cry cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	cry
Entrez Gene ID	42305
Full Name	cryptochrome
Synonyms	CG3772,CRY,Cry,DCry,Dm-CRY1,DmCRY,DmCRY1,DmCry,DmCry1,Dmcry,Dmel\CG3772,anon-WO0140519.17,anon-WO0140519.19,anon-WO0140519.20,anon-WO0172774.15,cryb,dCRY,dCry,dcry
General protein information	Preferred Names cryptochrome Names CG3772 gene product from transcript CG3772-RA Blue-light receptor CG3772-PA cry-PA crybaby
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91F11-91F11

mRNA and Protein(s)

mRNA	Protein	Name
NM_169852.2	NP_732407.1	cryptochrome

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	cry	NP_732407.1
Anopheles gambiae (African malaria mosquito)	CRY1_ANOGA	XP_321104.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CRY is not stabilized by interaction with the kinase Shaggy (SGG), the GSK-3 beta fly orthologue.
Title: GSK-3 Beta Does Not Stabilize Cryptochrome in the Circadian Clock of Drosophila.

Using time-resolved and steady-state optical spectroscopy, we studied the mechanism of light-induced radical-pair formation and decay, and the photoreduction of the FAD cofactor.
Title: Spectroscopic characterization of radicals and radical pairs in fruit fly cryptochrome - protonated and nonprotonated flavin radical-states.

The results of this study concluded that the E oscillators are the targets of light input via CRY and the visual system and are required for normal light entrainment.
Title: Cryptochrome-dependent and -independent circadian entrainment circuits in Drosophila.

our studies provided novel evidence that the circadian clock gene, dCry, plays an essential role in heart morphogenesis and function.
Title: A Circadian Clock Gene, Cry, Affects Heart Morphogenesis and Function in Drosophila as Revealed by Optical Coherence Microscopy.

The Cry leucine-histidine substitution is common in Drosophila, with both alleles at intermediate frequencies across 27 populations surveyed in Europe, irrespective of latitude.
Title: Molecular evolution of a pervasive natural amino-acid substitution in Drosophila cryptochrome.

The following cry gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cry cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05388	NM_169852.2 Latest version!	Drosophila melanogaster cryptochrome (cry), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cry ( NM_169852.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732407.1 Drosophila melanogaster cryptochrome (cry), mRNA.

Price & Availability↑

CloneID	OFa05388
Clone ID Related Accession (Same CDS sequence)	NM_169852.2
Accession Version	NM_169852.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cryptochrome
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169852.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACGC GAGGGGCGAA TGTGATTTGG TTTCGCCATG GATTGCGCCT CCATGATAAT 
CCCGCTCTAT TGGCCGCCCT CGCCGATAAG GATCAGGGTA TAGCCCTAAT TCCCGTTTTC 
ATATTCGATG GAGAGAGTGC AGGTACCAAG AATGTGGGTT ACAATCGGAT GCGTTTCCTC 
CTGGACTCGT TGCAGGACAT CGATGATCAG CTACAGGCGG CAACTGATGG ACGTGGACGC 
CTCCTGGTCT TCGAGGGCGA ACCGGCTTAT ATCTTCCGCC GGCTACATGA GCAAGTGCGT 
CTGCACAGGA TTTGCATAGA GCAGGACTGC GAGCCAATTT GGAATGAGCG CGATGAAAGC 
ATCCGTTCTC TATGTCGGGA GCTGAATATC GACTTTGTCG AGAAGGTATC ACACACGCTT 
TGGGATCCGC AATTGGTGAT TGAGACCAAT GGTGGCATTC CACCGCTGAC CTACCAAATG 
TTCCTGCACA CGGTGCAAAT TATTGGGCTT CCACCGCGTC CCACCGCCGA TGCTCGACTA 
GAAGACGCCA CCTTTGTCGA GCTGGACCCC GAGTTCTGCC GAAGTCTTAA GTTGTTCGAG 
CAGCTGCCCA CGCCGGAGCA CTTCAATGTG TATGGAGACA ACATGGGCTT CCTGGCCAAG 
ATTAACTGGC GCGGCGGAGA AACACAGGCC TTACTTCTGT TGGATGAGCG TCTTAAAGTG 
GAGCAGCATG CGTTTGAGCG TGGATTTTAT CTGCCCAACC AGGCACTGCC CAATATCCAC 
GACTCGCCAA AATCGATGAG CGCCCATCTG CGCTTTGGTT GCCTTTCGGT ACGTCGCTTC 
TACTGGAGCG TCCACGATCT CTTCAAGAAT GTCCAGTTGC GCGCCTGTGT GCGGGGCGTT 
CAGATGACTG GCGGCGCGCA CATCACGGGA CAGTTGATCT GGCGAGAGTA CTTCTACACC 
ATGTCGGTGA ACAATCCAAA CTACGATCGC ATGGAGGGCA ATGACATCTG CCTGAGCATC 
CCGTGGGCTA AGCCGAACGA AAATCTCCTG CAGAGCTGGC GTTTAGGCCA AACGGGATTC 
CCGCTCATCG ACGGCGCCAT GCGACAACTC CTGGCCGAGG GATGGCTCCA CCATACGCTG 
CGCAACACCG TGGCCACCTT TCTCACGCGC GGCGGTTTGT GGCAGAGCTG GGAGCATGGA 
CTGCAGCACT TTCTGAAGTA TCTGCTGGAT GCGGATTGGT CGGTCTGCGC TGGCAACTGG 
ATGTGGGTAT CCAGCTCGGC GTTTGAAAGG CTGCTGGACT CCTCCCTGGT CACCTGCCCG 
GTGGCATTGG CCAAGCGACT TGATCCGGAT GGCACCTACA TCAAGCAGTA CGTCCCGGAG 
TTGATGAATG TGCCCAAGGA ATTTGTTCAC GAGCCCTGGC GAATGTCTGC CGAGCAGCAG 
GAGCAGTACG AGTGCCTGAT CGGAGTCCAT TATCCGGAGC GGATCATTGA TTTGTCCATG 
GCCGTAAAGC GAAACATGCT GGCCATGAAG TCTCTCCGAA ATTCGCTGAT CACCCCGCCG 
CCGCATTGCC GACCATCCAA CGAGGAGGAA GTGCGTCAGT TCTTCTGGCT GGCCGACGTG 
GTGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732407.1
CDS	188..1816
Translation	MATRGANVIWFRHGLRLHDNPALLAALADKDQGIALIPVFIFDGESAGTKNVGYNRMRFLLDSLQDIDDQLQAATDGRGRLLVFEGEPAYIFRRLHEQVRLHRICIEQDCEPIWNERDESIRSLCRELNIDFVEKVSHTLWDPQLVIETNGGIPPLTYQMFLHTVQIIGLPPRPTADARLEDATFVELDPEFCRSLKLFEQLPTPEHFNVYGDNMGFLAKINWRGGETQALLLLDERLKVEQHAFERGFYLPNQALPNIHDSPKSMSAHLRFGCLSVRRFYWSVHDLFKNVQLRACVRGVQMTGGAHITGQLIWREYFYTMSVNNPNYDRMEGNDICLSIPWAKPNENLLQSWRLGQTGFPLIDGAMRQLLAEGWLHHTLRNTVATFLTRGGLWQSWEHGLQHFLKYLLDADWSVCAGNWMWVSSSAFERLLDSSLVTCPVALAKRLDPDGTYIKQYVPELMNVPKEFVHEPWRMSAEQQEQYECLIGVHYPERIIDLSMAVKRNMLAMKSLRNSLITPPPHCRPSNEEEVRQFFWLADVVV

Target ORF information:

RefSeq Version	NM_169852.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cryptochrome (cry), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169852.2

ORF Insert Sequence:

ATGGCCACGC GAGGGGCGAA TGTGATTTGG TTTCGCCATG GATTGCGCCT CCATGATAAT 
CCCGCTCTAT TGGCCGCCCT CGCCGATAAG GATCAGGGTA TAGCCCTAAT TCCCGTTTTC 
ATATTCGATG GAGAGAGTGC AGGTACCAAG AATGTGGGTT ACAATCGGAT GCGTTTCCTC 
CTGGACTCGT TGCAGGACAT CGATGATCAG CTACAGGCGG CAACTGATGG ACGTGGACGC 
CTCCTGGTCT TCGAGGGCGA ACCGGCTTAT ATCTTCCGCC GGCTACATGA GCAAGTGCGT 
CTGCACAGGA TTTGCATAGA GCAGGACTGC GAGCCAATTT GGAATGAGCG CGATGAAAGC 
ATCCGTTCTC TATGTCGGGA GCTGAATATC GACTTTGTCG AGAAGGTATC ACACACGCTT 
TGGGATCCGC AATTGGTGAT TGAGACCAAT GGTGGCATTC CACCGCTGAC CTACCAAATG 
TTCCTGCACA CGGTGCAAAT TATTGGGCTT CCACCGCGTC CCACCGCCGA TGCTCGACTA 
GAAGACGCCA CCTTTGTCGA GCTGGACCCC GAGTTCTGCC GAAGTCTTAA GTTGTTCGAG 
CAGCTGCCCA CGCCGGAGCA CTTCAATGTG TATGGAGACA ACATGGGCTT CCTGGCCAAG 
ATTAACTGGC GCGGCGGAGA AACACAGGCC TTACTTCTGT TGGATGAGCG TCTTAAAGTG 
GAGCAGCATG CGTTTGAGCG TGGATTTTAT CTGCCCAACC AGGCACTGCC CAATATCCAC 
GACTCGCCAA AATCGATGAG CGCCCATCTG CGCTTTGGTT GCCTTTCGGT ACGTCGCTTC 
TACTGGAGCG TCCACGATCT CTTCAAGAAT GTCCAGTTGC GCGCCTGTGT GCGGGGCGTT 
CAGATGACTG GCGGCGCGCA CATCACGGGA CAGTTGATCT GGCGAGAGTA CTTCTACACC 
ATGTCGGTGA ACAATCCAAA CTACGATCGC ATGGAGGGCA ATGACATCTG CCTGAGCATC 
CCGTGGGCTA AGCCGAACGA AAATCTCCTG CAGAGCTGGC GTTTAGGCCA AACGGGATTC 
CCGCTCATCG ACGGCGCCAT GCGACAACTC CTGGCCGAGG GATGGCTCCA CCATACGCTG 
CGCAACACCG TGGCCACCTT TCTCACGCGC GGCGGTTTGT GGCAGAGCTG GGAGCATGGA 
CTGCAGCACT TTCTGAAGTA TCTGCTGGAT GCGGATTGGT CGGTCTGCGC TGGCAACTGG 
ATGTGGGTAT CCAGCTCGGC GTTTGAAAGG CTGCTGGACT CCTCCCTGGT CACCTGCCCG 
GTGGCATTGG CCAAGCGACT TGATCCGGAT GGCACCTACA TCAAGCAGTA CGTCCCGGAG 
TTGATGAATG TGCCCAAGGA ATTTGTTCAC GAGCCCTGGC GAATGTCTGC CGAGCAGCAG 
GAGCAGTACG AGTGCCTGAT CGGAGTCCAT TATCCGGAGC GGATCATTGA TTTGTCCATG 
GCCGTAAAGC GAAACATGCT GGCCATGAAG TCTCTCCGAA ATTCGCTGAT CACCCCGCCG 
CCGCATTGCC GACCATCCAA CGAGGAGGAA GTGCGTCAGT TCTTCTGGCT GGCCGACGTG 
GTGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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cry cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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