• English
  • Sign In
    Please Sign In
    Sign In
    New to GenScript?
    Register
  • Contact Us
×
Contact us now: +1-877-436-7274

SPG7 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol SPG7
Entrez Gene ID 6687
Full Name SPG7, paraplegin matrix AAA peptidase subunit
Synonyms CAR,CMAR,PGN,SPG5C
General protein information
Preferred Names

SPG7, paraplegin matrix AAA peptidase subunit

Names

paraplegin
cell matrix adhesion regulator
spastic paraplegia 7 (pure and complicated autosomal recessive)
spastic paraplegia 7 protein
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

16

16q24.3
Summary This gene encodes a mitochondrial metalloprotease protein that is a member of the AAA family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutations in this gene cause autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2014].
Disorder MIM:

602783

Disorder Html: Spastic paraplegia 7, autosomal recessive, 607259 (3)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_017023598.1 XP_016879087.1 paraplegin isoform X3
XM_005256321.4 XP_005256378.1 paraplegin isoform X4
NM_001363850.1 NP_001350779.1 paraplegin isoform 3
NM_199367.3 NP_955399.1 paraplegin isoform 2 precursor
NM_003119.4 NP_003110.1 paraplegin isoform 1 precursor
XM_006721264.3 XP_006721327.1 paraplegin isoform X1
XM_006721264.4 XP_006721327.1 paraplegin isoform X1
XM_017023597.1 XP_016879086.1 paraplegin isoform X2
XM_017023599.1 XP_016879088.1 paraplegin isoform X4

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) SPG7 XP_001140249.3
SPG7 XP_002802648.1
Rattus norvegicus (Norway rat) Spg7 NP_852053.1
Danio rerio (zebrafish) spg7 XP_001923118.1
Gallus gallus (chicken) SPG7 NP_001012545.1
Drosophila melanogaster (fruit fly) CG2658 NP_570017.1
Caenorhabditis elegans (roundworm) ppgn-1 NP_500191.3
Mus musculus (house mouse) Spg7 NP_694816.3
Anopheles gambiae (African malaria mosquito) AgaP_AGAP000560 XP_310523.5
Homo sapiens (human) SPG7 NP_003110.1
Canis lupus familiaris (dog) SPG7 XP_546777.3
Bos taurus (cattle) SPG7 NP_001095492.1
Xenopus tropicalis (tropical clawed frog) spg7 XP_002933705.1

Gene Ontology

The following SPG7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPG7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu23601 NM_003119.4
Latest version!		 Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$279.50-$391.30
$559.00
OHu23719 NM_199367.3
Latest version!		 Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $286.30
$409.00
OHu49482 NM_001363850.1
Latest version!		 Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu23719 XM_017023599.1
Latest version!		 Homo sapiens SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $286.30
$409.00
OHu49482 XM_006721264.4
Latest version!		 Homo sapiens SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu49485 XM_005256321.4
Latest version!		 Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
OHu86234 XM_017023597.1
Latest version!		 Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu86235 XM_017023598.1
Latest version!		 Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
View Old Accession Versions >>
OHu49482 XM_006721264.3 Homo sapiens SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu23601
Clone ID Related Accession (Same CDS sequence) NM_003119.4
Accession Version NM_003119.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2388bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-04
Organism Homo sapiens(human)
Product paraplegin isoform 1 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY006239.1, Y16610.1 and BC036104.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_003119.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036104.1, SRR1660809.210110.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG 
CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG 
CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG 
AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT 
TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG 
CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC 
AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC 
GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC 
AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG 
ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG 
ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG 
GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA 
GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG 
GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG 
GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG 
GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC 
ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG 
GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT 
TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC 
ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG 
TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC 
ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG 
CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGGGACTGC CAAAAAGAGC 
AAGATCCTGT CCAAGGAAGA ACAGAAAGTG GTTGCGTTTC ATGAGTCGGG CCACGCCTTG 
GTGGGCTGGA TGCTGGAGCA CACGGAGGCC GTGATGAAGG TCTCCATAAC CCCTCGGACA 
AACGCCGCCC TGGGCTTTGC TCAGATGCTC CCCAGAGACC AGCACCTCTT CACCAAGGAG 
CAGCTGTTTG AGCGGATGTG CATGGCCCTG GGAGGACGGG CCTCGGAAGC ACTGTCCTTC 
AACGAGGTCA CTTCTGGGGC ACAGGACGAC CTGAGGAAGG TCACCCGCAT CGCCTACTCC 
ATGGTGAAGC AGTTTGGGAT GGCACCTGGC ATCGGGCCCA TCTCCTTCCC TGAGGCGCAG 
GAGGGCCTCA TGGGCATCGG GCGGCGCCCC TTCAGCCAAG GCCTGCAGCA GATGATGGAC 
CATGAAGCAA GACTGCTGGT GGCCAAGGCC TACAGACACA CCGAGAAGGT GCTGCAGGAC 
AACCTGGACA AGTTGCAGGC GCTGGCAAAC GCCCTTCTGG AAAAGGAAGT GATAAACTAT 
GAGGACATTG AGGCTCTCAT TGGCCCGCCG CCCCATGGGC CGAAGAAAAT GATCGCACCG 
CAGAGGTGGA TCGACGCCCA GAGGGAGAAA CAGGACTTGG GCGAGGAGGA GACCGAAGAG 
ACCCAGCAGC CTCCACTTGG AGGCGAAGAG CCGACTTGGC CCAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003110.1
CDS16..2403
Translation

