGG GGCTGATGAT TCCACCTCGG GGCCAGAGGA CACCAGAGCA 
GCGTCGTCTC TGAATCTGAG CAACGGTGAG ACAGAATCTG TGAAAACAAT GATTGTTCAT 
GATGACGTAG AAAGTGAACC AGCCATGACC CCATCCAAGG AGGGCACCCT AATCGTCCGC 
CAGAGTACAG TTGACCAAAA GCGTGCCAGC CATCATGAGA GCAATGGCTT TGCGGGTCGC 
ATTCACCTCT TGCCAGATCT CTTACAGCAA AGCCATTCCT CCTCCACTTC CTCCACCTCT 
TCCTCCCCAT CCTCCAGCCA GCCGACACCC ACCATGTCCC CACAGACACC CCAGGACAAG 
CTCACTGCTA ATGAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC 
TCCTTTACAC CTTTTATAGA CCCCAGGTTA CTACAGATCT CTCCATCTAG TGGAACAACA 
GTTACTTCCG TTGTGGGATT TTCCTGTGAT GGGCTGAGAC CAGAAGCCAT AAGGCAAGAT 
CCTACTCGGA AAGGCTCAGT GGTCAATGTG AACCCCACGA ACACTAGGCC ACAGAGTGAT 
ACCCCCGAGA TTCGTAAATA CAAGAAGAGA TTTAACTCAG AGATCCTGTG TGCTGCCTTA 
TGGGGAGTGA ATTTGCTAGT TGGCACAGAG AGTGGCCTGA TGCTCTTGGA CAGAAGTGGC 
CAAGGGAAAG TGTACCCTCT GATCAGCCGG AGACGGTTCC AGCAAATGGA TGTACTTGAG 
GGCCTGAATG TCCTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGCGT CTACTATTTA 
TCCTGGTTAA GAAATAAAAT ACTTCATAAT GATCCGGAAG TTGAGAAGAA GCAGGGGTGG 
ACCACCGTGG GCGACCTGGA AGGGTGCGTG CACTATAAAG TCGTAAAATA TGAAAGAATC 
AAGTTTCTGG TAATCGCTTT GAAGAGTTCT GTGGAAGTCT ATGCATGGGC ACCGAAGCCA 
TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAACTGG TACATAAGCC ATTACTGGTG 
GATCTCACTG TCGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC 
CATGCTGTTG ATGTGGATTC CGGCTCAGTC TATGACATTT ATCTACCAAC ACACGTAAGA 
AAGAACCCAC ACTCTATGAT TCAGTGTACC ATCAAACCCC ATGCAATCAT TATCCTCCCC 
AACACGGATG GAATGGAGCT TCTGGTGTGC TACGAAGATG AAGGGGTTTA TGTGAACACG 
TACGGAAGAA TCACCAAGGA CGTGGTTCTG CAGTGGGGAG AGATGCCAAC ATCTGTAGCA 
TACATCCGGT CGAATCAGAC AATGGGCTGG GGAGAGAAGG CAATAGAGAT ACGCTCTGTG 
GAAACTGGTC ATTTGGACGG TGTGTTTATG CACAAAAGGG CTCAGAGACT AAAGTTCCTG 
TGTGAACGTA ATGACAAGGT GTTCTTTGCT TCCGTCCGCT CTGGCGGCAG CAGCCAGGTC 
TACTTCATGA CCTTAGGCAG GACTTCCCTT CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765231.2
CDS	210..4151
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVEDRFRKTNHSSPEAQAKQTGRGLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008767009.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767009.2

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG 
GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT 
TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG 
GTTCCTGTGA GAACGACATC TCGTTCCCCT GTCCTGTCCC GTCGAGATTC TCCACTGCAG 
GGCAGTGGGC AGCAAAATAG CCAAGCAGGA CAGAGAAATT CCACCAGCAG TATTGAGCCC 
CGGCTTCTAT GGGAGAGAGT GGAAAAGCTG GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA 
GGGTCCAGCA ACTCAGGATC CCAGCCTGGC TCCCATCCTG GGTCTCAGAG TGGCTCTGGT 
GAACGCTTCA GAGTGAGATC ATCATCCAAG TCTGAAGGTT CTCCATCTCA GCGCCTTGAA 
AATGCAGCCA AAAAACCTGA AGATAAGAAA GAAGTATTTC GACCTCTCAA ACCTGCTGGA 
GAAGTGGACT TGACTGCGTT GGCCAAAGAG CTTCGAGCAG TGGAAGATGT GCGGCCACCT 
CACAAAGTGA CAGACTATTC CTCCTCCAGT GAGGAGTCTG GGACCACGGA TGAGGAGGAG 
GAGGATGTGG AACAGGAGGG GGCTGATGAT TCCACCTCGG GGCCAGAGGA CACCAGAGCA 
GCGTCGTCTC TGAATCTGAG CAACGGTGAG ACAGAATCTG TGAAAACAAT GATTGTTCAT 
GATGACGTAG AAAGTGAACC AGCCATGACC CCATCCAAGG AGGGCACCCT AATCGTCCGC 
CAGAGTACAG TTGACCAAAA GCGTGCCAGC CATCATGAGA GCAATGGCTT TGCGGGTCGC 
ATTCACCTCT TGCCAGATCT CTTACAGCAA AGCCATTCCT CCTCCACTTC CTCCACCTCT 
TCCTCCCCAT CCTCCAGCCA GCCGACACCC ACCATGTCCC CACAGACACC CCAGGACAAG 
CTCACTGCTA ATGAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC 
TCCTTTACAC CTTTTATAGA CCCCAGGTTA CTACAGATCT CTCCATCTAG TGGAACAACA 
GTTACTTCCG TTGTGGGATT TTCCTGTGAT GGGCTGAGAC CAGAAGCCAT AAGGCAAGAT 
CCTACTCGGA AAGGCTCAGT GGTCAATGTG AACCCCACGA ACACTAGGCC ACAGAGTGAT 
ACCCCCGAGA TTCGTAAATA CAAGAAGAGA TTTAACTCAG AGATCCTGTG TGCTGCCTTA 
TGGGGAGTGA ATTTGCTAGT TGGCACAGAG AGTGGCCTGA TGCTCTTGGA CAGAAGTGGC 
CAAGGGAAAG TGTACCCTCT GATCAGCCGG AGACGGTTCC AGCAAATGGA TGTACTTGAG 
GGCCTGAATG TCCTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGCGT CTACTATTTA 
TCCTGGTTAA GAAATAAAAT ACTTCATAAT GATCCGGAAG TTGAGAAGAA GCAGGGGTGG 
ACCACCGTGG GCGACCTGGA AGGGTGCGTG CACTATAAAG TCGTAAAATA TGAAAGAATC 
AAGTTTCTGG TAATCGCTTT GAAGAGTTCT GTGGAAGTCT ATGCATGGGC ACCGAAGCCA 
TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAACTGG TACATAAGCC ATTACTGGTG 
GATCTCACTG TCGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC 
CATGCTGTTG ATGTGGATTC CGGCTCAGTC TATGACATTT ATCTACCAAC ACACGTAAGA 
AAGAACCCAC ACTCTATGAT TCAGTGTACC ATCAAACCCC ATGCAATCAT TATCCTCCCC 
AACACGGATG GAATGGAGCT TCTGGTGTGC TACGAAGATG AAGGGGTTTA TGTGAACACG 
TACGGAAGAA TCACCAAGGA CGTGGTTCTG CAGTGGGGAG AGATGCCAAC ATCTGTAGCA 
TACATCCGGT CGAATCAGAC AATGGGCTGG GGAGAGAAGG CAATAGAGAT ACGCTCTGTG 
GAAACTGGTC ATTTGGACGG TGTGTTTATG CACAAAAGGG CTCAGAGACT AAAGTTCCTG 
TGTGAACGTA ATGACAAGGT GTTCTTTGCT TCCGTCCGCT CTGGCGGCAG CAGCCAGGTC 
TACTTCATGA CCTTAGGCAG GACTTCCCTT CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_008767010.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765232.2 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X12, mRNA.

Price & Availability↑

CloneID	ORa62701
Clone ID Related Accession (Same CDS sequence)	XM_008767010.2
Accession Version	XM_008767010.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTACA GTGGTCCCAC 
CTGGCATCTC TCAAGAACAA TGTCTCCCCT GTCTCGAGAT CCCATTCCTT CAGTGACCCT 
TCTCCCAAAT TCGCACACCA CCATCTCCGC TCTCAGGACC CATGTCCACC TTCCCGCAGT 
GAGGGGCTCA GTCAGAGCTC TGACTCTAAG TCGGAGGTAC CTGAGCCCAC CCAAAAGGCT 
TGGTCTAGAT CAGACAGTGA CGAGGTGCCT CCAAGGGTTC CTGTGAGAAC GACATCTCGT 
TCCCCTGTCC TGTCCCGTCG AGATTCTCCA CTGCAGGGCA GTGGGCAGCA AAATAGCCAA 
GCAGGACAGA GAAATTCCAC CAGCAGTATT GAGCCCCGGC TTCTATGGGA GAGAGTGGAA 
AAGCTGGTCC CCAGGCCAGG CAGTGGCAGC TCTTCAGGGT CCAGCAACTC AGGATCCCAG 
CCTGGCTCCC ATCCTGGGTC TCAGAGTGGC TCTGGTGAAC GCTTCAGAGT GAGATCATCA 
TCCAAGTCTG AAGGTTCTCC ATCTCAGCGC CTTGAAAATG CAGCCAAAAA ACCTGAAGAT 
AAGAAAGAAG TATTTCGACC TCTCAAACCT GCTGGAGAAG TGGACTTGAC TGCGTTGGCC 
AAAGAGCTTC GAGCAGTGGA AGATGTGCGG CCACCTCACA AAGTGACAGA CTATTCCTCC 
TCCAGTGAGG AGTCTGGGAC CACGGATGAG GAGGAGGAGG ATGTGGAACA GGAGGGGGCT 
GATGATTCCA CCTCGGGGCC AGAGGACACC AGAGCAGCGT CGTCTCTGAA TCTGAGCAAC 
GGTGAGACAG AATCTGTGAA AACAATGATT GTTCATGATG ACGTAGAAAG TGAACCAGCC 
ATGACCCCAT CCAAGGAGGG CACCCTAATC GTCCGCCAGA GTACAGTTGA CCAAAAGCGT 
GCCAGCCATC ATGAGAGCAA TGGCTTTGCG GGTCGCATTC ACCTCTTGCC AGATCTCTTA 
CAGCAAAGCC ATTCCTCCTC CACTTCCTCC ACCTCTTCCT CCCCATCCTC CAGCCAGCCG 
ACACCCACCA TGTCCCCACA GACACCCCAG GACAAGCTCA CTGCTAATGA GACTCAGTCC 
GCTAGTAGCA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC 
AGGTTACTAC AGATCTCTCC ATCTAGTGGA ACAACAGTTA CTTCCGTTGT GGGATTTTCC 
TGTGATGGGC TGAGACCAGA AGCCATAAGG CAAGATCCTA CTCGGAAAGG CTCAGTGGTC 
AATGTGAACC CCACGAACAC TAGGCCACAG AGTGATACCC CCGAGATTCG TAAATACAAG 
AAGAGATTTA ACTCAGAGAT CCTGTGTGCT GCCTTATGGG GAGTGAATTT GCTAGTTGGC 
ACAGAGAGTG GCCTGATGCT CTTGGACAGA AGTGGCCAAG GGAAAGTGTA CCCTCTGATC 
AGCCGGAGAC GGTTCCAGCA AATGGATGTA CTTGAGGGCC TGAATGTCCT GGTGACAATA 
TCTGGCAAAA AGGATAAGTT ACGCGTCTAC TATTTATCCT GGTTAAGAAA TAAAATACTT 
CATAATGATC CGGAAGTTGA GAAGAAGCAG GGGTGGACCA CCGTGGGCGA CCTGGAAGGG 
TGCGTGCACT ATAAAGTCGT AAAATATGAA AGAATCAAGT TTCTGGTAAT CGCTTTGAAG 
AGTTCTGTGG AAGTCTATGC ATGGGCACCG AAGCCATATC ACAAATTTAT GGCCTTTAAG 
TCATTTGGAG AACTGGTACA TAAGCCATTA CTGGTGGATC TCACTGTCGA GGAAGGCCAG 
AGGTTGAAAG TGATCTATGG ATCCTGTGCT GGATTCCATG CTGTTGATGT GGATTCCGGC 
TCAGTCTATG ACATTTATCT ACCAACACAC GTAAGAAAGA ACCCACACTC TATGATTCAG 
TGTACCATCA AACCCCATGC AATCATTATC CTCCCCAACA CGGATGGAAT GGAGCTTCTG 
GTGTGCTACG AAGATGAAGG GGTTTATGTG AACACGTACG GAAGAATCAC CAAGGACGTG 
GTTCTGCAGT GGGGAGAGAT GCCAACATCT GTAGCATACA TCCGGTCGAA TCAGACAATG 
GGCTGGGGAG AGAAGGCAAT AGAGATACGC TCTGTGGAAA CTGGTCATTT GGACGGTGTG 
TTTATGCACA AAAGGGCTCA GAGACTAAAG TTCCTGTGTG AACGTAATGA CAAGGTGTTC 
TTTGCTTCCG TCCGCTCTGG CGGCAGCAGC CAGGTCTACT TCATGACCTT AGGCAGGACT 
TCCCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765232.2
CDS	210..4127
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008767010.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767010.2

