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LEP cDNA ORF clone, Homo sapiens (human)

Gene Symbol LEP
Entrez Gene ID 3952
Full Name leptin
Synonyms LEPD, OB, OBS
General protein information
Preferred Names
leptin
Names
leptin
obese protein
obesity factor
obese, mouse, homolog of
leptin (murine obesity homolog)
leptin (obesity homolog, mouse)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7q31.3

Summary This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

164160

Disorder Html: Obesity, severe, due to leptin deficiency (3); Obesity, morbid, with

mRNA and Protein(s)

mRNA Protein Name
XM_005250340 XP_005250397 leptin isoform X1
NM_000230 NP_000221 leptin precursor



Homo sapiens (human) LEP NP_000221.1
Pan troglodytes (chimpanzee) LEP NP_001180601.1
Macaca mulatta (Rhesus monkey) LEP NP_001036220.1
Canis lupus familiaris (dog) LEP NP_001003070.1
Bos taurus (cattle) LEP NP_776353.2
Mus musculus (house mouse) Lep NP_032519.1
Rattus norvegicus (Norway rat) Lep NP_037208.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100486715 XP_002931881.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LEP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LEP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu41550 XM_005250340 PREDICTED: Homo sapiens leptin (LEP), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu27387
NM_000230 Homo sapiens leptin (LEP), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu41550
Clone ID Related Accession (Same CDS sequence) XM_005250340
Accession Version XM_005250340.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 501bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product leptin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578814244. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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481
ATGCATTGGG GAACCCTGTG CGGATTCTTG TGGCTTTGGC CCTATCTTTT CTATGTCCAA 
GCTGTGCCCA TCCAAAAAGT CCAAGATGAC ACCAAAACCC TCATCAAGAC AATTGTCACC
AGGATCAATG ACATTTCACA CACGTCAGTC TCCTCCAAAC AGAAAGTCAC CGGTTTGGAC
TTCATTCCTG GGCTCCACCC CATCCTGACC TTATCCAAGA TGGACCAGAC ACTGGCAGTC
TACCAACAGA TCCTCACCAG TATGCCTTCC AGAAACGTGA TCCAAATATC CAACGACCTG
GAGAACCTCC GGGATCTTCT TCACGTGCTG GCCTTCTCTA AGAGCTGCCA CTTGCCCTGG
GCCAGTGGCC TGGAGACCTT GGACAGCCTG GGGGGTGTCC TGGAAGCTTC AGGCTACTCC
ACAGAGGTGG TGGCCCTGAG CAGGCTGCAG GGGTCTCTGC AGGACATGCT GTGGCAGCTG
GACCTCAGCC CTGGGTGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005250397.1
CDS134..634
Misc Feature(1)197..631(+)
Translation

Target ORF information:

RefSeq Version XM_005250340
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens leptin (LEP), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005250340

ORF Insert Sequence:

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61
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181
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361
421
481
ATGCATTGGG GAACCCTGTG CGGATTCTTG TGGCTTTGGC CCTATCTTTT CTATGTCCAA 
GCTGTGCCCA TCCAAAAAGT CCAAGATGAC ACCAAAACCC TCATCAAGAC AATTGTCACC
AGGATCAATG ACATTTCACA CACGTCAGTC TCCTCCAAAC AGAAAGTCAC CGGTTTGGAC
TTCATTCCTG GGCTCCACCC CATCCTGACC TTATCCAAGA TGGACCAGAC ACTGGCAGTC
TACCAACAGA TCCTCACCAG TATGCCTTCC AGAAACGTGA TCCAAATATC CAACGACCTG
GAGAACCTCC GGGATCTTCT TCACGTGCTG GCCTTCTCTA AGAGCTGCCA CTTGCCCTGG
GCCAGTGGCC TGGAGACCTT GGACAGCCTG GGGGGTGTCC TGGAAGCTTC AGGCTACTCC
ACAGAGGTGG TGGCCCTGAG CAGGCTGCAG GGGTCTCTGC AGGACATGCT GTGGCAGCTG
GACCTCAGCC CTGGGTGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27387
Clone ID Related Accession (Same CDS sequence) NM_000230
Accession Version NM_000230.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 504bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product leptin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA762132.1, U43653.1, AC018635.10 and BU752306.1. This sequence is a reference standard in the RefSeqGene project. On Mar 12, 2008 this sequence version replaced gi:4557714. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U43653.1, BC060830.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

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ATGCATTGGG GAACCCTGTG CGGATTCTTG TGGCTTTGGC CCTATCTTTT CTATGTCCAA 
GCTGTGCCCA TCCAAAAAGT CCAAGATGAC ACCAAAACCC TCATCAAGAC AATTGTCACC
AGGATCAATG ACATTTCACA CACGCAGTCA GTCTCCTCCA AACAGAAAGT CACCGGTTTG
GACTTCATTC CTGGGCTCCA CCCCATCCTG ACCTTATCCA AGATGGACCA GACACTGGCA
GTCTACCAAC AGATCCTCAC CAGTATGCCT TCCAGAAACG TGATCCAAAT ATCCAACGAC
CTGGAGAACC TCCGGGATCT TCTTCACGTG CTGGCCTTCT CTAAGAGCTG CCACTTGCCC
TGGGCCAGTG GCCTGGAGAC CTTGGACAGC CTGGGGGGTG TCCTGGAAGC TTCAGGCTAC
TCCACAGAGG TGGTGGCCCT GAGCAGGCTG CAGGGGTCTC TGCAGGACAT GCTGTGGCAG
CTGGACCTCA GCCCTGGGTG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000221.1
CDS58..561
Misc Feature(1)121..558(+)
Exon (1)1..29
Gene:LEP
Gene Synonym:
Exon (2)30..201
Gene:LEP
Gene Synonym:
Exon (3)202..3427
Gene:LEP
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000230
Organism Homo sapiens (human)
Definition Homo sapiens leptin (LEP), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000230

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCATTGGG GAACCCTGTG CGGATTCTTG TGGCTTTGGC CCTATCTTTT CTATGTCCAA 
GCTGTGCCCA TCCAAAAAGT CCAAGATGAC ACCAAAACCC TCATCAAGAC AATTGTCACC
AGGATCAATG ACATTTCACA CACGCAGTCA GTCTCCTCCA AACAGAAAGT CACCGGTTTG
GACTTCATTC CTGGGCTCCA CCCCATCCTG ACCTTATCCA AGATGGACCA GACACTGGCA
GTCTACCAAC AGATCCTCAC CAGTATGCCT TCCAGAAACG TGATCCAAAT ATCCAACGAC
CTGGAGAACC TCCGGGATCT TCTTCACGTG CTGGCCTTCT CTAAGAGCTG CCACTTGCCC
TGGGCCAGTG GCCTGGAGAC CTTGGACAGC CTGGGGGGTG TCCTGGAAGC TTCAGGCTAC
TCCACAGAGG TGGTGGCCCT GAGCAGGCTG CAGGGGTCTC TGCAGGACAT GCTGTGGCAG
CTGGACCTCA GCCCTGGGTG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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