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SRY cDNA ORF clone, Homo sapiens(Human)

Gene Symbol SRY
Entrez Gene ID 6736
Full Name sex determining region Y
Synonyms SRXX1,SRXY1,TDF,TDY
General protein information
Preferred Names

sex determining region Y

Names

sex-determining region Y protein
essential protein for sex determination in human males
sex-determining region on Y
testis-determining factor on Y
truncated sex-determining region Y protein

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

Y

Yp11.2

Summary This intronless gene encodes a transcription factor that is a member of the high mobility group (HMG)-box family of DNA-binding proteins. This protein is the testis-determining factor (TDF), which initiates male sex determination. Mutations in this gene give rise to XY females with gonadal dysgenesis (Swyer syndrome); translocation of part of the Y chromosome containing this gene to the X chromosome causes XX male syndrome. [provided by RefSeq, Jul 2008].
Disorder MIM:

480000

Disorder Html: 46XY complete gonadal dysgenesis, 400044 (3); 46XX true

mRNA and Protein(s)

mRNA Protein Name
NM_003140.2 NP_003131.1 sex-determining region Y protein



Bos taurus (cattle) SRY NP_001014407.1
Pan troglodytes (chimpanzee) SRY NP_001008988.1
Homo sapiens (human) SRY NP_003131.1


Related articles in PubMed

A Novel Missense Mutation 224G>T (R75M) in SRY Coding Region Interferes with Nuclear Import and Results in 46, XY Complete Gonadal Dysgenesis.
Fan W, Wang B, He S, Zhang T, Yin C, Chen Y, Zheng S, Zhang J, Li L
PloS one11(12)e0168484(2016)

Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans.
Calvo SE, Pagliarini DJ, Mootha VK
Proceedings of the National Academy of Sciences of the United States of America106(18)7507-12(2009 May)

Sex determination and SRY: down to a wink and a nudge?
Sekido R, Lovell-Badge R
Trends in genetics : TIG25(1)19-29(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a single-nucleotide polymorphism in the amelogenin gene using amplified product-length polymorphisms in combination with sex-determining region Y analysis, is reported.
Title: Sex Determination from Fragmented and Degenerated DNA by Amplified Product-Length Polymorphism Bidirectional SNP Analysis of Amelogenin and SRY Genes.

Chemical proteasome inhibition restored native-like SRY expression and transcriptional activity in association with restored occupancy of a sex-specific enhancer element in principal downstream gene Sox9, demonstrating that the variant SRY exhibits essentially native activity on a per molecule basis.
Title: Human Sex Determination at the Edge of Ambiguity: INHERITED XY SEX REVERSAL DUE TO ENHANCED UBIQUITINATION AND PROTEASOMAL DEGRADATION OF A MASTER TRANSCRIPTION FACTOR.

Missense Mutation 224G>T (R75M) in SRY Coding Region is associated with 46, XY Gonadal Dysgenesis.
Title: A Novel Missense Mutation 224G>T (R75M) in SRY Coding Region Interferes with Nuclear Import and Results in 46, XY Complete Gonadal Dysgenesis.

SRY is involved in the maintenance of cancer stem-like characteristics through OCT4.
Title: SRY and OCT4 Are Required for the Acquisition of Cancer Stem Cell-Like Properties and Are Potential Differentiation Therapy Targets.

The aim of this study was to compare two Y chromosome markers-SRY and DYS14-for their utility in the diagnosis of fetal gender
Title: Noninvasive Diagnosis of Fetal Gender: Utility of Combining DYS14 and SRY.

