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NLE1 cDNA ORF clone, Canis lupus familiaris(dog)

Summary

Gene Symbol NLE1
Entrez Gene ID 491142
Full Name notchless homolog 1
General protein information
Preferred Names

notchless homolog 1

Names

notchless protein homolog 1
Gene Type protein-coding
Organism Canis lupus familiaris(dog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_005624801.2 XP_005624858.1 notchless protein homolog 1 isoform X2
XM_014116748.1 XP_013972223.1 notchless protein homolog 1 isoform X1
XM_005624801.3 XP_005624858.1 notchless protein homolog 1 isoform X1
XM_014116749.1 XP_013972224.1 notchless protein homolog 1 isoform X3
XM_022423304.1 XP_022279012.1 notchless protein homolog 1 isoform X2

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) NLE1 XP_001174396.2
NLE1 XP_001113853.1
Mus musculus (house mouse) Nle1 NP_663406.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP002221 XP_307962.4
Schizosaccharomyces pombe (fission yeast) SPCC18.05c NP_588384.1
Neurospora crassa NCU00794 XP_964950.1
Rattus norvegicus (Norway rat) Nle1 NP_001121006.1
Caenorhabditis elegans (roundworm) W07E6.2 NP_493745.1
Kluyveromyces lactis KLLA0C08547g XP_452581.1
Eremothecium gossypii AGOS_AER263C NP_985120.1
Homo sapiens (human) NLE1 NP_060566.2
Canis lupus familiaris (dog) NLE1 XP_005624858.1
Danio rerio (zebrafish) nle1 NP_001018418.1
Drosophila melanogaster (fruit fly) Nle NP_477294.2
Gallus gallus (chicken) NLE1 XP_415857.3
Saccharomyces cerevisiae (baker's yeast) RSA4 NP_009997.2
Oryza sativa (rice) Os11g0594200 NP_001068204.1
Bos taurus (cattle) NLE1 NP_001014887.1
Magnaporthe oryzae (rice blast fungus) MGG_08708 XP_003719269.1
Arabidopsis thaliana (thale cress) AT5G52820 NP_200094.1
Oryza sativa (rice) Os10g0104500 NP_001064020.1
Xenopus tropicalis (tropical clawed frog) nle1 NP_001072540.1

