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CYP46A1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CYP46A1
Entrez Gene ID 10858
Full Name cytochrome P450, family 46, subfamily A, polypeptide 1
Synonyms CP46, CYP46
General protein information
Preferred Names
cholesterol 24-hydroxylase
Names
cholesterol 24-hydroxylase
CH24H
cytochrome P450 46A1
cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase)
NP_006659.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

14

14q32.1

Summary This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

604087


mRNA and Protein(s)

mRNA Protein Name
XM_011536364 XP_011534666 cholesterol 24-hydroxylase isoform X1
XM_005267274 XP_005267331 cholesterol 24-hydroxylase isoform X2
XM_011536365 XP_011534667 cholesterol 24-hydroxylase isoform X3
NM_006668 NP_006659 cholesterol 24-hydroxylase precursor



Homo sapiens (human) CYP46A1 NP_006659.1
Pan troglodytes (chimpanzee) CYP46A1 XP_510158.3
Macaca mulatta (Rhesus monkey) LOC705843 XP_001103455.2
Canis lupus familiaris (dog) CYP46A1 XP_005623990.1
Bos taurus (cattle) CYP46A1 NP_001070278.1
Mus musculus (house mouse) Cyp46a1 NP_034140.1
Rattus norvegicus (Norway rat) Cyp46a1 NP_001102193.1
Gallus gallus (chicken) CYP46A1 NP_001238956.1
Arabidopsis thaliana (thale cress) CYP97A3 NP_564384.1
Xenopus (Silurana) tropicalis (western clawed frog) mgc108213 NP_001027517.1
Xenopus (Silurana) tropicalis (western clawed frog) mgc108077 NP_001027519.1
Xenopus (Silurana) tropicalis (western clawed frog) cyp46a1 NP_001072550.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CYP46A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYP46A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu64417 XM_011536364 PREDICTED: Homo sapiens cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu47482 XM_005267274 PREDICTED: Homo sapiens cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu64418 XM_011536365 PREDICTED: Homo sapiens cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu18783 NM_006668 Homo sapiens cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $293.30
$419.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu64417
Clone ID Related Accession (Same CDS sequence) XM_011536364
Accession Version XM_011536364.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1473bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cholesterol 24-hydroxylase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_026437.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCCCCG GGCTGCTGCT GCTCGGCAGC GCCGTCCTGC TCGCCTTCGG CCTCTGCTGC 
ACCTTCGTGC ACCGCGCTCG CAGCCGCTAC GAGCACATCC CCGGGCCGCC GCGGCCCAGT
TTCCTTCTAG GACACCTCCC CTGCTTTTGG AAAAAGGATG AGGTTGGTGG CCGTGTGCTC
CAAGATGTGT TTTTGGATTG GGCTAAGAAG TATGGACCTG TTGTGCGGGT CAACGTCTTC
CACAAAACCT CAGTCATCGT CACGAGTCCT GAGTCGGTTA AGAAGTTCCT GATGTCAACC
AAGTACAACA AGGACTCCAA GATGTACCGT GCGCTCCAGA CTGTGTTTGG TGAGAGACTC
TTCGGCCAAG GCTTGGTGTC CGAATGCAAC TATGAGCGCT GGCACAAGCA GCGGAGAGTC
ATAGACCTGG CCTTCAGCCG GAGCTCCTTG GTTAGCTTAA TGGAAACATT CAACGAGAAG
GCTGAGCAGC TGGTGGAGAT TCTAGAAGCC AAGGCAGATG GGCAGACCCC AGTGTCCATG
CAGGACATGC TGACCTACAC CGCCATGGAC ATCCTGGCCA AGGCAGCTTT TGGGATGGAG
ACCAGTATGC TGCTGGGTGC CCAGAAGCCT CTGTCCCAGG CAGTGAAACT TATGTTGGAG
GGAATCACTG CGTCCCGCAA CACTCTGGCA AAGTTCCTGC CAGGGAAGAG GAAGCAGCTC
CGGGAGGTCC GGGAGAGCAT TCGCTTCCTG CGCCAGGTGG GCAGGGACTG GGTCCAGCGC
CGCCGGGAAG CCCTGAAGAG GGGCGAGGAG GTTCCTGCCG ACATCCTCAC ACAGATTCTG
AAAGCTGAAG AGGGAGCCCA GGACGACGAG GGTCTGCTGG ACAACTTCGT CACCTTCTTC
ATTGCTGGTC ACGAGACCTC TGCCAACCAC TTGGCGTTCA CAGTGATGGA GCTGTCTCGC
CAGCCAGAGA TCGTGGCAAG GCTGCAGGCC GAGGTGGATG AGGTCATTGG TTCTAAGAGG
TACCTGGATT TCGAGGACCT GGGGAGACTG CAGTACCTGT CCCAGGTCCT CAAAGAGTCG
CTGAGGCTGT ACCCACCAGC ATGGGGCACC TTTCGCCTGC TGGAAGAGGA GACCTTGATT
GATGGGTTCA GCACCTATGT CATGGGGCGG ATGGACACAT ACTTTGAGGA CCCGCTGACT
TTCAACCCCG ATCGCTTCGG CCCTGGAGCA CCCAAGCCAC GGTTCACCTA CTTCCCCTTC
TCCCTGGGCC ACCGCTCCTG CATCGGGCAG CAGTTTGCTC AGATGGAGGT GAAGGTGGTC
ATGGCAAAGC TGCTGCAGAG GCTGGAGTTC CGGCTGGTGC CCGGGCAGCG CTTCGGGCTG
CAGGAGCAGG CCACACTCAA GCCACTGGAC CCCGTGCTGT GCACCCTGCG GCCCCGCGGC
TGGCAGCCCG CACCCCCACC ACCCCCCTGC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011534666.1
CDS157..1629
Misc Feature(1)256..1524(+)
Translation

Target ORF information:

RefSeq Version XM_011536364
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011536364

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCCCCG GGCTGCTGCT GCTCGGCAGC GCCGTCCTGC TCGCCTTCGG CCTCTGCTGC 
ACCTTCGTGC ACCGCGCTCG CAGCCGCTAC GAGCACATCC CCGGGCCGCC GCGGCCCAGT
TTCCTTCTAG GACACCTCCC CTGCTTTTGG AAAAAGGATG AGGTTGGTGG CCGTGTGCTC
CAAGATGTGT TTTTGGATTG GGCTAAGAAG TATGGACCTG TTGTGCGGGT CAACGTCTTC
CACAAAACCT CAGTCATCGT CACGAGTCCT GAGTCGGTTA AGAAGTTCCT GATGTCAACC
AAGTACAACA AGGACTCCAA GATGTACCGT GCGCTCCAGA CTGTGTTTGG TGAGAGACTC
TTCGGCCAAG GCTTGGTGTC CGAATGCAAC TATGAGCGCT GGCACAAGCA GCGGAGAGTC
ATAGACCTGG CCTTCAGCCG GAGCTCCTTG GTTAGCTTAA TGGAAACATT CAACGAGAAG
GCTGAGCAGC TGGTGGAGAT TCTAGAAGCC AAGGCAGATG GGCAGACCCC AGTGTCCATG
CAGGACATGC TGACCTACAC CGCCATGGAC ATCCTGGCCA AGGCAGCTTT TGGGATGGAG
ACCAGTATGC TGCTGGGTGC CCAGAAGCCT CTGTCCCAGG CAGTGAAACT TATGTTGGAG
GGAATCACTG CGTCCCGCAA CACTCTGGCA AAGTTCCTGC CAGGGAAGAG GAAGCAGCTC
CGGGAGGTCC GGGAGAGCAT TCGCTTCCTG CGCCAGGTGG GCAGGGACTG GGTCCAGCGC
CGCCGGGAAG CCCTGAAGAG GGGCGAGGAG GTTCCTGCCG ACATCCTCAC ACAGATTCTG
AAAGCTGAAG AGGGAGCCCA GGACGACGAG GGTCTGCTGG ACAACTTCGT CACCTTCTTC
ATTGCTGGTC ACGAGACCTC TGCCAACCAC TTGGCGTTCA CAGTGATGGA GCTGTCTCGC
CAGCCAGAGA TCGTGGCAAG GCTGCAGGCC GAGGTGGATG AGGTCATTGG TTCTAAGAGG
TACCTGGATT TCGAGGACCT GGGGAGACTG CAGTACCTGT CCCAGGTCCT CAAAGAGTCG
CTGAGGCTGT ACCCACCAGC ATGGGGCACC TTTCGCCTGC TGGAAGAGGA GACCTTGATT
GATGGGTTCA GCACCTATGT CATGGGGCGG ATGGACACAT ACTTTGAGGA CCCGCTGACT
TTCAACCCCG ATCGCTTCGG CCCTGGAGCA CCCAAGCCAC GGTTCACCTA CTTCCCCTTC
TCCCTGGGCC ACCGCTCCTG CATCGGGCAG CAGTTTGCTC AGATGGAGGT GAAGGTGGTC
ATGGCAAAGC TGCTGCAGAG GCTGGAGTTC CGGCTGGTGC CCGGGCAGCG CTTCGGGCTG
CAGGAGCAGG CCACACTCAA GCCACTGGAC CCCGTGCTGT GCACCCTGCG GCCCCGCGGC
TGGCAGCCCG CACCCCCACC ACCCCCCTGC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47482
Clone ID Related Accession (Same CDS sequence) XM_005267274
Accession Version XM_005267274.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1068bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cholesterol 24-hydroxylase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_026437.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578825418. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAATGCCT CCTTGGTTAG CTTAATGGAA ACATTCAACG AGAAGGCTGA GCAGCTGGTG 
GAGATTCTAG AAGCCAAGGC AGATGGGCAG ACCCCAGTGT CCATGCAGGA CATGCTGACC
TACACCGCCA TGGACATCCT GGCCAAGGCA GCTTTTGGGA TGGAGACCAG TATGCTGCTG
GGTGCCCAGA AGCCTCTGTC CCAGGCAGTG AAACTTATGT TGGAGGGAAT CACTGCGTCC
CGCAACACTC TGGCAAAGTT CCTGCCAGGG AAGAGGAAGC AGCTCCGGGA GGTCCGGGAG
AGCATTCGCT TCCTGCGCCA GGTGGGCAGG GACTGGGTCC AGCGCCGCCG GGAAGCCCTG
AAGAGGGGCG AGGAGGTTCC TGCCGACATC CTCACACAGA TTCTGAAAGC TGAAGAGGGA
GCCCAGGACG ACGAGGGTCT GCTGGACAAC TTCGTCACCT TCTTCATTGC TGGTCACGAG
ACCTCTGCCA ACCACTTGGC GTTCACAGTG ATGGAGCTGT CTCGCCAGCC AGAGATCGTG
GCAAGGCTGC AGGCCGAGGT GGATGAGGTC ATTGGTTCTA AGAGGTACCT GGATTTCGAG
GACCTGGGGA GACTGCAGTA CCTGTCCCAG GTCCTCAAAG AGTCGCTGAG GCTGTACCCA
CCAGCATGGG GCACCTTTCG CCTGCTGGAA GAGGAGACCT TGATTGATGG GGTCAGAGTC
CCCGGCAACA CCCCGCTCTT GTTCAGCACC TATGTCATGG GGCGGATGGA CACATACTTT
GAGGACCCGC TGACTTTCAA CCCCGATCGC TTCGGCCCTG GAGCACCCAA GCCACGGTTC
ACCTACTTCC CCTTCTCCCT GGGCCACCGC TCCTGCATCG GGCAGCAGTT TGCTCAGATG
GAGGTGAAGG TGGTCATGGC AAAGCTGCTG CAGAGGCTGG AGTTCCGGCT GGTGCCCGGG
CAGCGCTTCG GGCTGCAGGA GCAGGCCACA CTCAAGCCAC TGGACCCCGT GCTGTGCACC
CTGCGGCCCC GCGGCTGGCA GCCCGCACCC CCACCACCCC CCTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005267331.1
CDS72..1139
Misc Feature(1)90..1034(+)
Translation

Target ORF information:

RefSeq Version XM_005267274
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005267274

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAATGCCT CCTTGGTTAG CTTAATGGAA ACATTCAACG AGAAGGCTGA GCAGCTGGTG 
GAGATTCTAG AAGCCAAGGC AGATGGGCAG ACCCCAGTGT CCATGCAGGA CATGCTGACC
TACACCGCCA TGGACATCCT GGCCAAGGCA GCTTTTGGGA TGGAGACCAG TATGCTGCTG
GGTGCCCAGA AGCCTCTGTC CCAGGCAGTG AAACTTATGT TGGAGGGAAT CACTGCGTCC
CGCAACACTC TGGCAAAGTT CCTGCCAGGG AAGAGGAAGC AGCTCCGGGA GGTCCGGGAG
AGCATTCGCT TCCTGCGCCA GGTGGGCAGG GACTGGGTCC AGCGCCGCCG GGAAGCCCTG
AAGAGGGGCG AGGAGGTTCC TGCCGACATC CTCACACAGA TTCTGAAAGC TGAAGAGGGA
GCCCAGGACG ACGAGGGTCT GCTGGACAAC TTCGTCACCT TCTTCATTGC TGGTCACGAG
ACCTCTGCCA ACCACTTGGC GTTCACAGTG ATGGAGCTGT CTCGCCAGCC AGAGATCGTG
GCAAGGCTGC AGGCCGAGGT GGATGAGGTC ATTGGTTCTA AGAGGTACCT GGATTTCGAG
GACCTGGGGA GACTGCAGTA CCTGTCCCAG GTCCTCAAAG AGTCGCTGAG GCTGTACCCA
CCAGCATGGG GCACCTTTCG CCTGCTGGAA GAGGAGACCT TGATTGATGG GGTCAGAGTC
CCCGGCAACA CCCCGCTCTT GTTCAGCACC TATGTCATGG GGCGGATGGA CACATACTTT
GAGGACCCGC TGACTTTCAA CCCCGATCGC TTCGGCCCTG GAGCACCCAA GCCACGGTTC
ACCTACTTCC CCTTCTCCCT GGGCCACCGC TCCTGCATCG GGCAGCAGTT TGCTCAGATG
GAGGTGAAGG TGGTCATGGC AAAGCTGCTG CAGAGGCTGG AGTTCCGGCT GGTGCCCGGG
CAGCGCTTCG GGCTGCAGGA GCAGGCCACA CTCAAGCCAC TGGACCCCGT GCTGTGCACC
CTGCGGCCCC GCGGCTGGCA GCCCGCACCC CCACCACCCC CCTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu64418
Clone ID Related Accession (Same CDS sequence) XM_011536365
Accession Version XM_011536365.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1044bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cholesterol 24-hydroxylase isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_026437.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAAACAT TCAACGAGAA GGCTGAGCAG CTGGTGGAGA TTCTAGAAGC CAAGGCAGAT 
GGGCAGACCC CAGTGTCCAT GCAGGACATG CTGACCTACA CCGCCATGGA CATCCTGGCC
AAGGCAGCTT TTGGGATGGA GACCAGTATG CTGCTGGGTG CCCAGAAGCC TCTGTCCCAG
GCAGTGAAAC TTATGTTGGA GGGAATCACT GCGTCCCGCA ACACTCTGGC AAAGTTCCTG
CCAGGGAAGA GGAAGCAGCT CCGGGAGGTC CGGGAGAGCA TTCGCTTCCT GCGCCAGGTG
GGCAGGGACT GGGTCCAGCG CCGCCGGGAA GCCCTGAAGA GGGGCGAGGA GGTTCCTGCC
GACATCCTCA CACAGATTCT GAAAGCTGAA GAGGGAGCCC AGGACGACGA GGGTCTGCTG
GACAACTTCG TCACCTTCTT CATTGCTGGT CACGAGACCT CTGCCAACCA CTTGGCGTTC
ACAGTGATGG AGCTGTCTCG CCAGCCAGAG ATCGTGGCAA GGCTGCAGGC CGAGGTGGAT
GAGGTCATTG GTTCTAAGAG GTACCTGGAT TTCGAGGACC TGGGGAGACT GCAGTACCTG
TCCCAGGTCC TCAAAGAGTC GCTGAGGCTG TACCCACCAG CATGGGGCAC CTTTCGCCTG
CTGGAAGAGG AGACCTTGAT TGATGGGGTC AGAGTCCCCG GCAACACCCC GCTCTTGTTC
AGCACCTATG TCATGGGGCG GATGGACACA TACTTTGAGG ACCCGCTGAC TTTCAACCCC
GATCGCTTCG GCCCTGGAGC ACCCAAGCCA CGGTTCACCT ACTTCCCCTT CTCCCTGGGC
CACCGCTCCT GCATCGGGCA GCAGTTTGCT CAGATGGAGG TGAAGGTGGT CATGGCAAAG
CTGCTGCAGA GGCTGGAGTT CCGGCTGGTG CCCGGGCAGC GCTTCGGGCT GCAGGAGCAG
GCCACACTCA AGCCACTGGA CCCCGTGCTG TGCACCCTGC GGCCCCGCGG CTGGCAGCCC
GCACCCCCAC CACCCCCCTG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011534667.1
CDS741..1784
Misc Feature(1)744..1679(+)
Translation

Target ORF information:

RefSeq Version XM_011536365
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011536365

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAAACAT TCAACGAGAA GGCTGAGCAG CTGGTGGAGA TTCTAGAAGC CAAGGCAGAT 
GGGCAGACCC CAGTGTCCAT GCAGGACATG CTGACCTACA CCGCCATGGA CATCCTGGCC
AAGGCAGCTT TTGGGATGGA GACCAGTATG CTGCTGGGTG CCCAGAAGCC TCTGTCCCAG
GCAGTGAAAC TTATGTTGGA GGGAATCACT GCGTCCCGCA ACACTCTGGC AAAGTTCCTG
CCAGGGAAGA GGAAGCAGCT CCGGGAGGTC CGGGAGAGCA TTCGCTTCCT GCGCCAGGTG
GGCAGGGACT GGGTCCAGCG CCGCCGGGAA GCCCTGAAGA GGGGCGAGGA GGTTCCTGCC
GACATCCTCA CACAGATTCT GAAAGCTGAA GAGGGAGCCC AGGACGACGA GGGTCTGCTG
GACAACTTCG TCACCTTCTT CATTGCTGGT CACGAGACCT CTGCCAACCA CTTGGCGTTC
ACAGTGATGG AGCTGTCTCG CCAGCCAGAG ATCGTGGCAA GGCTGCAGGC CGAGGTGGAT
GAGGTCATTG GTTCTAAGAG GTACCTGGAT TTCGAGGACC TGGGGAGACT GCAGTACCTG
TCCCAGGTCC TCAAAGAGTC GCTGAGGCTG TACCCACCAG CATGGGGCAC CTTTCGCCTG
CTGGAAGAGG AGACCTTGAT TGATGGGGTC AGAGTCCCCG GCAACACCCC GCTCTTGTTC
AGCACCTATG TCATGGGGCG GATGGACACA TACTTTGAGG ACCCGCTGAC TTTCAACCCC
GATCGCTTCG GCCCTGGAGC ACCCAAGCCA CGGTTCACCT ACTTCCCCTT CTCCCTGGGC
CACCGCTCCT GCATCGGGCA GCAGTTTGCT CAGATGGAGG TGAAGGTGGT CATGGCAAAG
CTGCTGCAGA GGCTGGAGTT CCGGCTGGTG CCCGGGCAGC GCTTCGGGCT GCAGGAGCAG
GCCACACTCA AGCCACTGGA CCCCGTGCTG TGCACCCTGC GGCCCCGCGG CTGGCAGCCC
GCACCCCCAC CACCCCCCTG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18783
Clone ID Related Accession (Same CDS sequence) NM_006668
Accession Version NM_006668.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1503bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cholesterol 24-hydroxylase precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF094480.1. This sequence is a reference standard in the RefSeqGene project. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF094480.1, BC022539.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCCCCG GGCTGCTGCT GCTCGGCAGC GCCGTCCTGC TCGCCTTCGG CCTCTGCTGC 
ACCTTCGTGC ACCGCGCTCG CAGCCGCTAC GAGCACATCC CCGGGCCGCC GCGGCCCAGT
TTCCTTCTAG GACACCTCCC CTGCTTTTGG AAAAAGGATG AGGTTGGTGG CCGTGTGCTC
CAAGATGTGT TTTTGGATTG GGCTAAGAAG TATGGACCTG TTGTGCGGGT CAACGTCTTC
CACAAAACCT CAGTCATCGT CACGAGTCCT GAGTCGGTTA AGAAGTTCCT GATGTCAACC
AAGTACAACA AGGACTCCAA GATGTACCGT GCGCTCCAGA CTGTGTTTGG TGAGAGACTC
TTCGGCCAAG GCTTGGTGTC CGAATGCAAC TATGAGCGCT GGCACAAGCA GCGGAGAGTC
ATAGACCTGG CCTTCAGCCG GAGCTCCTTG GTTAGCTTAA TGGAAACATT CAACGAGAAG
GCTGAGCAGC TGGTGGAGAT TCTAGAAGCC AAGGCAGATG GGCAGACCCC AGTGTCCATG
CAGGACATGC TGACCTACAC CGCCATGGAC ATCCTGGCCA AGGCAGCTTT TGGGATGGAG
ACCAGTATGC TGCTGGGTGC CCAGAAGCCT CTGTCCCAGG CAGTGAAACT TATGTTGGAG
GGAATCACTG CGTCCCGCAA CACTCTGGCA AAGTTCCTGC CAGGGAAGAG GAAGCAGCTC
CGGGAGGTCC GGGAGAGCAT TCGCTTCCTG CGCCAGGTGG GCAGGGACTG GGTCCAGCGC
CGCCGGGAAG CCCTGAAGAG GGGCGAGGAG GTTCCTGCCG ACATCCTCAC ACAGATTCTG
AAAGCTGAAG AGGGAGCCCA GGACGACGAG GGTCTGCTGG ACAACTTCGT CACCTTCTTC
ATTGCTGGTC ACGAGACCTC TGCCAACCAC TTGGCGTTCA CAGTGATGGA GCTGTCTCGC
CAGCCAGAGA TCGTGGCAAG GCTGCAGGCC GAGGTGGATG AGGTCATTGG TTCTAAGAGG
TACCTGGATT TCGAGGACCT GGGGAGACTG CAGTACCTGT CCCAGGTCCT CAAAGAGTCG
CTGAGGCTGT ACCCACCAGC ATGGGGCACC TTTCGCCTGC TGGAAGAGGA GACCTTGATT
GATGGGGTCA GAGTCCCCGG CAACACCCCG CTCTTGTTCA GCACCTATGT CATGGGGCGG
ATGGACACAT ACTTTGAGGA CCCGCTGACT TTCAACCCCG ATCGCTTCGG CCCTGGAGCA
CCCAAGCCAC GGTTCACCTA CTTCCCCTTC TCCCTGGGCC ACCGCTCCTG CATCGGGCAG
CAGTTTGCTC AGATGGAGGT GAAGGTGGTC ATGGCAAAGC TGCTGCAGAG GCTGGAGTTC
CGGCTGGTGC CCGGGCAGCG CTTCGGGCTG CAGGAGCAGG CCACACTCAA GCCACTGGAC
CCCGTGCTGT GCACCCTGCG GCCCCGCGGC TGGCAGCCCG CACCCCCACC ACCCCCCTGC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006659.1
CDS1..1503
Misc Feature(1)7..69(+)
Misc Feature(2)100..1398(+)
Misc Feature(3)1309..1311(+)
Exon (1)1..119
Gene:CYP46A1
Gene Synonym:
Exon (2)120..200
Gene:CYP46A1
Gene Synonym:
Exon (3)201..282
Gene:CYP46A1
Gene Synonym:
Exon (4)283..356
Gene:CYP46A1
Gene Synonym:
Exon (5)357..443
Gene:CYP46A1
Gene Synonym:
Exon (6)444..582
Gene:CYP46A1
Gene Synonym:
Exon (7)583..693
Gene:CYP46A1
Gene Synonym:
Exon (8)694..844
Gene:CYP46A1
Gene Synonym:
Exon (9)845..907
Gene:CYP46A1
Gene Synonym:
Exon (10)908..980
Gene:CYP46A1
Gene Synonym:
Exon (11)981..1065
Gene:CYP46A1
Gene Synonym:
Exon (12)1066..1176
Gene:CYP46A1
Gene Synonym:
Exon (13)1177..1265
Gene:CYP46A1
Gene Synonym:
Exon (14)1266..1332
Gene:CYP46A1
Gene Synonym:
Exon (15)1333..2077
Gene:CYP46A1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_006668
Organism Homo sapiens (human)
Definition Homo sapiens cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006668

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCCCCG GGCTGCTGCT GCTCGGCAGC GCCGTCCTGC TCGCCTTCGG CCTCTGCTGC 
ACCTTCGTGC ACCGCGCTCG CAGCCGCTAC GAGCACATCC CCGGGCCGCC GCGGCCCAGT
TTCCTTCTAG GACACCTCCC CTGCTTTTGG AAAAAGGATG AGGTTGGTGG CCGTGTGCTC
CAAGATGTGT TTTTGGATTG GGCTAAGAAG TATGGACCTG TTGTGCGGGT CAACGTCTTC
CACAAAACCT CAGTCATCGT CACGAGTCCT GAGTCGGTTA AGAAGTTCCT GATGTCAACC
AAGTACAACA AGGACTCCAA GATGTACCGT GCGCTCCAGA CTGTGTTTGG TGAGAGACTC
TTCGGCCAAG GCTTGGTGTC CGAATGCAAC TATGAGCGCT GGCACAAGCA GCGGAGAGTC
ATAGACCTGG CCTTCAGCCG GAGCTCCTTG GTTAGCTTAA TGGAAACATT CAACGAGAAG
GCTGAGCAGC TGGTGGAGAT TCTAGAAGCC AAGGCAGATG GGCAGACCCC AGTGTCCATG
CAGGACATGC TGACCTACAC CGCCATGGAC ATCCTGGCCA AGGCAGCTTT TGGGATGGAG
ACCAGTATGC TGCTGGGTGC CCAGAAGCCT CTGTCCCAGG CAGTGAAACT TATGTTGGAG
GGAATCACTG CGTCCCGCAA CACTCTGGCA AAGTTCCTGC CAGGGAAGAG GAAGCAGCTC
CGGGAGGTCC GGGAGAGCAT TCGCTTCCTG CGCCAGGTGG GCAGGGACTG GGTCCAGCGC
CGCCGGGAAG CCCTGAAGAG GGGCGAGGAG GTTCCTGCCG ACATCCTCAC ACAGATTCTG
AAAGCTGAAG AGGGAGCCCA GGACGACGAG GGTCTGCTGG ACAACTTCGT CACCTTCTTC
ATTGCTGGTC ACGAGACCTC TGCCAACCAC TTGGCGTTCA CAGTGATGGA GCTGTCTCGC
CAGCCAGAGA TCGTGGCAAG GCTGCAGGCC GAGGTGGATG AGGTCATTGG TTCTAAGAGG
TACCTGGATT TCGAGGACCT GGGGAGACTG CAGTACCTGT CCCAGGTCCT CAAAGAGTCG
CTGAGGCTGT ACCCACCAGC ATGGGGCACC TTTCGCCTGC TGGAAGAGGA GACCTTGATT
GATGGGGTCA GAGTCCCCGG CAACACCCCG CTCTTGTTCA GCACCTATGT CATGGGGCGG
ATGGACACAT ACTTTGAGGA CCCGCTGACT TTCAACCCCG ATCGCTTCGG CCCTGGAGCA
CCCAAGCCAC GGTTCACCTA CTTCCCCTTC TCCCTGGGCC ACCGCTCCTG CATCGGGCAG
CAGTTTGCTC AGATGGAGGT GAAGGTGGTC ATGGCAAAGC TGCTGCAGAG GCTGGAGTTC
CGGCTGGTGC CCGGGCAGCG CTTCGGGCTG CAGGAGCAGG CCACACTCAA GCCACTGGAC
CCCGTGCTGT GCACCCTGCG GCCCCGCGGC TGGCAGCCCG CACCCCCACC ACCCCCCTGC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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