Atg9 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atg9
Entrez Gene ID	36821
Full Name	Autophagy-related 9
Synonyms	ATG9,CG3615,Dmel\CG3615,atg9
General protein information	Preferred Names Autophagy-related 9 Names CG3615 gene product from transcript CG3615-RA Atg9-PA Atg9-PB Autophagy-specific gene 9 CG3615-PA CG3615-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53B1-53B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274094.2	NP_001261023.1	Autophagy-related 9, isoform B
NM_137270.4	NP_611114.1	Autophagy-related 9, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Atg9	NP_001261023.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001762	XP_321322.5
Xenopus tropicalis (tropical clawed frog)	LOC100486160	XP_004919888.1
Caenorhabditis elegans (roundworm)	atg-9	NP_872184.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The transmembrane protein Atg9 accumulates on refractory to Sigma P (Ref(2)P) aggregates in Atg7, Atg8a and Atg2 mutants
Title: Different effects of Atg2 and Atg18 mutations on Atg8a and Atg9 trafficking during starvation in Drosophila.

Our findings indicate a dual role for Atg9 in the regulation of JNK signaling and autophagy under oxidative stress conditions.
Title: Atg9 interacts with dTRAF2/TRAF6 to regulate oxidative stress-induced JNK activation and autophagy induction.

Evidence of a link between Atg1 and the control of Atg9-mediated autophagosome formation through the myosin II motor protein.
Title: Atg1-mediated myosin II activation regulates autophagosome formation during starvation-induced autophagy.

The following Atg9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atg9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10209	NM_137270.4 Latest version!	Drosophila melanogaster Autophagy-related 9 (Atg9), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa13269	NM_001274094.2 Latest version!	Drosophila melanogaster Autophagy-related 9 (Atg9), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atg9 ( NM_137270.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611114.1 Drosophila melanogaster Autophagy-related 9 (Atg9), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa10209
