tardbp cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tardbp
Entrez Gene ID	325052
Full Name	TAR DNA binding protein
Synonyms	wu:fb77f02,wu:fc52g10
General protein information	Preferred Names TAR DNA binding protein Names TAR DNA-binding protein 43
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_201476.1	NP_958884.1	TAR DNA-binding protein 43

Pathways from BioSystems

Homologies

Homo sapiens (human)	TARDBP	NP_031401.1
Bos taurus (cattle)	TARDBP	NP_001039950.2
	TARDBP	XP_001102660.1
Pan troglodytes (chimpanzee)	TARDBP	XP_514380.2
Danio rerio (zebrafish)	tardbp	NP_958884.1
Xenopus tropicalis (tropical clawed frog)	tardbp	NP_989054.2
Canis lupus familiaris (dog)	TARDBP	XP_005618063.1
Mus musculus (house mouse)	Tardbp	NP_663531.1
Gallus gallus (chicken)	TARDBP	NP_001026049.1

Gene Ontology
Bibliography

Related articles in PubMed

Dynamic RNA-protein interactions underlie the zebrafish maternal-to-zygotic transition.
Despic V, Dejung M, Gu M, Krishnan J, Zhang J, Herzel L, Straube K, Gerstein MB, Butter F, Neugebauer KM
Genome research27(7)1184-1194(2017 Jul)

Augmented quantal release of acetylcholine at the vertebrate neuromuscular junction following tdp-43 depletion.
Dzieciolowska S, Drapeau P, Armstrong GAB
PloS one12(5)e0177005(2017)

Neurotrophic effects of progranulin in vivo in reversing motor neuron defects caused by over or under expression of TDP-43 or FUS.
Chitramuthu BP, Kay DG, Bateman A, Bennett HP
PloS one12(3)e0174784(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate a method for site-directed single nucleotide editing in two disease-related genes, DNA binding protein tardbp and RNA binding protein fus.
Title: Homology Directed Knockin of Point Mutations in the Zebrafish tardbp and fus Genes in ALS Using the CRISPR/Cas9 System.

Loss of ALS-associated TDP-43 in zebrafish causes muscle degeneration, vascular dysfunction, and reduced motor neuron axon outgrowth.
Title: Loss of ALS-associated TDP-43 in zebrafish causes muscle degeneration, vascular dysfunction, and reduced motor neuron axon outgrowth.

TARDBP and FUS act in a pathogenic pathway that is independent of SOD1.
Title: FUS and TARDBP but not SOD1 interact in genetic models of amyotrophic lateral sclerosis.

The following tardbp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tardbp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36575	NM_201476.1 Latest version!	Danio rerio TAR DNA binding protein (tardbp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tardbp ( NM_201476.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_958884.1 Danio rerio TAR DNA binding protein (tardbp), mRNA.

Price & Availability↑

CloneID	ODa36575
Clone ID Related Accession (Same CDS sequence)	NM_201476.1
Accession Version	NM_201476.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-24
Organism	Danio rerio(zebrafish)
Product	TAR DNA-binding protein 43
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC049348.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC049348.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476760, SAMEA4476794 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGAGA TGTACATTCG AGTTGCGGAG GAGGAGAATG AGGAGCCAAT GGAGATCCCG 
TCAGAGGATG ATGGCACGGT TTTGCTTTCT ACAGTGTCCG CTCAGTTTCC AGGGGCTTGT 
GGTCTGCGCT TCCGGAGCCC AGTGTCTCAG TGCATGAGGG GGGTTCGTCT GGTGGACGGC 
ATTCTGCATG CACCTGAGAA CGGCTGGGGA AACCTAGTCT ATGTGGTTAA TTACCCAAAA 
GAAACTGTTC TGCCAGATAA TAAGAGGAAG ATGGATGAGA TCGATGCTTC ATCTGCGACC 
AAGATCAAGA GAGGAGATCA GAAGACTTCA GATCTGATTG TGCTGGGTCT GCCATGGAAG 
ACTTCAGAGC AAGACTTAAA AGACTACTTC GGTACATTTG GGGAAGTCAT CATGGTGCAG 
GTCAAGCGGG ATGTGAAGAC AGGAAATTCA AAAGGGTTTG GCTTTGTGAG GTTTGGAGAC 
TGGGAGACTC AGAGTAAGGT GATGACACAG CGGCACATGA TTGATGGCCG GTGGTGCGAC 
TGTAAACTGC CCAACTCAAA GCAAGGTATA GATGAACCAA TGAGGAGCCG TAAAGTGTTT 
GTGGGCCGCT GCACAGAGGA CATGACTGCC GATGAGCTCC GTCAGTTCTT CATGCAGTAC 
GGAGAGGTCA CAGACGTCTT TATTCCCAAA CCGTTCAGAG CGTTTGCTTT TGTCACCTTT 
GCAGATGACC AGGTTGCCGC CGCTTTGTGT GGAGAAGATC TGATCATCAA GGGCGTCAGT 
GTGCATATCT CAAACGCTGA GCCCAAACAC AATAACACTA GGCAGATGAT GGAGCGGGCA 
GGGCGCTTTG GGAATGGGTT CGGAGGTCAG GGTTTTGCAG GCAGCCGAAG CAACATGGGT 
GGTGGTGGTG GGGGTAGCTC CAGCAGCTTG GGAAATTTTG GCAATTTCAA TCTAAACCCG 
GCCATGATGG CTGCCGCCCA GGCTGCCTTG CAGAGTAGTT GGGGTATGAT GGGAATGCTA 
GCTCAGCAGA ATCAGTCGGG TACTTCAGGC ACAAGCACAA GTGGCACCAG TTCCTCTCGA 
GACCAAGCCC AAACATATAG CTCGGCTAAC AGCAATTACG GCAGCAGCTC AGCTGCTCTC 
GGCTGGGGCA CCGGCTCTAA CTCGGGCGCT GCCAGTGCTG GCTTTAACTC CAGTTTTGGC 
TCTAGTATGG AGTCCAAGTC ATCGGGGTGG GGTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_958884.1
CDS	247..1485
Translation	MAEMYIRVAEEENEEPMEIPSEDDGTVLLSTVSAQFPGACGLRFRSPVSQCMRGVRLVDGILHAPENGWGNLVYVVNYPKETVLPDNKRKMDEIDASSATKIKRGDQKTSDLIVLGLPWKTSEQDLKDYFGTFGEVIMVQVKRDVKTGNSKGFGFVRFGDWETQSKVMTQRHMIDGRWCDCKLPNSKQGIDEPMRSRKVFVGRCTEDMTADELRQFFMQYGEVTDVFIPKPFRAFAFVTFADDQVAAALCGEDLIIKGVSVHISNAEPKHNNTRQMMERAGRFGNGFGGQGFAGSRSNMGGGGGGSSSSLGNFGNFNLNPAMMAAAQAALQSSWGMMGMLAQQNQSGTSGTSTSGTSSSRDQAQTYSSANSNYGSSSAALGWGTGSNSGAASAGFNSSFGSSMESKSSGWGM

Target ORF information:

RefSeq Version	NM_201476.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TAR DNA binding protein (tardbp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201476.1

ORF Insert Sequence:

ATGGCCGAGA TGTACATTCG AGTTGCGGAG GAGGAGAATG AGGAGCCAAT GGAGATCCCG 
TCAGAGGATG ATGGCACGGT TTTGCTTTCT ACAGTGTCCG CTCAGTTTCC AGGGGCTTGT 
GGTCTGCGCT TCCGGAGCCC AGTGTCTCAG TGCATGAGGG GGGTTCGTCT GGTGGACGGC 
ATTCTGCATG CACCTGAGAA CGGCTGGGGA AACCTAGTCT ATGTGGTTAA TTACCCAAAA 
GAAACTGTTC TGCCAGATAA TAAGAGGAAG ATGGATGAGA TCGATGCTTC ATCTGCGACC 
AAGATCAAGA GAGGAGATCA GAAGACTTCA GATCTGATTG TGCTGGGTCT GCCATGGAAG 
ACTTCAGAGC AAGACTTAAA AGACTACTTC GGTACATTTG GGGAAGTCAT CATGGTGCAG 
GTCAAGCGGG ATGTGAAGAC AGGAAATTCA AAAGGGTTTG GCTTTGTGAG GTTTGGAGAC 
TGGGAGACTC AGAGTAAGGT GATGACACAG CGGCACATGA TTGATGGCCG GTGGTGCGAC 
TGTAAACTGC CCAACTCAAA GCAAGGTATA GATGAACCAA TGAGGAGCCG TAAAGTGTTT 
GTGGGCCGCT GCACAGAGGA CATGACTGCC GATGAGCTCC GTCAGTTCTT CATGCAGTAC 
GGAGAGGTCA CAGACGTCTT TATTCCCAAA CCGTTCAGAG CGTTTGCTTT TGTCACCTTT 
GCAGATGACC AGGTTGCCGC CGCTTTGTGT GGAGAAGATC TGATCATCAA GGGCGTCAGT 
GTGCATATCT CAAACGCTGA GCCCAAACAC AATAACACTA GGCAGATGAT GGAGCGGGCA 
GGGCGCTTTG GGAATGGGTT CGGAGGTCAG GGTTTTGCAG GCAGCCGAAG CAACATGGGT 
GGTGGTGGTG GGGGTAGCTC CAGCAGCTTG GGAAATTTTG GCAATTTCAA TCTAAACCCG 
GCCATGATGG CTGCCGCCCA GGCTGCCTTG CAGAGTAGTT GGGGTATGAT GGGAATGCTA 
GCTCAGCAGA ATCAGTCGGG TACTTCAGGC ACAAGCACAA GTGGCACCAG TTCCTCTCGA 
GACCAAGCCC AAACATATAG CTCGGCTAAC AGCAATTACG GCAGCAGCTC AGCTGCTCTC 
GGCTGGGGCA CCGGCTCTAA CTCGGGCGCT GCCAGTGCTG GCTTTAACTC CAGTTTTGGC 
TCTAGTATGG AGTCCAAGTC ATCGGGGTGG GGTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dynamic RNA-protein interactions underlie the zebrafish maternal-to-zygotic transition.
Genome research27(7)1184-1194(2017 Jul)
Despic V,Dejung M,Gu M,Krishnan J,Zhang J,Herzel L,Straube K,Gerstein MB,Butter F,Neugebauer KM

Augmented quantal release of acetylcholine at the vertebrate neuromuscular junction following tdp-43 depletion.
PloS one12(5)e0177005(2017)
Dzieciolowska S,Drapeau P,Armstrong GAB

Neurotrophic effects of progranulin in vivo in reversing motor neuron defects caused by over or under expression of TDP-43 or FUS.
PloS one12(3)e0174784(2017)
Chitramuthu BP,Kay DG,Bateman A,Bennett HP