spg7 cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following spg7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spg7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa13515 XM_001923083.6
Latest version!
Danio rerio spastic paraplegia 7 (spg7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa144950 XM_021474143.1
Latest version!
Danio rerio spastic paraplegia 7 (spg7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa144950 XM_021472024.1
Latest version!
Danio rerio spastic paraplegia 7 (spg7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
ODa13515 XM_001923083.5 Danio rerio spastic paraplegia 7 (spg7), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa13515
    Clone ID Related Accession (Same CDS sequence) XM_001923083.6 , XM_001923083.5
    Accession Version XM_001923083.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2367bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product paraplegin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001923083.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCAGCGC TACTTCTGCA GCGTGGAAAC ACATGTTATA GCAAACGGTT TTGGTCGCTG 
    TCGTCTCGTT TTAGCTGTTT TCAGAACAGT AGAAAGCTAA TAAAGAGCAA TGTGACGCCT
    TTAGTGATTC CAAAGACACG AGGGCTAAAC TCAGGACACT TTCAGGACAT TTTGACAAAG
    CCTTTAATCC CAAGGCTTAT TGGACTTGAA TTCCATTGTC GCCACAACTT GCTCACAACT
    CAAGCGAAAC TTTGGAAACT TTTAGGCAGT ACTCATTACT TCAGCACATC CAACAGAAAA
    CATGAAAACA AACAGGGTGG AAAAGGGAAG ACCCCTGAGG AAGAGGAAGA AGAGAAAAAG
    AGACGAGAAC AGGAGGATCA GATGTACAGG GAACGACTGC GCACACTCTT TATTATCGCT
    GTTATCATGA GCTTACTCAA CTCTATTAAC ACCAGTGGCG GTAACATATC CTGGAACGAT
    TTTGTGAATG AAATGTTGGC CAAAGGGGAG GTGTCGCGAG TACAGGTGGT CCCAGAGAGT
    GACATTGTGG AGATCTACCT TCACCCTGGA GCTGTCATCT TCGGCAGACC GAGACTTGCC
    CTGATGTATC GAATGCAGGT GGCCAACATT GACAAGTTTG AGGAGAAGCT TAGAGCGGCG
    GAGGAAGAGC TGAATATAGA TGCCAAAGAC AGAATACCAG TGACCTACAA GCGCACAGGC
    TTCTTTGGGA ATGCCCTTTA TGCTCTTGGC ATGGCTGCCA TTGGGGTTGC AATCCTCTGG
    TACATCTTCC GATTAGCAGG AATGGGTGGG AGAGACGGAG GCTTTAGTGC CTTTAATCAG
    TTGAAAATGG CTAAATTCAC CATTGTTGAT GGGAAGTCTG GGAAAGGTGT TAGCTTCAAA
    GATGTGGCAG GAATGCGCGA GGCCAAGATG GAAGTCAAAG AGTTTGTGGA TTATCTCAAG
    AATCCAGACC GATACCTTCA GCTTGGGGCG AAGGTTCCGA AAGGCTCTCT GCTTCTGGGA
    CCTCCTGGCT GTGGGAAGAC TCTGCTTGCT AAGGCTGTGG CAACGGAGGC TCAGGTGCCC
    TTTCTGGCTA TGGCAGGTTC AGAGTTTGTA GAGGTGATTG GAGGCCTTGG AGCTGCAAGG
    GTGAGAAGTC TTTTTAAAGA GGCTCGTGCT CGAGCTCCTT GCATTGTGTA CATCGATGAG
    ATTGATGCCG TGGGGAAGAA ACGCTCCACA AACATGTCTG GTTTCTCAAA CACAGAGGAG
    GAGCAGACCC TAAACCAGCT ACTTGTGGAG ATGGATGGAA TGGGTACAAC AGATCATGTT
    ATTGTCTTGG CTTCCACTAA TCGGGCAGAT ATTCTGGACA ACGCGCTCAT GAGGCCAGGC
    AGGCTTGATA GGCACATATT CATAGACCTG CCAACACTTC AGGAGAGAAA GGAAATCTTT
    GAGCAGCACC TGAAAATCCT AAAGCTCACA CAACCAGCAG ACTTTTATTC TCTGCGCCTG
    GCTGAGTTGA CACCAGGCTT CAGTGGTGCT GACATTGCCA ACATCTGTAA TGAAGCTGCC
    CTTCATGCTG CCAGAGAGGG CTTCAAGTCC ATCGACACCT TCAGCTTTGA ATATGCTGTG
    GAAAGAGTCA TTGCAGGGAG TGTGAAGAAG AGTAAAATCT TATCGAAGGA GGAGCAGAGG
    GTGGTAGCCT TCCATGAATC TGGTCATGCT CTAGTTGGAT GGCTGCTAGA ACACACAGAA
    GCTGTCATGA AGGTATCAAT AGCTCCCAGG ACAAATGCTG CTTTGGGCTT TGCTCAGATC
