×

ADSL cDNA ORF clone, Homo sapiens(Human)

Gene Symbol ADSL
Entrez Gene ID 158
Full Name adenylosuccinate lyase
Synonyms AMPS,ASASE,ASL
General protein information
Preferred Names

adenylosuccinate lyase

Names

adenylosuccinate lyase
adenylosuccinase

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

22

22q13.1

Disorder MIM:

608222

Disorder Html: Adenylosuccinase deficiency, 103050 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000026.2 NP_000017.1 adenylosuccinate lyase isoform a
XM_011529977.2 XP_011528279.1 adenylosuccinate lyase isoform X1
XM_017028637.1 XP_016884126.1 adenylosuccinate lyase isoform X4
XM_017028640.1 XP_016884129.1 adenylosuccinate lyase isoform X8
NM_001123378.2 NP_001116850.1 adenylosuccinate lyase isoform b
XM_017028636.1 XP_016884125.1 adenylosuccinate lyase isoform X2
NM_000026.3 NP_000017.1 adenylosuccinate lyase isoform a
XM_011529980.2 XP_011528282.1 adenylosuccinate lyase isoform X5
XM_017028639.1 XP_016884128.1 adenylosuccinate lyase isoform X7
NM_001317923.1 NP_001304852.1 adenylosuccinate lyase isoform c
XM_011529979.1 XP_011528281.1 adenylosuccinate lyase isoform X3
XM_017028638.1 XP_016884127.1 adenylosuccinate lyase isoform X6



Canis lupus familiaris (dog) ADSL XP_531727.4
Kluyveromyces lactis KLLA0B13189g XP_452119.1
Schizosaccharomyces pombe (fission yeast) ade8 NP_596735.1
Neurospora crassa NCU06187 XP_962999.2
Mus musculus (house mouse) Adsl NP_033764.2
Danio rerio (zebrafish) adsl NP_956193.2
Drosophila melanogaster (fruit fly) CG3590 NP_650586.2
Saccharomyces cerevisiae (baker's yeast) ADE13 NP_013463.1
Eremothecium gossypii AGOS_ABL006C NP_982941.2
Xenopus tropicalis (tropical clawed frog) adsl NP_001005457.1
Magnaporthe oryzae (rice blast fungus) MGG_03645 XP_003716244.1
Homo sapiens (human) ADSL NP_000017.1
Pan troglodytes (chimpanzee) ADSL XP_003317301.1
Bos taurus (cattle) ADSL NP_001095847.1
Gallus gallus (chicken) ADSL NP_990860.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP002378 XP_312577.3
Rattus norvegicus (Norway rat) Adsl NP_001123975.1


Related articles in PubMed

Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism.
Corominas R, Yang X, Lin GN, Kang S, Shen Y, Ghamsari L, Broly M, Rodriguez M, Tam S, Trigg SA, Fan C, Yi S, Tasan M, Lemmens I, Kuang X, Zhao N, Malhotra D, Michaelson JJ, Vacic V, Calderwood MA, Roth FP, Tavernier J, Horvath S, Salehi-Ashtiani K, Korkin D, Sebat J, Hill DE, Hao T, Vidal M, Iakoucheva LM
Nature communications53650(2014 Apr)

Inherent properties of adenylosuccinate lyase could explain S-Ado/SAICAr ratio due to homozygous R426H and R303C mutations.
Ray SP, Duval N, Wilkinson TG 2nd, Shaheen SE, Ghosh K, Patterson D
Biochimica et biophysica acta1834(8)1545-53(2013 Aug)

Structural and biochemical characterization of human adenylosuccinate lyase (ADSL) and the R303C ADSL deficiency-associated mutation.
Ray SP, Deaton MK, Capodagli GC, Calkins LA, Sawle L, Ghosh K, Patterson D, Pegan SD
Biochemistry51(33)6701-13(2012 Aug)

Mutations of ATIC and ADSL affect purinosome assembly in cultured skin fibroblasts from patients with AICA-ribosiduria and ADSL deficiency.
Baresova V, Skopova V, Sikora J, Patterson D, Sovova J, Zikanova M, Kmoch S
Human molecular genetics21(7)1534-43(2012 Apr)

Adenylosuccinate lyase deficiency in the United Kingdom pediatric population: first three cases.
Lundy CT, Jungbluth H, Pohl KR, Siddiqui A, Marinaki AM, Mundy H, Champion MP
Pediatric neurology43(5)351-4(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Missense mutations in the adenylosuccinate lyase is associated with Adenylosuccinate lyase deficiency, an inborn error of purine metabolism characterized by neurological and physiological symptoms.
Title: Inherent properties of adenylosuccinate lyase could explain S-Ado/SAICAr ratio due to homozygous R426H and R303C mutations.

structural and biochemical characterization data of WT and mutant R303C ADSL by enzyme kinetics, product binding by isothermal titration calorimetry and X-ray crystallography to reveal the effects of the R303C mutation that results in a nonparallel reduction in enzyme activity
Title: Structural and biochemical characterization of human adenylosuccinate lyase (ADSL) and the R303C ADSL deficiency-associated mutation.

Results proved in cultured skin fibroblasts from patients with AICA-ribosiduria and ADSL deficiency that various mutations of ADSL destabilize to various degrees purinosome assembly and found that the ability to form purinosomes correlates with clinical phenotypes of individual ADSL patients.
Title: Mutations of ATIC and ADSL affect purinosome assembly in cultured skin fibroblasts from patients with AICA-ribosiduria and ADSL deficiency.

the cases of the only three children diagnosed to date in the United Kingdom with adenylosuccinate lyase deficiency
Title: Adenylosuccinate lyase deficiency in the United Kingdom pediatric population: first three cases.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following ADSL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADSL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18183 NM_000026.3
Latest version!
Homo sapiens adenylosuccinate lyase (ADSL), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu100519 NM_001317923.1
Latest version!
Homo sapiens adenylosuccinate lyase (ADSL), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu17522 NM_001123378.2
Latest version!
Homo sapiens adenylosuccinate lyase (ADSL), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $251.30
$359.00
OHu56198 XM_011529977.2
Latest version!
Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu56200 XM_011529979.1
Latest version!
Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu56201 XM_011529980.2
Latest version!
Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu93396 XM_017028637.1
Latest version!
Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OHu93397 XM_017028638.1
Latest version!
Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OHu93398 XM_017028639.1
Latest version!
Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
OHu93399 XM_017028640.1
Latest version!
Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
OHu93395 XM_017028636.1
Latest version!
Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OHu18183 NM_000026.2 Homo sapiens adenylosuccinate lyase (ADSL), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18183
Clone ID Related Accession (Same CDS sequence) NM_000026.2 , NM_000026.3
Accession Version NM_000026.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1455bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-15
Organism Homo sapiens(Human)
Product adenylosuccinate lyase isoform a
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGATTGCCAT
GAGAAAATCA GAGTGCTTTC TCAGCAGGCA GCTTCTGTGG TTAAGCAGGA AGGGGGTGAC
AATGACCTCA TAGAGCGTAT CCAGGTTGAT GCCTACTTCA GTCCCATTCA CTCCCAGTTG
GATCATTTAC TGGATCCTTC TTCTTTCACT GGTCGTGCCT CCCAGCAGGT GCAGAGATTC
TTAGAAGAGG AGGTGTATCC CCTGTTAAAA CCATATGAAA GCGTGATGAA GGTGAAAGCA
GAATTATGTC TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000017.1
Translation

