×

ERCC1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol ERCC1
Entrez Gene ID 2067
Full Name excision repair cross-complementation group 1
Synonyms COFS4, RAD10, UV20
General protein information
Preferred Names
DNA excision repair protein ERCC-1
Names
DNA excision repair protein ERCC-1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19q13.32

Summary The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009]. lac of sum
Disorder MIM:

126380

Disorder Html: Cerebrooculofacioskeletal syndrome 4, 610758 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_005258634 XP_005258691 DNA excision repair protein ERCC-1 isoform X1
XM_005258635 XP_005258692 DNA excision repair protein ERCC-1 isoform X1
XM_011526610 XP_011524912 DNA excision repair protein ERCC-1 isoform X1
XM_005258636 XP_005258693 DNA excision repair protein ERCC-1 isoform X1
XM_005258637 XP_005258694 DNA excision repair protein ERCC-1 isoform X2
NM_001983 NP_001974 DNA excision repair protein ERCC-1 isoform 2
NM_001166049 NP_001159521 DNA excision repair protein ERCC-1 isoform 3
NM_202001 NP_973730 DNA excision repair protein ERCC-1 isoform 1



Homo sapiens (human) ERCC1 NP_001974.1
Pan troglodytes (chimpanzee) ERCC1 NP_001181860.1
Canis lupus familiaris (dog) ERCC1 XP_005616504.1
Bos taurus (cattle) ERCC1 NP_001069853.1
Mus musculus (house mouse) Ercc1 NP_031974.2
Rattus norvegicus (Norway rat) Ercc1 NP_001099698.1
Danio rerio (zebrafish) ercc1 NP_001096608.1
Drosophila melanogaster (fruit fly) Ercc1 NP_477468.1
Arabidopsis thaliana (thale cress) ERCC1 NP_187172.1
Xenopus (Silurana) tropicalis (western clawed frog) ercc1 NP_001107393.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ERCC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ERCC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Free ORF Clone
for Every Scientist
More Detail >>

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu24783
XM_005258634 PREDICTED: Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu24783
XM_005258635 PREDICTED: Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu24783
XM_011526610 PREDICTED: Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu24783
XM_005258636 PREDICTED: Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu52101 XM_005258637 PREDICTED: Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu22115
NM_001983 Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu22964
NM_001166049 Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu24783
NM_202001 Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu24783
Clone ID Related Accession (Same CDS sequence) NM_202001 , XM_005258634 , XM_005258635 , XM_005258636 , XM_011526610
Accession Version XM_005258634.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 972bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA excision repair protein ERCC-1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011109.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGACCCTG GGAAGGACAA AGAGGGGGTG CCCCAGCCCT CAGGGCCGCC AGCAAGGAAG 
AAATTTGTGA TACCCCTCGA CGAGGATGAG GTCCCTCCTG GAGTGGCCAA GCCCTTATTC
CGATCTACAC AGAGCCTTCC CACTGTGGAC ACCTCGGCCC AGGCGGCCCC TCAGACCTAC
GCCGAATATG CCATCTCACA GCCTCTGGAA GGGGCTGGGG CCACGTGCCC CACAGGGTCA
GAGCCCCTGG CAGGAGAGAC GCCCAACCAG GCCCTGAAAC CCGGGGCAAA ATCCAACAGC
ATCATTGTGA GCCCTCGGCA GAGGGGCAAT CCCGTACTGA AGTTCGTGCG CAATGTGCCC
TGGGAATTTG GCGACGTAAT TCCCGACTAT GTGCTGGGCC AGAGCACCTG TGCCCTGTTC
CTCAGCCTCC GCTACCACAA CCTGCACCCA GACTACATCC ATGGGCGGCT GCAGAGCCTG
GGGAAGAACT TCGCCTTGCG GGTCCTGCTT GTCCAGGTGG ATGTGAAAGA TCCCCAGCAG
GCCCTCAAGG AGCTGGCTAA GATGTGTATC CTGGCCGACT GCACATTGAT CCTCGCCTGG
AGCCCCGAGG AAGCTGGGCG GTACCTGGAG ACCTACAAGG CCTATGAGCA GAAACCAGCG
GACCTCCTGA TGGAGAAGCT AGAGCAGGAC TTCGTCTCCC GGGTGACTGA ATGTCTGACC
ACCGTGAAGT CAGTCAACAA AACGGACAGT CAGACCCTCC TGACCACATT TGGATCTCTG
GAACAGCTCA TCGCCGCATC AAGAGAAGAT CTGGCCTTAT GCCCAGGCCT GGGCCCTCAG
AAAGTAAGAG CTCTGGGAAA GAACCCAAGG AGTTGGGGGA AGGAGAGAGC CCCAAATAAA
CACAACCTGA GACCCCAAAG TTTTAAGGTG AAAAAAGAAC CAAAGACCAG ACACAGTGGC
TTCCGCCTGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005258691.1
CDS78..1049
Misc Feature(1)372..707(+)
Misc Feature(2)792..932(+)
Translation

Target ORF information:

RefSeq Version XM_005258634
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005258634