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTACA GTGGTCCCAC 
CTGGCATCTC TCAAGAACAA TGTCTCCCCT GTCTCGAGAT CCCATTCCTT CAGTGACCCT 
TCTCCCAAAT TCGCACACCA CCATCTCCGC TCTCAGGACC CATGTCCACC TTCCCGCAGT 
GAGGGGCTCA GTCAGAGCTC TGACTCTAAG TCGGAGGTAC CTGAGCCCAC CCAAAAGGCT 
TGGTCTAGAT CAGACAGTGA CGAGGTGCCT CCAAGGGTTC CTGTGAGAAC GACATCTCGT 
TCCCCTGTCC TGTCCCGTCG AGATTCTCCA CTGCAGGGCA GTGGGCAGCA AAATAGCCAA 
GCAGGACAGA GAAATTCCAC CAGCAGTATT GAGCCCCGGC TTCTATGGGA GAGAGTGGAA 
AAGCTGGTCC CCAGGCCAGG CAGTGGCAGC TCTTCAGGGT CCAGCAACTC AGGATCCCAG 
CCTGGCTCCC ATCCTGGGTC TCAGAGTGGC TCTGGTGAAC GCTTCAGAGT GAGATCATCA 
TCCAAGTCTG AAGGTTCTCC ATCTCAGCGC CTTGAAAATG CAGCCAAAAA ACCTGAAGAT 
AAGAAAGAAG TATTTCGACC TCTCAAACCT GCTGGAGAAG TGGACTTGAC TGCGTTGGCC 
AAAGAGCTTC GAGCAGTGGA AGATGTGCGG CCACCTCACA AAGTGACAGA CTATTCCTCC 
TCCAGTGAGG AGTCTGGGAC CACGGATGAG GAGGAGGAGG ATGTGGAACA GGAGGGGGCT 
GATGATTCCA CCTCGGGGCC AGAGGACACC AGAGCAGCGT CGTCTCTGAA TCTGAGCAAC 
GGTGAGACAG AATCTGTGAA AACAATGATT GTTCATGATG ACGTAGAAAG TGAACCAGCC 
ATGACCCCAT CCAAGGAGGG CACCCTAATC GTCCGCCAGA GTACAGTTGA CCAAAAGCGT 
GCCAGCCATC ATGAGAGCAA TGGCTTTGCG GGTCGCATTC ACCTCTTGCC AGATCTCTTA 
CAGCAAAGCC ATTCCTCCTC CACTTCCTCC ACCTCTTCCT CCCCATCCTC CAGCCAGCCG 
ACACCCACCA TGTCCCCACA GACACCCCAG GACAAGCTCA CTGCTAATGA GACTCAGTCC 
GCTAGTAGCA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC 
AGGTTACTAC AGATCTCTCC ATCTAGTGGA ACAACAGTTA CTTCCGTTGT GGGATTTTCC 
TGTGATGGGC TGAGACCAGA AGCCATAAGG CAAGATCCTA CTCGGAAAGG CTCAGTGGTC 
AATGTGAACC CCACGAACAC TAGGCCACAG AGTGATACCC CCGAGATTCG TAAATACAAG 
AAGAGATTTA ACTCAGAGAT CCTGTGTGCT GCCTTATGGG GAGTGAATTT GCTAGTTGGC 
ACAGAGAGTG GCCTGATGCT CTTGGACAGA AGTGGCCAAG GGAAAGTGTA CCCTCTGATC 
AGCCGGAGAC GGTTCCAGCA AATGGATGTA CTTGAGGGCC TGAATGTCCT GGTGACAATA 
TCTGGCAAAA AGGATAAGTT ACGCGTCTAC TATTTATCCT GGTTAAGAAA TAAAATACTT 
CATAATGATC CGGAAGTTGA GAAGAAGCAG GGGTGGACCA CCGTGGGCGA CCTGGAAGGG 
TGCGTGCACT ATAAAGTCGT AAAATATGAA AGAATCAAGT TTCTGGTAAT CGCTTTGAAG 
AGTTCTGTGG AAGTCTATGC ATGGGCACCG AAGCCATATC ACAAATTTAT GGCCTTTAAG 
TCATTTGGAG AACTGGTACA TAAGCCATTA CTGGTGGATC TCACTGTCGA GGAAGGCCAG 
AGGTTGAAAG TGATCTATGG ATCCTGTGCT GGATTCCATG CTGTTGATGT GGATTCCGGC 
TCAGTCTATG ACATTTATCT ACCAACACAC GTAAGAAAGA ACCCACACTC TATGATTCAG 
TGTACCATCA AACCCCATGC AATCATTATC CTCCCCAACA CGGATGGAAT GGAGCTTCTG 
GTGTGCTACG AAGATGAAGG GGTTTATGTG AACACGTACG GAAGAATCAC CAAGGACGTG 
GTTCTGCAGT GGGGAGAGAT GCCAACATCT GTAGCATACA TCCGGTCGAA TCAGACAATG 
GGCTGGGGAG AGAAGGCAAT AGAGATACGC TCTGTGGAAA CTGGTCATTT GGACGGTGTG 
TTTATGCACA AAAGGGCTCA GAGACTAAAG TTCCTGTGTG AACGTAATGA CAAGGTGTTC 
TTTGCTTCCG TCCGCTCTGG CGGCAGCAGC CAGGTCTACT TCATGACCTT AGGCAGGACT 
TCCCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_017596341.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451830.1 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X13, mRNA.

Price & Availability↑

CloneID	ORa62702
Clone ID Related Accession (Same CDS sequence)	XM_017596341.1
Accession Version	XM_017596341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT 
AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG 
CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT 
GCCCTGCAGA GACCAGCGGA GCCACAGGTT CCTGTGAGAA CGACATCTCG TTCCCCTGTC 
CTGTCCCGTC GAGATTCTCC ACTGCAGGGC AGTGGGCAGC AAAATAGCCA AGCAGGACAG 
AGAAATTCCA CCAGCAGTAT TGAGCCCCGG CTTCTATGGG AGAGAGTGGA AAAGCTGGTC 
CCCAGGCCAG GCAGTGGCAG CTCTTCAGGG TCCAGCAACT CAGGATCCCA GCCTGGCTCC 
CATCCTGGGT CTCAGAGTGG CTCTGGTGAA CGCTTCAGAG TGAGATCATC ATCCAAGTCT 
GAAGGTTCTC CATCTCAGCG CCTTGAAAAT GCAGCCAAAA AACCTGAAGA TAAGAAAGAA 
GTATTTCGAC CTCTCAAACC TGCTGGAGAA GTGGACTTGA CTGCGTTGGC CAAAGAGCTT 
CGAGCAGTGG AAGATGTGCG GCCACCTCAC AAAGTGACAG ACTATTCCTC CTCCAGTGAG 
GAGTCTGGGA CCACGGATGA GGAGGAGGAG GATGTGGAAC AGGAGGGGGC TGATGATTCC 
ACCTCGGGGC CAGAGGACAC CAGAGCAGCG TCGTCTCTGA ATCTGAGCAA CGGTGAGACA 
GAATCTGTGA AAACAATGAT TGTTCATGAT GACGTAGAAA GTGAACCAGC CATGACCCCA 
TCCAAGGAGG GCACCCTAAT CGTCCGCCAG AGTACAGTTG ACCAAAAGCG TGCCAGCCAT 
CATGAGAGCA ATGGCTTTGC GGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC 
CATTCCTCCT CCACTTCCTC CACCTCTTCC TCCCCATCCT CCAGCCAGCC GACACCCACC 
ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTGCTAATG AGACTCAGTC CGCTAGTAGC 
ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGGTTACTA 
CAGATCTCTC CATCTAGTGG AACAACAGTT ACTTCCGTTG TGGGATTTTC CTGTGATGGG 
CTGAGACCAG AAGCCATAAG GCAAGATCCT ACTCGGAAAG GCTCAGTGGT CAATGTGAAC 
CCCACGAACA CTAGGCCACA GAGTGATACC CCCGAGATTC GTAAATACAA GAAGAGATTT 
AACTCAGAGA TCCTGTGTGC TGCCTTATGG GGAGTGAATT TGCTAGTTGG CACAGAGAGT 
GGCCTGATGC TCTTGGACAG AAGTGGCCAA GGGAAAGTGT ACCCTCTGAT CAGCCGGAGA 
CGGTTCCAGC AAATGGATGT ACTTGAGGGC CTGAATGTCC TGGTGACAAT ATCTGGCAAA 
AAGGATAAGT TACGCGTCTA CTATTTATCC TGGTTAAGAA ATAAAATACT TCATAATGAT 
CCGGAAGTTG AGAAGAAGCA GGGGTGGACC ACCGTGGGCG ACCTGGAAGG GTGCGTGCAC 
TATAAAGTCG TAAAATATGA AAGAATCAAG TTTCTGGTAA TCGCTTTGAA GAGTTCTGTG 
GAAGTCTATG CATGGGCACC GAAGCCATAT CACAAATTTA TGGCCTTTAA GTCATTTGGA 
GAACTGGTAC ATAAGCCATT ACTGGTGGAT CTCACTGTCG AGGAAGGCCA GAGGTTGAAA 
GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGATTCCGG CTCAGTCTAT 
GACATTTATC TACCAACACA CGTAAGAAAG AACCCACACT CTATGATTCA GTGTACCATC 
AAACCCCATG CAATCATTAT CCTCCCCAAC ACGGATGGAA TGGAGCTTCT GGTGTGCTAC 
GAAGATGAAG GGGTTTATGT GAACACGTAC GGAAGAATCA CCAAGGACGT GGTTCTGCAG 
TGGGGAGAGA TGCCAACATC TGTAGCATAC ATCCGGTCGA ATCAGACAAT GGGCTGGGGA 
GAGAAGGCAA TAGAGATACG CTCTGTGGAA ACTGGTCATT TGGACGGTGT GTTTATGCAC 
AAAAGGGCTC AGAGACTAAA GTTCCTGTGT GAACGTAATG ACAAGGTGTT CTTTGCTTCC 
GTCCGCTCTG GCGGCAGCAG CCAGGTCTAC TTCATGACCT TAGGCAGGAC TTCCCTTCTG 
AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451830.1
CDS	210..4058
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVEDRFRKTNHSSPEAQAKQTGRGLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017596341.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596341.1

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT 
AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG 
CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT 
GCCCTGCAGA GACCAGCGGA GCCACAGGTT CCTGTGAGAA CGACATCTCG TTCCCCTGTC 
CTGTCCCGTC GAGATTCTCC ACTGCAGGGC AGTGGGCAGC AAAATAGCCA AGCAGGACAG 
AGAAATTCCA CCAGCAGTAT TGAGCCCCGG CTTCTATGGG AGAGAGTGGA AAAGCTGGTC 
CCCAGGCCAG GCAGTGGCAG CTCTTCAGGG TCCAGCAACT CAGGATCCCA GCCTGGCTCC 
CATCCTGGGT CTCAGAGTGG CTCTGGTGAA CGCTTCAGAG TGAGATCATC ATCCAAGTCT 
GAAGGTTCTC CATCTCAGCG CCTTGAAAAT GCAGCCAAAA AACCTGAAGA TAAGAAAGAA 
GTATTTCGAC CTCTCAAACC TGCTGGAGAA GTGGACTTGA CTGCGTTGGC CAAAGAGCTT 
CGAGCAGTGG AAGATGTGCG GCCACCTCAC AAAGTGACAG ACTATTCCTC CTCCAGTGAG 
GAGTCTGGGA CCACGGATGA GGAGGAGGAG GATGTGGAAC AGGAGGGGGC TGATGATTCC 
ACCTCGGGGC CAGAGGACAC CAGAGCAGCG TCGTCTCTGA ATCTGAGCAA CGGTGAGACA 
GAATCTGTGA AAACAATGAT TGTTCATGAT GACGTAGAAA GTGAACCAGC CATGACCCCA 
TCCAAGGAGG GCACCCTAAT CGTCCGCCAG AGTACAGTTG ACCAAAAGCG TGCCAGCCAT 
CATGAGAGCA ATGGCTTTGC GGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC 
CATTCCTCCT CCACTTCCTC CACCTCTTCC TCCCCATCCT CCAGCCAGCC GACACCCACC 
ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTGCTAATG AGACTCAGTC CGCTAGTAGC 
ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGGTTACTA 
CAGATCTCTC CATCTAGTGG AACAACAGTT ACTTCCGTTG TGGGATTTTC CTGTGATGGG 
CTGAGACCAG AAGCCATAAG GCAAGATCCT ACTCGGAAAG GCTCAGTGGT CAATGTGAAC 
CCCACGAACA CTAGGCCACA GAGTGATACC CCCGAGATTC GTAAATACAA GAAGAGATTT 
AACTCAGAGA TCCTGTGTGC TGCCTTATGG GGAGTGAATT TGCTAGTTGG CACAGAGAGT 
GGCCTGATGC TCTTGGACAG AAGTGGCCAA GGGAAAGTGT ACCCTCTGAT CAGCCGGAGA 
CGGTTCCAGC AAATGGATGT ACTTGAGGGC CTGAATGTCC TGGTGACAAT ATCTGGCAAA 
AAGGATAAGT TACGCGTCTA CTATTTATCC TGGTTAAGAA ATAAAATACT TCATAATGAT 
CCGGAAGTTG AGAAGAAGCA GGGGTGGACC ACCGTGGGCG ACCTGGAAGG GTGCGTGCAC 
TATAAAGTCG TAAAATATGA AAGAATCAAG TTTCTGGTAA TCGCTTTGAA GAGTTCTGTG 
GAAGTCTATG CATGGGCACC GAAGCCATAT CACAAATTTA TGGCCTTTAA GTCATTTGGA 
GAACTGGTAC ATAAGCCATT ACTGGTGGAT CTCACTGTCG AGGAAGGCCA GAGGTTGAAA 
GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGATTCCGG CTCAGTCTAT 
GACATTTATC TACCAACACA CGTAAGAAAG AACCCACACT CTATGATTCA GTGTACCATC 
AAACCCCATG CAATCATTAT CCTCCCCAAC ACGGATGGAA TGGAGCTTCT GGTGTGCTAC 
GAAGATGAAG GGGTTTATGT GAACACGTAC GGAAGAATCA CCAAGGACGT GGTTCTGCAG 
TGGGGAGAGA TGCCAACATC TGTAGCATAC ATCCGGTCGA ATCAGACAAT GGGCTGGGGA 
GAGAAGGCAA TAGAGATACG CTCTGTGGAA ACTGGTCATT TGGACGGTGT GTTTATGCAC 
AAAAGGGCTC AGAGACTAAA GTTCCTGTGT GAACGTAATG ACAAGGTGTT CTTTGCTTCC 
GTCCGCTCTG GCGGCAGCAG CCAGGTCTAC TTCATGACCT TAGGCAGGAC TTCCCTTCTG 
AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_008767011.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765233.2 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X14, mRNA.

Price & Availability↑

CloneID	ORa62703
Clone ID Related Accession (Same CDS sequence)	XM_008767011.2
Accession Version	XM_008767011.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT 
AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG 
CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT 
GCCCTGCAGA GACCAGCGGA GCCACAGGTT CCTGTGAGAA CGACATCTCG TTCCCCTGTC 
CTGTCCCGTC GAGATTCTCC ACTGCAGGGC AGTGGGCAGC AAAATAGCCA AGCAGGACAG 
AGAAATTCCA CCAGCAGTAT TGAGCCCCGG CTTCTATGGG AGAGAGTGGA AAAGCTGGTC 
CCCAGGCCAG GCAGTGGCAG CTCTTCAGGG TCCAGCAACT CAGGATCCCA GCCTGGCTCC 
CATCCTGGGT CTCAGAGTGG CTCTGGTGAA CGCTTCAGAG TGAGATCATC ATCCAAGTCT 
GAAGGTTCTC CATCTCAGCG CCTTGAAAAT GCAGCCAAAA AACCTGAAGA TAAGAAAGAA 
GTATTTCGAC CTCTCAAACC TGCTGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG 
GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG 
ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG 
CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG 
AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG 
GGCACCCTAA TCGTCCGCCA GAGTACAGTT GACCAAAAGC GTGCCAGCCA TCATGAGAGC 
AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
TCCACTTCCT CCACCTCTTC CTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
CAGACACCCC AGGACAAGCT CACTGCTAAT GAGACTCAGT CCGCTAGTAG CACACTCCAG 
AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGGTTACT ACAGATCTCT 
CCATCTAGTG GAACAACAGT TACTTCCGTT GTGGGATTTT CCTGTGATGG GCTGAGACCA 
GAAGCCATAA GGCAAGATCC TACTCGGAAA GGCTCAGTGG TCAATGTGAA CCCCACGAAC 
ACTAGGCCAC AGAGTGATAC CCCCGAGATT CGTAAATACA AGAAGAGATT TAACTCAGAG 
ATCCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTTG GCACAGAGAG TGGCCTGATG 
CTCTTGGACA GAAGTGGCCA AGGGAAAGTG TACCCTCTGA TCAGCCGGAG ACGGTTCCAG 
CAAATGGATG TACTTGAGGG CCTGAATGTC CTGGTGACAA TATCTGGCAA AAAGGATAAG 
TTACGCGTCT ACTATTTATC CTGGTTAAGA AATAAAATAC TTCATAATGA TCCGGAAGTT 
GAGAAGAAGC AGGGGTGGAC CACCGTGGGC GACCTGGAAG GGTGCGTGCA CTATAAAGTC 
GTAAAATATG AAAGAATCAA GTTTCTGGTA ATCGCTTTGA AGAGTTCTGT GGAAGTCTAT 
GCATGGGCAC CGAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAACTGGTA 
CATAAGCCAT TACTGGTGGA TCTCACTGTC GAGGAAGGCC AGAGGTTGAA AGTGATCTAT 
GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCCG GCTCAGTCTA TGACATTTAT 
CTACCAACAC ACATTCAGTG TACCATCAAA CCCCATGCAA TCATTATCCT CCCCAACACG 
GATGGAATGG AGCTTCTGGT GTGCTACGAA GATGAAGGGG TTTATGTGAA CACGTACGGA 
AGAATCACCA AGGACGTGGT TCTGCAGTGG GGAGAGATGC CAACATCTGT AGCATACATC 
CGGTCGAATC AGACAATGGG CTGGGGAGAG AAGGCAATAG AGATACGCTC TGTGGAAACT 
GGTCATTTGG ACGGTGTGTT TATGCACAAA AGGGCTCAGA GACTAAAGTT CCTGTGTGAA 
CGTAATGACA AGGTGTTCTT TGCTTCCGTC CGCTCTGGCG GCAGCAGCCA GGTCTACTTC 
ATGACCTTAG GCAGGACTTC CCTTCTGAGC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765233.2
CDS	208..4023
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVEDRFRKTNHSSPEAQAKQTGRGLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008767011.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767011.2