Target ORF information:

RefSeq Version NM_000026.2
Organism Homo sapiens(Human)
Definition Homo sapiens adenylosuccinate lyase (ADSL), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000026.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGATTGCCAT
GAGAAAATCA GAGTGCTTTC TCAGCAGGCA GCTTCTGTGG TTAAGCAGGA AGGGGGTGAC
AATGACCTCA TAGAGCGTAT CCAGGTTGAT GCCTACTTCA GTCCCATTCA CTCCCAGTTG
GATCATTTAC TGGATCCTTC TTCTTTCACT GGTCGTGCCT CCCAGCAGGT GCAGAGATTC
TTAGAAGAGG AGGTGTATCC CCTGTTAAAA CCATATGAAA GCGTGATGAA GGTGAAAGCA
GAATTATGTC TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18183
Clone ID Related Accession (Same CDS sequence) NM_000026.2 , NM_000026.3
Accession Version NM_000026.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1455bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-10-08
Organism Homo sapiens(Human)
Product adenylosuccinate lyase isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP298407.1, AF067853.1 and BG187609.1. This sequence is a reference standard in the RefSeqGene project. On Dec 14, 2015 this sequence version replaced NM_000026.2. Transcript Variant: This variant (1) represents the predominant transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X65867.1, AF067853.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGATTGCCAT
GAGAAAATCA GAGTGCTTTC TCAGCAGGCA GCTTCTGTGG TTAAGCAGGA AGGGGGTGAC
AATGACCTCA TAGAGCGTAT CCAGGTTGAT GCCTACTTCA GTCCCATTCA CTCCCAGTTG
GATCATTTAC TGGATCCTTC TTCTTTCACT GGTCGTGCCT CCCAGCAGGT GCAGAGATTC
TTAGAAGAGG AGGTGTATCC CCTGTTAAAA CCATATGAAA GCGTGATGAA GGTGAAAGCA
GAATTATGTC TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000017.1
CDS60..1514
Misc Feature(1)63..65
Misc Feature(2)84..86
Misc Feature(3)102..104
Misc Feature(4)108..1439
Misc Feature(5)108..1415
Misc Feature(6)117..122
Misc Feature(7)order(150..155,318..320,345..347,372..377,381..386,528..545,549..551,567..572,579..581,588..593,609..614,660..671,678..680,765..767,771..776,795..797,807..809,828..830,849..851,861..863,867..872,876..881,945..950,963..965,975..977,984..989,993..998,1005..1010,1017..1022,1032..1052,1062..1067,1071..1073,1086..1088,1206..1208,1218..1220,1227..1229,1245..1250,1407..1409,1413..1415)
Misc Feature(8)312..320
Misc Feature(9)order(315..317,378..380,534..536,780..782,942..944,948..950,963..965)
Misc Feature(10)390..395
Misc Feature(11)498..500
Misc Feature(12)942..944
Exon (1)1..212
Exon (2)213..416
Exon (3)417..461
Exon (4)462..541
Exon (5)542..713
Exon (6)714..760
Exon (7)761..851
Exon (8)852..921
Exon (9)922..1069
Exon (10)1070..1160
Exon (11)1161..1250
Exon (12)1251..1427
Exon (13)1428..1740
Translation

Target ORF information:

RefSeq Version NM_000026.3
Organism Homo sapiens(Human)
Definition Homo sapiens adenylosuccinate lyase (ADSL), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000026.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGATTGCCAT
GAGAAAATCA GAGTGCTTTC TCAGCAGGCA GCTTCTGTGG TTAAGCAGGA AGGGGGTGAC
AATGACCTCA TAGAGCGTAT CCAGGTTGAT GCCTACTTCA GTCCCATTCA CTCCCAGTTG
GATCATTTAC TGGATCCTTC TTCTTTCACT GGTCGTGCCT CCCAGCAGGT GCAGAGATTC
TTAGAAGAGG AGGTGTATCC CCTGTTAAAA CCATATGAAA GCGTGATGAA GGTGAAAGCA
GAATTATGTC TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu100519
Clone ID Related Accession (Same CDS sequence) NM_001317923.1
Accession Version NM_001317923.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1263bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-10-08
Organism Homo sapiens(Human)
Product adenylosuccinate lyase isoform c
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP298407.1, KJ535056.1, AF067853.1 and BG187609.1. Transcript Variant: This variant (3) uses an alternate donor splice site at the 5' terminal exon, which results in translation initiation from an in-frame, downstream start codon compared to variant 1. The encoded isoform (c) has a shorter N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KJ535056.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAAATCAA ACCTGGAGAA CATCGACTTC AAGATGGCAG CTGAGGAAGA GAAACGTTTA 
CGACATGATG TGATGGCTCA CGTGCACACA TTTGGCCACT GCTGTCCAAA AGCTGCAGGC
ATTATTCACC TTGGTGCTAC TTCTTGCTAT GTTGGAGACA ATACTGACTT GATTATTCTT
AGAAATGCAC TTGACCTGCT TTTGCCAAAG CTTGCCAGAG TGATCTCTCG GCTTGCCGAC
TTTGCTAAGG AACGAGCCAG TCTACCCACA TTAGGTTTCA CACATTTCCA GCCTGCACAG
CTGACCACAG TTGGGAAACG TTGCTGTCTT TGGATTCAGG ATCTTTGCAT GGATCTCCAG
AACTTGAAGC GTGTCCGAGA TGACCTGCGC TTCCGGGGAG TAAAGGGTAC CACTGGCACT
CAGGCCAGTT TCCTGCAGCT CTTTGAGGGA GATGACCATA AGGTAGAGCA GCTTGACAAG
ATGGTGACAG AAAAGGCAGG ATTTAAGAGA GCTTTCATCA TCACAGGGCA GACATATACA
CGAAAAGTGG ATATTGAAGT ACTGTCTGTG CTGGCTAGCT TGGGGGCATC AGTGCACAAG
ATTTGCACCG ACATACGCCT CCTGGCAAAC CTCAAGGAGA TGGAGGAACC CTTTGAAAAA
CAGCAGATTG GCTCAAGTGC GATGCCATAT AAGCGGAATC CCATGCGTTC AGAACGTTGC
TGCAGTCTTG CCCGCCACCT GATGACCCTT GTCATGGACC CGCTACAGAC AGCATCTGTC
CAGTGGTTTG AACGCACACT GGATGATAGT GCCAACCGAC GGATCTGTTT GGCCGAGGCA
TTTCTTACCG CAGATACTAT ATTGAATACG CTGCAGAACA TTTCTGAAGG ATTGGTCGTG
TACCCCAAAG TAATTGAACG GCGCATTCGG CAAGAGCTGC CTTTCATGGC CACAGAGAAC
ATCATCATGG CCATGGTCAA AGCTGGAGGT AGCCGCCAGG ATTGCCATGA GAAAATCAGA
GTGCTTTCTC AGCAGGCAGC TTCTGTGGTT AAGCAGGAAG GGGGTGACAA TGACCTCATA
GAGCGTATCC AGGTTGATGC CTACTTCAGT CCCATTCACT CCCAGTTGGA TCATTTACTG
GATCCTTCTT CTTTCACTGG TCGTGCCTCC CAGCAGGTGC AGAGATTCTT AGAAGAGGAG
GTGTATCCCC TGTTAAAACC ATATGAAAGC GTGATGAAGG TGAAAGCAGA ATTATGTCTG
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001304852.1
CDS197..1459
Misc Feature(1)149..151
Misc Feature(2)197..1360
Misc Feature(3)order(260..262,323..325,479..481,725..727,887..889,893..895,908..910)
Misc Feature(4)order(263..265,290..292,317..322,326..331,473..490,494..496,512..517,524..526,533..538,554..559,605..616,623..625,710..712,716..721,740..742,752..754,773..775,794..796,806..808,812..817,821..826,890..895,908..910,920..922,929..934,938..943,950..955,962..967,977..997,1007..1012,1016..1018,1031..1033,1151..1153,1163..1165,1172..1174,1190..1195,1352..1354,1358..1360)
Exon (1)1..157
Exon (2)158..361
Exon (3)362..406
Exon (4)407..486
Exon (5)487..658
Exon (6)659..705
Exon (7)706..796
Exon (8)797..866
Exon (9)867..1014
Exon (10)1015..1105
Exon (11)1106..1195
Exon (12)1196..1372
Exon (13)1373..1685
Translation

Target ORF information:

RefSeq Version NM_001317923.1
Organism Homo sapiens(Human)
Definition Homo sapiens adenylosuccinate lyase (ADSL), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001317923.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAAATCAA ACCTGGAGAA CATCGACTTC AAGATGGCAG CTGAGGAAGA GAAACGTTTA 
CGACATGATG TGATGGCTCA CGTGCACACA TTTGGCCACT GCTGTCCAAA AGCTGCAGGC
ATTATTCACC TTGGTGCTAC TTCTTGCTAT GTTGGAGACA ATACTGACTT GATTATTCTT
AGAAATGCAC TTGACCTGCT TTTGCCAAAG CTTGCCAGAG TGATCTCTCG GCTTGCCGAC
TTTGCTAAGG AACGAGCCAG TCTACCCACA TTAGGTTTCA CACATTTCCA GCCTGCACAG
CTGACCACAG TTGGGAAACG TTGCTGTCTT TGGATTCAGG ATCTTTGCAT GGATCTCCAG
AACTTGAAGC GTGTCCGAGA TGACCTGCGC TTCCGGGGAG TAAAGGGTAC CACTGGCACT
CAGGCCAGTT TCCTGCAGCT CTTTGAGGGA GATGACCATA AGGTAGAGCA GCTTGACAAG
ATGGTGACAG AAAAGGCAGG ATTTAAGAGA GCTTTCATCA TCACAGGGCA GACATATACA
CGAAAAGTGG ATATTGAAGT ACTGTCTGTG CTGGCTAGCT TGGGGGCATC AGTGCACAAG
ATTTGCACCG ACATACGCCT CCTGGCAAAC CTCAAGGAGA TGGAGGAACC CTTTGAAAAA
CAGCAGATTG GCTCAAGTGC GATGCCATAT AAGCGGAATC CCATGCGTTC AGAACGTTGC
TGCAGTCTTG CCCGCCACCT GATGACCCTT GTCATGGACC CGCTACAGAC AGCATCTGTC
CAGTGGTTTG AACGCACACT GGATGATAGT GCCAACCGAC GGATCTGTTT GGCCGAGGCA
TTTCTTACCG CAGATACTAT ATTGAATACG CTGCAGAACA TTTCTGAAGG ATTGGTCGTG
TACCCCAAAG TAATTGAACG GCGCATTCGG CAAGAGCTGC CTTTCATGGC CACAGAGAAC
ATCATCATGG CCATGGTCAA AGCTGGAGGT AGCCGCCAGG ATTGCCATGA GAAAATCAGA
GTGCTTTCTC AGCAGGCAGC TTCTGTGGTT AAGCAGGAAG GGGGTGACAA TGACCTCATA
GAGCGTATCC AGGTTGATGC CTACTTCAGT CCCATTCACT CCCAGTTGGA TCATTTACTG
GATCCTTCTT CTTTCACTGG TCGTGCCTCC CAGCAGGTGC AGAGATTCTT AGAAGAGGAG
GTGTATCCCC TGTTAAAACC ATATGAAAGC GTGATGAAGG TGAAAGCAGA ATTATGTCTG
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17522
Clone ID Related Accession (Same CDS sequence) NM_001123378.2
Accession Version NM_001123378.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1278bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-10-08
Organism Homo sapiens(Human)
Product adenylosuccinate lyase isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP298407.1, AF067854.1 and BG187609.1. On Dec 14, 2015 this sequence version replaced NM_001123378.1. Transcript Variant: This variant (2) lacks the penultimate, in-frame coding exon compared to variant 1. The resulting isoform (b) is shorter, missing an internal protein segment compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF067854.1, AK225042.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGTGCAGAGA
TTCTTAGAAG AGGAGGTGTA TCCCCTGTTA AAACCATATG AAAGCGTGAT GAAGGTGAAA
GCAGAATTAT GTCTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001116850.1
CDS60..1337
Misc Feature(1)63..65
Misc Feature(2)84..86
Misc Feature(3)102..104
Misc Feature(4)108..1250
Misc Feature(5)117..122
Misc Feature(6)order(150..155,318..320,345..347,372..377,381..386,528..545,549..551,567..572,579..581,588..593,609..614,660..671,678..680,765..767,771..776,795..797,807..809,828..830,849..851,861..863,867..872,876..881,945..950,963..965,975..977,984..989,993..998,1005..1010,1017..1022,1032..1052,1062..1067,1071..1073,1086..1088,1206..1208,1218..1220,1227..1229,1245..1250)
Misc Feature(7)312..320
Misc Feature(8)order(315..317,378..380,534..536,780..782,942..944,948..950,963..965)
Misc Feature(9)390..395
Misc Feature(10)498..500
Misc Feature(11)942..944
Exon (1)1..212
Exon (2)213..416
Exon (3)417..461
Exon (4)462..541
Exon (5)542..713
Exon (6)714..760
Exon (7)761..851
Exon (8)852..921
Exon (9)922..1069
Exon (10)1070..1160
Exon (11)1161..1250
Exon (12)1251..1563
Translation

Target ORF information:

RefSeq Version NM_001123378.2
Organism Homo sapiens(Human)
Definition Homo sapiens adenylosuccinate lyase (ADSL), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001123378.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGTGCAGAGA
TTCTTAGAAG AGGAGGTGTA TCCCCTGTTA AAACCATATG AAAGCGTGAT GAAGGTGAAA
GCAGAATTAT GTCTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56198
Clone ID Related Accession (Same CDS sequence) XM_011529977.2
Accession Version XM_011529977.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1581bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product adenylosuccinate lyase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011529977.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGATTGCCAT
GAGAAAATCA GAGTGCTTTC TCAGCAGGCA GCTTCTGTGG TTAAGCAGGA AGGGGGTGAC
AATGACCTCA TAGAGCGTAT CCAGGTTGAT GCCTACTTCA GTCCCATTCA CTCCCAGTTG
GATCATTTAC TGGATCCTTC TTCTTTCACT GGTCGTGCCT CCCAGCAGGT GCAGAGATTC
TTAGAAGAGG AGGTGTATCC CCTGTTAAAA CCATATGAAA GCGTGATGAA GTGTAAGCAA
GTGCCATTGG ACAAGAACGT TGTCTGTGTC ATTCCTTGTT GTATTTCCAA TAGCAAGAGC
ACATCAGATA CTTGGCAACT AGGCAGTCTT CCAGCTTCTG CCACTGCGCC CGACTTCCTT
GTTGCATGGA TTTATAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528279.1
CDS60..1640
Misc Feature(1)108..1439
Misc Feature(2)108..1415
Misc Feature(3)order(150..155,318..320,345..347,372..377,381..386,528..545,549..551,567..572,579..581,588..593,609..614,660..671,678..680,765..767,771..776,795..797,807..809,828..830,849..851,861..863,867..872,876..881,945..950,963..965,975..977,984..989,993..998,1005..1010,1017..1022,1032..1052,1062..1067,1071..1073,1086..1088,1206..1208,1218..1220,1227..1229,1245..1250,1407..1409,1413..1415)
Misc Feature(4)order(315..317,378..380,534..536,780..782,942..944,948..950,963..965)
Translation

Target ORF information:

RefSeq Version XM_011529977.2
Organism Homo sapiens(Human)
Definition Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529977.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGATTGCCAT
GAGAAAATCA GAGTGCTTTC TCAGCAGGCA GCTTCTGTGG TTAAGCAGGA AGGGGGTGAC
AATGACCTCA TAGAGCGTAT CCAGGTTGAT GCCTACTTCA GTCCCATTCA CTCCCAGTTG
GATCATTTAC TGGATCCTTC TTCTTTCACT GGTCGTGCCT CCCAGCAGGT GCAGAGATTC
TTAGAAGAGG AGGTGTATCC CCTGTTAAAA CCATATGAAA GCGTGATGAA GTGTAAGCAA
GTGCCATTGG ACAAGAACGT TGTCTGTGTC ATTCCTTGTT GTATTTCCAA TAGCAAGAGC
ACATCAGATA CTTGGCAACT AGGCAGTCTT CCAGCTTCTG CCACTGCGCC CGACTTCCTT
GTTGCATGGA TTTATAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56200
Clone ID Related Accession (Same CDS sequence) XM_011529979.1
Accession Version XM_011529979.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product adenylosuccinate lyase isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGATTGCCAT
GAGAAAATCA GAGTGCTTTC TCAGCAGGCA GCTTCTGTGG TTAAGCAGGA AGGGGGTGAC
AATGACCTCA TAGAGCGTAT CCAGGTTGAT GCCTACTTCA GTCCCATTCA CTCCCAGTTG
GATCATTTAC TGGATCCTTC TTCTTTCACT GGTCGTGCCT CCCAGCAGGT GCAGAGATTC
TTAGAAGAGG AGGTGTATCC CCTGTTAAAA CCATATGAAA GCGTGATGAA GGTTTCCTTC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528281.1
CDS60..1502
Misc Feature(1)108..1439
Misc Feature(2)108..1415
Misc Feature(3)order(150..155,318..320,345..347,372..377,381..386,528..545,549..551,567..572,579..581,588..593,609..614,660..671,678..680,765..767,771..776,795..797,807..809,828..830,849..851,861..863,867..872,876..881,945..950,963..965,975..977,984..989,993..998,1005..1010,1017..1022,1032..1052,1062..1067,1071..1073,1086..1088,1206..1208,1218..1220,1227..1229,1245..1250,1407..1409,1413..1415)
Misc Feature(4)order(315..317,378..380,534..536,780..782,942..944,948..950,963..965)
Translation

Target ORF information:

RefSeq Version XM_011529979.1
Organism Homo sapiens(Human)
Definition Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529979.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGATTGCCAT
GAGAAAATCA GAGTGCTTTC TCAGCAGGCA GCTTCTGTGG TTAAGCAGGA AGGGGGTGAC
AATGACCTCA TAGAGCGTAT CCAGGTTGAT GCCTACTTCA GTCCCATTCA CTCCCAGTTG
GATCATTTAC TGGATCCTTC TTCTTTCACT GGTCGTGCCT CCCAGCAGGT GCAGAGATTC
TTAGAAGAGG AGGTGTATCC CCTGTTAAAA CCATATGAAA GCGTGATGAA GGTTTCCTTC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56201
Clone ID Related Accession (Same CDS sequence) XM_011529980.2
Accession Version XM_011529980.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1404bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product adenylosuccinate lyase isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011529980.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGTGCAGAGA
TTCTTAGAAG AGGAGGTGTA TCCCCTGTTA AAACCATATG AAAGCGTGAT GAAGTGTAAG
CAAGTGCCAT TGGACAAGAA CGTTGTCTGT GTCATTCCTT GTTGTATTTC CAATAGCAAG
AGCACATCAG ATACTTGGCA ACTAGGCAGT CTTCCAGCTT CTGCCACTGC GCCCGACTTC
CTTGTTGCAT GGATTTATAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528282.1
CDS60..1463
Misc Feature(1)108..1250
Misc Feature(2)order(150..155,318..320,345..347,372..377,381..386,528..545,549..551,567..572,579..581,588..593,609..614,660..671,678..680,765..767,771..776,795..797,807..809,828..830,849..851,861..863,867..872,876..881,945..950,963..965,975..977,984..989,993..998,1005..1010,1017..1022,1032..1052,1062..1067,1071..1073,1086..1088,1206..1208,1218..1220,1227..1229,1245..1250)
Misc Feature(3)order(315..317,378..380,534..536,780..782,942..944,948..950,963..965)
Translation

Target ORF information:

RefSeq Version XM_011529980.2
Organism Homo sapiens(Human)
Definition Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529980.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGTGCAGAGA
TTCTTAGAAG AGGAGGTGTA TCCCCTGTTA AAACCATATG AAAGCGTGAT GAAGTGTAAG
CAAGTGCCAT TGGACAAGAA CGTTGTCTGT GTCATTCCTT GTTGTATTTC CAATAGCAAG
AGCACATCAG ATACTTGGCA ACTAGGCAGT CTTCCAGCTT CTGCCACTGC GCCCGACTTC
CTTGTTGCAT GGATTTATAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu93396
Clone ID Related Accession (Same CDS sequence) XM_017028637.1
Accession Version XM_017028637.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1410bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product adenylosuccinate lyase isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTCTT
GCCAGAGTGA TCTCTCGGCT TGCCGACTTT GCTAAGGAAC GAGCCAGTCT ACCCACATTA
GGTTTCACAC ATTTCCAGCC TGCACAGCTG ACCACAGTTG GGAAACGTTG CTGTCTTTGG
ATTCAGGATC TTTGCATGGA TCTCCAGAAC TTGAAGCGTG TCCGAGATGA CCTGCGCTTC
CGGGGAGTAA AGGGTACCAC TGGCACTCAG GCCAGTTTCC TGCAGCTCTT TGAGGGAGAT
GACCATAAGG TAGAGCAGCT TGACAAGATG GTGACAGAAA AGGCAGGATT TAAGAGAGCT
TTCATCATCA CAGGGCAGAC ATATACACGA AAAGTGGATA TTGAAGTACT GTCTGTGCTG
GCTAGCTTGG GGGCATCAGT GCACAAGATT TGCACCGACA TACGCCTCCT GGCAAACCTC
AAGGAGATGG AGGAACCCTT TGAAAAACAG CAGATTGGCT CAAGTGCGAT GCCATATAAG
CGGAATCCCA TGCGTTCAGA ACGTTGCTGC AGTCTTGCCC GCCACCTGAT GACCCTTGTC
ATGGACCCGC TACAGACAGC ATCTGTCCAG TGGTTTGAAC GCACACTGGA TGATAGTGCC
AACCGACGGA TCTGTTTGGC CGAGGCATTT CTTACCGCAG ATACTATATT GAATACGCTG
CAGAACATTT CTGAAGGATT GGTCGTGTAC CCCAAAGTAA TTGAACGGCG CATTCGGCAA
GAGCTGCCTT TCATGGCCAC AGAGAACATC ATCATGGCCA TGGTCAAAGC TGGAGGTAGC
CGCCAGGATT GCCATGAGAA AATCAGAGTG CTTTCTCAGC AGGCAGCTTC TGTGGTTAAG
CAGGAAGGGG GTGACAATGA CCTCATAGAG CGTATCCAGG TTGATGCCTA CTTCAGTCCC
ATTCACTCCC AGTTGGATCA TTTACTGGAT CCTTCTTCTT TCACTGGTCG TGCCTCCCAG
CAGGTGCAGA GATTCTTAGA AGAGGAGGTG TATCCCCTGT TAAAACCATA TGAAAGCGTG
ATGAAGGTGA AAGCAGAATT ATGTCTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016884126.1
CDS25..1434
Translation

Target ORF information:

RefSeq Version XM_017028637.1
Organism Homo sapiens(Human)
Definition Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017028637.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTCTT
GCCAGAGTGA TCTCTCGGCT TGCCGACTTT GCTAAGGAAC GAGCCAGTCT ACCCACATTA
GGTTTCACAC ATTTCCAGCC TGCACAGCTG ACCACAGTTG GGAAACGTTG CTGTCTTTGG
ATTCAGGATC TTTGCATGGA TCTCCAGAAC TTGAAGCGTG TCCGAGATGA CCTGCGCTTC
CGGGGAGTAA AGGGTACCAC TGGCACTCAG GCCAGTTTCC TGCAGCTCTT TGAGGGAGAT
GACCATAAGG TAGAGCAGCT TGACAAGATG GTGACAGAAA AGGCAGGATT TAAGAGAGCT
TTCATCATCA CAGGGCAGAC ATATACACGA AAAGTGGATA TTGAAGTACT GTCTGTGCTG
GCTAGCTTGG GGGCATCAGT GCACAAGATT TGCACCGACA TACGCCTCCT GGCAAACCTC
AAGGAGATGG AGGAACCCTT TGAAAAACAG CAGATTGGCT CAAGTGCGAT GCCATATAAG
CGGAATCCCA TGCGTTCAGA ACGTTGCTGC AGTCTTGCCC GCCACCTGAT GACCCTTGTC
ATGGACCCGC TACAGACAGC ATCTGTCCAG TGGTTTGAAC GCACACTGGA TGATAGTGCC
AACCGACGGA TCTGTTTGGC CGAGGCATTT CTTACCGCAG ATACTATATT GAATACGCTG
CAGAACATTT CTGAAGGATT GGTCGTGTAC CCCAAAGTAA TTGAACGGCG CATTCGGCAA
GAGCTGCCTT TCATGGCCAC AGAGAACATC ATCATGGCCA TGGTCAAAGC TGGAGGTAGC
CGCCAGGATT GCCATGAGAA AATCAGAGTG CTTTCTCAGC AGGCAGCTTC TGTGGTTAAG
CAGGAAGGGG GTGACAATGA CCTCATAGAG CGTATCCAGG TTGATGCCTA CTTCAGTCCC
ATTCACTCCC AGTTGGATCA TTTACTGGAT CCTTCTTCTT TCACTGGTCG TGCCTCCCAG
CAGGTGCAGA GATTCTTAGA AGAGGAGGTG TATCCCCTGT TAAAACCATA TGAAAGCGTG
ATGAAGGTGA AAGCAGAATT ATGTCTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu93397
Clone ID Related Accession (Same CDS sequence) XM_017028638.1
Accession Version XM_017028638.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1116bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product adenylosuccinate lyase isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTTGGAGA CAATACTGCC TGCACAGCTG ACCACAGTTG GGAAACGTTG CTGTCTTTGG 
ATTCAGGATC TTTGCATGGA TCTCCAGAAC TTGAAGCGTG TCCGAGATGA CCTGCGCTTC
CGGGGAGTAA AGGGTACCAC TGGCACTCAG GCCAGTTTCC TGCAGCTCTT TGAGGGAGAT
GACCATAAGG TAGAGCAGCT TGACAAGATG GTGACAGAAA AGGCAGGATT TAAGAGAGCT
TTCATCATCA CAGGGCAGAC ATATACACGA AAAGTGGATA TTGAAGTACT GTCTGTGCTG
GCTAGCTTGG GGGCATCAGT GCACAAGATT TGCACCGACA TACGCCTCCT GGCAAACCTC
AAGGAGATGG AGGAACCCTT TGAAAAACAG CAGATTGGCT CAAGTGCGAT GCCATATAAG
CGGAATCCCA TGCGTTCAGA ACGTTGCTGC AGTCTTGCCC GCCACCTGAT GACCCTTGTC
ATGGACCCGC TACAGACAGC ATCTGTCCAG TGGTTTGAAC GCACACTGGA TGATAGTGCC
AACCGACGGA TCTGTTTGGC CGAGGCATTT CTTACCGCAG ATACTATATT GAATACGCTG
CAGAACATTT CTGAAGGATT GGTCGTGTAC CCCAAAGTAA TTGAACGGCG CATTCGGCAA
GAGCTGCCTT TCATGGCCAC AGAGAACATC ATCATGGCCA TGGTCAAAGC TGGAGGTAGC
CGCCAGGATT GCCATGAGAA AATCAGAGTG CTTTCTCAGC AGGCAGCTTC TGTGGTTAAG
CAGGAAGGGG GTGACAATGA CCTCATAGAG CGTATCCAGG TTGATGCCTA CTTCAGTCCC
ATTCACTCCC AGTTGGATCA TTTACTGGAT CCTTCTTCTT TCACTGGTCG TGCCTCCCAG
CAGGTGCAGA GATTCTTAGA AGAGGAGGTG TATCCCCTGT TAAAACCATA TGAAAGCGTG
ATGAAGTGTA AGCAAGTGCC ATTGGACAAG AACGTTGTCT GTGTCATTCC TTGTTGTATT
TCCAATAGCA AGAGCACATC AGATACTTGG CAACTAGGCA GTCTTCCAGC TTCTGCCACT
GCGCCCGACT TCCTTGTTGC ATGGATTTAT AGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016884127.1
CDS382..1497
Translation