Gene Ontology

The following NLE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe12131 XM_005624801.3
Latest version!		 Canis lupus familiaris notchless homolog 1 (NLE1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00
OCe12132 XM_022423304.1
Latest version!		 Canis lupus familiaris notchless homolog 1 (NLE1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $174.30
$249.00
OCe38470 XM_014116749.1
Latest version!		 Canis lupus familiaris notchless homolog 1 (Drosophila) (NLE1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $258.30
$369.00
OCe38469 XM_014116748.1
Latest version!		 Canis lupus familiaris notchless homolog 1 (Drosophila) (NLE1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $342.30
$489.00
View Old Accession Versions >>
OCe12131 XM_005624801.2 Canis lupus familiaris notchless homolog 1 (Drosophila) (NLE1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OCe12131
Clone ID Related Accession (Same CDS sequence) XM_005624801.2 , XM_005624801.3
Accession Version XM_005624801.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product notchless protein homolog 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624801.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGGGG TATCCCAGAC AGTGGTGGAA AAGAATAAAG TTTCAGCTGC CCACAGCCCA 
GTGGGGAGTG CTCCTGCCTG TCCTTTGATG GAGGAGTTGC CCTTTGCCAT TGCCCTCCAG 
GAGGATCCCT TGCCACTGGC TTTCTATGTC CACGATGCTG AGATCACTTC ATCACTGGGG 
AAGACGCTGG AGTCACAGGC AGTGGAGACA GAGAAGGTCC TTGACATCAT CTACCAGCCC 
CAGGCCATCT TCAGGGTCCG TGCTGTGACC CGCTGCACCA GCTCTTTGGA AGGTCACAGT 
GAGGCAGTCA TTTCTGTGGC CTTCAGCCCC ACAGGAAAGT ACCTGGCCAG TGGCTCCGGA 
GACACCACTG TGCGCTTCTG GGATCTCAGC ACCGAGACAC CGCATTTCAC ATGCCAGGGA 
CACAGACACT GGGTGCTTAG CATATCCTGG TCCCCAGACG GCAAAACACT GGCCTCAGGC 
TGCAAGAATG GCCAGATTCT CCTGTGGGAC CCAAGCACAG GGAAGCAGGT GGGCAGGGTC 
CTCGCCGGCC ATAGCAAGTG GATCACAGGC CTGAGTTGGG AGCCCCTCCA TGCGAACCCA 
GAATGCCGCT ACATGGCCAG CAGCTCCAAG GATGGCAGCG TGCGGGTCTG GGATACAACT 
GCAGGCCGCT GTGAGCGCAT CCTCACCGGG CACACGCAGT CAGTCACCTG TCTCCGGTGG 
GGAGGGGACG GGCTTCTCTA CTCTGCCTCG CAGGACCGTA CCATCAAAGT CTGGAGGGCT 
CATGATGGTG TGCTGTGCCG GACTCTGCAA GGCCATGGCC ACTGGGTGAA TACCATGGCA 
CTCAGCACTG ACTACGCCCT GCGCACAGGT GCCTTTGAGC CAGCTGAGGC CTCGATCAAT 
GCCCAAGACC TCCAAGGATC CTTGCAGGAG TTGAAGGACA GGGCTCTGAG GCGATACAAC 
CTCGTGCGGG GCCAGGGCCC AGAGAGGCTG GTGTCTGGCT CAGATGACTT CACCTTATTC 
ATGTGGTCCC CAGCAGAAGA CAAAAAGCCC CTTGCACGGA TGACGGGACA CCAGGCCCTC 
ATCAACCAGG TGCTCTTCTC CCCTGACTCC CGCATTATTG CCAGCGCTTC CTTCGACAAA 
TCCATCAAAC TGTGGGACGG CAGGACGGGC AAGTACCTGG CTTCCCTACG TGGCCACGTG 
GCTGCTGTGT ATCAGATCGC CTGGTCAGCC GATAGCCGGC TCCTGGTCAG TGGCAGCAGC 
GACAGCACGC TGAAGGTGTG GGATGTGAAG GCACAGAAGC TGGCAGCAGA TCTGCCAGGC 
CATGCAGATG AGGTATATGC TGTTGACTGG AGTCCAGATG GCCAGAGAGT GGCAAGTGGC 
GGGAAGGACA AATGCCTCCG GATATGGAGG AGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005624858.1
CDS590..2005
Translation

Target ORF information:

