The following SHHA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHHA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID |
ODa02261 |
| Clone ID Related Accession (Same CDS sequence) |
NM_131063.3
|
| Accession Version |
NM_131063.3 Latest version! |
Documents for ORF clone product in default vector |
| Sequence Information |
ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP |
| Vector |
pcDNA3.1+/C-(K)DYK or customized vector |
 User Manual |
| Clone information |
Clone Map |
MSDS |
| Tag on pcDNA3.1+/C-(K)DYK |
C terminal DYKDDDDK tags |
| ORF Insert Method |
CloneEZ™ Seamless cloning technology |
| Insert Structure |
linear |
| Update Date |
2020-01-04 |
| Organism |
Danio rerio(zebrafish) |
| Product |
sonic hedgehog protein A precursor |
| Comment |
Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from L27585.1, CU041319.16 and CT668143.2. On Jun 11, 2014 this sequence version replaced NM_131063.2.
Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: L27585.1, BC162395.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
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1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
| ATGCGGCTTT TGACGAGAGT GCTGCTGGTG TCTCTTCTCA CTCTGTCCTT GGTGGTGTCC
GGACTGGCCT GCGGTCCTGG CAGAGGCTAC GGCAGAAGAA GACATCCGAA GAAGCTGACA
CCTCTCGCCT ACAAGCAGTT CATACCTAAT GTCGCGGAGA AGACCTTAGG GGCCAGCGGC
AGATACGAGG GCAAGATAAC GCGCAATTCG GAGAGATTTA AAGAACTTAC TCCAAATTAT
AATCCCGACA TTATCTTTAA GGATGAGGAG AACACGGGAG CGGACAGGCT CATGACACAG
AGATGCAAAG ACAAGCTGAA CTCGCTGGCC ATCTCTGTAA TGAACCACTG GCCAGGGGTT
AAGCTGCGTG TGACAGAGGG CTGGGATGAG GACGGTCACC ATTTTGAAGA ATCACTCCAC
TACGAGGGAA GAGCTGTTGA TATTACCACC TCTGACCGAG ACAAGAGCAA ATACGGGACA
CTGTCTCGCC TAGCTGTGGA GGCTGGATTT GACTGGGTCT ATTACGAGTC CAAAGCCCAC
ATTCATTGCT CTGTCAAAGC AGAAAATTCG GTTGCTGCGA AATCTGGGGG CTGTTTCCCA
GGTTCGGCTC TGGTCTCGCT CCAGGACGGA GGACAGAAGG CCGTGAAGGA CCTGAACCCC
GGAGACAAGG TGCTGGCGGC AGACAGCGCG GGAAACCTGG TGTTCAGCGA CTTCATCATG
TTCACAGACC GAGACTCCAC GACGCGACGT GTGTTTTACG TCATAGAAAC GCAAGAACCC
GTTGAAAAGA TCACCCTCAC CGCCGCTCAC CTCCTTTTTG TCCTCGACAA CTCAACGGAA
GATCTCCACA CCATGACCGC CGCGTATGCC AGCAGTGTCA GAGCCGGACA AAAGGTGATG
GTTGTTGATG ATAGCGGCCA GCTTAAATCT GTCATCGTGC AGCGGATATA CACGGAGGAG
CAGCGGGGCT CGTTCGCACC AGTGACTGCA CATGGGACCA TTGTGGTCGA CAGAATACTG
GCGTCCTGTT ACGCCGTAAT AGAGGACCAG GGGCTTGCGC ATTTGGCCTT CGCGCCCGCC
AGGCTGTATT ATTACGTGTC ATCATTCCTG TTCCCCCAAA ACTCCAGCAG TCGGTCCAAT
GCGACTTTAC AACAGGAGGG GGTCCACTGG TACTCCAGGC TCCTGTATCA AATGGGAACG
TGGCTTTTGG ACAGCAACAT GCTTCATCCT TTGGGGATGT CAGTAAACTC AAGCTGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq |
NP_571138.1 |
| CDS | 320..1576 |
| Translation | |
Target ORF information:
| RefSeq Version | NM_131063.3 |
| Organism | Danio rerio(zebrafish) |
| Definition | Danio rerio sonic hedgehog signaling molecule a (shha), mRNA. |
Target ORF information:
| Epitope | DYKDDDDK |
| Bacterial selection | AMPR |
| Mammalian selection | NeoR |
| Vector | pcDNA3.1+/C-(K)DYK |
|
 NM_131063.3 |
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
| ATGCGGCTTT TGACGAGAGT GCTGCTGGTG TCTCTTCTCA CTCTGTCCTT GGTGGTGTCC
GGACTGGCCT GCGGTCCTGG CAGAGGCTAC GGCAGAAGAA GACATCCGAA GAAGCTGACA
CCTCTCGCCT ACAAGCAGTT CATACCTAAT GTCGCGGAGA AGACCTTAGG GGCCAGCGGC
AGATACGAGG GCAAGATAAC GCGCAATTCG GAGAGATTTA AAGAACTTAC TCCAAATTAT
AATCCCGACA TTATCTTTAA GGATGAGGAG AACACGGGAG CGGACAGGCT CATGACACAG
AGATGCAAAG ACAAGCTGAA CTCGCTGGCC ATCTCTGTAA TGAACCACTG GCCAGGGGTT
AAGCTGCGTG TGACAGAGGG CTGGGATGAG GACGGTCACC ATTTTGAAGA ATCACTCCAC
TACGAGGGAA GAGCTGTTGA TATTACCACC TCTGACCGAG ACAAGAGCAA ATACGGGACA
CTGTCTCGCC TAGCTGTGGA GGCTGGATTT GACTGGGTCT ATTACGAGTC CAAAGCCCAC
ATTCATTGCT CTGTCAAAGC AGAAAATTCG GTTGCTGCGA AATCTGGGGG CTGTTTCCCA
GGTTCGGCTC TGGTCTCGCT CCAGGACGGA GGACAGAAGG CCGTGAAGGA CCTGAACCCC
GGAGACAAGG TGCTGGCGGC AGACAGCGCG GGAAACCTGG TGTTCAGCGA CTTCATCATG
TTCACAGACC GAGACTCCAC GACGCGACGT GTGTTTTACG TCATAGAAAC GCAAGAACCC
GTTGAAAAGA TCACCCTCAC CGCCGCTCAC CTCCTTTTTG TCCTCGACAA CTCAACGGAA
GATCTCCACA CCATGACCGC CGCGTATGCC AGCAGTGTCA GAGCCGGACA AAAGGTGATG
GTTGTTGATG ATAGCGGCCA GCTTAAATCT GTCATCGTGC AGCGGATATA CACGGAGGAG
CAGCGGGGCT CGTTCGCACC AGTGACTGCA CATGGGACCA TTGTGGTCGA CAGAATACTG
GCGTCCTGTT ACGCCGTAAT AGAGGACCAG GGGCTTGCGC ATTTGGCCTT CGCGCCCGCC
AGGCTGTATT ATTACGTGTC ATCATTCCTG TTCCCCCAAA ACTCCAGCAG TCGGTCCAAT
GCGACTTTAC AACAGGAGGG GGTCCACTGG TACTCCAGGC TCCTGTATCA AATGGGAACG
TGGCTTTTGG ACAGCAACAT GCTTCATCCT TTGGGGATGT CAGTAAACTC AAGCTGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID |
ODa39205 |
| Clone ID Related Accession (Same CDS sequence) |
XM_009303382.2
|
| Accession Version |
XM_009303382.2 Latest version! |
Documents for ORF clone product in default vector |
| Sequence Information |
ORF Nucleotide Sequence (Length: 918bp)
Protein sequence
SNP |
| Vector |
pcDNA3.1+/C-(K)DYK or customized vector |
User Manual |
| Clone information |
Clone Map |
MSDS |
| Tag on pcDNA3.1+/C-(K)DYK |
C terminal DYKDDDDK tags |
| ORF Insert Method |
CloneEZ™ Seamless cloning technology |
| Insert Structure |
linear |
| Update Date |
2016-06-23 |
| Organism |
Danio rerio(Zebrafish) |
| Product |
sonic hedgehog protein A isoform X1 |
| Comment |
MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process
On Jun 24, 2016 this sequence version replaced XM_009303382.1.
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Status :: Full annotation
Annotation Version :: Danio rerio Annotation Release 105
Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline
Annotation Software Version :: 7.1
Annotation Method :: Best-placed RefSeq; Gnomon
Features Annotated :: Gene; mRNA; CDS; ncRNA
##Genome-Annotation-Data-END##
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1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
| ATGAACCACT GGCCAGGGGT TAAGCTGCGT GTGACAGAGG GCTGGGATGA GGACGGTCAC
CATTTTGAAG AATCACTCCA CTACGAGGGA AGAGCTGTTG ATATTACCAC CTCTGACCGA
GACAAGAGCA AATACGGGAC ACTGTCTCGC CTAGCTGTGG AGGCTGGATT TGACTGGGTC
TATTACGAGT CCAAAGCCCA CATTCATTGC TCTGTCAAAG CAGAAAATTC GGTTGCTGCG
AAATCTGGGG GCTGTTTCCC AGGTTCGGCT CTGGTCTCGC TCCAGGACGG AGGACAGAAG
GCCGTGAAGG ACCTGAACCC CGGAGACAAG GTGCTGGCGG CAGACAGCGC GGGAAACCTG
GTGTTCAGCG ACTTCATCAT GTTCACAGAC CGAGACTCCA CGACGCGACG TGTGTTTTAC
GTCATAGAAA CGCAAGAACC CGTTGAAAAG ATCACCCTCA CCGCCGCTCA CCTCCTTTTT
GTCCTCGACA ACTCAACGGA AGATCTCCAC ACCATGACCG CCGCGTATGC CAGCAGTGTC
AGAGCCGGAC AAAAGGTGAT GGTTGTTGAT GATAGCGGCC AGCTTAAATC TGTCATCGTG
CAGCGGATAT ACACGGAGGA GCAGCGGGGC TCGTTCGCAC CAGTGACTGC ACATGGGACC
ATTGTGGTCG ACAGAATACT GGCGTCCTGT TACGCCGTAA TAGAGGACCA GGGGCTTGCG
CATTTGGCCT TCGCGCCCGC CAGGCTGTAT TATTACGTGT CATCATTCCT GTTCCCCCAA
AACTCCAGCA GTCGGTCCAA TGCGACTTTA CAACAGGAGG GGGTCCACTG GTACTCCAGG
CTCCTGTATC AAATGGGAAC GTGGCTTTTG GACAGCAACA TGCTTCATCC TTTGGGGATG
TCAGTAAACT CAAGCTGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq |
XP_009301657.1 |
| CDS | 183..1100 |
| Translation | |
Target ORF information:
| RefSeq Version | XM_009303382.2 |
| Organism | Danio rerio(Zebrafish) |
| Definition | Danio rerio sonic hedgehog a (shha), transcript variant X1, mRNA. |
Target ORF information:
| Epitope | DYKDDDDK |
| Bacterial selection | AMPR |
| Mammalian selection | NeoR |
| Vector | pcDNA3.1+/C-(K)DYK |
|
XM_009303382.2 |
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
| ATGAACCACT GGCCAGGGGT TAAGCTGCGT GTGACAGAGG GCTGGGATGA GGACGGTCAC
CATTTTGAAG AATCACTCCA CTACGAGGGA AGAGCTGTTG ATATTACCAC CTCTGACCGA
GACAAGAGCA AATACGGGAC ACTGTCTCGC CTAGCTGTGG AGGCTGGATT TGACTGGGTC
TATTACGAGT CCAAAGCCCA CATTCATTGC TCTGTCAAAG CAGAAAATTC GGTTGCTGCG
AAATCTGGGG GCTGTTTCCC AGGTTCGGCT CTGGTCTCGC TCCAGGACGG AGGACAGAAG
GCCGTGAAGG ACCTGAACCC CGGAGACAAG GTGCTGGCGG CAGACAGCGC GGGAAACCTG
GTGTTCAGCG ACTTCATCAT GTTCACAGAC CGAGACTCCA CGACGCGACG TGTGTTTTAC
GTCATAGAAA CGCAAGAACC CGTTGAAAAG ATCACCCTCA CCGCCGCTCA CCTCCTTTTT
GTCCTCGACA ACTCAACGGA AGATCTCCAC ACCATGACCG CCGCGTATGC CAGCAGTGTC
AGAGCCGGAC AAAAGGTGAT GGTTGTTGAT GATAGCGGCC AGCTTAAATC TGTCATCGTG
CAGCGGATAT ACACGGAGGA GCAGCGGGGC TCGTTCGCAC CAGTGACTGC ACATGGGACC
ATTGTGGTCG ACAGAATACT GGCGTCCTGT TACGCCGTAA TAGAGGACCA GGGGCTTGCG
CATTTGGCCT TCGCGCCCGC CAGGCTGTAT TATTACGTGT CATCATTCCT GTTCCCCCAA
AACTCCAGCA GTCGGTCCAA TGCGACTTTA CAACAGGAGG GGGTCCACTG GTACTCCAGG
CTCCTGTATC AAATGGGAAC GTGGCTTTTG GACAGCAACA TGCTTCATCC TTTGGGGATG
TCAGTAAACT CAAGCTGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