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGACCCTG GGAAGGACAA AGAGGGGGTG CCCCAGCCCT CAGGGCCGCC AGCAAGGAAG 
AAATTTGTGA TACCCCTCGA CGAGGATGAG GTCCCTCCTG GAGTGGCCAA GCCCTTATTC
CGATCTACAC AGAGCCTTCC CACTGTGGAC ACCTCGGCCC AGGCGGCCCC TCAGACCTAC
GCCGAATATG CCATCTCACA GCCTCTGGAA GGGGCTGGGG CCACGTGCCC CACAGGGTCA
GAGCCCCTGG CAGGAGAGAC GCCCAACCAG GCCCTGAAAC CCGGGGCAAA ATCCAACAGC
ATCATTGTGA GCCCTCGGCA GAGGGGCAAT CCCGTACTGA AGTTCGTGCG CAATGTGCCC
TGGGAATTTG GCGACGTAAT TCCCGACTAT GTGCTGGGCC AGAGCACCTG TGCCCTGTTC
CTCAGCCTCC GCTACCACAA CCTGCACCCA GACTACATCC ATGGGCGGCT GCAGAGCCTG
GGGAAGAACT TCGCCTTGCG GGTCCTGCTT GTCCAGGTGG ATGTGAAAGA TCCCCAGCAG
GCCCTCAAGG AGCTGGCTAA GATGTGTATC CTGGCCGACT GCACATTGAT CCTCGCCTGG
AGCCCCGAGG AAGCTGGGCG GTACCTGGAG ACCTACAAGG CCTATGAGCA GAAACCAGCG
GACCTCCTGA TGGAGAAGCT AGAGCAGGAC TTCGTCTCCC GGGTGACTGA ATGTCTGACC
ACCGTGAAGT CAGTCAACAA AACGGACAGT CAGACCCTCC TGACCACATT TGGATCTCTG
GAACAGCTCA TCGCCGCATC AAGAGAAGAT CTGGCCTTAT GCCCAGGCCT GGGCCCTCAG
AAAGTAAGAG CTCTGGGAAA GAACCCAAGG AGTTGGGGGA AGGAGAGAGC CCCAAATAAA
CACAACCTGA GACCCCAAAG TTTTAAGGTG AAAAAAGAAC CAAAGACCAG ACACAGTGGC
TTCCGCCTGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24783
Clone ID Related Accession (Same CDS sequence) NM_202001 , XM_005258634 , XM_005258635 , XM_005258636 , XM_011526610
Accession Version XM_005258635.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 972bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA excision repair protein ERCC-1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011109.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530415961. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGACCCTG GGAAGGACAA AGAGGGGGTG CCCCAGCCCT CAGGGCCGCC AGCAAGGAAG 
AAATTTGTGA TACCCCTCGA CGAGGATGAG GTCCCTCCTG GAGTGGCCAA GCCCTTATTC
CGATCTACAC AGAGCCTTCC CACTGTGGAC ACCTCGGCCC AGGCGGCCCC TCAGACCTAC
GCCGAATATG CCATCTCACA GCCTCTGGAA GGGGCTGGGG CCACGTGCCC CACAGGGTCA
GAGCCCCTGG CAGGAGAGAC GCCCAACCAG GCCCTGAAAC CCGGGGCAAA ATCCAACAGC
ATCATTGTGA GCCCTCGGCA GAGGGGCAAT CCCGTACTGA AGTTCGTGCG CAATGTGCCC
TGGGAATTTG GCGACGTAAT TCCCGACTAT GTGCTGGGCC AGAGCACCTG TGCCCTGTTC
CTCAGCCTCC GCTACCACAA CCTGCACCCA GACTACATCC ATGGGCGGCT GCAGAGCCTG
GGGAAGAACT TCGCCTTGCG GGTCCTGCTT GTCCAGGTGG ATGTGAAAGA TCCCCAGCAG
GCCCTCAAGG AGCTGGCTAA GATGTGTATC CTGGCCGACT GCACATTGAT CCTCGCCTGG
AGCCCCGAGG AAGCTGGGCG GTACCTGGAG ACCTACAAGG CCTATGAGCA GAAACCAGCG
GACCTCCTGA TGGAGAAGCT AGAGCAGGAC TTCGTCTCCC GGGTGACTGA ATGTCTGACC
ACCGTGAAGT CAGTCAACAA AACGGACAGT CAGACCCTCC TGACCACATT TGGATCTCTG
GAACAGCTCA TCGCCGCATC AAGAGAAGAT CTGGCCTTAT GCCCAGGCCT GGGCCCTCAG
AAAGTAAGAG CTCTGGGAAA GAACCCAAGG AGTTGGGGGA AGGAGAGAGC CCCAAATAAA
CACAACCTGA GACCCCAAAG TTTTAAGGTG AAAAAAGAAC CAAAGACCAG ACACAGTGGC
TTCCGCCTGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005258692.1
CDS548..1519
Misc Feature(1)842..1177(+)
Misc Feature(2)1262..1402(+)
Translation

Target ORF information:

RefSeq Version XM_005258635
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005258635

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGACCCTG GGAAGGACAA AGAGGGGGTG CCCCAGCCCT CAGGGCCGCC AGCAAGGAAG 
AAATTTGTGA TACCCCTCGA CGAGGATGAG GTCCCTCCTG GAGTGGCCAA GCCCTTATTC
CGATCTACAC AGAGCCTTCC CACTGTGGAC ACCTCGGCCC AGGCGGCCCC TCAGACCTAC
GCCGAATATG CCATCTCACA GCCTCTGGAA GGGGCTGGGG CCACGTGCCC CACAGGGTCA
GAGCCCCTGG CAGGAGAGAC GCCCAACCAG GCCCTGAAAC CCGGGGCAAA ATCCAACAGC
ATCATTGTGA GCCCTCGGCA GAGGGGCAAT CCCGTACTGA AGTTCGTGCG CAATGTGCCC
TGGGAATTTG GCGACGTAAT TCCCGACTAT GTGCTGGGCC AGAGCACCTG TGCCCTGTTC
CTCAGCCTCC GCTACCACAA CCTGCACCCA GACTACATCC ATGGGCGGCT GCAGAGCCTG
GGGAAGAACT TCGCCTTGCG GGTCCTGCTT GTCCAGGTGG ATGTGAAAGA TCCCCAGCAG
GCCCTCAAGG AGCTGGCTAA GATGTGTATC CTGGCCGACT GCACATTGAT CCTCGCCTGG
AGCCCCGAGG AAGCTGGGCG GTACCTGGAG ACCTACAAGG CCTATGAGCA GAAACCAGCG
GACCTCCTGA TGGAGAAGCT AGAGCAGGAC TTCGTCTCCC GGGTGACTGA ATGTCTGACC
ACCGTGAAGT CAGTCAACAA AACGGACAGT CAGACCCTCC TGACCACATT TGGATCTCTG
GAACAGCTCA TCGCCGCATC AAGAGAAGAT CTGGCCTTAT GCCCAGGCCT GGGCCCTCAG
AAAGTAAGAG CTCTGGGAAA GAACCCAAGG AGTTGGGGGA AGGAGAGAGC CCCAAATAAA
CACAACCTGA GACCCCAAAG TTTTAAGGTG AAAAAAGAAC CAAAGACCAG ACACAGTGGC
TTCCGCCTGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24783
Clone ID Related Accession (Same CDS sequence) NM_202001 , XM_005258634 , XM_005258635 , XM_005258636 , XM_011526610
Accession Version XM_011526610.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 972bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA excision repair protein ERCC-1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011109.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGACCCTG GGAAGGACAA AGAGGGGGTG CCCCAGCCCT CAGGGCCGCC AGCAAGGAAG 
AAATTTGTGA TACCCCTCGA CGAGGATGAG GTCCCTCCTG GAGTGGCCAA GCCCTTATTC
CGATCTACAC AGAGCCTTCC CACTGTGGAC ACCTCGGCCC AGGCGGCCCC TCAGACCTAC
GCCGAATATG CCATCTCACA GCCTCTGGAA GGGGCTGGGG CCACGTGCCC CACAGGGTCA
GAGCCCCTGG CAGGAGAGAC GCCCAACCAG GCCCTGAAAC CCGGGGCAAA ATCCAACAGC
ATCATTGTGA GCCCTCGGCA GAGGGGCAAT CCCGTACTGA AGTTCGTGCG CAATGTGCCC
TGGGAATTTG GCGACGTAAT TCCCGACTAT GTGCTGGGCC AGAGCACCTG TGCCCTGTTC
CTCAGCCTCC GCTACCACAA CCTGCACCCA GACTACATCC ATGGGCGGCT GCAGAGCCTG
GGGAAGAACT TCGCCTTGCG GGTCCTGCTT GTCCAGGTGG ATGTGAAAGA TCCCCAGCAG
GCCCTCAAGG AGCTGGCTAA GATGTGTATC CTGGCCGACT GCACATTGAT CCTCGCCTGG
AGCCCCGAGG AAGCTGGGCG GTACCTGGAG ACCTACAAGG CCTATGAGCA GAAACCAGCG
GACCTCCTGA TGGAGAAGCT AGAGCAGGAC TTCGTCTCCC GGGTGACTGA ATGTCTGACC
ACCGTGAAGT CAGTCAACAA AACGGACAGT CAGACCCTCC TGACCACATT TGGATCTCTG
GAACAGCTCA TCGCCGCATC AAGAGAAGAT CTGGCCTTAT GCCCAGGCCT GGGCCCTCAG
AAAGTAAGAG CTCTGGGAAA GAACCCAAGG AGTTGGGGGA AGGAGAGAGC CCCAAATAAA
CACAACCTGA GACCCCAAAG TTTTAAGGTG AAAAAAGAAC CAAAGACCAG ACACAGTGGC
TTCCGCCTGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011524912.1
CDS80..1051
Misc Feature(1)374..709(+)
Misc Feature(2)794..934(+)
Translation

Target ORF information:

RefSeq Version XM_011526610
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011526610

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGACCCTG GGAAGGACAA AGAGGGGGTG CCCCAGCCCT CAGGGCCGCC AGCAAGGAAG 
AAATTTGTGA TACCCCTCGA CGAGGATGAG GTCCCTCCTG GAGTGGCCAA GCCCTTATTC
CGATCTACAC AGAGCCTTCC CACTGTGGAC ACCTCGGCCC AGGCGGCCCC TCAGACCTAC
GCCGAATATG CCATCTCACA GCCTCTGGAA GGGGCTGGGG CCACGTGCCC CACAGGGTCA
GAGCCCCTGG CAGGAGAGAC GCCCAACCAG GCCCTGAAAC CCGGGGCAAA ATCCAACAGC
ATCATTGTGA GCCCTCGGCA GAGGGGCAAT CCCGTACTGA AGTTCGTGCG CAATGTGCCC
TGGGAATTTG GCGACGTAAT TCCCGACTAT GTGCTGGGCC AGAGCACCTG TGCCCTGTTC
CTCAGCCTCC GCTACCACAA CCTGCACCCA GACTACATCC ATGGGCGGCT GCAGAGCCTG
GGGAAGAACT TCGCCTTGCG GGTCCTGCTT GTCCAGGTGG ATGTGAAAGA TCCCCAGCAG
GCCCTCAAGG AGCTGGCTAA GATGTGTATC CTGGCCGACT GCACATTGAT CCTCGCCTGG
AGCCCCGAGG AAGCTGGGCG GTACCTGGAG ACCTACAAGG CCTATGAGCA GAAACCAGCG
GACCTCCTGA TGGAGAAGCT AGAGCAGGAC TTCGTCTCCC GGGTGACTGA ATGTCTGACC
ACCGTGAAGT CAGTCAACAA AACGGACAGT CAGACCCTCC TGACCACATT TGGATCTCTG
GAACAGCTCA TCGCCGCATC AAGAGAAGAT CTGGCCTTAT GCCCAGGCCT GGGCCCTCAG
AAAGTAAGAG CTCTGGGAAA GAACCCAAGG AGTTGGGGGA AGGAGAGAGC CCCAAATAAA
CACAACCTGA GACCCCAAAG TTTTAAGGTG AAAAAAGAAC CAAAGACCAG ACACAGTGGC
TTCCGCCTGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24783
Clone ID Related Accession (Same CDS sequence) NM_202001 , XM_005258634 , XM_005258635 , XM_005258636 , XM_011526610
Accession Version XM_005258636.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 972bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA excision repair protein ERCC-1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011109.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578834109. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGACCCTG GGAAGGACAA AGAGGGGGTG CCCCAGCCCT CAGGGCCGCC AGCAAGGAAG 
AAATTTGTGA TACCCCTCGA CGAGGATGAG GTCCCTCCTG GAGTGGCCAA GCCCTTATTC
CGATCTACAC AGAGCCTTCC CACTGTGGAC ACCTCGGCCC AGGCGGCCCC TCAGACCTAC
GCCGAATATG CCATCTCACA GCCTCTGGAA GGGGCTGGGG CCACGTGCCC CACAGGGTCA
GAGCCCCTGG CAGGAGAGAC GCCCAACCAG GCCCTGAAAC CCGGGGCAAA ATCCAACAGC
ATCATTGTGA GCCCTCGGCA GAGGGGCAAT CCCGTACTGA AGTTCGTGCG CAATGTGCCC
TGGGAATTTG GCGACGTAAT TCCCGACTAT GTGCTGGGCC AGAGCACCTG TGCCCTGTTC
CTCAGCCTCC GCTACCACAA CCTGCACCCA GACTACATCC ATGGGCGGCT GCAGAGCCTG
GGGAAGAACT TCGCCTTGCG GGTCCTGCTT GTCCAGGTGG ATGTGAAAGA TCCCCAGCAG
GCCCTCAAGG AGCTGGCTAA GATGTGTATC CTGGCCGACT GCACATTGAT CCTCGCCTGG
AGCCCCGAGG AAGCTGGGCG GTACCTGGAG ACCTACAAGG CCTATGAGCA GAAACCAGCG
GACCTCCTGA TGGAGAAGCT AGAGCAGGAC TTCGTCTCCC GGGTGACTGA ATGTCTGACC
ACCGTGAAGT CAGTCAACAA AACGGACAGT CAGACCCTCC TGACCACATT TGGATCTCTG
GAACAGCTCA TCGCCGCATC AAGAGAAGAT CTGGCCTTAT GCCCAGGCCT GGGCCCTCAG
AAAGTAAGAG CTCTGGGAAA GAACCCAAGG AGTTGGGGGA AGGAGAGAGC CCCAAATAAA
CACAACCTGA GACCCCAAAG TTTTAAGGTG AAAAAAGAAC CAAAGACCAG ACACAGTGGC
TTCCGCCTGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005258693.1
CDS93..1064
Misc Feature(1)387..722(+)
Misc Feature(2)807..947(+)
Translation

Target ORF information:

RefSeq Version XM_005258636
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005258636