RefSeq Version XM_005624801.2
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris notchless homolog 1 (Drosophila) (NLE1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005624801.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGGGG TATCCCAGAC AGTGGTGGAA AAGAATAAAG TTTCAGCTGC CCACAGCCCA 
GTGGGGAGTG CTCCTGCCTG TCCTTTGATG GAGGAGTTGC CCTTTGCCAT TGCCCTCCAG 
GAGGATCCCT TGCCACTGGC TTTCTATGTC CACGATGCTG AGATCACTTC ATCACTGGGG 
AAGACGCTGG AGTCACAGGC AGTGGAGACA GAGAAGGTCC TTGACATCAT CTACCAGCCC 
CAGGCCATCT TCAGGGTCCG TGCTGTGACC CGCTGCACCA GCTCTTTGGA AGGTCACAGT 
GAGGCAGTCA TTTCTGTGGC CTTCAGCCCC ACAGGAAAGT ACCTGGCCAG TGGCTCCGGA 
GACACCACTG TGCGCTTCTG GGATCTCAGC ACCGAGACAC CGCATTTCAC ATGCCAGGGA 
CACAGACACT GGGTGCTTAG CATATCCTGG TCCCCAGACG GCAAAACACT GGCCTCAGGC 
TGCAAGAATG GCCAGATTCT CCTGTGGGAC CCAAGCACAG GGAAGCAGGT GGGCAGGGTC 
CTCGCCGGCC ATAGCAAGTG GATCACAGGC CTGAGTTGGG AGCCCCTCCA TGCGAACCCA 
GAATGCCGCT ACATGGCCAG CAGCTCCAAG GATGGCAGCG TGCGGGTCTG GGATACAACT 
GCAGGCCGCT GTGAGCGCAT CCTCACCGGG CACACGCAGT CAGTCACCTG TCTCCGGTGG 
GGAGGGGACG GGCTTCTCTA CTCTGCCTCG CAGGACCGTA CCATCAAAGT CTGGAGGGCT 
CATGATGGTG TGCTGTGCCG GACTCTGCAA GGCCATGGCC ACTGGGTGAA TACCATGGCA 
CTCAGCACTG ACTACGCCCT GCGCACAGGT GCCTTTGAGC CAGCTGAGGC CTCGATCAAT 
GCCCAAGACC TCCAAGGATC CTTGCAGGAG TTGAAGGACA GGGCTCTGAG GCGATACAAC 
CTCGTGCGGG GCCAGGGCCC AGAGAGGCTG GTGTCTGGCT CAGATGACTT CACCTTATTC 
ATGTGGTCCC CAGCAGAAGA CAAAAAGCCC CTTGCACGGA TGACGGGACA CCAGGCCCTC 
ATCAACCAGG TGCTCTTCTC CCCTGACTCC CGCATTATTG CCAGCGCTTC CTTCGACAAA 
TCCATCAAAC TGTGGGACGG CAGGACGGGC AAGTACCTGG CTTCCCTACG TGGCCACGTG 
GCTGCTGTGT ATCAGATCGC CTGGTCAGCC GATAGCCGGC TCCTGGTCAG TGGCAGCAGC 
GACAGCACGC TGAAGGTGTG GGATGTGAAG GCACAGAAGC TGGCAGCAGA TCTGCCAGGC 
CATGCAGATG AGGTATATGC TGTTGACTGG AGTCCAGATG GCCAGAGAGT GGCAAGTGGC 
GGGAAGGACA AATGCCTCCG GATATGGAGG AGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe12131
Clone ID Related Accession (Same CDS sequence) XM_005624801.2 , XM_005624801.3