    CTGCCGAGGG ATCAGTTTTT GTTCACTAAA GAGCAGCTGT TTGAGAGGAT GTGCATGGCA
    TTGGGTGGAC GAGCCTCTGA GGCCATCACC TTCAATAAGG TCACCACAGG TGCTCAGGAC
    GACCTGCGGA AGGTGACCCG TGTAGCGTAC TCCATGGTGA AGCAGTATGG GATGGTTGAC
    AGTGTGGGTC AGGTGTCATT CCCTGACTCT GAGAATCAGA GTGGCATTGG ACGAAGACCC
    TTCAGCCAAG GCCTCCAGCA GCAGATGGAC CTCGAAGCTA AGATGCTAAT AGCAAAAGCC
    TACAGGCATA CAGAGAAACT GCTTTTGGAC AACAGAGACA AACTTATTTT GCTGGCCAAT
    GCTCTGCTAG AGAGAGAGGT GGTGAACTAC AATGACATTG AGGCTCTACT GGGGCCTCCT
    CCATTTGGCC CCAAAAGGAT GATTGCTCCT CAGAGCTGGG TGGAGGCTGA ACGAGACCTG
    CAGGACACAG GAGAAGATGA GCCTCGCAGA CCCCAGAGAC CCTCCAAAGA CAACAAAGAT
    GACGACATCA ACTTGAGCCC AGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001923118.1
    CDS346..2712
    Translation

    Target ORF information:

    RefSeq Version XM_001923083.6
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spastic paraplegia 7 (spg7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001923083.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCAGCGC TACTTCTGCA GCGTGGAAAC ACATGTTATA GCAAACGGTT TTGGTCGCTG 
    TCGTCTCGTT TTAGCTGTTT TCAGAACAGT AGAAAGCTAA TAAAGAGCAA TGTGACGCCT
    TTAGTGATTC CAAAGACACG AGGGCTAAAC TCAGGACACT TTCAGGACAT TTTGACAAAG
    CCTTTAATCC CAAGGCTTAT TGGACTTGAA TTCCATTGTC GCCACAACTT GCTCACAACT
    CAAGCGAAAC TTTGGAAACT TTTAGGCAGT ACTCATTACT TCAGCACATC CAACAGAAAA
    CATGAAAACA AACAGGGTGG AAAAGGGAAG ACCCCTGAGG AAGAGGAAGA AGAGAAAAAG
    AGACGAGAAC AGGAGGATCA GATGTACAGG GAACGACTGC GCACACTCTT TATTATCGCT
    GTTATCATGA GCTTACTCAA CTCTATTAAC ACCAGTGGCG GTAACATATC CTGGAACGAT
    TTTGTGAATG AAATGTTGGC CAAAGGGGAG GTGTCGCGAG TACAGGTGGT CCCAGAGAGT
    GACATTGTGG AGATCTACCT TCACCCTGGA GCTGTCATCT TCGGCAGACC GAGACTTGCC
    CTGATGTATC GAATGCAGGT GGCCAACATT GACAAGTTTG AGGAGAAGCT TAGAGCGGCG
    GAGGAAGAGC TGAATATAGA TGCCAAAGAC AGAATACCAG TGACCTACAA GCGCACAGGC
    TTCTTTGGGA ATGCCCTTTA TGCTCTTGGC ATGGCTGCCA TTGGGGTTGC AATCCTCTGG
    TACATCTTCC GATTAGCAGG AATGGGTGGG AGAGACGGAG GCTTTAGTGC CTTTAATCAG
    TTGAAAATGG CTAAATTCAC CATTGTTGAT GGGAAGTCTG GGAAAGGTGT TAGCTTCAAA
    GATGTGGCAG GAATGCGCGA GGCCAAGATG GAAGTCAAAG AGTTTGTGGA TTATCTCAAG
    AATCCAGACC GATACCTTCA GCTTGGGGCG AAGGTTCCGA AAGGCTCTCT GCTTCTGGGA
    CCTCCTGGCT GTGGGAAGAC TCTGCTTGCT AAGGCTGTGG CAACGGAGGC TCAGGTGCCC
    TTTCTGGCTA TGGCAGGTTC AGAGTTTGTA GAGGTGATTG GAGGCCTTGG AGCTGCAAGG
    GTGAGAAGTC TTTTTAAAGA GGCTCGTGCT CGAGCTCCTT GCATTGTGTA CATCGATGAG
    ATTGATGCCG TGGGGAAGAA ACGCTCCACA AACATGTCTG GTTTCTCAAA CACAGAGGAG
    GAGCAGACCC TAAACCAGCT ACTTGTGGAG ATGGATGGAA TGGGTACAAC AGATCATGTT
    ATTGTCTTGG CTTCCACTAA TCGGGCAGAT ATTCTGGACA ACGCGCTCAT GAGGCCAGGC
    AGGCTTGATA GGCACATATT CATAGACCTG CCAACACTTC AGGAGAGAAA GGAAATCTTT
    GAGCAGCACC TGAAAATCCT AAAGCTCACA CAACCAGCAG ACTTTTATTC TCTGCGCCTG
    GCTGAGTTGA CACCAGGCTT CAGTGGTGCT GACATTGCCA ACATCTGTAA TGAAGCTGCC
    CTTCATGCTG CCAGAGAGGG CTTCAAGTCC ATCGACACCT TCAGCTTTGA ATATGCTGTG
    GAAAGAGTCA TTGCAGGGAG TGTGAAGAAG AGTAAAATCT TATCGAAGGA GGAGCAGAGG
    GTGGTAGCCT TCCATGAATC TGGTCATGCT CTAGTTGGAT GGCTGCTAGA ACACACAGAA
    GCTGTCATGA AGGTATCAAT AGCTCCCAGG ACAAATGCTG CTTTGGGCTT TGCTCAGATC
    CTGCCGAGGG ATCAGTTTTT GTTCACTAAA GAGCAGCTGT TTGAGAGGAT GTGCATGGCA
    TTGGGTGGAC GAGCCTCTGA GGCCATCACC TTCAATAAGG TCACCACAGG TGCTCAGGAC
    GACCTGCGGA AGGTGACCCG TGTAGCGTAC TCCATGGTGA AGCAGTATGG GATGGTTGAC
    AGTGTGGGTC AGGTGTCATT CCCTGACTCT GAGAATCAGA GTGGCATTGG ACGAAGACCC
    TTCAGCCAAG GCCTCCAGCA GCAGATGGAC CTCGAAGCTA AGATGCTAAT AGCAAAAGCC
    TACAGGCATA CAGAGAAACT GCTTTTGGAC AACAGAGACA AACTTATTTT GCTGGCCAAT
    GCTCTGCTAG AGAGAGAGGT GGTGAACTAC AATGACATTG AGGCTCTACT GGGGCCTCCT
    CCATTTGGCC CCAAAAGGAT GATTGCTCCT CAGAGCTGGG TGGAGGCTGA ACGAGACCTG
    CAGGACACAG GAGAAGATGA GCCTCGCAGA CCCCAGAGAC CCTCCAAAGA CAACAAAGAT
    GACGACATCA ACTTGAGCCC AGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13515
    Clone ID Related Accession (Same CDS sequence) XM_001923083.6 , XM_001923083.5
    Accession Version XM_001923083.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2367bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product paraplegin
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001923083.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCAGCGC TACTTCTGCA GCGTGGAAAC ACATGTTATA GCAAACGGTT TTGGTCGCTG 
    TCGTCTCGTT TTAGCTGTTT TCAGAACAGT AGAAAGCTAA TAAAGAGCAA TGTGACGCCT
    TTAGTGATTC CAAAGACACG AGGGCTAAAC TCAGGACACT TTCAGGACAT TTTGACAAAG
    CCTTTAATCC CAAGGCTTAT TGGACTTGAA TTCCATTGTC GCCACAACTT GCTCACAACT
    CAAGCGAAAC TTTGGAAACT TTTAGGCAGT ACTCATTACT TCAGCACATC CAACAGAAAA
    CATGAAAACA AACAGGGTGG AAAAGGGAAG ACCCCTGAGG AAGAGGAAGA AGAGAAAAAG
    AGACGAGAAC AGGAGGATCA GATGTACAGG GAACGACTGC GCACACTCTT TATTATCGCT
    GTTATCATGA GCTTACTCAA CTCTATTAAC ACCAGTGGCG GTAACATATC CTGGAACGAT
    TTTGTGAATG AAATGTTGGC CAAAGGGGAG GTGTCGCGAG TACAGGTGGT CCCAGAGAGT
    GACATTGTGG AGATCTACCT TCACCCTGGA GCTGTCATCT TCGGCAGACC GAGACTTGCC
    CTGATGTATC GAATGCAGGT GGCCAACATT GACAAGTTTG AGGAGAAGCT TAGAGCGGCG
    GAGGAAGAGC TGAATATAGA TGCCAAAGAC AGAATACCAG TGACCTACAA GCGCACAGGC
    TTCTTTGGGA ATGCCCTTTA TGCTCTTGGC ATGGCTGCCA TTGGGGTTGC AATCCTCTGG
    TACATCTTCC GATTAGCAGG AATGGGTGGG AGAGACGGAG GCTTTAGTGC CTTTAATCAG
    TTGAAAATGG CTAAATTCAC CATTGTTGAT GGGAAGTCTG GGAAAGGTGT TAGCTTCAAA
    GATGTGGCAG GAATGCGCGA GGCCAAGATG GAAGTCAAAG AGTTTGTGGA TTATCTCAAG
    AATCCAGACC GATACCTTCA GCTTGGGGCG AAGGTTCCGA AAGGCTCTCT GCTTCTGGGA
    CCTCCTGGCT GTGGGAAGAC TCTGCTTGCT AAGGCTGTGG CAACGGAGGC TCAGGTGCCC
    TTTCTGGCTA TGGCAGGTTC AGAGTTTGTA GAGGTGATTG GAGGCCTTGG AGCTGCAAGG
    GTGAGAAGTC TTTTTAAAGA GGCTCGTGCT CGAGCTCCTT GCATTGTGTA CATCGATGAG
    ATTGATGCCG TGGGGAAGAA ACGCTCCACA AACATGTCTG GTTTCTCAAA CACAGAGGAG
    GAGCAGACCC TAAACCAGCT ACTTGTGGAG ATGGATGGAA TGGGTACAAC AGATCATGTT
    ATTGTCTTGG CTTCCACTAA TCGGGCAGAT ATTCTGGACA ACGCGCTCAT GAGGCCAGGC
    AGGCTTGATA GGCACATATT CATAGACCTG CCAACACTTC AGGAGAGAAA GGAAATCTTT
    GAGCAGCACC TGAAAATCCT AAAGCTCACA CAACCAGCAG ACTTTTATTC TCTGCGCCTG
    GCTGAGTTGA CACCAGGCTT CAGTGGTGCT GACATTGCCA ACATCTGTAA TGAAGCTGCC
    CTTCATGCTG CCAGAGAGGG CTTCAAGTCC ATCGACACCT TCAGCTTTGA ATATGCTGTG
    GAAAGAGTCA TTGCAGGGAG TGTGAAGAAG AGTAAAATCT TATCGAAGGA GGAGCAGAGG
    GTGGTAGCCT TCCATGAATC TGGTCATGCT CTAGTTGGAT GGCTGCTAGA ACACACAGAA
    GCTGTCATGA AGGTATCAAT AGCTCCCAGG ACAAATGCTG CTTTGGGCTT TGCTCAGATC
    CTGCCGAGGG ATCAGTTTTT GTTCACTAAA GAGCAGCTGT TTGAGAGGAT GTGCATGGCA
    TTGGGTGGAC GAGCCTCTGA GGCCATCACC TTCAATAAGG TCACCACAGG TGCTCAGGAC
    GACCTGCGGA AGGTGACCCG TGTAGCGTAC TCCATGGTGA AGCAGTATGG GATGGTTGAC
    AGTGTGGGTC AGGTGTCATT CCCTGACTCT GAGAATCAGA GTGGCATTGG ACGAAGACCC
    TTCAGCCAAG GCCTCCAGCA GCAGATGGAC CTCGAAGCTA AGATGCTAAT AGCAAAAGCC
    TACAGGCATA CAGAGAAACT GCTTTTGGAC AACAGAGACA AACTTATTTT GCTGGCCAAT
    GCTCTGCTAG AGAGAGAGGT GGTGAACTAC AATGACATTG AGGCTCTACT GGGGCCTCCT
    CCATTTGGCC CCAAAAGGAT GATTGCTCCT CAGAGCTGGG TGGAGGCTGA ACGAGACCTG
    CAGGACACAG GAGAAGATGA GCCTCGCAGA CCCCAGAGAC CCTCCAAAGA CAACAAAGAT
    GACGACATCA ACTTGAGCCC AGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001923118.1
    CDS340..2706
    Translation

    Target ORF information:

    RefSeq Version XM_001923083.5
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spastic paraplegia 7 (spg7), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001923083.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCAGCGC TACTTCTGCA GCGTGGAAAC ACATGTTATA GCAAACGGTT TTGGTCGCTG 
    TCGTCTCGTT TTAGCTGTTT TCAGAACAGT AGAAAGCTAA TAAAGAGCAA TGTGACGCCT
    TTAGTGATTC CAAAGACACG AGGGCTAAAC TCAGGACACT TTCAGGACAT TTTGACAAAG
    CCTTTAATCC CAAGGCTTAT TGGACTTGAA TTCCATTGTC GCCACAACTT GCTCACAACT
    CAAGCGAAAC TTTGGAAACT TTTAGGCAGT ACTCATTACT TCAGCACATC CAACAGAAAA
    CATGAAAACA AACAGGGTGG AAAAGGGAAG ACCCCTGAGG AAGAGGAAGA AGAGAAAAAG
    AGACGAGAAC AGGAGGATCA GATGTACAGG GAACGACTGC GCACACTCTT TATTATCGCT
    GTTATCATGA GCTTACTCAA CTCTATTAAC ACCAGTGGCG GTAACATATC CTGGAACGAT
    TTTGTGAATG AAATGTTGGC CAAAGGGGAG GTGTCGCGAG TACAGGTGGT CCCAGAGAGT
    GACATTGTGG AGATCTACCT TCACCCTGGA GCTGTCATCT TCGGCAGACC GAGACTTGCC
    CTGATGTATC GAATGCAGGT GGCCAACATT GACAAGTTTG AGGAGAAGCT TAGAGCGGCG
    GAGGAAGAGC TGAATATAGA TGCCAAAGAC AGAATACCAG TGACCTACAA GCGCACAGGC
    TTCTTTGGGA ATGCCCTTTA TGCTCTTGGC ATGGCTGCCA TTGGGGTTGC AATCCTCTGG
    TACATCTTCC GATTAGCAGG AATGGGTGGG AGAGACGGAG GCTTTAGTGC CTTTAATCAG
    TTGAAAATGG CTAAATTCAC CATTGTTGAT GGGAAGTCTG GGAAAGGTGT TAGCTTCAAA
    GATGTGGCAG GAATGCGCGA GGCCAAGATG GAAGTCAAAG AGTTTGTGGA TTATCTCAAG
    AATCCAGACC GATACCTTCA GCTTGGGGCG AAGGTTCCGA AAGGCTCTCT GCTTCTGGGA
    CCTCCTGGCT GTGGGAAGAC TCTGCTTGCT AAGGCTGTGG CAACGGAGGC TCAGGTGCCC
    TTTCTGGCTA TGGCAGGTTC AGAGTTTGTA GAGGTGATTG GAGGCCTTGG AGCTGCAAGG
    GTGAGAAGTC TTTTTAAAGA GGCTCGTGCT CGAGCTCCTT GCATTGTGTA CATCGATGAG
    ATTGATGCCG TGGGGAAGAA ACGCTCCACA AACATGTCTG GTTTCTCAAA CACAGAGGAG
    GAGCAGACCC TAAACCAGCT ACTTGTGGAG ATGGATGGAA TGGGTACAAC AGATCATGTT
    ATTGTCTTGG CTTCCACTAA TCGGGCAGAT ATTCTGGACA ACGCGCTCAT GAGGCCAGGC
    AGGCTTGATA GGCACATATT CATAGACCTG CCAACACTTC AGGAGAGAAA GGAAATCTTT
    GAGCAGCACC TGAAAATCCT AAAGCTCACA CAACCAGCAG ACTTTTATTC TCTGCGCCTG
    GCTGAGTTGA CACCAGGCTT CAGTGGTGCT GACATTGCCA ACATCTGTAA TGAAGCTGCC
    CTTCATGCTG CCAGAGAGGG CTTCAAGTCC ATCGACACCT TCAGCTTTGA ATATGCTGTG
    GAAAGAGTCA TTGCAGGGAG TGTGAAGAAG AGTAAAATCT TATCGAAGGA GGAGCAGAGG
    GTGGTAGCCT TCCATGAATC TGGTCATGCT CTAGTTGGAT GGCTGCTAGA ACACACAGAA
    GCTGTCATGA AGGTATCAAT AGCTCCCAGG ACAAATGCTG CTTTGGGCTT TGCTCAGATC
    CTGCCGAGGG ATCAGTTTTT GTTCACTAAA GAGCAGCTGT TTGAGAGGAT GTGCATGGCA
    TTGGGTGGAC GAGCCTCTGA GGCCATCACC TTCAATAAGG TCACCACAGG TGCTCAGGAC