The following SRY gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SRY cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OHu28314 NM_003140.2
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Homo sapiens sex determining region Y (SRY), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu28314
Clone ID Related Accession (Same CDS sequence) NM_003140.2
Accession Version NM_003140.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 615bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-15
Organism Homo sapiens(Human)
Product sex-determining region Y protein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006040.3 and L10101.1. This sequence is a reference standard in the RefSeqGene project. On Jul 2, 2013 this sequence version replaced NM_003140.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
ATGCAATCAT ATGCTTCTGC TATGTTAAGC GTATTCAACA GCGATGATTA CAGTCCAGCT 
GTGCAAGAGA ATATTCCCGC TCTCCGGAGA AGCTCTTCCT TCCTTTGCAC TGAAAGCTGT
AACTCTAAGT ATCAGTGTGA AACGGGAGAA AACAGTAAAG GCAACGTCCA GGATAGAGTG
AAGCGACCCA TGAACGCATT CATCGTGTGG TCTCGCGATC AGAGGCGCAA GATGGCTCTA
GAGAATCCCA GAATGCGAAA CTCAGAGATC AGCAAGCAGC TGGGATACCA GTGGAAAATG
CTTACTGAAG CCGAAAAATG GCCATTCTTC CAGGAGGCAC AGAAATTACA GGCCATGCAC
AGAGAGAAAT ACCCGAATTA TAAGTATCGA CCTCGTCGGA AGGCGAAGAT GCTGCCGAAG
AATTGCAGTT TGCTTCCCGC AGATCCCGCT TCGGTACTCT GCAGCGAAGT GCAACTGGAC
AACAGGTTGT ACAGGGATGA CTGTACGAAA GCCACACACT CAAGAATGGA GCACCAGCTA
GGCCACTTAC CGCCCATCAA CGCAGCCAGC TCACCGCAGC AACGGGACCG CTACAGCCAC
TGGACAAAGC TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003131.1
CDS139..753
Misc Feature(1)1
Misc Feature(2)79..81
Misc Feature(3)313..546
Misc Feature(4)313..528
Misc Feature(5)319..369
Misc Feature(6)order(322..324,328..333,337..342,349..354,361..363,373..375,397..399,412..414,421..423,430..432,487..489)
Misc Feature(7)457..555
Misc Feature(8)523..732
Misc Feature(9)526..546
Misc Feature(10)544..546
Misc Feature(11)550..603
Misc Feature(12)730..750
Exon (1)1..887
Translation

Target ORF information:

RefSeq Version NM_003140.2
Organism Homo sapiens(Human)
Definition Homo sapiens sex determining region Y (SRY), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003140.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGCAATCAT ATGCTTCTGC TATGTTAAGC GTATTCAACA GCGATGATTA CAGTCCAGCT 
GTGCAAGAGA ATATTCCCGC TCTCCGGAGA AGCTCTTCCT TCCTTTGCAC TGAAAGCTGT
AACTCTAAGT ATCAGTGTGA AACGGGAGAA AACAGTAAAG GCAACGTCCA GGATAGAGTG
AAGCGACCCA TGAACGCATT CATCGTGTGG TCTCGCGATC AGAGGCGCAA GATGGCTCTA
GAGAATCCCA GAATGCGAAA CTCAGAGATC AGCAAGCAGC TGGGATACCA GTGGAAAATG
CTTACTGAAG CCGAAAAATG GCCATTCTTC CAGGAGGCAC AGAAATTACA GGCCATGCAC
AGAGAGAAAT ACCCGAATTA TAAGTATCGA CCTCGTCGGA AGGCGAAGAT GCTGCCGAAG
AATTGCAGTT TGCTTCCCGC AGATCCCGCT TCGGTACTCT GCAGCGAAGT GCAACTGGAC
AACAGGTTGT ACAGGGATGA CTGTACGAAA GCCACACACT CAAGAATGGA GCACCAGCTA
GGCCACTTAC CGCCCATCAA CGCAGCCAGC TCACCGCAGC AACGGGACCG CTACAGCCAC
TGGACAAAGC TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A Novel Missense Mutation 224G>T (R75M) in SRY Coding Region Interferes with Nuclear Import and Results in 46, XY Complete Gonadal Dysgenesis.
PloS one11(12)e0168484(2016)
Fan W,Wang B,He S,Zhang T,Yin C,Chen Y,Zheng S,Zhang J,Li L


book

Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans.
Proceedings of the National Academy of Sciences of the United States of America106(18)7507-12(2009 May)
Calvo SE,Pagliarini DJ,Mootha VK


book

Sex determination and SRY: down to a wink and a nudge?
Trends in genetics : TIG25(1)19-29(2009 Jan)
Sekido R,Lovell-Badge R


 
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