HLA-DOA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HLA-DOA
Entrez Gene ID	3111
Full Name	major histocompatibility complex, class II, DO alpha
Synonyms	HLA-DNA,HLA-DZA,HLADZ
General protein information	Preferred Names major histocompatibility complex, class II, DO alpha Names HLA class II histocompatibility antigen, DO alpha chain HLA-D0-alpha MHC DN-alpha MHC DZ alpha MHC class II antigen DOA lymphocyte antigen major histocompatibility complex, class II, DN alpha
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.32
Summary	HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. [provided by RefSeq, Jul 2008].
Disorder MIM:	142930

mRNA and Protein(s)

mRNA	Protein	Name
NM_002119.3	NP_002110.1	HLA class II histocompatibility antigen, DO alpha chain precursor
NM_002119.4	NP_002110.1	HLA class II histocompatibility antigen, DO alpha chain precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	HLA-DOA	NP_002110.1
Pan troglodytes (chimpanzee)	LOC471979	XP_527358.2
	MAMU-DOA	XP_001115887.1
Bos taurus (cattle)	BOLA-DOA	NP_001192849.1
Canis lupus familiaris (dog)	DLA-DOA	XP_532104.3
Mus musculus (house mouse)	H2-Oa	NP_032232.2
Rattus norvegicus (Norway rat)	RT1-DOa	NP_898874.2

Gene Ontology
Bibliography

Related articles in PubMed

Contribution of a Non-classical HLA Gene, HLA-DOA, to the Risk of Rheumatoid Arthritis.
Okada Y, Suzuki A, Ikari K, Terao C, Kochi Y, Ohmura K, Higasa K, Akiyama M, Ashikawa K, Kanai M, Hirata J, Suita N, Teo YY, Xu H, Bae SC, Takahashi A, Momozawa Y, Matsuda K, Momohara S, Taniguchi A, Yamada R, Mimori T, Kubo M, Brown MA, Raychaudhuri S, Matsuda F, Yamanaka H, Kamatani Y, Yamamoto K
American journal of human genetics99(2)366-74(2016 Aug)

Genome-wide association analysis on five isolated populations identifies variants of the HLA-DOA gene associated with white wine liking.
Pirastu N, Kooyman M, Traglia M, Robino A, Willems SM, Pistis G, Amin N, Sala C, Karssen LC, van Duijn CM, Toniolo D, Gasparini P
European journal of human genetics : EJHG23(12)1717-22(2015 Dec)

pH-susceptibility of HLA-DO tunes DO/DM ratios to regulate HLA-DM catalytic activity.
Jiang W, Strohman MJ, Somasundaram S, Ayyangar S, Hou T, Wang N, Mellins ED
Scientific reports517333(2015 Nov)

Association of polymorphisms in HLA antigen presentation-related genes with the outcomes of HCV infection.
Huang P, Zhang Y, Lu X, Xu Y, Wang J, Zhang Y, Yu R, Su J
PloS one10(4)e0123513(2015)

Human leukocyte antigen-DO regulates surface presentation of human leukocyte antigen class II-restricted antigens on B cell malignancies.
Kremer AN, van der Meijden ED, Honders MW, Pont MJ, Goeman JJ, Falkenburg JH, Griffioen M
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation20(5)742-7(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Contribution of a Non-classical HLA Gene, HLA-DOA, to the Risk of Rheumatoid Arthritis
Title: Contribution of a Non-classical HLA Gene, HLA-DOA, to the Risk of Rheumatoid Arthritis.

significant association was found between white wine liking and rs9276975:C>T polymorphism in the HLA-DOA gene.
Title: Genome-wide association analysis on five isolated populations identifies variants of the HLA-DOA gene associated with white wine liking.

pH-susceptibility of HLA-DO tunes DO/DM ratios to regulate HLA-DM catalytic activity.
Title: pH-susceptibility of HLA-DO tunes DO/DM ratios to regulate HLA-DM catalytic activity.

TAP2, HLA-DOA, HLA-DOB, and tapasin loci are novel candidate regions for susceptibility to HCV infection and viral clearance in the Chinese population
Title: Association of polymorphisms in HLA antigen presentation-related genes with the outcomes of HCV infection.

HLA-DO regulates surface presentation of human leukocyte antigen class II-restricted antigens on B cell malignancies.
Title: Human leukocyte antigen-DO regulates surface presentation of human leukocyte antigen class II-restricted antigens on B cell malignancies.

The following HLA-DOA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLA-DOA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19046	NM_002119.4 Latest version!	Homo sapiens major histocompatibility complex, class II, DO alpha (HLA-DOA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu19046	NM_002119.3	Homo sapiens major histocompatibility complex, class II, DO alpha (HLA-DOA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HLA-DOA ( NM_002119.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002110.1 Homo sapiens major histocompatibility complex, class II, DO alpha (HLA-DOA), mRNA.

Price & Availability↑

CloneID	OHu19046
Clone ID Related Accession (Same CDS sequence)	NM_002119.3 , NM_002119.4
Accession Version	NM_002119.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	HLA class II histocompatibility antigen, DO alpha chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M31525.1 and AL645931.7. This sequence is a reference standard in the RefSeqGene project. On or before Apr 27, 2005 this sequence version replaced NM_015851.1, NM_002119.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M31525.1, SRR1803612.110203.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTCA GAGCAGGGCT GGTCCTGGGG TTCCACACCC TGATGACCCT CCTGAGCCCG 
CAGGAGGCAG GGGCCACCAA GGCTGACCAC ATGGGCTCCT ACGGACCCGC CTTCTACCAG 
TCTTACGGCG CCTCGGGCCA GTTCACCCAT GAATTTGATG AGGAACAGCT GTTCTCTGTG 
GACCTGAAGA AAAGCGAGGC CGTGTGGCGT CTGCCTGAGT TTGGTGACTT TGCCCGCTTT 
GACCCGCAGG GCGGGCTGGC CGGCATCGCC GCAATCAAAG CCCATCTGGA CATCCTGGTG 
GAGCGCTCCA ACCGCAGCAG AGCCATCAAC GTGCCTCCAC GGGTGACCGT GCTCCCCAAG 
TCTCGGGTGG AGCTGGGCCA GCCCAACATC CTCATCTGCA TCGTGGACAA CATCTTCCCC 
CCTGTGATCA ATATCACCTG GCTGCGCAAC GGCCAAACTG TCACTGAGGG AGTGGCCCAG 
ACCAGCTTCT ATTCCCAGCC TGACCATTTG TTCCGCAAGT TCCACTACCT GCCCTTCGTG 
CCCTCAGCCG AGGACGTCTA TGACTGCCAG GTGGAGCACT GGGGCCTGGA TGCGCCACTC 
CTCAGGCATT GGGAGCTCCA GGTGCCTATT CCACCACCAG ATGCCATGGA GACCCTGGTC 
TGTGCCCTGG GCCTGGCCAT CGGCCTGGTG GGCTTCCTCG TGGGCACCGT CCTCATCATC 
ATGGGCACAT ATGTGTCCAG TGTCCCCAGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002110.1
CDS	77..829
Translation	MALRAGLVLGFHTLMTLLSPQEAGATKADHMGSYGPAFYQSYGASGQFTHEFDEEQLFSVDLKKSEAVWRLPEFGDFARFDPQGGLAGIAAIKAHLDILVERSNRSRAINVPPRVTVLPKSRVELGQPNILICIVDNIFPPVINITWLRNGQTVTEGVAQTSFYSQPDHLFRKFHYLPFVPSAEDVYDCQVEHWGLDAPLLRHWELQVPIPPPDAMETLVCALGLAIGLVGFLVGTVLIIMGTYVSSVPR

Target ORF information:

RefSeq Version	NM_002119.3
Organism	Homo sapiens(human)
Definition	Homo sapiens major histocompatibility complex, class II, DO alpha (HLA-DOA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002119.3

ORF Insert Sequence:

ATGGCCCTCA GAGCAGGGCT GGTCCTGGGG TTCCACACCC TGATGACCCT CCTGAGCCCG 
CAGGAGGCAG GGGCCACCAA GGCTGACCAC ATGGGCTCCT ACGGACCCGC CTTCTACCAG 
TCTTACGGCG CCTCGGGCCA GTTCACCCAT GAATTTGATG AGGAACAGCT GTTCTCTGTG 
GACCTGAAGA AAAGCGAGGC CGTGTGGCGT CTGCCTGAGT TTGGTGACTT TGCCCGCTTT 
GACCCGCAGG GCGGGCTGGC CGGCATCGCC GCAATCAAAG CCCATCTGGA CATCCTGGTG 
GAGCGCTCCA ACCGCAGCAG AGCCATCAAC GTGCCTCCAC GGGTGACCGT GCTCCCCAAG 
TCTCGGGTGG AGCTGGGCCA GCCCAACATC CTCATCTGCA TCGTGGACAA CATCTTCCCC 
CCTGTGATCA ATATCACCTG GCTGCGCAAC GGCCAAACTG TCACTGAGGG AGTGGCCCAG 
ACCAGCTTCT ATTCCCAGCC TGACCATTTG TTCCGCAAGT TCCACTACCT GCCCTTCGTG 
CCCTCAGCCG AGGACGTCTA TGACTGCCAG GTGGAGCACT GGGGCCTGGA TGCGCCACTC 
CTCAGGCATT GGGAGCTCCA GGTGCCTATT CCACCACCAG ATGCCATGGA GACCCTGGTC 
TGTGCCCTGG GCCTGGCCAT CGGCCTGGTG GGCTTCCTCG TGGGCACCGT CCTCATCATC 
ATGGGCACAT ATGTGTCCAG TGTCCCCAGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLA-DOA ( NM_002119.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002110.1 Homo sapiens major histocompatibility complex, class II, DO alpha (HLA-DOA), mRNA.

Price & Availability↑

CloneID	OHu19046
Clone ID Related Accession (Same CDS sequence)	NM_002119.3 , NM_002119.4
Accession Version	NM_002119.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	HLA class II histocompatibility antigen, DO alpha chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M31525.1 and AL645931.7. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_002119.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.110203.1, BC013183.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000229829.7/ ENSP00000229829.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCCTCA GAGCAGGGCT GGTCCTGGGG TTCCACACCC TGATGACCCT CCTGAGCCCG 
CAGGAGGCAG GGGCCACCAA GGCTGACCAC ATGGGCTCCT ACGGACCCGC CTTCTACCAG 
TCTTACGGCG CCTCGGGCCA GTTCACCCAT GAATTTGATG AGGAACAGCT GTTCTCTGTG 
GACCTGAAGA AAAGCGAGGC CGTGTGGCGT CTGCCTGAGT TTGGTGACTT TGCCCGCTTT 
GACCCGCAGG GCGGGCTGGC CGGCATCGCC GCAATCAAAG CCCATCTGGA CATCCTGGTG 
GAGCGCTCCA ACCGCAGCAG AGCCATCAAC GTGCCTCCAC GGGTGACCGT GCTCCCCAAG 
TCTCGGGTGG AGCTGGGCCA GCCCAACATC CTCATCTGCA TCGTGGACAA CATCTTCCCC 
CCTGTGATCA ATATCACCTG GCTGCGCAAC GGCCAAACTG TCACTGAGGG AGTGGCCCAG 
ACCAGCTTCT ATTCCCAGCC TGACCATTTG TTCCGCAAGT TCCACTACCT GCCCTTCGTG 
CCCTCAGCCG AGGACGTCTA TGACTGCCAG GTGGAGCACT GGGGCCTGGA TGCGCCACTC 
CTCAGGCATT GGGAGCTCCA GGTGCCTATT CCACCACCAG ATGCCATGGA GACCCTGGTC 
TGTGCCCTGG GCCTGGCCAT CGGCCTGGTG GGCTTCCTCG TGGGCACCGT CCTCATCATC 
ATGGGCACAT ATGTGTCCAG TGTCCCCAGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002110.1
CDS	56..808
Translation	MALRAGLVLGFHTLMTLLSPQEAGATKADHMGSYGPAFYQSYGASGQFTHEFDEEQLFSVDLKKSEAVWRLPEFGDFARFDPQGGLAGIAAIKAHLDILVERSNRSRAINVPPRVTVLPKSRVELGQPNILICIVDNIFPPVINITWLRNGQTVTEGVAQTSFYSQPDHLFRKFHYLPFVPSAEDVYDCQVEHWGLDAPLLRHWELQVPIPPPDAMETLVCALGLAIGLVGFLVGTVLIIMGTYVSSVPR

Target ORF information:

RefSeq Version	NM_002119.4
Organism	Homo sapiens(human)
Definition	Homo sapiens major histocompatibility complex, class II, DO alpha (HLA-DOA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002119.4

ORF Insert Sequence:

ATGGCCCTCA GAGCAGGGCT GGTCCTGGGG TTCCACACCC TGATGACCCT CCTGAGCCCG 
CAGGAGGCAG GGGCCACCAA GGCTGACCAC ATGGGCTCCT ACGGACCCGC CTTCTACCAG 
TCTTACGGCG CCTCGGGCCA GTTCACCCAT GAATTTGATG AGGAACAGCT GTTCTCTGTG 
GACCTGAAGA AAAGCGAGGC CGTGTGGCGT CTGCCTGAGT TTGGTGACTT TGCCCGCTTT 
GACCCGCAGG GCGGGCTGGC CGGCATCGCC GCAATCAAAG CCCATCTGGA CATCCTGGTG 
GAGCGCTCCA ACCGCAGCAG AGCCATCAAC GTGCCTCCAC GGGTGACCGT GCTCCCCAAG 
TCTCGGGTGG AGCTGGGCCA GCCCAACATC CTCATCTGCA TCGTGGACAA CATCTTCCCC 
CCTGTGATCA ATATCACCTG GCTGCGCAAC GGCCAAACTG TCACTGAGGG AGTGGCCCAG 
ACCAGCTTCT ATTCCCAGCC TGACCATTTG TTCCGCAAGT TCCACTACCT GCCCTTCGTG 
CCCTCAGCCG AGGACGTCTA TGACTGCCAG GTGGAGCACT GGGGCCTGGA TGCGCCACTC 
CTCAGGCATT GGGAGCTCCA GGTGCCTATT CCACCACCAG ATGCCATGGA GACCCTGGTC 
TGTGCCCTGG GCCTGGCCAT CGGCCTGGTG GGCTTCCTCG TGGGCACCGT CCTCATCATC 
ATGGGCACAT ATGTGTCCAG TGTCCCCAGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Contribution of a Non-classical HLA Gene, HLA-DOA, to the Risk of Rheumatoid Arthritis.
American journal of human genetics99(2)366-74(2016 Aug)
Okada Y,Suzuki A,Ikari K,Terao C,Kochi Y,Ohmura K,Higasa K,Akiyama M,Ashikawa K,Kanai M,Hirata J,Suita N,Teo YY,Xu H,Bae SC,Takahashi A,Momozawa Y,Matsuda K,Momohara S,Taniguchi A,Yamada R,Mimori T,Kubo M,Brown MA,Raychaudhuri S,Matsuda F,Yamanaka H,Kamatani Y,Yamamoto K

Genome-wide association analysis on five isolated populations identifies variants of the HLA-DOA gene associated with white wine liking.
European journal of human genetics : EJHG23(12)1717-22(2015 Dec)
Pirastu N,Kooyman M,Traglia M,Robino A,Willems SM,Pistis G,Amin N,Sala C,Karssen LC,van Duijn CM,Toniolo D,Gasparini P

pH-susceptibility of HLA-DO tunes DO/DM ratios to regulate HLA-DM catalytic activity.
Scientific reports517333(2015 Nov)
Jiang W,Strohman MJ,Somasundaram S,Ayyangar S,Hou T,Wang N,Mellins ED

Association of polymorphisms in HLA antigen presentation-related genes with the outcomes of HCV infection.
PloS one10(4)e0123513(2015)
Huang P,Zhang Y,Lu X,Xu Y,Wang J,Zhang Y,Yu R,Su J

Human leukocyte antigen-DO regulates surface presentation of human leukocyte antigen class II-restricted antigens on B cell malignancies.
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation20(5)742-7(2014 May)
Kremer AN,van der Meijden ED,Honders MW,Pont MJ,Goeman JJ,Falkenburg JH,Griffioen M

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HLA-DOA cDNA ORF clone, Homo sapiens(human)

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Related articles in PubMed

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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