Clone ID Related Accession (Same CDS sequence)	NM_137270.4
Accession Version	NM_137270.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 9, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137270.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTAGCC CACATATCAA CTACCGCTCC CTGGCCGAGG AGGCGGCCAG TCCGTTCCTG 
GAGCACCATC CCTCCACAGG CCAAGGCCCA TCCAAAACAC AAGACGCAAA AGCGAACGCA 
GCTGCTGCGC ATTTAGACCC CCTCGGTGAA CACGGGCTGG AGCAGCCGCT GGATGAGCAC 
GACACCGAGC ATGAGGGCGA GGACACGCCT CGAAACAGTG GCGTCATGAT CCACATGGTT 
CCGGAGACGG GGCGTGCGCG GTGGAACCAC ATCGAGGACC TTGACTCGTT CTTCTCGCGA 
ATGTATCAGT ACCAGCAGAA GCACGGATTC ACAGTGATTG TAGTGGACGA AATGCTACAG 
GTTCTGGAGT TTGGTTTCGT CGTCTGGCTA CTTGCCTTTG TGATGCACTG CGTCCGATTT 
GACGTGCTCT TTGGGGACAC CCCTCCAGGG GGACTTAACC CCAACAAGAC AACTTTATCC 
GATGTGATGT ACCCCACAGG CGAGTGCTTA GCCAACTTCA CTTGGGTCAC GTACCTGGTT 
GTGTTCATAG CCGCTATCTA TTTGGGCATA AGGCTTCTGA AAATGGTGTA CCATATTACC 
CAGTACGCCG ATATAAAGAG ATTCTACAAC TCTGCGCTTC ATATCGAGGA TTCGGATCTG 
GACAACTTTA CGTGGCACGA GGTGCAACAG CGGATTCGTC GCGTCCAGGC CGAACAGCAT 
ATGTGCATCG ACAAGGAGTC GCTTACGGAA TTGGACATCT ATCACCGAGT TCTGCGCTTC 
AAGAACTACC TAGTGGCACT GATGAACAAG CAACTGCTGC CTGTAAGGTT CCACATACCG 
CTGTATGGCG AAGTGGTCTC GCTGAGTAGA GGTATGTTGT TCAACATTGA CTTCATCTTG 
TTTCGCGGCC CTGGATCTCC CTTCCAGAAC AATTGGCAGC TGCGTGATGA GTTTGCGGTA 
AGAAGCAATC AAACGGAGCT GGCGCAGCGA CTCTCCAAAC TGATATTGGG GGTGGCGTTG 
TTAAATTTGG TCCTAGCGCC TGTGATCTTC GTATGGCAAC TTATCTACTT CAGTTTCTCC 
TATGCGAACA TTCTTCGTAA GGAGCCTGGT GCCCTTGGCC TGCGTACATG GTCCAATTAC 
GGCCGCCTCT ACCTGCGCCA CTTTAATGAA CTGGACCACG AACTAGATGC CAGGCTGAAT 
CGGGCATATG ACTACGCGGA CCGCTATCTC AACTCCTTTT CCTCGCCACT GGCCGCTGTG 
ATAGCCAAAA ACCTACTCTT TATCAGTGGA GGACTACTAC TTCTAATCCT TGCATTGGGC 
ATTTACGAGG AACACGTCTT CCAGGTGGAG CATTTGCTTG CCATCCTTGC AGGGCTCGGC 
GCCATAGGTG TGGTCTGCAG AACTCTAATA CCGGATGAGA ATCTCGTTTG GTGCCCAGAG 
CAGCTAATGA CCGCCATTCT GGCGCACGTT CACTACCTGC CCTCCGAGTG GCGGCAGCAG 
GCGCACACGA CAAAGGTTCG CCAGGAGTTT AGCAACTTCT TTCAGTTTAA GGCTGGCTAC 
