dad1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dad1
Entrez Gene ID	566527
Full Name	defender against cell death 1
Synonyms	si:ch211-9d9.5
General protein information	Preferred Names defender against cell death 1 Names dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001100439.1	NP_001093909.1	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_05758	XP_003710680.1
Arabidopsis thaliana (thale cress)	ATDAD1	NP_174500.1
Arabidopsis thaliana (thale cress)	DAD2	NP_850247.1
Homo sapiens (human)	DAD1	NP_001335.1
Pan troglodytes (chimpanzee)	DAD1	XP_509838.2
Oryza sativa (rice)	Os04g0397000	NP_001052664.1
Canis lupus familiaris (dog)	DAD1	XP_537361.2
Danio rerio (zebrafish)	dad1	NP_001093909.1
Drosophila melanogaster (fruit fly)	l(2)k12914	NP_609222.1
Xenopus tropicalis (tropical clawed frog)	dad1	NP_001016940.1
	DAD1	XP_001099561.1
Bos taurus (cattle)	DAD1	NP_001029933.1
Mus musculus (house mouse)	Dad1	NP_034145.1
Rattus norvegicus (Norway rat)	Dad1	NP_620265.1
Gallus gallus (chicken)	DAD1	NP_001007474.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008491	XP_316953.2
Caenorhabditis elegans (roundworm)	dad-1	NP_491889.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification and expression of a new Ly6 gene cluster in zebrafish Danio rerio, with implications of being involved in embryonic immunity.
Wang M, Li L, Guo Q, Zhang S, Ji D, Li H
Fish & shellfish immunology54230-40(2016 Jul)

XBP-1, a key regulator of unfolded protein response, activates transcription of IGF1 and Akt phosphorylation in zebrafish embryonic cell line.
Hu MC, Gong HY, Lin GH, Hu SY, Chen MH, Huang SJ, Liao CF, Wu JL
Biochemical and biophysical research communications359(3)778-83(2007 Aug)

A shifted repertoire of endocannabinoid genes in the zebrafish (Danio rerio).
McPartland JM, Glass M, Matias I, Norris RW, Kilpatrick CW
Molecular genetics and genomics : MGG277(5)555-70(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dad1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dad1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35447	NM_001100439.1 Latest version!	Danio rerio defender against cell death 1 (dad1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dad1 ( NM_001100439.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001093909.1 Danio rerio defender against cell death 1 (dad1), mRNA.

Price & Availability↑

CloneID	ODa35447
Clone ID Related Accession (Same CDS sequence)	NM_001100439.1
Accession Version	NM_001100439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-02
Organism	Danio rerio(zebrafish)
Product	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC150399.1. On Aug 3, 2007 this sequence version replaced XM_689800.2. ##Evidence-Data-START## Transcript exon combination :: BC150399.1, EH437533.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476791 [ECO:0000348] ##Evidence-Data-END##

ATGTCTAATT CGGTGTTTTC CGTCATATCG CGTTTTGTAG AGGAATACAG AAGCAGCACG 
CTGACTAAGC TGAAGGTCAT CGACGCGTAT CTGTTGTATA TTTTGCTGAC CGGAGTCTTC 
CAGTTCTTGT ACTGTCTGCT GGTGGGAACC TTCCCCTTCA ACAGCTTCTT ATCTGGCTTC 
ATTTCATGTG TGGGGTCCTT CATACTGGCT GTGTGTCTTC GCATCCAGAT CAACCCTCAG 
AATAAAGGAG ATTTCCTCAC GGTCTCTCCT GAAAGAGCGT TCGCTGATTT CCTTTTTGCT 
CACACTGTTC TCCATCTAGT GGTGGTCAAT TTTGTGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093909.1
CDS	58..399
Translation	MSNSVFSVISRFVEEYRSSTLTKLKVIDAYLLYILLTGVFQFLYCLLVGTFPFNSFLSGFISCVGSFILAVCLRIQINPQNKGDFLTVSPERAFADFLFAHTVLHLVVVNFVG

Target ORF information:

RefSeq Version	NM_001100439.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio defender against cell death 1 (dad1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100439.1

ORF Insert Sequence:

ATGTCTAATT CGGTGTTTTC CGTCATATCG CGTTTTGTAG AGGAATACAG AAGCAGCACG 
CTGACTAAGC TGAAGGTCAT CGACGCGTAT CTGTTGTATA TTTTGCTGAC CGGAGTCTTC 
CAGTTCTTGT ACTGTCTGCT GGTGGGAACC TTCCCCTTCA ACAGCTTCTT ATCTGGCTTC 
ATTTCATGTG TGGGGTCCTT CATACTGGCT GTGTGTCTTC GCATCCAGAT CAACCCTCAG 
AATAAAGGAG ATTTCCTCAC GGTCTCTCCT GAAAGAGCGT TCGCTGATTT CCTTTTTGCT 
CACACTGTTC TCCATCTAGT GGTGGTCAAT TTTGTGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification and expression of a new Ly6 gene cluster in zebrafish Danio rerio, with implications of being involved in embryonic immunity.
Fish & shellfish immunology54230-40(2016 Jul)
Wang M,Li L,Guo Q,Zhang S,Ji D,Li H

XBP-1, a key regulator of unfolded protein response, activates transcription of IGF1 and Akt phosphorylation in zebrafish embryonic cell line.
Biochemical and biophysical research communications359(3)778-83(2007 Aug)
Hu MC,Gong HY,Lin GH,Hu SY,Chen MH,Huang SJ,Liao CF,Wu JL

A shifted repertoire of endocannabinoid genes in the zebrafish (Danio rerio).
Molecular genetics and genomics : MGG277(5)555-70(2007 May)
McPartland JM,Glass M,Matias I,Norris RW,Kilpatrick CW