Accession Version XM_005624801.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product notchless protein homolog 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624801.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGGGG TATCCCAGAC AGTGGTGGAA AAGAATAAAG TTTCAGCTGC CCACAGCCCA 
GTGGGGAGTG CTCCTGCCTG TCCTTTGATG GAGGAGTTGC CCTTTGCCAT TGCCCTCCAG 
GAGGATCCCT TGCCACTGGC TTTCTATGTC CACGATGCTG AGATCACTTC ATCACTGGGG 
AAGACGCTGG AGTCACAGGC AGTGGAGACA GAGAAGGTCC TTGACATCAT CTACCAGCCC 
CAGGCCATCT TCAGGGTCCG TGCTGTGACC CGCTGCACCA GCTCTTTGGA AGGTCACAGT 
GAGGCAGTCA TTTCTGTGGC CTTCAGCCCC ACAGGAAAGT ACCTGGCCAG TGGCTCCGGA 
GACACCACTG TGCGCTTCTG GGATCTCAGC ACCGAGACAC CGCATTTCAC ATGCCAGGGA 
CACAGACACT GGGTGCTTAG CATATCCTGG TCCCCAGACG GCAAAACACT GGCCTCAGGC 
TGCAAGAATG GCCAGATTCT CCTGTGGGAC CCAAGCACAG GGAAGCAGGT GGGCAGGGTC 
CTCGCCGGCC ATAGCAAGTG GATCACAGGC CTGAGTTGGG AGCCCCTCCA TGCGAACCCA 
GAATGCCGCT ACATGGCCAG CAGCTCCAAG GATGGCAGCG TGCGGGTCTG GGATACAACT 
GCAGGCCGCT GTGAGCGCAT CCTCACCGGG CACACGCAGT CAGTCACCTG TCTCCGGTGG 
GGAGGGGACG GGCTTCTCTA CTCTGCCTCG CAGGACCGTA CCATCAAAGT CTGGAGGGCT 
CATGATGGTG TGCTGTGCCG GACTCTGCAA GGCCATGGCC ACTGGGTGAA TACCATGGCA 
CTCAGCACTG ACTACGCCCT GCGCACAGGT GCCTTTGAGC CAGCTGAGGC CTCGATCAAT 
GCCCAAGACC TCCAAGGATC CTTGCAGGAG TTGAAGGACA GGGCTCTGAG GCGATACAAC 
CTCGTGCGGG GCCAGGGCCC AGAGAGGCTG GTGTCTGGCT CAGATGACTT CACCTTATTC 
ATGTGGTCCC CAGCAGAAGA CAAAAAGCCC CTTGCACGGA TGACGGGACA CCAGGCCCTC 
ATCAACCAGG TGCTCTTCTC CCCTGACTCC CGCATTATTG CCAGCGCTTC CTTCGACAAA 
TCCATCAAAC TGTGGGACGG CAGGACGGGC AAGTACCTGG CTTCCCTACG TGGCCACGTG 
GCTGCTGTGT ATCAGATCGC CTGGTCAGCC GATAGCCGGC TCCTGGTCAG TGGCAGCAGC 
GACAGCACGC TGAAGGTGTG GGATGTGAAG GCACAGAAGC TGGCAGCAGA TCTGCCAGGC 
CATGCAGATG AGGTATATGC TGTTGACTGG AGTCCAGATG GCCAGAGAGT GGCAAGTGGC 
GGGAAGGACA AATGCCTCCG GATATGGAGG AGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005624858.1
CDS588..2003
Translation

