SPG7 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following SPG7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPG7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu23601 NM_003119.4
Latest version!
Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu23719 NM_199367.3
Latest version!
Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $286.30
$409.00
OHu49482 NM_001363850.1
Latest version!
Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu23719 XM_017023599.1
Latest version!
Homo sapiens SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $286.30
$409.00
OHu49482 XM_006721264.4
Latest version!
Homo sapiens SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu49485 XM_005256321.4
Latest version!
Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
OHu86234 XM_017023597.1
Latest version!
Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu86235 XM_017023598.1
Latest version!
Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
View Old Accession Versions >>
OHu23719 NM_199367.2 Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $286.30
$409.00
OHu23601 NM_003119.3 Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $265.30
$379.00
OHu49482 XM_006721264.3 Homo sapiens SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu23601
    Clone ID Related Accession (Same CDS sequence) NM_003119.4 , NM_003119.3
    Accession Version NM_003119.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2388bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product paraplegin isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY006239.1, Y16610.1 and BC036104.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_003119.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036104.1, SRR1660809.210110.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT
    TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC
    ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG
    TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC
    ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG
    CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGGGACTGC CAAAAAGAGC
    AAGATCCTGT CCAAGGAAGA ACAGAAAGTG GTTGCGTTTC ATGAGTCGGG CCACGCCTTG
    GTGGGCTGGA TGCTGGAGCA CACGGAGGCC GTGATGAAGG TCTCCATAAC CCCTCGGACA
    AACGCCGCCC TGGGCTTTGC TCAGATGCTC CCCAGAGACC AGCACCTCTT CACCAAGGAG
    CAGCTGTTTG AGCGGATGTG CATGGCCCTG GGAGGACGGG CCTCGGAAGC ACTGTCCTTC
    AACGAGGTCA CTTCTGGGGC ACAGGACGAC CTGAGGAAGG TCACCCGCAT CGCCTACTCC
    ATGGTGAAGC AGTTTGGGAT GGCACCTGGC ATCGGGCCCA TCTCCTTCCC TGAGGCGCAG
    GAGGGCCTCA TGGGCATCGG GCGGCGCCCC TTCAGCCAAG GCCTGCAGCA GATGATGGAC
    CATGAAGCAA GACTGCTGGT GGCCAAGGCC TACAGACACA CCGAGAAGGT GCTGCAGGAC
    AACCTGGACA AGTTGCAGGC GCTGGCAAAC GCCCTTCTGG AAAAGGAAGT GATAAACTAT
    GAGGACATTG AGGCTCTCAT TGGCCCGCCG CCCCATGGGC CGAAGAAAAT GATCGCACCG
    CAGAGGTGGA TCGACGCCCA GAGGGAGAAA CAGGACTTGG GCGAGGAGGA GACCGAAGAG
    ACCCAGCAGC CTCCACTTGG AGGCGAAGAG CCGACTTGGC CCAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003110.1
    CDS16..2403
    Translation

    Target ORF information:

    RefSeq Version NM_003119.4
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003119.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT
    TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC
    ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG
    TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC
    ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG
    CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGGGACTGC CAAAAAGAGC
    AAGATCCTGT CCAAGGAAGA ACAGAAAGTG GTTGCGTTTC ATGAGTCGGG CCACGCCTTG
    GTGGGCTGGA TGCTGGAGCA CACGGAGGCC GTGATGAAGG TCTCCATAAC CCCTCGGACA
    AACGCCGCCC TGGGCTTTGC TCAGATGCTC CCCAGAGACC AGCACCTCTT CACCAAGGAG
    CAGCTGTTTG AGCGGATGTG CATGGCCCTG GGAGGACGGG CCTCGGAAGC ACTGTCCTTC
    AACGAGGTCA CTTCTGGGGC ACAGGACGAC CTGAGGAAGG TCACCCGCAT CGCCTACTCC
    ATGGTGAAGC AGTTTGGGAT GGCACCTGGC ATCGGGCCCA TCTCCTTCCC TGAGGCGCAG
    GAGGGCCTCA TGGGCATCGG GCGGCGCCCC TTCAGCCAAG GCCTGCAGCA GATGATGGAC
    CATGAAGCAA GACTGCTGGT GGCCAAGGCC TACAGACACA CCGAGAAGGT GCTGCAGGAC
    AACCTGGACA AGTTGCAGGC GCTGGCAAAC GCCCTTCTGG AAAAGGAAGT GATAAACTAT
    GAGGACATTG AGGCTCTCAT TGGCCCGCCG CCCCATGGGC CGAAGAAAAT GATCGCACCG
    CAGAGGTGGA TCGACGCCCA GAGGGAGAAA CAGGACTTGG GCGAGGAGGA GACCGAAGAG
    ACCCAGCAGC CTCCACTTGG AGGCGAAGAG CCGACTTGGC CCAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23601
    Clone ID Related Accession (Same CDS sequence) NM_003119.4 , NM_003119.3
    Accession Version NM_003119.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2388bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product paraplegin isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY006239.1, Y16610.1, BC036104.1 and AW250773.1. This sequence is a reference standard in the RefSeqGene project. On Mar 12, 2014 this sequence version replaced NM_003119.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.193878.1, BC036104.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT
    TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC
    ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG
    TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC
    ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG
    CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGGGACTGC CAAAAAGAGC
    AAGATCCTGT CCAAGGAAGA ACAGAAAGTG GTTGCGTTTC ATGAGTCGGG CCACGCCTTG
    GTGGGCTGGA TGCTGGAGCA CACGGAGGCC GTGATGAAGG TCTCCATAAC CCCTCGGACA
    AACGCCGCCC TGGGCTTTGC TCAGATGCTC CCCAGAGACC AGCACCTCTT CACCAAGGAG
    CAGCTGTTTG AGCGGATGTG CATGGCCCTG GGAGGACGGG CCTCGGAAGC ACTGTCCTTC
    AACGAGGTCA CTTCTGGGGC ACAGGACGAC CTGAGGAAGG TCACCCGCAT CGCCTACTCC
    ATGGTGAAGC AGTTTGGGAT GGCACCTGGC ATCGGGCCCA TCTCCTTCCC TGAGGCGCAG
    GAGGGCCTCA TGGGCATCGG GCGGCGCCCC TTCAGCCAAG GCCTGCAGCA GATGATGGAC
    CATGAAGCAA GACTGCTGGT GGCCAAGGCC TACAGACACA CCGAGAAGGT GCTGCAGGAC
    AACCTGGACA AGTTGCAGGC GCTGGCAAAC GCCCTTCTGG AAAAGGAAGT GATAAACTAT
    GAGGACATTG AGGCTCTCAT TGGCCCGCCG CCCCATGGGC CGAAGAAAAT GATCGCACCG
    CAGAGGTGGA TCGACGCCCA GAGGGAGAAA CAGGACTTGG GCGAGGAGGA GACCGAAGAG
    ACCCAGCAGC CTCCACTTGG AGGCGAAGAG CCGACTTGGC CCAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003110.1
    CDS31..2418
    Translation

    Target ORF information:

    RefSeq Version NM_003119.3
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003119.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT
    TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC
    ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG
    TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC
    ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG
    CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGGGACTGC CAAAAAGAGC
    AAGATCCTGT CCAAGGAAGA ACAGAAAGTG GTTGCGTTTC ATGAGTCGGG CCACGCCTTG
    GTGGGCTGGA TGCTGGAGCA CACGGAGGCC GTGATGAAGG TCTCCATAAC CCCTCGGACA
    AACGCCGCCC TGGGCTTTGC TCAGATGCTC CCCAGAGACC AGCACCTCTT CACCAAGGAG
    CAGCTGTTTG AGCGGATGTG CATGGCCCTG GGAGGACGGG CCTCGGAAGC ACTGTCCTTC
    AACGAGGTCA CTTCTGGGGC ACAGGACGAC CTGAGGAAGG TCACCCGCAT CGCCTACTCC
    ATGGTGAAGC AGTTTGGGAT GGCACCTGGC ATCGGGCCCA TCTCCTTCCC TGAGGCGCAG
    GAGGGCCTCA TGGGCATCGG GCGGCGCCCC TTCAGCCAAG GCCTGCAGCA GATGATGGAC
    CATGAAGCAA GACTGCTGGT GGCCAAGGCC TACAGACACA CCGAGAAGGT GCTGCAGGAC
    AACCTGGACA AGTTGCAGGC GCTGGCAAAC GCCCTTCTGG AAAAGGAAGT GATAAACTAT
    GAGGACATTG AGGCTCTCAT TGGCCCGCCG CCCCATGGGC CGAAGAAAAT GATCGCACCG
    CAGAGGTGGA TCGACGCCCA GAGGGAGAAA CAGGACTTGG GCGAGGAGGA GACCGAAGAG
    ACCCAGCAGC CTCCACTTGG AGGCGAAGAG CCGACTTGGC CCAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23719
    Clone ID Related Accession (Same CDS sequence) NM_199367.3 , NM_199367.2 , XM_017023599.1
    Accession Version NM_199367.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product paraplegin isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY006239.1, Y16610.1, BC007692.1 and CA447374.1. On Jun 2, 2019 this sequence version replaced NM_199367.2. Transcript Variant: This variant (2) lacks multiple 3' coding exons and contains an alternate 3' terminal exon, resulting in a different 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) contains a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007692.1, SRR1660803.205602.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGTGCCT CTCTTGACCA GCTACCCTCC CAGGGGACCA TGAGGAAGCT CAGAGGAAAG
    ACCCCCGCCT GCTCCTGTCT CACGGAGCCC ACAGGGTCAC GGAGGGCAAT GGAGGGTCAT
    TCGCTCTGCT GGGGTTGCCT TTTGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_955399.1
    CDS16..1485
    Translation

    Target ORF information:

    RefSeq Version NM_199367.3
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_199367.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGTGCCT CTCTTGACCA GCTACCCTCC CAGGGGACCA TGAGGAAGCT CAGAGGAAAG
    ACCCCCGCCT GCTCCTGTCT CACGGAGCCC ACAGGGTCAC GGAGGGCAAT GGAGGGTCAT
    TCGCTCTGCT GGGGTTGCCT TTTGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23719
    Clone ID Related Accession (Same CDS sequence) NM_199367.3 , NM_199367.2 , XM_017023599.1
    Accession Version NM_199367.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product paraplegin isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY006239.1, Y16610.1, BC007692.1 and CA447374.1. On Mar 12, 2014 this sequence version replaced NM_199367.1. Transcript Variant: This variant (2) lacks multiple 3' coding exons and contains an alternate 3' terminal exon, resulting in a different 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) contains a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007692.1, SRR1163658.93287.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGTGCCT CTCTTGACCA GCTACCCTCC CAGGGGACCA TGAGGAAGCT CAGAGGAAAG
    ACCCCCGCCT GCTCCTGTCT CACGGAGCCC ACAGGGTCAC GGAGGGCAAT GGAGGGTCAT
    TCGCTCTGCT GGGGTTGCCT TTTGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_955399.1
    CDS31..1500
    Translation

    Target ORF information:

    RefSeq Version NM_199367.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_199367.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGTGCCT CTCTTGACCA GCTACCCTCC CAGGGGACCA TGAGGAAGCT CAGAGGAAAG
    ACCCCCGCCT GCTCCTGTCT CACGGAGCCC ACAGGGTCAC GGAGGGCAAT GGAGGGTCAT
    TCGCTCTGCT GGGGTTGCCT TTTGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu49482
    Clone ID Related Accession (Same CDS sequence) NM_001363850.1 , XM_006721264.3 , XM_006721264.4
    Accession Version NM_001363850.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product paraplegin isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY006239.1, Y16610.1, BC036104.1, AC092123.4 and BC052587.1. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT
    TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC
    ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG
    TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC
    ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG
    CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGGGACTGC CAAAAAGAGC
    AAGATCCTGT CCAAGGAAGA ACAGAAAGTG GTTGCGTTTC ATGAGTCGGG CCACGCCTTG
    GTGGGCTGGA TGCTGGAGCA CACGGAGGCC GTGATGAAGG TCTCCATAAC CCCTCGGACA
    AACGCCGCCC TGGGCTTTGC TCAGATGCTC CCCAGAGACC AGCACCTCTT CACCAAGGAG
    CAGCTGTTTG AGCGGATGTG CATGGCCCTG GGAGGACGGG CCTCGGAAGC ACTGTCCTTC
    AACGAGGTCA CTTCTGGGGC ACAGGACGAC CTGAGGAAGG TCACCCGCAT CGCCTACTCC
    ATGGTGAAGC AGTTTGGGAT GGCACCTGGC ATCGGGCCCA TCTCCTTCCC TGAGGCGCAG
    GAGGGCCTCA TGGGCATCGG GCGGCGCCCC TTCAGCCAAG GCCTGCAGCA GATGATGGAC
    CATGAAGCAA GACTGCTGGT GGCCAAGGCC TACAGACACA CCGAGAAGGT GCTGCAGGAC
    AACCTGGACA AGTTGCAGGC GCGAGCACTG TGTGTCACGT GGGGATCGTG TTTGGGGCCC
    TTAGACTGTC GTCCCCAGCC AGCAGCAGCC GCCTCTGGGC AGGTGTGGGC GGTGCCGTCC
    CCGGCTGAAG CAGGCCTGCC TGTGGACCCA TGCTCATCAC GGTTGACAAA GCAGCTCTGC
    AGAGCTCGGG CACATCACAG TGGGAGCCAC AACGGGAGCA AAGCTGCAGC TGGCAAACGC
    CCTTCTGGAA AAGGAAGTGA TAAACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001350779.1
    CDS31..2460
    Translation

    Target ORF information:

    RefSeq Version NM_001363850.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001363850.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT
    TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC
    ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG
    TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC
    ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG
    CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGGGACTGC CAAAAAGAGC
    AAGATCCTGT CCAAGGAAGA ACAGAAAGTG GTTGCGTTTC ATGAGTCGGG CCACGCCTTG
    GTGGGCTGGA TGCTGGAGCA CACGGAGGCC GTGATGAAGG TCTCCATAAC CCCTCGGACA
    AACGCCGCCC TGGGCTTTGC TCAGATGCTC CCCAGAGACC AGCACCTCTT CACCAAGGAG
    CAGCTGTTTG AGCGGATGTG CATGGCCCTG GGAGGACGGG CCTCGGAAGC ACTGTCCTTC
    AACGAGGTCA CTTCTGGGGC ACAGGACGAC CTGAGGAAGG TCACCCGCAT CGCCTACTCC
    ATGGTGAAGC AGTTTGGGAT GGCACCTGGC ATCGGGCCCA TCTCCTTCCC TGAGGCGCAG
    GAGGGCCTCA TGGGCATCGG GCGGCGCCCC TTCAGCCAAG GCCTGCAGCA GATGATGGAC
    CATGAAGCAA GACTGCTGGT GGCCAAGGCC TACAGACACA CCGAGAAGGT GCTGCAGGAC
    AACCTGGACA AGTTGCAGGC GCGAGCACTG TGTGTCACGT GGGGATCGTG TTTGGGGCCC
    TTAGACTGTC GTCCCCAGCC AGCAGCAGCC GCCTCTGGGC AGGTGTGGGC GGTGCCGTCC
    CCGGCTGAAG CAGGCCTGCC TGTGGACCCA TGCTCATCAC GGTTGACAAA GCAGCTCTGC
    AGAGCTCGGG CACATCACAG TGGGAGCCAC AACGGGAGCA AAGCTGCAGC TGGCAAACGC
    CCTTCTGGAA AAGGAAGTGA TAAACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23719
    Clone ID Related Accession (Same CDS sequence) NM_199367.3 , NM_199367.2 , XM_017023599.1
    Accession Version XM_017023599.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product paraplegin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGTGCCT CTCTTGACCA GCTACCCTCC CAGGGGACCA TGAGGAAGCT CAGAGGAAAG
    ACCCCCGCCT GCTCCTGTCT CACGGAGCCC ACAGGGTCAC GGAGGGCAAT GGAGGGTCAT
    TCGCTCTGCT GGGGTTGCCT TTTGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016879088.1
    CDS40..1509
    Translation

    Target ORF information:

    RefSeq Version XM_017023599.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017023599.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGTGCCT CTCTTGACCA GCTACCCTCC CAGGGGACCA TGAGGAAGCT CAGAGGAAAG
    ACCCCCGCCT GCTCCTGTCT CACGGAGCCC ACAGGGTCAC GGAGGGCAAT GGAGGGTCAT
    TCGCTCTGCT GGGGTTGCCT TTTGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu49482
    Clone ID Related Accession (Same CDS sequence) NM_001363850.1 , XM_006721264.3 , XM_006721264.4
    Accession Version XM_006721264.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product paraplegin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006721264.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT
    TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC
    ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG
    TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC
    ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG
    CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGGGACTGC CAAAAAGAGC
    AAGATCCTGT CCAAGGAAGA ACAGAAAGTG GTTGCGTTTC ATGAGTCGGG CCACGCCTTG
    GTGGGCTGGA TGCTGGAGCA CACGGAGGCC GTGATGAAGG TCTCCATAAC CCCTCGGACA
    AACGCCGCCC TGGGCTTTGC TCAGATGCTC CCCAGAGACC AGCACCTCTT CACCAAGGAG
    CAGCTGTTTG AGCGGATGTG CATGGCCCTG GGAGGACGGG CCTCGGAAGC ACTGTCCTTC
    AACGAGGTCA CTTCTGGGGC ACAGGACGAC CTGAGGAAGG TCACCCGCAT CGCCTACTCC
    ATGGTGAAGC AGTTTGGGAT GGCACCTGGC ATCGGGCCCA TCTCCTTCCC TGAGGCGCAG
    GAGGGCCTCA TGGGCATCGG GCGGCGCCCC TTCAGCCAAG GCCTGCAGCA GATGATGGAC
    CATGAAGCAA GACTGCTGGT GGCCAAGGCC TACAGACACA CCGAGAAGGT GCTGCAGGAC
    AACCTGGACA AGTTGCAGGC GCGAGCACTG TGTGTCACGT GGGGATCGTG TTTGGGGCCC
    TTAGACTGTC GTCCCCAGCC AGCAGCAGCC GCCTCTGGGC AGGTGTGGGC GGTGCCGTCC
    CCGGCTGAAG CAGGCCTGCC TGTGGACCCA TGCTCATCAC GGTTGACAAA GCAGCTCTGC
    AGAGCTCGGG CACATCACAG TGGGAGCCAC AACGGGAGCA AAGCTGCAGC TGGCAAACGC
    CCTTCTGGAA AAGGAAGTGA TAAACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006721327.1
    CDS40..2469
    Translation

    Target ORF information:

    RefSeq Version XM_006721264.3
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006721264.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT
    TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC
    ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG
    TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC
    ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG
    CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGGGACTGC CAAAAAGAGC
    AAGATCCTGT CCAAGGAAGA ACAGAAAGTG GTTGCGTTTC ATGAGTCGGG CCACGCCTTG
    GTGGGCTGGA TGCTGGAGCA CACGGAGGCC GTGATGAAGG TCTCCATAAC CCCTCGGACA
    AACGCCGCCC TGGGCTTTGC TCAGATGCTC CCCAGAGACC AGCACCTCTT CACCAAGGAG
    CAGCTGTTTG AGCGGATGTG CATGGCCCTG GGAGGACGGG CCTCGGAAGC ACTGTCCTTC
    AACGAGGTCA CTTCTGGGGC ACAGGACGAC CTGAGGAAGG TCACCCGCAT CGCCTACTCC
    ATGGTGAAGC AGTTTGGGAT GGCACCTGGC ATCGGGCCCA TCTCCTTCCC TGAGGCGCAG
    GAGGGCCTCA TGGGCATCGG GCGGCGCCCC TTCAGCCAAG GCCTGCAGCA GATGATGGAC
    CATGAAGCAA GACTGCTGGT GGCCAAGGCC TACAGACACA CCGAGAAGGT GCTGCAGGAC
    AACCTGGACA AGTTGCAGGC GCGAGCACTG TGTGTCACGT GGGGATCGTG TTTGGGGCCC
    TTAGACTGTC GTCCCCAGCC AGCAGCAGCC GCCTCTGGGC AGGTGTGGGC GGTGCCGTCC
    CCGGCTGAAG CAGGCCTGCC TGTGGACCCA TGCTCATCAC GGTTGACAAA GCAGCTCTGC
    AGAGCTCGGG CACATCACAG TGGGAGCCAC AACGGGAGCA AAGCTGCAGC TGGCAAACGC
    CCTTCTGGAA AAGGAAGTGA TAAACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu49482
    Clone ID Related Accession (Same CDS sequence) NM_001363850.1 , XM_006721264.3 , XM_006721264.4
    Accession Version XM_006721264.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product paraplegin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006721264.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT
    TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC
    ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG
    TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC
    ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG
    CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGGGACTGC CAAAAAGAGC
    AAGATCCTGT CCAAGGAAGA ACAGAAAGTG GTTGCGTTTC ATGAGTCGGG CCACGCCTTG
    GTGGGCTGGA TGCTGGAGCA CACGGAGGCC GTGATGAAGG TCTCCATAAC CCCTCGGACA
    AACGCCGCCC TGGGCTTTGC TCAGATGCTC CCCAGAGACC AGCACCTCTT CACCAAGGAG
    CAGCTGTTTG AGCGGATGTG CATGGCCCTG GGAGGACGGG CCTCGGAAGC ACTGTCCTTC
    AACGAGGTCA CTTCTGGGGC ACAGGACGAC CTGAGGAAGG TCACCCGCAT CGCCTACTCC
    ATGGTGAAGC AGTTTGGGAT GGCACCTGGC ATCGGGCCCA TCTCCTTCCC TGAGGCGCAG
    GAGGGCCTCA TGGGCATCGG GCGGCGCCCC TTCAGCCAAG GCCTGCAGCA GATGATGGAC
    CATGAAGCAA GACTGCTGGT GGCCAAGGCC TACAGACACA CCGAGAAGGT GCTGCAGGAC
    AACCTGGACA AGTTGCAGGC GCGAGCACTG TGTGTCACGT GGGGATCGTG TTTGGGGCCC
    TTAGACTGTC GTCCCCAGCC AGCAGCAGCC GCCTCTGGGC AGGTGTGGGC GGTGCCGTCC
    CCGGCTGAAG CAGGCCTGCC TGTGGACCCA TGCTCATCAC GGTTGACAAA GCAGCTCTGC
    AGAGCTCGGG CACATCACAG TGGGAGCCAC AACGGGAGCA AAGCTGCAGC TGGCAAACGC
    CCTTCTGGAA AAGGAAGTGA TAAACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006721327.1
    CDS40..2469
    Translation

    Target ORF information:

    RefSeq Version XM_006721264.4
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006721264.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT
    TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC
    ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG
    TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC
    ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG
    CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGGGACTGC CAAAAAGAGC
    AAGATCCTGT CCAAGGAAGA ACAGAAAGTG GTTGCGTTTC ATGAGTCGGG CCACGCCTTG
    GTGGGCTGGA TGCTGGAGCA CACGGAGGCC GTGATGAAGG TCTCCATAAC CCCTCGGACA
    AACGCCGCCC TGGGCTTTGC TCAGATGCTC CCCAGAGACC AGCACCTCTT CACCAAGGAG
    CAGCTGTTTG AGCGGATGTG CATGGCCCTG GGAGGACGGG CCTCGGAAGC ACTGTCCTTC
    AACGAGGTCA CTTCTGGGGC ACAGGACGAC CTGAGGAAGG TCACCCGCAT CGCCTACTCC
    ATGGTGAAGC AGTTTGGGAT GGCACCTGGC ATCGGGCCCA TCTCCTTCCC TGAGGCGCAG
    GAGGGCCTCA TGGGCATCGG GCGGCGCCCC TTCAGCCAAG GCCTGCAGCA GATGATGGAC
    CATGAAGCAA GACTGCTGGT GGCCAAGGCC TACAGACACA CCGAGAAGGT GCTGCAGGAC
    AACCTGGACA AGTTGCAGGC GCGAGCACTG TGTGTCACGT GGGGATCGTG TTTGGGGCCC
    TTAGACTGTC GTCCCCAGCC AGCAGCAGCC GCCTCTGGGC AGGTGTGGGC GGTGCCGTCC
    CCGGCTGAAG CAGGCCTGCC TGTGGACCCA TGCTCATCAC GGTTGACAAA GCAGCTCTGC
    AGAGCTCGGG CACATCACAG TGGGAGCCAC AACGGGAGCA AAGCTGCAGC TGGCAAACGC
    CCTTCTGGAA AAGGAAGTGA TAAACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu49485
    Clone ID Related Accession (Same CDS sequence) XM_005256321.4
    Accession Version XM_005256321.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product paraplegin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005256321.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGTGTTCT ACGCAGAAAT AGAAGAGCTG AAACTGGTGT ATCCTTATCA CGGCGTTCTA
    CACAGCGACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005256378.1
    CDS40..1431
    Translation

    Target ORF information:

    RefSeq Version XM_005256321.4
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005256321.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGTGTTCT ACGCAGAAAT AGAAGAGCTG AAACTGGTGT ATCCTTATCA CGGCGTTCTA
    CACAGCGACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86234
    Clone ID Related Accession (Same CDS sequence) XM_017023597.1
    Accession Version XM_017023597.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1857bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product paraplegin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT
    TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC
    ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG
    TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC
    ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG
    CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGCCTGGGT GTGGTGGGCT
    CAGCCTAGCC CCAGCCTCAT GGGGGCTGAG CTCCAGCCGC TGACTGTGGG CCGGAGGGCT
    GCTGCCGCCC GTGGCTGTGG AGGGACAGCC GTGCTTTCAG CGCAGCAGGG GCTCTGCGGA
    GAGGTGTGGA TCTGCAGCAC CACCTCTGGT GAAACTCATG GTCCCGACCG TCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016879086.1
    CDS40..1896
    Translation

    Target ORF information:

    RefSeq Version XM_017023597.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017023597.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT
    TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC
    ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG
    TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC
    ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG
    CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGCCTGGGT GTGGTGGGCT
    CAGCCTAGCC CCAGCCTCAT GGGGGCTGAG CTCCAGCCGC TGACTGTGGG CCGGAGGGCT
    GCTGCCGCCC GTGGCTGTGG AGGGACAGCC GTGCTTTCAG CGCAGCAGGG GCTCTGCGGA
    GAGGTGTGGA TCTGCAGCAC CACCTCTGGT GAAACTCATG GTCCCGACCG TCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86235
    Clone ID Related Accession (Same CDS sequence) XM_017023598.1
    Accession Version XM_017023598.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product paraplegin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT
    TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC
    ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG
    TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC
    ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG
    CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGTGTGGGG TCTGCGACGT
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016879087.1
    CDS40..1725
    Translation

    Target ORF information:

    RefSeq Version XM_017023598.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017023598.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCCGTGC TGCTGCTGCT GCTCCGTGCC CTCCGCCGGG GTCCAGGCCC GGGTCCTCGG 
    CCGCTGTGGG GCCCAGGCCC GGCCTGGAGT CCAGGGTTCC CCGCCAGGCC CGGGAGGGGG
    CGGCCGTACA TGGCCAGCAG GCCTCCGGGG GACCTCGCCG AGGCTGGAGG CCGAGCTCTG
    CAGAGCTTAC AATTGAGACT GCTAACCCCT ACCTTTGAAG GGATCAACGG ATTGTTGTTG
    AAACAACATT TAGTTCAGAA TCCAGTCAGA CTCTGGCAAC TTTTAGGTGG TACTTTCTAT
    TTTAACACCT CAAGGTTGAA GCAGAAGAAT AAGGAGAAGG ATAAGTCGAA GGGGAAGGCG
    CCTGAAGAGG ACGAAGAGGA GAGGAGACGC CGTGAGCGGG ACGACCAGAT GTACCGAGAG
    CGGCTGCGCA CCTTGCTGGT CATCGCGGTT GTCATGAGCC TCCTGAATGC TCTCAGCACC
    AGCGGAGGCA GCATTTCCTG GAACGACTTT GTCCACGAGA TGCTGGCCAA GGGCGAGGTG
    CAGCGCGTCC AGGTGGTGCC TGAGAGCGAC GTGGTGGAAG TCTACCTGCA CCCTGGAGCC
    GTGGTGTTTG GGCGGCCTCG GCTAGCCTTG ATGTACCGAA TGCAGGTTGC AAATATTGAC
    AAGTTTGAAG AGAAGCTTCG AGCAGCTGAA GATGAGCTGA ATATCGAGGC CAAGGACAGG
    ATCCCAGTTT CCTACAAGCG AACAGGATTC TTTGGAAATG CCCTGTACTC TGTGGGGATG
    ACGGCAGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCCGGGAT GACTGGAAGG
    GAAGGTGGAT TCAGTGCTTT TAATCAGCTT AAAATGGCTC GTTTCACCAT TGTGGATGGG
    AAGATGGGGA AAGGAGTCAG CTTCAAAGAC GTGGCAGGAA TGCACGAAGC CAAACTGGAA
    GTCCGCGAGT TTGTGGATTA TCTGAAGAGC CCAGAACGCT TCCTCCAGCT TGGCGCCAAG
    GTCCCAAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGTG GGAAGACGCT GCTGGCCAAG
    GCGGTGGCCA CGGAGGCTCA GGTGCCCTTC CTGGCGATGG CCGGCCCAGA GTTCGTGGAG
    GTCATTGGAG GCCTCGGCGC TGCCCGTGTG CGGAGCCTCT TTAAGGAAGC CCGAGCCCGG
    GCCCCCTGCA TCGTCTACAT CGATGAGATC GACGCGGTGG GCAAGAAGCG CTCCACCACC
    ATGTCCGGCT TCTCCAACAC GGAGGAGGAG CAGACGCTCA ACCAGCTTCT GGTAGAAATG
    GATGGAATGG GTACCACAGA CCATGTCATC GTCCTGGCGT CCACGAACCG AGCTGACATT
    TTGGACGGTG CTCTGATGAG GCCAGGCCGA CTGGACCGGC ACGTCTTCAT TGATCTCCCC
    ACGCTGCAGG AGAGGCGGGA GATTTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACCCAG
    TCCAGCACCT TTTACTCCCA GCGTCTGGCA GAGCTGACAC CAGGATTCAG TGGGGCTGAC
    ATCGCCAACA TCTGCAATGA GGCTGCGCTG CACGCGGCGC GGGAGGGACA CACTTCCGTG
    CACACTCTCA ACTTCGAGTA CGCCGTGGAG CGCGTCCTCG CAGTGTGGGG TCTGCGACGT
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.