CTACTCAGCG AAATCTTCAG TCCCTTCGTC ACACCCTTCG TGCTGATCTT TGTGTTCAGG 
CCAAAGGCCA TAGAGCTGGT GCGTTTCTTC AGGACGTTCA CCGTATCCGT GCGAGGAGTG 
GGCAACGTAT GCTCCTTTGC CCAGATGGAT GTTCGCAAGC ATGGCAATCC CGACTGGCAG 
TTAACCAGCG AACTGGAGGA GATGACCCGG GCTACAGCTC AACAGCCGCA GCAGGAGCCA 
CAGCAGCAAA GTTTGGCCGG CGGCAAAACG GAGATGTCGC TTCTTCGCTT CACCCTGAAC 
AATCCCGAGT GGCAGATGCC CAAGGAGGCC AAGCAGTTCC TGCGCGGCGT TAGAGAGCAC 
GCCGTTGGTG AACTAGTCCA GGCCAAGACG TCGATGGTGC AGGAGAACCC GCTGACCAAC 
AGCCTCATCT CATTTGGCAC CATGGGAGCG GATTATTGTT CCATTGCGAA CTCTGTGCTG 
ACCGCACAGG TGACGCCCCA GCAACTGGAG ATTTCGCAGT CGTTGCGTCC GGGTCTAGGA 
CCGGTATCTG GAGGATTCCC CGTCGCAGCC AGCGACTTCC GCCAGATGTT GCAGCAGAAT 
CTGTCCGCCA GTGTCGGCCC GCTGGACAGC ATGCGCCGAT TGCGCCTCAG CCGGGCTGAA 
GGCCGCTTGG AGGGTCCGAC GGATACACTG CTCTACGGAC TCTGTGGTGT GGACCCGCGC 
GTGGGCAGCA CTCCCTTGAA TGTGGGCGTG GCCGACATGT GCCTCAGTGC CCTCTATTTG 
CACGAGCTGA ACCAGCAGAA GCGCCAAGCG CGCCAGTCTC GCATCGACGA GGCCGAGGAC 
GAGCGTCCTG GTACGAGCCA TTGGCCACCG CGACCACCAG CTGCTCCGTC GGCTGATACT 
GGCTTCGGCT CCCGCCATAC CGTGATTACC TCAAAGGCTG CCGAGAGCAC GCCGCTTTTG 
GGTAGCATCC GCTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611114.1
CDS	122..2659
Translation	MSSPHINYRSLAEEAASPFLEHHPSTGQGPSKTQDAKANAAAAHLDPLGEHGLEQPLDEHDTEHEGEDTPRNSGVMIHMVPETGRARWNHIEDLDSFFSRMYQYQQKHGFTVIVVDEMLQVLEFGFVVWLLAFVMHCVRFDVLFGDTPPGGLNPNKTTLSDVMYPTGECLANFTWVTYLVVFIAAIYLGIRLLKMVYHITQYADIKRFYNSALHIEDSDLDNFTWHEVQQRIRRVQAEQHMCIDKESLTELDIYHRVLRFKNYLVALMNKQLLPVRFHIPLYGEVVSLSRGMLFNIDFILFRGPGSPFQNNWQLRDEFAVRSNQTELAQRLSKLILGVALLNLVLAPVIFVWQLIYFSFSYANILRKEPGALGLRTWSNYGRLYLRHFNELDHELDARLNRAYDYADRYLNSFSSPLAAVIAKNLLFISGGLLLLILALGIYEEHVFQVEHLLAILAGLGAIGVVCRTLIPDENLVWCPEQLMTAILAHVHYLPSEWRQQAHTTKVRQEFSNFFQFKAGYLLSEIFSPFVTPFVLIFVFRPKAIELVRFFRTFTVSVRGVGNVCSFAQMDVRKHGNPDWQLTSELEEMTRATAQQPQQEPQQQSLAGGKTEMSLLRFTLNNPEWQMPKEAKQFLRGVREHAVGELVQAKTSMVQENPLTNSLISFGTMGADYCSIANSVLTAQVTPQQLEISQSLRPGLGPVSGGFPVAASDFRQMLQQNLSASVGPLDSMRRLRLSRAEGRLEGPTDTLLYGLCGVDPRVGSTPLNVGVADMCLSALYLHELNQQKRQARQSRIDEAEDERPGTSHWPPRPPAAPSADTGFGSRHTVITSKAAESTPLLGSIRS

Target ORF information:

RefSeq Version	NM_137270.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 9 (Atg9), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137270.4

ORF Insert Sequence:

ATGTCTAGCC CACATATCAA CTACCGCTCC CTGGCCGAGG AGGCGGCCAG TCCGTTCCTG 
GAGCACCATC CCTCCACAGG CCAAGGCCCA TCCAAAACAC AAGACGCAAA AGCGAACGCA 
GCTGCTGCGC ATTTAGACCC CCTCGGTGAA CACGGGCTGG AGCAGCCGCT GGATGAGCAC 
GACACCGAGC ATGAGGGCGA GGACACGCCT CGAAACAGTG GCGTCATGAT CCACATGGTT 
CCGGAGACGG GGCGTGCGCG GTGGAACCAC ATCGAGGACC TTGACTCGTT CTTCTCGCGA 
ATGTATCAGT ACCAGCAGAA GCACGGATTC ACAGTGATTG TAGTGGACGA AATGCTACAG 
GTTCTGGAGT TTGGTTTCGT CGTCTGGCTA CTTGCCTTTG TGATGCACTG CGTCCGATTT 
GACGTGCTCT TTGGGGACAC CCCTCCAGGG GGACTTAACC CCAACAAGAC AACTTTATCC 
GATGTGATGT ACCCCACAGG CGAGTGCTTA GCCAACTTCA CTTGGGTCAC GTACCTGGTT 
GTGTTCATAG CCGCTATCTA TTTGGGCATA AGGCTTCTGA AAATGGTGTA CCATATTACC 
CAGTACGCCG ATATAAAGAG ATTCTACAAC TCTGCGCTTC ATATCGAGGA TTCGGATCTG 
GACAACTTTA CGTGGCACGA GGTGCAACAG CGGATTCGTC GCGTCCAGGC CGAACAGCAT 
ATGTGCATCG ACAAGGAGTC GCTTACGGAA TTGGACATCT ATCACCGAGT TCTGCGCTTC 
AAGAACTACC TAGTGGCACT GATGAACAAG CAACTGCTGC CTGTAAGGTT CCACATACCG 
CTGTATGGCG AAGTGGTCTC GCTGAGTAGA GGTATGTTGT TCAACATTGA CTTCATCTTG 
TTTCGCGGCC CTGGATCTCC CTTCCAGAAC AATTGGCAGC TGCGTGATGA GTTTGCGGTA 
AGAAGCAATC AAACGGAGCT GGCGCAGCGA CTCTCCAAAC TGATATTGGG GGTGGCGTTG 
TTAAATTTGG TCCTAGCGCC TGTGATCTTC GTATGGCAAC TTATCTACTT CAGTTTCTCC 
TATGCGAACA TTCTTCGTAA GGAGCCTGGT GCCCTTGGCC TGCGTACATG GTCCAATTAC 
GGCCGCCTCT ACCTGCGCCA CTTTAATGAA CTGGACCACG AACTAGATGC CAGGCTGAAT 
CGGGCATATG ACTACGCGGA CCGCTATCTC AACTCCTTTT CCTCGCCACT GGCCGCTGTG 
ATAGCCAAAA ACCTACTCTT TATCAGTGGA GGACTACTAC TTCTAATCCT TGCATTGGGC 
ATTTACGAGG AACACGTCTT CCAGGTGGAG CATTTGCTTG CCATCCTTGC AGGGCTCGGC 
GCCATAGGTG TGGTCTGCAG AACTCTAATA CCGGATGAGA ATCTCGTTTG GTGCCCAGAG 
CAGCTAATGA CCGCCATTCT GGCGCACGTT CACTACCTGC CCTCCGAGTG GCGGCAGCAG 
GCGCACACGA CAAAGGTTCG CCAGGAGTTT AGCAACTTCT TTCAGTTTAA GGCTGGCTAC 
CTACTCAGCG AAATCTTCAG TCCCTTCGTC ACACCCTTCG TGCTGATCTT TGTGTTCAGG 
CCAAAGGCCA TAGAGCTGGT GCGTTTCTTC AGGACGTTCA CCGTATCCGT GCGAGGAGTG 
GGCAACGTAT GCTCCTTTGC CCAGATGGAT GTTCGCAAGC ATGGCAATCC CGACTGGCAG 