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT 
AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG 
CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT 
GCCCTGCAGA GACCAGCGGA GCCACAGGTT CCTGTGAGAA CGACATCTCG TTCCCCTGTC 
CTGTCCCGTC GAGATTCTCC ACTGCAGGGC AGTGGGCAGC AAAATAGCCA AGCAGGACAG 
AGAAATTCCA CCAGCAGTAT TGAGCCCCGG CTTCTATGGG AGAGAGTGGA AAAGCTGGTC 
CCCAGGCCAG GCAGTGGCAG CTCTTCAGGG TCCAGCAACT CAGGATCCCA GCCTGGCTCC 
CATCCTGGGT CTCAGAGTGG CTCTGGTGAA CGCTTCAGAG TGAGATCATC ATCCAAGTCT 
GAAGGTTCTC CATCTCAGCG CCTTGAAAAT GCAGCCAAAA AACCTGAAGA TAAGAAAGAA 
GTATTTCGAC CTCTCAAACC TGCTGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG 
GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG 
ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG 
CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG 
AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG 
GGCACCCTAA TCGTCCGCCA GAGTACAGTT GACCAAAAGC GTGCCAGCCA TCATGAGAGC 
AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
TCCACTTCCT CCACCTCTTC CTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
CAGACACCCC AGGACAAGCT CACTGCTAAT GAGACTCAGT CCGCTAGTAG CACACTCCAG 
AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGGTTACT ACAGATCTCT 
CCATCTAGTG GAACAACAGT TACTTCCGTT GTGGGATTTT CCTGTGATGG GCTGAGACCA 
GAAGCCATAA GGCAAGATCC TACTCGGAAA GGCTCAGTGG TCAATGTGAA CCCCACGAAC 
ACTAGGCCAC AGAGTGATAC CCCCGAGATT CGTAAATACA AGAAGAGATT TAACTCAGAG 
ATCCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTTG GCACAGAGAG TGGCCTGATG 
CTCTTGGACA GAAGTGGCCA AGGGAAAGTG TACCCTCTGA TCAGCCGGAG ACGGTTCCAG 
CAAATGGATG TACTTGAGGG CCTGAATGTC CTGGTGACAA TATCTGGCAA AAAGGATAAG 
TTACGCGTCT ACTATTTATC CTGGTTAAGA AATAAAATAC TTCATAATGA TCCGGAAGTT 
GAGAAGAAGC AGGGGTGGAC CACCGTGGGC GACCTGGAAG GGTGCGTGCA CTATAAAGTC 
GTAAAATATG AAAGAATCAA GTTTCTGGTA ATCGCTTTGA AGAGTTCTGT GGAAGTCTAT 
GCATGGGCAC CGAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAACTGGTA 
CATAAGCCAT TACTGGTGGA TCTCACTGTC GAGGAAGGCC AGAGGTTGAA AGTGATCTAT 
GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCCG GCTCAGTCTA TGACATTTAT 
CTACCAACAC ACATTCAGTG TACCATCAAA CCCCATGCAA TCATTATCCT CCCCAACACG 
GATGGAATGG AGCTTCTGGT GTGCTACGAA GATGAAGGGG TTTATGTGAA CACGTACGGA 
AGAATCACCA AGGACGTGGT TCTGCAGTGG GGAGAGATGC CAACATCTGT AGCATACATC 
CGGTCGAATC AGACAATGGG CTGGGGAGAG AAGGCAATAG AGATACGCTC TGTGGAAACT 
GGTCATTTGG ACGGTGTGTT TATGCACAAA AGGGCTCAGA GACTAAAGTT CCTGTGTGAA 
CGTAATGACA AGGTGTTCTT TGCTTCCGTC CGCTCTGGCG GCAGCAGCCA GGTCTACTTC 
ATGACCTTAG GCAGGACTTC CCTTCTGAGC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_008767012.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765234.2 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X15, mRNA.

Price & Availability↑

CloneID	ORa62704
Clone ID Related Accession (Same CDS sequence)	XM_008767012.2
Accession Version	XM_008767012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT TCCTGTGAGA ACGACATCTC GTTCCCCTGT CCTGTCCCGT 
CGAGATTCTC CACTGCAGGG CAGTGGGCAG CAAAATAGCC AAGCAGGACA GAGAAATTCC 
ACCAGCAGTA TTGAGCCCCG GCTTCTATGG GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA 
GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC TCAGGATCCC AGCCTGGCTC CCATCCTGGG 
TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA GTGAGATCAT CATCCAAGTC TGAAGGTTCT 
CCATCTCAGC GCCTTGAAAA TGCAGCCAAA AAACCTGAAG ATAAGAAAGA AGTATTTCGA 
CCTCTCAAAC CTGCTGGAGA AGTGGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG 
GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG 
ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG 
CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG 
AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG 
GGCACCCTAA TCGTCCGCCA GAGTACAGTT GACCAAAAGC GTGCCAGCCA TCATGAGAGC 
AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
TCCACTTCCT CCACCTCTTC CTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
CAGACACCCC AGGACAAGCT CACTGCTAAT GAGACTCAGT CCGCTAGTAG CACACTCCAG 
AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGGTTACT ACAGATCTCT 
CCATCTAGTG GAACAACAGT TACTTCCGTT GTGGGATTTT CCTGTGATGG GCTGAGACCA 
GAAGCCATAA GGCAAGATCC TACTCGGAAA GGCTCAGTGG TCAATGTGAA CCCCACGAAC 
ACTAGGCCAC AGAGTGATAC CCCCGAGATT CGTAAATACA AGAAGAGATT TAACTCAGAG 
ATCCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTTG GCACAGAGAG TGGCCTGATG 
CTCTTGGACA GAAGTGGCCA AGGGAAAGTG TACCCTCTGA TCAGCCGGAG ACGGTTCCAG 
CAAATGGATG TACTTGAGGG CCTGAATGTC CTGGTGACAA TATCTGGCAA AAAGGATAAG 
TTACGCGTCT ACTATTTATC CTGGTTAAGA AATAAAATAC TTCATAATGA TCCGGAAGTT 
GAGAAGAAGC AGGGGTGGAC CACCGTGGGC GACCTGGAAG GGTGCGTGCA CTATAAAGTC 
GTAAAATATG AAAGAATCAA GTTTCTGGTA ATCGCTTTGA AGAGTTCTGT GGAAGTCTAT 
GCATGGGCAC CGAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAACTGGTA 
CATAAGCCAT TACTGGTGGA TCTCACTGTC GAGGAAGGCC AGAGGTTGAA AGTGATCTAT 
GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCCG GCTCAGTCTA TGACATTTAT 
CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATTC AGTGTACCAT CAAACCCCAT 
GCAATCATTA TCCTCCCCAA CACGGATGGA ATGGAGCTTC TGGTGTGCTA CGAAGATGAA 
GGGGTTTATG TGAACACGTA CGGAAGAATC ACCAAGGACG TGGTTCTGCA GTGGGGAGAG 
ATGCCAACAT CTGTAGCATA CATCCGGTCG AATCAGACAA TGGGCTGGGG AGAGAAGGCA 
ATAGAGATAC GCTCTGTGGA AACTGGTCAT TTGGACGGTG TGTTTATGCA CAAAAGGGCT 
CAGAGACTAA AGTTCCTGTG TGAACGTAAT GACAAGGTGT TCTTTGCTTC CGTCCGCTCT 
GGCGGCAGCA GCCAGGTCTA CTTCATGACC TTAGGCAGGA CTTCCCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765234.2
CDS	209..3988
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008767012.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767012.2

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT TCCTGTGAGA ACGACATCTC GTTCCCCTGT CCTGTCCCGT 
CGAGATTCTC CACTGCAGGG CAGTGGGCAG CAAAATAGCC AAGCAGGACA GAGAAATTCC 
ACCAGCAGTA TTGAGCCCCG GCTTCTATGG GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA 
GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC TCAGGATCCC AGCCTGGCTC CCATCCTGGG 
TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA GTGAGATCAT CATCCAAGTC TGAAGGTTCT 
CCATCTCAGC GCCTTGAAAA TGCAGCCAAA AAACCTGAAG ATAAGAAAGA AGTATTTCGA 
CCTCTCAAAC CTGCTGGAGA AGTGGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG 
GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG 
ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG 
CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG 
AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG 
GGCACCCTAA TCGTCCGCCA GAGTACAGTT GACCAAAAGC GTGCCAGCCA TCATGAGAGC 
AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
TCCACTTCCT CCACCTCTTC CTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
CAGACACCCC AGGACAAGCT CACTGCTAAT GAGACTCAGT CCGCTAGTAG CACACTCCAG 
AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGGTTACT ACAGATCTCT 
CCATCTAGTG GAACAACAGT TACTTCCGTT GTGGGATTTT CCTGTGATGG GCTGAGACCA 
GAAGCCATAA GGCAAGATCC TACTCGGAAA GGCTCAGTGG TCAATGTGAA CCCCACGAAC 
ACTAGGCCAC AGAGTGATAC CCCCGAGATT CGTAAATACA AGAAGAGATT TAACTCAGAG 
ATCCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTTG GCACAGAGAG TGGCCTGATG 
CTCTTGGACA GAAGTGGCCA AGGGAAAGTG TACCCTCTGA TCAGCCGGAG ACGGTTCCAG 
CAAATGGATG TACTTGAGGG CCTGAATGTC CTGGTGACAA TATCTGGCAA AAAGGATAAG 
TTACGCGTCT ACTATTTATC CTGGTTAAGA AATAAAATAC TTCATAATGA TCCGGAAGTT 
GAGAAGAAGC AGGGGTGGAC CACCGTGGGC GACCTGGAAG GGTGCGTGCA CTATAAAGTC 
GTAAAATATG AAAGAATCAA GTTTCTGGTA ATCGCTTTGA AGAGTTCTGT GGAAGTCTAT 
GCATGGGCAC CGAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAACTGGTA 
CATAAGCCAT TACTGGTGGA TCTCACTGTC GAGGAAGGCC AGAGGTTGAA AGTGATCTAT 
GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCCG GCTCAGTCTA TGACATTTAT 
CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATTC AGTGTACCAT CAAACCCCAT 
GCAATCATTA TCCTCCCCAA CACGGATGGA ATGGAGCTTC TGGTGTGCTA CGAAGATGAA 
GGGGTTTATG TGAACACGTA CGGAAGAATC ACCAAGGACG TGGTTCTGCA GTGGGGAGAG 
ATGCCAACAT CTGTAGCATA CATCCGGTCG AATCAGACAA TGGGCTGGGG AGAGAAGGCA 
ATAGAGATAC GCTCTGTGGA AACTGGTCAT TTGGACGGTG TGTTTATGCA CAAAAGGGCT 
CAGAGACTAA AGTTCCTGTG TGAACGTAAT GACAAGGTGT TCTTTGCTTC CGTCCGCTCT 
GGCGGCAGCA GCCAGGTCTA CTTCATGACC TTAGGCAGGA CTTCCCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_017596342.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451831.1 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X16, mRNA.

Price & Availability↑

CloneID	ORa62705
Clone ID Related Accession (Same CDS sequence)	XM_017596342.1
Accession Version	XM_017596342.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT TCCTGTGAGA ACGACATCTC GTTCCCCTGT CCTGTCCCGT 
CGAGATTCTC CACTGCAGGG CAGTGGGCAG CAAAATAGCC AAGCAGGACA GAGAAATTCC 
ACCAGCAGTA TTGAGCCCCG GCTTCTATGG GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA 
GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC TCAGGATCCC AGCCTGGCTC CCATCCTGGG 
TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA GTGAGATCAT CATCCAAGTC TGAAGGTTCT 
CCATCTCAGC GCCTTGAAAA TGCAGCCAAA AAACCTGAAG ATAAGAAAGA AGTATTTCGA 
CCTCTCAAAC CTGCTGACTT GACTGCGTTG GCCAAAGAGC TTCGAGCAGT GGAAGATGTG 
CGGCCACCTC ACAAAGTGAC AGACTATTCC TCCTCCAGTG AGGAGTCTGG GACCACGGAT 
GAGGAGGAGG AGGATGTGGA ACAGGAGGGG GCTGATGATT CCACCTCGGG GCCAGAGGAC 
ACCAGAGCAG CGTCGTCTCT GAATCTGAGC AACGGTGAGA CAGAATCTGT GAAAACAATG 
ATTGTTCATG ATGACGTAGA AAGTGAACCA GCCATGACCC CATCCAAGGA GGGCACCCTA 
ATCGTCCGCC AGAGTACAGT TGACCAAAAG CGTGCCAGCC ATCATGAGAG CAATGGCTTT 
GCGGGTCGCA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACTTCC 
TCCACCTCTT CCTCCCCATC CTCCAGCCAG CCGACACCCA CCATGTCCCC ACAGACACCC 
CAGGACAAGC TCACTGCTAA TGAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA 
TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGGTTAC TACAGATCTC TCCATCTAGT 
GGAACAACAG TTACTTCCGT TGTGGGATTT TCCTGTGATG GGCTGAGACC AGAAGCCATA 
AGGCAAGATC CTACTCGGAA AGGCTCAGTG GTCAATGTGA ACCCCACGAA CACTAGGCCA 
CAGAGTGATA CCCCCGAGAT TCGTAAATAC AAGAAGAGAT TTAACTCAGA GATCCTGTGT 
GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT GGCACAGAGA GTGGCCTGAT GCTCTTGGAC 
AGAAGTGGCC AAGGGAAAGT GTACCCTCTG ATCAGCCGGA GACGGTTCCA GCAAATGGAT 
GTACTTGAGG GCCTGAATGT CCTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGCGTC 
TACTATTTAT CCTGGTTAAG AAATAAAATA CTTCATAATG ATCCGGAAGT TGAGAAGAAG 
CAGGGGTGGA CCACCGTGGG CGACCTGGAA GGGTGCGTGC ACTATAAAGT CGTAAAATAT 
GAAAGAATCA AGTTTCTGGT AATCGCTTTG AAGAGTTCTG TGGAAGTCTA TGCATGGGCA 
CCGAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAACTGGT ACATAAGCCA 
TTACTGGTGG ATCTCACTGT CGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT 
GCTGGATTCC ATGCTGTTGA TGTGGATTCC GGCTCAGTCT ATGACATTTA TCTACCAACA 
CACATTCAGT GTACCATCAA ACCCCATGCA ATCATTATCC TCCCCAACAC GGATGGAATG 
GAGCTTCTGG TGTGCTACGA AGATGAAGGG GTTTATGTGA ACACGTACGG AAGAATCACC 
AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCAACATCTG TAGCATACAT CCGGTCGAAT 
CAGACAATGG GCTGGGGAGA GAAGGCAATA GAGATACGCT CTGTGGAAAC TGGTCATTTG 
GACGGTGTGT TTATGCACAA AAGGGCTCAG AGACTAAAGT TCCTGTGTGA ACGTAATGAC 
AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC GGCAGCAGCC AGGTCTACTT CATGACCTTA 
GGCAGGACTT CCCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451831.1
CDS	207..3953
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017596342.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596342.1