Target ORF information:

RefSeq Version XM_005624801.3
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris notchless homolog 1 (NLE1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005624801.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGGGG TATCCCAGAC AGTGGTGGAA AAGAATAAAG TTTCAGCTGC CCACAGCCCA 
GTGGGGAGTG CTCCTGCCTG TCCTTTGATG GAGGAGTTGC CCTTTGCCAT TGCCCTCCAG 
GAGGATCCCT TGCCACTGGC TTTCTATGTC CACGATGCTG AGATCACTTC ATCACTGGGG 
AAGACGCTGG AGTCACAGGC AGTGGAGACA GAGAAGGTCC TTGACATCAT CTACCAGCCC 
CAGGCCATCT TCAGGGTCCG TGCTGTGACC CGCTGCACCA GCTCTTTGGA AGGTCACAGT 
GAGGCAGTCA TTTCTGTGGC CTTCAGCCCC ACAGGAAAGT ACCTGGCCAG TGGCTCCGGA 
GACACCACTG TGCGCTTCTG GGATCTCAGC ACCGAGACAC CGCATTTCAC ATGCCAGGGA 
CACAGACACT GGGTGCTTAG CATATCCTGG TCCCCAGACG GCAAAACACT GGCCTCAGGC 
TGCAAGAATG GCCAGATTCT CCTGTGGGAC CCAAGCACAG GGAAGCAGGT GGGCAGGGTC 
CTCGCCGGCC ATAGCAAGTG GATCACAGGC CTGAGTTGGG AGCCCCTCCA TGCGAACCCA 
GAATGCCGCT ACATGGCCAG CAGCTCCAAG GATGGCAGCG TGCGGGTCTG GGATACAACT 
GCAGGCCGCT GTGAGCGCAT CCTCACCGGG CACACGCAGT CAGTCACCTG TCTCCGGTGG 
GGAGGGGACG GGCTTCTCTA CTCTGCCTCG CAGGACCGTA CCATCAAAGT CTGGAGGGCT 
CATGATGGTG TGCTGTGCCG GACTCTGCAA GGCCATGGCC ACTGGGTGAA TACCATGGCA 
CTCAGCACTG ACTACGCCCT GCGCACAGGT GCCTTTGAGC CAGCTGAGGC CTCGATCAAT 
GCCCAAGACC TCCAAGGATC CTTGCAGGAG TTGAAGGACA GGGCTCTGAG GCGATACAAC 
CTCGTGCGGG GCCAGGGCCC AGAGAGGCTG GTGTCTGGCT CAGATGACTT CACCTTATTC 
ATGTGGTCCC CAGCAGAAGA CAAAAAGCCC CTTGCACGGA TGACGGGACA CCAGGCCCTC 
ATCAACCAGG TGCTCTTCTC CCCTGACTCC CGCATTATTG CCAGCGCTTC CTTCGACAAA 
TCCATCAAAC TGTGGGACGG CAGGACGGGC AAGTACCTGG CTTCCCTACG TGGCCACGTG 
GCTGCTGTGT ATCAGATCGC CTGGTCAGCC GATAGCCGGC TCCTGGTCAG TGGCAGCAGC 
GACAGCACGC TGAAGGTGTG GGATGTGAAG GCACAGAAGC TGGCAGCAGA TCTGCCAGGC 
CATGCAGATG AGGTATATGC TGTTGACTGG AGTCCAGATG GCCAGAGAGT GGCAAGTGGC 
GGGAAGGACA AATGCCTCCG GATATGGAGG AGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe12132
Clone ID Related Accession (Same CDS sequence) XM_022423304.1
Accession Version XM_022423304.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 831bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product notchless protein homolog 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCCAGGA ACCCAGAATG CCGCTACATG GCCAGCAGCT CCAAGGATGG CAGCGTGCGG 
GTCTGGGATA CAACTGCAGG CCGCTGTGAG CGCATCCTCA CCGGGCACAC GCAGTCAGTC 
ACCTGTCTCC GGTGGGGAGG GGACGGGCTT CTCTACTCTG CCTCGCAGGA CCGTACCATC 
AAAGTCTGGA GGGCTCATGA TGGTGTGCTG TGCCGGACTC TGCAAGGCCA TGGCCACTGG 
GTGAATACCA TGGCACTCAG CACTGACTAC GCCCTGCGCA CAGGTGCCTT TGAGCCAGCT 
GAGGCCTCGA TCAATGCCCA AGACCTCCAA GGATCCTTGC AGGAGTTGAA GGACAGGGCT 
CTGAGGCGAT ACAACCTCGT GCGGGGCCAG GGCCCAGAGA GGCTGGTGTC TGGCTCAGAT 
GACTTCACCT TATTCATGTG GTCCCCAGCA GAAGACAAAA AGCCCCTTGC ACGGATGACG 
GGACACCAGG CCCTCATCAA CCAGGTGCTC TTCTCCCCTG ACTCCCGCAT TATTGCCAGC 
GCTTCCTTCG ACAAATCCAT CAAACTGTGG GACGGCAGGA CGGGCAAGTA CCTGGCTTCC 
CTACGTGGCC ACGTGGCTGC TGTGTATCAG ATCGCCTGGT CAGCCGATAG CCGGCTCCTG 
GTCAGTGGCA GCAGCGACAG CACGCTGAAG GTGTGGGATG TGAAGGCACA GAAGCTGGCA 
GCAGATCTGC CAGGCCATGC AGATGAGGTA TATGCTGTTG ACTGGAGTCC AGATGGCCAG 
AGAGTGGCAA GTGGCGGGAA GGACAAATGC CTCCGGATAT GGAGGAGATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_022279012.1
CDS114..944
Translation

