MSH3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MSH3
Entrez Gene ID	4437
Full Name	mutS homolog 3
Synonyms	DUP,FAP4,MRP1
General protein information	Preferred Names mutS homolog 3 Names DNA mismatch repair protein Msh3 divergent upstream protein hMSH3 mismatch repair protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q14.1
Summary	The protein encoded by this gene forms a heterodimer with MSH2 to form MutS beta, part of the post-replicative DNA mismatch repair system. MutS beta initiates mismatch repair by binding to a mismatch and then forming a complex with MutL alpha heterodimer. This gene contains a polymorphic 9 bp tandem repeat sequence in the first exon. The repeat is present 6 times in the reference genome sequence and 3-7 repeats have been reported. Defects in this gene are a cause of susceptibility to endometrial cancer. [provided by RefSeq, Mar 2011].
Disorder MIM:	600887
Disorder Html:	Endometrial carcinoma, somatic, 608089 (3); Familial adenomatous polyposis 4, 617100 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002439.5	NP_002430.3	DNA mismatch repair protein Msh3
NM_002439.4	NP_002430.3	DNA mismatch repair protein Msh3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MSH3	NP_001095775.1
Oryza sativa (rice)	Os04g0682900	NP_001054300.1
Schizosaccharomyces pombe (fission yeast)	msh3	NP_593952.1
Gallus gallus (chicken)	MSH3	XP_004949519.1
Danio rerio (zebrafish)	LOC100537314	XP_003198705.2
Homo sapiens (human)	MSH3	NP_002430.3
Pan troglodytes (chimpanzee)	MSH3	XP_001134751.1
	MSH3	XP_001110439.1
Arabidopsis thaliana (thale cress)	MSH3	NP_194284.2
Xenopus tropicalis (tropical clawed frog)	msh3	XP_002942407.2
Canis lupus familiaris (dog)	MSH3	XP_005618220.1
Mus musculus (house mouse)	Msh3	NP_034959.2
Rattus norvegicus (Norway rat)	Msh3	NP_001178886.1
Danio rerio (zebrafish)	msh3	NP_001103184.1
Magnaporthe oryzae (rice blast fungus)	MGG_09306	XP_003709956.1
Neurospora crassa	NCU08115	XP_963874.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of MSH2 and MSH6 due to heterozygous germline defects in MSH3 and MSH6.
Morak M, K?sbauer S, Kerscher M, Laner A, Nissen AM, Benet-Pag?s A, Schackert HK, Keller G, Massdorf T, Holinski-Feder E
Familial cancer16(4)491-500(2017 Oct)

Identification of genetic variants associated with Huntington's disease progression: a genome-wide association study.
Hensman Moss DJ, Pardi?as AF, Langbehn D, Lo K, Leavitt BR, Roos R, Durr A, Mead S, , , Holmans P, Jones L, Tabrizi SJ
The Lancet. Neurology16(9)701-711(2017 09)

Inactivation of MSH3 by promoter methylation correlates with primary tumor stage in nasopharyngeal carcinoma.
Ni H, Jiang B, Zhou Z, Yuan X, Cao X, Huang G, Li Y
International journal of molecular medicine40(3)673-678(2017 Sep)

MutS? abundance and Msh3 ATP hydrolysis activity are important drivers of CTG?CAG repeat expansions.
Keogh N, Chan KY, Li GM, Lahue RS
Nucleic acids research45(17)10068-10078(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MSH3 is probably a modifier of disease progression in Huntington's disease.
Title: Identification of genetic variants associated with Huntington's disease progression: a genome-wide association study.

Msh3-/- cells are severely defective for CTG*CAG repeat expansions but show full activity on contractions. Msh3 overexpression led to high expansion activity and elevated levels of MutSbeta complex, indicating that MutSbeta abundance drives expansions. Expression of 2 Msh3 polymorphic variants at normal levels showed no detectable change in expansions. These polymorphisms primarily affect Msh3 protein stability, not ac...
Title: MutS? abundance and Msh3 ATP hydrolysis activity are important drivers of CTG?CAG repeat expansions.

Findings indicate that carriers of the MSH5 rs707939 T allele, the MSH2 rs6544991 C allele, the MSH3 rs6151627 and rs6151670 G alleles, and the MSH3 rs7709909 T allele have poor toxicity tolerance to platinum-based chemotherapy in non-small cell lung cancer patients.
Title: Association between DNA mismatch repair gene polymorphisms and platinum-based chemotherapy toxicity in non-small cell lung cancer patients.

data suggest that MSH3 mutations represent an additional recessive subtype of colorectal adenomatous polyposis
Title: Exome Sequencing Identifies Biallelic MSH3 Germline Mutations as a Recessive Subtype of Colorectal Adenomatous Polyposis.

Three polymorphisms in MSH3 were associated with variation in somatic instability in myotonic dystrophy type 1.
Title: A polymorphism in the MSH3 mismatch repair gene is associated with the levels of somatic instability of the expanded CTG repeat in the blood DNA of myotonic dystrophy type 1 patients.

The following MSH3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MSH3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20501	NM_002439.5 Latest version!	Homo sapiens mutS homolog 3 (MSH3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
View Old Accession Versions >>
OHu20501	NM_002439.4	Homo sapiens mutS homolog 3 (MSH3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MSH3 ( NM_002439.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002430.3 Homo sapiens mutS homolog 3 (MSH3), mRNA.

Price & Availability↑

CloneID	OHu20501
Clone ID Related Accession (Same CDS sequence)	NM_002439.5 , NM_002439.4
Accession Version	NM_002439.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	DNA mismatch repair protein Msh3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC130434.1, J04810.1, AC022493.12, U61981.1 and AA601983.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002439.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U61981.1, SRR1803615.35610.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265081.7/ ENSP00000265081.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTCGCC GGAAGCCTGC GTCGGGCGGC CTCGCTGCCT CCAGCTCAGC CCCTGCGAGG 
CAAGCGGTTT TGAGCCGATT CTTCCAGTCT ACGGGAAGCC TGAAATCCAC CTCCTCCTCC 
ACAGGTGCAG CCGACCAGGT GGACCCTGGC GCTGCAGCGG CTGCAGCGGC CGCAGCGGCC 
GCAGCGCCCC CAGCGCCCCC AGCTCCCGCC TTCCCGCCCC AGCTGCCGCC GCACATAGCT 
ACAGAAATTG ACAGAAGAAA GAAGAGACCA TTGGAAAATG ATGGGCCTGT TAAAAAGAAA 
GTAAAGAAAG TCCAACAAAA GGAAGGAGGA AGTGATCTGG GAATGTCTGG CAACTCTGAG 
CCAAAGAAAT GTCTGAGGAC CAGGAATGTT TCAAAGTCTC TGGAAAAATT GAAAGAATTC 
TGCTGCGATT CTGCCCTTCC TCAAAGTAGA GTCCAGACAG AATCTCTGCA GGAGAGATTT 
GCAGTTCTGC CAAAATGTAC TGATTTTGAT GATATCAGTC TTCTACACGC AAAGAATGCA 
GTTTCTTCTG AAGATTCGAA ACGTCAAATT AATCAAAAGG ACACAACACT TTTTGATCTC 
AGTCAGTTTG GATCATCAAA TACAAGTCAT GAAAATTTAC AGAAAACTGC TTCCAAATCA 
GCTAACAAAC GGTCCAAAAG CATCTATACG CCGCTAGAAT TACAATACAT AGAAATGAAG 
CAGCAGCACA AAGATGCAGT TTTGTGTGTG GAATGTGGAT ATAAGTATAG ATTCTTTGGG 
GAAGATGCAG AGATTGCAGC CCGAGAGCTC AATATTTATT GCCATTTAGA TCACAACTTT 
ATGACAGCAA GTATACCTAC TCACAGACTG TTTGTTCATG TACGCCGCCT GGTGGCAAAA 
GGATATAAGG TGGGAGTTGT GAAGCAAACT GAAACTGCAG CATTAAAGGC CATTGGAGAC 
AACAGAAGTT CACTCTTTTC CCGGAAATTG ACTGCCCTTT ATACAAAATC TACACTTATT 
GGAGAAGATG TGAATCCCCT AATCAAGCTG GATGATGCTG TAAATGTTGA TGAGATAATG 
ACTGATACTT CTACCAGCTA TCTTCTGTGC ATCTCTGAAA ATAAGGAAAA TGTTAGGGAC 
AAAAAAAAGG GCAACATTTT TATTGGCATT GTGGGAGTGC AGCCTGCCAC AGGCGAGGTT 
GTGTTTGATA GTTTCCAGGA CTCTGCTTCT CGTTCAGAGC TAGAAACCCG GATGTCAAGC 
CTGCAGCCAG TAGAGCTGCT GCTTCCTTCG GCCTTGTCCG AGCAAACAGA GGCGCTCATC 
CACAGAGCCA CATCTGTTAG TGTGCAGGAT GACAGAATTC GAGTCGAAAG GATGGATAAC 
ATTTATTTTG AATACAGCCA TGCTTTCCAG GCAGTTACAG AGTTTTATGC AAAAGATACA 
GTTGACATCA AAGGTTCTCA AATTATTTCT GGCATTGTTA ACTTAGAGAA GCCTGTGATT 
TGCTCTTTGG CTGCCATCAT AAAATACCTC AAAGAATTCA ACTTGGAAAA GATGCTCTCC 
AAACCTGAGA ATTTTAAACA GCTATCAAGT AAAATGGAAT TTATGACAAT TAATGGAACA 
ACATTAAGGA ATCTGGAAAT CCTACAGAAT CAGACTGATA TGAAAACCAA AGGAAGTTTG 
CTGTGGGTTT TAGACCACAC TAAAACTTCA TTTGGGAGAC GGAAGTTAAA GAAGTGGGTG 
ACCCAGCCAC TCCTTAAATT AAGGGAAATA AATGCCCGGC TTGATGCTGT ATCGGAAGTT 
CTCCATTCAG AATCTAGTGT GTTTGGTCAG ATAGAAAATC ATCTACGTAA ATTGCCCGAC 
ATAGAGAGGG GACTCTGTAG CATTTATCAC AAAAAATGTT CTACCCAAGA GTTCTTCTTG 
ATTGTCAAAA CTTTATATCA CCTAAAGTCA GAATTTCAAG CAATAATACC TGCTGTTAAT 
TCCCACATTC AGTCAGACTT GCTCCGGACC GTTATTTTAG AAATTCCTGA ACTCCTCAGT 
CCAGTGGAGC ATTACTTAAA GATACTCAAT GAACAAGCTG CCAAAGTTGG GGATAAAACT 
GAATTATTTA AAGACCTTTC TGACTTCCCT TTAATAAAAA AGAGGAAGGA TGAAATTCAA 
GGTGTTATTG ACGAGATCCG AATGCATTTG CAAGAAATAC GAAAAATACT AAAAAATCCT 
TCTGCACAAT ATGTGACAGT ATCAGGACAG GAGTTTATGA TAGAAATAAA GAACTCTGCT 
GTATCTTGTA TACCAACTGA TTGGGTAAAG GTTGGAAGCA CAAAAGCTGT GAGCCGCTTT 
CACTCTCCTT TTATTGTAGA AAATTACAGA CATCTGAATC AGCTCCGGGA GCAGCTAGTC 
CTTGACTGCA GTGCTGAATG GCTTGATTTT CTAGAGAAAT TCAGTGAACA TTATCACTCC 
TTGTGTAAAG CAGTGCATCA CCTAGCAACT GTTGACTGCA TTTTCTCCCT GGCCAAGGTC 
GCTAAGCAAG GAGATTACTG CAGACCAACT GTACAAGAAG AAAGAAAAAT TGTAATAAAA 
AATGGAAGGC ACCCTGTGAT TGATGTGTTG CTGGGAGAAC AGGATCAATA TGTCCCAAAT 
AATACAGATT TATCAGAGGA CTCAGAGAGA GTAATGATAA TTACCGGACC AAACATGGGT 
GGAAAGAGCT CCTACATAAA ACAAGTTGCA TTGATTACCA TCATGGCTCA GATTGGCTCC 
TATGTTCCTG CAGAAGAAGC GACAATTGGG ATTGTGGATG GCATTTTCAC AAGGATGGGT 
GCTGCAGACA ATATATATAA AGGACAGAGT ACATTTATGG AAGAACTGAC TGACACAGCA 
GAAATAATCA GAAAAGCAAC ATCACAGTCC TTGGTTATCT TGGATGAACT AGGAAGAGGG 
ACGAGCACTC ATGATGGAAT TGCCATTGCC TATGCTACAC TTGAGTATTT CATCAGAGAT 
GTGAAATCCT TAACCCTGTT TGTCACCCAT TATCCGCCAG TTTGTGAACT AGAAAAAAAT 
TACTCACACC AGGTGGGGAA TTACCACATG GGATTCTTGG TCAGTGAGGA TGAAAGCAAA 
CTGGATCCAG GCGCAGCAGA ACAAGTCCCT GATTTTGTCA CCTTCCTTTA CCAAATAACT 
AGAGGAATTG CAGCAAGGAG TTATGGATTA AATGTGGCTA AACTAGCAGA TGTTCCTGGA 
GAAATTTTGA AGAAAGCAGC TCACAAGTCA AAAGAGCTGG AAGGATTAAT AAATACGAAA 
AGAAAGAGAC TCAAGTATTT TGCAAAGTTA TGGACGATGC ATAATGCACA AGACCTGCAG 
AAGTGGACAG AGGAGTTCAA CATGGAAGAA ACACAGACTT CTCTTCTTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002430.3
CDS	77..3490
Translation	MSRRKPASGGLAASSSAPARQAVLSRFFQSTGSLKSTSSSTGAADQVDPGAAAAAAAAAAAAPPAPPAPAFPPQLPPHIATEIDRRKKRPLENDGPVKKKVKKVQQKEGGSDLGMSGNSEPKKCLRTRNVSKSLEKLKEFCCDSALPQSRVQTESLQERFAVLPKCTDFDDISLLHAKNAVSSEDSKRQINQKDTTLFDLSQFGSSNTSHENLQKTASKSANKRSKSIYTPLELQYIEMKQQHKDAVLCVECGYKYRFFGEDAEIAARELNIYCHLDHNFMTASIPTHRLFVHVRRLVAKGYKVGVVKQTETAALKAIGDNRSSLFSRKLTALYTKSTLIGEDVNPLIKLDDAVNVDEIMTDTSTSYLLCISENKENVRDKKKGNIFIGIVGVQPATGEVVFDSFQDSASRSELETRMSSLQPVELLLPSALSEQTEALIHRATSVSVQDDRIRVERMDNIYFEYSHAFQAVTEFYAKDTVDIKGSQIISGIVNLEKPVICSLAAIIKYLKEFNLEKMLSKPENFKQLSSKMEFMTINGTTLRNLEILQNQTDMKTKGSLLWVLDHTKTSFGRRKLKKWVTQPLLKLREINARLDAVSEVLHSESSVFGQIENHLRKLPDIERGLCSIYHKKCSTQEFFLIVKTLYHLKSEFQAIIPAVNSHIQSDLLRTVILEIPELLSPVEHYLKILNEQAAKVGDKTELFKDLSDFPLIKKRKDEIQGVIDEIRMHLQEIRKILKNPSAQYVTVSGQEFMIEIKNSAVSCIPTDWVKVGSTKAVSRFHSPFIVENYRHLNQLREQLVLDCSAEWLDFLEKFSEHYHSLCKAVHHLATVDCIFSLAKVAKQGDYCRPTVQEERKIVIKNGRHPVIDVLLGEQDQYVPNNTDLSEDSERVMIITGPNMGGKSSYIKQVALITIMAQIGSYVPAEEATIGIVDGIFTRMGAADNIYKGQSTFMEELTDTAEIIRKATSQSLVILDELGRGTSTHDGIAIAYATLEYFIRDVKSLTLFVTHYPPVCELEKNYSHQVGNYHMGFLVSEDESKLDPGAAEQVPDFVTFLYQITRGIAARSYGLNVAKLADVPGEILKKAAHKSKELEGLINTKRKRLKYFAKLWTMHNAQDLQKWTEEFNMEETQTSLLH

Target ORF information:

RefSeq Version	NM_002439.5
Organism	Homo sapiens(human)
Definition	Homo sapiens mutS homolog 3 (MSH3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002439.5

ORF Insert Sequence:

ATGTCTCGCC GGAAGCCTGC GTCGGGCGGC CTCGCTGCCT CCAGCTCAGC CCCTGCGAGG 
CAAGCGGTTT TGAGCCGATT CTTCCAGTCT ACGGGAAGCC TGAAATCCAC CTCCTCCTCC 
ACAGGTGCAG CCGACCAGGT GGACCCTGGC GCTGCAGCGG CTGCAGCGGC CGCAGCGGCC 
GCAGCGCCCC CAGCGCCCCC AGCTCCCGCC TTCCCGCCCC AGCTGCCGCC GCACATAGCT 
ACAGAAATTG ACAGAAGAAA GAAGAGACCA TTGGAAAATG ATGGGCCTGT TAAAAAGAAA 
GTAAAGAAAG TCCAACAAAA GGAAGGAGGA AGTGATCTGG GAATGTCTGG CAACTCTGAG 
CCAAAGAAAT GTCTGAGGAC CAGGAATGTT TCAAAGTCTC TGGAAAAATT GAAAGAATTC 
TGCTGCGATT CTGCCCTTCC TCAAAGTAGA GTCCAGACAG AATCTCTGCA GGAGAGATTT 
GCAGTTCTGC CAAAATGTAC TGATTTTGAT GATATCAGTC TTCTACACGC AAAGAATGCA 
GTTTCTTCTG AAGATTCGAA ACGTCAAATT AATCAAAAGG ACACAACACT TTTTGATCTC 
AGTCAGTTTG GATCATCAAA TACAAGTCAT GAAAATTTAC AGAAAACTGC TTCCAAATCA 
GCTAACAAAC GGTCCAAAAG CATCTATACG CCGCTAGAAT TACAATACAT AGAAATGAAG 
CAGCAGCACA AAGATGCAGT TTTGTGTGTG GAATGTGGAT ATAAGTATAG ATTCTTTGGG 
GAAGATGCAG AGATTGCAGC CCGAGAGCTC AATATTTATT GCCATTTAGA TCACAACTTT 
ATGACAGCAA GTATACCTAC TCACAGACTG TTTGTTCATG TACGCCGCCT GGTGGCAAAA 
GGATATAAGG TGGGAGTTGT GAAGCAAACT GAAACTGCAG CATTAAAGGC CATTGGAGAC 
AACAGAAGTT CACTCTTTTC CCGGAAATTG ACTGCCCTTT ATACAAAATC TACACTTATT 
GGAGAAGATG TGAATCCCCT AATCAAGCTG GATGATGCTG TAAATGTTGA TGAGATAATG 
ACTGATACTT CTACCAGCTA TCTTCTGTGC ATCTCTGAAA ATAAGGAAAA TGTTAGGGAC 
AAAAAAAAGG GCAACATTTT TATTGGCATT GTGGGAGTGC AGCCTGCCAC AGGCGAGGTT 
GTGTTTGATA GTTTCCAGGA CTCTGCTTCT CGTTCAGAGC TAGAAACCCG GATGTCAAGC 
CTGCAGCCAG TAGAGCTGCT GCTTCCTTCG GCCTTGTCCG AGCAAACAGA GGCGCTCATC 
CACAGAGCCA CATCTGTTAG TGTGCAGGAT GACAGAATTC GAGTCGAAAG GATGGATAAC 
ATTTATTTTG AATACAGCCA TGCTTTCCAG GCAGTTACAG AGTTTTATGC AAAAGATACA 
GTTGACATCA AAGGTTCTCA AATTATTTCT GGCATTGTTA ACTTAGAGAA GCCTGTGATT 
TGCTCTTTGG CTGCCATCAT AAAATACCTC AAAGAATTCA ACTTGGAAAA GATGCTCTCC 
AAACCTGAGA ATTTTAAACA GCTATCAAGT AAAATGGAAT TTATGACAAT TAATGGAACA 
ACATTAAGGA ATCTGGAAAT CCTACAGAAT CAGACTGATA TGAAAACCAA AGGAAGTTTG 
CTGTGGGTTT TAGACCACAC TAAAACTTCA TTTGGGAGAC GGAAGTTAAA GAAGTGGGTG 
ACCCAGCCAC TCCTTAAATT AAGGGAAATA AATGCCCGGC TTGATGCTGT ATCGGAAGTT 
CTCCATTCAG AATCTAGTGT GTTTGGTCAG ATAGAAAATC ATCTACGTAA ATTGCCCGAC 
ATAGAGAGGG GACTCTGTAG CATTTATCAC AAAAAATGTT CTACCCAAGA GTTCTTCTTG 
ATTGTCAAAA CTTTATATCA CCTAAAGTCA GAATTTCAAG CAATAATACC TGCTGTTAAT 
TCCCACATTC AGTCAGACTT GCTCCGGACC GTTATTTTAG AAATTCCTGA ACTCCTCAGT 
CCAGTGGAGC ATTACTTAAA GATACTCAAT GAACAAGCTG CCAAAGTTGG GGATAAAACT 
GAATTATTTA AAGACCTTTC TGACTTCCCT TTAATAAAAA AGAGGAAGGA TGAAATTCAA 
GGTGTTATTG ACGAGATCCG AATGCATTTG CAAGAAATAC GAAAAATACT AAAAAATCCT 
TCTGCACAAT ATGTGACAGT ATCAGGACAG GAGTTTATGA TAGAAATAAA GAACTCTGCT 
GTATCTTGTA TACCAACTGA TTGGGTAAAG GTTGGAAGCA CAAAAGCTGT GAGCCGCTTT 
CACTCTCCTT TTATTGTAGA AAATTACAGA CATCTGAATC AGCTCCGGGA GCAGCTAGTC 
CTTGACTGCA GTGCTGAATG GCTTGATTTT CTAGAGAAAT TCAGTGAACA TTATCACTCC 
TTGTGTAAAG CAGTGCATCA CCTAGCAACT GTTGACTGCA TTTTCTCCCT GGCCAAGGTC 
GCTAAGCAAG GAGATTACTG CAGACCAACT GTACAAGAAG AAAGAAAAAT TGTAATAAAA 
AATGGAAGGC ACCCTGTGAT TGATGTGTTG CTGGGAGAAC AGGATCAATA TGTCCCAAAT 
AATACAGATT TATCAGAGGA CTCAGAGAGA GTAATGATAA TTACCGGACC AAACATGGGT 
GGAAAGAGCT CCTACATAAA ACAAGTTGCA TTGATTACCA TCATGGCTCA GATTGGCTCC 
TATGTTCCTG CAGAAGAAGC GACAATTGGG ATTGTGGATG GCATTTTCAC AAGGATGGGT 
GCTGCAGACA ATATATATAA AGGACAGAGT ACATTTATGG AAGAACTGAC TGACACAGCA 
GAAATAATCA GAAAAGCAAC ATCACAGTCC TTGGTTATCT TGGATGAACT AGGAAGAGGG 
ACGAGCACTC ATGATGGAAT TGCCATTGCC TATGCTACAC TTGAGTATTT CATCAGAGAT 
GTGAAATCCT TAACCCTGTT TGTCACCCAT TATCCGCCAG TTTGTGAACT AGAAAAAAAT 
TACTCACACC AGGTGGGGAA TTACCACATG GGATTCTTGG TCAGTGAGGA TGAAAGCAAA 
CTGGATCCAG GCGCAGCAGA ACAAGTCCCT GATTTTGTCA CCTTCCTTTA CCAAATAACT 
AGAGGAATTG CAGCAAGGAG TTATGGATTA AATGTGGCTA AACTAGCAGA TGTTCCTGGA 
GAAATTTTGA AGAAAGCAGC TCACAAGTCA AAAGAGCTGG AAGGATTAAT AAATACGAAA 
AGAAAGAGAC TCAAGTATTT TGCAAAGTTA TGGACGATGC ATAATGCACA AGACCTGCAG 
AAGTGGACAG AGGAGTTCAA CATGGAAGAA ACACAGACTT CTCTTCTTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MSH3 ( NM_002439.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002430.3 Homo sapiens mutS homolog 3 (MSH3), mRNA.

Price & Availability↑

CloneID	OHu20501
Clone ID Related Accession (Same CDS sequence)	NM_002439.5 , NM_002439.4
Accession Version	NM_002439.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	DNA mismatch repair protein Msh3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC130434.1, J04810.1, AC022493.12, U61981.1 and AA601983.1. This sequence is a reference standard in the RefSeqGene project. On Jun 9, 2012 this sequence version replaced NM_002439.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U61981.1, SRR1803615.35610.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCGCC GGAAGCCTGC GTCGGGCGGC CTCGCTGCCT CCAGCTCAGC CCCTGCGAGG 
CAAGCGGTTT TGAGCCGATT CTTCCAGTCT ACGGGAAGCC TGAAATCCAC CTCCTCCTCC 
ACAGGTGCAG CCGACCAGGT GGACCCTGGC GCTGCAGCGG CTGCAGCGGC CGCAGCGGCC 
GCAGCGCCCC CAGCGCCCCC AGCTCCCGCC TTCCCGCCCC AGCTGCCGCC GCACATAGCT 
ACAGAAATTG ACAGAAGAAA GAAGAGACCA TTGGAAAATG ATGGGCCTGT TAAAAAGAAA 
GTAAAGAAAG TCCAACAAAA GGAAGGAGGA AGTGATCTGG GAATGTCTGG CAACTCTGAG 
CCAAAGAAAT GTCTGAGGAC CAGGAATGTT TCAAAGTCTC TGGAAAAATT GAAAGAATTC 
TGCTGCGATT CTGCCCTTCC TCAAAGTAGA GTCCAGACAG AATCTCTGCA GGAGAGATTT 
GCAGTTCTGC CAAAATGTAC TGATTTTGAT GATATCAGTC TTCTACACGC AAAGAATGCA 
GTTTCTTCTG AAGATTCGAA ACGTCAAATT AATCAAAAGG ACACAACACT TTTTGATCTC 
AGTCAGTTTG GATCATCAAA TACAAGTCAT GAAAATTTAC AGAAAACTGC TTCCAAATCA 
GCTAACAAAC GGTCCAAAAG CATCTATACG CCGCTAGAAT TACAATACAT AGAAATGAAG 
CAGCAGCACA AAGATGCAGT TTTGTGTGTG GAATGTGGAT ATAAGTATAG ATTCTTTGGG 
GAAGATGCAG AGATTGCAGC CCGAGAGCTC AATATTTATT GCCATTTAGA TCACAACTTT 
ATGACAGCAA GTATACCTAC TCACAGACTG TTTGTTCATG TACGCCGCCT GGTGGCAAAA 
GGATATAAGG TGGGAGTTGT GAAGCAAACT GAAACTGCAG CATTAAAGGC CATTGGAGAC 
AACAGAAGTT CACTCTTTTC CCGGAAATTG ACTGCCCTTT ATACAAAATC TACACTTATT 
GGAGAAGATG TGAATCCCCT AATCAAGCTG GATGATGCTG TAAATGTTGA TGAGATAATG 
ACTGATACTT CTACCAGCTA TCTTCTGTGC ATCTCTGAAA ATAAGGAAAA TGTTAGGGAC 
AAAAAAAAGG GCAACATTTT TATTGGCATT GTGGGAGTGC AGCCTGCCAC AGGCGAGGTT 
GTGTTTGATA GTTTCCAGGA CTCTGCTTCT CGTTCAGAGC TAGAAACCCG GATGTCAAGC 
CTGCAGCCAG TAGAGCTGCT GCTTCCTTCG GCCTTGTCCG AGCAAACAGA GGCGCTCATC 
CACAGAGCCA CATCTGTTAG TGTGCAGGAT GACAGAATTC GAGTCGAAAG GATGGATAAC 
ATTTATTTTG AATACAGCCA TGCTTTCCAG GCAGTTACAG AGTTTTATGC AAAAGATACA 
GTTGACATCA AAGGTTCTCA AATTATTTCT GGCATTGTTA ACTTAGAGAA GCCTGTGATT 
TGCTCTTTGG CTGCCATCAT AAAATACCTC AAAGAATTCA ACTTGGAAAA GATGCTCTCC 
AAACCTGAGA ATTTTAAACA GCTATCAAGT AAAATGGAAT TTATGACAAT TAATGGAACA 
ACATTAAGGA ATCTGGAAAT CCTACAGAAT CAGACTGATA TGAAAACCAA AGGAAGTTTG 
CTGTGGGTTT TAGACCACAC TAAAACTTCA TTTGGGAGAC GGAAGTTAAA GAAGTGGGTG 
ACCCAGCCAC TCCTTAAATT AAGGGAAATA AATGCCCGGC TTGATGCTGT ATCGGAAGTT 
CTCCATTCAG AATCTAGTGT GTTTGGTCAG ATAGAAAATC ATCTACGTAA ATTGCCCGAC 
ATAGAGAGGG GACTCTGTAG CATTTATCAC AAAAAATGTT CTACCCAAGA GTTCTTCTTG 
ATTGTCAAAA CTTTATATCA CCTAAAGTCA GAATTTCAAG CAATAATACC TGCTGTTAAT 
TCCCACATTC AGTCAGACTT GCTCCGGACC GTTATTTTAG AAATTCCTGA ACTCCTCAGT 
CCAGTGGAGC ATTACTTAAA GATACTCAAT GAACAAGCTG CCAAAGTTGG GGATAAAACT 
GAATTATTTA AAGACCTTTC TGACTTCCCT TTAATAAAAA AGAGGAAGGA TGAAATTCAA 
GGTGTTATTG ACGAGATCCG AATGCATTTG CAAGAAATAC GAAAAATACT AAAAAATCCT 
TCTGCACAAT ATGTGACAGT ATCAGGACAG GAGTTTATGA TAGAAATAAA GAACTCTGCT 
GTATCTTGTA TACCAACTGA TTGGGTAAAG GTTGGAAGCA CAAAAGCTGT GAGCCGCTTT 
CACTCTCCTT TTATTGTAGA AAATTACAGA CATCTGAATC AGCTCCGGGA GCAGCTAGTC 
CTTGACTGCA GTGCTGAATG GCTTGATTTT CTAGAGAAAT TCAGTGAACA TTATCACTCC 
TTGTGTAAAG CAGTGCATCA CCTAGCAACT GTTGACTGCA TTTTCTCCCT GGCCAAGGTC 
GCTAAGCAAG GAGATTACTG CAGACCAACT GTACAAGAAG AAAGAAAAAT TGTAATAAAA 
AATGGAAGGC ACCCTGTGAT TGATGTGTTG CTGGGAGAAC AGGATCAATA TGTCCCAAAT 
AATACAGATT TATCAGAGGA CTCAGAGAGA GTAATGATAA TTACCGGACC AAACATGGGT 
GGAAAGAGCT CCTACATAAA ACAAGTTGCA TTGATTACCA TCATGGCTCA GATTGGCTCC 
TATGTTCCTG CAGAAGAAGC GACAATTGGG ATTGTGGATG GCATTTTCAC AAGGATGGGT 
GCTGCAGACA ATATATATAA AGGACAGAGT ACATTTATGG AAGAACTGAC TGACACAGCA 
GAAATAATCA GAAAAGCAAC ATCACAGTCC TTGGTTATCT TGGATGAACT AGGAAGAGGG 
ACGAGCACTC ATGATGGAAT TGCCATTGCC TATGCTACAC TTGAGTATTT CATCAGAGAT 
GTGAAATCCT TAACCCTGTT TGTCACCCAT TATCCGCCAG TTTGTGAACT AGAAAAAAAT 
TACTCACACC AGGTGGGGAA TTACCACATG GGATTCTTGG TCAGTGAGGA TGAAAGCAAA 
CTGGATCCAG GCGCAGCAGA ACAAGTCCCT GATTTTGTCA CCTTCCTTTA CCAAATAACT 
AGAGGAATTG CAGCAAGGAG TTATGGATTA AATGTGGCTA AACTAGCAGA TGTTCCTGGA 
GAAATTTTGA AGAAAGCAGC TCACAAGTCA AAAGAGCTGG AAGGATTAAT AAATACGAAA 
AGAAAGAGAC TCAAGTATTT TGCAAAGTTA TGGACGATGC ATAATGCACA AGACCTGCAG 
AAGTGGACAG AGGAGTTCAA CATGGAAGAA ACACAGACTT CTCTTCTTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002430.3
CDS	81..3494
Translation	MSRRKPASGGLAASSSAPARQAVLSRFFQSTGSLKSTSSSTGAADQVDPGAAAAAAAAAAAAPPAPPAPAFPPQLPPHIATEIDRRKKRPLENDGPVKKKVKKVQQKEGGSDLGMSGNSEPKKCLRTRNVSKSLEKLKEFCCDSALPQSRVQTESLQERFAVLPKCTDFDDISLLHAKNAVSSEDSKRQINQKDTTLFDLSQFGSSNTSHENLQKTASKSANKRSKSIYTPLELQYIEMKQQHKDAVLCVECGYKYRFFGEDAEIAARELNIYCHLDHNFMTASIPTHRLFVHVRRLVAKGYKVGVVKQTETAALKAIGDNRSSLFSRKLTALYTKSTLIGEDVNPLIKLDDAVNVDEIMTDTSTSYLLCISENKENVRDKKKGNIFIGIVGVQPATGEVVFDSFQDSASRSELETRMSSLQPVELLLPSALSEQTEALIHRATSVSVQDDRIRVERMDNIYFEYSHAFQAVTEFYAKDTVDIKGSQIISGIVNLEKPVICSLAAIIKYLKEFNLEKMLSKPENFKQLSSKMEFMTINGTTLRNLEILQNQTDMKTKGSLLWVLDHTKTSFGRRKLKKWVTQPLLKLREINARLDAVSEVLHSESSVFGQIENHLRKLPDIERGLCSIYHKKCSTQEFFLIVKTLYHLKSEFQAIIPAVNSHIQSDLLRTVILEIPELLSPVEHYLKILNEQAAKVGDKTELFKDLSDFPLIKKRKDEIQGVIDEIRMHLQEIRKILKNPSAQYVTVSGQEFMIEIKNSAVSCIPTDWVKVGSTKAVSRFHSPFIVENYRHLNQLREQLVLDCSAEWLDFLEKFSEHYHSLCKAVHHLATVDCIFSLAKVAKQGDYCRPTVQEERKIVIKNGRHPVIDVLLGEQDQYVPNNTDLSEDSERVMIITGPNMGGKSSYIKQVALITIMAQIGSYVPAEEATIGIVDGIFTRMGAADNIYKGQSTFMEELTDTAEIIRKATSQSLVILDELGRGTSTHDGIAIAYATLEYFIRDVKSLTLFVTHYPPVCELEKNYSHQVGNYHMGFLVSEDESKLDPGAAEQVPDFVTFLYQITRGIAARSYGLNVAKLADVPGEILKKAAHKSKELEGLINTKRKRLKYFAKLWTMHNAQDLQKWTEEFNMEETQTSLLH

Target ORF information:

RefSeq Version	NM_002439.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mutS homolog 3 (MSH3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002439.4

ORF Insert Sequence:

ATGTCTCGCC GGAAGCCTGC GTCGGGCGGC CTCGCTGCCT CCAGCTCAGC CCCTGCGAGG 
CAAGCGGTTT TGAGCCGATT CTTCCAGTCT ACGGGAAGCC TGAAATCCAC CTCCTCCTCC 
ACAGGTGCAG CCGACCAGGT GGACCCTGGC GCTGCAGCGG CTGCAGCGGC CGCAGCGGCC 
GCAGCGCCCC CAGCGCCCCC AGCTCCCGCC TTCCCGCCCC AGCTGCCGCC GCACATAGCT 
ACAGAAATTG ACAGAAGAAA GAAGAGACCA TTGGAAAATG ATGGGCCTGT TAAAAAGAAA 
GTAAAGAAAG TCCAACAAAA GGAAGGAGGA AGTGATCTGG GAATGTCTGG CAACTCTGAG 
CCAAAGAAAT GTCTGAGGAC CAGGAATGTT TCAAAGTCTC TGGAAAAATT GAAAGAATTC 
TGCTGCGATT CTGCCCTTCC TCAAAGTAGA GTCCAGACAG AATCTCTGCA GGAGAGATTT 
GCAGTTCTGC CAAAATGTAC TGATTTTGAT GATATCAGTC TTCTACACGC AAAGAATGCA 
GTTTCTTCTG AAGATTCGAA ACGTCAAATT AATCAAAAGG ACACAACACT TTTTGATCTC 
AGTCAGTTTG GATCATCAAA TACAAGTCAT GAAAATTTAC AGAAAACTGC TTCCAAATCA 
GCTAACAAAC GGTCCAAAAG CATCTATACG CCGCTAGAAT TACAATACAT AGAAATGAAG 
CAGCAGCACA AAGATGCAGT TTTGTGTGTG GAATGTGGAT ATAAGTATAG ATTCTTTGGG 
GAAGATGCAG AGATTGCAGC CCGAGAGCTC AATATTTATT GCCATTTAGA TCACAACTTT 
ATGACAGCAA GTATACCTAC TCACAGACTG TTTGTTCATG TACGCCGCCT GGTGGCAAAA 
GGATATAAGG TGGGAGTTGT GAAGCAAACT GAAACTGCAG CATTAAAGGC CATTGGAGAC 
AACAGAAGTT CACTCTTTTC CCGGAAATTG ACTGCCCTTT ATACAAAATC TACACTTATT 
GGAGAAGATG TGAATCCCCT AATCAAGCTG GATGATGCTG TAAATGTTGA TGAGATAATG 
ACTGATACTT CTACCAGCTA TCTTCTGTGC ATCTCTGAAA ATAAGGAAAA TGTTAGGGAC 
AAAAAAAAGG GCAACATTTT TATTGGCATT GTGGGAGTGC AGCCTGCCAC AGGCGAGGTT 
GTGTTTGATA GTTTCCAGGA CTCTGCTTCT CGTTCAGAGC TAGAAACCCG GATGTCAAGC 
CTGCAGCCAG TAGAGCTGCT GCTTCCTTCG GCCTTGTCCG AGCAAACAGA GGCGCTCATC 
CACAGAGCCA CATCTGTTAG TGTGCAGGAT GACAGAATTC GAGTCGAAAG GATGGATAAC 
ATTTATTTTG AATACAGCCA TGCTTTCCAG GCAGTTACAG AGTTTTATGC AAAAGATACA 
GTTGACATCA AAGGTTCTCA AATTATTTCT GGCATTGTTA ACTTAGAGAA GCCTGTGATT 
TGCTCTTTGG CTGCCATCAT AAAATACCTC AAAGAATTCA ACTTGGAAAA GATGCTCTCC 
AAACCTGAGA ATTTTAAACA GCTATCAAGT AAAATGGAAT TTATGACAAT TAATGGAACA 
ACATTAAGGA ATCTGGAAAT CCTACAGAAT CAGACTGATA TGAAAACCAA AGGAAGTTTG 
CTGTGGGTTT TAGACCACAC TAAAACTTCA TTTGGGAGAC GGAAGTTAAA GAAGTGGGTG 
ACCCAGCCAC TCCTTAAATT AAGGGAAATA AATGCCCGGC TTGATGCTGT ATCGGAAGTT 
CTCCATTCAG AATCTAGTGT GTTTGGTCAG ATAGAAAATC ATCTACGTAA ATTGCCCGAC 
ATAGAGAGGG GACTCTGTAG CATTTATCAC AAAAAATGTT CTACCCAAGA GTTCTTCTTG 
ATTGTCAAAA CTTTATATCA CCTAAAGTCA GAATTTCAAG CAATAATACC TGCTGTTAAT 
TCCCACATTC AGTCAGACTT GCTCCGGACC GTTATTTTAG AAATTCCTGA ACTCCTCAGT 
CCAGTGGAGC ATTACTTAAA GATACTCAAT GAACAAGCTG CCAAAGTTGG GGATAAAACT 
GAATTATTTA AAGACCTTTC TGACTTCCCT TTAATAAAAA AGAGGAAGGA TGAAATTCAA 
GGTGTTATTG ACGAGATCCG AATGCATTTG CAAGAAATAC GAAAAATACT AAAAAATCCT 
TCTGCACAAT ATGTGACAGT ATCAGGACAG GAGTTTATGA TAGAAATAAA GAACTCTGCT 
GTATCTTGTA TACCAACTGA TTGGGTAAAG GTTGGAAGCA CAAAAGCTGT GAGCCGCTTT 
CACTCTCCTT TTATTGTAGA AAATTACAGA CATCTGAATC AGCTCCGGGA GCAGCTAGTC 
CTTGACTGCA GTGCTGAATG GCTTGATTTT CTAGAGAAAT TCAGTGAACA TTATCACTCC 
TTGTGTAAAG CAGTGCATCA CCTAGCAACT GTTGACTGCA TTTTCTCCCT GGCCAAGGTC 
GCTAAGCAAG GAGATTACTG CAGACCAACT GTACAAGAAG AAAGAAAAAT TGTAATAAAA 
AATGGAAGGC ACCCTGTGAT TGATGTGTTG CTGGGAGAAC AGGATCAATA TGTCCCAAAT 
AATACAGATT TATCAGAGGA CTCAGAGAGA GTAATGATAA TTACCGGACC AAACATGGGT 
GGAAAGAGCT CCTACATAAA ACAAGTTGCA TTGATTACCA TCATGGCTCA GATTGGCTCC 
TATGTTCCTG CAGAAGAAGC GACAATTGGG ATTGTGGATG GCATTTTCAC AAGGATGGGT 
GCTGCAGACA ATATATATAA AGGACAGAGT ACATTTATGG AAGAACTGAC TGACACAGCA 
GAAATAATCA GAAAAGCAAC ATCACAGTCC TTGGTTATCT TGGATGAACT AGGAAGAGGG 
ACGAGCACTC ATGATGGAAT TGCCATTGCC TATGCTACAC TTGAGTATTT CATCAGAGAT 
GTGAAATCCT TAACCCTGTT TGTCACCCAT TATCCGCCAG TTTGTGAACT AGAAAAAAAT 
TACTCACACC AGGTGGGGAA TTACCACATG GGATTCTTGG TCAGTGAGGA TGAAAGCAAA 
CTGGATCCAG GCGCAGCAGA ACAAGTCCCT GATTTTGTCA CCTTCCTTTA CCAAATAACT 
AGAGGAATTG CAGCAAGGAG TTATGGATTA AATGTGGCTA AACTAGCAGA TGTTCCTGGA 
GAAATTTTGA AGAAAGCAGC TCACAAGTCA AAAGAGCTGG AAGGATTAAT AAATACGAAA 
AGAAAGAGAC TCAAGTATTT TGCAAAGTTA TGGACGATGC ATAATGCACA AGACCTGCAG 
AAGTGGACAG AGGAGTTCAA CATGGAAGAA ACACAGACTT CTCTTCTTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of MSH2 and MSH6 due to heterozygous germline defects in MSH3 and MSH6.
Familial cancer16(4)491-500(2017 Oct)
Morak M,K?sbauer S,Kerscher M,Laner A,Nissen AM,Benet-Pag?s A,Schackert HK,Keller G,Massdorf T,Holinski-Feder E

Identification of genetic variants associated with Huntington's disease progression: a genome-wide association study.
The Lancet. Neurology16(9)701-711(2017 09)
Hensman Moss DJ,Pardi?as AF,Langbehn D,Lo K,Leavitt BR,Roos R,Durr A,Mead S,,,Holmans P,Jones L,Tabrizi SJ

Inactivation of MSH3 by promoter methylation correlates with primary tumor stage in nasopharyngeal carcinoma.
International journal of molecular medicine40(3)673-678(2017 Sep)
Ni H,Jiang B,Zhou Z,Yuan X,Cao X,Huang G,Li Y

MutS? abundance and Msh3 ATP hydrolysis activity are important drivers of CTG?CAG repeat expansions.
Nucleic acids research45(17)10068-10078(2017 Sep)
Keogh N,Chan KY,Li GM,Lahue RS

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MSH3 cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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