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT TCCTGTGAGA ACGACATCTC GTTCCCCTGT CCTGTCCCGT 
CGAGATTCTC CACTGCAGGG CAGTGGGCAG CAAAATAGCC AAGCAGGACA GAGAAATTCC 
ACCAGCAGTA TTGAGCCCCG GCTTCTATGG GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA 
GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC TCAGGATCCC AGCCTGGCTC CCATCCTGGG 
TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA GTGAGATCAT CATCCAAGTC TGAAGGTTCT 
CCATCTCAGC GCCTTGAAAA TGCAGCCAAA AAACCTGAAG ATAAGAAAGA AGTATTTCGA 
CCTCTCAAAC CTGCTGACTT GACTGCGTTG GCCAAAGAGC TTCGAGCAGT GGAAGATGTG 
CGGCCACCTC ACAAAGTGAC AGACTATTCC TCCTCCAGTG AGGAGTCTGG GACCACGGAT 
GAGGAGGAGG AGGATGTGGA ACAGGAGGGG GCTGATGATT CCACCTCGGG GCCAGAGGAC 
ACCAGAGCAG CGTCGTCTCT GAATCTGAGC AACGGTGAGA CAGAATCTGT GAAAACAATG 
ATTGTTCATG ATGACGTAGA AAGTGAACCA GCCATGACCC CATCCAAGGA GGGCACCCTA 
ATCGTCCGCC AGAGTACAGT TGACCAAAAG CGTGCCAGCC ATCATGAGAG CAATGGCTTT 
GCGGGTCGCA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACTTCC 
TCCACCTCTT CCTCCCCATC CTCCAGCCAG CCGACACCCA CCATGTCCCC ACAGACACCC 
CAGGACAAGC TCACTGCTAA TGAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA 
TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGGTTAC TACAGATCTC TCCATCTAGT 
GGAACAACAG TTACTTCCGT TGTGGGATTT TCCTGTGATG GGCTGAGACC AGAAGCCATA 
AGGCAAGATC CTACTCGGAA AGGCTCAGTG GTCAATGTGA ACCCCACGAA CACTAGGCCA 
CAGAGTGATA CCCCCGAGAT TCGTAAATAC AAGAAGAGAT TTAACTCAGA GATCCTGTGT 
GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT GGCACAGAGA GTGGCCTGAT GCTCTTGGAC 
AGAAGTGGCC AAGGGAAAGT GTACCCTCTG ATCAGCCGGA GACGGTTCCA GCAAATGGAT 
GTACTTGAGG GCCTGAATGT CCTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGCGTC 
TACTATTTAT CCTGGTTAAG AAATAAAATA CTTCATAATG ATCCGGAAGT TGAGAAGAAG 
CAGGGGTGGA CCACCGTGGG CGACCTGGAA GGGTGCGTGC ACTATAAAGT CGTAAAATAT 
GAAAGAATCA AGTTTCTGGT AATCGCTTTG AAGAGTTCTG TGGAAGTCTA TGCATGGGCA 
CCGAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAACTGGT ACATAAGCCA 
TTACTGGTGG ATCTCACTGT CGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT 
GCTGGATTCC ATGCTGTTGA TGTGGATTCC GGCTCAGTCT ATGACATTTA TCTACCAACA 
CACATTCAGT GTACCATCAA ACCCCATGCA ATCATTATCC TCCCCAACAC GGATGGAATG 
GAGCTTCTGG TGTGCTACGA AGATGAAGGG GTTTATGTGA ACACGTACGG AAGAATCACC 
AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCAACATCTG TAGCATACAT CCGGTCGAAT 
CAGACAATGG GCTGGGGAGA GAAGGCAATA GAGATACGCT CTGTGGAAAC TGGTCATTTG 
GACGGTGTGT TTATGCACAA AAGGGCTCAG AGACTAAAGT TCCTGTGTGA ACGTAATGAC 
AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC GGCAGCAGCC AGGTCTACTT CATGACCTTA 
GGCAGGACTT CCCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_017596343.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451832.1 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X17, mRNA.

Price & Availability↑

CloneID	ORa62706
Clone ID Related Accession (Same CDS sequence)	XM_017596343.1
Accession Version	XM_017596343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG 
GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT 
TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG 
GTTCCTGTGA GAACGACATC TCGTTCCCCT GTCCTGTCCC GTCGAGATTC TCCACTGCAG 
GGCAGTGGGC AGCAAAATAG CCAAGCAGGA CAGAGAAATT CCACCAGTAT TGAGCCCCGG 
CTTCTATGGG AGAGAGTGGA AAAGCTGGTC CCCAGGCCAG GCAGTGGCAG CTCTTCAGGG 
TCCAGCAACT CAGGATCCCA GCCTGGCTCC CATCCTGGGT CTCAGAGTGG CTCTGGTGAA 
CGCTTCAGAG TGAGATCATC ATCCAAGTCT GAAGGTTCTC CATCTCAGCG CCTTGAAAAT 
GCAGCCAAAA AACCTGAAGA TAAGAAAGAA GTATTTCGAC CTCTCAAACC TGCTGACTTG 
ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTGC GGCCACCTCA CAAAGTGACA 
GACTATTCCT CCTCCAGTGA GGAGTCTGGG ACCACGGATG AGGAGGAGGA GGATGTGGAA 
CAGGAGGGGG CTGATGATTC CACCTCGGGG CCAGAGGACA CCAGAGCAGC GTCGTCTCTG 
AATCTGAGCA ACGGTGAGAC AGAATCTGTG AAAACAATGA TTGTTCATGA TGACGTAGAA 
AGTGAACCAG CCATGACCCC ATCCAAGGAG GGCACCCTAA TCGTCCGCCA GACTCAGTCC 
GCTAGTAGCA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC 
AGGTTACTAC AGATCTCTCC ATCTAGTGGA ACAACAGTTA CTTCCGTTGT GGGATTTTCC 
TGTGATGGGC TGAGACCAGA AGCCATAAGG CAAGATCCTA CTCGGAAAGG CTCAGTGGTC 
AATGTGAACC CCACGAACAC TAGGCCACAG AGTGATACCC CCGAGATTCG TAAATACAAG 
AAGAGATTTA ACTCAGAGAT CCTGTGTGCT GCCTTATGGG GAGTGAATTT GCTAGTTGGC 
ACAGAGAGTG GCCTGATGCT CTTGGACAGA AGTGGCCAAG GGAAAGTGTA CCCTCTGATC 
AGCCGGAGAC GGTTCCAGCA AATGGATGTA CTTGAGGGCC TGAATGTCCT GGTGACAATA 
TCTGGCAAAA AGGATAAGTT ACGCGTCTAC TATTTATCCT GGTTAAGAAA TAAAATACTT 
CATAATGATC CGGAAGTTGA GAAGAAGCAG GGGTGGACCA CCGTGGGCGA CCTGGAAGGG 
TGCGTGCACT ATAAAGTCGT AAAATATGAA AGAATCAAGT TTCTGGTAAT CGCTTTGAAG 
AGTTCTGTGG AAGTCTATGC ATGGGCACCG AAGCCATATC ACAAATTTAT GGCCTTTAAG 
TCATTTGGAG AACTGGTACA TAAGCCATTA CTGGTGGATC TCACTGTCGA GGAAGGCCAG 
AGGTTGAAAG TGATCTATGG ATCCTGTGCT GGATTCCATG CTGTTGATGT GGATTCCGGC 
TCAGTCTATG ACATTTATCT ACCAACACAC ATTCAGTGTA CCATCAAACC CCATGCAATC 
ATTATCCTCC CCAACACGGA TGGAATGGAG CTTCTGGTGT GCTACGAAGA TGAAGGGGTT 
TATGTGAACA CGTACGGAAG AATCACCAAG GACGTGGTTC TGCAGTGGGG AGAGATGCCA 
ACATCTGTAG CATACATCCG GTCGAATCAG ACAATGGGCT GGGGAGAGAA GGCAATAGAG 
ATACGCTCTG TGGAAACTGG TCATTTGGAC GGTGTGTTTA TGCACAAAAG GGCTCAGAGA 
CTAAAGTTCC TGTGTGAACG TAATGACAAG GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC 
AGCAGCCAGG TCTACTTCAT GACCTTAGGC AGGACTTCCC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451832.1
CDS	209..3922
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVEDRFRKTNHSSPEAQAKQTGRGLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017596343.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596343.1

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG 
CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT 
GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG 
GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT 
TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG 
GTTCCTGTGA GAACGACATC TCGTTCCCCT GTCCTGTCCC GTCGAGATTC TCCACTGCAG 
GGCAGTGGGC AGCAAAATAG CCAAGCAGGA CAGAGAAATT CCACCAGTAT TGAGCCCCGG 
CTTCTATGGG AGAGAGTGGA AAAGCTGGTC CCCAGGCCAG GCAGTGGCAG CTCTTCAGGG 
TCCAGCAACT CAGGATCCCA GCCTGGCTCC CATCCTGGGT CTCAGAGTGG CTCTGGTGAA 
CGCTTCAGAG TGAGATCATC ATCCAAGTCT GAAGGTTCTC CATCTCAGCG CCTTGAAAAT 
GCAGCCAAAA AACCTGAAGA TAAGAAAGAA GTATTTCGAC CTCTCAAACC TGCTGACTTG 
ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTGC GGCCACCTCA CAAAGTGACA 
GACTATTCCT CCTCCAGTGA GGAGTCTGGG ACCACGGATG AGGAGGAGGA GGATGTGGAA 
CAGGAGGGGG CTGATGATTC CACCTCGGGG CCAGAGGACA CCAGAGCAGC GTCGTCTCTG 
AATCTGAGCA ACGGTGAGAC AGAATCTGTG AAAACAATGA TTGTTCATGA TGACGTAGAA 
AGTGAACCAG CCATGACCCC ATCCAAGGAG GGCACCCTAA TCGTCCGCCA GACTCAGTCC 
GCTAGTAGCA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC 
AGGTTACTAC AGATCTCTCC ATCTAGTGGA ACAACAGTTA CTTCCGTTGT GGGATTTTCC 
TGTGATGGGC TGAGACCAGA AGCCATAAGG CAAGATCCTA CTCGGAAAGG CTCAGTGGTC 
AATGTGAACC CCACGAACAC TAGGCCACAG AGTGATACCC CCGAGATTCG TAAATACAAG 
AAGAGATTTA ACTCAGAGAT CCTGTGTGCT GCCTTATGGG GAGTGAATTT GCTAGTTGGC 
ACAGAGAGTG GCCTGATGCT CTTGGACAGA AGTGGCCAAG GGAAAGTGTA CCCTCTGATC 
AGCCGGAGAC GGTTCCAGCA AATGGATGTA CTTGAGGGCC TGAATGTCCT GGTGACAATA 
TCTGGCAAAA AGGATAAGTT ACGCGTCTAC TATTTATCCT GGTTAAGAAA TAAAATACTT 
CATAATGATC CGGAAGTTGA GAAGAAGCAG GGGTGGACCA CCGTGGGCGA CCTGGAAGGG 
TGCGTGCACT ATAAAGTCGT AAAATATGAA AGAATCAAGT TTCTGGTAAT CGCTTTGAAG 
AGTTCTGTGG AAGTCTATGC ATGGGCACCG AAGCCATATC ACAAATTTAT GGCCTTTAAG 
TCATTTGGAG AACTGGTACA TAAGCCATTA CTGGTGGATC TCACTGTCGA GGAAGGCCAG 
AGGTTGAAAG TGATCTATGG ATCCTGTGCT GGATTCCATG CTGTTGATGT GGATTCCGGC 
TCAGTCTATG ACATTTATCT ACCAACACAC ATTCAGTGTA CCATCAAACC CCATGCAATC 
ATTATCCTCC CCAACACGGA TGGAATGGAG CTTCTGGTGT GCTACGAAGA TGAAGGGGTT 
TATGTGAACA CGTACGGAAG AATCACCAAG GACGTGGTTC TGCAGTGGGG AGAGATGCCA 
ACATCTGTAG CATACATCCG GTCGAATCAG ACAATGGGCT GGGGAGAGAA GGCAATAGAG 
ATACGCTCTG TGGAAACTGG TCATTTGGAC GGTGTGTTTA TGCACAAAAG GGCTCAGAGA 
CTAAAGTTCC TGTGTGAACG TAATGACAAG GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC 
AGCAGCCAGG TCTACTTCAT GACCTTAGGC AGGACTTCCC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_008767013.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765235.2 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X18, mRNA.

Price & Availability↑

CloneID	ORa62707
Clone ID Related Accession (Same CDS sequence)	XM_008767013.2
Accession Version	XM_008767013.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767013.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTTCC TGTGAGAACG 
ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA 
AATAGCCAAG CAGGACAGAG AAATTCCACC AGCAGTATTG AGCCCCGGCT TCTATGGGAG 
AGAGTGGAAA AGCTGGTCCC CAGGCCAGGC AGTGGCAGCT CTTCAGGGTC CAGCAACTCA 
GGATCCCAGC CTGGCTCCCA TCCTGGGTCT CAGAGTGGCT CTGGTGAACG CTTCAGAGTG 
AGATCATCAT CCAAGTCTGA AGGTTCTCCA TCTCAGCGCC TTGAAAATGC AGCCAAAAAA 
CCTGAAGATA AGAAAGAAGT ATTTCGACCT CTCAAACCTG CTGGAGAAGT GGACTTGACT 
GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTGCGGC CACCTCACAA AGTGACAGAC 
TATTCCTCCT CCAGTGAGGA GTCTGGGACC ACGGATGAGG AGGAGGAGGA TGTGGAACAG 
GAGGGGGCTG ATGATTCCAC CTCGGGGCCA GAGGACACCA GAGCAGCGTC GTCTCTGAAT 
CTGAGCAACG GTGAGACAGA ATCTGTGAAA ACAATGATTG TTCATGATGA CGTAGAAAGT 
GAACCAGCCA TGACCCCATC CAAGGAGGGC ACCCTAATCG TCCGCCAGAG TACAGTTGAC 
CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA 
GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCTTCCTC CCCATCCTCC 
AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG 
ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
ATAGACCCCA GGTTACTACA GATCTCTCCA TCTAGTGGAA CAACAGTTAC TTCCGTTGTG 
GGATTTTCCT GTGATGGGCT GAGACCAGAA GCCATAAGGC AAGATCCTAC TCGGAAAGGC 
TCAGTGGTCA ATGTGAACCC CACGAACACT AGGCCACAGA GTGATACCCC CGAGATTCGT 
AAATACAAGA AGAGATTTAA CTCAGAGATC CTGTGTGCTG CCTTATGGGG AGTGAATTTG 
CTAGTTGGCA CAGAGAGTGG CCTGATGCTC TTGGACAGAA GTGGCCAAGG GAAAGTGTAC 
CCTCTGATCA GCCGGAGACG GTTCCAGCAA ATGGATGTAC TTGAGGGCCT GAATGTCCTG 
GTGACAATAT CTGGCAAAAA GGATAAGTTA CGCGTCTACT ATTTATCCTG GTTAAGAAAT 
AAAATACTTC ATAATGATCC GGAAGTTGAG AAGAAGCAGG GGTGGACCAC CGTGGGCGAC 
CTGGAAGGGT GCGTGCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTAATC 
GCTTTGAAGA GTTCTGTGGA AGTCTATGCA TGGGCACCGA AGCCATATCA CAAATTTATG 
GCCTTTAAGT CATTTGGAGA ACTGGTACAT AAGCCATTAC TGGTGGATCT CACTGTCGAG 
GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG 
GATTCCGGCT CAGTCTATGA CATTTATCTA CCAACACACG TAAGAAAGAA CCCACACTCT 
ATGATTCAGT GTACCATCAA ACCCCATGCA ATCATTATCC TCCCCAACAC GGATGGAATG 
GAGCTTCTGG TGTGCTACGA AGATGAAGGG GTTTATGTGA ACACGTACGG AAGAATCACC 
AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCAACATCTG TAGCATACAT CCGGTCGAAT 
CAGACAATGG GCTGGGGAGA GAAGGCAATA GAGATACGCT CTGTGGAAAC TGGTCATTTG 
GACGGTGTGT TTATGCACAA AAGGGCTCAG AGACTAAAGT TCCTGTGTGA ACGTAATGAC 
AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC GGCAGCAGCC AGGTCTACTT CATGACCTTA 
GGCAGGACTT CCCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765235.2
CDS	209..3895
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008767013.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767013.2