Target ORF information:

RefSeq Version XM_017028638.1
Organism Homo sapiens(Human)
Definition Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017028638.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTTGGAGA CAATACTGCC TGCACAGCTG ACCACAGTTG GGAAACGTTG CTGTCTTTGG 
ATTCAGGATC TTTGCATGGA TCTCCAGAAC TTGAAGCGTG TCCGAGATGA CCTGCGCTTC
CGGGGAGTAA AGGGTACCAC TGGCACTCAG GCCAGTTTCC TGCAGCTCTT TGAGGGAGAT
GACCATAAGG TAGAGCAGCT TGACAAGATG GTGACAGAAA AGGCAGGATT TAAGAGAGCT
TTCATCATCA CAGGGCAGAC ATATACACGA AAAGTGGATA TTGAAGTACT GTCTGTGCTG
GCTAGCTTGG GGGCATCAGT GCACAAGATT TGCACCGACA TACGCCTCCT GGCAAACCTC
AAGGAGATGG AGGAACCCTT TGAAAAACAG CAGATTGGCT CAAGTGCGAT GCCATATAAG
CGGAATCCCA TGCGTTCAGA ACGTTGCTGC AGTCTTGCCC GCCACCTGAT GACCCTTGTC
ATGGACCCGC TACAGACAGC ATCTGTCCAG TGGTTTGAAC GCACACTGGA TGATAGTGCC
AACCGACGGA TCTGTTTGGC CGAGGCATTT CTTACCGCAG ATACTATATT GAATACGCTG
CAGAACATTT CTGAAGGATT GGTCGTGTAC CCCAAAGTAA TTGAACGGCG CATTCGGCAA
GAGCTGCCTT TCATGGCCAC AGAGAACATC ATCATGGCCA TGGTCAAAGC TGGAGGTAGC
CGCCAGGATT GCCATGAGAA AATCAGAGTG CTTTCTCAGC AGGCAGCTTC TGTGGTTAAG
CAGGAAGGGG GTGACAATGA CCTCATAGAG CGTATCCAGG TTGATGCCTA CTTCAGTCCC
ATTCACTCCC AGTTGGATCA TTTACTGGAT CCTTCTTCTT TCACTGGTCG TGCCTCCCAG
CAGGTGCAGA GATTCTTAGA AGAGGAGGTG TATCCCCTGT TAAAACCATA TGAAAGCGTG
ATGAAGTGTA AGCAAGTGCC ATTGGACAAG AACGTTGTCT GTGTCATTCC TTGTTGTATT
TCCAATAGCA AGAGCACATC AGATACTTGG CAACTAGGCA GTCTTCCAGC TTCTGCCACT
GCGCCCGACT TCCTTGTTGC ATGGATTTAT AGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu93398
Clone ID Related Accession (Same CDS sequence) XM_017028639.1
Accession Version XM_017028639.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 990bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product adenylosuccinate lyase isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTTGGAGA CAATACTGCC TGCACAGCTG ACCACAGTTG GGAAACGTTG CTGTCTTTGG 
ATTCAGGATC TTTGCATGGA TCTCCAGAAC TTGAAGCGTG TCCGAGATGA CCTGCGCTTC
CGGGGAGTAA AGGGTACCAC TGGCACTCAG GCCAGTTTCC TGCAGCTCTT TGAGGGAGAT
GACCATAAGG TAGAGCAGCT TGACAAGATG GTGACAGAAA AGGCAGGATT TAAGAGAGCT
TTCATCATCA CAGGGCAGAC ATATACACGA AAAGTGGATA TTGAAGTACT GTCTGTGCTG
GCTAGCTTGG GGGCATCAGT GCACAAGATT TGCACCGACA TACGCCTCCT GGCAAACCTC
AAGGAGATGG AGGAACCCTT TGAAAAACAG CAGATTGGCT CAAGTGCGAT GCCATATAAG
CGGAATCCCA TGCGTTCAGA ACGTTGCTGC AGTCTTGCCC GCCACCTGAT GACCCTTGTC
ATGGACCCGC TACAGACAGC ATCTGTCCAG TGGTTTGAAC GCACACTGGA TGATAGTGCC
AACCGACGGA TCTGTTTGGC CGAGGCATTT CTTACCGCAG ATACTATATT GAATACGCTG
CAGAACATTT CTGAAGGATT GGTCGTGTAC CCCAAAGTAA TTGAACGGCG CATTCGGCAA
GAGCTGCCTT TCATGGCCAC AGAGAACATC ATCATGGCCA TGGTCAAAGC TGGAGGTAGC
CGCCAGGATT GCCATGAGAA AATCAGAGTG CTTTCTCAGC AGGCAGCTTC TGTGGTTAAG
CAGGAAGGGG GTGACAATGA CCTCATAGAG CGTATCCAGG TTGATGCCTA CTTCAGTCCC
ATTCACTCCC AGTTGGATCA TTTACTGGAT CCTTCTTCTT TCACTGGTCG TGCCTCCCAG
CAGGTGCAGA GATTCTTAGA AGAGGAGGTG TATCCCCTGT TAAAACCATA TGAAAGCGTG
ATGAAGGTGA AAGCAGAATT ATGTCTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016884128.1
CDS382..1371
Translation

Target ORF information:

RefSeq Version XM_017028639.1
Organism Homo sapiens(Human)
Definition Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017028639.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTTGGAGA CAATACTGCC TGCACAGCTG ACCACAGTTG GGAAACGTTG CTGTCTTTGG 
ATTCAGGATC TTTGCATGGA TCTCCAGAAC TTGAAGCGTG TCCGAGATGA CCTGCGCTTC
CGGGGAGTAA AGGGTACCAC TGGCACTCAG GCCAGTTTCC TGCAGCTCTT TGAGGGAGAT
GACCATAAGG TAGAGCAGCT TGACAAGATG GTGACAGAAA AGGCAGGATT TAAGAGAGCT
TTCATCATCA CAGGGCAGAC ATATACACGA AAAGTGGATA TTGAAGTACT GTCTGTGCTG
GCTAGCTTGG GGGCATCAGT GCACAAGATT TGCACCGACA TACGCCTCCT GGCAAACCTC
AAGGAGATGG AGGAACCCTT TGAAAAACAG CAGATTGGCT CAAGTGCGAT GCCATATAAG
CGGAATCCCA TGCGTTCAGA ACGTTGCTGC AGTCTTGCCC GCCACCTGAT GACCCTTGTC
ATGGACCCGC TACAGACAGC ATCTGTCCAG TGGTTTGAAC GCACACTGGA TGATAGTGCC
AACCGACGGA TCTGTTTGGC CGAGGCATTT CTTACCGCAG ATACTATATT GAATACGCTG
CAGAACATTT CTGAAGGATT GGTCGTGTAC CCCAAAGTAA TTGAACGGCG CATTCGGCAA
GAGCTGCCTT TCATGGCCAC AGAGAACATC ATCATGGCCA TGGTCAAAGC TGGAGGTAGC
CGCCAGGATT GCCATGAGAA AATCAGAGTG CTTTCTCAGC AGGCAGCTTC TGTGGTTAAG
CAGGAAGGGG GTGACAATGA CCTCATAGAG CGTATCCAGG TTGATGCCTA CTTCAGTCCC
ATTCACTCCC AGTTGGATCA TTTACTGGAT CCTTCTTCTT TCACTGGTCG TGCCTCCCAG
CAGGTGCAGA GATTCTTAGA AGAGGAGGTG TATCCCCTGT TAAAACCATA TGAAAGCGTG
ATGAAGGTGA AAGCAGAATT ATGTCTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu93399
Clone ID Related Accession (Same CDS sequence) XM_017028640.1
Accession Version XM_017028640.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 750bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product adenylosuccinate lyase isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGGAAC CCTTTGAAAA ACAGCAGATT GGCTCAAGTG CGATGCCATA TAAGCGGAAT 
CCCATGCGTT CAGAACGTTG CTGCAGTCTT GCCCGCCACC TGATGACCCT TGTCATGGAC
CCGCTACAGA CAGCATCTGT CCAGTGGTTT GAACGCACAC TGGATGATAG TGCCAACCGA
CGGATCTGTT TGGCCGAGGC ATTTCTTACC GCAGATACTA TATTGAATAC GCTGCAGAAC
ATTTCTGAAG GATTGGTCGT GTACCCCAAA GTAATTGAAC GGCGCATTCG GCAAGAGCTG
CCTTTCATGG CCACAGAGAA CATCATCATG GCCATGGTCA AAGCTGGAGG TAGCCGCCAG
GATTGCCATG AGAAAATCAG AGTGCTTTCT CAGCAGGCAG CTTCTGTGGT TAAGCAGGAA
GGGGGTGACA ATGACCTCAT AGAGCGTATC CAGGTTGATG CCTACTTCAG TCCCATTCAC
TCCCAGTTGG ATCATTTACT GGATCCTTCT TCTTTCACTG GTCGTGCCTC CCAGCAGGTG
CAGAGATTCT TAGAAGAGGA GGTGTATCCC CTGTTAAAAC CATATGAAAG CGTGATGAAG
TGTAAGCAAG TGCCATTGGA CAAGAACGTT GTCTGTGTCA TTCCTTGTTG TATTTCCAAT
AGCAAGAGCA CATCAGATAC TTGGCAACTA GGCAGTCTTC CAGCTTCTGC CACTGCGCCC
GACTTCCTTG TTGCATGGAT TTATAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016884129.1
CDS431..1180
Translation

Target ORF information:

RefSeq Version XM_017028640.1
Organism Homo sapiens(Human)
Definition Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017028640.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGGAAC CCTTTGAAAA ACAGCAGATT GGCTCAAGTG CGATGCCATA TAAGCGGAAT 
CCCATGCGTT CAGAACGTTG CTGCAGTCTT GCCCGCCACC TGATGACCCT TGTCATGGAC
CCGCTACAGA CAGCATCTGT CCAGTGGTTT GAACGCACAC TGGATGATAG TGCCAACCGA
CGGATCTGTT TGGCCGAGGC ATTTCTTACC GCAGATACTA TATTGAATAC GCTGCAGAAC
ATTTCTGAAG GATTGGTCGT GTACCCCAAA GTAATTGAAC GGCGCATTCG GCAAGAGCTG
CCTTTCATGG CCACAGAGAA CATCATCATG GCCATGGTCA AAGCTGGAGG TAGCCGCCAG
GATTGCCATG AGAAAATCAG AGTGCTTTCT CAGCAGGCAG CTTCTGTGGT TAAGCAGGAA
GGGGGTGACA ATGACCTCAT AGAGCGTATC CAGGTTGATG CCTACTTCAG TCCCATTCAC
TCCCAGTTGG ATCATTTACT GGATCCTTCT TCTTTCACTG GTCGTGCCTC CCAGCAGGTG
CAGAGATTCT TAGAAGAGGA GGTGTATCCC CTGTTAAAAC CATATGAAAG CGTGATGAAG
TGTAAGCAAG TGCCATTGGA CAAGAACGTT GTCTGTGTCA TTCCTTGTTG TATTTCCAAT
AGCAAGAGCA CATCAGATAC TTGGCAACTA GGCAGTCTTC CAGCTTCTGC CACTGCGCCC
GACTTCCTTG TTGCATGGAT TTATAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu93395
Clone ID Related Accession (Same CDS sequence) XM_017028636.1
Accession Version XM_017028636.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1536bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product adenylosuccinate lyase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTCTT
GCCAGAGTGA TCTCTCGGCT TGCCGACTTT GCTAAGGAAC GAGCCAGTCT ACCCACATTA
GGTTTCACAC ATTTCCAGCC TGCACAGCTG ACCACAGTTG GGAAACGTTG CTGTCTTTGG
ATTCAGGATC TTTGCATGGA TCTCCAGAAC TTGAAGCGTG TCCGAGATGA CCTGCGCTTC
CGGGGAGTAA AGGGTACCAC TGGCACTCAG GCCAGTTTCC TGCAGCTCTT TGAGGGAGAT
GACCATAAGG TAGAGCAGCT TGACAAGATG GTGACAGAAA AGGCAGGATT TAAGAGAGCT
TTCATCATCA CAGGGCAGAC ATATACACGA AAAGTGGATA TTGAAGTACT GTCTGTGCTG
GCTAGCTTGG GGGCATCAGT GCACAAGATT TGCACCGACA TACGCCTCCT GGCAAACCTC
AAGGAGATGG AGGAACCCTT TGAAAAACAG CAGATTGGCT CAAGTGCGAT GCCATATAAG
CGGAATCCCA TGCGTTCAGA ACGTTGCTGC AGTCTTGCCC GCCACCTGAT GACCCTTGTC
ATGGACCCGC TACAGACAGC ATCTGTCCAG TGGTTTGAAC GCACACTGGA TGATAGTGCC
AACCGACGGA TCTGTTTGGC CGAGGCATTT CTTACCGCAG ATACTATATT GAATACGCTG
CAGAACATTT CTGAAGGATT GGTCGTGTAC CCCAAAGTAA TTGAACGGCG CATTCGGCAA
GAGCTGCCTT TCATGGCCAC AGAGAACATC ATCATGGCCA TGGTCAAAGC TGGAGGTAGC
CGCCAGGATT GCCATGAGAA AATCAGAGTG CTTTCTCAGC AGGCAGCTTC TGTGGTTAAG
CAGGAAGGGG GTGACAATGA CCTCATAGAG CGTATCCAGG TTGATGCCTA CTTCAGTCCC
ATTCACTCCC AGTTGGATCA TTTACTGGAT CCTTCTTCTT TCACTGGTCG TGCCTCCCAG
CAGGTGCAGA GATTCTTAGA AGAGGAGGTG TATCCCCTGT TAAAACCATA TGAAAGCGTG
ATGAAGTGTA AGCAAGTGCC ATTGGACAAG AACGTTGTCT GTGTCATTCC TTGTTGTATT
TCCAATAGCA AGAGCACATC AGATACTTGG CAACTAGGCA GTCTTCCAGC TTCTGCCACT
GCGCCCGACT TCCTTGTTGC ATGGATTTAT AGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016884125.1
CDS42..1577
Translation

Target ORF information:

RefSeq Version XM_017028636.1
Organism Homo sapiens(Human)
Definition Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017028636.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTCTT
GCCAGAGTGA TCTCTCGGCT TGCCGACTTT GCTAAGGAAC GAGCCAGTCT ACCCACATTA
GGTTTCACAC ATTTCCAGCC TGCACAGCTG ACCACAGTTG GGAAACGTTG CTGTCTTTGG
ATTCAGGATC TTTGCATGGA TCTCCAGAAC TTGAAGCGTG TCCGAGATGA CCTGCGCTTC
CGGGGAGTAA AGGGTACCAC TGGCACTCAG GCCAGTTTCC TGCAGCTCTT TGAGGGAGAT
GACCATAAGG TAGAGCAGCT TGACAAGATG GTGACAGAAA AGGCAGGATT TAAGAGAGCT
TTCATCATCA CAGGGCAGAC ATATACACGA AAAGTGGATA TTGAAGTACT GTCTGTGCTG
GCTAGCTTGG GGGCATCAGT GCACAAGATT TGCACCGACA TACGCCTCCT GGCAAACCTC
AAGGAGATGG AGGAACCCTT TGAAAAACAG CAGATTGGCT CAAGTGCGAT GCCATATAAG
CGGAATCCCA TGCGTTCAGA ACGTTGCTGC AGTCTTGCCC GCCACCTGAT GACCCTTGTC
ATGGACCCGC TACAGACAGC ATCTGTCCAG TGGTTTGAAC GCACACTGGA TGATAGTGCC
AACCGACGGA TCTGTTTGGC CGAGGCATTT CTTACCGCAG ATACTATATT GAATACGCTG
CAGAACATTT CTGAAGGATT GGTCGTGTAC CCCAAAGTAA TTGAACGGCG CATTCGGCAA
GAGCTGCCTT TCATGGCCAC AGAGAACATC ATCATGGCCA TGGTCAAAGC TGGAGGTAGC
CGCCAGGATT GCCATGAGAA AATCAGAGTG CTTTCTCAGC AGGCAGCTTC TGTGGTTAAG
CAGGAAGGGG GTGACAATGA CCTCATAGAG CGTATCCAGG TTGATGCCTA CTTCAGTCCC
ATTCACTCCC AGTTGGATCA TTTACTGGAT CCTTCTTCTT TCACTGGTCG TGCCTCCCAG
CAGGTGCAGA GATTCTTAGA AGAGGAGGTG TATCCCCTGT TAAAACCATA TGAAAGCGTG
ATGAAGTGTA AGCAAGTGCC ATTGGACAAG AACGTTGTCT GTGTCATTCC TTGTTGTATT
TCCAATAGCA AGAGCACATC AGATACTTGG CAACTAGGCA GTCTTCCAGC TTCTGCCACT
GCGCCCGACT TCCTTGTTGC ATGGATTTAT AGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism.
Nature communications53650(2014 Apr)
Corominas R,Yang X,Lin GN,Kang S,Shen Y,Ghamsari L,Broly M,Rodriguez M,Tam S,Trigg SA,Fan C,Yi S,Tasan M,Lemmens I,Kuang X,Zhao N,Malhotra D,Michaelson JJ,Vacic V,Calderwood MA,Roth FP,Tavernier J,Horvath S,Salehi-Ashtiani K,Korkin D,Sebat J,Hill DE,Hao T,Vidal M,Iakoucheva LM


book

Inherent properties of adenylosuccinate lyase could explain S-Ado/SAICAr ratio due to homozygous R426H and R303C mutations.
Biochimica et biophysica acta1834(8)1545-53(2013 Aug)
Ray SP,Duval N,Wilkinson TG 2nd,Shaheen SE,Ghosh K,Patterson D


book

Structural and biochemical characterization of human adenylosuccinate lyase (ADSL) and the R303C ADSL deficiency-associated mutation.
Biochemistry51(33)6701-13(2012 Aug)
Ray SP,Deaton MK,Capodagli GC,Calkins LA,Sawle L,Ghosh K,Patterson D,Pegan SD


book

Mutations of ATIC and ADSL affect purinosome assembly in cultured skin fibroblasts from patients with AICA-ribosiduria and ADSL deficiency.
Human molecular genetics21(7)1534-43(2012 Apr)
Baresova V,Skopova V,Sikora J,Patterson D,Sovova J,Zikanova M,Kmoch S


book

Adenylosuccinate lyase deficiency in the United Kingdom pediatric population: first three cases.
Pediatric neurology43(5)351-4(2010 Nov)
Lundy CT,Jungbluth H,Pohl KR,Siddiqui A,Marinaki AM,Mundy H,Champion MP


 
*
*
*
*