Target ORF information:

RefSeq Version NM_003119.4
Organism Homo sapiens(human)
Definition Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_003119.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG 
CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG 
CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG 
AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT 
TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG 
CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC 
AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC 
GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC 
AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG 
ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG 
ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG 
GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA 
GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG 
GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG 
GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG 
GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC 
ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG 
GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT 
TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC 
ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG 
TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC 
ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG 
CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGGGACTGC CAAAAAGAGC 
AAGATCCTGT CCAAGGAAGA ACAGAAAGTG GTTGCGTTTC ATGAGTCGGG CCACGCCTTG 
GTGGGCTGGA TGCTGGAGCA CACGGAGGCC GTGATGAAGG TCTCCATAAC CCCTCGGACA 
AACGCCGCCC TGGGCTTTGC TCAGATGCTC CCCAGAGACC AGCACCTCTT CACCAAGGAG 
CAGCTGTTTG AGCGGATGTG CATGGCCCTG GGAGGACGGG CCTCGGAAGC ACTGTCCTTC 
AACGAGGTCA CTTCTGGGGC ACAGGACGAC CTGAGGAAGG TCACCCGCAT CGCCTACTCC 
ATGGTGAAGC AGTTTGGGAT GGCACCTGGC ATCGGGCCCA TCTCCTTCCC TGAGGCGCAG 
GAGGGCCTCA TGGGCATCGG GCGGCGCCCC TTCAGCCAAG GCCTGCAGCA GATGATGGAC 
CATGAAGCAA GACTGCTGGT GGCCAAGGCC TACAGACACA CCGAGAAGGT GCTGCAGGAC 
AACCTGGACA AGTTGCAGGC GCTGGCAAAC GCCCTTCTGG AAAAGGAAGT GATAAACTAT 
GAGGACATTG AGGCTCTCAT TGGCCCGCCG CCCCATGGGC CGAAGAAAAT GATCGCACCG 
CAGAGGTGGA TCGACGCCCA GAGGGAGAAA CAGGACTTGG GCGAGGAGGA GACCGAAGAG 
ACCCAGCAGC CTCCACTTGG AGGCGAAGAG CCGACTTGGC CCAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23719
Clone ID Related Accession (Same CDS sequence) NM_199367.3 , XM_017023599.1
Accession Version NM_199367.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1470bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-04
Organism Homo sapiens(human)
Product paraplegin isoform 2 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY006239.1, Y16610.1, BC007692.1 and CA447374.1. On Jun 2, 2019 this sequence version replaced NM_199367.2. Transcript Variant: This variant (2) lacks multiple 3' coding exons and contains an alternate 3' terminal exon, resulting in a different 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) contains a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007692.1, SRR1660803.205602.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG 
CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG 
CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG 
AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT 
TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG 
CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC 
AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC 
GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC 
AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG 
ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG 
ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG 
GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA 
GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG 
GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG 
GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG 
GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC 
ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG 
GATGGTGCCT CTCTTGACCA GCTACCCTCC CAGGGGACCA TGAGGAAGCT CAGAGGAAAG 
ACCCCCGCCT GCTCCTGTCT CACGGAGCCC ACAGGGTCAC GGAGGGCAAT GGAGGGTCAT 
TCGCTCTGCT GGGGTTGCCT TTTGCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_955399.1
CDS16..1485
Translation