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTTCC TGTGAGAACG 
ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA 
AATAGCCAAG CAGGACAGAG AAATTCCACC AGCAGTATTG AGCCCCGGCT TCTATGGGAG 
AGAGTGGAAA AGCTGGTCCC CAGGCCAGGC AGTGGCAGCT CTTCAGGGTC CAGCAACTCA 
GGATCCCAGC CTGGCTCCCA TCCTGGGTCT CAGAGTGGCT CTGGTGAACG CTTCAGAGTG 
AGATCATCAT CCAAGTCTGA AGGTTCTCCA TCTCAGCGCC TTGAAAATGC AGCCAAAAAA 
CCTGAAGATA AGAAAGAAGT ATTTCGACCT CTCAAACCTG CTGGAGAAGT GGACTTGACT 
GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTGCGGC CACCTCACAA AGTGACAGAC 
TATTCCTCCT CCAGTGAGGA GTCTGGGACC ACGGATGAGG AGGAGGAGGA TGTGGAACAG 
GAGGGGGCTG ATGATTCCAC CTCGGGGCCA GAGGACACCA GAGCAGCGTC GTCTCTGAAT 
CTGAGCAACG GTGAGACAGA ATCTGTGAAA ACAATGATTG TTCATGATGA CGTAGAAAGT 
GAACCAGCCA TGACCCCATC CAAGGAGGGC ACCCTAATCG TCCGCCAGAG TACAGTTGAC 
CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA 
GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCTTCCTC CCCATCCTCC 
AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG 
ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
ATAGACCCCA GGTTACTACA GATCTCTCCA TCTAGTGGAA CAACAGTTAC TTCCGTTGTG 
GGATTTTCCT GTGATGGGCT GAGACCAGAA GCCATAAGGC AAGATCCTAC TCGGAAAGGC 
TCAGTGGTCA ATGTGAACCC CACGAACACT AGGCCACAGA GTGATACCCC CGAGATTCGT 
AAATACAAGA AGAGATTTAA CTCAGAGATC CTGTGTGCTG CCTTATGGGG AGTGAATTTG 
CTAGTTGGCA CAGAGAGTGG CCTGATGCTC TTGGACAGAA GTGGCCAAGG GAAAGTGTAC 
CCTCTGATCA GCCGGAGACG GTTCCAGCAA ATGGATGTAC TTGAGGGCCT GAATGTCCTG 
GTGACAATAT CTGGCAAAAA GGATAAGTTA CGCGTCTACT ATTTATCCTG GTTAAGAAAT 
AAAATACTTC ATAATGATCC GGAAGTTGAG AAGAAGCAGG GGTGGACCAC CGTGGGCGAC 
CTGGAAGGGT GCGTGCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTAATC 
GCTTTGAAGA GTTCTGTGGA AGTCTATGCA TGGGCACCGA AGCCATATCA CAAATTTATG 
GCCTTTAAGT CATTTGGAGA ACTGGTACAT AAGCCATTAC TGGTGGATCT CACTGTCGAG 
GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG 
GATTCCGGCT CAGTCTATGA CATTTATCTA CCAACACACG TAAGAAAGAA CCCACACTCT 
ATGATTCAGT GTACCATCAA ACCCCATGCA ATCATTATCC TCCCCAACAC GGATGGAATG 
GAGCTTCTGG TGTGCTACGA AGATGAAGGG GTTTATGTGA ACACGTACGG AAGAATCACC 
AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCAACATCTG TAGCATACAT CCGGTCGAAT 
CAGACAATGG GCTGGGGAGA GAAGGCAATA GAGATACGCT CTGTGGAAAC TGGTCATTTG 
GACGGTGTGT TTATGCACAA AAGGGCTCAG AGACTAAAGT TCCTGTGTGA ACGTAATGAC 
AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC GGCAGCAGCC AGGTCTACTT CATGACCTTA 
GGCAGGACTT CCCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_017596344.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451833.1 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X19, mRNA.

Price & Availability↑

CloneID	ORa62708
Clone ID Related Accession (Same CDS sequence)	XM_017596344.1
Accession Version	XM_017596344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTTCC TGTGAGAACG 
ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA 
AATAGCCAAG CAGGACAGAG AAATTCCACC AGTATTGAGC CCCGGCTTCT ATGGGAGAGA 
GTGGAAAAGC TGGTCCCCAG GCCAGGCAGT GGCAGCTCTT CAGGGTCCAG CAACTCAGGA 
TCCCAGCCTG GCTCCCATCC TGGGTCTCAG AGTGGCTCTG GTGAACGCTT CAGAGTGAGA 
TCATCATCCA AGTCTGAAGG TTCTCCATCT CAGCGCCTTG AAAATGCAGC CAAAAAACCT 
GAAGATAAGA AAGAAGTATT TCGACCTCTC AAACCTGCTG GAGAAGTGGA CTTGACTGCG 
TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTGCGGCCAC CTCACAAAGT GACAGACTAT 
TCCTCCTCCA GTGAGGAGTC TGGGACCACG GATGAGGAGG AGGAGGATGT GGAACAGGAG 
GGGGCTGATG ATTCCACCTC GGGGCCAGAG GACACCAGAG CAGCGTCGTC TCTGAATCTG 
AGCAACGGTG AGACAGAATC TGTGAAAACA ATGATTGTTC ATGATGACGT AGAAAGTGAA 
CCAGCCATGA CCCCATCCAA GGAGGGCACC CTAATCGTCC GCCAGAGTAC AGTTGACCAA 
AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCGGGTC GCATTCACCT CTTGCCAGAT 
CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CTTCCTCCCC ATCCTCCAGC 
CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT 
CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
GACCCCAGGT TACTACAGAT CTCTCCATCT AGTGGAACAA CAGTTACTTC CGTTGTGGGA 
TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC ATAAGGCAAG ATCCTACTCG GAAAGGCTCA 
GTGGTCAATG TGAACCCCAC GAACACTAGG CCACAGAGTG ATACCCCCGA GATTCGTAAA 
TACAAGAAGA GATTTAACTC AGAGATCCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA 
GTTGGCACAG AGAGTGGCCT GATGCTCTTG GACAGAAGTG GCCAAGGGAA AGTGTACCCT 
CTGATCAGCC GGAGACGGTT CCAGCAAATG GATGTACTTG AGGGCCTGAA TGTCCTGGTG 
ACAATATCTG GCAAAAAGGA TAAGTTACGC GTCTACTATT TATCCTGGTT AAGAAATAAA 
ATACTTCATA ATGATCCGGA AGTTGAGAAG AAGCAGGGGT GGACCACCGT GGGCGACCTG 
GAAGGGTGCG TGCACTATAA AGTCGTAAAA TATGAAAGAA TCAAGTTTCT GGTAATCGCT 
TTGAAGAGTT CTGTGGAAGT CTATGCATGG GCACCGAAGC CATATCACAA ATTTATGGCC 
TTTAAGTCAT TTGGAGAACT GGTACATAAG CCATTACTGG TGGATCTCAC TGTCGAGGAA 
GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT 
TCCGGCTCAG TCTATGACAT TTATCTACCA ACACACGTAA GAAAGAACCC ACACTCTATG 
ATTCAGTGTA CCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGAATGGAG 
CTTCTGGTGT GCTACGAAGA TGAAGGGGTT TATGTGAACA CGTACGGAAG AATCACCAAG 
GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATACATCCG GTCGAATCAG 
ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGCTCTG TGGAAACTGG TCATTTGGAC 
GGTGTGTTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG TAATGACAAG 
GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC AGCAGCCAGG TCTACTTCAT GACCTTAGGC 
AGGACTTCCC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451833.1
CDS	209..3892
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017596344.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596344.1

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTTCC TGTGAGAACG 
ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA 
AATAGCCAAG CAGGACAGAG AAATTCCACC AGTATTGAGC CCCGGCTTCT ATGGGAGAGA 
GTGGAAAAGC TGGTCCCCAG GCCAGGCAGT GGCAGCTCTT CAGGGTCCAG CAACTCAGGA 
TCCCAGCCTG GCTCCCATCC TGGGTCTCAG AGTGGCTCTG GTGAACGCTT CAGAGTGAGA 
TCATCATCCA AGTCTGAAGG TTCTCCATCT CAGCGCCTTG AAAATGCAGC CAAAAAACCT 
GAAGATAAGA AAGAAGTATT TCGACCTCTC AAACCTGCTG GAGAAGTGGA CTTGACTGCG 
TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTGCGGCCAC CTCACAAAGT GACAGACTAT 
TCCTCCTCCA GTGAGGAGTC TGGGACCACG GATGAGGAGG AGGAGGATGT GGAACAGGAG 
GGGGCTGATG ATTCCACCTC GGGGCCAGAG GACACCAGAG CAGCGTCGTC TCTGAATCTG 
AGCAACGGTG AGACAGAATC TGTGAAAACA ATGATTGTTC ATGATGACGT AGAAAGTGAA 
CCAGCCATGA CCCCATCCAA GGAGGGCACC CTAATCGTCC GCCAGAGTAC AGTTGACCAA 
AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCGGGTC GCATTCACCT CTTGCCAGAT 
CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CTTCCTCCCC ATCCTCCAGC 
CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT 
CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
GACCCCAGGT TACTACAGAT CTCTCCATCT AGTGGAACAA CAGTTACTTC CGTTGTGGGA 
TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC ATAAGGCAAG ATCCTACTCG GAAAGGCTCA 
GTGGTCAATG TGAACCCCAC GAACACTAGG CCACAGAGTG ATACCCCCGA GATTCGTAAA 
TACAAGAAGA GATTTAACTC AGAGATCCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA 
GTTGGCACAG AGAGTGGCCT GATGCTCTTG GACAGAAGTG GCCAAGGGAA AGTGTACCCT 
CTGATCAGCC GGAGACGGTT CCAGCAAATG GATGTACTTG AGGGCCTGAA TGTCCTGGTG 
ACAATATCTG GCAAAAAGGA TAAGTTACGC GTCTACTATT TATCCTGGTT AAGAAATAAA 
ATACTTCATA ATGATCCGGA AGTTGAGAAG AAGCAGGGGT GGACCACCGT GGGCGACCTG 
GAAGGGTGCG TGCACTATAA AGTCGTAAAA TATGAAAGAA TCAAGTTTCT GGTAATCGCT 
TTGAAGAGTT CTGTGGAAGT CTATGCATGG GCACCGAAGC CATATCACAA ATTTATGGCC 
TTTAAGTCAT TTGGAGAACT GGTACATAAG CCATTACTGG TGGATCTCAC TGTCGAGGAA 
GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT 
TCCGGCTCAG TCTATGACAT TTATCTACCA ACACACGTAA GAAAGAACCC ACACTCTATG 
ATTCAGTGTA CCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGAATGGAG 
CTTCTGGTGT GCTACGAAGA TGAAGGGGTT TATGTGAACA CGTACGGAAG AATCACCAAG 
GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATACATCCG GTCGAATCAG 
ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGCTCTG TGGAAACTGG TCATTTGGAC 
GGTGTGTTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG TAATGACAAG 
GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC AGCAGCCAGG TCTACTTCAT GACCTTAGGC 
AGGACTTCCC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_008767014.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765236.2 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X20, mRNA.

Price & Availability↑

CloneID	ORa62709
Clone ID Related Accession (Same CDS sequence)	XM_008767014.2
Accession Version	XM_008767014.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767014.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT 
AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG 
CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT 
GCCCTGCAGA GACCAGCGGA GCCACAGGTT CCTGTGAGAA CGACATCTCG TTCCCCTGTC 
CTGTCCCGTC GAGATTCTCC ACTGCAGGGC AGTGGGCAGC AAAATAGCCA AGCAGGACAG 
AGAAATTCCA CCAGCAGTAT TGAGCCCCGG CTTCTATGGG AGAGAGTGGA AAAGCTGGTC 
CCCAGGCCAG GCAGTGGCAG CTCTTCAGGG TCCAGCAACT CAGGATCCCA GCCTGGCTCC 
CATCCTGGGT CTCAGAGTGG CTCTGGTGAA CGCTTCAGAG TGAGATCATC ATCCAAGTCT 
GAAGGTTCTC CATCTCAGCG CCTTGAAAAT GCAGCCAAAA AACCTGAAGA TAAGAAAGAA 
GTATTTCGAC CTCTCAAACC TGCTGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG 
GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG 
ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG 
CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG 
AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG 
GGCACCCTAA TCGTCCGCCA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT 
TCCTCCTCCT TTACACCTTT TATAGACCCC AGGTTACTAC AGATCTCTCC ATCTAGTGGA 
ACAACAGTTA CTTCCGTTGT GGGATTTTCC TGTGATGGGC TGAGACCAGA AGCCATAAGG 
CAAGATCCTA CTCGGAAAGG CTCAGTGGTC AATGTGAACC CCACGAACAC TAGGCCACAG 
AGTGATACCC CCGAGATTCG TAAATACAAG AAGAGATTTA ACTCAGAGAT CCTGTGTGCT 
GCCTTATGGG GAGTGAATTT GCTAGTTGGC ACAGAGAGTG GCCTGATGCT CTTGGACAGA 
AGTGGCCAAG GGAAAGTGTA CCCTCTGATC AGCCGGAGAC GGTTCCAGCA AATGGATGTA 
CTTGAGGGCC TGAATGTCCT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGCGTCTAC 
TATTTATCCT GGTTAAGAAA TAAAATACTT CATAATGATC CGGAAGTTGA GAAGAAGCAG 
GGGTGGACCA CCGTGGGCGA CCTGGAAGGG TGCGTGCACT ATAAAGTCGT AAAATATGAA 
AGAATCAAGT TTCTGGTAAT CGCTTTGAAG AGTTCTGTGG AAGTCTATGC ATGGGCACCG 
AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AACTGGTACA TAAGCCATTA 
CTGGTGGATC TCACTGTCGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT 
GGATTCCATG CTGTTGATGT GGATTCCGGC TCAGTCTATG ACATTTATCT ACCAACACAC 
ATTCAGTGTA CCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGAATGGAG 
CTTCTGGTGT GCTACGAAGA TGAAGGGGTT TATGTGAACA CGTACGGAAG AATCACCAAG 
GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATACATCCG GTCGAATCAG 
ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGCTCTG TGGAAACTGG TCATTTGGAC 
GGTGTGTTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG TAATGACAAG 
GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC AGCAGCCAGG TCTACTTCAT GACCTTAGGC 
AGGACTTCCC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765236.2
CDS	208..3831
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVEDRFRKTNHSSPEAQAKQTGRGLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008767014.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767014.2