    GACCTGCGGA AGGTGACCCG TGTAGCGTAC TCCATGGTGA AGCAGTATGG GATGGTTGAC
    AGTGTGGGTC AGGTGTCATT CCCTGACTCT GAGAATCAGA GTGGCATTGG ACGAAGACCC
    TTCAGCCAAG GCCTCCAGCA GCAGATGGAC CTCGAAGCTA AGATGCTAAT AGCAAAAGCC
    TACAGGCATA CAGAGAAACT GCTTTTGGAC AACAGAGACA AACTTATTTT GCTGGCCAAT
    GCTCTGCTAG AGAGAGAGGT GGTGAACTAC AATGACATTG AGGCTCTACT GGGGCCTCCT
    CCATTTGGCC CCAAAAGGAT GATTGCTCCT CAGAGCTGGG TGGAGGCTGA ACGAGACCTG
    CAGGACACAG GAGAAGATGA GCCTCGCAGA CCCCAGAGAC CCTCCAAAGA CAACAAAGAT
    GACGACATCA ACTTGAGCCC AGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144950
    Clone ID Related Accession (Same CDS sequence) XM_021472024.1 , XM_021474143.1
    Accession Version XM_021472024.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product paraplegin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394712.1) and transcript sequence (GDQQ01008843.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 632 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCATGACC TTGGTGTTCC TTCAAAGATA AACCTATCTG TAATTATTTC ACAGGACATT 
    TTGACAAAGC CTTTAATCCC AAGGCTTATT GGACTTGAAT TCCATTGTCG CCACAACTTG
    CTCCCAACTC AAGCAAAACT TTGGAAACTT TTAGGCAGTA CTCATTACTT CAGCACATCC
    AACAGAAAAC ATGAAAACAA ACAGGGTGGA AAAGGGAAGA CCCCTGAGGA AGAGGAAGAA
    GAGAAAAAGA GACGAGAACA GGAGGATCAG ATGTACAGGG AACGACTGCG CACACTCTTT
    ATTATCGCTG TTATCATGAG CTTACTCAAC TCTATTAACA CCAGTGGCGG TAACATATCC
    TGGAACGATT TTGTGAATGA AATGTTGGCC AAAGGGGAGG TGTCGCGAGT ACAGGTGGTC
    CCAGAGAGTG ACATTGTGGA GATCTACCTT CACCCTGGAG CTGTCATCTT CGGCAGACCG
    AGACTTGCCC TGATGTATCG AATGCAGGTG GCCAACATTG ACAAGTTTGA GGAGAAGCTT
    AGAGCGGCGG AGGAAGAGCT GAATATAGAT GCCAAAGACA GAATACCAGT GACCTACAAG
    CGCACAGGCT TCTTTGGGAA TGCCCTTTAT GCTCTTGGCA TGGCTGCCAT TGGGGTTGCA
    ATCCTCTGGT ACATCTTCCG ATTAGCAGGA ATGGGTGGGA GAGACGGAGG CTTTAGTGCC
    TTTAATCAGT TGAAAATGGC TAAATTCACC ATTGTTGATG GGAAGTCTGG GAAAGGTGTT
    AGCTTCAAAG ATGTGGCAGG AATGCGCGAG GCCAAGATGG AAGTCAAAGA GTTTGTGGAT
    TATCTCAAGA ATCCAGACCG ATACCTTCAG CTTGGGGCGA AGGTTCCGAA AGGCTCTCTG
    CTTCTGGGAC CTCCTGGCTG TGGGAAGACT CTGCTTGCTA AGGCTGTGGC AACGGAGGCT
    CAGGTGCCCT TTCTGGCTAT GGCAGGTTCA GAGTTTGTAG AGGTGATTGG AGGCCTTGGA
    GCTGCAAGGG TGAGAAGTCT TTTTAAAGAG GCTCGTGCTC GAGCTCCTTG CATTGTGTAC
    ATCGATGAGA TTGATGCCGT GGGGAAGAAA CGCTCCACAA ACATGTCTGG TTTCTCAAAC
    ACAGAGGAGG AGCAGACCCT AAACCAGCTA CTTGTGGAGA TGGATGGAAT GGGTACAACA
    GATCATGTTA TTGTCTTGGC TTCCACTAAT CGGGCAGATA TTCTGGACAA CGCGCTCATG
    AGGCCAGGCA GGCTTGATAG GCACATATTC ATAGACCTGC CAACACTTCA GGAGAGAAAG
    GAAATCTTTG AGCAGCACCT GAAAATCCTA AAGCTCACAC AACCAGCAGA CTTTTATTCT
    CTGCGCCTGG CTGAGTTGAC ACCAGGCTTC AGTGGTGCTG ACATTGCCAA CATCTGTAAT
    GAAGCTGCCC TTCATGCTGC CAGAGAGGGC TTCAAGTCCA TCGACACCTT CAGCTTTGAA
    TATGCTGTGG AAAGAGTCAT TGCAGGGAGT GTGAAGAAGA GTAAAATCTT ATCGAAGGAG
    GAGCAGAGGG TGGTAGCCTT CCATGAATCT GGTCATGCTC TAGTTGGATG GCTGCTAGAA
    CACACAGAAG CTGTCATGAA GGTATCAATA GCTCCCAGGA CAAATGCTGC TTTGGGCTTT
    GCTCAGATCC TGCCGAGGGA TCAGTTTTTG TTCACTAAAG AGCAGCTGTT TGAGAGGATG
    TGCATGGCAT TGGGTGGACG AGCCTCTGAG GCCATCACCT TCAATAAGGT CACCACAGGT
    GCTCAGGACG ACCTGCGGAA GGTGACCCGT GTAGCGTACT CCATGGTGAA GCAGTATGGG
