×

KRT86 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol KRT86
Entrez Gene ID 3892
Full Name keratin 86
Synonyms HB6,Hb1,K86,KRTHB1,KRTHB6,MNX
General protein information
Preferred Names

keratin 86

Names

keratin, type II cuticular Hb6
hair keratin K2.11
hard keratin, type II, 6
keratin 86, type II
keratin protein HB6
keratin, hair, basic, 6 (monilethrix)
type II hair keratin Hb6
type-II keratin Kb26

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

12

12q13.13

Disorder MIM:

601928

Disorder Html: Monilethrix, 158000 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_017019296.1 XP_016874785.1 keratin, type II cuticular Hb6 isoform X2
NM_001320198.1 NP_001307127.1 keratin, type II cuticular Hb6
XM_005268866.4 XP_005268923.1 keratin, type II cuticular Hb6 isoform X1



Canis lupus familiaris (dog) KRT86 XP_543656.2
Homo sapiens (human) KRT86 NP_002275.1
Pan troglodytes (chimpanzee) KRT86 XP_522385.2
Mus musculus (house mouse) Krt86 NP_034797.1
Rattus norvegicus (Norway rat) Krt86 NP_001008810.1
Bos taurus (cattle) KRT86 NP_001179997.1


Related articles in PubMed

A novel KRT86 mutation in a Turkish family with monilethrix, and identification of maternal mosaicism.
Redler S, Pasternack SM, Wolf S, Stienen D, Wenzel J, Nöthen MM, Betz RC
Clinical and experimental dermatology40(7)781-5(2015 Oct)

Novel KRT83 and KRT86 mutations associated with monilethrix.
van Steensel M, Vreeburg M, Urbina MT, López P, Morice-Picard F, van Geel M
Experimental dermatology24(3)222-4(2015 Mar)

Mutation detection of type II hair cortex keratin gene KRT86 in a Chinese Han family with congenital monilethrix.
Ye ZZ, Nan X, Zhao HS, Chen XR, Song QH
Chinese medical journal126(16)3103-6(2013 Aug)

Congenital monilethrix and hereditary unilateral external auditory canal atresia are co-inherited in a Chinese pedigree with recurrent KRT86 mutation.
Feng YG, Xiao SX, Xu AL, Feng JY, Wang JM
The Journal of dermatology39(9)817-9(2012 Sep)

A novel monilethrix mutation in coil 2A of KRT86 causing autosomal dominant monilethrix with incomplete penetrance.
De Cruz R, Horev L, Green J, Babay S, Sladden M, Zlotogorski A, Sinclair R
The British journal of dermatology166 Suppl 220-6(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Analysis of KRT86 revealed a novel mutation, c.1231G>T;p.Glu411*, in exon 7 in the three affected children and their mother with monilethrix.
Title: A novel KRT86 mutation in a Turkish family with monilethrix, and identification of maternal mosaicism.

Novel mutations causing monilethrix are reported in KRT81, KRT83, and KRT86.
Title: Novel KRT83 and KRT86 mutations associated with monilethrix.

The mutation of extron 7 of KRT86 identified plays a major role in the pathogenesis of this pedigree with monilethrix.
Title: Mutation detection of type II hair cortex keratin gene KRT86 in a Chinese Han family with congenital monilethrix.

Congenital monilethrix and hereditary unilateral external auditory canal atresia are co-inherited in a Chinese pedigree with recurrent KRT86 mutation.
Title: Congenital monilethrix and hereditary unilateral external auditory canal atresia are co-inherited in a Chinese pedigree with recurrent KRT86 mutation.

Results idntify a novel mutation of krt86 protein causing monilethrix located in a region other than the helix.
Title: A novel monilethrix mutation in coil 2A of KRT86 causing autosomal dominant monilethrix with incomplete penetrance.

The following KRT86 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KRT86 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu17915 NM_001320198.1
Latest version!
Homo sapiens keratin 86 (KRT86), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu17915 XM_017019296.1
Latest version!
Homo sapiens keratin 86 (KRT86), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu46550 XM_005268866.4
Latest version!
Homo sapiens keratin 86 (KRT86), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu17915
Clone ID Related Accession (Same CDS sequence) XM_017019296.1 , NM_001320198.1
Accession Version NM_001320198.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1461bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-10-09
Organism Homo sapiens(Human)
Product keratin, type II cuticular Hb6
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC399862.1, AK292237.1, BC069585.1, BG216771.1 and AI368926.1. This sequence is a reference standard in the RefSeqGene project. On Feb 12, 2016 this sequence version replaced XM_011538337.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292237.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACTTGTG GATCTTACTG TGGTGGCCGC GCCTTCAGCT GCATCTCGGC CTGCGGGCCC 
CGGCCCGGCC GCTGCTGCAT CACCGCCGCC CCCTACCGTG GCATCTCCTG CTACCGCGGC
CTCACCGGGG GCTTCGGCAG CCACAGCGTG TGCGGAGGCT TTCGGGCCGG CTCCTGCGGA
CGCAGCTTCG GCTACCGCTC CGGGGGCGTG TGCGGGCCCA GTCCCCCATG CATCACCACC
GTGTCGGTCA ACGAGAGCCT CCTCACGCCC CTCAACCTGG AGATCGACCC CAACGCGCAG
TGCGTGAAGC AGGAGGAGAA GGAGCAGATC AAGTCCCTCA ACAGCAGGTT CGCGGCCTTC
ATCGACAAGG TGCGCTTCCT GGAGCAGCAG AACAAACTGC TGGAGACAAA GCTGCAGTTC
TACCAGAACC GCGAGTGTTG CCAGAGCAAC CTGGAGCCCC TGTTTGAGGG CTACATCGAG
ACTCTGCGGC GGGAGGCCGA GTGCGTGGAG GCCGACAGCG GGAGGCTGGC CTCAGAGCTT
AACCACGTGC AGGAGGTGCT GGAGGGCTAC AAGAAGAAGT ATGAGGAGGA GGTTTCTCTG
AGAGCAACAG CTGAGAACGA GTTTGTGGCT CTGAAGAAGG ATGTGGACTG CGCCTACCTC
CGCAAATCAG ACCTGGAGGC CAATGTGGAG GCCCTGATCC AGGAGATCGA CTTCCTGAGG
CGGCTGTATG AGGAGGAGAT CCGCGTTCTC CAGTCCCACA TCTCAGACAC CTCCGTGGTT
GTCAAGCTGG ACAACAGCCG GGACCTGAAC ATGGACTGCA TCATTGCCGA GATCAAGGCA
CAGTACGATG ACATTGTCAC CCGTAGCCGG GCTGAGGCCG AGTCCTGGTA CCGCAGCAAG
TGTGAGGAGA TGAAGGCCAC GGTGATCAGG CACGGGGAGA CCCTGCGCCG CACCAAGGAG
GAGATCAACG AGCTGAACCG CATGATCCAG AGGCTGACGG CTGAGGTGGA GAATGCCAAG
TGCCAGAATT CCAAGCTGGA GGCTGCGGTG GCTCAGTCTG AGCAGCAGGG TGAGGCGGCC
CTCAGCGATG CCCGCTGCAA GTTGGCCGAG CTGGAGGGTG CCCTGCAGAA GGCCAAGCAG
GACATGGCCT GCCTGATCAG GGAGTACCAG GAGGTGATGA ACTCCAAGCT GGGCCTGGAC
ATCGAGATCG CCACCTACAG GCGCCTGCTG GAGGGCGAGG AGCAGAGGCT GTGCGAGGGC
GTCGGCTCGG TGAATGTCTG CGTCAGCAGC TCCCGCGGTG GCGTTGTCTG TGGCGATCTC
TGCGCCTCCA CTACTGCCCC TGTTGTCTCC ACCAGAGTCA GTAGCGTCCC CAGCAACAGC
AACGTGGTGG TGGGCACTAC TAACGCCTGC GCCCCCTCCG CCCGGGTTGG CGTCTGCGGC
GGCAGCTGTA AGAGGTGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001307127.1
CDS209..1669
Misc Feature(1)173..175
Misc Feature(2)209..526
Misc Feature(3)<215..514
Misc Feature(4)521..1456
Misc Feature(5)527..1447
Misc Feature(6)527..631
Misc Feature(7)632..661
Misc Feature(8)662..964
Misc Feature(9)881..1228
Misc Feature(10)965..1015
Misc Feature(11)1016..1447
Misc Feature(12)<1151..>1357
Misc Feature(13)1448..1666
Exon (1)1..78
Exon (2)79..204
Exon (3)205..577
Exon (4)578..786
Exon (5)787..847
Exon (6)848..943
Exon (7)944..1108
Exon (8)1109..1234
Exon (9)1235..1455
Exon (10)1456..1487
Exon (11)1488..2247
Translation

Target ORF information:

RefSeq Version NM_001320198.1
Organism Homo sapiens(Human)
Definition Homo sapiens keratin 86 (KRT86), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001320198.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACTTGTG GATCTTACTG TGGTGGCCGC GCCTTCAGCT GCATCTCGGC CTGCGGGCCC 
CGGCCCGGCC GCTGCTGCAT CACCGCCGCC CCCTACCGTG GCATCTCCTG CTACCGCGGC
CTCACCGGGG GCTTCGGCAG CCACAGCGTG TGCGGAGGCT TTCGGGCCGG CTCCTGCGGA
CGCAGCTTCG GCTACCGCTC CGGGGGCGTG TGCGGGCCCA GTCCCCCATG CATCACCACC
GTGTCGGTCA ACGAGAGCCT CCTCACGCCC CTCAACCTGG AGATCGACCC CAACGCGCAG
TGCGTGAAGC AGGAGGAGAA GGAGCAGATC AAGTCCCTCA ACAGCAGGTT CGCGGCCTTC
ATCGACAAGG TGCGCTTCCT GGAGCAGCAG AACAAACTGC TGGAGACAAA GCTGCAGTTC
TACCAGAACC GCGAGTGTTG CCAGAGCAAC CTGGAGCCCC TGTTTGAGGG CTACATCGAG
ACTCTGCGGC GGGAGGCCGA GTGCGTGGAG GCCGACAGCG GGAGGCTGGC CTCAGAGCTT
AACCACGTGC AGGAGGTGCT GGAGGGCTAC AAGAAGAAGT ATGAGGAGGA GGTTTCTCTG
AGAGCAACAG CTGAGAACGA GTTTGTGGCT CTGAAGAAGG ATGTGGACTG CGCCTACCTC
CGCAAATCAG ACCTGGAGGC CAATGTGGAG GCCCTGATCC AGGAGATCGA CTTCCTGAGG
CGGCTGTATG AGGAGGAGAT CCGCGTTCTC CAGTCCCACA TCTCAGACAC CTCCGTGGTT
GTCAAGCTGG ACAACAGCCG GGACCTGAAC ATGGACTGCA TCATTGCCGA GATCAAGGCA
CAGTACGATG ACATTGTCAC CCGTAGCCGG GCTGAGGCCG AGTCCTGGTA CCGCAGCAAG
TGTGAGGAGA TGAAGGCCAC GGTGATCAGG CACGGGGAGA CCCTGCGCCG CACCAAGGAG
GAGATCAACG AGCTGAACCG CATGATCCAG AGGCTGACGG CTGAGGTGGA GAATGCCAAG
TGCCAGAATT CCAAGCTGGA GGCTGCGGTG GCTCAGTCTG AGCAGCAGGG TGAGGCGGCC
CTCAGCGATG CCCGCTGCAA GTTGGCCGAG CTGGAGGGTG CCCTGCAGAA GGCCAAGCAG
GACATGGCCT GCCTGATCAG GGAGTACCAG GAGGTGATGA ACTCCAAGCT GGGCCTGGAC
ATCGAGATCG CCACCTACAG GCGCCTGCTG GAGGGCGAGG AGCAGAGGCT GTGCGAGGGC
GTCGGCTCGG TGAATGTCTG CGTCAGCAGC TCCCGCGGTG GCGTTGTCTG TGGCGATCTC
TGCGCCTCCA CTACTGCCCC TGTTGTCTCC ACCAGAGTCA GTAGCGTCCC CAGCAACAGC
AACGTGGTGG TGGGCACTAC TAACGCCTGC GCCCCCTCCG CCCGGGTTGG CGTCTGCGGC
GGCAGCTGTA AGAGGTGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17915
Clone ID Related Accession (Same CDS sequence) XM_017019296.1 , NM_001320198.1
Accession Version XM_017019296.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1461bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product keratin, type II cuticular Hb6 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACTTGTG GATCTTACTG TGGTGGCCGC GCCTTCAGCT GCATCTCGGC CTGCGGGCCC 
CGGCCCGGCC GCTGCTGCAT CACCGCCGCC CCCTACCGTG GCATCTCCTG CTACCGCGGC
CTCACCGGGG GCTTCGGCAG CCACAGCGTG TGCGGAGGCT TTCGGGCCGG CTCCTGCGGA
CGCAGCTTCG GCTACCGCTC CGGGGGCGTG TGCGGGCCCA GTCCCCCATG CATCACCACC
GTGTCGGTCA ACGAGAGCCT CCTCACGCCC CTCAACCTGG AGATCGACCC CAACGCGCAG
TGCGTGAAGC AGGAGGAGAA GGAGCAGATC AAGTCCCTCA ACAGCAGGTT CGCGGCCTTC
ATCGACAAGG TGCGCTTCCT GGAGCAGCAG AACAAACTGC TGGAGACAAA GCTGCAGTTC
TACCAGAACC GCGAGTGTTG CCAGAGCAAC CTGGAGCCCC TGTTTGAGGG CTACATCGAG
ACTCTGCGGC GGGAGGCCGA GTGCGTGGAG GCCGACAGCG GGAGGCTGGC CTCAGAGCTT
AACCACGTGC AGGAGGTGCT GGAGGGCTAC AAGAAGAAGT ATGAGGAGGA GGTTTCTCTG
AGAGCAACAG CTGAGAACGA GTTTGTGGCT CTGAAGAAGG ATGTGGACTG CGCCTACCTC
CGCAAATCAG ACCTGGAGGC CAATGTGGAG GCCCTGATCC AGGAGATCGA CTTCCTGAGG
CGGCTGTATG AGGAGGAGAT CCGCGTTCTC CAGTCCCACA TCTCAGACAC CTCCGTGGTT
GTCAAGCTGG ACAACAGCCG GGACCTGAAC ATGGACTGCA TCATTGCCGA GATCAAGGCA
CAGTACGATG ACATTGTCAC CCGTAGCCGG GCTGAGGCCG AGTCCTGGTA CCGCAGCAAG
TGTGAGGAGA TGAAGGCCAC GGTGATCAGG CACGGGGAGA CCCTGCGCCG CACCAAGGAG
GAGATCAACG AGCTGAACCG CATGATCCAG AGGCTGACGG CTGAGGTGGA GAATGCCAAG
TGCCAGAATT CCAAGCTGGA GGCTGCGGTG GCTCAGTCTG AGCAGCAGGG TGAGGCGGCC
CTCAGCGATG CCCGCTGCAA GTTGGCCGAG CTGGAGGGTG CCCTGCAGAA GGCCAAGCAG
GACATGGCCT GCCTGATCAG GGAGTACCAG GAGGTGATGA ACTCCAAGCT GGGCCTGGAC
ATCGAGATCG CCACCTACAG GCGCCTGCTG GAGGGCGAGG AGCAGAGGCT GTGCGAGGGC
GTCGGCTCGG TGAATGTCTG CGTCAGCAGC TCCCGCGGTG GCGTTGTCTG TGGCGATCTC
TGCGCCTCCA CTACTGCCCC TGTTGTCTCC ACCAGAGTCA GTAGCGTCCC CAGCAACAGC
AACGTGGTGG TGGGCACTAC TAACGCCTGC GCCCCCTCCG CCCGGGTTGG CGTCTGCGGC
GGCAGCTGTA AGAGGTGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016874785.1
CDS294..1754
Misc Feature(1)<300..599
Misc Feature(2)606..1541
Misc Feature(3)966..1313
Misc Feature(4)<1236..>1442
Translation

Target ORF information:

RefSeq Version XM_017019296.1
Organism Homo sapiens(Human)
Definition Homo sapiens keratin 86 (KRT86), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017019296.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACTTGTG GATCTTACTG TGGTGGCCGC GCCTTCAGCT GCATCTCGGC CTGCGGGCCC 
CGGCCCGGCC GCTGCTGCAT CACCGCCGCC CCCTACCGTG GCATCTCCTG CTACCGCGGC
CTCACCGGGG GCTTCGGCAG CCACAGCGTG TGCGGAGGCT TTCGGGCCGG CTCCTGCGGA
CGCAGCTTCG GCTACCGCTC CGGGGGCGTG TGCGGGCCCA GTCCCCCATG CATCACCACC
GTGTCGGTCA ACGAGAGCCT CCTCACGCCC CTCAACCTGG AGATCGACCC CAACGCGCAG
TGCGTGAAGC AGGAGGAGAA GGAGCAGATC AAGTCCCTCA ACAGCAGGTT CGCGGCCTTC
ATCGACAAGG TGCGCTTCCT GGAGCAGCAG AACAAACTGC TGGAGACAAA GCTGCAGTTC
TACCAGAACC GCGAGTGTTG CCAGAGCAAC CTGGAGCCCC TGTTTGAGGG CTACATCGAG
ACTCTGCGGC GGGAGGCCGA GTGCGTGGAG GCCGACAGCG GGAGGCTGGC CTCAGAGCTT
AACCACGTGC AGGAGGTGCT GGAGGGCTAC AAGAAGAAGT ATGAGGAGGA GGTTTCTCTG
AGAGCAACAG CTGAGAACGA GTTTGTGGCT CTGAAGAAGG ATGTGGACTG CGCCTACCTC
CGCAAATCAG ACCTGGAGGC CAATGTGGAG GCCCTGATCC AGGAGATCGA CTTCCTGAGG
CGGCTGTATG AGGAGGAGAT CCGCGTTCTC CAGTCCCACA TCTCAGACAC CTCCGTGGTT
GTCAAGCTGG ACAACAGCCG GGACCTGAAC ATGGACTGCA TCATTGCCGA GATCAAGGCA
CAGTACGATG ACATTGTCAC CCGTAGCCGG GCTGAGGCCG AGTCCTGGTA CCGCAGCAAG
TGTGAGGAGA TGAAGGCCAC GGTGATCAGG CACGGGGAGA CCCTGCGCCG CACCAAGGAG
GAGATCAACG AGCTGAACCG CATGATCCAG AGGCTGACGG CTGAGGTGGA GAATGCCAAG
TGCCAGAATT CCAAGCTGGA GGCTGCGGTG GCTCAGTCTG AGCAGCAGGG TGAGGCGGCC
CTCAGCGATG CCCGCTGCAA GTTGGCCGAG CTGGAGGGTG CCCTGCAGAA GGCCAAGCAG
GACATGGCCT GCCTGATCAG GGAGTACCAG GAGGTGATGA ACTCCAAGCT GGGCCTGGAC
ATCGAGATCG CCACCTACAG GCGCCTGCTG GAGGGCGAGG AGCAGAGGCT GTGCGAGGGC
GTCGGCTCGG TGAATGTCTG CGTCAGCAGC TCCCGCGGTG GCGTTGTCTG TGGCGATCTC
TGCGCCTCCA CTACTGCCCC TGTTGTCTCC ACCAGAGTCA GTAGCGTCCC CAGCAACAGC
AACGTGGTGG TGGGCACTAC TAACGCCTGC GCCCCCTCCG CCCGGGTTGG CGTCTGCGGC
GGCAGCTGTA AGAGGTGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu46550
Clone ID Related Accession (Same CDS sequence) XM_005268866.4
Accession Version XM_005268866.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1692bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product keratin, type II cuticular Hb6 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268866.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGTGTGAGG AATTAAAGGC AACTGTGCAG AAACACACGC AGAGCCTGAA GCCCAGCAAG 
GAGGATCTGA ACAGGCTTAA TCAGGCCATC CAGTGGCTGA CGGTGGAGGT GGGCAGTGCT
GAGAGTCAGG CCTACAGAGG TGCAAGTAGT GAACGCCTGA CGCCCCGACC ACTGTGCTCT
CCATTCGGAC GTCTCCATCC TCAGAACCTC CTCTCTTCCC CAAAAAGCAC CATGACTTGT
GGATCTTACT GTGGTGGCCG CGCCTTCAGC TGCATCTCGG CCTGCGGGCC CCGGCCCGGC
CGCTGCTGCA TCACCGCCGC CCCCTACCGT GGCATCTCCT GCTACCGCGG CCTCACCGGG
GGCTTCGGCA GCCACAGCGT GTGCGGAGGC TTTCGGGCCG GCTCCTGCGG ACGCAGCTTC
GGCTACCGCT CCGGGGGCGT GTGCGGGCCC AGTCCCCCAT GCATCACCAC CGTGTCGGTC
AACGAGAGCC TCCTCACGCC CCTCAACCTG GAGATCGACC CCAACGCGCA GTGCGTGAAG
CAGGAGGAGA AGGAGCAGAT CAAGTCCCTC AACAGCAGGT TCGCGGCCTT CATCGACAAG
GTGCGCTTCC TGGAGCAGCA GAACAAACTG CTGGAGACAA AGCTGCAGTT CTACCAGAAC
CGCGAGTGTT GCCAGAGCAA CCTGGAGCCC CTGTTTGAGG GCTACATCGA GACTCTGCGG
CGGGAGGCCG AGTGCGTGGA GGCCGACAGC GGGAGGCTGG CCTCAGAGCT TAACCACGTG
CAGGAGGTGC TGGAGGGCTA CAAGAAGAAG TATGAGGAGG AGGTTTCTCT GAGAGCAACA
GCTGAGAACG AGTTTGTGGC TCTGAAGAAG GATGTGGACT GCGCCTACCT CCGCAAATCA
GACCTGGAGG CCAATGTGGA GGCCCTGATC CAGGAGATCG ACTTCCTGAG GCGGCTGTAT
GAGGAGGAGA TCCGCGTTCT CCAGTCCCAC ATCTCAGACA CCTCCGTGGT TGTCAAGCTG
GACAACAGCC GGGACCTGAA CATGGACTGC ATCATTGCCG AGATCAAGGC ACAGTACGAT
GACATTGTCA CCCGTAGCCG GGCTGAGGCC GAGTCCTGGT ACCGCAGCAA GTGTGAGGAG
ATGAAGGCCA CGGTGATCAG GCACGGGGAG ACCCTGCGCC GCACCAAGGA GGAGATCAAC
GAGCTGAACC GCATGATCCA GAGGCTGACG GCTGAGGTGG AGAATGCCAA GTGCCAGAAT
TCCAAGCTGG AGGCTGCGGT GGCTCAGTCT GAGCAGCAGG GTGAGGCGGC CCTCAGCGAT
GCCCGCTGCA AGTTGGCCGA GCTGGAGGGT GCCCTGCAGA AGGCCAAGCA GGACATGGCC
TGCCTGATCA GGGAGTACCA GGAGGTGATG AACTCCAAGC TGGGCCTGGA CATCGAGATC
GCCACCTACA GGCGCCTGCT GGAGGGCGAG GAGCAGAGGC TGTGCGAGGG CGTCGGCTCG
GTGAATGTCT GCGTCAGCAG CTCCCGCGGT GGCGTTGTCT GTGGCGATCT CTGCGCCTCC
ACTACTGCCC CTGTTGTCTC CACCAGAGTC AGTAGCGTCC CCAGCAACAG CAACGTGGTG
GTGGGCACTA CTAACGCCTG CGCCCCCTCC GCCCGGGTTG GCGTCTGCGG CGGCAGCTGT
AAGAGGTGCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005268923.1
CDS4214..5905
Misc Feature(1)<4451..4750
Misc Feature(2)4757..5692
Misc Feature(3)5117..5464
Misc Feature(4)<5387..>5593
Translation

Target ORF information:

RefSeq Version XM_005268866.4
Organism Homo sapiens(Human)
Definition Homo sapiens keratin 86 (KRT86), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005268866.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGTGTGAGG AATTAAAGGC AACTGTGCAG AAACACACGC AGAGCCTGAA GCCCAGCAAG 
GAGGATCTGA ACAGGCTTAA TCAGGCCATC CAGTGGCTGA CGGTGGAGGT GGGCAGTGCT
GAGAGTCAGG CCTACAGAGG TGCAAGTAGT GAACGCCTGA CGCCCCGACC ACTGTGCTCT
CCATTCGGAC GTCTCCATCC TCAGAACCTC CTCTCTTCCC CAAAAAGCAC CATGACTTGT
GGATCTTACT GTGGTGGCCG CGCCTTCAGC TGCATCTCGG CCTGCGGGCC CCGGCCCGGC
CGCTGCTGCA TCACCGCCGC CCCCTACCGT GGCATCTCCT GCTACCGCGG CCTCACCGGG
GGCTTCGGCA GCCACAGCGT GTGCGGAGGC TTTCGGGCCG GCTCCTGCGG ACGCAGCTTC
GGCTACCGCT CCGGGGGCGT GTGCGGGCCC AGTCCCCCAT GCATCACCAC CGTGTCGGTC
AACGAGAGCC TCCTCACGCC CCTCAACCTG GAGATCGACC CCAACGCGCA GTGCGTGAAG
CAGGAGGAGA AGGAGCAGAT CAAGTCCCTC AACAGCAGGT TCGCGGCCTT CATCGACAAG
GTGCGCTTCC TGGAGCAGCA GAACAAACTG CTGGAGACAA AGCTGCAGTT CTACCAGAAC
CGCGAGTGTT GCCAGAGCAA CCTGGAGCCC CTGTTTGAGG GCTACATCGA GACTCTGCGG
CGGGAGGCCG AGTGCGTGGA GGCCGACAGC GGGAGGCTGG CCTCAGAGCT TAACCACGTG
CAGGAGGTGC TGGAGGGCTA CAAGAAGAAG TATGAGGAGG AGGTTTCTCT GAGAGCAACA
GCTGAGAACG AGTTTGTGGC TCTGAAGAAG GATGTGGACT GCGCCTACCT CCGCAAATCA
GACCTGGAGG CCAATGTGGA GGCCCTGATC CAGGAGATCG ACTTCCTGAG GCGGCTGTAT
GAGGAGGAGA TCCGCGTTCT CCAGTCCCAC ATCTCAGACA CCTCCGTGGT TGTCAAGCTG
GACAACAGCC GGGACCTGAA CATGGACTGC ATCATTGCCG AGATCAAGGC ACAGTACGAT
GACATTGTCA CCCGTAGCCG GGCTGAGGCC GAGTCCTGGT ACCGCAGCAA GTGTGAGGAG
ATGAAGGCCA CGGTGATCAG GCACGGGGAG ACCCTGCGCC GCACCAAGGA GGAGATCAAC
GAGCTGAACC GCATGATCCA GAGGCTGACG GCTGAGGTGG AGAATGCCAA GTGCCAGAAT
TCCAAGCTGG AGGCTGCGGT GGCTCAGTCT GAGCAGCAGG GTGAGGCGGC CCTCAGCGAT
GCCCGCTGCA AGTTGGCCGA GCTGGAGGGT GCCCTGCAGA AGGCCAAGCA GGACATGGCC
TGCCTGATCA GGGAGTACCA GGAGGTGATG AACTCCAAGC TGGGCCTGGA CATCGAGATC
GCCACCTACA GGCGCCTGCT GGAGGGCGAG GAGCAGAGGC TGTGCGAGGG CGTCGGCTCG
GTGAATGTCT GCGTCAGCAG CTCCCGCGGT GGCGTTGTCT GTGGCGATCT CTGCGCCTCC
ACTACTGCCC CTGTTGTCTC CACCAGAGTC AGTAGCGTCC CCAGCAACAG CAACGTGGTG
GTGGGCACTA CTAACGCCTG CGCCCCCTCC GCCCGGGTTG GCGTCTGCGG CGGCAGCTGT
AAGAGGTGCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A novel KRT86 mutation in a Turkish family with monilethrix, and identification of maternal mosaicism.
Clinical and experimental dermatology40(7)781-5(2015 Oct)
Redler S,Pasternack SM,Wolf S,Stienen D,Wenzel J,Nöthen MM,Betz RC


book

Novel KRT83 and KRT86 mutations associated with monilethrix.
Experimental dermatology24(3)222-4(2015 Mar)
van Steensel M,Vreeburg M,Urbina MT,López P,Morice-Picard F,van Geel M


book

Mutation detection of type II hair cortex keratin gene KRT86 in a Chinese Han family with congenital monilethrix.
Chinese medical journal126(16)3103-6(2013 Aug)
Ye ZZ,Nan X,Zhao HS,Chen XR,Song QH


book

Congenital monilethrix and hereditary unilateral external auditory canal atresia are co-inherited in a Chinese pedigree with recurrent KRT86 mutation.
The Journal of dermatology39(9)817-9(2012 Sep)
Feng YG,Xiao SX,Xu AL,Feng JY,Wang JM


book

A novel monilethrix mutation in coil 2A of KRT86 causing autosomal dominant monilethrix with incomplete penetrance.
The British journal of dermatology166 Suppl 220-6(2012 Jun)
De Cruz R,Horev L,Green J,Babay S,Sladden M,Zlotogorski A,Sinclair R


 
*
*
*
*