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT 
AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG 
CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT 
GCCCTGCAGA GACCAGCGGA GCCACAGGTT CCTGTGAGAA CGACATCTCG TTCCCCTGTC 
CTGTCCCGTC GAGATTCTCC ACTGCAGGGC AGTGGGCAGC AAAATAGCCA AGCAGGACAG 
AGAAATTCCA CCAGCAGTAT TGAGCCCCGG CTTCTATGGG AGAGAGTGGA AAAGCTGGTC 
CCCAGGCCAG GCAGTGGCAG CTCTTCAGGG TCCAGCAACT CAGGATCCCA GCCTGGCTCC 
CATCCTGGGT CTCAGAGTGG CTCTGGTGAA CGCTTCAGAG TGAGATCATC ATCCAAGTCT 
GAAGGTTCTC CATCTCAGCG CCTTGAAAAT GCAGCCAAAA AACCTGAAGA TAAGAAAGAA 
GTATTTCGAC CTCTCAAACC TGCTGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG 
GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG 
ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG 
CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG 
AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG 
GGCACCCTAA TCGTCCGCCA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT 
TCCTCCTCCT TTACACCTTT TATAGACCCC AGGTTACTAC AGATCTCTCC ATCTAGTGGA 
ACAACAGTTA CTTCCGTTGT GGGATTTTCC TGTGATGGGC TGAGACCAGA AGCCATAAGG 
CAAGATCCTA CTCGGAAAGG CTCAGTGGTC AATGTGAACC CCACGAACAC TAGGCCACAG 
AGTGATACCC CCGAGATTCG TAAATACAAG AAGAGATTTA ACTCAGAGAT CCTGTGTGCT 
GCCTTATGGG GAGTGAATTT GCTAGTTGGC ACAGAGAGTG GCCTGATGCT CTTGGACAGA 
AGTGGCCAAG GGAAAGTGTA CCCTCTGATC AGCCGGAGAC GGTTCCAGCA AATGGATGTA 
CTTGAGGGCC TGAATGTCCT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGCGTCTAC 
TATTTATCCT GGTTAAGAAA TAAAATACTT CATAATGATC CGGAAGTTGA GAAGAAGCAG 
GGGTGGACCA CCGTGGGCGA CCTGGAAGGG TGCGTGCACT ATAAAGTCGT AAAATATGAA 
AGAATCAAGT TTCTGGTAAT CGCTTTGAAG AGTTCTGTGG AAGTCTATGC ATGGGCACCG 
AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AACTGGTACA TAAGCCATTA 
CTGGTGGATC TCACTGTCGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT 
GGATTCCATG CTGTTGATGT GGATTCCGGC TCAGTCTATG ACATTTATCT ACCAACACAC 
ATTCAGTGTA CCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGAATGGAG 
CTTCTGGTGT GCTACGAAGA TGAAGGGGTT TATGTGAACA CGTACGGAAG AATCACCAAG 
GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATACATCCG GTCGAATCAG 
ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGCTCTG TGGAAACTGG TCATTTGGAC 
GGTGTGTTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG TAATGACAAG 
GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC AGCAGCCAGG TCTACTTCAT GACCTTAGGC 
AGGACTTCCC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_017596345.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451834.1 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X21, mRNA.

Price & Availability↑

CloneID	ORa62710
Clone ID Related Accession (Same CDS sequence)	XM_017596345.1
Accession Version	XM_017596345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT 
AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG 
CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT 
GCCCTGCAGA GACCAGCGGA GCCACAGGTA CAGTGGTCCC ACCTGGCATC TCTCAAGAAC 
AATGTCTCCC CTGTCTCGAG ATCCCATTCC TTCAGTGACC CTTCTCCCAA ATTCGCACAC 
CACCATCTCC GCTCTCAGGA CCCATGTCCA CCTTCCCGCA GTGAGGGGCT CAGTCAGAGC 
TCTGACTCTA AGTCGGAGGT ACCTGAGCCC ACCCAAAAGG CTTGGTCTAG ATCAGACAGT 
GACGAGGTGC CTCCAAGGGT TCCTGTGAGA ACGACATCTC GTTCCCCTGT CCTGTCCCGT 
CGAGATTCTC CACTGCAGGG CAGTGGGCAG CAAAATAGCC AAGCAGGACA GAGAAATTCC 
ACCAGCAGTA TTGAGCCCCG GCTTCTATGG GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA 
GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC TCAGGATCCC AGCCTGGCTC CCATCCTGGG 
TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA GTGAGATCAT CATCCAAGTC TGAAGGTTCT 
CCATCTCAGC GCCTTGAAAA TGCAGCCAAA AAACCTGAAG ATAAGAAAGA AGTATTTCGA 
CCTCTCAAAC CTGCTGGAGA AGTGGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG 
GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG 
ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG 
CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG 
AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG 
GGCACCCTAA TCGTCCGCCA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT 
TCCTCCTCCT TTACACCTTT TATAGACCCC AGGTTACTAC AGATCTCTCC ATCTAGTGGA 
ACAACAGTTA CTTCCGTTGT GGGATTTTCC TGTGATGGGC TGAGACCAGA AGCCATAAGG 
CAAGATCCTA CTCGGAAAGG CTCAGTGGTC AATGTGAACC CCACGAACAC TAGGCCACAG 
AGTGATACCC CCGAGATTCG TAAATACAAG AAGAGATTTA ACTCAGAGAT CCTGTGTGCT 
GCCTTATGGG GAGTGAATTT GCTAGTTGGC ACAGAGAGTG GCCTGATGCT CTTGGACAGA 
AGTGGCCAAG GGAAAGTGTA CCCTCTGATC AGCCGGAGAC GGTTCCAGCA AATGGATGTA 
CTTGAGGGCC TGAATGTCCT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGCGTCTAC 
TATTTATCCT GGTTAAGAAA TAAAATACTT CATAATGATC CGGAAGTTGA GAAGAAGCAG 
GGGTGGACCA CCGTGGGCGA CCTGGAAGGG TGCGTGCACT ATAAAGTCGT AAAATATGAA 
AGAATCAAGT TTCTGGTAAT CGCTTTGAAG AGTTCTGTGG AAGTCTATGC ATGGGCACCG 
AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AACTGGTACA TAAGCCATTA 
CTGGTGGATC TCACTGTCGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT 
GGATTCCATG CTGTTGATGT GGATTCCGGC TCAGTCTATG ACATTTATCT ACCAACACAC 
ATTCAGTGTA CCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGAATGGAG 
CTTCTGGTGT GCTACGAAGA TGAAGGGGTT TATGTGAACA CGTACGGAAG AATCACCAAG 
GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATACATCCG GTCGAATCAG 
ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGCTCTG TGGAAACTGG TCATTTGGAC 
GGTGTGTTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG TAATGACAAG 
GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC AGCAGCCAGG TCTACTTCAT GACCTTAGGC 
AGGACTTCCC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451834.1
CDS	210..4073
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVEDRFRKTNHSSPEAQAKQTGRGLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017596345.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596345.1

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT 
AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG 
CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT 
GCCCTGCAGA GACCAGCGGA GCCACAGGTA CAGTGGTCCC ACCTGGCATC TCTCAAGAAC 
AATGTCTCCC CTGTCTCGAG ATCCCATTCC TTCAGTGACC CTTCTCCCAA ATTCGCACAC 
CACCATCTCC GCTCTCAGGA CCCATGTCCA CCTTCCCGCA GTGAGGGGCT CAGTCAGAGC 
TCTGACTCTA AGTCGGAGGT ACCTGAGCCC ACCCAAAAGG CTTGGTCTAG ATCAGACAGT 
GACGAGGTGC CTCCAAGGGT TCCTGTGAGA ACGACATCTC GTTCCCCTGT CCTGTCCCGT 
CGAGATTCTC CACTGCAGGG CAGTGGGCAG CAAAATAGCC AAGCAGGACA GAGAAATTCC 
ACCAGCAGTA TTGAGCCCCG GCTTCTATGG GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA 
GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC TCAGGATCCC AGCCTGGCTC CCATCCTGGG 
TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA GTGAGATCAT CATCCAAGTC TGAAGGTTCT 
CCATCTCAGC GCCTTGAAAA TGCAGCCAAA AAACCTGAAG ATAAGAAAGA AGTATTTCGA 
CCTCTCAAAC CTGCTGGAGA AGTGGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG 
GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG 
ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG 
CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG 
AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG 
GGCACCCTAA TCGTCCGCCA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT 
TCCTCCTCCT TTACACCTTT TATAGACCCC AGGTTACTAC AGATCTCTCC ATCTAGTGGA 
ACAACAGTTA CTTCCGTTGT GGGATTTTCC TGTGATGGGC TGAGACCAGA AGCCATAAGG 
CAAGATCCTA CTCGGAAAGG CTCAGTGGTC AATGTGAACC CCACGAACAC TAGGCCACAG 
AGTGATACCC CCGAGATTCG TAAATACAAG AAGAGATTTA ACTCAGAGAT CCTGTGTGCT 
GCCTTATGGG GAGTGAATTT GCTAGTTGGC ACAGAGAGTG GCCTGATGCT CTTGGACAGA 
AGTGGCCAAG GGAAAGTGTA CCCTCTGATC AGCCGGAGAC GGTTCCAGCA AATGGATGTA 
CTTGAGGGCC TGAATGTCCT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGCGTCTAC 
TATTTATCCT GGTTAAGAAA TAAAATACTT CATAATGATC CGGAAGTTGA GAAGAAGCAG 
GGGTGGACCA CCGTGGGCGA CCTGGAAGGG TGCGTGCACT ATAAAGTCGT AAAATATGAA 
AGAATCAAGT TTCTGGTAAT CGCTTTGAAG AGTTCTGTGG AAGTCTATGC ATGGGCACCG 
AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AACTGGTACA TAAGCCATTA 
CTGGTGGATC TCACTGTCGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT 
GGATTCCATG CTGTTGATGT GGATTCCGGC TCAGTCTATG ACATTTATCT ACCAACACAC 
ATTCAGTGTA CCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGAATGGAG 
CTTCTGGTGT GCTACGAAGA TGAAGGGGTT TATGTGAACA CGTACGGAAG AATCACCAAG 
GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATACATCCG GTCGAATCAG 
ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGCTCTG TGGAAACTGG TCATTTGGAC 
GGTGTGTTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG TAATGACAAG 
GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC AGCAGCCAGG TCTACTTCAT GACCTTAGGC 
AGGACTTCCC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_008767015.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765237.2 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X22, mRNA.

Price & Availability↑

CloneID	ORa62711
Clone ID Related Accession (Same CDS sequence)	XM_008767015.2
Accession Version	XM_008767015.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767015.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTACA GTGGTCCCAC 
CTGGCATCTC TCAAGAACAA TGTCTCCCCT GTCTCGAGAT CCCATTCCTT CAGTGACCCT 
TCTCCCAAAT TCGCACACCA CCATCTCCGC TCTCAGGACC CATGTCCACC TTCCCGCAGT 
GAGGGGCTCA GTCAGAGCTC TGACTCTAAG TCGGAGGTAC CTGAGCCCAC CCAAAAGGCT 
TGGTCTAGAT CAGACAGTGA CGAGGTGCCT CCAAGGGTTC CTGTGAGAAC GACATCTCGT 
TCCCCTGTCC TGTCCCGTCG AGATTCTCCA CTGCAGGGCA GTGGGCAGCA AAATAGCCAA 
GCAGGACAGA GAAATTCCAC CAGCAGTATT GAGCCCCGGC TTCTATGGGA GAGAGTGGAA 
AAGCTGGTCC CCAGGCCAGG CAGTGGCAGC TCTTCAGGGT CCAGCAACTC AGGATCCCAG 
CCTGGCTCCC ATCCTGGGTC TCAGAGTGGC TCTGGTGAAC GCTTCAGAGT GAGATCATCA 
TCCAAGTCTG AAGGTTCTCC ATCTCAGCGC CTTGAAAATG CAGCCAAAAA ACCTGAAGAT 
AAGAAAGAAG TATTTCGACC TCTCAAACCT GCTGGAGAAG TGGACTTGAC TGCGTTGGCC 
AAAGAGCTTC GAGCAGTGGA AGATGTGCGG CCACCTCACA AAGTGACAGA CTATTCCTCC 
TCCAGTGAGG AGTCTGGGAC CACGGATGAG GAGGAGGAGG ATGTGGAACA GGAGGGGGCT 
GATGATTCCA CCTCGGGGCC AGAGGACACC AGAGCAGCGT CGTCTCTGAA TCTGAGCAAC 
GGTGAGACAG AATCTGTGAA AACAATGATT GTTCATGATG ACGTAGAAAG TGAACCAGCC 
ATGACCCCAT CCAAGGAGGG CACCCTAATC GTCCGCCAGA CTCAGTCCGC TAGTAGCACA 
CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG GTTACTACAG 
ATCTCTCCAT CTAGTGGAAC AACAGTTACT TCCGTTGTGG GATTTTCCTG TGATGGGCTG 
AGACCAGAAG CCATAAGGCA AGATCCTACT CGGAAAGGCT CAGTGGTCAA TGTGAACCCC 
ACGAACACTA GGCCACAGAG TGATACCCCC GAGATTCGTA AATACAAGAA GAGATTTAAC 
TCAGAGATCC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTTGGCAC AGAGAGTGGC 
CTGATGCTCT TGGACAGAAG TGGCCAAGGG AAAGTGTACC CTCTGATCAG CCGGAGACGG 
TTCCAGCAAA TGGATGTACT TGAGGGCCTG AATGTCCTGG TGACAATATC TGGCAAAAAG 
GATAAGTTAC GCGTCTACTA TTTATCCTGG TTAAGAAATA AAATACTTCA TAATGATCCG 
GAAGTTGAGA AGAAGCAGGG GTGGACCACC GTGGGCGACC TGGAAGGGTG CGTGCACTAT 
AAAGTCGTAA AATATGAAAG AATCAAGTTT CTGGTAATCG CTTTGAAGAG TTCTGTGGAA 
GTCTATGCAT GGGCACCGAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA 
CTGGTACATA AGCCATTACT GGTGGATCTC ACTGTCGAGG AAGGCCAGAG GTTGAAAGTG 
ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCCGGCTC AGTCTATGAC 
ATTTATCTAC CAACACACAT TCAGTGTACC ATCAAACCCC ATGCAATCAT TATCCTCCCC 
AACACGGATG GAATGGAGCT TCTGGTGTGC TACGAAGATG AAGGGGTTTA TGTGAACACG 
TACGGAAGAA TCACCAAGGA CGTGGTTCTG CAGTGGGGAG AGATGCCAAC ATCTGTAGCA 
TACATCCGGT CGAATCAGAC AATGGGCTGG GGAGAGAAGG CAATAGAGAT ACGCTCTGTG 
GAAACTGGTC ATTTGGACGG TGTGTTTATG CACAAAAGGG CTCAGAGACT AAAGTTCCTG 
TGTGAACGTA ATGACAAGGT GTTCTTTGCT TCCGTCCGCT CTGGCGGCAG CAGCCAGGTC 
TACTTCATGA CCTTAGGCAG GACTTCCCTT CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765237.2
CDS	209..3910
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008767015.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767015.2

