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PEPD cDNA ORF clone, Homo sapiens(Human)

Gene Symbol PEPD
Entrez Gene ID 5184
Full Name peptidase D
Synonyms PROLIDASE
General protein information
Preferred Names

peptidase D

Names

xaa-Pro dipeptidase
X-Pro dipeptidase
aminoacyl-L-proline hydrolase
imidodipeptidase
proline dipeptidase
testicular tissue protein Li 138

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

19

19q13.11

Summary This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009].
Disorder MIM:

613230

Disorder Html: Prolidase deficiency, 170100 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000285.3 NP_000276.2 xaa-Pro dipeptidase isoform 1
NM_001166057.1 NP_001159529.1 xaa-Pro dipeptidase isoform 3
NM_001166056.1 NP_001159528.1 xaa-Pro dipeptidase isoform 2



Pan troglodytes (chimpanzee) PEPD XP_512574.3
Bos taurus (cattle) PEPD NP_001073787.2
Drosophila melanogaster (fruit fly) Dip-C NP_650192.1
Rattus norvegicus (Norway rat) Pepd NP_001009641.1
Gallus gallus (chicken) PEPD NP_001073185.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP004775 XP_318039.3
Oryza sativa (rice) Os02g0224400 NP_001046335.1
Caenorhabditis elegans (roundworm) K12C11.1 NP_490843.1
Mus musculus (house mouse) Pepd NP_032846.2
Danio rerio (zebrafish) pepd NP_944594.1
Arabidopsis thaliana (thale cress) AT4G29490 NP_194678.2
Xenopus tropicalis (tropical clawed frog) pepd NP_001008094.1
Macaca mulatta (rhesus monkey) PEPD XP_001108725.2
Homo sapiens (human) PEPD NP_000276.2
Canis lupus familiaris (dog) PEPD XP_533702.2


Related articles in PubMed

Flavivirus Antagonism of Type I Interferon Signaling Reveals Prolidase as a Regulator of IFNAR1 Surface Expression.
Lubick KJ, Robertson SJ, McNally KL, Freedman BA, Rasmussen AL, Taylor RT, Walts AD, Tsuruda S, Sakai M, Ishizuka M, Boer EF, Foster EC, Chiramel AI, Addison CB, Green R, Kastner DL, Katze MG, Holland SM, Forlino A, Freeman AF, Boehm M, Yoshii K, Best SM
Cell host & microbe18(1)61-74(2015 Jul)

Assessment of the correlation between serum prolidase and alpha-fetoprotein levels in patients with hepatocellular carcinoma.
Ilikhan SU, Bilici M, Sahin H, Akca AS, Can M, Oz II, Guven B, Buyukuysal MC, Ustundag Y
World journal of gastroenterology21(22)6999-7007(2015 Jun)

Prolidase activity and oxidative stress in patients with schizophrenia: a preliminary study.
Bahceci B, Bagcioglu E, Kokacya MH, Dilek AR, Bahceci I, Selek S
JPMA. The Journal of the Pakistan Medical Association65(2)131-5(2015 Feb)

Sex-specific association of the peptidase D gene rs731839 polymorphism and serum lipid levels in the Mulao and Han populations.
Lin QZ, Yin RX, Wu J, Guo T, Wang W, Sun JQ, Shi GY, Shen SW, Wu JZ, Pan SL
International journal of clinical and experimental pathology7(7)4156-72(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Prolidase activity decreases in patients with chronic obstructive pulmonary disease.
Title: The relationships among the levels of oxidative and antioxidative parameters, FEV1 and prolidase activity in COPD.

In ankylosing spondylitis, serum prolidase level was successful in measuring disease activity
Title: Serum prolidase level in ankylosing spondylitis: low serum levels as a new potential gold standard biomarker for disease activity.

there is an independent relationship of aortic prolidase activity and aortic prolidase immunostaining with aortic stiffness beta index in patients who underwent coronary artery bypass grafting
Title: Relationship Between Echocardiographically Evaluated Aortic Stiffness and Prolidase Activity in Aortic Tissue of Patients with Critical Coronary Artery Disease.

absence of prolidase activity in pterygium tissue indicates that there is no collagen turnover in this tissue
Title: Prolidase Enzyme Activity in Conjunctiva and Pterygium Tissues.

Prolidase was required for IFNAR1 maturation and accumulation, activation of IFNbeta-stimulated gene induction, and IFN-I-dependent viral control.
Title: Flavivirus Antagonism of Type I Interferon Signaling Reveals Prolidase as a Regulator of IFNAR1 Surface Expression.

The following PEPD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PEPD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu24790 NM_000285.3
Latest version!
Homo sapiens peptidase D (PEPD), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu24752 NM_001166056.1
Latest version!
Homo sapiens peptidase D (PEPD), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu25079 NM_001166057.1
Latest version!
Homo sapiens peptidase D (PEPD), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $251.30
$359.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu24790
Clone ID Related Accession (Same CDS sequence) NM_000285.3
Accession Version NM_000285.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-13
Organism Homo sapiens(Human)
Product xaa-Pro dipeptidase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395475.1, BC015027.1 and AW512991.1. This sequence is a reference standard in the RefSeqGene project. On Oct 6, 2009 this sequence version replaced NM_000285.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005451.1, BC015027.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159080, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC
ACCGGGGTCC TCTTCCGCCA GGAGTCCTTC TTTCACTGGG CGTTCGGTGT CACTGAGCCA
GGCTGCTATG GTGTCATCGA TGTTGACACT GGGAAGTCGA CCCTGTTTGT GCCCAGGCTT
CCTGCCAGCC ATGCCACCTG GATGGGAAAG ATCCATTCCA AGGAGCACTT CAAGGAGAAG
TATGCCGTGG ACGACGTCCA GTACGTAGAT GAGATTGCCA GCGTCCTGAC GTCACAGAAG
CCCTCTGTCC TCCTCACTTT GCGTGGCGTC AACACGGACA GCGGCAGTGT CTGCAGGGAG
GCCTCCTTTG ACGGCATCAG CAAGTTCGAA GTCAACAATA CCATTCTTCA CCCAGAGATC
GTTGAGTGCC GAGTGTTTAA GACGGATATG GAGCTGGAGG TTCTGCGCTA TACCAATAAA
ATCTCCAGCG AGGCCCACCG TGAGGTAATG AAGGCTGTAA AAGTGGGAAT GAAAGAATAT
GAGTTGGAAA GCCTCTTCGA GCACTACTGC TACTCCCGGG GCGGCATGCG CCACAGCTCC
TACACCTGCA TCTGCGGCAG TGGTGAGAAC TCAGCCGTGC TACACTACGG ACACGCCGGA
GCTCCCAACG ACCGAACGAT CCAGAATGGG GATATGTGCC TGTTCGACAT GGGCGGTGAG
TATTACTGCT TCGCTTCCGA CATCACCTGC TCCTTTCCCG CCAACGGCAA GTTCACTGCA
GACCAGAAGG CCGTCTATGA GGCAGTGCTG CGGAGCTCCC GTGCCGTCAT GGGTGCCATG
AAGCCAGGTG TCTGGTGGCC TGACATGCAC CGCCTGGCTG ACCGCATCCA CCTGGAGGAG
CTGGCCCACA TGGGCATCCT GAGCGGCAGC GTGGACGCCA TGGTCCAGGC TCACCTGGGG
GCCGTGTTTA TGCCTCACGG GCTTGGCCAC TTCCTGGGCA TTGACGTGCA CGACGTGGGA
GGCTACCCAG AGGGCGTGGA GCGCATCGAC GAGCCCGGCC TGCGGAGCCT GCGCACTGCA
CGGCACCTGC AGCCAGGCAT GGTGCTCACC GTGGAGCCGG GCATCTACTT CATCGACCAC
CTCCTGGATG AGGCCCTGGC GGACCCGGCC CGCGCCTCCT TCCTTAACCG CGAGGTCCTG
CAGCGCTTTC GCGGTTTTGG CGGGGTCCGC ATCGAGGAGG ACGTCGTGGT GACTGACAGC
GGCATAGAGC TGCTGACCTG CGTGCCCCGC ACTGTGGAAG AGATTGAAGC ATGCATGGCA
GGCTGTGACA AGGCCTTTAC CCCCTTCTCT GGCCCCAAGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000276.2
CDS134..1615
Misc Feature(1)113..115
Misc Feature(2)137..139
Misc Feature(3)185..598
Misc Feature(4)212..1576
Misc Feature(5)632..634
Misc Feature(6)707..1534
Misc Feature(7)order(896..898,959..961,992..994,1241..1243,1367..1369,1487..1489)
Exon (1)1..150
Exon (2)151..334
Exon (3)335..462
Exon (4)463..526
Exon (5)527..574
Exon (6)575..636
Exon (7)637..681
Exon (8)682..757
Exon (9)758..804
Exon (10)805..873
Exon (11)874..951
Exon (12)952..1100
Exon (13)1101..1285
Exon (14)1286..1477
Exon (15)1478..2010
Translation

Target ORF information:

RefSeq Version NM_000285.3
Organism Homo sapiens(Human)
Definition Homo sapiens peptidase D (PEPD), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000285.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC
ACCGGGGTCC TCTTCCGCCA GGAGTCCTTC TTTCACTGGG CGTTCGGTGT CACTGAGCCA
GGCTGCTATG GTGTCATCGA TGTTGACACT GGGAAGTCGA CCCTGTTTGT GCCCAGGCTT
CCTGCCAGCC ATGCCACCTG GATGGGAAAG ATCCATTCCA AGGAGCACTT CAAGGAGAAG
TATGCCGTGG ACGACGTCCA GTACGTAGAT GAGATTGCCA GCGTCCTGAC GTCACAGAAG
CCCTCTGTCC TCCTCACTTT GCGTGGCGTC AACACGGACA GCGGCAGTGT CTGCAGGGAG
GCCTCCTTTG ACGGCATCAG CAAGTTCGAA GTCAACAATA CCATTCTTCA CCCAGAGATC
GTTGAGTGCC GAGTGTTTAA GACGGATATG GAGCTGGAGG TTCTGCGCTA TACCAATAAA
ATCTCCAGCG AGGCCCACCG TGAGGTAATG AAGGCTGTAA AAGTGGGAAT GAAAGAATAT
GAGTTGGAAA GCCTCTTCGA GCACTACTGC TACTCCCGGG GCGGCATGCG CCACAGCTCC
TACACCTGCA TCTGCGGCAG TGGTGAGAAC TCAGCCGTGC TACACTACGG ACACGCCGGA
GCTCCCAACG ACCGAACGAT CCAGAATGGG GATATGTGCC TGTTCGACAT GGGCGGTGAG
TATTACTGCT TCGCTTCCGA CATCACCTGC TCCTTTCCCG CCAACGGCAA GTTCACTGCA
GACCAGAAGG CCGTCTATGA GGCAGTGCTG CGGAGCTCCC GTGCCGTCAT GGGTGCCATG
AAGCCAGGTG TCTGGTGGCC TGACATGCAC CGCCTGGCTG ACCGCATCCA CCTGGAGGAG
CTGGCCCACA TGGGCATCCT GAGCGGCAGC GTGGACGCCA TGGTCCAGGC TCACCTGGGG
GCCGTGTTTA TGCCTCACGG GCTTGGCCAC TTCCTGGGCA TTGACGTGCA CGACGTGGGA
GGCTACCCAG AGGGCGTGGA GCGCATCGAC GAGCCCGGCC TGCGGAGCCT GCGCACTGCA
CGGCACCTGC AGCCAGGCAT GGTGCTCACC GTGGAGCCGG GCATCTACTT CATCGACCAC
CTCCTGGATG AGGCCCTGGC GGACCCGGCC CGCGCCTCCT TCCTTAACCG CGAGGTCCTG
CAGCGCTTTC GCGGTTTTGG CGGGGTCCGC ATCGAGGAGG ACGTCGTGGT GACTGACAGC
GGCATAGAGC TGCTGACCTG CGTGCCCCGC ACTGTGGAAG AGATTGAAGC ATGCATGGCA
GGCTGTGACA AGGCCTTTAC CCCCTTCTCT GGCCCCAAGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24752
Clone ID Related Accession (Same CDS sequence) NM_001166056.1
Accession Version NM_001166056.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-13
Organism Homo sapiens(Human)
Product xaa-Pro dipeptidase isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395475.1, AK294619.1 and AW512991.1. Transcript Variant: This variant (2) lacks two alternate in-frame exons in the central coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK294619.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159080, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC
ACCGGGGTCC TCTTCCGCCA GGAGTCCTTC TTTCACTGGG CGTTCGGTGT CACTGAGCCA
GGCTGCTATG GTGTCATCGA TGTTGACACT GGGAAGTCGA CCCTGTTTGT GCCCAGGCTT
CCTGCCAGCC ATGCCACCTG GATGGGAAAG ATCCATTCCA AGGAGCACTT CAAGGAGAAG
TATGCCGTGG ACGACGTCCA GTACGTAGAT GAGATTGCCA GCGTCCTGAC GTCACAGAAG
CCCTCTGTCC TCCTCACTTT GCGTGGCGTC AACACGGACA GCGGCAGTGT CTGCAGGGAG
GCCTCCTTTG ACGGCATCAG CAAGTTCGAA GTCAACAATA CCATTCTTCA CCCAGAGATC
GTTGAGTGCC TCTTCGAGCA CTACTGCTAC TCCCGGGGCG GCATGCGCCA CAGCTCCTAC
ACCTGCATCT GCGGCAGTGG TGAGAACTCA GCCGTGCTAC ACTACGGACA CGCCGGAGCT
CCCAACGACC GAACGATCCA GAATGGGGAT ATGTGCCTGT TCGACATGGG CGGTGAGTAT
TACTGCTTCG CTTCCGACAT CACCTGCTCC TTTCCCGCCA ACGGCAAGTT CACTGCAGAC
CAGAAGGCCG TCTATGAGGC AGTGCTGCGG AGCTCCCGTG CCGTCATGGG TGCCATGAAG
CCAGGTGTCT GGTGGCCTGA CATGCACCGC CTGGCTGACC GCATCCACCT GGAGGAGCTG
GCCCACATGG GCATCCTGAG CGGCAGCGTG GACGCCATGG TCCAGGCTCA CCTGGGGGCC
GTGTTTATGC CTCACGGGCT TGGCCACTTC CTGGGCATTG ACGTGCACGA CGTGGGAGGC
TACCCAGAGG GCGTGGAGCG CATCGACGAG CCCGGCCTGC GGAGCCTGCG CACTGCACGG
CACCTGCAGC CAGGCATGGT GCTCACCGTG GAGCCGGGCA TCTACTTCAT CGACCACCTC
CTGGATGAGG CCCTGGCGGA CCCGGCCCGC GCCTCCTTCC TTAACCGCGA GGTCCTGCAG
CGCTTTCGCG GTTTTGGCGG GGTCCGCATC GAGGAGGACG TCGTGGTGAC TGACAGCGGC
ATAGAGCTGC TGACCTGCGT GCCCCGCACT GTGGAAGAGA TTGAAGCATG CATGGCAGGC
TGTGACAAGG CCTTTACCCC CTTCTCTGGC CCCAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001159528.1
CDS134..1492
Misc Feature(1)113..115
Misc Feature(2)185..598
Misc Feature(3)311..1438
Misc Feature(4)611..1411
Misc Feature(5)order(773..775,836..838,869..871,1118..1120,1244..1246,1364..1366)
Exon (1)1..150
Exon (2)151..334
Exon (3)335..462
Exon (4)463..526
Exon (5)527..574
Exon (6)575..636
Exon (7)637..681
Exon (8)682..750
Exon (9)751..828
Exon (10)829..977
Exon (11)978..1162
Exon (12)1163..1354
Exon (13)1355..1887
Translation

Target ORF information:

RefSeq Version NM_001166056.1
Organism Homo sapiens(Human)
Definition Homo sapiens peptidase D (PEPD), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166056.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC
ACCGGGGTCC TCTTCCGCCA GGAGTCCTTC TTTCACTGGG CGTTCGGTGT CACTGAGCCA
GGCTGCTATG GTGTCATCGA TGTTGACACT GGGAAGTCGA CCCTGTTTGT GCCCAGGCTT
CCTGCCAGCC ATGCCACCTG GATGGGAAAG ATCCATTCCA AGGAGCACTT CAAGGAGAAG
TATGCCGTGG ACGACGTCCA GTACGTAGAT GAGATTGCCA GCGTCCTGAC GTCACAGAAG
CCCTCTGTCC TCCTCACTTT GCGTGGCGTC AACACGGACA GCGGCAGTGT CTGCAGGGAG
GCCTCCTTTG ACGGCATCAG CAAGTTCGAA GTCAACAATA CCATTCTTCA CCCAGAGATC
GTTGAGTGCC TCTTCGAGCA CTACTGCTAC TCCCGGGGCG GCATGCGCCA CAGCTCCTAC
ACCTGCATCT GCGGCAGTGG TGAGAACTCA GCCGTGCTAC ACTACGGACA CGCCGGAGCT
CCCAACGACC GAACGATCCA GAATGGGGAT ATGTGCCTGT TCGACATGGG CGGTGAGTAT
TACTGCTTCG CTTCCGACAT CACCTGCTCC TTTCCCGCCA ACGGCAAGTT CACTGCAGAC
CAGAAGGCCG TCTATGAGGC AGTGCTGCGG AGCTCCCGTG CCGTCATGGG TGCCATGAAG
CCAGGTGTCT GGTGGCCTGA CATGCACCGC CTGGCTGACC GCATCCACCT GGAGGAGCTG
GCCCACATGG GCATCCTGAG CGGCAGCGTG GACGCCATGG TCCAGGCTCA CCTGGGGGCC
GTGTTTATGC CTCACGGGCT TGGCCACTTC CTGGGCATTG ACGTGCACGA CGTGGGAGGC
TACCCAGAGG GCGTGGAGCG CATCGACGAG CCCGGCCTGC GGAGCCTGCG CACTGCACGG
CACCTGCAGC CAGGCATGGT GCTCACCGTG GAGCCGGGCA TCTACTTCAT CGACCACCTC
CTGGATGAGG CCCTGGCGGA CCCGGCCCGC GCCTCCTTCC TTAACCGCGA GGTCCTGCAG
CGCTTTCGCG GTTTTGGCGG GGTCCGCATC GAGGAGGACG TCGTGGTGAC TGACAGCGGC
ATAGAGCTGC TGACCTGCGT GCCCCGCACT GTGGAAGAGA TTGAAGCATG CATGGCAGGC
TGTGACAAGG CCTTTACCCC CTTCTCTGGC CCCAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25079
Clone ID Related Accession (Same CDS sequence) NM_001166057.1
Accession Version NM_001166057.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1290bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-04-12
Organism Homo sapiens(Human)
Product xaa-Pro dipeptidase isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395475.1, AK293126.1, BC015027.1 and AW512991.1. Transcript Variant: This variant (3) lacks two alternate in-frame exons in the central coding region, compared to variant 1. The resulting isoform (3) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293126.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC
ACCGGGGTCC TCTTCCGCCA GATTGCCAGC GTCCTGACGT CACAGAAGCC CTCTGTCCTC
CTCACTTTGC GTGGCGTCAA CACGGACAGC GGCAGTGTCT GCAGGGAGGC CTCCTTTGAC
GGCATCAGCA AGTTCGAAGT CAACAATACC ATTCTTCACC CAGAGATCGT TGAGTGCCGA
GTGTTTAAGA CGGATATGGA GCTGGAGGTT CTGCGCTATA CCAATAAAAT CTCCAGCGAG
GCCCACCGTG AGGTAATGAA GGCTGTAAAA GTGGGAATGA AAGAATATGA GTTGGAAAGC
CTCTTCGAGC ACTACTGCTA CTCCCGGGGC GGCATGCGCC ACAGCTCCTA CACCTGCATC
TGCGGCAGTG GTGAGAACTC AGCCGTGCTA CACTACGGAC ACGCCGGAGC TCCCAACGAC
CGAACGATCC AGAATGGGGA TATGTGCCTG TTCGACATGG GCGGTGAGTA TTACTGCTTC
GCTTCCGACA TCACCTGCTC CTTTCCCGCC AACGGCAAGT TCACTGCAGA CCAGAAGGCC
GTCTATGAGG CAGTGCTGCG GAGCTCCCGT GCCGTCATGG GTGCCATGAA GCCAGGTGTC
TGGTGGCCTG ACATGCACCG CCTGGCTGAC CGCATCCACC TGGAGGAGCT GGCCCACATG
GGCATCCTGA GCGGCAGCGT GGACGCCATG GTCCAGGCTC ACCTGGGGGC CGTGTTTATG
CCTCACGGGC TTGGCCACTT CCTGGGCATT GACGTGCACG ACGTGGGAGG CTACCCAGAG
GGCGTGGAGC GCATCGACGA GCCCGGCCTG CGGAGCCTGC GCACTGCACG GCACCTGCAG
CCAGGCATGG TGCTCACCGT GGAGCCGGGC ATCTACTTCA TCGACCACCT CCTGGATGAG
GCCCTGGCGG ACCCGGCCCG CGCCTCCTTC CTTAACCGCG AGGTCCTGCA GCGCTTTCGC
GGTTTTGGCG GGGTCCGCAT CGAGGAGGAC GTCGTGGTGA CTGACAGCGG CATAGAGCTG
CTGACCTGCG TGCCCCGCAC TGTGGAAGAG ATTGAAGCAT GCATGGCAGG CTGTGACAAG
GCCTTTACCC CCTTCTCTGG CCCCAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001159529.1
CDS134..1423
Misc Feature(1)113..115
Misc Feature(2)137..139
Misc Feature(3)185..>334
Misc Feature(4)515..1342
Misc Feature(5)order(704..706,767..769,800..802,1049..1051,1175..1177,1295..1297)
Exon (1)1..150
Exon (2)151..334
Exon (3)335..382
Exon (4)383..444
Exon (5)445..489
Exon (6)490..565
Exon (7)566..612
Exon (8)613..681
Exon (9)682..759
Exon (10)760..908
Exon (11)909..1093
Exon (12)1094..1285
Exon (13)1286..1818
Translation

Target ORF information:

RefSeq Version NM_001166057.1
Organism Homo sapiens(Human)
Definition Homo sapiens peptidase D (PEPD), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166057.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGCGG CCACCGGACC CTCGTTTTGG CTGGGGAATG AAACCCTGAA GGTGCCGCTG 
GCGCTCTTTG CCTTGAACCG GCAGCGCCTG TGTGAGCGGC TGCGGAAGAA CCCTGCTGTG
CAGGCCGGCT CCATCGTGGT CCTGCAGGGC GGGGAGGAGA CTCAGCGCTA CTGCACCGAC
ACCGGGGTCC TCTTCCGCCA GATTGCCAGC GTCCTGACGT CACAGAAGCC CTCTGTCCTC
CTCACTTTGC GTGGCGTCAA CACGGACAGC GGCAGTGTCT GCAGGGAGGC CTCCTTTGAC
GGCATCAGCA AGTTCGAAGT CAACAATACC ATTCTTCACC CAGAGATCGT TGAGTGCCGA
GTGTTTAAGA CGGATATGGA GCTGGAGGTT CTGCGCTATA CCAATAAAAT CTCCAGCGAG
GCCCACCGTG AGGTAATGAA GGCTGTAAAA GTGGGAATGA AAGAATATGA GTTGGAAAGC
CTCTTCGAGC ACTACTGCTA CTCCCGGGGC GGCATGCGCC ACAGCTCCTA CACCTGCATC
TGCGGCAGTG GTGAGAACTC AGCCGTGCTA CACTACGGAC ACGCCGGAGC TCCCAACGAC
CGAACGATCC AGAATGGGGA TATGTGCCTG TTCGACATGG GCGGTGAGTA TTACTGCTTC
GCTTCCGACA TCACCTGCTC CTTTCCCGCC AACGGCAAGT TCACTGCAGA CCAGAAGGCC
GTCTATGAGG CAGTGCTGCG GAGCTCCCGT GCCGTCATGG GTGCCATGAA GCCAGGTGTC
TGGTGGCCTG ACATGCACCG CCTGGCTGAC CGCATCCACC TGGAGGAGCT GGCCCACATG
GGCATCCTGA GCGGCAGCGT GGACGCCATG GTCCAGGCTC ACCTGGGGGC CGTGTTTATG
CCTCACGGGC TTGGCCACTT CCTGGGCATT GACGTGCACG ACGTGGGAGG CTACCCAGAG
GGCGTGGAGC GCATCGACGA GCCCGGCCTG CGGAGCCTGC GCACTGCACG GCACCTGCAG
CCAGGCATGG TGCTCACCGT GGAGCCGGGC ATCTACTTCA TCGACCACCT CCTGGATGAG
GCCCTGGCGG ACCCGGCCCG CGCCTCCTTC CTTAACCGCG AGGTCCTGCA GCGCTTTCGC
GGTTTTGGCG GGGTCCGCAT CGAGGAGGAC GTCGTGGTGA CTGACAGCGG CATAGAGCTG
CTGACCTGCG TGCCCCGCAC TGTGGAAGAG ATTGAAGCAT GCATGGCAGG CTGTGACAAG
GCCTTTACCC CCTTCTCTGG CCCCAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Flavivirus Antagonism of Type I Interferon Signaling Reveals Prolidase as a Regulator of IFNAR1 Surface Expression.
Cell host & microbe18(1)61-74(2015 Jul)
Lubick KJ,Robertson SJ,McNally KL,Freedman BA,Rasmussen AL,Taylor RT,Walts AD,Tsuruda S,Sakai M,Ishizuka M,Boer EF,Foster EC,Chiramel AI,Addison CB,Green R,Kastner DL,Katze MG,Holland SM,Forlino A,Freeman AF,Boehm M,Yoshii K,Best SM


book

Assessment of the correlation between serum prolidase and alpha-fetoprotein levels in patients with hepatocellular carcinoma.
World journal of gastroenterology21(22)6999-7007(2015 Jun)
Ilikhan SU,Bilici M,Sahin H,Akca AS,Can M,Oz II,Guven B,Buyukuysal MC,Ustundag Y


book

Prolidase activity and oxidative stress in patients with schizophrenia: a preliminary study.
JPMA. The Journal of the Pakistan Medical Association65(2)131-5(2015 Feb)
Bahceci B,Bagcioglu E,Kokacya MH,Dilek AR,Bahceci I,Selek S


book

Sex-specific association of the peptidase D gene rs731839 polymorphism and serum lipid levels in the Mulao and Han populations.
International journal of clinical and experimental pathology7(7)4156-72(2014)
Lin QZ,Yin RX,Wu J,Guo T,Wang W,Sun JQ,Shi GY,Shen SW,Wu JZ,Pan SL


 
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