gnai2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gnai2b
Entrez Gene ID	327650
Full Name	guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2b
Synonyms	fi21e06,gnai2,wu:fi21e06,zgc:56690
General protein information	Preferred Names guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2b Names guanine nucleotide-binding protein G(i) subunit alpha-2 Galpha i2a guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 guanine nucleotide-binding protein Gi2 alpha-subunit
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017356445.1	XP_017211934.1	guanine nucleotide-binding protein G(i) subunit alpha-2 isoform X1
NM_199842.1	NP_956136.1	guanine nucleotide-binding protein G(i) subunit alpha-2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	GNAI2	NP_990733.1
Canis lupus familiaris (dog)	GNAI2	NP_001003364.1
Mus musculus (house mouse)	Gnai2	NP_032164.2
Danio rerio (zebrafish)	gnai2b	NP_956136.1
Saccharomyces cerevisiae (baker's yeast)	GPA1	NP_011868.1
Xenopus tropicalis (tropical clawed frog)	gnai2	NP_989250.1
Homo sapiens (human)	GNAI2	NP_002061.1
Pan troglodytes (chimpanzee)	GNAI2	XP_001167942.1
	GNAI2	XP_001103122.1
Bos taurus (cattle)	GNAI2	NP_001094510.1
Magnaporthe oryzae (rice blast fungus)	MGG_00365	XP_003718715.1
Rattus norvegicus (Norway rat)	Gnai2	NP_112297.1
Kluyveromyces lactis	KLLA0F25916g	XP_456234.1
Eremothecium gossypii	AGOS_ADR153C	NP_984250.1
Neurospora crassa	NCU06493	XP_957133.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of Molecular Targets for 4,5-Dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) in Teleosts: New Insight into Mechanism of Toxicity.
Chen L, Au DW, Hu C, Peterson DR, Zhou B, Qian PY
Environmental science & technology51(3)1840-1847(2017 Feb)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The vertebrate ancestral repertoire of visual opsins, transducin alpha subunits and oxytocin/vasopressin receptors was established by duplication of their shared genomic region in the two rounds of early vertebrate genome duplications.
Lagman D, Ocampo Daza D, Widmark J, Abalo XM, Sundstr?m G, Larhammar D
BMC evolutionary biology13238(2013 Nov)

Mutually exclusive expression of G?ia and G?14 reveals diversification of taste receptor cells in zebrafish.
Ohmoto M, Okada S, Nakamura S, Abe K, Matsumoto I
The Journal of comparative neurology519(8)1616-29(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gnai2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gnai2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33842	NM_199842.1 Latest version!	Danio rerio guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2b (gnai2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa59340	XM_017356445.1 Latest version!	Danio rerio guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2b (gnai2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gnai2b ( NM_199842.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956136.1 Danio rerio guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2b (gnai2b), mRNA.

Price & Availability↑

CloneID	ODa33842
Clone ID Related Accession (Same CDS sequence)	NM_199842.1
Accession Version	NM_199842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-05
Organism	Danio rerio(zebrafish)
Product	guanine nucleotide-binding protein G(i) subunit alpha-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC049343.1. ##Evidence-Data-START## Transcript exon combination :: BC049343.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGGCTGCA CGGTGAGCGC GGAGGACAAG GCGGCCGCAG AGAGATCAAA GATGATCGAC 
AAAAACCTGC GGGAGGTGAA GCTGCTCCTG CTCGGTGCGG GTGAATCAGG GAAGAGCACC 
ATTGTCAAAC AGATGAAAAT CATTCATGAA GACGGATACT CAGAAGACGA ATGCAAGCAG 
TACCGAGCCG TGGTTTACAG CAACACCATC CAGTCCATAA TGGCCATCAT TAAAGCCATG 
AGCAACCTGA AGATCGACTA CGGGGAAAGT GCACGTGTGG ATGATGCGCG GCAACTGTTT 
GCGCTGTCTG CGGCTGCGGA GGAGCAGGGG ATCCTGACTG ACGATCTGGC CAATGTGATC 
CGCAGACTGT GGGCCGATAG CGGCGTTCAG GGCTGCTTCT CCAGATCCAG AGAATATCAG 
CTCAATGACT CCGCTGCTTA CTATCTGAAT GATCTGGACA GAATAGCGCG CTCAGACTAC 
ATTCCCACCC AGCAGGACGT GTTGAGGACC CGAGTGAAAA CCACAGGCAT CGTGGAGACT 
CACTTCACCT TTAAAGACCT GCACTTCAAG ATGTTTGATG TCGGAGGTCA AAGGTCAGAG 
AGGAAGAAGT GGATTCACTG TTTTGAAGGA GTGACGGCCA TCATCTTCTG TGTGGCTCTG 
AGCGCTTATG ACCTGGTGCT GGCAGAAGAC GAGGAGATGA ACCGCATGCA TGAAAGCATG 
AAGCTGTTCG ACTCCATCTG TAACAACAAG TGGTTCACCG AGACCTCCAT CATCCTCTTC 
CTCAACAAGA AAGATCTGTT CGAGGAGAAG ATCACGCGCA GCCCACTCAC CATCTGCTTT 
CCGGAATATT CAGGAGCCAA TAAATACGAC GAGGCTGCCA GTTACATCCA GACCAAGTTC 
GAGGATCTCA ACAAGAAAAA GGACACCAAG GAGATTTACA CCCACTTCAC CTGTGCTACT 
GACACTAAGA ACGTGCAGTT TGTGTTTGAC GCTGTTACTG ACGTCATTAT CAAGAACAAC 
CTGAAGGACT GTGGCCTCTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956136.1
CDS	35..1078
Translation	MGCTVSAEDKAAAERSKMIDKNLREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYRAVVYSNTIQSIMAIIKAMSNLKIDYGESARVDDARQLFALSAAAEEQGILTDDLANVIRRLWADSGVQGCFSRSREYQLNDSAAYYLNDLDRIARSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKKDLFEEKITRSPLTICFPEYSGANKYDEAASYIQTKFEDLNKKKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Target ORF information:

RefSeq Version	NM_199842.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2b (gnai2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199842.1

ORF Insert Sequence:

ATGGGCTGCA CGGTGAGCGC GGAGGACAAG GCGGCCGCAG AGAGATCAAA GATGATCGAC 
AAAAACCTGC GGGAGGTGAA GCTGCTCCTG CTCGGTGCGG GTGAATCAGG GAAGAGCACC 
ATTGTCAAAC AGATGAAAAT CATTCATGAA GACGGATACT CAGAAGACGA ATGCAAGCAG 
TACCGAGCCG TGGTTTACAG CAACACCATC CAGTCCATAA TGGCCATCAT TAAAGCCATG 
AGCAACCTGA AGATCGACTA CGGGGAAAGT GCACGTGTGG ATGATGCGCG GCAACTGTTT 
GCGCTGTCTG CGGCTGCGGA GGAGCAGGGG ATCCTGACTG ACGATCTGGC CAATGTGATC 
CGCAGACTGT GGGCCGATAG CGGCGTTCAG GGCTGCTTCT CCAGATCCAG AGAATATCAG 
CTCAATGACT CCGCTGCTTA CTATCTGAAT GATCTGGACA GAATAGCGCG CTCAGACTAC 
ATTCCCACCC AGCAGGACGT GTTGAGGACC CGAGTGAAAA CCACAGGCAT CGTGGAGACT 
CACTTCACCT TTAAAGACCT GCACTTCAAG ATGTTTGATG TCGGAGGTCA AAGGTCAGAG 
AGGAAGAAGT GGATTCACTG TTTTGAAGGA GTGACGGCCA TCATCTTCTG TGTGGCTCTG 
AGCGCTTATG ACCTGGTGCT GGCAGAAGAC GAGGAGATGA ACCGCATGCA TGAAAGCATG 
AAGCTGTTCG ACTCCATCTG TAACAACAAG TGGTTCACCG AGACCTCCAT CATCCTCTTC 
CTCAACAAGA AAGATCTGTT CGAGGAGAAG ATCACGCGCA GCCCACTCAC CATCTGCTTT 
CCGGAATATT CAGGAGCCAA TAAATACGAC GAGGCTGCCA GTTACATCCA GACCAAGTTC 
GAGGATCTCA ACAAGAAAAA GGACACCAAG GAGATTTACA CCCACTTCAC CTGTGCTACT 
GACACTAAGA ACGTGCAGTT TGTGTTTGAC GCTGTTACTG ACGTCATTAT CAAGAACAAC 
CTGAAGGACT GTGGCCTCTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gnai2b ( XM_017356445.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211934.1 Danio rerio guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2b (gnai2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa59340
Clone ID Related Accession (Same CDS sequence)	XM_017356445.1
Accession Version	XM_017356445.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	guanine nucleotide-binding protein G(i) subunit alpha-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATCA TTCATGAAGA CGGATACTCA GAAGACGAAT GCAAGCAGTA CCGAGCCGTG 
GTTTACAGCA ACACCATCCA GTCCATAATG GCCATCATTA AAGCCATGAG CAACCTGAAG 
ATCGACTACG GGGAAAGTGC ACGTGTGGAT GATGCGCGGC AACTGTTTGC GCTGTCTGCG 
GCTGCGGAGG AGCAGGGGAT CCTGACTGAC GATCTGGCCA ATGTGATCCG CAGACTGTGG 
GCCGATAGCG GCGTTCAGGG CTGCTTCTCC AGATCCAGAG AATATCAGCT CAATGACTCC 
GCTGCTTACT ATCTGAATGA TCTGGACAGA ATAGCGCGCT CAGACTACAT TCCCACCCAG 
CAGGACGTGT TGAGGACCCG AGTGAAAACC ACAGGCATCG TGGAGACTCA CTTCACCTTC 
AAAGACCTGC ACTTCAAGAT GTTTGATGTC GGAGGTCAAA GGTCAGAGAG GAAGAAGTGG 
ATTCACTGTT TTGAAGGAGT GACGGCCATC ATCTTCTGTG TGGCTCTGAG CGCTTATGAC 
CTGGTGCTGG CAGAAGACGA GGAGATGAAC CGCATGCATG AAAGCATGAA GCTGTTCGAC 
TCCATCTGTA ACAACAAGTG GTTCACCGAG ACCTCCATCA TCCTCTTCCT CAACAAGAAA 
GATCTGTTCG AGGAGAAGAT CACGCGCAGC CCACTCACCA TCTGCTTTCC AGAATATTCA 
GGAGCCAATA AATACGACGA GGCTGCCAGT TACATCCAGA CCAAGTTCGA GGATCTCAAC 
AAGAAAAAGG ACACCAAGGA GATTTACACC CACTTCACCT GTGCTACTGA CACTAAGAAC 
GTGCAGTTTG TGTTTGACGC CGTTACTGAC GTCATTATCA AGAACAACCT GAAGGACTGT 
GGCCTCTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211934.1
CDS	131..1042
Translation	MKIIHEDGYSEDECKQYRAVVYSNTIQSIMAIIKAMSNLKIDYGESARVDDARQLFALSAAAEEQGILTDDLANVIRRLWADSGVQGCFSRSREYQLNDSAAYYLNDLDRIARSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKKDLFEEKITRSPLTICFPEYSGANKYDEAASYIQTKFEDLNKKKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Target ORF information:

RefSeq Version	XM_017356445.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2b (gnai2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356445.1

ORF Insert Sequence:

ATGAAAATCA TTCATGAAGA CGGATACTCA GAAGACGAAT GCAAGCAGTA CCGAGCCGTG 
GTTTACAGCA ACACCATCCA GTCCATAATG GCCATCATTA AAGCCATGAG CAACCTGAAG 
ATCGACTACG GGGAAAGTGC ACGTGTGGAT GATGCGCGGC AACTGTTTGC GCTGTCTGCG 
GCTGCGGAGG AGCAGGGGAT CCTGACTGAC GATCTGGCCA ATGTGATCCG CAGACTGTGG 
GCCGATAGCG GCGTTCAGGG CTGCTTCTCC AGATCCAGAG AATATCAGCT CAATGACTCC 
GCTGCTTACT ATCTGAATGA TCTGGACAGA ATAGCGCGCT CAGACTACAT TCCCACCCAG 
CAGGACGTGT TGAGGACCCG AGTGAAAACC ACAGGCATCG TGGAGACTCA CTTCACCTTC 
AAAGACCTGC ACTTCAAGAT GTTTGATGTC GGAGGTCAAA GGTCAGAGAG GAAGAAGTGG 
ATTCACTGTT TTGAAGGAGT GACGGCCATC ATCTTCTGTG TGGCTCTGAG CGCTTATGAC 
CTGGTGCTGG CAGAAGACGA GGAGATGAAC CGCATGCATG AAAGCATGAA GCTGTTCGAC 
TCCATCTGTA ACAACAAGTG GTTCACCGAG ACCTCCATCA TCCTCTTCCT CAACAAGAAA 
GATCTGTTCG AGGAGAAGAT CACGCGCAGC CCACTCACCA TCTGCTTTCC AGAATATTCA 
GGAGCCAATA AATACGACGA GGCTGCCAGT TACATCCAGA CCAAGTTCGA GGATCTCAAC 
AAGAAAAAGG ACACCAAGGA GATTTACACC CACTTCACCT GTGCTACTGA CACTAAGAAC 
GTGCAGTTTG TGTTTGACGC CGTTACTGAC GTCATTATCA AGAACAACCT GAAGGACTGT 
GGCCTCTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of Molecular Targets for 4,5-Dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) in Teleosts: New Insight into Mechanism of Toxicity.
Environmental science & technology51(3)1840-1847(2017 Feb)
Chen L,Au DW,Hu C,Peterson DR,Zhou B,Qian PY

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The vertebrate ancestral repertoire of visual opsins, transducin alpha subunits and oxytocin/vasopressin receptors was established by duplication of their shared genomic region in the two rounds of early vertebrate genome duplications.
BMC evolutionary biology13238(2013 Nov)
Lagman D,Ocampo Daza D,Widmark J,Abalo XM,Sundstr?m G,Larhammar D

Mutually exclusive expression of G?ia and G?14 reveals diversification of taste receptor cells in zebrafish.
The Journal of comparative neurology519(8)1616-29(2011 Jun)
Ohmoto M,Okada S,Nakamura S,Abe K,Matsumoto I