    ATGGTTGACA GTGTGGGTCA GGTGTCATTC CCTGACTCTG AGAATCAGAG TGGCATTGGA
    CGAAGACCCT TCAGCCAAGG CCTCCAGCAG CAGATGGACC TCGAAGCTAA GATGCTAATA
    GCAAAAGCCT ACAGGCATAC AGAGAAACTG CTTTTGGACA ACAGAGACAA ACTTATTTTG
    CTGGCCAATG CTCTGCTAGA GAGAGAGGTG GTGAACTACA ATGACATTGA GGCTCTACTG
    GGGCCTCCTC CATTTGGCCC CAAAAGGATG ATTGCTCCTC AGAGCTGGGT GGAGGCTGAA
    CGAGACCTGC AGGACACAGG AGAAGATGAG CCTCGCAGAC CCCAGAGACC CTCCAAAGAC
    AACAAAGATG ACGACATCAA CTTGAGCCCA GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327699.1
    CDS395..2650
    Translation

    Target ORF information:

    RefSeq Version XM_021472024.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spastic paraplegia 7 (spg7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021472024.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCATGACC TTGGTGTTCC TTCAAAGATA AACCTATCTG TAATTATTTC ACAGGACATT 
    TTGACAAAGC CTTTAATCCC AAGGCTTATT GGACTTGAAT TCCATTGTCG CCACAACTTG
    CTCCCAACTC AAGCAAAACT TTGGAAACTT TTAGGCAGTA CTCATTACTT CAGCACATCC
    AACAGAAAAC ATGAAAACAA ACAGGGTGGA AAAGGGAAGA CCCCTGAGGA AGAGGAAGAA
    GAGAAAAAGA GACGAGAACA GGAGGATCAG ATGTACAGGG AACGACTGCG CACACTCTTT
    ATTATCGCTG TTATCATGAG CTTACTCAAC TCTATTAACA CCAGTGGCGG TAACATATCC
    TGGAACGATT TTGTGAATGA AATGTTGGCC AAAGGGGAGG TGTCGCGAGT ACAGGTGGTC
    CCAGAGAGTG ACATTGTGGA GATCTACCTT CACCCTGGAG CTGTCATCTT CGGCAGACCG
    AGACTTGCCC TGATGTATCG AATGCAGGTG GCCAACATTG ACAAGTTTGA GGAGAAGCTT
    AGAGCGGCGG AGGAAGAGCT GAATATAGAT GCCAAAGACA GAATACCAGT GACCTACAAG
    CGCACAGGCT TCTTTGGGAA TGCCCTTTAT GCTCTTGGCA TGGCTGCCAT TGGGGTTGCA
    ATCCTCTGGT ACATCTTCCG ATTAGCAGGA ATGGGTGGGA GAGACGGAGG CTTTAGTGCC
    TTTAATCAGT TGAAAATGGC TAAATTCACC ATTGTTGATG GGAAGTCTGG GAAAGGTGTT
    AGCTTCAAAG ATGTGGCAGG AATGCGCGAG GCCAAGATGG AAGTCAAAGA GTTTGTGGAT
    TATCTCAAGA ATCCAGACCG ATACCTTCAG CTTGGGGCGA AGGTTCCGAA AGGCTCTCTG
    CTTCTGGGAC CTCCTGGCTG TGGGAAGACT CTGCTTGCTA AGGCTGTGGC AACGGAGGCT
    CAGGTGCCCT TTCTGGCTAT GGCAGGTTCA GAGTTTGTAG AGGTGATTGG AGGCCTTGGA
    GCTGCAAGGG TGAGAAGTCT TTTTAAAGAG GCTCGTGCTC GAGCTCCTTG CATTGTGTAC
    ATCGATGAGA TTGATGCCGT GGGGAAGAAA CGCTCCACAA ACATGTCTGG TTTCTCAAAC
    ACAGAGGAGG AGCAGACCCT AAACCAGCTA CTTGTGGAGA TGGATGGAAT GGGTACAACA
    GATCATGTTA TTGTCTTGGC TTCCACTAAT CGGGCAGATA TTCTGGACAA CGCGCTCATG
    AGGCCAGGCA GGCTTGATAG GCACATATTC ATAGACCTGC CAACACTTCA GGAGAGAAAG
    GAAATCTTTG AGCAGCACCT GAAAATCCTA AAGCTCACAC AACCAGCAGA CTTTTATTCT
    CTGCGCCTGG CTGAGTTGAC ACCAGGCTTC AGTGGTGCTG ACATTGCCAA CATCTGTAAT
    GAAGCTGCCC TTCATGCTGC CAGAGAGGGC TTCAAGTCCA TCGACACCTT CAGCTTTGAA
    TATGCTGTGG AAAGAGTCAT TGCAGGGAGT GTGAAGAAGA GTAAAATCTT ATCGAAGGAG
    GAGCAGAGGG TGGTAGCCTT CCATGAATCT GGTCATGCTC TAGTTGGATG GCTGCTAGAA
    CACACAGAAG CTGTCATGAA GGTATCAATA GCTCCCAGGA CAAATGCTGC TTTGGGCTTT
    GCTCAGATCC TGCCGAGGGA TCAGTTTTTG TTCACTAAAG AGCAGCTGTT TGAGAGGATG
    TGCATGGCAT TGGGTGGACG AGCCTCTGAG GCCATCACCT TCAATAAGGT CACCACAGGT
    GCTCAGGACG ACCTGCGGAA GGTGACCCGT GTAGCGTACT CCATGGTGAA GCAGTATGGG
    ATGGTTGACA GTGTGGGTCA GGTGTCATTC CCTGACTCTG AGAATCAGAG TGGCATTGGA
    CGAAGACCCT TCAGCCAAGG CCTCCAGCAG CAGATGGACC TCGAAGCTAA GATGCTAATA
    GCAAAAGCCT ACAGGCATAC AGAGAAACTG CTTTTGGACA ACAGAGACAA ACTTATTTTG
    CTGGCCAATG CTCTGCTAGA GAGAGAGGTG GTGAACTACA ATGACATTGA GGCTCTACTG
    GGGCCTCCTC CATTTGGCCC CAAAAGGATG ATTGCTCCTC AGAGCTGGGT GGAGGCTGAA
    CGAGACCTGC AGGACACAGG AGAAGATGAG CCTCGCAGAC CCCAGAGACC CTCCAAAGAC
    AACAAAGATG ACGACATCAA CTTGAGCCCA GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144950
    Clone ID Related Accession (Same CDS sequence) XM_021472024.1 , XM_021474143.1
    Accession Version XM_021474143.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product paraplegin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395269.1) and transcript sequence (GDQQ01008843.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 632 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCATGACC TTGGTGTTCC TTCAAAGATA AACCTATCTG TAATTATTTC ACAGGACATT 
    TTGACAAAGC CTTTAATCCC AAGGCTTATT GGACTTGAAT TCCATTGTCG CCACAACTTG
    CTCCCAACTC AAGCAAAACT TTGGAAACTT TTAGGCAGTA CTCATTACTT CAGCACATCC
    AACAGAAAAC ATGAAAACAA ACAGGGTGGA AAAGGGAAGA CCCCTGAGGA AGAGGAAGAA
    GAGAAAAAGA GACGAGAACA GGAGGATCAG ATGTACAGGG AACGACTGCG CACACTCTTT
    ATTATCGCTG TTATCATGAG CTTACTCAAC TCTATTAACA CCAGTGGCGG TAACATATCC
    TGGAACGATT TTGTGAATGA AATGTTGGCC AAAGGGGAGG TGTCGCGAGT ACAGGTGGTC
    CCAGAGAGTG ACATTGTGGA GATCTACCTT CACCCTGGAG CTGTCATCTT CGGCAGACCG
    AGACTTGCCC TGATGTATCG AATGCAGGTG GCCAACATTG ACAAGTTTGA GGAGAAGCTT
    AGAGCGGCGG AGGAAGAGCT GAATATAGAT GCCAAAGACA GAATACCAGT GACCTACAAG
    CGCACAGGCT TCTTTGGGAA TGCCCTTTAT GCTCTTGGCA TGGCTGCCAT TGGGGTTGCA
    ATCCTCTGGT ACATCTTCCG ATTAGCAGGA ATGGGTGGGA GAGACGGAGG CTTTAGTGCC
    TTTAATCAGT TGAAAATGGC TAAATTCACC ATTGTTGATG GGAAGTCTGG GAAAGGTGTT
    AGCTTCAAAG ATGTGGCAGG AATGCGCGAG GCCAAGATGG AAGTCAAAGA GTTTGTGGAT
    TATCTCAAGA ATCCAGACCG ATACCTTCAG CTTGGGGCGA AGGTTCCGAA AGGCTCTCTG
    CTTCTGGGAC CTCCTGGCTG TGGGAAGACT CTGCTTGCTA AGGCTGTGGC AACGGAGGCT
    CAGGTGCCCT TTCTGGCTAT GGCAGGTTCA GAGTTTGTAG AGGTGATTGG AGGCCTTGGA
    GCTGCAAGGG TGAGAAGTCT TTTTAAAGAG GCTCGTGCTC GAGCTCCTTG CATTGTGTAC
    ATCGATGAGA TTGATGCCGT GGGGAAGAAA CGCTCCACAA ACATGTCTGG TTTCTCAAAC
    ACAGAGGAGG AGCAGACCCT AAACCAGCTA CTTGTGGAGA TGGATGGAAT GGGTACAACA
    GATCATGTTA TTGTCTTGGC TTCCACTAAT CGGGCAGATA TTCTGGACAA CGCGCTCATG
    AGGCCAGGCA GGCTTGATAG GCACATATTC ATAGACCTGC CAACACTTCA GGAGAGAAAG
    GAAATCTTTG AGCAGCACCT GAAAATCCTA AAGCTCACAC AACCAGCAGA CTTTTATTCT
    CTGCGCCTGG CTGAGTTGAC ACCAGGCTTC AGTGGTGCTG ACATTGCCAA CATCTGTAAT
    GAAGCTGCCC TTCATGCTGC CAGAGAGGGC TTCAAGTCCA TCGACACCTT CAGCTTTGAA
    TATGCTGTGG AAAGAGTCAT TGCAGGGAGT GTGAAGAAGA GTAAAATCTT ATCGAAGGAG
    GAGCAGAGGG TGGTAGCCTT CCATGAATCT GGTCATGCTC TAGTTGGATG GCTGCTAGAA
    CACACAGAAG CTGTCATGAA GGTATCAATA GCTCCCAGGA CAAATGCTGC TTTGGGCTTT
    GCTCAGATCC TGCCGAGGGA TCAGTTTTTG TTCACTAAAG AGCAGCTGTT TGAGAGGATG
    TGCATGGCAT TGGGTGGACG AGCCTCTGAG GCCATCACCT TCAATAAGGT CACCACAGGT
    GCTCAGGACG ACCTGCGGAA GGTGACCCGT GTAGCGTACT CCATGGTGAA GCAGTATGGG
    ATGGTTGACA GTGTGGGTCA GGTGTCATTC CCTGACTCTG AGAATCAGAG TGGCATTGGA
    CGAAGACCCT TCAGCCAAGG CCTCCAGCAG CAGATGGACC TCGAAGCTAA GATGCTAATA
    GCAAAAGCCT ACAGGCATAC AGAGAAACTG CTTTTGGACA ACAGAGACAA ACTTATTTTG
    CTGGCCAATG CTCTGCTAGA GAGAGAGGTG GTGAACTACA ATGACATTGA GGCTCTACTG
