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TNF cDNA ORF clone, Homo sapiens(Human)

Gene Symbol TNF
Entrez Gene ID 7124
Full Name tumor necrosis factor
Synonyms DIF,TNF-alpha,TNFA,TNFSF2,TNLG1F
General protein information
Preferred Names

tumor necrosis factor

Names

tumor necrosis factor
APC1 protein
TNF, macrophage-derived
TNF, monocyte-derived
TNF-a
cachectin
tumor necrosis factor ligand 1F
tumor necrosis factor ligand superfamily member 2
tumor necrosis factor-alpha

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

6

6p21.33

Summary This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008].
Disorder MIM:

191160

Disorder Html: {Malaria, cerebral, susceptibility to}, 611162 (3); {Septic shock,

mRNA and Protein(s)

mRNA Protein Name
NM_000594.3 NP_000585.2 tumor necrosis factor



Mus musculus (house mouse) Tnf NP_038721.1
Canis lupus familiaris (dog) TNF NP_001003244.4
Bos taurus (cattle) TNF NP_776391.2
Rattus norvegicus (Norway rat) Tnf NP_036807.1
Xenopus tropicalis (tropical clawed frog) tnf NP_001107143.1
Macaca mulatta (rhesus monkey) TNF NP_001040614.1
Homo sapiens (human) TNF NP_000585.2
Pan troglodytes (chimpanzee) TNF NP_001038976.1


Related articles in PubMed

Impaired NK cell functionality and increased TNF-α production as biomarkers of chronic chikungunya arthritis and rheumatoid arthritis.
Thanapati S, Ganu M, Giri P, Kulkarni S, Sharma M, Babar P, Ganu A, Tripathy AS
Human immunology78(4)370-374(2017 Apr)

IL-21 Is Positively Associated with Intervertebral Disc Degeneration by Interaction with TNF-α Through the JAK-STAT Signaling Pathway.
Chen B, Liu Y, Zhang Y, Li J, Cheng K, Cheng L
Inflammation40(2)612-622(2017 Apr)

Tumor necrosis factor-alpha gene polymorphisms and susceptibility to ischemic heart disease: A systematic review and meta-analysis.
Zhang P, Wu X, Li G, He Q, Dai H, Ai C, Shi J
Medicine96(14)e6569(2017 Apr)

Clinical Relevance of Cytokines Gene Polymorphisms and Protein Levels in Gingival Cervical Fluid from Chronic Periodontitis Patients.
Lavu V, Venkatesan V, Venugopal P, Lakkakula BV, Paul SF, Peria K, Rao SR
Iranian journal of immunology : IJI14(1)51-58(2017 Mar)

Tumor necrosis factor suppresses interleukin 10 in peripheral B cells via upregulating Bcl2-like protein 12 in patients with inflammatory bowel disease.
Guo X, Li MG, Li SS, Liu FH, Liu ZJ, Yang PC
Cell biochemistry and function35(2)77-82(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Interleukin 1B proinflammatory genotypes protect against gastro-oesophageal reflux disease through induction of corpus atrophy.

By using murine fibrosarcoma L929 cells, in which tumor necrosis factor (TNF) alpha induces accumulation of reactive oxygen species (ROS) and cell death, study shows that TNFalpha induces the unfolded protein response in a ROS-dependent fashion
Title: Tumor necrosis factor alpha (TNFalpha) induces the unfolded protein response (UPR) in a reactive oxygen species (ROS)-dependent fashion, and the UPR counteracts ROS accumulation by TNFalpha.

Observational study of gene-disease association. (HuGE Navigator)
Title: Association of polymorphism of tumor necrosis factor-alpha gene promoter region with outcome of hepatitis B virus infection.

Observational study of genotype prevalence. (HuGE Navigator)
Title: Cytokine gene polymorphisms in population of ethnic Macedonians.

During tuberculosis, predisposition of CD4 T-cell subsets to apoptosis may involve both low expression of Bcl-2 and excessive expression of tumor necrosis factor(TNF)-alpha.
Title: Mechanisms of apoptosis of T-cells in human tuberculosis.

