ISCU cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Gene Symbol	ISCU
Entrez Gene ID	23479
Full Name	iron-sulfur cluster assembly enzyme
Synonyms	2310020H20Rik,HML,ISU2,NIFU,NIFUN,hnifU
General protein information	Preferred Names iron-sulfur cluster assembly enzyme Names iron-sulfur cluster assembly enzyme ISCU, mitochondrial IscU iron-sulfur cluster scaffold homolog nifU-like N-terminal domain-containing protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q23.3
Summary	This gene encodes a component of the iron-sulfur (Fe-S) cluster scaffold. Fe-S clusters are cofactors that play a role in the function of a diverse set of enzymes, including those that regulate metabolism, iron homeostasis, and oxidative stress response. Alternative splicing results in transcript variants encoding different protein isoforms that localize either to the cytosol or to the mitochondrion. Mutations in this gene have been found in patients with hereditary myopathy with lactic acidosis. A disease-associated mutation in an intron may activate a cryptic splice site, resulting in the production of a splice variant encoding a putatively non-functional protein. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Feb 2016].
Disorder MIM:	611911
Disorder Html:	Myopathy with lactic acidosis, hereditary, 255125 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011538101.2	XP_011536403.1	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform X2
NM_001301141.1	NP_001288070.1	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform 4 precursor
XM_011538100.2	XP_011536402.1	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform X1
NM_014301.4	NP_055116.1	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform 1
NM_001301140.1	NP_001288069.1	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform 3 precursor
NM_001320042.1	NP_001306971.1	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform 3 precursor
NM_213595.3	NP_998760.1	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform 2 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ISCU	XP_509343.2
Canis lupus familiaris (dog)	LOC477527	XP_534722.1
Mus musculus (house mouse)	Iscu	NP_079802.1
Drosophila melanogaster (fruit fly)	CG9836	NP_649840.1
Oryza sativa (rice)	Os01g0662600	NP_001043783.1
Oryza sativa (rice)	Os05g0568100	NP_001056355.1
Xenopus tropicalis (tropical clawed frog)	iscu	NP_989088.1
Bos taurus (cattle)	ISCU	NP_001069151.1
Danio rerio (zebrafish)	iscub	NP_001017800.2
Caenorhabditis elegans (roundworm)	Y45F10D.4	NP_502658.1
Saccharomyces cerevisiae (baker's yeast)	ISU1	NP_015190.1
Danio rerio (zebrafish)	si:ch211-191d15.2	NP_001003632.1
Saccharomyces cerevisiae (baker's yeast)	ISU2	NP_014869.3
Eremothecium gossypii	AGOS_ACR112C	NP_983514.1
Neurospora crassa	NCU05778	XP_960038.1
Homo sapiens (human)	ISCU	NP_998760.1
Rattus norvegicus (Norway rat)	Iscu	NP_001099406.1
Gallus gallus (chicken)	ISCU	XP_003642230.2
Arabidopsis thaliana (thale cress)	ISU1	NP_193953.1
Arabidopsis thaliana (thale cress)	ISU2	NP_186751.1
	ISCU	XP_001103180.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005813	XP_315834.2
Kluyveromyces lactis	KLLA0D07161g	XP_453380.1
Schizosaccharomyces pombe (fission yeast)	isu1	NP_592967.1
Arabidopsis thaliana (thale cress)	ISU3	NP_192317.1

Gene Ontology
Bibliography

Related articles in PubMed

Iron-sulfur cluster biogenesis in mammalian cells: New insights into the molecular mechanisms of cluster delivery.
Maio N, Rouault TA
Biochimica et biophysica acta1853(6)1493-512(2015 Jun)

Tissue specificity of a human mitochondrial disease: differentiation-enhanced mis-splicing of the Fe-S scaffold gene ISCU renders patient cells more sensitive to oxidative stress in ISCU myopathy.
Crooks DR, Jeong SY, Tong WH, Ghosh MC, Olivierre H, Haller RG, Rouault TA
The Journal of biological chemistry287(48)40119-30(2012 Nov)

Tissue-specific splicing of ISCU results in a skeletal muscle phenotype in myopathy with lactic acidosis, while complete loss of ISCU results in early embryonic death in mice.
Nordin A, Larsson E, Thornell LE, Holmberg M
Human genetics129(4)371-8(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The NFS1/ISD11 complex further interacts with scaffold protein ISCU and regulator protein frataxin, thereby forming a quaternary complex for Fe-S cluster formation.
Title: The N-Terminus of Iron-Sulfur Cluster Assembly Factor ISD11 Is Crucial for Subcellular Targeting and Interaction with l-Cysteine Desulfurase NFS1.

We have shown that ASO treatment diminished aberrant splicing and increased ISCU protein levels in both patient fibroblasts and patient myotubes in a concentration dependent fashion. Upon ASO treatment, levels of SDHB in patient myotubular cell lines increased to levels observed in control myotubular cell lines
Title: Use of antisense oligonucleotides to correct the splicing error in ISCU myopathy patient cell lines.

Molecular dynamics flexible fitting of protein structures docked into the EM map of the model revealed a [FXN(42-210)]24.[NFS1]24.[ISD11]24.[ISCU]24 complex, consistent with the measured 1:1:1:1 stoichiometry of its four components.
Title: Architecture of the Human Mitochondrial Iron-Sulfur Cluster Assembly Machinery.

ISCU expression was decreased in the majority of human liver cancer tissues, and its reduced expression was significantly associated with p53 mutation.
Title: Regulation of iron homeostasis by the p53-ISCU pathway.

The core Fe-S biosynthetic enzymatic complex generated [2Fe-2S] cluster intermediates that converted to stable [2Fe-2S] clusters bound to uncomplexed ISCU2.
Title: The Human Iron-Sulfur Assembly Complex Catalyzes the Synthesis of [2Fe-2S] Clusters on ISCU2 That Can Be Transferred to Acceptor Molecules.

The following ISCU gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ISCU cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22063	NM_213595.3 Latest version!	Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22179	NM_014301.4 Latest version!	Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu46446	NM_001301140.1 Latest version!	Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu46446	NM_001320042.1 Latest version!	Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu46445	NM_001301141.1 Latest version!	Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu46447	XM_011538100.2 Latest version!	Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu66215	XM_011538101.2 Latest version!	Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ISCU ( NM_213595.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_998760.1 Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant 2, mRNA.

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CloneID	OHu22063
Clone ID Related Accession (Same CDS sequence)	NM_213595.3
Accession Version	NM_213595.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK057251.1, BC011906.2 and BQ020993.1. On Jul 23, 2014 this sequence version replaced NM_213595.2. Transcript Variant: This variant (2) encodes the longest isoform (2). Isoform 2 is localized to the mitochondrion. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.584534.1, SRR1163658.132578.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CTGGGGCTTT CCGTCTGAGG CGGGCGGCAT CGGCTCTGCT GCTGCGGAGC 
CCCCGCCTGC CCGCCCGGGA GCTGTCGGCC CCGGCCCGAC TCTATCACAA GAAGGTTGTT 
GATCATTATG AAAATCCTAG AAACGTGGGG TCCCTTGACA AGACATCTAA AAATGTTGGA 
ACTGGACTGG TGGGGGCTCC AGCATGTGGT GACGTAATGA AATTACAGAT TCAAGTGGAT 
GAAAAGGGGA AGATTGTGGA TGCTAGGTTT AAAACATTTG GCTGTGGTTC CGCAATTGCC 
TCCAGCTCAT TAGCCACTGA ATGGGTGAAA GGAAAGACGG TGGAGGAAGC CTTGACTATC 
AAAAACACAG ATATCGCCAA GGAGCTCTGC CTTCCTCCCG TGAAACTGCA CTGCTCCATG 
CTGGCTGAAG ATGCAATCAA GGCCGCCCTG GCTGATTACA AATTGAAACA AGAACCCAAA 
AAAGGAGAGG CAGAGAAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998760.1
CDS	1161..1664
Translation	MAAAGAFRLRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQEPKKGEAEKK

Target ORF information:

RefSeq Version	NM_213595.3
Organism	Homo sapiens(human)
Definition	Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213595.3

ORF Insert Sequence:

ATGGCGGCGG CTGGGGCTTT CCGTCTGAGG CGGGCGGCAT CGGCTCTGCT GCTGCGGAGC 
CCCCGCCTGC CCGCCCGGGA GCTGTCGGCC CCGGCCCGAC TCTATCACAA GAAGGTTGTT 
GATCATTATG AAAATCCTAG AAACGTGGGG TCCCTTGACA AGACATCTAA AAATGTTGGA 
ACTGGACTGG TGGGGGCTCC AGCATGTGGT GACGTAATGA AATTACAGAT TCAAGTGGAT 
GAAAAGGGGA AGATTGTGGA TGCTAGGTTT AAAACATTTG GCTGTGGTTC CGCAATTGCC 
TCCAGCTCAT TAGCCACTGA ATGGGTGAAA GGAAAGACGG TGGAGGAAGC CTTGACTATC 
AAAAACACAG ATATCGCCAA GGAGCTCTGC CTTCCTCCCG TGAAACTGCA CTGCTCCATG 
CTGGCTGAAG ATGCAATCAA GGCCGCCCTG GCTGATTACA AATTGAAACA AGAACCCAAA 
AAAGGAGAGG CAGAGAAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISCU ( NM_014301.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055116.1 Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22179
Clone ID Related Accession (Same CDS sequence)	NM_014301.4
Accession Version	NM_014301.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK057251.1, AY009127.1 and BQ020993.1. This sequence is a reference standard in the RefSeqGene project. On Jul 23, 2014 this sequence version replaced NM_014301.3. Transcript Variant: This variant (1) contains an alternate exon in the 5' region and initiates translation at an alternate start codon compared to variant 2. The encoded isoform (1) has a distinct, shorter N-terminus, compared to isoform 2. Isoform 1 is localized to the cytosol. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY009127.1, SRR1163658.280581.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTTCTCA TTGACATGAG TGTAGACCTT TCTACTCAGG TTGTTGATCA TTATGAAAAT 
CCTAGAAACG TGGGGTCCCT TGACAAGACA TCTAAAAATG TTGGAACTGG ACTGGTGGGG 
GCTCCAGCAT GTGGTGACGT AATGAAATTA CAGATTCAAG TGGATGAAAA GGGGAAGATT 
GTGGATGCTA GGTTTAAAAC ATTTGGCTGT GGTTCCGCAA TTGCCTCCAG CTCATTAGCC 
ACTGAATGGG TGAAAGGAAA GACGGTGGAG GAAGCCTTGA CTATCAAAAA CACAGATATC 
GCCAAGGAGC TCTGCCTTCC TCCCGTGAAA CTGCACTGCT CCATGCTGGC TGAAGATGCA 
ATCAAGGCCG CCCTGGCTGA TTACAAATTG AAACAAGAAC CCAAAAAAGG AGAGGCAGAG 
AAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055116.1
CDS	1332..1760
Translation	MVLIDMSVDLSTQVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQEPKKGEAEKK

Target ORF information:

RefSeq Version	NM_014301.4
Organism	Homo sapiens(human)
Definition	Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014301.4

ORF Insert Sequence:

ATGGTTCTCA TTGACATGAG TGTAGACCTT TCTACTCAGG TTGTTGATCA TTATGAAAAT 
CCTAGAAACG TGGGGTCCCT TGACAAGACA TCTAAAAATG TTGGAACTGG ACTGGTGGGG 
GCTCCAGCAT GTGGTGACGT AATGAAATTA CAGATTCAAG TGGATGAAAA GGGGAAGATT 
GTGGATGCTA GGTTTAAAAC ATTTGGCTGT GGTTCCGCAA TTGCCTCCAG CTCATTAGCC 
ACTGAATGGG TGAAAGGAAA GACGGTGGAG GAAGCCTTGA CTATCAAAAA CACAGATATC 
GCCAAGGAGC TCTGCCTTCC TCCCGTGAAA CTGCACTGCT CCATGCTGGC TGAAGATGCA 
ATCAAGGCCG CCCTGGCTGA TTACAAATTG AAACAAGAAC CCAAAAAAGG AGAGGCAGAG 
AAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISCU ( NM_001301140.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001288069.1 Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu46446
Clone ID Related Accession (Same CDS sequence)	NM_001301140.1 , NM_001320042.1
Accession Version	NM_001301140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Homo sapiens(human)
Product	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK057251.1, BG702203.1 and BQ020993.1. Transcript Variant: This variant (3) contains an alternate exon in the 3' region, resulting in a different 3' coding region and 3' UTR, compared to variant 2. Variants 3 and 5 encode the same protein (isoform 3), which has a distinct, shorter C-terminus, compared to isoform 2. Isoform 3 may not be stable (PMID:23035118). This variant may be preferentially produced in individuals containing an alternate intronic C vs G (rs767000507), which activates a cryptic splice acceptor site. The alternate C allele is associated with myopathy (PMIDs 18296749, 18304497). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.373269.1, BG702203.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CTGGGGCTTT CCGTCTGAGG CGGGCGGCAT CGGCTCTGCT GCTGCGGAGC 
CCCCGCCTGC CCGCCCGGGA GCTGTCGGCC CCGGCCCGAC TCTATCACAA GAAGGTTGTT 
GATCATTATG AAAATCCTAG AAACGTGGGG TCCCTTGACA AGACATCTAA AAATGTTGGA 
ACTGGACTGG TGGGGGCTCC AGCATGTGGT GACGTAATGA AATTACAGAT TCAAGTGGAT 
GAAAAGGGGA AGATTGTGGA TGCTAGGTTT AAAACATTTG GCTGTGGTTC CGCAATTGCC 
TCCAGCTCAT TAGCCACTGA ATGGGTGAAA GGAAAGACGG TGGAGGAAGC CTTGACTATC 
AAAAACACAG ATATCGCCAA GGAGCTCTGC CTTCCTCCCG TGAAACTGCA CTGCTCCAAA 
TCTGTGCTGT TTCCAGCAGA GGAGAAAACT CAGCTTTCGC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288069.1
CDS	1161..1625
Translation	MAAAGAFRLRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSKSVLFPAEEKTQLSP

Target ORF information:

RefSeq Version	NM_001301140.1
Organism	Homo sapiens(human)
Definition	Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301140.1

ORF Insert Sequence:

ATGGCGGCGG CTGGGGCTTT CCGTCTGAGG CGGGCGGCAT CGGCTCTGCT GCTGCGGAGC 
CCCCGCCTGC CCGCCCGGGA GCTGTCGGCC CCGGCCCGAC TCTATCACAA GAAGGTTGTT 
GATCATTATG AAAATCCTAG AAACGTGGGG TCCCTTGACA AGACATCTAA AAATGTTGGA 
ACTGGACTGG TGGGGGCTCC AGCATGTGGT GACGTAATGA AATTACAGAT TCAAGTGGAT 
GAAAAGGGGA AGATTGTGGA TGCTAGGTTT AAAACATTTG GCTGTGGTTC CGCAATTGCC 
TCCAGCTCAT TAGCCACTGA ATGGGTGAAA GGAAAGACGG TGGAGGAAGC CTTGACTATC 
AAAAACACAG ATATCGCCAA GGAGCTCTGC CTTCCTCCCG TGAAACTGCA CTGCTCCAAA 
TCTGTGCTGT TTCCAGCAGA GGAGAAAACT CAGCTTTCGC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISCU ( NM_001320042.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001306971.1 Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu46446
Clone ID Related Accession (Same CDS sequence)	NM_001301140.1 , NM_001320042.1
Accession Version	NM_001320042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Homo sapiens(human)
Product	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK057251.1, AK297862.1, HY269565.1 and BC061903.1. On Feb 10, 2016 this sequence version replaced XM_005268761.3. Transcript Variant: This variant (5) uses an alternate splice site in the 3' region, resulting in a different 3' coding region and 3' UTR, compared to variant 2. Variants 3 and 5 encode the same protein (isoform 3), which has a distinct, shorter C-terminus, compared to isoform 2. Isoform 3 may not be stable (PMID:23035118). This variant may be preferentially produced in individuals containing an alternate intronic C vs G (rs767000507), which activates a cryptic splice acceptor site. The alternate C allele is associated with myopathy (PMIDs 18296749, 18304497). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.184875.1, SRR1660807.19899.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CTGGGGCTTT CCGTCTGAGG CGGGCGGCAT CGGCTCTGCT GCTGCGGAGC 
CCCCGCCTGC CCGCCCGGGA GCTGTCGGCC CCGGCCCGAC TCTATCACAA GAAGGTTGTT 
GATCATTATG AAAATCCTAG AAACGTGGGG TCCCTTGACA AGACATCTAA AAATGTTGGA 
ACTGGACTGG TGGGGGCTCC AGCATGTGGT GACGTAATGA AATTACAGAT TCAAGTGGAT 
GAAAAGGGGA AGATTGTGGA TGCTAGGTTT AAAACATTTG GCTGTGGTTC CGCAATTGCC 
TCCAGCTCAT TAGCCACTGA ATGGGTGAAA GGAAAGACGG TGGAGGAAGC CTTGACTATC 
AAAAACACAG ATATCGCCAA GGAGCTCTGC CTTCCTCCCG TGAAACTGCA CTGCTCCAAA 
TCTGTGCTGT TTCCAGCAGA GGAGAAAACT CAGCTTTCGC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001306971.1
CDS	1161..1625
Translation	MAAAGAFRLRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSKSVLFPAEEKTQLSP

Target ORF information:

RefSeq Version	NM_001320042.1
Organism	Homo sapiens(human)
Definition	Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320042.1

ORF Insert Sequence:

ATGGCGGCGG CTGGGGCTTT CCGTCTGAGG CGGGCGGCAT CGGCTCTGCT GCTGCGGAGC 
CCCCGCCTGC CCGCCCGGGA GCTGTCGGCC CCGGCCCGAC TCTATCACAA GAAGGTTGTT 
GATCATTATG AAAATCCTAG AAACGTGGGG TCCCTTGACA AGACATCTAA AAATGTTGGA 
ACTGGACTGG TGGGGGCTCC AGCATGTGGT GACGTAATGA AATTACAGAT TCAAGTGGAT 
GAAAAGGGGA AGATTGTGGA TGCTAGGTTT AAAACATTTG GCTGTGGTTC CGCAATTGCC 
TCCAGCTCAT TAGCCACTGA ATGGGTGAAA GGAAAGACGG TGGAGGAAGC CTTGACTATC 
AAAAACACAG ATATCGCCAA GGAGCTCTGC CTTCCTCCCG TGAAACTGCA CTGCTCCAAA 
TCTGTGCTGT TTCCAGCAGA GGAGAAAACT CAGCTTTCGC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISCU ( NM_001301141.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001288070.1 Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant 4, mRNA.

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CloneID	OHu46445
Clone ID Related Accession (Same CDS sequence)	NM_001301141.1
Accession Version	NM_001301141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK057251.1, AC008119.6 and BQ020993.1. On Jul 23, 2014 this sequence version replaced XM_005268760.1. Transcript Variant: This variant (4) contains an alternate exon in the 3' region, resulting in a different 3' coding region and 3' UTR, compared to variant 2. The encoded isoform (4) has a distinct, shorter C-terminus, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK057251.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CTGGGGCTTT CCGTCTGAGG CGGGCGGCAT CGGCTCTGCT GCTGCGGAGC 
CCCCGCCTGC CCGCCCGGGA GCTGTCGGCC CCGGCCCGAC TCTATCACAA GAAGGTTGTT 
GATCATTATG AAAATCCTAG AAACGTGGGG TCCCTTGACA AGACATCTAA AAATGTTGGA 
ACTGGACTGG TGGGGGCTCC AGCATGTGGT GACGTAATGA AATTACAGAT TCAAGTGGAT 
GAAAAGGGGA AGATTGTGGA TGCTAGGTTT AAAACATTTG GCTGTGGTTC CGCAATTGCC 
TCCAGCTCAT TAGCCACTGA ATGGGTGAAA GGAAAGACGG TGGAGGAAGC CTTGACTATC 
AAAAACACAG ATATCGCCAA GGAGCTCTGC CTTCCTCCCG TGAAACTGCA CTGCTCCAGG 
AAAGAAGGAA TGAGAAACAT TAGCCTTAAT GCATCGATGG AGGTTTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288070.1
CDS	1161..1631
Translation	MAAAGAFRLRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSRKEGMRNISLNASMEVY

Target ORF information:

RefSeq Version	NM_001301141.1
Organism	Homo sapiens(human)
Definition	Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301141.1

ORF Insert Sequence:

ATGGCGGCGG CTGGGGCTTT CCGTCTGAGG CGGGCGGCAT CGGCTCTGCT GCTGCGGAGC 
CCCCGCCTGC CCGCCCGGGA GCTGTCGGCC CCGGCCCGAC TCTATCACAA GAAGGTTGTT 
GATCATTATG AAAATCCTAG AAACGTGGGG TCCCTTGACA AGACATCTAA AAATGTTGGA 
ACTGGACTGG TGGGGGCTCC AGCATGTGGT GACGTAATGA AATTACAGAT TCAAGTGGAT 
GAAAAGGGGA AGATTGTGGA TGCTAGGTTT AAAACATTTG GCTGTGGTTC CGCAATTGCC 
TCCAGCTCAT TAGCCACTGA ATGGGTGAAA GGAAAGACGG TGGAGGAAGC CTTGACTATC 
AAAAACACAG ATATCGCCAA GGAGCTCTGC CTTCCTCCCG TGAAACTGCA CTGCTCCAGG 
AAAGAAGGAA TGAGAAACAT TAGCCTTAAT GCATCGATGG AGGTTTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISCU ( XM_011538100.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536402.1 Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant X1, mRNA.

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CloneID	OHu46447
Clone ID Related Accession (Same CDS sequence)	XM_011538100.2
Accession Version	XM_011538100.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538100.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCTCA TTGACATGAG TGTAGACCTT TCTACTCAGG TTGTTGATCA TTATGAAAAT 
CCTAGAAACG TGGGGTCCCT TGACAAGACA TCTAAAAATG TTGGAACTGG ACTGGTGGGG 
GCTCCAGCAT GTGGTGACGT AATGAAATTA CAGATTCAAG TGGATGAAAA GGGGAAGATT 
GTGGATGCTA GGTTTAAAAC ATTTGGCTGT GGTTCCGCAA TTGCCTCCAG CTCATTAGCC 
ACTGAATGGG TGAAAGGAAA GACGGTGGAG GAAGCCTTGA CTATCAAAAA CACAGATATC 
GCCAAGGAGC TCTGCCTTCC TCCCGTGAAA CTGCACTGCT CCAAATCTGT GCTGTTTCCA 
GCAGAGGAGA AAACTCAGCT TTCGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536402.1
CDS	1332..1721
Translation	MVLIDMSVDLSTQVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSKSVLFPAEEKTQLSP

Target ORF information:

RefSeq Version	XM_011538100.2
Organism	Homo sapiens(human)
Definition	Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538100.2

ORF Insert Sequence:

ATGGTTCTCA TTGACATGAG TGTAGACCTT TCTACTCAGG TTGTTGATCA TTATGAAAAT 
CCTAGAAACG TGGGGTCCCT TGACAAGACA TCTAAAAATG TTGGAACTGG ACTGGTGGGG 
GCTCCAGCAT GTGGTGACGT AATGAAATTA CAGATTCAAG TGGATGAAAA GGGGAAGATT 
GTGGATGCTA GGTTTAAAAC ATTTGGCTGT GGTTCCGCAA TTGCCTCCAG CTCATTAGCC 
ACTGAATGGG TGAAAGGAAA GACGGTGGAG GAAGCCTTGA CTATCAAAAA CACAGATATC 
GCCAAGGAGC TCTGCCTTCC TCCCGTGAAA CTGCACTGCT CCAAATCTGT GCTGTTTCCA 
GCAGAGGAGA AAACTCAGCT TTCGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISCU ( XM_011538101.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536403.1 Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu66215
Clone ID Related Accession (Same CDS sequence)	XM_011538101.2
Accession Version	XM_011538101.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	iron-sulfur cluster assembly enzyme ISCU, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538101.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CTGGGGCTTT CCGTCTGAGG CGGGCGGCAT CGGCTCTGCT GCTGCGGAGC 
CCCCGCCTGC CCGCCCGGGA GCTGTCGGCC CCGGCCCGAC TCTATCACAA GAAGGTTGTT 
GATCATTATG AAAATCCTAG AAACGTGGGG TCCCTTGACA AGACATCTAA AAATGTTGGA 
ACTGGACTGG TGGGGGCTCC AGCATGTGGT GACGTAATGA AATTACAGAT TCAAGTGGAT 
GAAAAGGGGA AGATTGTGGA TGCTAGGTTT AAAACATTTG GCTGTGGTTC CGCAATTGCC 
TCCAGCTCAT TAGCCACTGA ATGGGTGAAA GGAAAGACGT GCTGGCTGAA GATGCAATCA 
AGGCCGCCCT GGCTGATTAC AAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536403.1
CDS	1161..1547
Translation	MAAAGAFRLRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTCWLKMQSRPPWLITN

Target ORF information:

RefSeq Version	XM_011538101.2
Organism	Homo sapiens(human)
Definition	Homo sapiens iron-sulfur cluster assembly enzyme (ISCU), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538101.2

ORF Insert Sequence:

ATGGCGGCGG CTGGGGCTTT CCGTCTGAGG CGGGCGGCAT CGGCTCTGCT GCTGCGGAGC 
CCCCGCCTGC CCGCCCGGGA GCTGTCGGCC CCGGCCCGAC TCTATCACAA GAAGGTTGTT 
GATCATTATG AAAATCCTAG AAACGTGGGG TCCCTTGACA AGACATCTAA AAATGTTGGA 
ACTGGACTGG TGGGGGCTCC AGCATGTGGT GACGTAATGA AATTACAGAT TCAAGTGGAT 
GAAAAGGGGA AGATTGTGGA TGCTAGGTTT AAAACATTTG GCTGTGGTTC CGCAATTGCC 
TCCAGCTCAT TAGCCACTGA ATGGGTGAAA GGAAAGACGT GCTGGCTGAA GATGCAATCA 
AGGCCGCCCT GGCTGATTAC AAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Iron-sulfur cluster biogenesis in mammalian cells: New insights into the molecular mechanisms of cluster delivery.
Biochimica et biophysica acta1853(6)1493-512(2015 Jun)
Maio N,Rouault TA

Tissue specificity of a human mitochondrial disease: differentiation-enhanced mis-splicing of the Fe-S scaffold gene ISCU renders patient cells more sensitive to oxidative stress in ISCU myopathy.
The Journal of biological chemistry287(48)40119-30(2012 Nov)
Crooks DR,Jeong SY,Tong WH,Ghosh MC,Olivierre H,Haller RG,Rouault TA

Tissue-specific splicing of ISCU results in a skeletal muscle phenotype in myopathy with lactic acidosis, while complete loss of ISCU results in early embryonic death in mice.
Human genetics129(4)371-8(2011 Apr)
Nordin A,Larsson E,Thornell LE,Holmberg M

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ISCU cDNA ORF clone, Homo sapiens(human)

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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