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGACCCTG GGAAGGACAA AGAGGGGGTG CCCCAGCCCT CAGGGCCGCC AGCAAGGAAG 
AAATTTGTGA TACCCCTCGA CGAGGATGAG GTCCCTCCTG GAGTGGCCAA GCCCTTATTC
CGATCTACAC AGAGCCTTCC CACTGTGGAC ACCTCGGCCC AGGCGGCCCC TCAGACCTAC
GCCGAATATG CCATCTCACA GCCTCTGGAA GGGGCTGGGG CCACGTGCCC CACAGGGTCA
GAGCCCCTGG CAGGAGAGAC GCCCAACCAG GCCCTGAAAC CCGGGGCAAA ATCCAACAGC
ATCATTGTGA GCCCTCGGCA GAGGGGCAAT CCCGTACTGA AGTTCGTGCG CAATGTGCCC
TGGGAATTTG GCGACGTAAT TCCCGACTAT GTGCTGGGCC AGAGCACCTG TGCCCTGTTC
CTCAGCCTCC GCTACCACAA CCTGCACCCA GACTACATCC ATGGGCGGCT GCAGAGCCTG
GGGAAGAACT TCGCCTTGCG GGTCCTGCTT GTCCAGGTGG ATGTGAAAGA TCCCCAGCAG
GCCCTCAAGG AGCTGGCTAA GATGTGTATC CTGGCCGACT GCACATTGAT CCTCGCCTGG
AGCCCCGAGG AAGCTGGGCG GTACCTGGAG ACCTACAAGG CCTATGAGCA GAAACCAGCG
GACCTCCTGA TGGAGAAGCT AGAGCAGGAC TTCGTCTCCC GGGTGACTGA ATGTCTGACC
ACCGTGAAGT CAGTCAACAA AACGGACAGT CAGACCCTCC TGACCACATT TGGATCTCTG
GAACAGCTCA TCGCCGCATC AAGAGAAGAT CTGGCCTTAT GCCCAGGCCT GGGCCCTCAG
AAAGTAAGAG CTCTGGGAAA GAACCCAAGG AGTTGGGGGA AGGAGAGAGC CCCAAATAAA
CACAACCTGA GACCCCAAAG TTTTAAGGTG AAAAAAGAAC CAAAGACCAG ACACAGTGGC
TTCCGCCTGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52101
Clone ID Related Accession (Same CDS sequence) XM_005258637
Accession Version XM_005258637.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 900bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA excision repair protein ERCC-1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011109.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACCCTG GGAAGGACAA AGAGGGGGTG CCCCAGCCCT CAGGGCCGCC AGCAAGGAAG 
AAATTTGTGA TACCCCTCGA CGAGGATGAG GTCCCTCCTG GAGTGGCCAA GCCCTTATTC
CGATCTACAC AGAGCCTTCC CACTGTGGAC ACCTCGGCCC AGGCGGCCCC TCAGACCTAC
GCCGAATATG CCATCTCACA GCCTCTGGAA GGGGCTGGGG CCACGTGCCC CACAGGGTCA
GAGCCCCTGG CAGGAGAGAC GCCCAACCAG GCCCTGAAAC CCGGGGCAAA ATCCAACAGC
ATCATTGTGA GCCCTCGGCA GAGGGGCAAT CCCGTACTGA AGTTCGTGCG CAATGTGCCC
TGGGAATTTG GCGACGTAAT TCCCGACTAT GTGCTGGGCC AGAGCACCTG TGCCCTGTTC
CTCAGCCTCC GCTACCACAA CCTGCACCCA GACTACATCC ATGGGCGGCT GCAGAGCCTG
GGGAAGAACT TCGCCTTGCG GGTCCTGCTT GTCCAGGTGG ATGTGAAAGA TCCCCAGCAG
GCCCTCAAGG AGCTGGCTAA GATGTGTATC CTGGCCGACT GCACATTGAT CCTCGCCTGG
AGCCCCGAGG AAGCTGGGCG GTACCTGGAG ACCTACAAGG CCTATGAGCA GAAACCAGCG
GACCTCCTGA TGGAGAAGCT AGAGCAGGAC TTCGTCTCCC GGTCTCTGGA ACAGCTCATC
GCCGCATCAA GAGAAGATCT GGCCTTATGC CCAGGCCTGG GCCCTCAGAA AGTAAGAGCT
CTGGGAAAGA ACCCAAGGAG TTGGGGGAAG GAGAGAGCCC CAAATAAACA CAACCTGAGA
CCCCAAAGTT TTAAGGTGAA AAAAGAACCA AAGACCAGAC ACAGTGGCTT CCGCCTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005258694.1
CDS78..977
Misc Feature(1)372..707(+)
Misc Feature(2)780..860(+)
Translation

Target ORF information:

RefSeq Version XM_005258637
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005258637

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACCCTG GGAAGGACAA AGAGGGGGTG CCCCAGCCCT CAGGGCCGCC AGCAAGGAAG 
AAATTTGTGA TACCCCTCGA CGAGGATGAG GTCCCTCCTG GAGTGGCCAA GCCCTTATTC
CGATCTACAC AGAGCCTTCC CACTGTGGAC ACCTCGGCCC AGGCGGCCCC TCAGACCTAC
GCCGAATATG CCATCTCACA GCCTCTGGAA GGGGCTGGGG CCACGTGCCC CACAGGGTCA
GAGCCCCTGG CAGGAGAGAC GCCCAACCAG GCCCTGAAAC CCGGGGCAAA ATCCAACAGC
ATCATTGTGA GCCCTCGGCA GAGGGGCAAT CCCGTACTGA AGTTCGTGCG CAATGTGCCC
TGGGAATTTG GCGACGTAAT TCCCGACTAT GTGCTGGGCC AGAGCACCTG TGCCCTGTTC
CTCAGCCTCC GCTACCACAA CCTGCACCCA GACTACATCC ATGGGCGGCT GCAGAGCCTG
GGGAAGAACT TCGCCTTGCG GGTCCTGCTT GTCCAGGTGG ATGTGAAAGA TCCCCAGCAG
GCCCTCAAGG AGCTGGCTAA GATGTGTATC CTGGCCGACT GCACATTGAT CCTCGCCTGG
AGCCCCGAGG AAGCTGGGCG GTACCTGGAG ACCTACAAGG CCTATGAGCA GAAACCAGCG
GACCTCCTGA TGGAGAAGCT AGAGCAGGAC TTCGTCTCCC GGTCTCTGGA ACAGCTCATC
GCCGCATCAA GAGAAGATCT GGCCTTATGC CCAGGCCTGG GCCCTCAGAA AGTAAGAGCT
CTGGGAAAGA ACCCAAGGAG TTGGGGGAAG GAGAGAGCCC CAAATAAACA CAACCTGAGA
CCCCAAAGTT TTAAGGTGAA AAAAGAACCA AAGACCAGAC ACAGTGGCTT CCGCCTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22115
Clone ID Related Accession (Same CDS sequence) NM_001983
Accession Version NM_001983.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 894bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product DNA excision repair protein ERCC-1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ671113.1, BC008930.2, AK314884.1, AC092309.3 and BQ019990.1. This sequence is a reference standard in the RefSeqGene project. On Oct 6, 2009 this sequence version replaced gi:42544170. Transcript Variant: This variant (2) represents the longest transcript and encodes isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF001925.1, M13194.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962337 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACCCTG GGAAGGACAA AGAGGGGGTG CCCCAGCCCT CAGGGCCGCC AGCAAGGAAG 
AAATTTGTGA TACCCCTCGA CGAGGATGAG GTCCCTCCTG GAGTGGCCAA GCCCTTATTC
CGATCTACAC AGAGCCTTCC CACTGTGGAC ACCTCGGCCC AGGCGGCCCC TCAGACCTAC
GCCGAATATG CCATCTCACA GCCTCTGGAA GGGGCTGGGG CCACGTGCCC CACAGGGTCA
GAGCCCCTGG CAGGAGAGAC GCCCAACCAG GCCCTGAAAC CCGGGGCAAA ATCCAACAGC
ATCATTGTGA GCCCTCGGCA GAGGGGCAAT CCCGTACTGA AGTTCGTGCG CAATGTGCCC
TGGGAATTTG GCGACGTAAT TCCCGACTAT GTGCTGGGCC AGAGCACCTG TGCCCTGTTC
CTCAGCCTCC GCTACCACAA CCTGCACCCA GACTACATCC ATGGGCGGCT GCAGAGCCTG
GGGAAGAACT TCGCCTTGCG GGTCCTGCTT GTCCAGGTGG ATGTGAAAGA TCCCCAGCAG
GCCCTCAAGG AGCTGGCTAA GATGTGTATC CTGGCCGACT GCACATTGAT CCTCGCCTGG
AGCCCCGAGG AAGCTGGGCG GTACCTGGAG ACCTACAAGG CCTATGAGCA GAAACCAGCG
GACCTCCTGA TGGAGAAGCT AGAGCAGGAC TTCGTCTCCC GGGTGACTGA ATGTCTGACC
ACCGTGAAGT CAGTCAACAA AACGGACAGT CAGACCCTCC TGACCACATT TGGATCTCTG
GAACAGCTCA TCGCCGCATC AAGAGAAGAT CTGGCCTTAT GCCCAGGCCT GGGCCCTCAG
AAAGCCCGGA GGCTGTTTGA TGTCCTGCAC GAGCCCTTCT TGAAAGTACC CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001974.1
CDS147..1040
Misc Feature(1)123..125(+)
Misc Feature(2)147..149(+)
Misc Feature(3)195..215(+)
Misc Feature(4)441..776(+)
Misc Feature(5)804..1037(+)
Misc Feature(6)861..1019(+)
Exon (1)1..139
Gene:ERCC1
Gene Synonym:
Exon (2)140..251
Gene:ERCC1
Gene Synonym:
Exon (3)252..467
Gene:ERCC1
Gene Synonym:
Exon (4)468..571
Gene:ERCC1
Gene Synonym:
Exon (5)572..671
Gene:ERCC1
Gene Synonym:
Exon (6)672..748
Gene:ERCC1
Gene Synonym:
Exon (7)749..848
Gene:ERCC1
Gene Synonym:
Exon (8)849..920
Gene:ERCC1
Gene Synonym:
Exon (9)921..989
Gene:ERCC1
Gene Synonym:
Exon (10)990..3382
Gene:ERCC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001983
Organism Homo sapiens (human)
Definition Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001983

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACCCTG GGAAGGACAA AGAGGGGGTG CCCCAGCCCT CAGGGCCGCC AGCAAGGAAG 
AAATTTGTGA TACCCCTCGA CGAGGATGAG GTCCCTCCTG GAGTGGCCAA GCCCTTATTC
CGATCTACAC AGAGCCTTCC CACTGTGGAC ACCTCGGCCC AGGCGGCCCC TCAGACCTAC
GCCGAATATG CCATCTCACA GCCTCTGGAA GGGGCTGGGG CCACGTGCCC CACAGGGTCA
GAGCCCCTGG CAGGAGAGAC GCCCAACCAG GCCCTGAAAC CCGGGGCAAA ATCCAACAGC
ATCATTGTGA GCCCTCGGCA GAGGGGCAAT CCCGTACTGA AGTTCGTGCG CAATGTGCCC
TGGGAATTTG GCGACGTAAT TCCCGACTAT GTGCTGGGCC AGAGCACCTG TGCCCTGTTC
CTCAGCCTCC GCTACCACAA CCTGCACCCA GACTACATCC ATGGGCGGCT GCAGAGCCTG
GGGAAGAACT TCGCCTTGCG GGTCCTGCTT GTCCAGGTGG ATGTGAAAGA TCCCCAGCAG
GCCCTCAAGG AGCTGGCTAA GATGTGTATC CTGGCCGACT GCACATTGAT CCTCGCCTGG
AGCCCCGAGG AAGCTGGGCG GTACCTGGAG ACCTACAAGG CCTATGAGCA GAAACCAGCG
GACCTCCTGA TGGAGAAGCT AGAGCAGGAC TTCGTCTCCC GGGTGACTGA ATGTCTGACC
ACCGTGAAGT CAGTCAACAA AACGGACAGT CAGACCCTCC TGACCACATT TGGATCTCTG
GAACAGCTCA TCGCCGCATC AAGAGAAGAT CTGGCCTTAT GCCCAGGCCT GGGCCCTCAG
AAAGCCCGGA GGCTGTTTGA TGTCCTGCAC GAGCCCTTCT TGAAAGTACC CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22964
Clone ID Related Accession (Same CDS sequence) NM_001166049
Accession Version NM_001166049.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 822bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product DNA excision repair protein ERCC-1 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ671113.1, AK314884.1, AB069681.1, AC092309.3 and BQ019990.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region, compared to variant 2. The resulting isoform (3) lacks an internal segment, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB069681.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGACCCTG GGAAGGACAA AGAGGGGGTG CCCCAGCCCT CAGGGCCGCC AGCAAGGAAG 
AAATTTGTGA TACCCCTCGA CGAGGATGAG GTCCCTCCTG GAGTGGCCAA GCCCTTATTC
CGATCTACAC AGAGCCTTCC CACTGTGGAC ACCTCGGCCC AGGCGGCCCC TCAGACCTAC
GCCGAATATG CCATCTCACA GCCTCTGGAA GGGGCTGGGG CCACGTGCCC CACAGGGTCA
GAGCCCCTGG CAGGAGAGAC GCCCAACCAG GCCCTGAAAC CCGGGGCAAA ATCCAACAGC
ATCATTGTGA GCCCTCGGCA GAGGGGCAAT CCCGTACTGA AGTTCGTGCG CAATGTGCCC
TGGGAATTTG GCGACGTAAT TCCCGACTAT GTGCTGGGCC AGAGCACCTG TGCCCTGTTC
CTCAGCCTCC GCTACCACAA CCTGCACCCA GACTACATCC ATGGGCGGCT GCAGAGCCTG
GGGAAGAACT TCGCCTTGCG GGTCCTGCTT GTCCAGGTGG ATGTGAAAGA TCCCCAGCAG
GCCCTCAAGG AGCTGGCTAA GATGTGTATC CTGGCCGACT GCACATTGAT CCTCGCCTGG
AGCCCCGAGG AAGCTGGGCG GTACCTGGAG ACCTACAAGG CCTATGAGCA GAAACCAGCG
GACCTCCTGA TGGAGAAGCT AGAGCAGGAC TTCGTCTCCC GGTCTCTGGA ACAGCTCATC
GCCGCATCAA GAGAAGATCT GGCCTTATGC CCAGGCCTGG GCCCTCAGAA AGCCCGGAGG
CTGTTTGATG TCCTGCACGA GCCCTTCTTG AAAGTACCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001159521.1
CDS147..968
Misc Feature(1)123..125(+)
Misc Feature(2)441..776(+)
Misc Feature(3)849..938(+)
Exon (1)1..139
Gene:ERCC1
Gene Synonym:
Exon (2)140..251
Gene:ERCC1
Gene Synonym:
Exon (3)252..467
Gene:ERCC1
Gene Synonym:
Exon (4)468..571
Gene:ERCC1
Gene Synonym:
Exon (5)572..671
Gene:ERCC1
Gene Synonym:
Exon (6)672..748
Gene:ERCC1
Gene Synonym:
Exon (7)749..848
Gene:ERCC1
Gene Synonym:
Exon (8)849..917
Gene:ERCC1
Gene Synonym:
Exon (9)918..3310
Gene:ERCC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001166049
Organism Homo sapiens (human)
Definition Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166049

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGACCCTG GGAAGGACAA AGAGGGGGTG CCCCAGCCCT CAGGGCCGCC AGCAAGGAAG 
AAATTTGTGA TACCCCTCGA CGAGGATGAG GTCCCTCCTG GAGTGGCCAA GCCCTTATTC
CGATCTACAC AGAGCCTTCC CACTGTGGAC ACCTCGGCCC AGGCGGCCCC TCAGACCTAC
GCCGAATATG CCATCTCACA GCCTCTGGAA GGGGCTGGGG CCACGTGCCC CACAGGGTCA
GAGCCCCTGG CAGGAGAGAC GCCCAACCAG GCCCTGAAAC CCGGGGCAAA ATCCAACAGC
ATCATTGTGA GCCCTCGGCA GAGGGGCAAT CCCGTACTGA AGTTCGTGCG CAATGTGCCC
TGGGAATTTG GCGACGTAAT TCCCGACTAT GTGCTGGGCC AGAGCACCTG TGCCCTGTTC
CTCAGCCTCC GCTACCACAA CCTGCACCCA GACTACATCC ATGGGCGGCT GCAGAGCCTG
GGGAAGAACT TCGCCTTGCG GGTCCTGCTT GTCCAGGTGG ATGTGAAAGA TCCCCAGCAG
GCCCTCAAGG AGCTGGCTAA GATGTGTATC CTGGCCGACT GCACATTGAT CCTCGCCTGG
AGCCCCGAGG AAGCTGGGCG GTACCTGGAG ACCTACAAGG CCTATGAGCA GAAACCAGCG
GACCTCCTGA TGGAGAAGCT AGAGCAGGAC TTCGTCTCCC GGTCTCTGGA ACAGCTCATC
GCCGCATCAA GAGAAGATCT GGCCTTATGC CCAGGCCTGG GCCCTCAGAA AGCCCGGAGG
CTGTTTGATG TCCTGCACGA GCCCTTCTTG AAAGTACCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24783
Clone ID Related Accession (Same CDS sequence) NM_202001 , XM_005258634 , XM_005258635 , XM_005258636 , XM_011526610
Accession Version NM_202001.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 972bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product DNA excision repair protein ERCC-1 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB468563.1, BC052813.1, AK314884.1 and BU627436.1. On Oct 6, 2009 this sequence version replaced gi:42544168. Transcript Variant: This variant (1) represents use of an alternate promoter and 5' UTR and uses a distinct splice pattern in the 3' coding region and 3' UTR, compared to variant 2. The resulting isoform (1) has a longer and distinct C-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052813.1, BM556010.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962337 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGACCCTG GGAAGGACAA AGAGGGGGTG CCCCAGCCCT CAGGGCCGCC AGCAAGGAAG 
AAATTTGTGA TACCCCTCGA CGAGGATGAG GTCCCTCCTG GAGTGGCCAA GCCCTTATTC
CGATCTACAC AGAGCCTTCC CACTGTGGAC ACCTCGGCCC AGGCGGCCCC TCAGACCTAC
GCCGAATATG CCATCTCACA GCCTCTGGAA GGGGCTGGGG CCACGTGCCC CACAGGGTCA
GAGCCCCTGG CAGGAGAGAC GCCCAACCAG GCCCTGAAAC CCGGGGCAAA ATCCAACAGC
ATCATTGTGA GCCCTCGGCA GAGGGGCAAT CCCGTACTGA AGTTCGTGCG CAATGTGCCC
TGGGAATTTG GCGACGTAAT TCCCGACTAT GTGCTGGGCC AGAGCACCTG TGCCCTGTTC
CTCAGCCTCC GCTACCACAA CCTGCACCCA GACTACATCC ATGGGCGGCT GCAGAGCCTG
GGGAAGAACT TCGCCTTGCG GGTCCTGCTT GTCCAGGTGG ATGTGAAAGA TCCCCAGCAG
GCCCTCAAGG AGCTGGCTAA GATGTGTATC CTGGCCGACT GCACATTGAT CCTCGCCTGG
AGCCCCGAGG AAGCTGGGCG GTACCTGGAG ACCTACAAGG CCTATGAGCA GAAACCAGCG
GACCTCCTGA TGGAGAAGCT AGAGCAGGAC TTCGTCTCCC GGGTGACTGA ATGTCTGACC
ACCGTGAAGT CAGTCAACAA AACGGACAGT CAGACCCTCC TGACCACATT TGGATCTCTG
GAACAGCTCA TCGCCGCATC AAGAGAAGAT CTGGCCTTAT GCCCAGGCCT GGGCCCTCAG
AAAGTAAGAG CTCTGGGAAA GAACCCAAGG AGTTGGGGGA AGGAGAGAGC CCCAAATAAA
CACAACCTGA GACCCCAAAG TTTTAAGGTG AAAAAAGAAC CAAAGACCAG ACACAGTGGC
TTCCGCCTGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_973730.1
CDS189..1160
Misc Feature(1)66..68(+)
Misc Feature(2)189..191(+)
Misc Feature(3)237..257(+)
Misc Feature(4)483..818(+)
Misc Feature(5)903..1043(+)
Exon (1)1..293
Gene:ERCC1
Gene Synonym:
Exon (2)294..509
Gene:ERCC1
Gene Synonym:
Exon (3)510..613
Gene:ERCC1
Gene Synonym:
Exon (4)614..713
Gene:ERCC1
Gene Synonym:
Exon (5)714..790
Gene:ERCC1
Gene Synonym:
Exon (6)791..890
Gene:ERCC1
Gene Synonym:
Exon (7)891..962
Gene:ERCC1
Gene Synonym:
Exon (8)963..1274
Gene:ERCC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_202001
Organism Homo sapiens (human)
Definition Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_202001

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGACCCTG GGAAGGACAA AGAGGGGGTG CCCCAGCCCT CAGGGCCGCC AGCAAGGAAG 
AAATTTGTGA TACCCCTCGA CGAGGATGAG GTCCCTCCTG GAGTGGCCAA GCCCTTATTC
CGATCTACAC AGAGCCTTCC CACTGTGGAC ACCTCGGCCC AGGCGGCCCC TCAGACCTAC
GCCGAATATG CCATCTCACA GCCTCTGGAA GGGGCTGGGG CCACGTGCCC CACAGGGTCA
GAGCCCCTGG CAGGAGAGAC GCCCAACCAG GCCCTGAAAC CCGGGGCAAA ATCCAACAGC
ATCATTGTGA GCCCTCGGCA GAGGGGCAAT CCCGTACTGA AGTTCGTGCG CAATGTGCCC
TGGGAATTTG GCGACGTAAT TCCCGACTAT GTGCTGGGCC AGAGCACCTG TGCCCTGTTC
CTCAGCCTCC GCTACCACAA CCTGCACCCA GACTACATCC ATGGGCGGCT GCAGAGCCTG
GGGAAGAACT TCGCCTTGCG GGTCCTGCTT GTCCAGGTGG ATGTGAAAGA TCCCCAGCAG
GCCCTCAAGG AGCTGGCTAA GATGTGTATC CTGGCCGACT GCACATTGAT CCTCGCCTGG
AGCCCCGAGG AAGCTGGGCG GTACCTGGAG ACCTACAAGG CCTATGAGCA GAAACCAGCG
GACCTCCTGA TGGAGAAGCT AGAGCAGGAC TTCGTCTCCC GGGTGACTGA ATGTCTGACC
ACCGTGAAGT CAGTCAACAA AACGGACAGT CAGACCCTCC TGACCACATT TGGATCTCTG
GAACAGCTCA TCGCCGCATC AAGAGAAGAT CTGGCCTTAT GCCCAGGCCT GGGCCCTCAG
AAAGTAAGAG CTCTGGGAAA GAACCCAAGG AGTTGGGGGA AGGAGAGAGC CCCAAATAAA
CACAACCTGA GACCCCAAAG TTTTAAGGTG AAAAAAGAAC CAAAGACCAG ACACAGTGGC
TTCCGCCTGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
*
*
*
*