Target ORF information:

RefSeq Version NM_199367.3
Organism Homo sapiens(human)
Definition Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_199367.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG 
CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG 
CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG 
AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT 
TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG 
CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC 
AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC 
GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC 
AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG 
ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG 
ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG 
GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA 
GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG 
GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG 
GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG 
GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC 
ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG 
GATGGTGCCT CTCTTGACCA GCTACCCTCC CAGGGGACCA TGAGGAAGCT CAGAGGAAAG 
ACCCCCGCCT GCTCCTGTCT CACGGAGCCC ACAGGGTCAC GGAGGGCAAT GGAGGGTCAT 
TCGCTCTGCT GGGGTTGCCT TTTGCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu49482
Clone ID Related Accession (Same CDS sequence) NM_001363850.1 , XM_006721264.3 , XM_006721264.4
Accession Version NM_001363850.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2430bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-01
Organism Homo sapiens(human)
Product paraplegin isoform 3
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY006239.1, Y16610.1, BC036104.1, AC092123.4 and BC052587.1. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG 
CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG 
CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG 
AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT 
TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG 
CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC 
AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC 
GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC 
AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG 
ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG 
ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG 
GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA 
GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG 
GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG 
GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG 
GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC 
ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG 
GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT 
TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC 
ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG 
TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC 
ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG 
CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGGGACTGC CAAAAAGAGC 
AAGATCCTGT CCAAGGAAGA ACAGAAAGTG GTTGCGTTTC ATGAGTCGGG CCACGCCTTG 
GTGGGCTGGA TGCTGGAGCA CACGGAGGCC GTGATGAAGG TCTCCATAAC CCCTCGGACA 
AACGCCGCCC TGGGCTTTGC TCAGATGCTC CCCAGAGACC AGCACCTCTT CACCAAGGAG 
CAGCTGTTTG AGCGGATGTG CATGGCCCTG GGAGGACGGG CCTCGGAAGC ACTGTCCTTC 
AACGAGGTCA CTTCTGGGGC ACAGGACGAC CTGAGGAAGG TCACCCGCAT CGCCTACTCC 
ATGGTGAAGC AGTTTGGGAT GGCACCTGGC ATCGGGCCCA TCTCCTTCCC TGAGGCGCAG 
GAGGGCCTCA TGGGCATCGG GCGGCGCCCC TTCAGCCAAG GCCTGCAGCA GATGATGGAC 
CATGAAGCAA GACTGCTGGT GGCCAAGGCC TACAGACACA CCGAGAAGGT GCTGCAGGAC 
AACCTGGACA AGTTGCAGGC GCGAGCACTG TGTGTCACGT GGGGATCGTG TTTGGGGCCC 
TTAGACTGTC GTCCCCAGCC AGCAGCAGCC GCCTCTGGGC AGGTGTGGGC GGTGCCGTCC 
CCGGCTGAAG CAGGCCTGCC TGTGGACCCA TGCTCATCAC GGTTGACAAA GCAGCTCTGC 
AGAGCTCGGG CACATCACAG TGGGAGCCAC AACGGGAGCA AAGCTGCAGC TGGCAAACGC 
CCTTCTGGAA AAGGAAGTGA TAAACTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001350779.1
CDS31..2460
Translation

Target ORF information:

RefSeq Version NM_001363850.1
Organism Homo sapiens(human)
Definition Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001363850.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG 
CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG 
CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG 
AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT 
TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG 
CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC 
AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC 
GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC 
AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG 
ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG 
ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG 
GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA 
GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG 
GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG 
GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG 
GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC 
ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG 
GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT 
TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC 
ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG 
TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC 
ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG 
CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGGGACTGC CAAAAAGAGC 
AAGATCCTGT CCAAGGAAGA ACAGAAAGTG GTTGCGTTTC ATGAGTCGGG CCACGCCTTG 
GTGGGCTGGA TGCTGGAGCA CACGGAGGCC GTGATGAAGG TCTCCATAAC CCCTCGGACA 
AACGCCGCCC TGGGCTTTGC TCAGATGCTC CCCAGAGACC AGCACCTCTT CACCAAGGAG 
CAGCTGTTTG AGCGGATGTG CATGGCCCTG GGAGGACGGG CCTCGGAAGC ACTGTCCTTC 
AACGAGGTCA CTTCTGGGGC ACAGGACGAC CTGAGGAAGG TCACCCGCAT CGCCTACTCC 
ATGGTGAAGC AGTTTGGGAT GGCACCTGGC ATCGGGCCCA TCTCCTTCCC TGAGGCGCAG 
GAGGGCCTCA TGGGCATCGG GCGGCGCCCC TTCAGCCAAG GCCTGCAGCA GATGATGGAC 
CATGAAGCAA GACTGCTGGT GGCCAAGGCC TACAGACACA CCGAGAAGGT GCTGCAGGAC 
AACCTGGACA AGTTGCAGGC GCGAGCACTG TGTGTCACGT GGGGATCGTG TTTGGGGCCC 
TTAGACTGTC GTCCCCAGCC AGCAGCAGCC GCCTCTGGGC AGGTGTGGGC GGTGCCGTCC 
CCGGCTGAAG CAGGCCTGCC TGTGGACCCA TGCTCATCAC GGTTGACAAA GCAGCTCTGC 
AGAGCTCGGG CACATCACAG TGGGAGCCAC AACGGGAGCA AAGCTGCAGC TGGCAAACGC 
CCTTCTGGAA AAGGAAGTGA TAAACTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23719
Clone ID Related Accession (Same CDS sequence) NM_199367.3 , XM_017023599.1
Accession Version XM_017023599.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1470bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product paraplegin isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG 
CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG 
CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG 
AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT 
TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG 
CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC 
AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC 
GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC 
AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG 
ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG 
ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG 
GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA 
GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG 
GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG 
GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG 
GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC 
ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG 
GATGGTGCCT CTCTTGACCA GCTACCCTCC CAGGGGACCA TGAGGAAGCT CAGAGGAAAG 
ACCCCCGCCT GCTCCTGTCT CACGGAGCCC ACAGGGTCAC GGAGGGCAAT GGAGGGTCAT 
TCGCTCTGCT GGGGTTGCCT TTTGCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016879088.1
CDS40..1509
Translation

Target ORF information:

RefSeq Version XM_017023599.1
Organism Homo sapiens(human)
Definition Homo sapiens SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017023599.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG 
CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG 
CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG 
AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT 
TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG 
CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC 
AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC 
GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC 
AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG 
ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG 
ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG 
GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA 
GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG 
GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG 
GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG 
GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC 
ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG 
GATGGTGCCT CTCTTGACCA GCTACCCTCC CAGGGGACCA TGAGGAAGCT CAGAGGAAAG 
ACCCCCGCCT GCTCCTGTCT CACGGAGCCC ACAGGGTCAC GGAGGGCAAT GGAGGGTCAT 
TCGCTCTGCT GGGGTTGCCT TTTGCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu49482
Clone ID Related Accession (Same CDS sequence) NM_001363850.1 , XM_006721264.3 , XM_006721264.4
Accession Version XM_006721264.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2430bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product paraplegin isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006721264.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG 
CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG 
CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG 
AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT 
TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG 
CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC 
AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC 
GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC 
AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG 
ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG 
ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG 
GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA 
GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG 
GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG 
GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG 
GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC 
ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG 
GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT 
TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC 
ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG 
TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC 
ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG 
CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGGGACTGC CAAAAAGAGC 
AAGATCCTGT CCAAGGAAGA ACAGAAAGTG GTTGCGTTTC ATGAGTCGGG CCACGCCTTG 
GTGGGCTGGA TGCTGGAGCA CACGGAGGCC GTGATGAAGG TCTCCATAAC CCCTCGGACA 
AACGCCGCCC TGGGCTTTGC TCAGATGCTC CCCAGAGACC AGCACCTCTT CACCAAGGAG 
CAGCTGTTTG AGCGGATGTG CATGGCCCTG GGAGGACGGG CCTCGGAAGC ACTGTCCTTC 
AACGAGGTCA CTTCTGGGGC ACAGGACGAC CTGAGGAAGG TCACCCGCAT CGCCTACTCC 
ATGGTGAAGC AGTTTGGGAT GGCACCTGGC ATCGGGCCCA TCTCCTTCCC TGAGGCGCAG 
GAGGGCCTCA TGGGCATCGG GCGGCGCCCC TTCAGCCAAG GCCTGCAGCA GATGATGGAC 
CATGAAGCAA GACTGCTGGT GGCCAAGGCC TACAGACACA CCGAGAAGGT GCTGCAGGAC 
AACCTGGACA AGTTGCAGGC GCGAGCACTG TGTGTCACGT GGGGATCGTG TTTGGGGCCC 
TTAGACTGTC GTCCCCAGCC AGCAGCAGCC GCCTCTGGGC AGGTGTGGGC GGTGCCGTCC 
CCGGCTGAAG CAGGCCTGCC TGTGGACCCA TGCTCATCAC GGTTGACAAA GCAGCTCTGC 
AGAGCTCGGG CACATCACAG TGGGAGCCAC AACGGGAGCA AAGCTGCAGC TGGCAAACGC 
CCTTCTGGAA AAGGAAGTGA TAAACTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006721327.1
CDS40..2469
Translation

Target ORF information:

RefSeq Version XM_006721264.3
Organism Homo sapiens(human)
Definition Homo sapiens SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006721264.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG 
CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG 
CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG 
AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT 
TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG 
CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC 
AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC 
GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC 
AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG 
ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG 
ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG 
GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA 
GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG 
GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG 
GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG 
GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC 
ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG 
GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT 
TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC 
ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG 
TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC 
ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG 
CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGGGACTGC CAAAAAGAGC 
AAGATCCTGT CCAAGGAAGA ACAGAAAGTG GTTGCGTTTC ATGAGTCGGG CCACGCCTTG 
GTGGGCTGGA TGCTGGAGCA CACGGAGGCC GTGATGAAGG TCTCCATAAC CCCTCGGACA 
AACGCCGCCC TGGGCTTTGC TCAGATGCTC CCCAGAGACC AGCACCTCTT CACCAAGGAG 
CAGCTGTTTG AGCGGATGTG CATGGCCCTG GGAGGACGGG CCTCGGAAGC ACTGTCCTTC 
AACGAGGTCA CTTCTGGGGC ACAGGACGAC CTGAGGAAGG TCACCCGCAT CGCCTACTCC 
ATGGTGAAGC AGTTTGGGAT GGCACCTGGC ATCGGGCCCA TCTCCTTCCC TGAGGCGCAG 
GAGGGCCTCA TGGGCATCGG GCGGCGCCCC TTCAGCCAAG GCCTGCAGCA GATGATGGAC 
CATGAAGCAA GACTGCTGGT GGCCAAGGCC TACAGACACA CCGAGAAGGT GCTGCAGGAC 
AACCTGGACA AGTTGCAGGC GCGAGCACTG TGTGTCACGT GGGGATCGTG TTTGGGGCCC 
TTAGACTGTC GTCCCCAGCC AGCAGCAGCC GCCTCTGGGC AGGTGTGGGC GGTGCCGTCC 
CCGGCTGAAG CAGGCCTGCC TGTGGACCCA TGCTCATCAC GGTTGACAAA GCAGCTCTGC 
AGAGCTCGGG CACATCACAG TGGGAGCCAC AACGGGAGCA AAGCTGCAGC TGGCAAACGC 
CCTTCTGGAA AAGGAAGTGA TAAACTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu49482
Clone ID Related Accession (Same CDS sequence) NM_001363850.1 , XM_006721264.3 , XM_006721264.4
Accession Version XM_006721264.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2430bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-03-25
Organism Homo sapiens(human)
Product paraplegin isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006721264.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG 
CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG 
CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG 
AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT 
TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG 
CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC 
AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC 
GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC 
AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG 
ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG 
ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG 
GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA 
GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG 
GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG 
GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG 
GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC 
ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG 
GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT 
TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC 
ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG 
TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC 
ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG 
CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGGGACTGC CAAAAAGAGC 
AAGATCCTGT CCAAGGAAGA ACAGAAAGTG GTTGCGTTTC ATGAGTCGGG CCACGCCTTG 
GTGGGCTGGA TGCTGGAGCA CACGGAGGCC GTGATGAAGG TCTCCATAAC CCCTCGGACA 
AACGCCGCCC TGGGCTTTGC TCAGATGCTC CCCAGAGACC AGCACCTCTT CACCAAGGAG 
CAGCTGTTTG AGCGGATGTG CATGGCCCTG GGAGGACGGG CCTCGGAAGC ACTGTCCTTC 
AACGAGGTCA CTTCTGGGGC ACAGGACGAC CTGAGGAAGG TCACCCGCAT CGCCTACTCC 
ATGGTGAAGC AGTTTGGGAT GGCACCTGGC ATCGGGCCCA TCTCCTTCCC TGAGGCGCAG 
GAGGGCCTCA TGGGCATCGG GCGGCGCCCC TTCAGCCAAG GCCTGCAGCA GATGATGGAC 
CATGAAGCAA GACTGCTGGT GGCCAAGGCC TACAGACACA CCGAGAAGGT GCTGCAGGAC 
AACCTGGACA AGTTGCAGGC GCGAGCACTG TGTGTCACGT GGGGATCGTG TTTGGGGCCC 
TTAGACTGTC GTCCCCAGCC AGCAGCAGCC GCCTCTGGGC AGGTGTGGGC GGTGCCGTCC 
CCGGCTGAAG CAGGCCTGCC TGTGGACCCA TGCTCATCAC GGTTGACAAA GCAGCTCTGC 
AGAGCTCGGG CACATCACAG TGGGAGCCAC AACGGGAGCA AAGCTGCAGC TGGCAAACGC 
CCTTCTGGAA AAGGAAGTGA TAAACTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006721327.1
CDS40..2469
Translation