    GGGCCTCCTC CATTTGGCCC CAAAAGGATG ATTGCTCCTC AGAGCTGGGT GGAGGCTGAA
    CGAGACCTGC AGGACACAGG AGAAGATGAG CCTCGCAGAC CCCAGAGACC CTCCAAAGAC
    AACAAAGATG ACGACATCAA CTTGAGCCCA GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329818.1
    CDS395..2650
    Translation

    Target ORF information:

    RefSeq Version XM_021474143.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spastic paraplegia 7 (spg7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474143.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCATGACC TTGGTGTTCC TTCAAAGATA AACCTATCTG TAATTATTTC ACAGGACATT 
    TTGACAAAGC CTTTAATCCC AAGGCTTATT GGACTTGAAT TCCATTGTCG CCACAACTTG
    CTCCCAACTC AAGCAAAACT TTGGAAACTT TTAGGCAGTA CTCATTACTT CAGCACATCC
    AACAGAAAAC ATGAAAACAA ACAGGGTGGA AAAGGGAAGA CCCCTGAGGA AGAGGAAGAA
    GAGAAAAAGA GACGAGAACA GGAGGATCAG ATGTACAGGG AACGACTGCG CACACTCTTT
    ATTATCGCTG TTATCATGAG CTTACTCAAC TCTATTAACA CCAGTGGCGG TAACATATCC
    TGGAACGATT TTGTGAATGA AATGTTGGCC AAAGGGGAGG TGTCGCGAGT ACAGGTGGTC
    CCAGAGAGTG ACATTGTGGA GATCTACCTT CACCCTGGAG CTGTCATCTT CGGCAGACCG
    AGACTTGCCC TGATGTATCG AATGCAGGTG GCCAACATTG ACAAGTTTGA GGAGAAGCTT
    AGAGCGGCGG AGGAAGAGCT GAATATAGAT GCCAAAGACA GAATACCAGT GACCTACAAG
    CGCACAGGCT TCTTTGGGAA TGCCCTTTAT GCTCTTGGCA TGGCTGCCAT TGGGGTTGCA
    ATCCTCTGGT ACATCTTCCG ATTAGCAGGA ATGGGTGGGA GAGACGGAGG CTTTAGTGCC
    TTTAATCAGT TGAAAATGGC TAAATTCACC ATTGTTGATG GGAAGTCTGG GAAAGGTGTT
    AGCTTCAAAG ATGTGGCAGG AATGCGCGAG GCCAAGATGG AAGTCAAAGA GTTTGTGGAT
    TATCTCAAGA ATCCAGACCG ATACCTTCAG CTTGGGGCGA AGGTTCCGAA AGGCTCTCTG
    CTTCTGGGAC CTCCTGGCTG TGGGAAGACT CTGCTTGCTA AGGCTGTGGC AACGGAGGCT
    CAGGTGCCCT TTCTGGCTAT GGCAGGTTCA GAGTTTGTAG AGGTGATTGG AGGCCTTGGA
    GCTGCAAGGG TGAGAAGTCT TTTTAAAGAG GCTCGTGCTC GAGCTCCTTG CATTGTGTAC
    ATCGATGAGA TTGATGCCGT GGGGAAGAAA CGCTCCACAA ACATGTCTGG TTTCTCAAAC
    ACAGAGGAGG AGCAGACCCT AAACCAGCTA CTTGTGGAGA TGGATGGAAT GGGTACAACA
    GATCATGTTA TTGTCTTGGC TTCCACTAAT CGGGCAGATA TTCTGGACAA CGCGCTCATG
    AGGCCAGGCA GGCTTGATAG GCACATATTC ATAGACCTGC CAACACTTCA GGAGAGAAAG
    GAAATCTTTG AGCAGCACCT GAAAATCCTA AAGCTCACAC AACCAGCAGA CTTTTATTCT
    CTGCGCCTGG CTGAGTTGAC ACCAGGCTTC AGTGGTGCTG ACATTGCCAA CATCTGTAAT
    GAAGCTGCCC TTCATGCTGC CAGAGAGGGC TTCAAGTCCA TCGACACCTT CAGCTTTGAA
    TATGCTGTGG AAAGAGTCAT TGCAGGGAGT GTGAAGAAGA GTAAAATCTT ATCGAAGGAG
    GAGCAGAGGG TGGTAGCCTT CCATGAATCT GGTCATGCTC TAGTTGGATG GCTGCTAGAA
    CACACAGAAG CTGTCATGAA GGTATCAATA GCTCCCAGGA CAAATGCTGC TTTGGGCTTT
    GCTCAGATCC TGCCGAGGGA TCAGTTTTTG TTCACTAAAG AGCAGCTGTT TGAGAGGATG
    TGCATGGCAT TGGGTGGACG AGCCTCTGAG GCCATCACCT TCAATAAGGT CACCACAGGT
    GCTCAGGACG ACCTGCGGAA GGTGACCCGT GTAGCGTACT CCATGGTGAA GCAGTATGGG
    ATGGTTGACA GTGTGGGTCA GGTGTCATTC CCTGACTCTG AGAATCAGAG TGGCATTGGA
    CGAAGACCCT TCAGCCAAGG CCTCCAGCAG CAGATGGACC TCGAAGCTAA GATGCTAATA
    GCAAAAGCCT ACAGGCATAC AGAGAAACTG CTTTTGGACA ACAGAGACAA ACTTATTTTG
    CTGGCCAATG CTCTGCTAGA GAGAGAGGTG GTGAACTACA ATGACATTGA GGCTCTACTG
    GGGCCTCCTC CATTTGGCCC CAAAAGGATG ATTGCTCCTC AGAGCTGGGT GGAGGCTGAA
    CGAGACCTGC AGGACACAGG AGAAGATGAG CCTCGCAGAC CCCAGAGACC CTCCAAAGAC
    AACAAAGATG ACGACATCAA CTTGAGCCCA GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.