TTAACCAGCG AACTGGAGGA GATGACCCGG GCTACAGCTC AACAGCCGCA GCAGGAGCCA 
CAGCAGCAAA GTTTGGCCGG CGGCAAAACG GAGATGTCGC TTCTTCGCTT CACCCTGAAC 
AATCCCGAGT GGCAGATGCC CAAGGAGGCC AAGCAGTTCC TGCGCGGCGT TAGAGAGCAC 
GCCGTTGGTG AACTAGTCCA GGCCAAGACG TCGATGGTGC AGGAGAACCC GCTGACCAAC 
AGCCTCATCT CATTTGGCAC CATGGGAGCG GATTATTGTT CCATTGCGAA CTCTGTGCTG 
ACCGCACAGG TGACGCCCCA GCAACTGGAG ATTTCGCAGT CGTTGCGTCC GGGTCTAGGA 
CCGGTATCTG GAGGATTCCC CGTCGCAGCC AGCGACTTCC GCCAGATGTT GCAGCAGAAT 
CTGTCCGCCA GTGTCGGCCC GCTGGACAGC ATGCGCCGAT TGCGCCTCAG CCGGGCTGAA 
GGCCGCTTGG AGGGTCCGAC GGATACACTG CTCTACGGAC TCTGTGGTGT GGACCCGCGC 
GTGGGCAGCA CTCCCTTGAA TGTGGGCGTG GCCGACATGT GCCTCAGTGC CCTCTATTTG 
CACGAGCTGA ACCAGCAGAA GCGCCAAGCG CGCCAGTCTC GCATCGACGA GGCCGAGGAC 
GAGCGTCCTG GTACGAGCCA TTGGCCACCG CGACCACCAG CTGCTCCGTC GGCTGATACT 
GGCTTCGGCT CCCGCCATAC CGTGATTACC TCAAAGGCTG CCGAGAGCAC GCCGCTTTTG 
GGTAGCATCC GCTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg9 ( NM_001274094.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261023.1 Drosophila melanogaster Autophagy-related 9 (Atg9), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13269
Clone ID Related Accession (Same CDS sequence)	NM_001274094.2
Accession Version	NM_001274094.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 9, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274094.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTAGCC CACATATCAA CTACCGCTCC CTGGCCGAGG AGGCGGCCAG TCCGTTCCTG 
GAGCACCATC CCTCCACAGG CCAAGGCCCA TCCAAAACAC AAGACGCAAA AGCGAACGCA 
GCTGCTGCGC ATTTAGACCC CCTCGGTGAA CACGGGCTGG AGCAGCCGCT GGATGAGCAC 
GACACCGAGC ATGAGGGCGA GGACACGCCT CGAAACAGTG GCGTCATGAT CCACATGGTT 
CCGGAGACGG GGCGTGCGCG GTGGAACCAC ATCGAGGACC TTGACTCGTT CTTCTCGCGA 
ATGTATCAGT ACCAGCAGAA GCACGGATTC ACAGTGATTG TAGTGGACGA AATGCTACAG 
GTTCTGGAGT TTGGTTTCGT CGTCTGGCTA CTTGCCTTTG TGATGCACTG CGTCCGATTT 
GACGTGCTCT TTGGGGACAC CCCTCCAGGG GGACTTAACC CCAACAAGAC AACTTTATCC 
GATGTGATGT ACCCCACAGG CGAGTGCTTA GCCAACTTCA CTTGGGTCAC GTACCTGGTT 
GTGTTCATAG CCGCTATCTA TTTGGGCATA AGGCTTCTGA AAATGGTGTA CCATATTACC 
CAGTACGCCG ATATAAAGAG ATTCTACAAC TCTGCGCTTC ATATCGAGGA TTCGGATCTG 
GACAACTTTA CGTGGCACGA GGTGCAACAG CGGATTCGTC GCGTCCAGGC CGAACAGCAT 
ATGTGCATCG ACAAGGAGTC GCTTACGGAA TTGGACATCT ATCACCGAGT TCTGCGCTTC 
AAGAACTACC TAGTGGCACT GATGAACAAG CAACTGCTGC CTGTAAGGTT CCACATACCG 
CTGTATGGCG AAGTGGTCTC GCTGAGTAGA GGTATGTTGT TCAACATTGA CTTCATCTTG 
TTTCGCGGCC CTGGATCTCC CTTCCAGAAC AATTGGCAGC TGCGTGATGA GTTTGCGGTA 
AGAAGCAATC AAACGGAGCT GGCGCAGCGA CTCTCCAAAC TGATATTGGG GGTGGCGTTG 
TTAAATTTGG TCCTAGCGCC TGTGATCTTC GTATGGCAAC TTATCTACTT CAGTTTCTCC 
TATGCGAACA TTCTTCGTAA GGAGCCTGGT GCCCTTGGCC TGCGTACATG GTCCAATTAC 
GGCCGCCTCT ACCTGCGCCA CTTTAATGAA CTGGACCACG AACTAGATGC CAGGCTGAAT 
CGGGCATATG ACTACGCGGA CCGCTATCTC AACTCCTTTT CCTCGCCACT GGCCGCTGTG 
ATAGCCAAAA ACCTACTCTT TATCAGTGGA GGACTACTAC TTCTAATCCT TGCATTGGGC 
ATTTACGAGG AACACGTCTT CCAGGTGGAG CATTTGCTTG CCATCCTTGC AGGGCTCGGC 
GCCATAGGTG TGGTCTGCAG AACTCTAATA CCGGATGAGA ATCTCGTTTG GTGCCCAGAG 
CAGCTAATGA CCGCCATTCT GGCGCACGTT CACTACCTGC CCTCCGAGTG GCGGCAGCAG 
GCGCACACGA CAAAGGTTCG CCAGGAGTTT AGCAACTTCT TTCAGTTTAA GGCTGGCTAC 
CTACTCAGCG AAATCTTCAG TCCCTTCGTC ACACCCTTCG TGCTGATCTT TGTGTTCAGG 
CCAAAGGCCA TAGAGCTGGT GCGTTTCTTC AGGACGTTCA CCGTATCCGT GCGAGGAGTG 
GGCAACGTAT GCTCCTTTGC CCAGATGGAT GTTCGCAAGC ATGGCAATCC CGACTGGCAG 
TTAACCAGCG AACTGGAGGA GATGACCCGG GCTACAGCTC AACAGCCGCA GCAGGAGCCA 
CAGCAGCAAA GTTTGGCCGG CGGCAAAACG GAGATGTCGC TTCTTCGCTT CACCCTGAAC 
AATCCCGAGT GGCAGATGCC CAAGGAGGCC AAGCAGTTCC TGCGCGGCGT TAGAGAGCAC 
GCCGTTGGTG AACTAGTCCA GGCCAAGACG TCGATGGTGC AGGAGAACCC GCTGACCAAC 
AGCCTCATCT CATTTGGCAC CATGGGAGCG GATTATTGTT CCATTGCGAA CTCTGTGCTG 
ACCGCACAGG TGACGCCCCA GCAACTGGAG ATTTCGCAGT CGTTGCGTCC GGGTCTAGGA 
CCGGTATCTG GAGGATTCCC CGTCGCAGCC AGCGACTTCC GCCAGATGTT GCAGCAGAAT 
CTGTCCGCCA GTGTCGGCCC GCTGGACAGC ATGCGCCGAT TGCGCCTCAG CCGGGCTGAA 
GGCCGCTTGG AGGGTCCGAC GGATACACTG CTCTACGGAC TCTGTGGTGT GGACCCGCGC 
GTGGGCAGCA CTCCCTTGAA TGTGGGCGTG GCCGACATGT GCCTCAGTGC CCTCTATTTG 
CACGAGCTGA ACCAGCAGAA GCGCCAAGCG CGCCAGTCTC GCATCGACGA GGCCGAGGAC 
GAGCGTCCTG GTACGAGCCA TTGGCCACCG CGACCACCAG CTGCTCCGTC GGCTGATACT 
GGCTTCGGCT CCCGCCATAC CGTGATTACC TCAAAGGCTG CCGAGAGCAC GCCGCTTTTG 
GGTAGCATCC GCTCATAGCA GCGGCCAGAG TGGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261023.1
CDS	122..2680
Translation	MSSPHINYRSLAEEAASPFLEHHPSTGQGPSKTQDAKANAAAAHLDPLGEHGLEQPLDEHDTEHEGEDTPRNSGVMIHMVPETGRARWNHIEDLDSFFSRMYQYQQKHGFTVIVVDEMLQVLEFGFVVWLLAFVMHCVRFDVLFGDTPPGGLNPNKTTLSDVMYPTGECLANFTWVTYLVVFIAAIYLGIRLLKMVYHITQYADIKRFYNSALHIEDSDLDNFTWHEVQQRIRRVQAEQHMCIDKESLTELDIYHRVLRFKNYLVALMNKQLLPVRFHIPLYGEVVSLSRGMLFNIDFILFRGPGSPFQNNWQLRDEFAVRSNQTELAQRLSKLILGVALLNLVLAPVIFVWQLIYFSFSYANILRKEPGALGLRTWSNYGRLYLRHFNELDHELDARLNRAYDYADRYLNSFSSPLAAVIAKNLLFISGGLLLLILALGIYEEHVFQVEHLLAILAGLGAIGVVCRTLIPDENLVWCPEQLMTAILAHVHYLPSEWRQQAHTTKVRQEFSNFFQFKAGYLLSEIFSPFVTPFVLIFVFRPKAIELVRFFRTFTVSVRGVGNVCSFAQMDVRKHGNPDWQLTSELEEMTRATAQQPQQEPQQQSLAGGKTEMSLLRFTLNNPEWQMPKEAKQFLRGVREHAVGELVQAKTSMVQENPLTNSLISFGTMGADYCSIANSVLTAQVTPQQLEISQSLRPGLGPVSGGFPVAASDFRQMLQQNLSASVGPLDSMRRLRLSRAEGRLEGPTDTLLYGLCGVDPRVGSTPLNVGVADMCLSALYLHELNQQKRQARQSRIDEAEDERPGTSHWPPRPPAAPSADTGFGSRHTVITSKAAESTPLLGSIRS*QRPEWI

Target ORF information:

RefSeq Version	NM_001274094.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 9 (Atg9), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274094.2

ORF Insert Sequence:

ATGTCTAGCC CACATATCAA CTACCGCTCC CTGGCCGAGG AGGCGGCCAG TCCGTTCCTG 
GAGCACCATC CCTCCACAGG CCAAGGCCCA TCCAAAACAC AAGACGCAAA AGCGAACGCA 
GCTGCTGCGC ATTTAGACCC CCTCGGTGAA CACGGGCTGG AGCAGCCGCT GGATGAGCAC 
GACACCGAGC ATGAGGGCGA GGACACGCCT CGAAACAGTG GCGTCATGAT CCACATGGTT 
CCGGAGACGG GGCGTGCGCG GTGGAACCAC ATCGAGGACC TTGACTCGTT CTTCTCGCGA 
ATGTATCAGT ACCAGCAGAA GCACGGATTC ACAGTGATTG TAGTGGACGA AATGCTACAG 
GTTCTGGAGT TTGGTTTCGT CGTCTGGCTA CTTGCCTTTG TGATGCACTG CGTCCGATTT 
GACGTGCTCT TTGGGGACAC CCCTCCAGGG GGACTTAACC CCAACAAGAC AACTTTATCC 
GATGTGATGT ACCCCACAGG CGAGTGCTTA GCCAACTTCA CTTGGGTCAC GTACCTGGTT 
GTGTTCATAG CCGCTATCTA TTTGGGCATA AGGCTTCTGA AAATGGTGTA CCATATTACC 
CAGTACGCCG ATATAAAGAG ATTCTACAAC TCTGCGCTTC ATATCGAGGA TTCGGATCTG 
GACAACTTTA CGTGGCACGA GGTGCAACAG CGGATTCGTC GCGTCCAGGC CGAACAGCAT 
ATGTGCATCG ACAAGGAGTC GCTTACGGAA TTGGACATCT ATCACCGAGT TCTGCGCTTC 
AAGAACTACC TAGTGGCACT GATGAACAAG CAACTGCTGC CTGTAAGGTT CCACATACCG 
CTGTATGGCG AAGTGGTCTC GCTGAGTAGA GGTATGTTGT TCAACATTGA CTTCATCTTG 
TTTCGCGGCC CTGGATCTCC CTTCCAGAAC AATTGGCAGC TGCGTGATGA GTTTGCGGTA 
AGAAGCAATC AAACGGAGCT GGCGCAGCGA CTCTCCAAAC TGATATTGGG GGTGGCGTTG 
TTAAATTTGG TCCTAGCGCC TGTGATCTTC GTATGGCAAC TTATCTACTT CAGTTTCTCC 
TATGCGAACA TTCTTCGTAA GGAGCCTGGT GCCCTTGGCC TGCGTACATG GTCCAATTAC 
GGCCGCCTCT ACCTGCGCCA CTTTAATGAA CTGGACCACG AACTAGATGC CAGGCTGAAT 
CGGGCATATG ACTACGCGGA CCGCTATCTC AACTCCTTTT CCTCGCCACT GGCCGCTGTG 
ATAGCCAAAA ACCTACTCTT TATCAGTGGA GGACTACTAC TTCTAATCCT TGCATTGGGC 
ATTTACGAGG AACACGTCTT CCAGGTGGAG CATTTGCTTG CCATCCTTGC AGGGCTCGGC 
GCCATAGGTG TGGTCTGCAG AACTCTAATA CCGGATGAGA ATCTCGTTTG GTGCCCAGAG 
CAGCTAATGA CCGCCATTCT GGCGCACGTT CACTACCTGC CCTCCGAGTG GCGGCAGCAG 
GCGCACACGA CAAAGGTTCG CCAGGAGTTT AGCAACTTCT TTCAGTTTAA GGCTGGCTAC 
CTACTCAGCG AAATCTTCAG TCCCTTCGTC ACACCCTTCG TGCTGATCTT TGTGTTCAGG 
CCAAAGGCCA TAGAGCTGGT GCGTTTCTTC AGGACGTTCA CCGTATCCGT GCGAGGAGTG 
GGCAACGTAT GCTCCTTTGC CCAGATGGAT GTTCGCAAGC ATGGCAATCC CGACTGGCAG 
TTAACCAGCG AACTGGAGGA GATGACCCGG GCTACAGCTC AACAGCCGCA GCAGGAGCCA 
CAGCAGCAAA GTTTGGCCGG CGGCAAAACG GAGATGTCGC TTCTTCGCTT CACCCTGAAC 
AATCCCGAGT GGCAGATGCC CAAGGAGGCC AAGCAGTTCC TGCGCGGCGT TAGAGAGCAC 
GCCGTTGGTG AACTAGTCCA GGCCAAGACG TCGATGGTGC AGGAGAACCC GCTGACCAAC 
AGCCTCATCT CATTTGGCAC CATGGGAGCG GATTATTGTT CCATTGCGAA CTCTGTGCTG 
ACCGCACAGG TGACGCCCCA GCAACTGGAG ATTTCGCAGT CGTTGCGTCC GGGTCTAGGA 
CCGGTATCTG GAGGATTCCC CGTCGCAGCC AGCGACTTCC GCCAGATGTT GCAGCAGAAT 
CTGTCCGCCA GTGTCGGCCC GCTGGACAGC ATGCGCCGAT TGCGCCTCAG CCGGGCTGAA 
GGCCGCTTGG AGGGTCCGAC GGATACACTG CTCTACGGAC TCTGTGGTGT GGACCCGCGC 
GTGGGCAGCA CTCCCTTGAA TGTGGGCGTG GCCGACATGT GCCTCAGTGC CCTCTATTTG 
CACGAGCTGA ACCAGCAGAA GCGCCAAGCG CGCCAGTCTC GCATCGACGA GGCCGAGGAC 
GAGCGTCCTG GTACGAGCCA TTGGCCACCG CGACCACCAG CTGCTCCGTC GGCTGATACT 
GGCTTCGGCT CCCGCCATAC CGTGATTACC TCAAAGGCTG CCGAGAGCAC GCCGCTTTTG 
GGTAGCATCC GCTCATAGCA GCGGCCAGAG TGGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Atg9 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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