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTACA GTGGTCCCAC 
CTGGCATCTC TCAAGAACAA TGTCTCCCCT GTCTCGAGAT CCCATTCCTT CAGTGACCCT 
TCTCCCAAAT TCGCACACCA CCATCTCCGC TCTCAGGACC CATGTCCACC TTCCCGCAGT 
GAGGGGCTCA GTCAGAGCTC TGACTCTAAG TCGGAGGTAC CTGAGCCCAC CCAAAAGGCT 
TGGTCTAGAT CAGACAGTGA CGAGGTGCCT CCAAGGGTTC CTGTGAGAAC GACATCTCGT 
TCCCCTGTCC TGTCCCGTCG AGATTCTCCA CTGCAGGGCA GTGGGCAGCA AAATAGCCAA 
GCAGGACAGA GAAATTCCAC CAGCAGTATT GAGCCCCGGC TTCTATGGGA GAGAGTGGAA 
AAGCTGGTCC CCAGGCCAGG CAGTGGCAGC TCTTCAGGGT CCAGCAACTC AGGATCCCAG 
CCTGGCTCCC ATCCTGGGTC TCAGAGTGGC TCTGGTGAAC GCTTCAGAGT GAGATCATCA 
TCCAAGTCTG AAGGTTCTCC ATCTCAGCGC CTTGAAAATG CAGCCAAAAA ACCTGAAGAT 
AAGAAAGAAG TATTTCGACC TCTCAAACCT GCTGGAGAAG TGGACTTGAC TGCGTTGGCC 
AAAGAGCTTC GAGCAGTGGA AGATGTGCGG CCACCTCACA AAGTGACAGA CTATTCCTCC 
TCCAGTGAGG AGTCTGGGAC CACGGATGAG GAGGAGGAGG ATGTGGAACA GGAGGGGGCT 
GATGATTCCA CCTCGGGGCC AGAGGACACC AGAGCAGCGT CGTCTCTGAA TCTGAGCAAC 
GGTGAGACAG AATCTGTGAA AACAATGATT GTTCATGATG ACGTAGAAAG TGAACCAGCC 
ATGACCCCAT CCAAGGAGGG CACCCTAATC GTCCGCCAGA CTCAGTCCGC TAGTAGCACA 
CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG GTTACTACAG 
ATCTCTCCAT CTAGTGGAAC AACAGTTACT TCCGTTGTGG GATTTTCCTG TGATGGGCTG 
AGACCAGAAG CCATAAGGCA AGATCCTACT CGGAAAGGCT CAGTGGTCAA TGTGAACCCC 
ACGAACACTA GGCCACAGAG TGATACCCCC GAGATTCGTA AATACAAGAA GAGATTTAAC 
TCAGAGATCC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTTGGCAC AGAGAGTGGC 
CTGATGCTCT TGGACAGAAG TGGCCAAGGG AAAGTGTACC CTCTGATCAG CCGGAGACGG 
TTCCAGCAAA TGGATGTACT TGAGGGCCTG AATGTCCTGG TGACAATATC TGGCAAAAAG 
GATAAGTTAC GCGTCTACTA TTTATCCTGG TTAAGAAATA AAATACTTCA TAATGATCCG 
GAAGTTGAGA AGAAGCAGGG GTGGACCACC GTGGGCGACC TGGAAGGGTG CGTGCACTAT 
AAAGTCGTAA AATATGAAAG AATCAAGTTT CTGGTAATCG CTTTGAAGAG TTCTGTGGAA 
GTCTATGCAT GGGCACCGAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA 
CTGGTACATA AGCCATTACT GGTGGATCTC ACTGTCGAGG AAGGCCAGAG GTTGAAAGTG 
ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCCGGCTC AGTCTATGAC 
ATTTATCTAC CAACACACAT TCAGTGTACC ATCAAACCCC ATGCAATCAT TATCCTCCCC 
AACACGGATG GAATGGAGCT TCTGGTGTGC TACGAAGATG AAGGGGTTTA TGTGAACACG 
TACGGAAGAA TCACCAAGGA CGTGGTTCTG CAGTGGGGAG AGATGCCAAC ATCTGTAGCA 
TACATCCGGT CGAATCAGAC AATGGGCTGG GGAGAGAAGG CAATAGAGAT ACGCTCTGTG 
GAAACTGGTC ATTTGGACGG TGTGTTTATG CACAAAAGGG CTCAGAGACT AAAGTTCCTG 
TGTGAACGTA ATGACAAGGT GTTCTTTGCT TCCGTCCGCT CTGGCGGCAG CAGCCAGGTC 
TACTTCATGA CCTTAGGCAG GACTTCCCTT CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_008767016.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765238.2 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X23, mRNA.

Price & Availability↑

CloneID	ORa62712
Clone ID Related Accession (Same CDS sequence)	XM_008767016.2
Accession Version	XM_008767016.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767016.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTTCC TGTGAGAACG 
ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA 
AATAGCCAAG CAGGACAGAG AAATTCCACC AGCAGTATTG AGCCCCGGCT TCTATGGGAG 
AGAGTGGAAA AGCTGGTCCC CAGGCCAGGC AGTGGCAGCT CTTCAGGGTC CAGCAACTCA 
GGATCCCAGC CTGGCTCCCA TCCTGGGTCT CAGAGTGGCT CTGGTGAACG CTTCAGAGTG 
AGATCATCAT CCAAGTCTGA AGGTTCTCCA TCTCAGCGCC TTGAAAATGC AGCCAAAAAA 
CCTGAAGATA AGAAAGAAGT ATTTCGACCT CTCAAACCTG CTGGAGAAGT GGACTTGACT 
GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTGCGGC CACCTCACAA AGTGACAGAC 
TATTCCTCCT CCAGTGAGGA GTCTGGGACC ACGGATGAGG AGGAGGAGGA TGTGGAACAG 
GAGGGGGCTG ATGATTCCAC CTCGGGGCCA GAGGACACCA GAGCAGCGTC GTCTCTGAAT 
CTGAGCAACG GTGAGACAGA ATCTGTGAAA ACAATGATTG TTCATGATGA CGTAGAAAGT 
GAACCAGCCA TGACCCCATC CAAGGAGGGC ACCCTAATCG TCCGCCAGAG TACAGTTGAC 
CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA 
GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCTTCCTC CCCATCCTCC 
AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG 
ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
ATAGACCCCA GGTTACTACA GATCTCTCCA TCTAGTGGAA CAACAGTTAC TTCCGTTGTG 
GGATTTTCCT GTGATGGGCT GAGACCAGAA GCCATAAGGC AAGATCCTAC TCGGAAAGGC 
TCAGTGGTCA ATGTGAACCC CACGAACACT AGGCCACAGA GTGATACCCC CGAGATTCGT 
AAATACAAGA AGAGATTTAA CTCAGAGATC CTGTGTGCTG CCTTATGGGG AGTGAATTTG 
CTAGTTGGCA CAGAGAGTGG CCTGATGCTC TTGGACAGAA GTGGCCAAGG GAAAGTGTAC 
CCTCTGATCA GCCGGAGACG GTTCCAGCAA ATGGATGTAC TTGAGGGCCT GAATGTCCTG 
GTGACAATAT CTGGCAAAAA GGATAAGTTA CGCGTCTACT ATTTATCCTG GTTAAGAAAT 
AAAATACTTC ATAATGATCC GGAAGTTGAG AAGAAGCAGG GGTGGACCAC CGTGGGCGAC 
CTGGAAGGGT GCGTGCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTAATC 
GCTTTGAAGA GTTCTGTGGA AGTCTATGCA TGGGCACCGA AGCCATATCA CAAATTTATG 
GCCTTTAAGT CATTTGGAGA ACTGGTACAT AAGCCATTAC TGGTGGATCT CACTGTCGAG 
GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG 
GATTCCGGCT CAGTCTATGA CATTTATCTA CCAACACACA TTCAGTGTAC CATCAAACCC 
CATGCAATCA TTATCCTCCC CAACACGGAT GGAATGGAGC TTCTGGTGTG CTACGAAGAT 
GAAGGGGTTT ATGTGAACAC GTACGGAAGA ATCACCAAGG ACGTGGTTCT GCAGTGGGGA 
GAGATGCCAA CATCTGTAGC ATACATCCGG TCGAATCAGA CAATGGGCTG GGGAGAGAAG 
GCAATAGAGA TACGCTCTGT GGAAACTGGT CATTTGGACG GTGTGTTTAT GCACAAAAGG 
GCTCAGAGAC TAAAGTTCCT GTGTGAACGT AATGACAAGG TGTTCTTTGC TTCCGTCCGC 
TCTGGCGGCA GCAGCCAGGT CTACTTCATG ACCTTAGGCA GGACTTCCCT TCTGAGCTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765238.2
CDS	207..3869
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008767016.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767016.2

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTTCC TGTGAGAACG 
ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA 
AATAGCCAAG CAGGACAGAG AAATTCCACC AGCAGTATTG AGCCCCGGCT TCTATGGGAG 
AGAGTGGAAA AGCTGGTCCC CAGGCCAGGC AGTGGCAGCT CTTCAGGGTC CAGCAACTCA 
GGATCCCAGC CTGGCTCCCA TCCTGGGTCT CAGAGTGGCT CTGGTGAACG CTTCAGAGTG 
AGATCATCAT CCAAGTCTGA AGGTTCTCCA TCTCAGCGCC TTGAAAATGC AGCCAAAAAA 
CCTGAAGATA AGAAAGAAGT ATTTCGACCT CTCAAACCTG CTGGAGAAGT GGACTTGACT 
GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTGCGGC CACCTCACAA AGTGACAGAC 
TATTCCTCCT CCAGTGAGGA GTCTGGGACC ACGGATGAGG AGGAGGAGGA TGTGGAACAG 
GAGGGGGCTG ATGATTCCAC CTCGGGGCCA GAGGACACCA GAGCAGCGTC GTCTCTGAAT 
CTGAGCAACG GTGAGACAGA ATCTGTGAAA ACAATGATTG TTCATGATGA CGTAGAAAGT 
GAACCAGCCA TGACCCCATC CAAGGAGGGC ACCCTAATCG TCCGCCAGAG TACAGTTGAC 
CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA 
GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCTTCCTC CCCATCCTCC 
AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG 
ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
ATAGACCCCA GGTTACTACA GATCTCTCCA TCTAGTGGAA CAACAGTTAC TTCCGTTGTG 
GGATTTTCCT GTGATGGGCT GAGACCAGAA GCCATAAGGC AAGATCCTAC TCGGAAAGGC 
TCAGTGGTCA ATGTGAACCC CACGAACACT AGGCCACAGA GTGATACCCC CGAGATTCGT 
AAATACAAGA AGAGATTTAA CTCAGAGATC CTGTGTGCTG CCTTATGGGG AGTGAATTTG 
CTAGTTGGCA CAGAGAGTGG CCTGATGCTC TTGGACAGAA GTGGCCAAGG GAAAGTGTAC 
CCTCTGATCA GCCGGAGACG GTTCCAGCAA ATGGATGTAC TTGAGGGCCT GAATGTCCTG 
GTGACAATAT CTGGCAAAAA GGATAAGTTA CGCGTCTACT ATTTATCCTG GTTAAGAAAT 
AAAATACTTC ATAATGATCC GGAAGTTGAG AAGAAGCAGG GGTGGACCAC CGTGGGCGAC 
CTGGAAGGGT GCGTGCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTAATC 
GCTTTGAAGA GTTCTGTGGA AGTCTATGCA TGGGCACCGA AGCCATATCA CAAATTTATG 
GCCTTTAAGT CATTTGGAGA ACTGGTACAT AAGCCATTAC TGGTGGATCT CACTGTCGAG 
GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG 
GATTCCGGCT CAGTCTATGA CATTTATCTA CCAACACACA TTCAGTGTAC CATCAAACCC 
CATGCAATCA TTATCCTCCC CAACACGGAT GGAATGGAGC TTCTGGTGTG CTACGAAGAT 
GAAGGGGTTT ATGTGAACAC GTACGGAAGA ATCACCAAGG ACGTGGTTCT GCAGTGGGGA 
GAGATGCCAA CATCTGTAGC ATACATCCGG TCGAATCAGA CAATGGGCTG GGGAGAGAAG 
GCAATAGAGA TACGCTCTGT GGAAACTGGT CATTTGGACG GTGTGTTTAT GCACAAAAGG 
GCTCAGAGAC TAAAGTTCCT GTGTGAACGT AATGACAAGG TGTTCTTTGC TTCCGTCCGC 
TCTGGCGGCA GCAGCCAGGT CTACTTCATG ACCTTAGGCA GGACTTCCCT TCTGAGCTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( XM_017596346.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451835.1 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X24, mRNA.

Price & Availability↑

CloneID	ORa62713
Clone ID Related Accession (Same CDS sequence)	XM_017596346.1
Accession Version	XM_017596346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTTCC TGTGAGAACG 
ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA 
AATAGCCAAG CAGGACAGAG AAATTCCACC AGTATTGAGC CCCGGCTTCT ATGGGAGAGA 
GTGGAAAAGC TGGTCCCCAG GCCAGGCAGT GGCAGCTCTT CAGGGTCCAG CAACTCAGGA 
TCCCAGCCTG GCTCCCATCC TGGGTCTCAG AGTGGCTCTG GTGAACGCTT CAGAGTGAGA 
TCATCATCCA AGTCTGAAGG TTCTCCATCT CAGCGCCTTG AAAATGCAGC CAAAAAACCT 
GAAGATAAGA AAGAAGTATT TCGACCTCTC AAACCTGCTG GAGAAGTGGA CTTGACTGCG 
TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTGCGGCCAC CTCACAAAGT GACAGACTAT 
TCCTCCTCCA GTGAGGAGTC TGGGACCACG GATGAGGAGG AGGAGGATGT GGAACAGGAG 
GGGGCTGATG ATTCCACCTC GGGGCCAGAG GACACCAGAG CAGCGTCGTC TCTGAATCTG 
AGCAACGGTG AGACAGAATC TGTGAAAACA ATGATTGTTC ATGATGACGT AGAAAGTGAA 
CCAGCCATGA CCCCATCCAA GGAGGGCACC CTAATCGTCC GCCAGACTCA GTCCGCTAGT 
AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGGTTA 
CTACAGATCT CTCCATCTAG TGGAACAACA GTTACTTCCG TTGTGGGATT TTCCTGTGAT 
GGGCTGAGAC CAGAAGCCAT AAGGCAAGAT CCTACTCGGA AAGGCTCAGT GGTCAATGTG 
AACCCCACGA ACACTAGGCC ACAGAGTGAT ACCCCCGAGA TTCGTAAATA CAAGAAGAGA 
TTTAACTCAG AGATCCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT TGGCACAGAG 
AGTGGCCTGA TGCTCTTGGA CAGAAGTGGC CAAGGGAAAG TGTACCCTCT GATCAGCCGG 
AGACGGTTCC AGCAAATGGA TGTACTTGAG GGCCTGAATG TCCTGGTGAC AATATCTGGC 
AAAAAGGATA AGTTACGCGT CTACTATTTA TCCTGGTTAA GAAATAAAAT ACTTCATAAT 
GATCCGGAAG TTGAGAAGAA GCAGGGGTGG ACCACCGTGG GCGACCTGGA AGGGTGCGTG 
CACTATAAAG TCGTAAAATA TGAAAGAATC AAGTTTCTGG TAATCGCTTT GAAGAGTTCT 
GTGGAAGTCT ATGCATGGGC ACCGAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT 
GGAGAACTGG TACATAAGCC ATTACTGGTG GATCTCACTG TCGAGGAAGG CCAGAGGTTG 
AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC CGGCTCAGTC 
TATGACATTT ATCTACCAAC ACACGTAAGA AAGAACCCAC ACTCTATGAT TCAGTGTACC 
ATCAAACCCC ATGCAATCAT TATCCTCCCC AACACGGATG GAATGGAGCT TCTGGTGTGC 
TACGAAGATG AAGGGGTTTA TGTGAACACG TACGGAAGAA TCACCAAGGA CGTGGTTCTG 
CAGTGGGGAG AGATGCCAAC ATCTGTAGCA TACATCCGGT CGAATCAGAC AATGGGCTGG 
GGAGAGAAGG CAATAGAGAT ACGCTCTGTG GAAACTGGTC ATTTGGACGG TGTGTTTATG 
CACAAAAGGG CTCAGAGACT AAAGTTCCTG TGTGAACGTA ATGACAAGGT GTTCTTTGCT 
TCCGTCCGCT CTGGCGGCAG CAGCCAGGTC TACTTCATGA CCTTAGGCAG GACTTCCCTT 
CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451835.1
CDS	209..3700
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017596346.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596346.1

ORF Insert Sequence:

ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG 
CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG 
TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG 
CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG 
ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC 
GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC 
ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG 
AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC 
CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA 
AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG 
CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG 
CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA 
CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG 
AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA 
CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA 
GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG 
ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA 
CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT 
CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTTCC TGTGAGAACG 
ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA 
AATAGCCAAG CAGGACAGAG AAATTCCACC AGTATTGAGC CCCGGCTTCT ATGGGAGAGA 
GTGGAAAAGC TGGTCCCCAG GCCAGGCAGT GGCAGCTCTT CAGGGTCCAG CAACTCAGGA 
TCCCAGCCTG GCTCCCATCC TGGGTCTCAG AGTGGCTCTG GTGAACGCTT CAGAGTGAGA 
TCATCATCCA AGTCTGAAGG TTCTCCATCT CAGCGCCTTG AAAATGCAGC CAAAAAACCT 
GAAGATAAGA AAGAAGTATT TCGACCTCTC AAACCTGCTG GAGAAGTGGA CTTGACTGCG 
TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTGCGGCCAC CTCACAAAGT GACAGACTAT 
TCCTCCTCCA GTGAGGAGTC TGGGACCACG GATGAGGAGG AGGAGGATGT GGAACAGGAG 
GGGGCTGATG ATTCCACCTC GGGGCCAGAG GACACCAGAG CAGCGTCGTC TCTGAATCTG 
AGCAACGGTG AGACAGAATC TGTGAAAACA ATGATTGTTC ATGATGACGT AGAAAGTGAA 
CCAGCCATGA CCCCATCCAA GGAGGGCACC CTAATCGTCC GCCAGACTCA GTCCGCTAGT 
AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGGTTA 
CTACAGATCT CTCCATCTAG TGGAACAACA GTTACTTCCG TTGTGGGATT TTCCTGTGAT 
GGGCTGAGAC CAGAAGCCAT AAGGCAAGAT CCTACTCGGA AAGGCTCAGT GGTCAATGTG 
AACCCCACGA ACACTAGGCC ACAGAGTGAT ACCCCCGAGA TTCGTAAATA CAAGAAGAGA 
TTTAACTCAG AGATCCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT TGGCACAGAG 
AGTGGCCTGA TGCTCTTGGA CAGAAGTGGC CAAGGGAAAG TGTACCCTCT GATCAGCCGG 
AGACGGTTCC AGCAAATGGA TGTACTTGAG GGCCTGAATG TCCTGGTGAC AATATCTGGC 
AAAAAGGATA AGTTACGCGT CTACTATTTA TCCTGGTTAA GAAATAAAAT ACTTCATAAT 
GATCCGGAAG TTGAGAAGAA GCAGGGGTGG ACCACCGTGG GCGACCTGGA AGGGTGCGTG 
CACTATAAAG TCGTAAAATA TGAAAGAATC AAGTTTCTGG TAATCGCTTT GAAGAGTTCT 
GTGGAAGTCT ATGCATGGGC ACCGAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT 
GGAGAACTGG TACATAAGCC ATTACTGGTG GATCTCACTG TCGAGGAAGG CCAGAGGTTG 
AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC CGGCTCAGTC 
TATGACATTT ATCTACCAAC ACACGTAAGA AAGAACCCAC ACTCTATGAT TCAGTGTACC 
ATCAAACCCC ATGCAATCAT TATCCTCCCC AACACGGATG GAATGGAGCT TCTGGTGTGC 
TACGAAGATG AAGGGGTTTA TGTGAACACG TACGGAAGAA TCACCAAGGA CGTGGTTCTG 
CAGTGGGGAG AGATGCCAAC ATCTGTAGCA TACATCCGGT CGAATCAGAC AATGGGCTGG 
GGAGAGAAGG CAATAGAGAT ACGCTCTGTG GAAACTGGTC ATTTGGACGG TGTGTTTATG 
CACAAAAGGG CTCAGAGACT AAAGTTCCTG TGTGAACGTA ATGACAAGGT GTTCTTTGCT 
TCCGTCCGCT CTGGCGGCAG CAGCCAGGTC TACTTCATGA CCTTAGGCAG GACTTCCCTT 
CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map4k4 ( NM_001106904.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100374.1 Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), mRNA.

Price & Availability↑

CloneID	ORa42317
Clone ID Related Accession (Same CDS sequence)	NM_001106904.1
Accession Version	NM_001106904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase kinase kinase kinase 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473965.2. On or before Oct 4, 2007 this sequence version replaced XM_001058712.1, XM_001058590.1, XM_001058649.1, XM_001058485.1, XM_001058541.1, XM_217381.4, XM_001058422.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGATGTCA CCGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
TATTCTCATC ATAGAAATAT TGCAACATAC TATGGTGCTT TCATTAAGAA GAGCCCTCCA 
GGACACGATG ACCAGCTCTG GCTCGTTATG GAGTTTTGCG GGGCTGGTTC CATCACAGAC 
CTTGTGAAGA ACACCAAAGG GAACACGCTC AAAGAAGACT GGATTGCTTA CATCTCCCGG 
GAAATCCTCA GGGGGTTGGC CCATCTCCAC ATTCACCATG TCATTCACCG AGACATCAAG 
GGGCAGAACG TGCTGCTGAC TGAGAACGCG GAGGTGAAAC TTGTTGATTT TGGTGTAAGC 
GCTCAGCTGG ACAGGACGGT TGGACGGAGA AATACATTCA TAGGCACACC ATACTGGATG 
GCTCCGGAGG TCATCGCCTG CGACGAGAAT CCAGATGCCA CTTACGACTA CAGAAGTGAC 
CTCTGGTCTT GTGGCATCAC GGCCATCGAG ATGGCAGAAG GAGCCCCCCC TCTCTGTGAC 
ATGCATCCAA TGAGAGCGCT GTTTCTCATC CCCAGAAACC CTCCTCCCAG GCTGAAGTCA 
AAAAAATGGT CAAAGAAATT TTTCAGCTTC ATAGAAGGCT GCCTGGTGAA GAATTACATG 
CAGCGGCCCT CTACAGAGCA GCTTTTAAAA CACCCTTTCA TAAGGGATCA GCCCAATGAA 
AGGCAGGTTC GAATCCAGCT TAAGGATCAC ATAGACCGGA CCAGGAAGAA GAGAGGCGAG 
AAAGATGAGA CGGAGTACGA GTACAGTGGC AGTGAGGAGG AGGAGGAGGA AGTGCCTGAG 
CAGGAGGGAG AGCCAAGTTC CATCGTCAAT GTGCCTGGAG AGTCGACTCT GCGACGTGAT 
TTCCTGAGGC TGCAGCAAGA GAACAAGGAA CGGTCTGAGG CTCTGCGGAG ACAGCAGCTT 
CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTATAAGA GGCAGCTGCT GGCTGAGAGG 
CAGAAGCGGA TTGAACAGCA GAAAGAACAG AGGAGGCGGC TGGAAGAGCA ACAAAGGAGA 
GAGCGTGAAG CCAGGAGGCA GCAGGAACGT GAGCAGCGGC GGCGAGAACA GGAGGAGAAG 
AGGCGTCTGG AGGAACTGGA AAGGCGGCGT AAGGAAGAGG AAGAGAGGAG ACGGGCAGAA 
GACGAGAAGA GGCGAGTGGA GCGGGAGCAG GAGTACATCA GGCGGCAGCT AGAGGAAGAG 
CAGCGGCACC TGGAGATCCT GCAGCAGCAG CTGCTCCAGG AGCAGGCTAT GTTACTGCAC 
GACCACAGGA GGCCACACGC ACAACAGCCG CCACCCCCGC AGCAGCAGGA CAGGAGCAAA 
CCCAGCTATC ACGCTCCAGA GCCCAAGCCT CACTACGACC CTGCTGACAG AGCTCGGGAG 
GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT 
TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT 
CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG 
CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG 
AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG 
CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA 
TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC 
AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGT AAGGATTGAC 
TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG 
ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG 
GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT 
CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA 
GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGACTCAG 
TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
CCCAGGTTAC TACAGATCTC TCCATCTAGT GGAACAACAG TTACTTCCGT TGTGGGATTT 
TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG 
GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC 
AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT 
GGCACAGAGA GTGGCCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT GTACCCTCTG 
ATCAGCCGGA GACGGTTCCA GCAAATGGAT GTACTTGAGG GCCTGAATGT CCTGGTGACA 
ATATCTGGCA AAAAGGATAA GTTACGCGTC TACTATTTAT CCTGGTTAAG AAATAAAATA 
CTTCATAATG ATCCGGAAGT TGAGAAGAAG CAGGGGTGGA CCACCGTGGG CGACCTGGAA 
GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATCGCTTTG 
AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT 
AAGTCATTTG GAGAACTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT CGAGGAAGGC 
CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCC 
GGCTCAGTCT ATGACATTTA TCTACCAACA CACATTCAGT GTACCATCAA ACCCCATGCA 
ATCATTATCC TCCCCAACAC GGATGGAATG GAGCTTCTGG TGTGCTACGA AGATGAAGGG 
GTTTATGTGA ACACGTACGG AAGAATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG 
CCAACATCTG TAGCATACAT CCGGTCGAAT CAGACAATGG GCTGGGGAGA GAAGGCAATA 
GAGATACGCT CTGTGGAAAC TGGTCATTTG GACGGTGTGT TTATGCACAA AAGGGCTCAG 
AGACTAAAGT TCCTGTGTGA ACGTAATGAC AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC 
GGCAGCAGCC AGGTCTACTT CATGACCTTA GGCAGGACTT CCCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100374.1
CDS	111..3647
Translation	MDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAVRIDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	NM_001106904.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106904.1

ORF Insert Sequence:

ATGGATGTCA CCGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
TATTCTCATC ATAGAAATAT TGCAACATAC TATGGTGCTT TCATTAAGAA GAGCCCTCCA 
GGACACGATG ACCAGCTCTG GCTCGTTATG GAGTTTTGCG GGGCTGGTTC CATCACAGAC 
CTTGTGAAGA ACACCAAAGG GAACACGCTC AAAGAAGACT GGATTGCTTA CATCTCCCGG 
GAAATCCTCA GGGGGTTGGC CCATCTCCAC ATTCACCATG TCATTCACCG AGACATCAAG 
GGGCAGAACG TGCTGCTGAC TGAGAACGCG GAGGTGAAAC TTGTTGATTT TGGTGTAAGC 
GCTCAGCTGG ACAGGACGGT TGGACGGAGA AATACATTCA TAGGCACACC ATACTGGATG 
GCTCCGGAGG TCATCGCCTG CGACGAGAAT CCAGATGCCA CTTACGACTA CAGAAGTGAC 
CTCTGGTCTT GTGGCATCAC GGCCATCGAG ATGGCAGAAG GAGCCCCCCC TCTCTGTGAC 
ATGCATCCAA TGAGAGCGCT GTTTCTCATC CCCAGAAACC CTCCTCCCAG GCTGAAGTCA 
AAAAAATGGT CAAAGAAATT TTTCAGCTTC ATAGAAGGCT GCCTGGTGAA GAATTACATG 
CAGCGGCCCT CTACAGAGCA GCTTTTAAAA CACCCTTTCA TAAGGGATCA GCCCAATGAA 
AGGCAGGTTC GAATCCAGCT TAAGGATCAC ATAGACCGGA CCAGGAAGAA GAGAGGCGAG 
AAAGATGAGA CGGAGTACGA GTACAGTGGC AGTGAGGAGG AGGAGGAGGA AGTGCCTGAG 
CAGGAGGGAG AGCCAAGTTC CATCGTCAAT GTGCCTGGAG AGTCGACTCT GCGACGTGAT 
TTCCTGAGGC TGCAGCAAGA GAACAAGGAA CGGTCTGAGG CTCTGCGGAG ACAGCAGCTT 
CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTATAAGA GGCAGCTGCT GGCTGAGAGG 
CAGAAGCGGA TTGAACAGCA GAAAGAACAG AGGAGGCGGC TGGAAGAGCA ACAAAGGAGA 
GAGCGTGAAG CCAGGAGGCA GCAGGAACGT GAGCAGCGGC GGCGAGAACA GGAGGAGAAG 
AGGCGTCTGG AGGAACTGGA AAGGCGGCGT AAGGAAGAGG AAGAGAGGAG ACGGGCAGAA 
GACGAGAAGA GGCGAGTGGA GCGGGAGCAG GAGTACATCA GGCGGCAGCT AGAGGAAGAG 
CAGCGGCACC TGGAGATCCT GCAGCAGCAG CTGCTCCAGG AGCAGGCTAT GTTACTGCAC 
GACCACAGGA GGCCACACGC ACAACAGCCG CCACCCCCGC AGCAGCAGGA CAGGAGCAAA 
CCCAGCTATC ACGCTCCAGA GCCCAAGCCT CACTACGACC CTGCTGACAG AGCTCGGGAG 
GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT 
TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT 
CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG 
CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG 
AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG 
CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA 
TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC 
AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGT AAGGATTGAC 
TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG 
ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG 
GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT 
CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA 
GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGACTCAG 
TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
CCCAGGTTAC TACAGATCTC TCCATCTAGT GGAACAACAG TTACTTCCGT TGTGGGATTT 
TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG 
GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC 
AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT 
GGCACAGAGA GTGGCCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT GTACCCTCTG 
ATCAGCCGGA GACGGTTCCA GCAAATGGAT GTACTTGAGG GCCTGAATGT CCTGGTGACA 
ATATCTGGCA AAAAGGATAA GTTACGCGTC TACTATTTAT CCTGGTTAAG AAATAAAATA 
CTTCATAATG ATCCGGAAGT TGAGAAGAAG CAGGGGTGGA CCACCGTGGG CGACCTGGAA 
GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATCGCTTTG 
AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT 
AAGTCATTTG GAGAACTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT CGAGGAAGGC 
CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCC 
GGCTCAGTCT ATGACATTTA TCTACCAACA CACATTCAGT GTACCATCAA ACCCCATGCA 
ATCATTATCC TCCCCAACAC GGATGGAATG GAGCTTCTGG TGTGCTACGA AGATGAAGGG 
GTTTATGTGA ACACGTACGG AAGAATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG 
CCAACATCTG TAGCATACAT CCGGTCGAAT CAGACAATGG GCTGGGGAGA GAAGGCAATA 
GAGATACGCT CTGTGGAAAC TGGTCATTTG GACGGTGTGT TTATGCACAA AAGGGCTCAG 
AGACTAAAGT TCCTGTGTGA ACGTAATGAC AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC 
GGCAGCAGCC AGGTCTACTT CATGACCTTA GGCAGGACTT CCCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Nck-interacting kinase NIK increases Arp2/3 complex activity by phosphorylating the Arp2 subunit.
The Journal of cell biology208(2)161-70(2015 Jan)
LeClaire LL,Rana M,Baumgartner M,Barber DL

Microtubules regulate focal adhesion dynamics through MAP4K4.
Developmental cell31(5)572-85(2014 Dec)
Yue J,Xie M,Gou X,Lee P,Schneider MD,Wu X

Identification of Map4k4 as a novel suppressor of skeletal muscle differentiation.
Molecular and cellular biology33(4)678-87(2013 Feb)
Wang M,Amano SU,Flach RJ,Chawla A,Aouadi M,Czech MP

A Novel Interaction between Pyk2 and MAP4K4 Is Integrated with Glioma Cell Migration.
Journal of signal transduction2013956580(2013)
Loftus JC,Yang Z,Kloss J,Dhruv H,Tran NL,Riggs DL

Rap2A links intestinal cell polarity to brush border formation.
Nature cell biology14(8)793-801(2012 Aug)
Gloerich M,ten Klooster JP,Vliem MJ,Koorman T,Zwartkruis FJ,Clevers H,Bos JL

REQUEST A QUOTE

EMAIL

[email protected]

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

Map4k4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: