Ask1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ask1
Entrez Gene ID	42366
Full Name	Apoptotic signal-regulating kinase 1
Synonyms	ASK1,CG4720,D-ASK,DASK,DASK1,DPk92B/ASK1,Dmel\CG4720,MEKK5/ASK1,Pk92B,dASK,dASK1,dAsk1,pk92B
General protein information	Preferred Names Apoptotic signal-regulating kinase 1 Names CG4720 gene product from transcript CG4720-RB Ask1-PB Ask1-PC CG4720-PB CG4720-PC apoptotic signal-regulating kinase 1 protein kinase at 92B
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 92B4-92B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_057741.4	NP_477089.3	apoptotic signal-regulating kinase 1, isoform C
NM_206518.2	NP_996240.1	apoptotic signal-regulating kinase 1, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	MAP3K15	NP_001001671.3
Bos taurus (cattle)	MAP3K15	XP_002700399.1
Rattus norvegicus (Norway rat)	Map3k15	XP_006256994.1
Gallus gallus (chicken)	MAP3K15	XP_003640537.1
Drosophila melanogaster (fruit fly)	Pk92B	NP_477089.3
	LOC695958	XP_002808565.1
Canis lupus familiaris (dog)	MAP3K15	XP_548885.3
Mus musculus (house mouse)	Map3k15	NP_001156557.2
Danio rerio (zebrafish)	map3k15	XP_003201359.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000747	XP_311281.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Suggest that Slim/KLHDC10 is an activator of ASK1, contributing to oxidative stress-induced cell death through the suppression of PP5.
Title: The Kelch repeat protein KLHDC10 regulates oxidative stress-induced ASK1 activation by suppressing PP5.

The following Ask1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ask1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08538	NM_206518.2 Latest version!	Drosophila melanogaster apoptotic signal-regulating kinase 1, transcript variant B (Ask1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa08539	NM_057741.4 Latest version!	Drosophila melanogaster apoptotic signal-regulating kinase 1, transcript variant C (Ask1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ask1 ( NM_206518.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996240.1 Drosophila melanogaster apoptotic signal-regulating kinase 1, transcript variant B (Ask1), mRNA.

Price & Availability↑

CloneID	OFa08538
Clone ID Related Accession (Same CDS sequence)	NM_206518.2
Accession Version	NM_206518.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	apoptotic signal-regulating kinase 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_206518.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTGAGA AATTTCTGAG TGACATGCGA ACGGCGCGGG ACACGTACGC CACCAACGGC 
GCCAAGCTGC AGAGTATATT GCACGAGATG CGAAAGCGGC TGGACGATCC GCATGTCCTG 
TCTGGCGAAG TGGTACACAG TTTCATGTGC TCGCTGAGGG ATGTGCAGGA CTACGATGCC 
ATGGTGCGAC TGGTCAATGA CCTGAAGAAC ATACCCAACA CCCGGAAGTA CGTGGAGACG 
GGCAACATGA GTTTCCTGTA CGCCTTCGCC CTGAACCGAA GAAATCGAAA GGGAGACCGT 
GAAAAGGCTC TGGAATCTTC GCTGAAGGCG CTGGAAAAGA AGGAGAACCA CTTTCCGGAC 
ATGCTCTGCC TGTGCGGCCG CATCTACAAG GACATCTTTG TGGAATCCGA CTACACGGAC 
GCCACCAGTT TGGCGCACGC CATCAAGTGG TACCGCCAAA GCTTTGAGGT GCAGCCGAAT 
GAGTATGCAG GCATCAATCT GGCTACTCTG CTGGTTATCG AGGGCAAGGA GTTTACCAAT 
ACGGAGGAGT TGCAGCACAT CGGCATGACC TTAAACAATT TAATCGGTAA AAAGGGCAGC 
CTATCCTCGC TGAGTGAGTA TTGGGATGTG GCCACCTTCT TTGAGATCTC CGTTTTGGCC 
AACGACTACG CCAAGGCGAT TCAAGCTGCC GAGTGCATGT TCAAGCTAAA GCCACCCAAC 
TGGTATCTGA AGTCGACCAT CGGAAATATC TCTCTTATAC ATCGATTTCG AAAGAAGCCC 
GAGGAACGCC AGCCGCCCAT CGAGGAGCAG GTGTTCCAGT TCTGGATGGA CTTCTTTCTG 
GAGGCCACCA ACACCGAGGA GGTTAAGAAC AGCATTCGCT TTCCGATTCT GATCCTCGAG 
CCTCAGAAGA TTTATATGCC CAGTTATGTG ACTATAAACA TGGATGCGGA TGAGAAGTCC 
ATTCAGATTG TGAACATCTG TTTGGCTCAC GCAAAGAATG CGTGTAAGAA AATCCACGAC 
TTCCTTTTCG TGGCATCGAA GATCAAGTCC GTCAGCCTTT ACAAGAGGGA TGATCGTTGT 
GCCTACTTGT ACGTCCATCA CAACTCAGAT GATTTTCAAA TATATTTTCC CTCCACTGAA 
CGACGACAAA AGTTCTACGA TATGATCCTG GAGATGACTG CAGATCAGGT GGTGTTTGTC 
AATCTCAGCA ATGATGACGC TAATATCGAG TACGAGTACG ACTACGATGA GCAGAATCGC 
AAAATGGTGC TGGGCAAAGG TACTTATGGC ACCGTGTACG CCGCCAGGGA CAAGCAGACG 
CAGGTGCGAA TCGCCATTAA GGAGGTGCCG GAAAAGAACT CGCAGGATGT GCAGCCACTG 
CACGAGGAGA TTAAGTTACA CTCTCAGCTG CGACATCGCA ACATAGTTCG CTATTTGGGA 
TCGTGCTCGG AGAACGGCTT CTTCAAGATC TTCATGGAGC AGGTGCCGGG AGGATCGCTT 
TCCGACCTCT TAGAGACCAA ATGGGGTCCG CTCAAGGACA ACGAGTCCAC CATGGCCTTC 
TACTCCAAGC AGATCCTCGA GGGGCTGAAG TACCTTCACG AGCAGGACAT TGTTCATCGC 
GATATAAAGG GTGACAACGT GCTGGTTAAC ACGTATAGCG GCGTGGTTAA GATCTCCGAT 
TTCGGCACCT CCAAGCGGCT GGCCCGCATC AACCCGATGA CGGAGACCTT TACGGGCACC 
TTGCAGTACA TGGCGCCGGA GGTGATTGAC CAGGGAGTAC GCGGCTATGG CCCGGCGGCT 
GACATCTGGT CCTTTGGATG CACAAACGTT GAGATGGCTA CGGGCAAACC ACCGTTTATC 
GAGCTGGGTA GCGCCCATGC GGCCATGTTC AAGGTGGGCT TTTACAAGAA GCATCCGAAC 
ATACCCGAGG AGCTGTCCGC CAACGCAAAG AACTTTATTT TGCGTTGCTT TGCCATCAGC 
GTGATGGACC GACCCTCCGC CTCGCAACTT CTGGAAGATC CCTTCCTACA AGATAAACCC 
CGCAAGGTGC GCCCAGCCCT GCCCATCAAT ACTGAGTTCG GACGCAGCAT TTCGGTGCCG 
GCTGATCGGT TTGTGCACAA AACCACACCG CCGCTGAGCT ACAACACAAC CTGCAATACG 
CCCACAACGC CGGAATTGGA CATAACGCAT TCATCCTCGG TGGACATTGA CGAGCTGCCC 
AGCAACCAGT TTATGCTTGA ACGGCGAAAT TCCCATGGAT TTTTGCTCTC GCCAGAGATT 
GAACCTTCCA CGCCGTCCTT GCGTACGAGC ATTAGTGAGA CGAGCGAGAC TGACGGTTTT 
TACAGGCTTA AAAAGGATTC GCAGAGGCGG ACAACGCTTT CCAAGGTGCT GGCACTAGAC 
GAATCCAAGA TCTGCGATAT TTGGCTGAAG AAGGTCGACG CTGATCAGAA CTCGATTGCG 
ATTAGGAAAA GCGATTTGGA GGTTCTAATC CGAGGACTGC GGGACTACAT TATGAACGAA 
AACGAAAAGC ATTTGGAAGC GACTATCAAC GAACTGAAAC AAAAGCTGAA CAACGACGCA 
GTTGCTCTGG ACCATCTTCA TTTGGCGCTG TACTCCTTTC AGGACGCCGT GGTCTGCGTA 
CTGCGGTACC ATTGCATCAA ACCCCATTGG ATGTTCGCAT TGGATAATCT GGTTAAGCGG 
GCCGTCCAGG CGGCGGTCAC TATTTTCTCT CCTGAACTGG GCGCCAATCT GGCGGACAAA 
GACCTGTCTG GAAATGATGA TGAGAGCTTG CGTACAAGTC TAATGCAGAA GGGTTCCACC 
GAGAGCCAGG AAAAGCAGAG CCTGGAAAAA GTTCTTCCCA CGAGCAACAC TCGCGAAGGT 
GTGGGATTAA CGCCTGCGGA ATCGGGCGGG GATGCTCTGT CCAGTTCGAT GGAGTGCGCG 
CGCATCCTGA GAGACATCCG TGACAATCAG CAGAAGCTGC AACGCCAGCT CCTTGAGCAG 
CAGCGGCAGA GCATGAGGGC CCTGCATAAT ATCAGTCAGG AGCTGGCTCA CATTTACATG 
GGCGGCAGAA AGAGATTAAG ACGCACAATA AACGGCTTTA ACAAAAAGTG CCATAGAATG 
AGTACCAGTG TGGGCGGAAC GTCCGGAGTC CGTCCGAATA TAGCGGGCAA TCCCCGGAGG 
CAGCTAAACA AGCATCACTT TGGTCTTCAC GAAGTAGACG AGCAGTTGGA GCAGTGGTTG 
ACCCAGCAGG AAATAGACGA GTTCTCAAAG ACGCTCATCC TGAATGAAGG TTTTACATTT 
GACGACTTTA TCTATAACAT GGAAAAGCTG GACCTCATGA GGCTGGGTCT GCGCGTTGGC 
ATCGAAGTGC GTTTGTGGAA ACTCATTATA CAAGAACGTG TGTGCACCTC GTCCGATTGT 
GTGGACAGTC CACCAAGCAC TTACCACAAA CCCATTGGAG GCAATAGCTC TTTTGCGCTT 
ATTGACAACA ACAATACGCT ACCATCACCC ACAACCACAA CTCCCGGAAC GCCGGGCATC 
AAAACGAAAA TCAAGAAAAG CGAAAGCGAG TACGATTCGT GCAGCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996240.1
CDS	1279..4869
Translation	MREKFLSDMRTARDTYATNGAKLQSILHEMRKRLDDPHVLSGEVVHSFMCSLRDVQDYDAMVRLVNDLKNIPNTRKYVETGNMSFLYAFALNRRNRKGDREKALESSLKALEKKENHFPDMLCLCGRIYKDIFVESDYTDATSLAHAIKWYRQSFEVQPNEYAGINLATLLVIEGKEFTNTEELQHIGMTLNNLIGKKGSLSSLSEYWDVATFFEISVLANDYAKAIQAAECMFKLKPPNWYLKSTIGNISLIHRFRKKPEERQPPIEEQVFQFWMDFFLEATNTEEVKNSIRFPILILEPQKIYMPSYVTINMDADEKSIQIVNICLAHAKNACKKIHDFLFVASKIKSVSLYKRDDRCAYLYVHHNSDDFQIYFPSTERRQKFYDMILEMTADQVVFVNLSNDDANIEYEYDYDEQNRKMVLGKGTYGTVYAARDKQTQVRIAIKEVPEKNSQDVQPLHEEIKLHSQLRHRNIVRYLGSCSENGFFKIFMEQVPGGSLSDLLETKWGPLKDNESTMAFYSKQILEGLKYLHEQDIVHRDIKGDNVLVNTYSGVVKISDFGTSKRLARINPMTETFTGTLQYMAPEVIDQGVRGYGPAADIWSFGCTNVEMATGKPPFIELGSAHAAMFKVGFYKKHPNIPEELSANAKNFILRCFAISVMDRPSASQLLEDPFLQDKPRKVRPALPINTEFGRSISVPADRFVHKTTPPLSYNTTCNTPTTPELDITHSSSVDIDELPSNQFMLERRNSHGFLLSPEIEPSTPSLRTSISETSETDGFYRLKKDSQRRTTLSKVLALDESKICDIWLKKVDADQNSIAIRKSDLEVLIRGLRDYIMNENEKHLEATINELKQKLNNDAVALDHLHLALYSFQDAVVCVLRYHCIKPHWMFALDNLVKRAVQAAVTIFSPELGANLADKDLSGNDDESLRTSLMQKGSTESQEKQSLEKVLPTSNTREGVGLTPAESGGDALSSSMECARILRDIRDNQQKLQRQLLEQQRQSMRALHNISQELAHIYMGGRKRLRRTINGFNKKCHRMSTSVGGTSGVRPNIAGNPRRQLNKHHFGLHEVDEQLEQWLTQQEIDEFSKTLILNEGFTFDDFIYNMEKLDLMRLGLRVGIEVRLWKLIIQERVCTSSDCVDSPPSTYHKPIGGNSSFALIDNNNTLPSPTTTTPGTPGIKTKIKKSESEYDSCSE

Target ORF information:

RefSeq Version	NM_206518.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster apoptotic signal-regulating kinase 1, transcript variant B (Ask1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206518.2

ORF Insert Sequence:

ATGCGTGAGA AATTTCTGAG TGACATGCGA ACGGCGCGGG ACACGTACGC CACCAACGGC 
GCCAAGCTGC AGAGTATATT GCACGAGATG CGAAAGCGGC TGGACGATCC GCATGTCCTG 
TCTGGCGAAG TGGTACACAG TTTCATGTGC TCGCTGAGGG ATGTGCAGGA CTACGATGCC 
ATGGTGCGAC TGGTCAATGA CCTGAAGAAC ATACCCAACA CCCGGAAGTA CGTGGAGACG 
GGCAACATGA GTTTCCTGTA CGCCTTCGCC CTGAACCGAA GAAATCGAAA GGGAGACCGT 
GAAAAGGCTC TGGAATCTTC GCTGAAGGCG CTGGAAAAGA AGGAGAACCA CTTTCCGGAC 
ATGCTCTGCC TGTGCGGCCG CATCTACAAG GACATCTTTG TGGAATCCGA CTACACGGAC 
GCCACCAGTT TGGCGCACGC CATCAAGTGG TACCGCCAAA GCTTTGAGGT GCAGCCGAAT 
GAGTATGCAG GCATCAATCT GGCTACTCTG CTGGTTATCG AGGGCAAGGA GTTTACCAAT 
ACGGAGGAGT TGCAGCACAT CGGCATGACC TTAAACAATT TAATCGGTAA AAAGGGCAGC 
CTATCCTCGC TGAGTGAGTA TTGGGATGTG GCCACCTTCT TTGAGATCTC CGTTTTGGCC 
AACGACTACG CCAAGGCGAT TCAAGCTGCC GAGTGCATGT TCAAGCTAAA GCCACCCAAC 
TGGTATCTGA AGTCGACCAT CGGAAATATC TCTCTTATAC ATCGATTTCG AAAGAAGCCC 
GAGGAACGCC AGCCGCCCAT CGAGGAGCAG GTGTTCCAGT TCTGGATGGA CTTCTTTCTG 
GAGGCCACCA ACACCGAGGA GGTTAAGAAC AGCATTCGCT TTCCGATTCT GATCCTCGAG 
CCTCAGAAGA TTTATATGCC CAGTTATGTG ACTATAAACA TGGATGCGGA TGAGAAGTCC 
ATTCAGATTG TGAACATCTG TTTGGCTCAC GCAAAGAATG CGTGTAAGAA AATCCACGAC 
TTCCTTTTCG TGGCATCGAA GATCAAGTCC GTCAGCCTTT ACAAGAGGGA TGATCGTTGT 
GCCTACTTGT ACGTCCATCA CAACTCAGAT GATTTTCAAA TATATTTTCC CTCCACTGAA 
CGACGACAAA AGTTCTACGA TATGATCCTG GAGATGACTG CAGATCAGGT GGTGTTTGTC 
AATCTCAGCA ATGATGACGC TAATATCGAG TACGAGTACG ACTACGATGA GCAGAATCGC 
AAAATGGTGC TGGGCAAAGG TACTTATGGC ACCGTGTACG CCGCCAGGGA CAAGCAGACG 
CAGGTGCGAA TCGCCATTAA GGAGGTGCCG GAAAAGAACT CGCAGGATGT GCAGCCACTG 
CACGAGGAGA TTAAGTTACA CTCTCAGCTG CGACATCGCA ACATAGTTCG CTATTTGGGA 
TCGTGCTCGG AGAACGGCTT CTTCAAGATC TTCATGGAGC AGGTGCCGGG AGGATCGCTT 
TCCGACCTCT TAGAGACCAA ATGGGGTCCG CTCAAGGACA ACGAGTCCAC CATGGCCTTC 
TACTCCAAGC AGATCCTCGA GGGGCTGAAG TACCTTCACG AGCAGGACAT TGTTCATCGC 
GATATAAAGG GTGACAACGT GCTGGTTAAC ACGTATAGCG GCGTGGTTAA GATCTCCGAT 
TTCGGCACCT CCAAGCGGCT GGCCCGCATC AACCCGATGA CGGAGACCTT TACGGGCACC 
TTGCAGTACA TGGCGCCGGA GGTGATTGAC CAGGGAGTAC GCGGCTATGG CCCGGCGGCT 
GACATCTGGT CCTTTGGATG CACAAACGTT GAGATGGCTA CGGGCAAACC ACCGTTTATC 
GAGCTGGGTA GCGCCCATGC GGCCATGTTC AAGGTGGGCT TTTACAAGAA GCATCCGAAC 
ATACCCGAGG AGCTGTCCGC CAACGCAAAG AACTTTATTT TGCGTTGCTT TGCCATCAGC 
GTGATGGACC GACCCTCCGC CTCGCAACTT CTGGAAGATC CCTTCCTACA AGATAAACCC 
CGCAAGGTGC GCCCAGCCCT GCCCATCAAT ACTGAGTTCG GACGCAGCAT TTCGGTGCCG 
GCTGATCGGT TTGTGCACAA AACCACACCG CCGCTGAGCT ACAACACAAC CTGCAATACG 
CCCACAACGC CGGAATTGGA CATAACGCAT TCATCCTCGG TGGACATTGA CGAGCTGCCC 
AGCAACCAGT TTATGCTTGA ACGGCGAAAT TCCCATGGAT TTTTGCTCTC GCCAGAGATT 
GAACCTTCCA CGCCGTCCTT GCGTACGAGC ATTAGTGAGA CGAGCGAGAC TGACGGTTTT 
TACAGGCTTA AAAAGGATTC GCAGAGGCGG ACAACGCTTT CCAAGGTGCT GGCACTAGAC 
GAATCCAAGA TCTGCGATAT TTGGCTGAAG AAGGTCGACG CTGATCAGAA CTCGATTGCG 
ATTAGGAAAA GCGATTTGGA GGTTCTAATC CGAGGACTGC GGGACTACAT TATGAACGAA 
AACGAAAAGC ATTTGGAAGC GACTATCAAC GAACTGAAAC AAAAGCTGAA CAACGACGCA 
GTTGCTCTGG ACCATCTTCA TTTGGCGCTG TACTCCTTTC AGGACGCCGT GGTCTGCGTA 
CTGCGGTACC ATTGCATCAA ACCCCATTGG ATGTTCGCAT TGGATAATCT GGTTAAGCGG 
GCCGTCCAGG CGGCGGTCAC TATTTTCTCT CCTGAACTGG GCGCCAATCT GGCGGACAAA 
GACCTGTCTG GAAATGATGA TGAGAGCTTG CGTACAAGTC TAATGCAGAA GGGTTCCACC 
GAGAGCCAGG AAAAGCAGAG CCTGGAAAAA GTTCTTCCCA CGAGCAACAC TCGCGAAGGT 
GTGGGATTAA CGCCTGCGGA ATCGGGCGGG GATGCTCTGT CCAGTTCGAT GGAGTGCGCG 
CGCATCCTGA GAGACATCCG TGACAATCAG CAGAAGCTGC AACGCCAGCT CCTTGAGCAG 
CAGCGGCAGA GCATGAGGGC CCTGCATAAT ATCAGTCAGG AGCTGGCTCA CATTTACATG 
GGCGGCAGAA AGAGATTAAG ACGCACAATA AACGGCTTTA ACAAAAAGTG CCATAGAATG 
AGTACCAGTG TGGGCGGAAC GTCCGGAGTC CGTCCGAATA TAGCGGGCAA TCCCCGGAGG 
CAGCTAAACA AGCATCACTT TGGTCTTCAC GAAGTAGACG AGCAGTTGGA GCAGTGGTTG 
ACCCAGCAGG AAATAGACGA GTTCTCAAAG ACGCTCATCC TGAATGAAGG TTTTACATTT 
GACGACTTTA TCTATAACAT GGAAAAGCTG GACCTCATGA GGCTGGGTCT GCGCGTTGGC 
ATCGAAGTGC GTTTGTGGAA ACTCATTATA CAAGAACGTG TGTGCACCTC GTCCGATTGT 
GTGGACAGTC CACCAAGCAC TTACCACAAA CCCATTGGAG GCAATAGCTC TTTTGCGCTT 
ATTGACAACA ACAATACGCT ACCATCACCC ACAACCACAA CTCCCGGAAC GCCGGGCATC 
AAAACGAAAA TCAAGAAAAG CGAAAGCGAG TACGATTCGT GCAGCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ask1 ( NM_057741.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477089.3 Drosophila melanogaster apoptotic signal-regulating kinase 1, transcript variant C (Ask1), mRNA.

Price & Availability↑

CloneID	OFa08539
Clone ID Related Accession (Same CDS sequence)	NM_057741.4
Accession Version	NM_057741.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	apoptotic signal-regulating kinase 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_057741.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGTGG TGTGTGTAAT GGATACGGCG GTGGGCGACC ATCTGGAGGA CCGCCAGTGT 
GCGCTGGAGG AGATCAAGCA GGCAGTGCAG TCGGCGGGCG CCAACTTTCA GCGGGTTCAG 
TTCGAGCGCC TGGACTTCGG CGAGACGAAT GTGCTGGAGA CGTTTTACAA CGCCGATGTG 
GCCATTATCG ACCTGAGCAT TCTCACCCAG CAGCGGCCGC TTTCCTATCA CTACGGCGTT 
AGGGAGAGCT TCGGGATGAA GGAGAACATC CTCACCTACA ACGACATCGA TTCCAAGCAG 
ACGCTCAGTC TCAAGCTCTC GTGTGCCAAC TACCTGTTTC TCAGCTACAA GCGGAATGCG 
GAGACCAATT CCTGCCACCT GACCAGCCAG CCCAACAGCG GCAACAACAG CAAGGAACCC 
AATGCCGAAG GACGAGTGCC CACTTTGCAG TGGCGCCTCA AGCGAAAGCT GCAAGATGTT 
GAGATCCAGT CGAAAGCCCA CATGCGTGAG AAATTTCTGA GTGACATGCG AACGGCGCGG 
GACACGTACG CCACCAACGG CGCCAAGCTG CAGAGTATAT TGCACGAGAT GCGAAAGCGG 
CTGGACGATC CGCATGTCCT GTCTGGCGAA GTGGTACACA GTTTCATGTG CTCGCTGAGG 
GATGTGCAGG ACTACGATGC CATGGTGCGA CTGGTCAATG ACCTGAAGAA CATACCCAAC 
ACCCGGAAGT ACGTGGAGAC GGGCAACATG AGTTTCCTGT ACGCCTTCGC CCTGAACCGA 
AGAAATCGAA AGGGAGACCG TGAAAAGGCT CTGGAATCTT CGCTGAAGGC GCTGGAAAAG 
AAGGAGAACC ACTTTCCGGA CATGCTCTGC CTGTGCGGCC GCATCTACAA GGACATCTTT 
GTGGAATCCG ACTACACGGA CGCCACCAGT TTGGCGCACG CCATCAAGTG GTACCGCCAA 
AGCTTTGAGG TGCAGCCGAA TGAGTATGCA GGCATCAATC TGGCTACTCT GCTGGTTATC 
GAGGGCAAGG AGTTTACCAA TACGGAGGAG TTGCAGCACA TCGGCATGAC CTTAAACAAT 
TTAATCGGTA AAAAGGGCAG CCTATCCTCG CTGAGTGAGT ATTGGGATGT GGCCACCTTC 
TTTGAGATCT CCGTTTTGGC CAACGACTAC GCCAAGGCGA TTCAAGCTGC CGAGTGCATG 
TTCAAGCTAA AGCCACCCAA CTGGTATCTG AAGTCGACCA TCGGAAATAT CTCTCTTATA 
CATCGATTTC GAAAGAAGCC CGAGGAACGC CAGCCGCCCA TCGAGGAGCA GGTGTTCCAG 
TTCTGGATGG ACTTCTTTCT GGAGGCCACC AACACCGAGG AGGTTAAGAA CAGCATTCGC 
TTTCCGATTC TGATCCTCGA GCCTCAGAAG ATTTATATGC CCAGTTATGT GACTATAAAC 
ATGGATGCGG ATGAGAAGTC CATTCAGATT GTGAACATCT GTTTGGCTCA CGCAAAGAAT 
GCGTGTAAGA AAATCCACGA CTTCCTTTTC GTGGCATCGA AGATCAAGTC CGTCAGCCTT 
TACAAGAGGG ATGATCGTTG TGCCTACTTG TACGTCCATC ACAACTCAGA TGATTTTCAA 
ATATATTTTC CCTCCACTGA ACGACGACAA AAGTTCTACG ATATGATCCT GGAGATGACT 
GCAGATCAGG TGGTGTTTGT CAATCTCAGC AATGATGACG CTAATATCGA GTACGAGTAC 
GACTACGATG AGCAGAATCG CAAAATGGTG CTGGGCAAAG GTACTTATGG CACCGTGTAC 
GCCGCCAGGG ACAAGCAGAC GCAGGTGCGA ATCGCCATTA AGGAGGTGCC GGAAAAGAAC 
TCGCAGGATG TGCAGCCACT GCACGAGGAG ATTAAGTTAC ACTCTCAGCT GCGACATCGC 
AACATAGTTC GCTATTTGGG ATCGTGCTCG GAGAACGGCT TCTTCAAGAT CTTCATGGAG 
CAGGTGCCGG GAGGATCGCT TTCCGACCTC TTAGAGACCA AATGGGGTCC GCTCAAGGAC 
AACGAGTCCA CCATGGCCTT CTACTCCAAG CAGATCCTCG AGGGGCTGAA GTACCTTCAC 
GAGCAGGACA TTGTTCATCG CGATATAAAG GGTGACAACG TGCTGGTTAA CACGTATAGC 
GGCGTGGTTA AGATCTCCGA TTTCGGCACC TCCAAGCGGC TGGCCCGCAT CAACCCGATG 
ACGGAGACCT TTACGGGCAC CTTGCAGTAC ATGGCGCCGG AGGTGATTGA CCAGGGAGTA 
CGCGGCTATG GCCCGGCGGC TGACATCTGG TCCTTTGGAT GCACAAACGT TGAGATGGCT 
ACGGGCAAAC CACCGTTTAT CGAGCTGGGT AGCGCCCATG CGGCCATGTT CAAGGTGGGC 
TTTTACAAGA AGCATCCGAA CATACCCGAG GAGCTGTCCG CCAACGCAAA GAACTTTATT 
TTGCGTTGCT TTGCCATCAG CGTGATGGAC CGACCCTCCG CCTCGCAACT TCTGGAAGAT 
CCCTTCCTAC AAGATAAACC CCGCAAGGTG CGCCCAGCCC TGCCCATCAA TACTGAGTTC 
GGACGCAGCA TTTCGGTGCC GGCTGATCGG TTTGTGCACA AAACCACACC GCCGCTGAGC 
TACAACACAA CCTGCAATAC GCCCACAACG CCGGAATTGG ACATAACGCA TTCATCCTCG 
GTGGACATTG ACGAGCTGCC CAGCAACCAG TTTATGCTTG AACGGCGAAA TTCCCATGGA 
TTTTTGCTCT CGCCAGAGAT TGAACCTTCC ACGCCGTCCT TGCGTACGAG CATTAGTGAG 
ACGAGCGAGA CTGACGGTTT TTACAGGCTT AAAAAGGATT CGCAGAGGCG GACAACGCTT 
TCCAAGGTGC TGGCACTAGA CGAATCCAAG ATCTGCGATA TTTGGCTGAA GAAGGTCGAC 
GCTGATCAGA ACTCGATTGC GATTAGGAAA AGCGATTTGG AGGTTCTAAT CCGAGGACTG 
CGGGACTACA TTATGAACGA AAACGAAAAG CATTTGGAAG CGACTATCAA CGAACTGAAA 
CAAAAGCTGA ACAACGACGC AGTTGCTCTG GACCATCTTC ATTTGGCGCT GTACTCCTTT 
CAGGACGCCG TGGTCTGCGT ACTGCGGTAC CATTGCATCA AACCCCATTG GATGTTCGCA 
TTGGATAATC TGGTTAAGCG GGCCGTCCAG GCGGCGGTCA CTATTTTCTC TCCTGAACTG 
GGCGCCAATC TGGCGGACAA AGACCTGTCT GGAAATGATG ATGAGAGCTT GCGTACAAGT 
CTAATGCAGA AGGGTTCCAC CGAGAGCCAG GAAAAGCAGA GCCTGGAAAA AGTTCTTCCC 
ACGAGCAACA CTCGCGAAGG TGTGGGATTA ACGCCTGCGG AATCGGGCGG GGATGCTCTG 
TCCAGTTCGA TGGAGTGCGC GCGCATCCTG AGAGACATCC GTGACAATCA GCAGAAGCTG 
CAACGCCAGC TCCTTGAGCA GCAGCGGCAG AGCATGAGGG CCCTGCATAA TATCAGTCAG 
GAGCTGGCTC ACATTTACAT GGGCGGCAGA AAGAGATTAA GACGCACAAT AAACGGCTTT 
AACAAAAAGT GCCATAGAAT GAGTACCAGT GTGGGCGGAA CGTCCGGAGT CCGTCCGAAT 
ATAGCGGGCA ATCCCCGGAG GCAGCTAAAC AAGCATCACT TTGGTCTTCA CGAAGTAGAC 
GAGCAGTTGG AGCAGTGGTT GACCCAGCAG GAAATAGACG AGTTCTCAAA GACGCTCATC 
CTGAATGAAG GTTTTACATT TGACGACTTT ATCTATAACA TGGAAAAGCT GGACCTCATG 
AGGCTGGGTC TGCGCGTTGG CATCGAAGTG CGTTTGTGGA AACTCATTAT ACAAGAACGT 
GTGTGCACCT CGTCCGATTG TGTGGACAGT CCACCAAGCA CTTACCACAA ACCCATTGGA 
GGCAATAGCT CTTTTGCGCT TATTGACAAC AACAATACGC TACCATCACC CACAACCACA 
ACTCCCGGAA CGCCGGGCAT CAAAACGAAA ATCAAGAAAA GCGAAAGCGA GTACGATTCG 
TGCAGCGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477089.3
CDS	206..4297
Translation	MDVVCVMDTAVGDHLEDRQCALEEIKQAVQSAGANFQRVQFERLDFGETNVLETFYNADVAIIDLSILTQQRPLSYHYGVRESFGMKENILTYNDIDSKQTLSLKLSCANYLFLSYKRNAETNSCHLTSQPNSGNNSKEPNAEGRVPTLQWRLKRKLQDVEIQSKAHMREKFLSDMRTARDTYATNGAKLQSILHEMRKRLDDPHVLSGEVVHSFMCSLRDVQDYDAMVRLVNDLKNIPNTRKYVETGNMSFLYAFALNRRNRKGDREKALESSLKALEKKENHFPDMLCLCGRIYKDIFVESDYTDATSLAHAIKWYRQSFEVQPNEYAGINLATLLVIEGKEFTNTEELQHIGMTLNNLIGKKGSLSSLSEYWDVATFFEISVLANDYAKAIQAAECMFKLKPPNWYLKSTIGNISLIHRFRKKPEERQPPIEEQVFQFWMDFFLEATNTEEVKNSIRFPILILEPQKIYMPSYVTINMDADEKSIQIVNICLAHAKNACKKIHDFLFVASKIKSVSLYKRDDRCAYLYVHHNSDDFQIYFPSTERRQKFYDMILEMTADQVVFVNLSNDDANIEYEYDYDEQNRKMVLGKGTYGTVYAARDKQTQVRIAIKEVPEKNSQDVQPLHEEIKLHSQLRHRNIVRYLGSCSENGFFKIFMEQVPGGSLSDLLETKWGPLKDNESTMAFYSKQILEGLKYLHEQDIVHRDIKGDNVLVNTYSGVVKISDFGTSKRLARINPMTETFTGTLQYMAPEVIDQGVRGYGPAADIWSFGCTNVEMATGKPPFIELGSAHAAMFKVGFYKKHPNIPEELSANAKNFILRCFAISVMDRPSASQLLEDPFLQDKPRKVRPALPINTEFGRSISVPADRFVHKTTPPLSYNTTCNTPTTPELDITHSSSVDIDELPSNQFMLERRNSHGFLLSPEIEPSTPSLRTSISETSETDGFYRLKKDSQRRTTLSKVLALDESKICDIWLKKVDADQNSIAIRKSDLEVLIRGLRDYIMNENEKHLEATINELKQKLNNDAVALDHLHLALYSFQDAVVCVLRYHCIKPHWMFALDNLVKRAVQAAVTIFSPELGANLADKDLSGNDDESLRTSLMQKGSTESQEKQSLEKVLPTSNTREGVGLTPAESGGDALSSSMECARILRDIRDNQQKLQRQLLEQQRQSMRALHNISQELAHIYMGGRKRLRRTINGFNKKCHRMSTSVGGTSGVRPNIAGNPRRQLNKHHFGLHEVDEQLEQWLTQQEIDEFSKTLILNEGFTFDDFIYNMEKLDLMRLGLRVGIEVRLWKLIIQERVCTSSDCVDSPPSTYHKPIGGNSSFALIDNNNTLPSPTTTTPGTPGIKTKIKKSESEYDSCSE

Target ORF information:

RefSeq Version	NM_057741.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster apoptotic signal-regulating kinase 1, transcript variant C (Ask1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057741.4

ORF Insert Sequence:

ATGGATGTGG TGTGTGTAAT GGATACGGCG GTGGGCGACC ATCTGGAGGA CCGCCAGTGT 
GCGCTGGAGG AGATCAAGCA GGCAGTGCAG TCGGCGGGCG CCAACTTTCA GCGGGTTCAG 
TTCGAGCGCC TGGACTTCGG CGAGACGAAT GTGCTGGAGA CGTTTTACAA CGCCGATGTG 
GCCATTATCG ACCTGAGCAT TCTCACCCAG CAGCGGCCGC TTTCCTATCA CTACGGCGTT 
AGGGAGAGCT TCGGGATGAA GGAGAACATC CTCACCTACA ACGACATCGA TTCCAAGCAG 
ACGCTCAGTC TCAAGCTCTC GTGTGCCAAC TACCTGTTTC TCAGCTACAA GCGGAATGCG 
GAGACCAATT CCTGCCACCT GACCAGCCAG CCCAACAGCG GCAACAACAG CAAGGAACCC 
AATGCCGAAG GACGAGTGCC CACTTTGCAG TGGCGCCTCA AGCGAAAGCT GCAAGATGTT 
GAGATCCAGT CGAAAGCCCA CATGCGTGAG AAATTTCTGA GTGACATGCG AACGGCGCGG 
GACACGTACG CCACCAACGG CGCCAAGCTG CAGAGTATAT TGCACGAGAT GCGAAAGCGG 
CTGGACGATC CGCATGTCCT GTCTGGCGAA GTGGTACACA GTTTCATGTG CTCGCTGAGG 
GATGTGCAGG ACTACGATGC CATGGTGCGA CTGGTCAATG ACCTGAAGAA CATACCCAAC 
ACCCGGAAGT ACGTGGAGAC GGGCAACATG AGTTTCCTGT ACGCCTTCGC CCTGAACCGA 
AGAAATCGAA AGGGAGACCG TGAAAAGGCT CTGGAATCTT CGCTGAAGGC GCTGGAAAAG 
AAGGAGAACC ACTTTCCGGA CATGCTCTGC CTGTGCGGCC GCATCTACAA GGACATCTTT 
GTGGAATCCG ACTACACGGA CGCCACCAGT TTGGCGCACG CCATCAAGTG GTACCGCCAA 
AGCTTTGAGG TGCAGCCGAA TGAGTATGCA GGCATCAATC TGGCTACTCT GCTGGTTATC 
GAGGGCAAGG AGTTTACCAA TACGGAGGAG TTGCAGCACA TCGGCATGAC CTTAAACAAT 
TTAATCGGTA AAAAGGGCAG CCTATCCTCG CTGAGTGAGT ATTGGGATGT GGCCACCTTC 
TTTGAGATCT CCGTTTTGGC CAACGACTAC GCCAAGGCGA TTCAAGCTGC CGAGTGCATG 
TTCAAGCTAA AGCCACCCAA CTGGTATCTG AAGTCGACCA TCGGAAATAT CTCTCTTATA 
CATCGATTTC GAAAGAAGCC CGAGGAACGC CAGCCGCCCA TCGAGGAGCA GGTGTTCCAG 
TTCTGGATGG ACTTCTTTCT GGAGGCCACC AACACCGAGG AGGTTAAGAA CAGCATTCGC 
TTTCCGATTC TGATCCTCGA GCCTCAGAAG ATTTATATGC CCAGTTATGT GACTATAAAC 
ATGGATGCGG ATGAGAAGTC CATTCAGATT GTGAACATCT GTTTGGCTCA CGCAAAGAAT 
GCGTGTAAGA AAATCCACGA CTTCCTTTTC GTGGCATCGA AGATCAAGTC CGTCAGCCTT 
TACAAGAGGG ATGATCGTTG TGCCTACTTG TACGTCCATC ACAACTCAGA TGATTTTCAA 
ATATATTTTC CCTCCACTGA ACGACGACAA AAGTTCTACG ATATGATCCT GGAGATGACT 
GCAGATCAGG TGGTGTTTGT CAATCTCAGC AATGATGACG CTAATATCGA GTACGAGTAC 
GACTACGATG AGCAGAATCG CAAAATGGTG CTGGGCAAAG GTACTTATGG CACCGTGTAC 
GCCGCCAGGG ACAAGCAGAC GCAGGTGCGA ATCGCCATTA AGGAGGTGCC GGAAAAGAAC 
TCGCAGGATG TGCAGCCACT GCACGAGGAG ATTAAGTTAC ACTCTCAGCT GCGACATCGC 
AACATAGTTC GCTATTTGGG ATCGTGCTCG GAGAACGGCT TCTTCAAGAT CTTCATGGAG 
CAGGTGCCGG GAGGATCGCT TTCCGACCTC TTAGAGACCA AATGGGGTCC GCTCAAGGAC 
AACGAGTCCA CCATGGCCTT CTACTCCAAG CAGATCCTCG AGGGGCTGAA GTACCTTCAC 
GAGCAGGACA TTGTTCATCG CGATATAAAG GGTGACAACG TGCTGGTTAA CACGTATAGC 
GGCGTGGTTA AGATCTCCGA TTTCGGCACC TCCAAGCGGC TGGCCCGCAT CAACCCGATG 
ACGGAGACCT TTACGGGCAC CTTGCAGTAC ATGGCGCCGG AGGTGATTGA CCAGGGAGTA 
CGCGGCTATG GCCCGGCGGC TGACATCTGG TCCTTTGGAT GCACAAACGT TGAGATGGCT 
ACGGGCAAAC CACCGTTTAT CGAGCTGGGT AGCGCCCATG CGGCCATGTT CAAGGTGGGC 
TTTTACAAGA AGCATCCGAA CATACCCGAG GAGCTGTCCG CCAACGCAAA GAACTTTATT 
TTGCGTTGCT TTGCCATCAG CGTGATGGAC CGACCCTCCG CCTCGCAACT TCTGGAAGAT 
CCCTTCCTAC AAGATAAACC CCGCAAGGTG CGCCCAGCCC TGCCCATCAA TACTGAGTTC 
GGACGCAGCA TTTCGGTGCC GGCTGATCGG TTTGTGCACA AAACCACACC GCCGCTGAGC 
TACAACACAA CCTGCAATAC GCCCACAACG CCGGAATTGG ACATAACGCA TTCATCCTCG 
GTGGACATTG ACGAGCTGCC CAGCAACCAG TTTATGCTTG AACGGCGAAA TTCCCATGGA 
TTTTTGCTCT CGCCAGAGAT TGAACCTTCC ACGCCGTCCT TGCGTACGAG CATTAGTGAG 
ACGAGCGAGA CTGACGGTTT TTACAGGCTT AAAAAGGATT CGCAGAGGCG GACAACGCTT 
TCCAAGGTGC TGGCACTAGA CGAATCCAAG ATCTGCGATA TTTGGCTGAA GAAGGTCGAC 
GCTGATCAGA ACTCGATTGC GATTAGGAAA AGCGATTTGG AGGTTCTAAT CCGAGGACTG 
CGGGACTACA TTATGAACGA AAACGAAAAG CATTTGGAAG CGACTATCAA CGAACTGAAA 
CAAAAGCTGA ACAACGACGC AGTTGCTCTG GACCATCTTC ATTTGGCGCT GTACTCCTTT 
CAGGACGCCG TGGTCTGCGT ACTGCGGTAC CATTGCATCA AACCCCATTG GATGTTCGCA 
TTGGATAATC TGGTTAAGCG GGCCGTCCAG GCGGCGGTCA CTATTTTCTC TCCTGAACTG 
GGCGCCAATC TGGCGGACAA AGACCTGTCT GGAAATGATG ATGAGAGCTT GCGTACAAGT 
CTAATGCAGA AGGGTTCCAC CGAGAGCCAG GAAAAGCAGA GCCTGGAAAA AGTTCTTCCC 
ACGAGCAACA CTCGCGAAGG TGTGGGATTA ACGCCTGCGG AATCGGGCGG GGATGCTCTG 
TCCAGTTCGA TGGAGTGCGC GCGCATCCTG AGAGACATCC GTGACAATCA GCAGAAGCTG 
CAACGCCAGC TCCTTGAGCA GCAGCGGCAG AGCATGAGGG CCCTGCATAA TATCAGTCAG 
GAGCTGGCTC ACATTTACAT GGGCGGCAGA AAGAGATTAA GACGCACAAT AAACGGCTTT 
AACAAAAAGT GCCATAGAAT GAGTACCAGT GTGGGCGGAA CGTCCGGAGT CCGTCCGAAT 
ATAGCGGGCA ATCCCCGGAG GCAGCTAAAC AAGCATCACT TTGGTCTTCA CGAAGTAGAC 
GAGCAGTTGG AGCAGTGGTT GACCCAGCAG GAAATAGACG AGTTCTCAAA GACGCTCATC 
CTGAATGAAG GTTTTACATT TGACGACTTT ATCTATAACA TGGAAAAGCT GGACCTCATG 
AGGCTGGGTC TGCGCGTTGG CATCGAAGTG CGTTTGTGGA AACTCATTAT ACAAGAACGT 
GTGTGCACCT CGTCCGATTG TGTGGACAGT CCACCAAGCA CTTACCACAA ACCCATTGGA 
GGCAATAGCT CTTTTGCGCT TATTGACAAC AACAATACGC TACCATCACC CACAACCACA 
ACTCCCGGAA CGCCGGGCAT CAAAACGAAA ATCAAGAAAA GCGAAAGCGA GTACGATTCG 
TGCAGCGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ask1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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