Target ORF information:

RefSeq Version XM_022423304.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris notchless homolog 1 (NLE1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_022423304.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCCAGGA ACCCAGAATG CCGCTACATG GCCAGCAGCT CCAAGGATGG CAGCGTGCGG 
GTCTGGGATA CAACTGCAGG CCGCTGTGAG CGCATCCTCA CCGGGCACAC GCAGTCAGTC 
ACCTGTCTCC GGTGGGGAGG GGACGGGCTT CTCTACTCTG CCTCGCAGGA CCGTACCATC 
AAAGTCTGGA GGGCTCATGA TGGTGTGCTG TGCCGGACTC TGCAAGGCCA TGGCCACTGG 
GTGAATACCA TGGCACTCAG CACTGACTAC GCCCTGCGCA CAGGTGCCTT TGAGCCAGCT 
GAGGCCTCGA TCAATGCCCA AGACCTCCAA GGATCCTTGC AGGAGTTGAA GGACAGGGCT 
CTGAGGCGAT ACAACCTCGT GCGGGGCCAG GGCCCAGAGA GGCTGGTGTC TGGCTCAGAT 
GACTTCACCT TATTCATGTG GTCCCCAGCA GAAGACAAAA AGCCCCTTGC ACGGATGACG 
GGACACCAGG CCCTCATCAA CCAGGTGCTC TTCTCCCCTG ACTCCCGCAT TATTGCCAGC 
GCTTCCTTCG ACAAATCCAT CAAACTGTGG GACGGCAGGA CGGGCAAGTA CCTGGCTTCC 
CTACGTGGCC ACGTGGCTGC TGTGTATCAG ATCGCCTGGT CAGCCGATAG CCGGCTCCTG 
GTCAGTGGCA GCAGCGACAG CACGCTGAAG GTGTGGGATG TGAAGGCACA GAAGCTGGCA 
GCAGATCTGC CAGGCCATGC AGATGAGGTA TATGCTGTTG ACTGGAGTCC AGATGGCCAG 
AGAGTGGCAA GTGGCGGGAA GGACAAATGC CTCCGGATAT GGAGGAGATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe38470
Clone ID Related Accession (Same CDS sequence) XM_014116749.1
Accession Version XM_014116749.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1152bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product notchless protein homolog 1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGTGGGGG TATCCCAGAC AGTGGTGGAA AAGAATAAAG TTTCAGCTGC CCACAGCCCA 
GTGGGGAGTG CTCCTGCCTG TCCTTTGATG GAGGAGTTGC CCTTTGCCAT TGCCCTCCAG 
GAGGATCCCT TGCCACTGGC TTTCTATGTC CACGATGCTG AGATCACTTC ATCACTGGGG 
AAGACGCTGG AGTCACAGGC AGTGGAGACA GAGAAGGTCC TTGACATCAT CTACCAGCCC 
CAGGCCATCT TCAGGGTCCG TGCTGTGACC CGCTGCACCA GCTCTTTGGA AGGTCACAGT 
GAGGCAGTCA TTTCTGTGGC CTTCAGCCCC ACAGGAAAGT ACCTGGCCAG TGGCTCCGGA 
GACACCACTG TGCGCTTCTG GGATCTCAGC ACCGAGACAC CGCATTTCAC ATGCCAGGGA 
CACAGACACT GGGTGCTTAG CATATCCTGG TCCCCAGACG GCAAAACACT GGCCTCAGGC 
TGCAAGAATG GCCAGATTCT CCTGTGGGAC CCAAGCACAG GGAAGCAGGT GGGCAGGGTC 
CTCGCCGGCC ATAGCAAGTG GATCACAGGC CTGAGTTGGG AGCCCCTCCA TGCGAACCCA 
GAATGCCGCT ACATGGCCAG CAGCTCCAAG GATGGCAGCG TGCGGGTCTG GGATACAACT 
GCAGGCCGCT GTGAGCGCAT CCTCACCGGG CACACGCAGT CAGTCACCTG TCTCCGGTGG 
GGAGGGGACG GGCTTCTCTA CTCTGCCTCG CAGGACCGTA CCATCAAAGT CTGGAGGGCT 
CATGATGGTG TGCTGTGCCG GACTCTGCAA GGCCATGGCC ACTGGGTGAA TACCATGGCA 
CTCAGCACTG ACTACGCCCT GCGCACAGGT GCCTTTGAGC CAGCTGAGGC CTCGATCAAT 
GCCCAAGACC TCCAAGGATC CTTGCAGGAG TTGAAGGACA GGGCTCTGAG GCGATACAAC 
CTCGTGCGGG GCCAGGGCCC AGAGAGGCTG GTGTCTGGCT CAGATGACTT CACCTTATTC 
ATGTGGTCCC CAGCAGAAGA CAAAAAGCCC CTTGCACGGA TGACGGGACA CCAGGCCCTC 
ATCAACCAGG TACCTGGCTT CCCTACGTGG CCACGTGGCT GCTGTGTATC AGATCGCCTG 
GTCAGCCGAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_013972224.1
CDS591..1742
Translation