Target ORF information:

RefSeq Version XM_006721264.4
Organism Homo sapiens(human)
Definition Homo sapiens SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006721264.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG 
CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG 
CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG 
AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT 
TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG 
CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC 
AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC 
GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC 
AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG 
ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG 
ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG 
GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA 
GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG 
GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG 
GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG 
GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC 
ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG 
GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT 
TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC 
ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG 
TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC 
ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG 
CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGGGACTGC CAAAAAGAGC 
AAGATCCTGT CCAAGGAAGA ACAGAAAGTG GTTGCGTTTC ATGAGTCGGG CCACGCCTTG 
GTGGGCTGGA TGCTGGAGCA CACGGAGGCC GTGATGAAGG TCTCCATAAC CCCTCGGACA 
AACGCCGCCC TGGGCTTTGC TCAGATGCTC CCCAGAGACC AGCACCTCTT CACCAAGGAG 
CAGCTGTTTG AGCGGATGTG CATGGCCCTG GGAGGACGGG CCTCGGAAGC ACTGTCCTTC 
AACGAGGTCA CTTCTGGGGC ACAGGACGAC CTGAGGAAGG TCACCCGCAT CGCCTACTCC 
ATGGTGAAGC AGTTTGGGAT GGCACCTGGC ATCGGGCCCA TCTCCTTCCC TGAGGCGCAG 
GAGGGCCTCA TGGGCATCGG GCGGCGCCCC TTCAGCCAAG GCCTGCAGCA GATGATGGAC 
CATGAAGCAA GACTGCTGGT GGCCAAGGCC TACAGACACA CCGAGAAGGT GCTGCAGGAC 
AACCTGGACA AGTTGCAGGC GCGAGCACTG TGTGTCACGT GGGGATCGTG TTTGGGGCCC 
TTAGACTGTC GTCCCCAGCC AGCAGCAGCC GCCTCTGGGC AGGTGTGGGC GGTGCCGTCC 
CCGGCTGAAG CAGGCCTGCC TGTGGACCCA TGCTCATCAC GGTTGACAAA GCAGCTCTGC 
AGAGCTCGGG CACATCACAG TGGGAGCCAC AACGGGAGCA AAGCTGCAGC TGGCAAACGC 
CCTTCTGGAA AAGGAAGTGA TAAACTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu49485
Clone ID Related Accession (Same CDS sequence) XM_005256321.4
Accession Version XM_005256321.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1392bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product paraplegin isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005256321.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG 
CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG 
CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG 
AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT 
TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG 
CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC 
AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC 
GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC 
AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG 
ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG 
ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG 
GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA 
GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG 
GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG 
GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG 
GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC 
ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG 
GATGTGTTCT ACGCAGAAAT AGAAGAGCTG AAACTGGTGT ATCCTTATCA CGGCGTTCTA 
CACAGCGACT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005256378.1
CDS40..1431
Translation

Target ORF information:

RefSeq Version XM_005256321.4
Organism Homo sapiens(human)
Definition Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005256321.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG 
CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG 
CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG 
AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT 
TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG 
CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC 
AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC 
GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC 
AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG 
ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG 
ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG 
GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA 
GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG 
GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG 
GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG 
GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC 
ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG 
GATGTGTTCT ACGCAGAAAT AGAAGAGCTG AAACTGGTGT ATCCTTATCA CGGCGTTCTA 
CACAGCGACT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86234
Clone ID Related Accession (Same CDS sequence) XM_017023597.1
Accession Version XM_017023597.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1857bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product paraplegin isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG 
CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG 
CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG 
AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT 
TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG 
CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC 
AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC 
GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC 
AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG 
ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG 
ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG 
GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA 
GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG 
GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG 
GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG 
GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC 
ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG 
GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT 
TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC 
ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG 
TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC 
ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG 
CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGCCTGGGT GTGGTGGGCT 
CAGCCTAGCC CCAGCCTCAT GGGGGCTGAG CTCCAGCCGC TGACTGTGGG CCGGAGGGCT 
GCTGCCGCCC GTGGCTGTGG AGGGACAGCC GTGCTTTCAG CGCAGCAGGG GCTCTGCGGA 
GAGGTGTGGA TCTGCAGCAC CACCTCTGGT GAAACTCATG GTCCCGACCG TCAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016879086.1
CDS40..1896
Translation

Target ORF information:

RefSeq Version XM_017023597.1
Organism Homo sapiens(human)
Definition Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017023597.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG 
CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG 
CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG 
AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT 
TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG 
CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC 
AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC 
GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC 
AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG 
ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG 
ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG 
GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA 
GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG 
GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG 
GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG 
GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC 
ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG 
GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT 
TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC 
ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG 
TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC 
ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG 
CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGCCTGGGT GTGGTGGGCT 
CAGCCTAGCC CCAGCCTCAT GGGGGCTGAG CTCCAGCCGC TGACTGTGGG CCGGAGGGCT 
GCTGCCGCCC GTGGCTGTGG AGGGACAGCC GTGCTTTCAG CGCAGCAGGG GCTCTGCGGA 
GAGGTGTGGA TCTGCAGCAC CACCTCTGGT GAAACTCATG GTCCCGACCG TCAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86235
Clone ID Related Accession (Same CDS sequence) XM_017023598.1
Accession Version XM_017023598.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1686bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product paraplegin isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG 
CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG 
CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG 
AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT 
TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG 
CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC 
AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC 
GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC 
AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG 
ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG 
ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG 
GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA 
GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG 
GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG 
GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG 
GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC 
ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG 
GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT 
TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC 
ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG 
TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC 
ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG 
CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGTGTGGGG TCTGCGACGT 
CTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016879087.1
CDS40..1725
Translation

Target ORF information:

RefSeq Version XM_017023598.1
Organism Homo sapiens(human)
Definition Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017023598.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG 
CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG 
CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG 
AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT 
TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG 
CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC 
AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC 
GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC 
AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG 
ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG 
ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG 
GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA 
GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG 
GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG 
GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG 
GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC 
ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG 
GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT 
TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC 
ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG 
TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC 
ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG 
CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGTGTGGGG TCTGCGACGT 
CTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed