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BCL2 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol BCL2
Entrez Gene ID 596
Full Name BCL2, apoptosis regulator
Synonyms Bcl-2,PPP1R50
General protein information
Preferred Names

BCL2, apoptosis regulator

Names

apoptosis regulator Bcl-2
B-cell CLL/lymphoma 2
protein phosphatase 1, regulatory subunit 50

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

18

18q21.33

Summary This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016].
Disorder MIM:

151430

Disorder Html: Leukemia/lymphoma, B-cell, 2 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011526135.2 XP_011524437.1 apoptosis regulator Bcl-2 isoform X1
XM_017025917.1 XP_016881406.1 apoptosis regulator Bcl-2 isoform X2
NM_000657.2 NP_000648.2 apoptosis regulator Bcl-2 beta isoform
NM_000633.2 NP_000624.2 apoptosis regulator Bcl-2 alpha isoform



Homo sapiens (human) BCL2 NP_000624.2
Pan troglodytes (chimpanzee) BCL2 XP_001145537.1
Canis lupus familiaris (dog) BCL2 NP_001002949.1
Xenopus tropicalis (tropical clawed frog) bcl2 XP_002934442.1
Bos taurus (cattle) BCL2 NP_001159958.1
Rattus norvegicus (Norway rat) Bcl2 NP_058689.1
Gallus gallus (chicken) BCL2 NP_990670.1
Mus musculus (house mouse) Bcl2 NP_033871.2


Related articles in PubMed

MicroRNA-190b confers radio-sensitivity through negative regulation of Bcl-2 in gastric cancer cells.
Wang C, Qiao C
Biotechnology letters39(4)485-490(2017 Apr)

B-cell lymphoma 2 inhibitor ABT-737 induces Beclin1- and reactive oxygen species-dependent autophagy in Adriamycin-resistant human hepatocellular carcinoma cells.
Yao X, Li X, Zhang D, Xie Y, Sun B, Li H, Sun L, Zhang X
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(3)1010428317695965(2017 Mar)

Bcl-2 overexpression contributes to laryngeal carcinoma cell survival by forming a complex with Hsp90β.
Li S, Li J, Hu T, Zhang C, Lv X, He S, Yan H, Tan Y, Wen M, Lei M, Zuo J
Oncology reports37(2)849-856(2017 Feb)

Potential role of BCL2 in the recurrence of uterine smooth muscle tumors of uncertain malignant potential.
Conconi D, Chiappa V, Perego P, Redaelli S, Bovo G, Lavitrano M, Milani R, Dalprà L, Lissoni AA
Oncology reports37(1)41-47(2017 Jan)

Mutational analysis of Bax and Bcl-2 in childhood acute lymphoblastic leukaemia.
Salomons GS, Buitenhuis CK, Martínez Muñoz C, Verwijs-Jassen M, Behrendt H, Zsiros J, Smets LA
International journal of cancer79(3)273-7(1998 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bcl-2 regulates the inositol 1,4,5-trisphosphate receptor and calcium signaling in normal and malignant lymphocytes. (Review)
Title: Bcl-2 regulation of the inositol 1,4,5-trisphosphate receptor and calcium signaling in normal and malignant lymphocytes: potential new target for cancer treatment.

the stability of different G-quadruplex folds of the 23-mer bcl2 promoter region and G-quadruplex ligand, is reported.
Title: Relative stability of G-quadruplex structures: Interactions between the human Bcl2 promoter region and derivatives of carbazole and diphenylamine.

Many players in BCL-2 family affairs. [review]
Title: Many players in BCL-2 family affairs.

A structural basis for a conserved binding mechanism between p53DBD and the anti-apoptotic Bcl-2 family proteins.
Title: A conserved mechanism for binding of p53 DNA-binding domain and anti-apoptotic Bcl-2 family proteins.

Negative expression of bcl-2 is associated with recurrence after curative resection in gastric carcinoma.
Title: Prediction of tumor recurrence after curative resection in gastric carcinoma based on bcl-2 expression.

The following BCL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu25505 NM_000633.2
Latest version!
Homo sapiens BCL2, apoptosis regulator (BCL2), transcript variant alpha, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu88004 XM_017025917.1
Latest version!
Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $69.30
$99.00
OHu25321 NM_000657.2
Latest version!
Homo sapiens BCL2, apoptosis regulator (BCL2), transcript variant beta, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $69.30
$99.00
OHu51275 XM_011526135.2
Latest version!
Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $139.30
$199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25505
Clone ID Related Accession (Same CDS sequence) NM_000633.2
Accession Version NM_000633.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 720bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product apoptosis regulator Bcl-2 alpha isoform
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC021803.8, BC027258.1 and AC022726.13. This sequence is a reference standard in the RefSeqGene project. On Aug 10, 2005 this sequence version replaced NM_000633.1. Transcript Variant: This variant (alpha) represents the longer transcript and encodes the longer isoform (alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.104374.1, SRR1803615.181714.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta regulatory uORF :: PMID: 9645350 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGGATGC CTTTGTGGAA
CTGTACGGCC CCAGCATGCG GCCTCTGTTT GATTTCTCCT GGCTGTCTCT GAAGACTCTG
CTCAGTTTGG CCCTGGTGGG AGCTTGCATC ACCCTGGGTG CCTATCTGGG CCACAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000624.2
CDS494..1213
Misc Feature(1)375..410
Misc Feature(2)392..394
Misc Feature(3)494..1210
Misc Feature(4)512..592
Misc Feature(5)521..583
Misc Feature(6)563..565
Misc Feature(7)593..598
Misc Feature(8)593..595
Misc Feature(9)659..661
Misc Feature(10)698..700
Misc Feature(11)698..700
Misc Feature(12)701..703
Misc Feature(13)701..703
Misc Feature(14)701..703
Misc Feature(15)713..715
Misc Feature(16)749..1096
Misc Feature(17)752..754
Misc Feature(18)752..754
Misc Feature(19)752..754
Misc Feature(20)770..814
Misc Feature(21)782..808
Misc Feature(22)order(791..796,800..805,815..817,824..829,875..880,887..892,899..904,920..922,926..931,935..940,950..952,962..964,1094..1096)
Misc Feature(23)899..958
Misc Feature(24)order(899..907,917..955)
Misc Feature(25)1052..1099
Misc Feature(26)1052..1096
Misc Feature(27)1127..1192
Exon (1)1..207
Exon (2)208..1078
Exon (3)1079..6492
Translation

Target ORF information:

RefSeq Version NM_000633.2
Organism Homo sapiens(Human)
Definition Homo sapiens BCL2, apoptosis regulator (BCL2), transcript variant alpha, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000633.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGGATGC CTTTGTGGAA
CTGTACGGCC CCAGCATGCG GCCTCTGTTT GATTTCTCCT GGCTGTCTCT GAAGACTCTG
CTCAGTTTGG CCCTGGTGGG AGCTTGCATC ACCCTGGGTG CCTATCTGGG CCACAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu88004
Clone ID Related Accession (Same CDS sequence) XM_017025917.1
Accession Version XM_017025917.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 597bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product apoptosis regulator Bcl-2 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGCTTTA CATGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016881406.1
CDS892..1488
Translation

Target ORF information:

RefSeq Version XM_017025917.1
Organism Homo sapiens(Human)
Definition Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017025917.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGCTTTA CATGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25321
Clone ID Related Accession (Same CDS sequence) NM_000657.2
Accession Version NM_000657.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 618bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product apoptosis regulator Bcl-2 beta isoform
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC021803.8 and AI401297.1. This sequence is a reference standard in the RefSeqGene project. On Aug 10, 2005 this sequence version replaced NM_000657.1. Transcript Variant: This variant (beta) differs in the 3' UTR and coding region compared to variant alpha. The resulting isoform (beta) is shorter and has a distinct C-terminus compared to isoform alpha. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.381699.1, SRR1163657.449367.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta regulatory uORF :: PMID: 9645350 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGGTAGG TGCACTTGGT
GATGTGAGTC TGGGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000648.2
CDS494..1111
Misc Feature(1)375..410
Misc Feature(2)392..394
Misc Feature(3)494..>1078
Misc Feature(4)512..592
Misc Feature(5)521..583
Misc Feature(6)563..565
Misc Feature(7)593..598
Misc Feature(8)593..595
Misc Feature(9)659..661
Misc Feature(10)698..700
Misc Feature(11)698..700
Misc Feature(12)701..703
Misc Feature(13)701..703
Misc Feature(14)713..715
Misc Feature(15)749..1084
Misc Feature(16)752..754
Misc Feature(17)752..754
Misc Feature(18)752..754
Misc Feature(19)752..754
Misc Feature(20)770..814
Misc Feature(21)782..808
Misc Feature(22)order(791..796,800..805,815..817,824..829,875..880,887..892,899..904,920..922,926..931,935..940,950..952,962..964)
Misc Feature(23)899..958
Misc Feature(24)order(899..907,917..955)
Exon (1)1..207
Exon (2)208..1206
Translation

Target ORF information:

RefSeq Version NM_000657.2
Organism Homo sapiens(Human)
Definition Homo sapiens BCL2, apoptosis regulator (BCL2), transcript variant beta, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000657.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGGTAGG TGCACTTGGT
GATGTGAGTC TGGGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu51275
Clone ID Related Accession (Same CDS sequence) XM_011526135.2
Accession Version XM_011526135.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 675bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product apoptosis regulator Bcl-2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011526135.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGGATGC CCTTGTGTTA
TTTGCAGGGC TAAGGGAGTT TCAGCATGGG CCAGGAAACT ACTTTCTTAA AAGAAACTTA
AACGCGAAAT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011524437.1
CDS1113..1787
Misc Feature(1)1113..1721
Misc Feature(2)1131..1211
Misc Feature(3)1368..1715
Misc Feature(4)1401..1427
Misc Feature(5)order(1410..1415,1419..1424,1434..1436,1443..1448,1494..1499,1506..1511,1518..1523,1539..1541,1545..1550,1554..1559,1569..1571,1581..1583,1713..1715)
Misc Feature(6)order(1518..1526,1536..1574)
Misc Feature(7)1671..1715
Translation

Target ORF information:

RefSeq Version XM_011526135.2
Organism Homo sapiens(Human)
Definition Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011526135.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGGATGC CCTTGTGTTA
TTTGCAGGGC TAAGGGAGTT TCAGCATGGG CCAGGAAACT ACTTTCTTAA AAGAAACTTA
AACGCGAAAT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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MicroRNA-190b confers radio-sensitivity through negative regulation of Bcl-2 in gastric cancer cells.
Biotechnology letters39(4)485-490(2017 Apr)
Wang C,Qiao C


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B-cell lymphoma 2 inhibitor ABT-737 induces Beclin1- and reactive oxygen species-dependent autophagy in Adriamycin-resistant human hepatocellular carcinoma cells.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(3)1010428317695965(2017 Mar)
Yao X,Li X,Zhang D,Xie Y,Sun B,Li H,Sun L,Zhang X


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Bcl-2 overexpression contributes to laryngeal carcinoma cell survival by forming a complex with Hsp90β.
Oncology reports37(2)849-856(2017 Feb)
Li S,Li J,Hu T,Zhang C,Lv X,He S,Yan H,Tan Y,Wen M,Lei M,Zuo J


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Potential role of BCL2 in the recurrence of uterine smooth muscle tumors of uncertain malignant potential.
Oncology reports37(1)41-47(2017 Jan)
Conconi D,Chiappa V,Perego P,Redaelli S,Bovo G,Lavitrano M,Milani R,Dalprà L,Lissoni AA


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Mutational analysis of Bax and Bcl-2 in childhood acute lymphoblastic leukaemia.
International journal of cancer79(3)273-7(1998 Jun)
Salomons GS,Buitenhuis CK,Martínez Muñoz C,Verwijs-Jassen M,Behrendt H,Zsiros J,Smets LA


 
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