The following TNF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OHu27508 NM_000594.3
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Homo sapiens tumor necrosis factor (TNF), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27508
Clone ID Related Accession (Same CDS sequence) NM_000594.3
Accession Version NM_000594.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 702bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product tumor necrosis factor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP215875.1, BC028148.1 and CA306559.1. This sequence is a reference standard in the RefSeqGene project. On Jul 12, 2012 this sequence version replaced NM_000594.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.559704.1, BC028148.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGAGCACTG AAAGCATGAT CCGGGACGTG GAGCTGGCCG AGGAGGCGCT CCCCAAGAAG 
ACAGGGGGGC CCCAGGGCTC CAGGCGGTGC TTGTTCCTCA GCCTCTTCTC CTTCCTGATC
GTGGCAGGCG CCACCACGCT CTTCTGCCTG CTGCACTTTG GAGTGATCGG CCCCCAGAGG
GAAGAGTTCC CCAGGGACCT CTCTCTAATC AGCCCTCTGG CCCAGGCAGT CAGATCATCT
TCTCGAACCC CGAGTGACAA GCCTGTAGCC CATGTTGTAG CAAACCCTCA AGCTGAGGGG
CAGCTCCAGT GGCTGAACCG CCGGGCCAAT GCCCTCCTGG CCAATGGCGT GGAGCTGAGA
GATAACCAGC TGGTGGTGCC ATCAGAGGGC CTGTACCTCA TCTACTCCCA GGTCCTCTTC
AAGGGCCAAG GCTGCCCCTC CACCCATGTG CTCCTCACCC ACACCATCAG CCGCATCGCC
GTCTCCTACC AGACCAAGGT CAACCTCCTC TCTGCCATCA AGAGCCCCTG CCAGAGGGAG
ACCCCAGAGG GGGCTGAGGC CAAGCCCTGG TATGAGCCCA TCTATCTGGG AGGGGTCTTC
CAGCTGGAGA AGGGTGACCG ACTCAGCGCT GAGATCAATC GGCCCGACTA TCTCGACTTT
GCCGAGTCTG GGCAGGTCTA CTTTGGGATC ATTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000585.2
CDS176..877
Misc Feature(1)98..100
Misc Feature(2)179..181
Misc Feature(3)179..181
Misc Feature(4)230..232
Misc Feature(5)233..235
Misc Feature(6)278..283
Misc Feature(7)281..343
Misc Feature(8)290..295
Misc Feature(9)320..325
Misc Feature(10)326..331
Misc Feature(11)395..397
Misc Feature(12)395..397
Misc Feature(13)395..397
Misc Feature(14)401..406
Misc Feature(15)401..403
Misc Feature(16)401..403
Misc Feature(17)410..412
Misc Feature(18)order(446..448,572..574,578..580,758..760,773..775,854..856,866..868)
Misc Feature(19)479..874
Misc Feature(20)order(488..493,506..508,632..634,653..655,668..670)
Exon (1)1..361
Exon (2)362..407
Exon (3)408..455
Exon (4)456..1676
Translation

Target ORF information:

RefSeq Version NM_000594.3
Organism Homo sapiens(Human)
Definition Homo sapiens tumor necrosis factor (TNF), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000594.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGAGCACTG AAAGCATGAT CCGGGACGTG GAGCTGGCCG AGGAGGCGCT CCCCAAGAAG 
ACAGGGGGGC CCCAGGGCTC CAGGCGGTGC TTGTTCCTCA GCCTCTTCTC CTTCCTGATC
GTGGCAGGCG CCACCACGCT CTTCTGCCTG CTGCACTTTG GAGTGATCGG CCCCCAGAGG
GAAGAGTTCC CCAGGGACCT CTCTCTAATC AGCCCTCTGG CCCAGGCAGT CAGATCATCT
TCTCGAACCC CGAGTGACAA GCCTGTAGCC CATGTTGTAG CAAACCCTCA AGCTGAGGGG
CAGCTCCAGT GGCTGAACCG CCGGGCCAAT GCCCTCCTGG CCAATGGCGT GGAGCTGAGA
GATAACCAGC TGGTGGTGCC ATCAGAGGGC CTGTACCTCA TCTACTCCCA GGTCCTCTTC
AAGGGCCAAG GCTGCCCCTC CACCCATGTG CTCCTCACCC ACACCATCAG CCGCATCGCC
GTCTCCTACC AGACCAAGGT CAACCTCCTC TCTGCCATCA AGAGCCCCTG CCAGAGGGAG
ACCCCAGAGG GGGCTGAGGC CAAGCCCTGG TATGAGCCCA TCTATCTGGG AGGGGTCTTC
CAGCTGGAGA AGGGTGACCG ACTCAGCGCT GAGATCAATC GGCCCGACTA TCTCGACTTT
GCCGAGTCTG GGCAGGTCTA CTTTGGGATC ATTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Impaired NK cell functionality and increased TNF-α production as biomarkers of chronic chikungunya arthritis and rheumatoid arthritis.
Human immunology78(4)370-374(2017 Apr)
Thanapati S,Ganu M,Giri P,Kulkarni S,Sharma M,Babar P,Ganu A,Tripathy AS


book

IL-21 Is Positively Associated with Intervertebral Disc Degeneration by Interaction with TNF-α Through the JAK-STAT Signaling Pathway.
Inflammation40(2)612-622(2017 Apr)
Chen B,Liu Y,Zhang Y,Li J,Cheng K,Cheng L


book

Tumor necrosis factor-alpha gene polymorphisms and susceptibility to ischemic heart disease: A systematic review and meta-analysis.
Medicine96(14)e6569(2017 Apr)
Zhang P,Wu X,Li G,He Q,Dai H,Ai C,Shi J


book

Clinical Relevance of Cytokines Gene Polymorphisms and Protein Levels in Gingival Cervical Fluid from Chronic Periodontitis Patients.
ian journal of immunology : IJI14(1)51-58(2017 Mar)
Lavu V,Venkatesan V,Venugopal P,Lakkakula BV,Paul SF,Peria K,Rao SR


book

Tumor necrosis factor suppresses interleukin 10 in peripheral B cells via upregulating Bcl2-like protein 12 in patients with inflammatory bowel disease.
Cell biochemistry and function35(2)77-82(2017 Mar)
Guo X,Li MG,Li SS,Liu FH,Liu ZJ,Yang PC


 
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