Target ORF information:

RefSeq Version XM_014116749.1
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris notchless homolog 1 (Drosophila) (NLE1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014116749.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGTGGGGG TATCCCAGAC AGTGGTGGAA AAGAATAAAG TTTCAGCTGC CCACAGCCCA 
GTGGGGAGTG CTCCTGCCTG TCCTTTGATG GAGGAGTTGC CCTTTGCCAT TGCCCTCCAG 
GAGGATCCCT TGCCACTGGC TTTCTATGTC CACGATGCTG AGATCACTTC ATCACTGGGG 
AAGACGCTGG AGTCACAGGC AGTGGAGACA GAGAAGGTCC TTGACATCAT CTACCAGCCC 
CAGGCCATCT TCAGGGTCCG TGCTGTGACC CGCTGCACCA GCTCTTTGGA AGGTCACAGT 
GAGGCAGTCA TTTCTGTGGC CTTCAGCCCC ACAGGAAAGT ACCTGGCCAG TGGCTCCGGA 
GACACCACTG TGCGCTTCTG GGATCTCAGC ACCGAGACAC CGCATTTCAC ATGCCAGGGA 
CACAGACACT GGGTGCTTAG CATATCCTGG TCCCCAGACG GCAAAACACT GGCCTCAGGC 
TGCAAGAATG GCCAGATTCT CCTGTGGGAC CCAAGCACAG GGAAGCAGGT GGGCAGGGTC 
CTCGCCGGCC ATAGCAAGTG GATCACAGGC CTGAGTTGGG AGCCCCTCCA TGCGAACCCA 
GAATGCCGCT ACATGGCCAG CAGCTCCAAG GATGGCAGCG TGCGGGTCTG GGATACAACT 
GCAGGCCGCT GTGAGCGCAT CCTCACCGGG CACACGCAGT CAGTCACCTG TCTCCGGTGG 
GGAGGGGACG GGCTTCTCTA CTCTGCCTCG CAGGACCGTA CCATCAAAGT CTGGAGGGCT 
CATGATGGTG TGCTGTGCCG GACTCTGCAA GGCCATGGCC ACTGGGTGAA TACCATGGCA 
CTCAGCACTG ACTACGCCCT GCGCACAGGT GCCTTTGAGC CAGCTGAGGC CTCGATCAAT 
GCCCAAGACC TCCAAGGATC CTTGCAGGAG TTGAAGGACA GGGCTCTGAG GCGATACAAC 
CTCGTGCGGG GCCAGGGCCC AGAGAGGCTG GTGTCTGGCT CAGATGACTT CACCTTATTC 
ATGTGGTCCC CAGCAGAAGA CAAAAAGCCC CTTGCACGGA TGACGGGACA CCAGGCCCTC 
ATCAACCAGG TACCTGGCTT CCCTACGTGG CCACGTGGCT GCTGTGTATC AGATCGCCTG 
GTCAGCCGAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe38469
Clone ID Related Accession (Same CDS sequence) XM_014116748.1
Accession Version XM_014116748.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1566bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product notchless protein homolog 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGTGGGGG TATCCCAGAC AGTGGTGGAA AAGAATAAAG TTTCAGCTGC CCACAGCCCA 
GTGGGGAGTG CTCCTGCCTG TCCTTTGATG GAGGAGTTGC CCTTTGCCAT TGCCCTCCAG 
GAGGATCCCT TGCCACTGGC TTTCTATGTC CACGATGCTG AGATCACTTC ATCACTGGGG 
AAGACGCTGG AGTCACAGGC AGTGGAGACA GAGAAGGTCC TTGACATCAT CTACCAGCCC 
CAGGCCATCT TCAGGGTCCG TGCTGTGACC CGCTGCACCA GCTCTTTGGA AGGTCACAGT 
GAGGCAGTCA TTTCTGTGGC CTTCAGCCCC ACAGGAAAGT ACCTGGCCAG TGGCTCCGGA 
GACACCACTG TGCGCTTCTG GGATCTCAGC ACCGAGACAC CGCATTTCAC ATGCCAGGGA 
CACAGACACT GGGTGCTTAG CATATCCTGG TCCCCAGACG GCAAAACACT GGCCTCAGGC 
TGCAAGAATG GCCAGATTCT CCTGTGGGAC CCAAGCACAG GGAAGCAGGT GGGCAGGGTC 
CTCGCCGGCC ATAGCAAGTG GATCACAGGC CTGAGTTGGG AGCCCCTCCA TGCGAACCCA 
GAATGCCGCT ACATGGCCAG CAGCTCCAAG GATGGCAGCG TGCGGGTCTG GGATACAACT 
GCAGGCCGCT GTGAGCGCAT CCTCACCGGG CACACGCAGT CAGTCACCTG TCTCCGGTGG 
GGAGGGGACG GGCTTCTCTA CTCTGCCTCG CAGGACCGTA CCATCAAAGT CTGGAGGGCT 
CATGATGGTG TGCTGTGCCG GACTCTGCAA GGCCATGGCC ACTGGGTGAA TACCATGGCA 
CTCAGCACTG ACTACGCCCT GCGCACAGGT GCCTTTGAGC CAGCTGAGGC CTCGATCAAT 
GCCCAAGACC TCCAAGGATC CTTGCAGGAG TTGAAGGACA GGGCTCTGAG GCGATACAAC 
CTCGTGCGGG GCCAGGGCCC AGAGAGGCTG GTGTCTGGCT CAGATGACTT CACCTTATTC 
ATGTGGTCCC CAGCAGAAGA CAAAAAGCCC CTTGCACGGA TGACGGGACA CCAGGCCCTC 
ATCAACCAGG TGCTCTTCTC CCCTGACTCC CGCATTATTG CCAGCGCTTC CTTCGACAAA 
TCCATCAAAC TGTGGGACGG CAGGACGGGC AAGTACCTGG CTTCCCTACG TGGCCACGTG 
GCTGCTGTGT ATCAGATCGC CTGGTCAGCC GATAGCCGGC TCCTGGTCAG TGGCAGCAGC 
GACAGCACGC TGAAGGTGTG GGATGTGAAG GCACAGAAGC TGGCAGCAGA TCTGCCAGGC 
CATGCAGATG AGGTATATGC TGTTGACTGG AGTCCAGATG GCCAGAGAGT GGCAAGTGGC 
GGGAAGGACA AATGCCTCCG GATAAACGCA GATGCCCTCT CTGCGCTCTG CTGCTTCCCA 
GCACCTGCCA CCCAATCTGT AATTACCCGG TATCTTCGAT TCCTCTTGGC TGTCCTCGTC 
ACTAACAGGG AGGCGTCTCG GGCAGGGAAG TGGTGGACCT GGCTTCTGCA GACCTGGAGG 
CCGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_013972223.1
CDS591..2156
Translation

Target ORF information:

RefSeq Version XM_014116748.1
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris notchless homolog 1 (Drosophila) (NLE1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014116748.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGTGGGGG TATCCCAGAC AGTGGTGGAA AAGAATAAAG TTTCAGCTGC CCACAGCCCA 
GTGGGGAGTG CTCCTGCCTG TCCTTTGATG GAGGAGTTGC CCTTTGCCAT TGCCCTCCAG 
GAGGATCCCT TGCCACTGGC TTTCTATGTC CACGATGCTG AGATCACTTC ATCACTGGGG 
AAGACGCTGG AGTCACAGGC AGTGGAGACA GAGAAGGTCC TTGACATCAT CTACCAGCCC 
CAGGCCATCT TCAGGGTCCG TGCTGTGACC CGCTGCACCA GCTCTTTGGA AGGTCACAGT 
GAGGCAGTCA TTTCTGTGGC CTTCAGCCCC ACAGGAAAGT ACCTGGCCAG TGGCTCCGGA 
GACACCACTG TGCGCTTCTG GGATCTCAGC ACCGAGACAC CGCATTTCAC ATGCCAGGGA 
CACAGACACT GGGTGCTTAG CATATCCTGG TCCCCAGACG GCAAAACACT GGCCTCAGGC 
TGCAAGAATG GCCAGATTCT CCTGTGGGAC CCAAGCACAG GGAAGCAGGT GGGCAGGGTC 
CTCGCCGGCC ATAGCAAGTG GATCACAGGC CTGAGTTGGG AGCCCCTCCA TGCGAACCCA 
GAATGCCGCT ACATGGCCAG CAGCTCCAAG GATGGCAGCG TGCGGGTCTG GGATACAACT 
GCAGGCCGCT GTGAGCGCAT CCTCACCGGG CACACGCAGT CAGTCACCTG TCTCCGGTGG 
GGAGGGGACG GGCTTCTCTA CTCTGCCTCG CAGGACCGTA CCATCAAAGT CTGGAGGGCT 
CATGATGGTG TGCTGTGCCG GACTCTGCAA GGCCATGGCC ACTGGGTGAA TACCATGGCA 
CTCAGCACTG ACTACGCCCT GCGCACAGGT GCCTTTGAGC CAGCTGAGGC CTCGATCAAT 
GCCCAAGACC TCCAAGGATC CTTGCAGGAG TTGAAGGACA GGGCTCTGAG GCGATACAAC 
CTCGTGCGGG GCCAGGGCCC AGAGAGGCTG GTGTCTGGCT CAGATGACTT CACCTTATTC 
ATGTGGTCCC CAGCAGAAGA CAAAAAGCCC CTTGCACGGA TGACGGGACA CCAGGCCCTC 
ATCAACCAGG TGCTCTTCTC CCCTGACTCC CGCATTATTG CCAGCGCTTC CTTCGACAAA 
TCCATCAAAC TGTGGGACGG CAGGACGGGC AAGTACCTGG CTTCCCTACG TGGCCACGTG 
GCTGCTGTGT ATCAGATCGC CTGGTCAGCC GATAGCCGGC TCCTGGTCAG TGGCAGCAGC 
GACAGCACGC TGAAGGTGTG GGATGTGAAG GCACAGAAGC TGGCAGCAGA TCTGCCAGGC 
CATGCAGATG AGGTATATGC TGTTGACTGG AGTCCAGATG GCCAGAGAGT GGCAAGTGGC 
GGGAAGGACA AATGCCTCCG GATAAACGCA GATGCCCTCT CTGCGCTCTG CTGCTTCCCA 
GCACCTGCCA CCCAATCTGT AATTACCCGG TATCTTCGAT TCCTCTTGGC TGTCCTCGTC 
ACTAACAGGG AGGCGTCTCG GGCAGGGAAG TGGTGGACCT GGCTTCTGCA GACCTGGAGG 
CCGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed