CAPN1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CAPN1
Entrez Gene ID	823
Full Name	calpain 1
Synonyms	CANP,CANP1,CANPL1,SPG76,muCANP,muCL
General protein information	Preferred Names calpain 1 Names calpain-1 catalytic subunit CANP 1 calcium-activated neutral proteinase 1 calpain 1, (mu/I) large subunit calpain mu-type calpain, large polypeptide L1 calpain-1 large subunit cell proliferation-inducing gene 30 protein cell proliferation-inducing protein 30 micromolar-calpain
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.1
Summary	The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010].
Disorder MIM:	114220
Disorder Html:	Spastic paraplegia 76, autosomal recessive, 616907 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011545292.1	XP_011543594.1	calpain-1 catalytic subunit isoform X1
XM_006718698.2	XP_006718761.1	calpain-1 catalytic subunit isoform X1
NM_001198869.1	NP_001185798.1	calpain-1 catalytic subunit
NM_001198868.1	NP_001185797.1	calpain-1 catalytic subunit
NM_005186.4	NP_005177.2	calpain-1 catalytic subunit
NM_001198868.2	NP_001185797.1	calpain-1 catalytic subunit
NM_005186.3	NP_005177.2	calpain-1 catalytic subunit
XM_006718698.1	XP_006718761.1	calpain-1 catalytic subunit isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CAPN1	XP_001169100.1
Mus musculus (house mouse)	Capn1	NP_001103974.1
Bos taurus (cattle)	CAPN1	NP_776684.1
Danio rerio (zebrafish)	si:dkeyp-50d11.2	XP_689397.5
Xenopus tropicalis (tropical clawed frog)	capn1	NP_001013632.1
Gallus gallus (chicken)	CAPN1	NP_001038137.1
Danio rerio (zebrafish)	zgc:55262	NP_998624.1
Homo sapiens (human)	CAPN1	NP_001185797.1
	CAPN1	XP_001114172.1
Canis lupus familiaris (dog)	CAPN1	XP_540866.2
Rattus norvegicus (Norway rat)	Capn1	NP_062025.1

Gene Ontology

The following CAPN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAPN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16773	NM_001198868.2 Latest version!	Homo sapiens calpain 1 (CAPN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu16773	NM_001198869.1 Latest version!	Homo sapiens calpain 1 (CAPN1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu16773	NM_005186.4 Latest version!	Homo sapiens calpain 1 (CAPN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu16773	XM_006718698.2 Latest version!	Homo sapiens calpain 1 (CAPN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu16773	XM_011545292.1 Latest version!	Homo sapiens calpain 1 (CAPN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu16773	NM_001198868.1	Homo sapiens calpain 1 (CAPN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu16773	NM_005186.3	Homo sapiens calpain 1 (CAPN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu16773	XM_006718698.1	Homo sapiens calpain 1 (CAPN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CAPN1 ( NM_001198869.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185798.1 Homo sapiens calpain 1 (CAPN1), transcript variant 3, mRNA.

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CloneID	OHu16773
Clone ID Related Accession (Same CDS sequence)	XM_011545292.1 , XM_006718698.2 , NM_001198869.1 , NM_001198868.1 , NM_005186.4 , NM_001198868.2 , NM_005186.3 , XM_006718698.1
Accession Version	NM_001198869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	calpain-1 catalytic subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC372552.1, BC075862.1, AK301538.1 and BC008751.2. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC075862.1, SRR1803611.216394.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAGG AGATCATCAC GCCGGTGTAC TGCACTGGGG TGTCAGCCCA AGTGCAGAAG 
CAGCGGGCCA GGGAGCTGGG CCTGGGCCGC CATGAGAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAGC AGCTGCGGGT GCGATGCCTG CAGAGTGGGA CCCTCTTCCG TGATGAGGCC 
TTCCCCCCGG TACCCCAGAG CCTGGGTTAC AAGGACCTGG GTCCCAATTC CTCCAAGACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAA CTGCTGTCAA ACCCCCAGTT CATTGTGGAT 
GGAGCTACCC GCACAGACAT CTGCCAGGGA GCACTGGGGG ACTGCTGGCT CTTGGCGGCC 
ATCGCCTCCC TCACTCTCAA CGACACCCTC CTGCACCGAG TGGTTCCGCA CGGCCAGAGC 
TTCCAGAATG GCTATGCCGG CATCTTCCAT TTCCAGCTGT GGCAATTTGG GGAGTGGGTG 
GACGTGGTCG TGGATGACCT GCTGCCCATC AAGGACGGGA AGCTAGTGTT CGTGCACTCT 
GCCGAAGGCA ACGAGTTCTG GAGCGCCCTG CTTGAGAAGG CCTATGCCAA GGTAAATGGC 
AGCTACGAGG CCCTGTCAGG GGGCAGCACC TCAGAGGGCT TTGAGGACTT CACAGGCGGG 
GTTACCGAGT GGTACGAGTT GCGCAAGGCT CCCAGTGACC TCTACCAGAT CATCCTCAAG 
GCGCTGGAGC GGGGCTCCCT GCTGGGCTGC TCCATAGACA TCTCCAGCGT TCTAGACATG 
GAGGCCATCA CTTTCAAGAA GTTGGTGAAG GGCCATGCCT ACTCTGTGAC CGGGGCCAAG 
CAGGTGAACT ACCGAGGCCA GGTGGTGAGC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CGGGAGCCTG GAGCGACAGC TCCTCAGAGT GGAACAACGT GGACCCATAT 
GAACGGGACC AGCTCCGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC ATTCCGAGAC 
TTCATGCGGG AGTTCACCCG CCTGGAGATC TGCAACCTCA CACCCGACGC CCTCAAGAGC 
CGGACCATCC GCAAATGGAA CACCACACTC TACGAAGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGAAACTA CCCAGCCACC TTCTGGGTGA ACCCTCAGTT CAAGATCCGG 
CTGGATGAGA CGGATGACCC GGACGACTAC GGGGACCGCG AGTCAGGCTG CAGCTTCGTG 
CTCGCCCTTA TGCAGAAGCA CCGTCGCCGC GAGCGCCGCT TCGGCCGCGA CATGGAGACT 
ATTGGCTTCG CGGTCTACGA GGTCCCTCCG GAGCTGGTGG GCCAGCCGGC CGTACACTTG 
AAGCGTGACT TCTTCCTGGC CAATGCGTCT CGGGCGCGCT CAGAGCAGTT CATCAACCTG 
CGAGAGGTCA GCACCCGCTT CCGCCTGCCA CCCGGGGAGT ATGTGGTGGT GCCCTCCACC 
TTCGAGCCCA ACAAGGAGGG CGACTTCGTG CTGCGCTTCT TCTCAGAGAA GAGTGCTGGG 
ACTGTGGAGC TGGATGACCA GATCCAGGCC AATCTCCCCG ATGAGCAAGT GCTCTCAGAA 
GAGGAGATTG ACGAGAACTT CAAGGCCCTC TTCAGGCAGC TGGCAGGGGA GGACATGGAG 
ATCAGCGTGA AGGAGTTGCG GACAATCCTC AATAGGATCA TCAGCAAACA CAAAGACCTG 
CGGACCAAGG GCTTCAGCCT AGAGTCGTGC CGCAGCATGG TGAACCTCAT GGATCGTGAT 
GGCAATGGGA AGCTGGGCCT GGTGGAGTTC AACATCCTGT GGAACCGCAT CCGGAATTAC 
CTGTCCATCT TCCGGAAGTT TGACCTGGAC AAGTCGGGCA GCATGAGTGC CTACGAGATG 
CGGATGGCCA TTGAGTCGGC AGGCTTCAAG CTCAACAAGA AGCTGTACGA GCTCATCATC 
ACCCGCTACT CGGAGCCCGA CCTGGCGGTC GACTTTGACA ATTTCGTTTG CTGCCTGGTG 
CGGCTAGAGA CCATGTTCCG ATTTTTCAAA ACTCTGGACA CAGATCTGGA TGGAGTTGTG 
ACCTTTGACT TGTTTAAGTG GTTGCAGCTG ACCATGTTTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185798.1
CDS	167..2311
Translation	MSEEIITPVYCTGVSAQVQKQRARELGLGRHENAIKYLGQDYEQLRVRCLQSGTLFRDEAFPPVPQSLGYKDLGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDLYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQVNYRGQVVSLIRMRNPWGEVEWTGAWSDSSSEWNNVDPYERDQLRVKMEDGEFWMSFRDFMREFTRLEICNLTPDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPDEQVLSEEEIDENFKALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA

Target ORF information:

RefSeq Version	NM_001198869.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 1 (CAPN1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198869.1

ORF Insert Sequence:

ATGTCGGAGG AGATCATCAC GCCGGTGTAC TGCACTGGGG TGTCAGCCCA AGTGCAGAAG 
CAGCGGGCCA GGGAGCTGGG CCTGGGCCGC CATGAGAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAGC AGCTGCGGGT GCGATGCCTG CAGAGTGGGA CCCTCTTCCG TGATGAGGCC 
TTCCCCCCGG TACCCCAGAG CCTGGGTTAC AAGGACCTGG GTCCCAATTC CTCCAAGACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAA CTGCTGTCAA ACCCCCAGTT CATTGTGGAT 
GGAGCTACCC GCACAGACAT CTGCCAGGGA GCACTGGGGG ACTGCTGGCT CTTGGCGGCC 
ATCGCCTCCC TCACTCTCAA CGACACCCTC CTGCACCGAG TGGTTCCGCA CGGCCAGAGC 
TTCCAGAATG GCTATGCCGG CATCTTCCAT TTCCAGCTGT GGCAATTTGG GGAGTGGGTG 
GACGTGGTCG TGGATGACCT GCTGCCCATC AAGGACGGGA AGCTAGTGTT CGTGCACTCT 
GCCGAAGGCA ACGAGTTCTG GAGCGCCCTG CTTGAGAAGG CCTATGCCAA GGTAAATGGC 
AGCTACGAGG CCCTGTCAGG GGGCAGCACC TCAGAGGGCT TTGAGGACTT CACAGGCGGG 
GTTACCGAGT GGTACGAGTT GCGCAAGGCT CCCAGTGACC TCTACCAGAT CATCCTCAAG 
GCGCTGGAGC GGGGCTCCCT GCTGGGCTGC TCCATAGACA TCTCCAGCGT TCTAGACATG 
GAGGCCATCA CTTTCAAGAA GTTGGTGAAG GGCCATGCCT ACTCTGTGAC CGGGGCCAAG 
CAGGTGAACT ACCGAGGCCA GGTGGTGAGC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CGGGAGCCTG GAGCGACAGC TCCTCAGAGT GGAACAACGT GGACCCATAT 
GAACGGGACC AGCTCCGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC ATTCCGAGAC 
TTCATGCGGG AGTTCACCCG CCTGGAGATC TGCAACCTCA CACCCGACGC CCTCAAGAGC 
CGGACCATCC GCAAATGGAA CACCACACTC TACGAAGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGAAACTA CCCAGCCACC TTCTGGGTGA ACCCTCAGTT CAAGATCCGG 
CTGGATGAGA CGGATGACCC GGACGACTAC GGGGACCGCG AGTCAGGCTG CAGCTTCGTG 
CTCGCCCTTA TGCAGAAGCA CCGTCGCCGC GAGCGCCGCT TCGGCCGCGA CATGGAGACT 
ATTGGCTTCG CGGTCTACGA GGTCCCTCCG GAGCTGGTGG GCCAGCCGGC CGTACACTTG 
AAGCGTGACT TCTTCCTGGC CAATGCGTCT CGGGCGCGCT CAGAGCAGTT CATCAACCTG 
CGAGAGGTCA GCACCCGCTT CCGCCTGCCA CCCGGGGAGT ATGTGGTGGT GCCCTCCACC 
TTCGAGCCCA ACAAGGAGGG CGACTTCGTG CTGCGCTTCT TCTCAGAGAA GAGTGCTGGG 
ACTGTGGAGC TGGATGACCA GATCCAGGCC AATCTCCCCG ATGAGCAAGT GCTCTCAGAA 
GAGGAGATTG ACGAGAACTT CAAGGCCCTC TTCAGGCAGC TGGCAGGGGA GGACATGGAG 
ATCAGCGTGA AGGAGTTGCG GACAATCCTC AATAGGATCA TCAGCAAACA CAAAGACCTG 
CGGACCAAGG GCTTCAGCCT AGAGTCGTGC CGCAGCATGG TGAACCTCAT GGATCGTGAT 
GGCAATGGGA AGCTGGGCCT GGTGGAGTTC AACATCCTGT GGAACCGCAT CCGGAATTAC 
CTGTCCATCT TCCGGAAGTT TGACCTGGAC AAGTCGGGCA GCATGAGTGC CTACGAGATG 
CGGATGGCCA TTGAGTCGGC AGGCTTCAAG CTCAACAAGA AGCTGTACGA GCTCATCATC 
ACCCGCTACT CGGAGCCCGA CCTGGCGGTC GACTTTGACA ATTTCGTTTG CTGCCTGGTG 
CGGCTAGAGA CCATGTTCCG ATTTTTCAAA ACTCTGGACA CAGATCTGGA TGGAGTTGTG 
ACCTTTGACT TGTTTAAGTG GTTGCAGCTG ACCATGTTTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN1 ( NM_001198868.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185797.1 Homo sapiens calpain 1 (CAPN1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16773
Clone ID Related Accession (Same CDS sequence)	XM_011545292.1 , XM_006718698.2 , NM_001198869.1 , NM_001198868.1 , NM_005186.4 , NM_001198868.2 , NM_005186.3 , XM_006718698.1
Accession Version	NM_001198868.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	calpain-1 catalytic subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA788342.1, AK315556.1, BC008751.2 and X04366.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2, and 3 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.31177.1, SRR1803614.170799.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAGG AGATCATCAC GCCGGTGTAC TGCACTGGGG TGTCAGCCCA AGTGCAGAAG 
CAGCGGGCCA GGGAGCTGGG CCTGGGCCGC CATGAGAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAGC AGCTGCGGGT GCGATGCCTG CAGAGTGGGA CCCTCTTCCG TGATGAGGCC 
TTCCCCCCGG TACCCCAGAG CCTGGGTTAC AAGGACCTGG GTCCCAATTC CTCCAAGACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAA CTGCTGTCAA ACCCCCAGTT CATTGTGGAT 
GGAGCTACCC GCACAGACAT CTGCCAGGGA GCACTGGGGG ACTGCTGGCT CTTGGCGGCC 
ATCGCCTCCC TCACTCTCAA CGACACCCTC CTGCACCGAG TGGTTCCGCA CGGCCAGAGC 
TTCCAGAATG GCTATGCCGG CATCTTCCAT TTCCAGCTGT GGCAATTTGG GGAGTGGGTG 
GACGTGGTCG TGGATGACCT GCTGCCCATC AAGGACGGGA AGCTAGTGTT CGTGCACTCT 
GCCGAAGGCA ACGAGTTCTG GAGCGCCCTG CTTGAGAAGG CCTATGCCAA GGTAAATGGC 
AGCTACGAGG CCCTGTCAGG GGGCAGCACC TCAGAGGGCT TTGAGGACTT CACAGGCGGG 
GTTACCGAGT GGTACGAGTT GCGCAAGGCT CCCAGTGACC TCTACCAGAT CATCCTCAAG 
GCGCTGGAGC GGGGCTCCCT GCTGGGCTGC TCCATAGACA TCTCCAGCGT TCTAGACATG 
GAGGCCATCA CTTTCAAGAA GTTGGTGAAG GGCCATGCCT ACTCTGTGAC CGGGGCCAAG 
CAGGTGAACT ACCGAGGCCA GGTGGTGAGC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CGGGAGCCTG GAGCGACAGC TCCTCAGAGT GGAACAACGT GGACCCATAT 
GAACGGGACC AGCTCCGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC ATTCCGAGAC 
TTCATGCGGG AGTTCACCCG CCTGGAGATC TGCAACCTCA CACCCGACGC CCTCAAGAGC 
CGGACCATCC GCAAATGGAA CACCACACTC TACGAAGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGAAACTA CCCAGCCACC TTCTGGGTGA ACCCTCAGTT CAAGATCCGG 
CTGGATGAGA CGGATGACCC GGACGACTAC GGGGACCGCG AGTCAGGCTG CAGCTTCGTG 
CTCGCCCTTA TGCAGAAGCA CCGTCGCCGC GAGCGCCGCT TCGGCCGCGA CATGGAGACT 
ATTGGCTTCG CGGTCTACGA GGTCCCTCCG GAGCTGGTGG GCCAGCCGGC CGTACACTTG 
AAGCGTGACT TCTTCCTGGC CAATGCGTCT CGGGCGCGCT CAGAGCAGTT CATCAACCTG 
CGAGAGGTCA GCACCCGCTT CCGCCTGCCA CCCGGGGAGT ATGTGGTGGT GCCCTCCACC 
TTCGAGCCCA ACAAGGAGGG CGACTTCGTG CTGCGCTTCT TCTCAGAGAA GAGTGCTGGG 
ACTGTGGAGC TGGATGACCA GATCCAGGCC AATCTCCCCG ATGAGCAAGT GCTCTCAGAA 
GAGGAGATTG ACGAGAACTT CAAGGCCCTC TTCAGGCAGC TGGCAGGGGA GGACATGGAG 
ATCAGCGTGA AGGAGTTGCG GACAATCCTC AATAGGATCA TCAGCAAACA CAAAGACCTG 
CGGACCAAGG GCTTCAGCCT AGAGTCGTGC CGCAGCATGG TGAACCTCAT GGATCGTGAT 
GGCAATGGGA AGCTGGGCCT GGTGGAGTTC AACATCCTGT GGAACCGCAT CCGGAATTAC 
CTGTCCATCT TCCGGAAGTT TGACCTGGAC AAGTCGGGCA GCATGAGTGC CTACGAGATG 
CGGATGGCCA TTGAGTCGGC AGGCTTCAAG CTCAACAAGA AGCTGTACGA GCTCATCATC 
ACCCGCTACT CGGAGCCCGA CCTGGCGGTC GACTTTGACA ATTTCGTTTG CTGCCTGGTG 
CGGCTAGAGA CCATGTTCCG ATTTTTCAAA ACTCTGGACA CAGATCTGGA TGGAGTTGTG 
ACCTTTGACT TGTTTAAGTG GTTGCAGCTG ACCATGTTTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185797.1
CDS	198..2342
Translation	MSEEIITPVYCTGVSAQVQKQRARELGLGRHENAIKYLGQDYEQLRVRCLQSGTLFRDEAFPPVPQSLGYKDLGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDLYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQVNYRGQVVSLIRMRNPWGEVEWTGAWSDSSSEWNNVDPYERDQLRVKMEDGEFWMSFRDFMREFTRLEICNLTPDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPDEQVLSEEEIDENFKALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA

Target ORF information:

RefSeq Version	NM_001198868.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 1 (CAPN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198868.1

ORF Insert Sequence:

ATGTCGGAGG AGATCATCAC GCCGGTGTAC TGCACTGGGG TGTCAGCCCA AGTGCAGAAG 
CAGCGGGCCA GGGAGCTGGG CCTGGGCCGC CATGAGAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAGC AGCTGCGGGT GCGATGCCTG CAGAGTGGGA CCCTCTTCCG TGATGAGGCC 
TTCCCCCCGG TACCCCAGAG CCTGGGTTAC AAGGACCTGG GTCCCAATTC CTCCAAGACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAA CTGCTGTCAA ACCCCCAGTT CATTGTGGAT 
GGAGCTACCC GCACAGACAT CTGCCAGGGA GCACTGGGGG ACTGCTGGCT CTTGGCGGCC 
ATCGCCTCCC TCACTCTCAA CGACACCCTC CTGCACCGAG TGGTTCCGCA CGGCCAGAGC 
TTCCAGAATG GCTATGCCGG CATCTTCCAT TTCCAGCTGT GGCAATTTGG GGAGTGGGTG 
GACGTGGTCG TGGATGACCT GCTGCCCATC AAGGACGGGA AGCTAGTGTT CGTGCACTCT 
GCCGAAGGCA ACGAGTTCTG GAGCGCCCTG CTTGAGAAGG CCTATGCCAA GGTAAATGGC 
AGCTACGAGG CCCTGTCAGG GGGCAGCACC TCAGAGGGCT TTGAGGACTT CACAGGCGGG 
GTTACCGAGT GGTACGAGTT GCGCAAGGCT CCCAGTGACC TCTACCAGAT CATCCTCAAG 
GCGCTGGAGC GGGGCTCCCT GCTGGGCTGC TCCATAGACA TCTCCAGCGT TCTAGACATG 
GAGGCCATCA CTTTCAAGAA GTTGGTGAAG GGCCATGCCT ACTCTGTGAC CGGGGCCAAG 
CAGGTGAACT ACCGAGGCCA GGTGGTGAGC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CGGGAGCCTG GAGCGACAGC TCCTCAGAGT GGAACAACGT GGACCCATAT 
GAACGGGACC AGCTCCGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC ATTCCGAGAC 
TTCATGCGGG AGTTCACCCG CCTGGAGATC TGCAACCTCA CACCCGACGC CCTCAAGAGC 
CGGACCATCC GCAAATGGAA CACCACACTC TACGAAGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGAAACTA CCCAGCCACC TTCTGGGTGA ACCCTCAGTT CAAGATCCGG 
CTGGATGAGA CGGATGACCC GGACGACTAC GGGGACCGCG AGTCAGGCTG CAGCTTCGTG 
CTCGCCCTTA TGCAGAAGCA CCGTCGCCGC GAGCGCCGCT TCGGCCGCGA CATGGAGACT 
ATTGGCTTCG CGGTCTACGA GGTCCCTCCG GAGCTGGTGG GCCAGCCGGC CGTACACTTG 
AAGCGTGACT TCTTCCTGGC CAATGCGTCT CGGGCGCGCT CAGAGCAGTT CATCAACCTG 
CGAGAGGTCA GCACCCGCTT CCGCCTGCCA CCCGGGGAGT ATGTGGTGGT GCCCTCCACC 
TTCGAGCCCA ACAAGGAGGG CGACTTCGTG CTGCGCTTCT TCTCAGAGAA GAGTGCTGGG 
ACTGTGGAGC TGGATGACCA GATCCAGGCC AATCTCCCCG ATGAGCAAGT GCTCTCAGAA 
GAGGAGATTG ACGAGAACTT CAAGGCCCTC TTCAGGCAGC TGGCAGGGGA GGACATGGAG 
ATCAGCGTGA AGGAGTTGCG GACAATCCTC AATAGGATCA TCAGCAAACA CAAAGACCTG 
CGGACCAAGG GCTTCAGCCT AGAGTCGTGC CGCAGCATGG TGAACCTCAT GGATCGTGAT 
GGCAATGGGA AGCTGGGCCT GGTGGAGTTC AACATCCTGT GGAACCGCAT CCGGAATTAC 
CTGTCCATCT TCCGGAAGTT TGACCTGGAC AAGTCGGGCA GCATGAGTGC CTACGAGATG 
CGGATGGCCA TTGAGTCGGC AGGCTTCAAG CTCAACAAGA AGCTGTACGA GCTCATCATC 
ACCCGCTACT CGGAGCCCGA CCTGGCGGTC GACTTTGACA ATTTCGTTTG CTGCCTGGTG 
CGGCTAGAGA CCATGTTCCG ATTTTTCAAA ACTCTGGACA CAGATCTGGA TGGAGTTGTG 
ACCTTTGACT TGTTTAAGTG GTTGCAGCTG ACCATGTTTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN1 ( NM_005186.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005177.2 Homo sapiens calpain 1 (CAPN1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16773
Clone ID Related Accession (Same CDS sequence)	XM_011545292.1 , XM_006718698.2 , NM_001198869.1 , NM_001198868.1 , NM_005186.4 , NM_001198868.2 , NM_005186.3 , XM_006718698.1
Accession Version	NM_005186.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	calpain-1 catalytic subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC008751.2 and X04366.1. On Jun 2, 2019 this sequence version replaced NM_005186.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.53159.1, BC008751.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAGG AGATCATCAC GCCGGTGTAC TGCACTGGGG TGTCAGCCCA AGTGCAGAAG 
CAGCGGGCCA GGGAGCTGGG CCTGGGCCGC CATGAGAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAGC AGCTGCGGGT GCGATGCCTG CAGAGTGGGA CCCTCTTCCG TGATGAGGCC 
TTCCCCCCGG TACCCCAGAG CCTGGGTTAC AAGGACCTGG GTCCCAATTC CTCCAAGACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAA CTGCTGTCAA ACCCCCAGTT CATTGTGGAT 
GGAGCTACCC GCACAGACAT CTGCCAGGGA GCACTGGGGG ACTGCTGGCT CTTGGCGGCC 
ATCGCCTCCC TCACTCTCAA CGACACCCTC CTGCACCGAG TGGTTCCGCA CGGCCAGAGC 
TTCCAGAATG GCTATGCCGG CATCTTCCAT TTCCAGCTGT GGCAATTTGG GGAGTGGGTG 
GACGTGGTCG TGGATGACCT GCTGCCCATC AAGGACGGGA AGCTAGTGTT CGTGCACTCT 
GCCGAAGGCA ACGAGTTCTG GAGCGCCCTG CTTGAGAAGG CCTATGCCAA GGTAAATGGC 
AGCTACGAGG CCCTGTCAGG GGGCAGCACC TCAGAGGGCT TTGAGGACTT CACAGGCGGG 
GTTACCGAGT GGTACGAGTT GCGCAAGGCT CCCAGTGACC TCTACCAGAT CATCCTCAAG 
GCGCTGGAGC GGGGCTCCCT GCTGGGCTGC TCCATAGACA TCTCCAGCGT TCTAGACATG 
GAGGCCATCA CTTTCAAGAA GTTGGTGAAG GGCCATGCCT ACTCTGTGAC CGGGGCCAAG 
CAGGTGAACT ACCGAGGCCA GGTGGTGAGC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CGGGAGCCTG GAGCGACAGC TCCTCAGAGT GGAACAACGT GGACCCATAT 
GAACGGGACC AGCTCCGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC ATTCCGAGAC 
TTCATGCGGG AGTTCACCCG CCTGGAGATC TGCAACCTCA CACCCGACGC CCTCAAGAGC 
CGGACCATCC GCAAATGGAA CACCACACTC TACGAAGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGAAACTA CCCAGCCACC TTCTGGGTGA ACCCTCAGTT CAAGATCCGG 
CTGGATGAGA CGGATGACCC GGACGACTAC GGGGACCGCG AGTCAGGCTG CAGCTTCGTG 
CTCGCCCTTA TGCAGAAGCA CCGTCGCCGC GAGCGCCGCT TCGGCCGCGA CATGGAGACT 
ATTGGCTTCG CGGTCTACGA GGTCCCTCCG GAGCTGGTGG GCCAGCCGGC CGTACACTTG 
AAGCGTGACT TCTTCCTGGC CAATGCGTCT CGGGCGCGCT CAGAGCAGTT CATCAACCTG 
CGAGAGGTCA GCACCCGCTT CCGCCTGCCA CCCGGGGAGT ATGTGGTGGT GCCCTCCACC 
TTCGAGCCCA ACAAGGAGGG CGACTTCGTG CTGCGCTTCT TCTCAGAGAA GAGTGCTGGG 
ACTGTGGAGC TGGATGACCA GATCCAGGCC AATCTCCCCG ATGAGCAAGT GCTCTCAGAA 
GAGGAGATTG ACGAGAACTT CAAGGCCCTC TTCAGGCAGC TGGCAGGGGA GGACATGGAG 
ATCAGCGTGA AGGAGTTGCG GACAATCCTC AATAGGATCA TCAGCAAACA CAAAGACCTG 
CGGACCAAGG GCTTCAGCCT AGAGTCGTGC CGCAGCATGG TGAACCTCAT GGATCGTGAT 
GGCAATGGGA AGCTGGGCCT GGTGGAGTTC AACATCCTGT GGAACCGCAT CCGGAATTAC 
CTGTCCATCT TCCGGAAGTT TGACCTGGAC AAGTCGGGCA GCATGAGTGC CTACGAGATG 
CGGATGGCCA TTGAGTCGGC AGGCTTCAAG CTCAACAAGA AGCTGTACGA GCTCATCATC 
ACCCGCTACT CGGAGCCCGA CCTGGCGGTC GACTTTGACA ATTTCGTTTG CTGCCTGGTG 
CGGCTAGAGA CCATGTTCCG ATTTTTCAAA ACTCTGGACA CAGATCTGGA TGGAGTTGTG 
ACCTTTGACT TGTTTAAGTG GTTGCAGCTG ACCATGTTTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005177.2
CDS	88..2232
Translation	MSEEIITPVYCTGVSAQVQKQRARELGLGRHENAIKYLGQDYEQLRVRCLQSGTLFRDEAFPPVPQSLGYKDLGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDLYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQVNYRGQVVSLIRMRNPWGEVEWTGAWSDSSSEWNNVDPYERDQLRVKMEDGEFWMSFRDFMREFTRLEICNLTPDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPDEQVLSEEEIDENFKALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA

Target ORF information:

RefSeq Version	NM_005186.4
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 1 (CAPN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005186.4

ORF Insert Sequence:

ATGTCGGAGG AGATCATCAC GCCGGTGTAC TGCACTGGGG TGTCAGCCCA AGTGCAGAAG 
CAGCGGGCCA GGGAGCTGGG CCTGGGCCGC CATGAGAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAGC AGCTGCGGGT GCGATGCCTG CAGAGTGGGA CCCTCTTCCG TGATGAGGCC 
TTCCCCCCGG TACCCCAGAG CCTGGGTTAC AAGGACCTGG GTCCCAATTC CTCCAAGACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAA CTGCTGTCAA ACCCCCAGTT CATTGTGGAT 
GGAGCTACCC GCACAGACAT CTGCCAGGGA GCACTGGGGG ACTGCTGGCT CTTGGCGGCC 
ATCGCCTCCC TCACTCTCAA CGACACCCTC CTGCACCGAG TGGTTCCGCA CGGCCAGAGC 
TTCCAGAATG GCTATGCCGG CATCTTCCAT TTCCAGCTGT GGCAATTTGG GGAGTGGGTG 
GACGTGGTCG TGGATGACCT GCTGCCCATC AAGGACGGGA AGCTAGTGTT CGTGCACTCT 
GCCGAAGGCA ACGAGTTCTG GAGCGCCCTG CTTGAGAAGG CCTATGCCAA GGTAAATGGC 
AGCTACGAGG CCCTGTCAGG GGGCAGCACC TCAGAGGGCT TTGAGGACTT CACAGGCGGG 
GTTACCGAGT GGTACGAGTT GCGCAAGGCT CCCAGTGACC TCTACCAGAT CATCCTCAAG 
GCGCTGGAGC GGGGCTCCCT GCTGGGCTGC TCCATAGACA TCTCCAGCGT TCTAGACATG 
GAGGCCATCA CTTTCAAGAA GTTGGTGAAG GGCCATGCCT ACTCTGTGAC CGGGGCCAAG 
CAGGTGAACT ACCGAGGCCA GGTGGTGAGC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CGGGAGCCTG GAGCGACAGC TCCTCAGAGT GGAACAACGT GGACCCATAT 
GAACGGGACC AGCTCCGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC ATTCCGAGAC 
TTCATGCGGG AGTTCACCCG CCTGGAGATC TGCAACCTCA CACCCGACGC CCTCAAGAGC 
CGGACCATCC GCAAATGGAA CACCACACTC TACGAAGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGAAACTA CCCAGCCACC TTCTGGGTGA ACCCTCAGTT CAAGATCCGG 
CTGGATGAGA CGGATGACCC GGACGACTAC GGGGACCGCG AGTCAGGCTG CAGCTTCGTG 
CTCGCCCTTA TGCAGAAGCA CCGTCGCCGC GAGCGCCGCT TCGGCCGCGA CATGGAGACT 
ATTGGCTTCG CGGTCTACGA GGTCCCTCCG GAGCTGGTGG GCCAGCCGGC CGTACACTTG 
AAGCGTGACT TCTTCCTGGC CAATGCGTCT CGGGCGCGCT CAGAGCAGTT CATCAACCTG 
CGAGAGGTCA GCACCCGCTT CCGCCTGCCA CCCGGGGAGT ATGTGGTGGT GCCCTCCACC 
TTCGAGCCCA ACAAGGAGGG CGACTTCGTG CTGCGCTTCT TCTCAGAGAA GAGTGCTGGG 
ACTGTGGAGC TGGATGACCA GATCCAGGCC AATCTCCCCG ATGAGCAAGT GCTCTCAGAA 
GAGGAGATTG ACGAGAACTT CAAGGCCCTC TTCAGGCAGC TGGCAGGGGA GGACATGGAG 
ATCAGCGTGA AGGAGTTGCG GACAATCCTC AATAGGATCA TCAGCAAACA CAAAGACCTG 
CGGACCAAGG GCTTCAGCCT AGAGTCGTGC CGCAGCATGG TGAACCTCAT GGATCGTGAT 
GGCAATGGGA AGCTGGGCCT GGTGGAGTTC AACATCCTGT GGAACCGCAT CCGGAATTAC 
CTGTCCATCT TCCGGAAGTT TGACCTGGAC AAGTCGGGCA GCATGAGTGC CTACGAGATG 
CGGATGGCCA TTGAGTCGGC AGGCTTCAAG CTCAACAAGA AGCTGTACGA GCTCATCATC 
ACCCGCTACT CGGAGCCCGA CCTGGCGGTC GACTTTGACA ATTTCGTTTG CTGCCTGGTG 
CGGCTAGAGA CCATGTTCCG ATTTTTCAAA ACTCTGGACA CAGATCTGGA TGGAGTTGTG 
ACCTTTGACT TGTTTAAGTG GTTGCAGCTG ACCATGTTTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN1 ( NM_001198868.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185797.1 Homo sapiens calpain 1 (CAPN1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16773
Clone ID Related Accession (Same CDS sequence)	XM_011545292.1 , XM_006718698.2 , NM_001198869.1 , NM_001198868.1 , NM_005186.4 , NM_001198868.2 , NM_005186.3 , XM_006718698.1
Accession Version	NM_001198868.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	calpain-1 catalytic subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315556.1, BC008751.2 and X04366.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001198868.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2, and 3 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.234198.1, SRR1803614.170799.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAGG AGATCATCAC GCCGGTGTAC TGCACTGGGG TGTCAGCCCA AGTGCAGAAG 
CAGCGGGCCA GGGAGCTGGG CCTGGGCCGC CATGAGAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAGC AGCTGCGGGT GCGATGCCTG CAGAGTGGGA CCCTCTTCCG TGATGAGGCC 
TTCCCCCCGG TACCCCAGAG CCTGGGTTAC AAGGACCTGG GTCCCAATTC CTCCAAGACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAA CTGCTGTCAA ACCCCCAGTT CATTGTGGAT 
GGAGCTACCC GCACAGACAT CTGCCAGGGA GCACTGGGGG ACTGCTGGCT CTTGGCGGCC 
ATCGCCTCCC TCACTCTCAA CGACACCCTC CTGCACCGAG TGGTTCCGCA CGGCCAGAGC 
TTCCAGAATG GCTATGCCGG CATCTTCCAT TTCCAGCTGT GGCAATTTGG GGAGTGGGTG 
GACGTGGTCG TGGATGACCT GCTGCCCATC AAGGACGGGA AGCTAGTGTT CGTGCACTCT 
GCCGAAGGCA ACGAGTTCTG GAGCGCCCTG CTTGAGAAGG CCTATGCCAA GGTAAATGGC 
AGCTACGAGG CCCTGTCAGG GGGCAGCACC TCAGAGGGCT TTGAGGACTT CACAGGCGGG 
GTTACCGAGT GGTACGAGTT GCGCAAGGCT CCCAGTGACC TCTACCAGAT CATCCTCAAG 
GCGCTGGAGC GGGGCTCCCT GCTGGGCTGC TCCATAGACA TCTCCAGCGT TCTAGACATG 
GAGGCCATCA CTTTCAAGAA GTTGGTGAAG GGCCATGCCT ACTCTGTGAC CGGGGCCAAG 
CAGGTGAACT ACCGAGGCCA GGTGGTGAGC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CGGGAGCCTG GAGCGACAGC TCCTCAGAGT GGAACAACGT GGACCCATAT 
GAACGGGACC AGCTCCGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC ATTCCGAGAC 
TTCATGCGGG AGTTCACCCG CCTGGAGATC TGCAACCTCA CACCCGACGC CCTCAAGAGC 
CGGACCATCC GCAAATGGAA CACCACACTC TACGAAGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGAAACTA CCCAGCCACC TTCTGGGTGA ACCCTCAGTT CAAGATCCGG 
CTGGATGAGA CGGATGACCC GGACGACTAC GGGGACCGCG AGTCAGGCTG CAGCTTCGTG 
CTCGCCCTTA TGCAGAAGCA CCGTCGCCGC GAGCGCCGCT TCGGCCGCGA CATGGAGACT 
ATTGGCTTCG CGGTCTACGA GGTCCCTCCG GAGCTGGTGG GCCAGCCGGC CGTACACTTG 
AAGCGTGACT TCTTCCTGGC CAATGCGTCT CGGGCGCGCT CAGAGCAGTT CATCAACCTG 
CGAGAGGTCA GCACCCGCTT CCGCCTGCCA CCCGGGGAGT ATGTGGTGGT GCCCTCCACC 
TTCGAGCCCA ACAAGGAGGG CGACTTCGTG CTGCGCTTCT TCTCAGAGAA GAGTGCTGGG 
ACTGTGGAGC TGGATGACCA GATCCAGGCC AATCTCCCCG ATGAGCAAGT GCTCTCAGAA 
GAGGAGATTG ACGAGAACTT CAAGGCCCTC TTCAGGCAGC TGGCAGGGGA GGACATGGAG 
ATCAGCGTGA AGGAGTTGCG GACAATCCTC AATAGGATCA TCAGCAAACA CAAAGACCTG 
CGGACCAAGG GCTTCAGCCT AGAGTCGTGC CGCAGCATGG TGAACCTCAT GGATCGTGAT 
GGCAATGGGA AGCTGGGCCT GGTGGAGTTC AACATCCTGT GGAACCGCAT CCGGAATTAC 
CTGTCCATCT TCCGGAAGTT TGACCTGGAC AAGTCGGGCA GCATGAGTGC CTACGAGATG 
CGGATGGCCA TTGAGTCGGC AGGCTTCAAG CTCAACAAGA AGCTGTACGA GCTCATCATC 
ACCCGCTACT CGGAGCCCGA CCTGGCGGTC GACTTTGACA ATTTCGTTTG CTGCCTGGTG 
CGGCTAGAGA CCATGTTCCG ATTTTTCAAA ACTCTGGACA CAGATCTGGA TGGAGTTGTG 
ACCTTTGACT TGTTTAAGTG GTTGCAGCTG ACCATGTTTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185797.1
CDS	40..2184
Translation	MSEEIITPVYCTGVSAQVQKQRARELGLGRHENAIKYLGQDYEQLRVRCLQSGTLFRDEAFPPVPQSLGYKDLGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDLYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQVNYRGQVVSLIRMRNPWGEVEWTGAWSDSSSEWNNVDPYERDQLRVKMEDGEFWMSFRDFMREFTRLEICNLTPDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPDEQVLSEEEIDENFKALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA

Target ORF information:

RefSeq Version	NM_001198868.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 1 (CAPN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198868.2

ORF Insert Sequence:

ATGTCGGAGG AGATCATCAC GCCGGTGTAC TGCACTGGGG TGTCAGCCCA AGTGCAGAAG 
CAGCGGGCCA GGGAGCTGGG CCTGGGCCGC CATGAGAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAGC AGCTGCGGGT GCGATGCCTG CAGAGTGGGA CCCTCTTCCG TGATGAGGCC 
TTCCCCCCGG TACCCCAGAG CCTGGGTTAC AAGGACCTGG GTCCCAATTC CTCCAAGACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAA CTGCTGTCAA ACCCCCAGTT CATTGTGGAT 
GGAGCTACCC GCACAGACAT CTGCCAGGGA GCACTGGGGG ACTGCTGGCT CTTGGCGGCC 
ATCGCCTCCC TCACTCTCAA CGACACCCTC CTGCACCGAG TGGTTCCGCA CGGCCAGAGC 
TTCCAGAATG GCTATGCCGG CATCTTCCAT TTCCAGCTGT GGCAATTTGG GGAGTGGGTG 
GACGTGGTCG TGGATGACCT GCTGCCCATC AAGGACGGGA AGCTAGTGTT CGTGCACTCT 
GCCGAAGGCA ACGAGTTCTG GAGCGCCCTG CTTGAGAAGG CCTATGCCAA GGTAAATGGC 
AGCTACGAGG CCCTGTCAGG GGGCAGCACC TCAGAGGGCT TTGAGGACTT CACAGGCGGG 
GTTACCGAGT GGTACGAGTT GCGCAAGGCT CCCAGTGACC TCTACCAGAT CATCCTCAAG 
GCGCTGGAGC GGGGCTCCCT GCTGGGCTGC TCCATAGACA TCTCCAGCGT TCTAGACATG 
GAGGCCATCA CTTTCAAGAA GTTGGTGAAG GGCCATGCCT ACTCTGTGAC CGGGGCCAAG 
CAGGTGAACT ACCGAGGCCA GGTGGTGAGC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CGGGAGCCTG GAGCGACAGC TCCTCAGAGT GGAACAACGT GGACCCATAT 
GAACGGGACC AGCTCCGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC ATTCCGAGAC 
TTCATGCGGG AGTTCACCCG CCTGGAGATC TGCAACCTCA CACCCGACGC CCTCAAGAGC 
CGGACCATCC GCAAATGGAA CACCACACTC TACGAAGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGAAACTA CCCAGCCACC TTCTGGGTGA ACCCTCAGTT CAAGATCCGG 
CTGGATGAGA CGGATGACCC GGACGACTAC GGGGACCGCG AGTCAGGCTG CAGCTTCGTG 
CTCGCCCTTA TGCAGAAGCA CCGTCGCCGC GAGCGCCGCT TCGGCCGCGA CATGGAGACT 
ATTGGCTTCG CGGTCTACGA GGTCCCTCCG GAGCTGGTGG GCCAGCCGGC CGTACACTTG 
AAGCGTGACT TCTTCCTGGC CAATGCGTCT CGGGCGCGCT CAGAGCAGTT CATCAACCTG 
CGAGAGGTCA GCACCCGCTT CCGCCTGCCA CCCGGGGAGT ATGTGGTGGT GCCCTCCACC 
TTCGAGCCCA ACAAGGAGGG CGACTTCGTG CTGCGCTTCT TCTCAGAGAA GAGTGCTGGG 
ACTGTGGAGC TGGATGACCA GATCCAGGCC AATCTCCCCG ATGAGCAAGT GCTCTCAGAA 
GAGGAGATTG ACGAGAACTT CAAGGCCCTC TTCAGGCAGC TGGCAGGGGA GGACATGGAG 
ATCAGCGTGA AGGAGTTGCG GACAATCCTC AATAGGATCA TCAGCAAACA CAAAGACCTG 
CGGACCAAGG GCTTCAGCCT AGAGTCGTGC CGCAGCATGG TGAACCTCAT GGATCGTGAT 
GGCAATGGGA AGCTGGGCCT GGTGGAGTTC AACATCCTGT GGAACCGCAT CCGGAATTAC 
CTGTCCATCT TCCGGAAGTT TGACCTGGAC AAGTCGGGCA GCATGAGTGC CTACGAGATG 
CGGATGGCCA TTGAGTCGGC AGGCTTCAAG CTCAACAAGA AGCTGTACGA GCTCATCATC 
ACCCGCTACT CGGAGCCCGA CCTGGCGGTC GACTTTGACA ATTTCGTTTG CTGCCTGGTG 
CGGCTAGAGA CCATGTTCCG ATTTTTCAAA ACTCTGGACA CAGATCTGGA TGGAGTTGTG 
ACCTTTGACT TGTTTAAGTG GTTGCAGCTG ACCATGTTTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN1 ( NM_005186.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005177.2 Homo sapiens calpain 1 (CAPN1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16773
Clone ID Related Accession (Same CDS sequence)	XM_011545292.1 , XM_006718698.2 , NM_001198869.1 , NM_001198868.1 , NM_005186.4 , NM_001198868.2 , NM_005186.3 , XM_006718698.1
Accession Version	NM_005186.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	calpain-1 catalytic subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE018156.1, BC008751.2 and X04366.1. On Nov 11, 2010 this sequence version replaced NM_005186.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC008751.2, X04366.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAGG AGATCATCAC GCCGGTGTAC TGCACTGGGG TGTCAGCCCA AGTGCAGAAG 
CAGCGGGCCA GGGAGCTGGG CCTGGGCCGC CATGAGAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAGC AGCTGCGGGT GCGATGCCTG CAGAGTGGGA CCCTCTTCCG TGATGAGGCC 
TTCCCCCCGG TACCCCAGAG CCTGGGTTAC AAGGACCTGG GTCCCAATTC CTCCAAGACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAA CTGCTGTCAA ACCCCCAGTT CATTGTGGAT 
GGAGCTACCC GCACAGACAT CTGCCAGGGA GCACTGGGGG ACTGCTGGCT CTTGGCGGCC 
ATCGCCTCCC TCACTCTCAA CGACACCCTC CTGCACCGAG TGGTTCCGCA CGGCCAGAGC 
TTCCAGAATG GCTATGCCGG CATCTTCCAT TTCCAGCTGT GGCAATTTGG GGAGTGGGTG 
GACGTGGTCG TGGATGACCT GCTGCCCATC AAGGACGGGA AGCTAGTGTT CGTGCACTCT 
GCCGAAGGCA ACGAGTTCTG GAGCGCCCTG CTTGAGAAGG CCTATGCCAA GGTAAATGGC 
AGCTACGAGG CCCTGTCAGG GGGCAGCACC TCAGAGGGCT TTGAGGACTT CACAGGCGGG 
GTTACCGAGT GGTACGAGTT GCGCAAGGCT CCCAGTGACC TCTACCAGAT CATCCTCAAG 
GCGCTGGAGC GGGGCTCCCT GCTGGGCTGC TCCATAGACA TCTCCAGCGT TCTAGACATG 
GAGGCCATCA CTTTCAAGAA GTTGGTGAAG GGCCATGCCT ACTCTGTGAC CGGGGCCAAG 
CAGGTGAACT ACCGAGGCCA GGTGGTGAGC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CGGGAGCCTG GAGCGACAGC TCCTCAGAGT GGAACAACGT GGACCCATAT 
GAACGGGACC AGCTCCGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC ATTCCGAGAC 
TTCATGCGGG AGTTCACCCG CCTGGAGATC TGCAACCTCA CACCCGACGC CCTCAAGAGC 
CGGACCATCC GCAAATGGAA CACCACACTC TACGAAGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGAAACTA CCCAGCCACC TTCTGGGTGA ACCCTCAGTT CAAGATCCGG 
CTGGATGAGA CGGATGACCC GGACGACTAC GGGGACCGCG AGTCAGGCTG CAGCTTCGTG 
CTCGCCCTTA TGCAGAAGCA CCGTCGCCGC GAGCGCCGCT TCGGCCGCGA CATGGAGACT 
ATTGGCTTCG CGGTCTACGA GGTCCCTCCG GAGCTGGTGG GCCAGCCGGC CGTACACTTG 
AAGCGTGACT TCTTCCTGGC CAATGCGTCT CGGGCGCGCT CAGAGCAGTT CATCAACCTG 
CGAGAGGTCA GCACCCGCTT CCGCCTGCCA CCCGGGGAGT ATGTGGTGGT GCCCTCCACC 
TTCGAGCCCA ACAAGGAGGG CGACTTCGTG CTGCGCTTCT TCTCAGAGAA GAGTGCTGGG 
ACTGTGGAGC TGGATGACCA GATCCAGGCC AATCTCCCCG ATGAGCAAGT GCTCTCAGAA 
GAGGAGATTG ACGAGAACTT CAAGGCCCTC TTCAGGCAGC TGGCAGGGGA GGACATGGAG 
ATCAGCGTGA AGGAGTTGCG GACAATCCTC AATAGGATCA TCAGCAAACA CAAAGACCTG 
CGGACCAAGG GCTTCAGCCT AGAGTCGTGC CGCAGCATGG TGAACCTCAT GGATCGTGAT 
GGCAATGGGA AGCTGGGCCT GGTGGAGTTC AACATCCTGT GGAACCGCAT CCGGAATTAC 
CTGTCCATCT TCCGGAAGTT TGACCTGGAC AAGTCGGGCA GCATGAGTGC CTACGAGATG 
CGGATGGCCA TTGAGTCGGC AGGCTTCAAG CTCAACAAGA AGCTGTACGA GCTCATCATC 
ACCCGCTACT CGGAGCCCGA CCTGGCGGTC GACTTTGACA ATTTCGTTTG CTGCCTGGTG 
CGGCTAGAGA CCATGTTCCG ATTTTTCAAA ACTCTGGACA CAGATCTGGA TGGAGTTGTG 
ACCTTTGACT TGTTTAAGTG GTTGCAGCTG ACCATGTTTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005177.2
CDS	183..2327
Translation	MSEEIITPVYCTGVSAQVQKQRARELGLGRHENAIKYLGQDYEQLRVRCLQSGTLFRDEAFPPVPQSLGYKDLGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDLYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQVNYRGQVVSLIRMRNPWGEVEWTGAWSDSSSEWNNVDPYERDQLRVKMEDGEFWMSFRDFMREFTRLEICNLTPDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPDEQVLSEEEIDENFKALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA

Target ORF information:

RefSeq Version	NM_005186.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 1 (CAPN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005186.3

ORF Insert Sequence:

ATGTCGGAGG AGATCATCAC GCCGGTGTAC TGCACTGGGG TGTCAGCCCA AGTGCAGAAG 
CAGCGGGCCA GGGAGCTGGG CCTGGGCCGC CATGAGAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAGC AGCTGCGGGT GCGATGCCTG CAGAGTGGGA CCCTCTTCCG TGATGAGGCC 
TTCCCCCCGG TACCCCAGAG CCTGGGTTAC AAGGACCTGG GTCCCAATTC CTCCAAGACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAA CTGCTGTCAA ACCCCCAGTT CATTGTGGAT 
GGAGCTACCC GCACAGACAT CTGCCAGGGA GCACTGGGGG ACTGCTGGCT CTTGGCGGCC 
ATCGCCTCCC TCACTCTCAA CGACACCCTC CTGCACCGAG TGGTTCCGCA CGGCCAGAGC 
TTCCAGAATG GCTATGCCGG CATCTTCCAT TTCCAGCTGT GGCAATTTGG GGAGTGGGTG 
GACGTGGTCG TGGATGACCT GCTGCCCATC AAGGACGGGA AGCTAGTGTT CGTGCACTCT 
GCCGAAGGCA ACGAGTTCTG GAGCGCCCTG CTTGAGAAGG CCTATGCCAA GGTAAATGGC 
AGCTACGAGG CCCTGTCAGG GGGCAGCACC TCAGAGGGCT TTGAGGACTT CACAGGCGGG 
GTTACCGAGT GGTACGAGTT GCGCAAGGCT CCCAGTGACC TCTACCAGAT CATCCTCAAG 
GCGCTGGAGC GGGGCTCCCT GCTGGGCTGC TCCATAGACA TCTCCAGCGT TCTAGACATG 
GAGGCCATCA CTTTCAAGAA GTTGGTGAAG GGCCATGCCT ACTCTGTGAC CGGGGCCAAG 
CAGGTGAACT ACCGAGGCCA GGTGGTGAGC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CGGGAGCCTG GAGCGACAGC TCCTCAGAGT GGAACAACGT GGACCCATAT 
GAACGGGACC AGCTCCGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC ATTCCGAGAC 
TTCATGCGGG AGTTCACCCG CCTGGAGATC TGCAACCTCA CACCCGACGC CCTCAAGAGC 
CGGACCATCC GCAAATGGAA CACCACACTC TACGAAGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGAAACTA CCCAGCCACC TTCTGGGTGA ACCCTCAGTT CAAGATCCGG 
CTGGATGAGA CGGATGACCC GGACGACTAC GGGGACCGCG AGTCAGGCTG CAGCTTCGTG 
CTCGCCCTTA TGCAGAAGCA CCGTCGCCGC GAGCGCCGCT TCGGCCGCGA CATGGAGACT 
ATTGGCTTCG CGGTCTACGA GGTCCCTCCG GAGCTGGTGG GCCAGCCGGC CGTACACTTG 
AAGCGTGACT TCTTCCTGGC CAATGCGTCT CGGGCGCGCT CAGAGCAGTT CATCAACCTG 
CGAGAGGTCA GCACCCGCTT CCGCCTGCCA CCCGGGGAGT ATGTGGTGGT GCCCTCCACC 
TTCGAGCCCA ACAAGGAGGG CGACTTCGTG CTGCGCTTCT TCTCAGAGAA GAGTGCTGGG 
ACTGTGGAGC TGGATGACCA GATCCAGGCC AATCTCCCCG ATGAGCAAGT GCTCTCAGAA 
GAGGAGATTG ACGAGAACTT CAAGGCCCTC TTCAGGCAGC TGGCAGGGGA GGACATGGAG 
ATCAGCGTGA AGGAGTTGCG GACAATCCTC AATAGGATCA TCAGCAAACA CAAAGACCTG 
CGGACCAAGG GCTTCAGCCT AGAGTCGTGC CGCAGCATGG TGAACCTCAT GGATCGTGAT 
GGCAATGGGA AGCTGGGCCT GGTGGAGTTC AACATCCTGT GGAACCGCAT CCGGAATTAC 
CTGTCCATCT TCCGGAAGTT TGACCTGGAC AAGTCGGGCA GCATGAGTGC CTACGAGATG 
CGGATGGCCA TTGAGTCGGC AGGCTTCAAG CTCAACAAGA AGCTGTACGA GCTCATCATC 
ACCCGCTACT CGGAGCCCGA CCTGGCGGTC GACTTTGACA ATTTCGTTTG CTGCCTGGTG 
CGGCTAGAGA CCATGTTCCG ATTTTTCAAA ACTCTGGACA CAGATCTGGA TGGAGTTGTG 
ACCTTTGACT TGTTTAAGTG GTTGCAGCTG ACCATGTTTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN1 ( XM_011545292.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543594.1 Homo sapiens calpain 1 (CAPN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu16773
Clone ID Related Accession (Same CDS sequence)	XM_011545292.1 , XM_006718698.2 , NM_001198869.1 , NM_001198868.1 , NM_005186.4 , NM_001198868.2 , NM_005186.3 , XM_006718698.1
Accession Version	XM_011545292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	calpain-1 catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGG AGATCATCAC GCCGGTGTAC TGCACTGGGG TGTCAGCCCA AGTGCAGAAG 
CAGCGGGCCA GGGAGCTGGG CCTGGGCCGC CATGAGAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAGC AGCTGCGGGT GCGATGCCTG CAGAGTGGGA CCCTCTTCCG TGATGAGGCC 
TTCCCCCCGG TACCCCAGAG CCTGGGTTAC AAGGACCTGG GTCCCAATTC CTCCAAGACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAA CTGCTGTCAA ACCCCCAGTT CATTGTGGAT 
GGAGCTACCC GCACAGACAT CTGCCAGGGA GCACTGGGGG ACTGCTGGCT CTTGGCGGCC 
ATCGCCTCCC TCACTCTCAA CGACACCCTC CTGCACCGAG TGGTTCCGCA CGGCCAGAGC 
TTCCAGAATG GCTATGCCGG CATCTTCCAT TTCCAGCTGT GGCAATTTGG GGAGTGGGTG 
GACGTGGTCG TGGATGACCT GCTGCCCATC AAGGACGGGA AGCTAGTGTT CGTGCACTCT 
GCCGAAGGCA ACGAGTTCTG GAGCGCCCTG CTTGAGAAGG CCTATGCCAA GGTAAATGGC 
AGCTACGAGG CCCTGTCAGG GGGCAGCACC TCAGAGGGCT TTGAGGACTT CACAGGCGGG 
GTTACCGAGT GGTACGAGTT GCGCAAGGCT CCCAGTGACC TCTACCAGAT CATCCTCAAG 
GCGCTGGAGC GGGGCTCCCT GCTGGGCTGC TCCATAGACA TCTCCAGCGT TCTAGACATG 
GAGGCCATCA CTTTCAAGAA GTTGGTGAAG GGCCATGCCT ACTCTGTGAC CGGGGCCAAG 
CAGGTGAACT ACCGAGGCCA GGTGGTGAGC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CGGGAGCCTG GAGCGACAGC TCCTCAGAGT GGAACAACGT GGACCCATAT 
GAACGGGACC AGCTCCGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC ATTCCGAGAC 
TTCATGCGGG AGTTCACCCG CCTGGAGATC TGCAACCTCA CACCCGACGC CCTCAAGAGC 
CGGACCATCC GCAAATGGAA CACCACACTC TACGAAGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGAAACTA CCCAGCCACC TTCTGGGTGA ACCCTCAGTT CAAGATCCGG 
CTGGATGAGA CGGATGACCC GGACGACTAC GGGGACCGCG AGTCAGGCTG CAGCTTCGTG 
CTCGCCCTTA TGCAGAAGCA CCGTCGCCGC GAGCGCCGCT TCGGCCGCGA CATGGAGACT 
ATTGGCTTCG CGGTCTACGA GGTCCCTCCG GAGCTGGTGG GCCAGCCGGC CGTACACTTG 
AAGCGTGACT TCTTCCTGGC CAATGCGTCT CGGGCGCGCT CAGAGCAGTT CATCAACCTG 
CGAGAGGTCA GCACCCGCTT CCGCCTGCCA CCCGGGGAGT ATGTGGTGGT GCCCTCCACC 
TTCGAGCCCA ACAAGGAGGG CGACTTCGTG CTGCGCTTCT TCTCAGAGAA GAGTGCTGGG 
ACTGTGGAGC TGGATGACCA GATCCAGGCC AATCTCCCCG ATGAGCAAGT GCTCTCAGAA 
GAGGAGATTG ACGAGAACTT CAAGGCCCTC TTCAGGCAGC TGGCAGGGGA GGACATGGAG 
ATCAGCGTGA AGGAGTTGCG GACAATCCTC AATAGGATCA TCAGCAAACA CAAAGACCTG 
CGGACCAAGG GCTTCAGCCT AGAGTCGTGC CGCAGCATGG TGAACCTCAT GGATCGTGAT 
GGCAATGGGA AGCTGGGCCT GGTGGAGTTC AACATCCTGT GGAACCGCAT CCGGAATTAC 
CTGTCCATCT TCCGGAAGTT TGACCTGGAC AAGTCGGGCA GCATGAGTGC CTACGAGATG 
CGGATGGCCA TTGAGTCGGC AGGCTTCAAG CTCAACAAGA AGCTGTACGA GCTCATCATC 
ACCCGCTACT CGGAGCCCGA CCTGGCGGTC GACTTTGACA ATTTCGTTTG CTGCCTGGTG 
CGGCTAGAGA CCATGTTCCG ATTTTTCAAA ACTCTGGACA CAGATCTGGA TGGAGTTGTG 
ACCTTTGACT TGTTTAAGTG GTTGCAGCTG ACCATGTTTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543594.1
CDS	109..2253
Translation	MSEEIITPVYCTGVSAQVQKQRARELGLGRHENAIKYLGQDYEQLRVRCLQSGTLFRDEAFPPVPQSLGYKDLGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDLYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQVNYRGQVVSLIRMRNPWGEVEWTGAWSDSSSEWNNVDPYERDQLRVKMEDGEFWMSFRDFMREFTRLEICNLTPDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPDEQVLSEEEIDENFKALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA

Target ORF information:

RefSeq Version	XM_011545292.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 1 (CAPN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545292.1

ORF Insert Sequence:

ATGTCGGAGG AGATCATCAC GCCGGTGTAC TGCACTGGGG TGTCAGCCCA AGTGCAGAAG 
CAGCGGGCCA GGGAGCTGGG CCTGGGCCGC CATGAGAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAGC AGCTGCGGGT GCGATGCCTG CAGAGTGGGA CCCTCTTCCG TGATGAGGCC 
TTCCCCCCGG TACCCCAGAG CCTGGGTTAC AAGGACCTGG GTCCCAATTC CTCCAAGACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAA CTGCTGTCAA ACCCCCAGTT CATTGTGGAT 
GGAGCTACCC GCACAGACAT CTGCCAGGGA GCACTGGGGG ACTGCTGGCT CTTGGCGGCC 
ATCGCCTCCC TCACTCTCAA CGACACCCTC CTGCACCGAG TGGTTCCGCA CGGCCAGAGC 
TTCCAGAATG GCTATGCCGG CATCTTCCAT TTCCAGCTGT GGCAATTTGG GGAGTGGGTG 
GACGTGGTCG TGGATGACCT GCTGCCCATC AAGGACGGGA AGCTAGTGTT CGTGCACTCT 
GCCGAAGGCA ACGAGTTCTG GAGCGCCCTG CTTGAGAAGG CCTATGCCAA GGTAAATGGC 
AGCTACGAGG CCCTGTCAGG GGGCAGCACC TCAGAGGGCT TTGAGGACTT CACAGGCGGG 
GTTACCGAGT GGTACGAGTT GCGCAAGGCT CCCAGTGACC TCTACCAGAT CATCCTCAAG 
GCGCTGGAGC GGGGCTCCCT GCTGGGCTGC TCCATAGACA TCTCCAGCGT TCTAGACATG 
GAGGCCATCA CTTTCAAGAA GTTGGTGAAG GGCCATGCCT ACTCTGTGAC CGGGGCCAAG 
CAGGTGAACT ACCGAGGCCA GGTGGTGAGC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CGGGAGCCTG GAGCGACAGC TCCTCAGAGT GGAACAACGT GGACCCATAT 
GAACGGGACC AGCTCCGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC ATTCCGAGAC 
TTCATGCGGG AGTTCACCCG CCTGGAGATC TGCAACCTCA CACCCGACGC CCTCAAGAGC 
CGGACCATCC GCAAATGGAA CACCACACTC TACGAAGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGAAACTA CCCAGCCACC TTCTGGGTGA ACCCTCAGTT CAAGATCCGG 
CTGGATGAGA CGGATGACCC GGACGACTAC GGGGACCGCG AGTCAGGCTG CAGCTTCGTG 
CTCGCCCTTA TGCAGAAGCA CCGTCGCCGC GAGCGCCGCT TCGGCCGCGA CATGGAGACT 
ATTGGCTTCG CGGTCTACGA GGTCCCTCCG GAGCTGGTGG GCCAGCCGGC CGTACACTTG 
AAGCGTGACT TCTTCCTGGC CAATGCGTCT CGGGCGCGCT CAGAGCAGTT CATCAACCTG 
CGAGAGGTCA GCACCCGCTT CCGCCTGCCA CCCGGGGAGT ATGTGGTGGT GCCCTCCACC 
TTCGAGCCCA ACAAGGAGGG CGACTTCGTG CTGCGCTTCT TCTCAGAGAA GAGTGCTGGG 
ACTGTGGAGC TGGATGACCA GATCCAGGCC AATCTCCCCG ATGAGCAAGT GCTCTCAGAA 
GAGGAGATTG ACGAGAACTT CAAGGCCCTC TTCAGGCAGC TGGCAGGGGA GGACATGGAG 
ATCAGCGTGA AGGAGTTGCG GACAATCCTC AATAGGATCA TCAGCAAACA CAAAGACCTG 
CGGACCAAGG GCTTCAGCCT AGAGTCGTGC CGCAGCATGG TGAACCTCAT GGATCGTGAT 
GGCAATGGGA AGCTGGGCCT GGTGGAGTTC AACATCCTGT GGAACCGCAT CCGGAATTAC 
CTGTCCATCT TCCGGAAGTT TGACCTGGAC AAGTCGGGCA GCATGAGTGC CTACGAGATG 
CGGATGGCCA TTGAGTCGGC AGGCTTCAAG CTCAACAAGA AGCTGTACGA GCTCATCATC 
ACCCGCTACT CGGAGCCCGA CCTGGCGGTC GACTTTGACA ATTTCGTTTG CTGCCTGGTG 
CGGCTAGAGA CCATGTTCCG ATTTTTCAAA ACTCTGGACA CAGATCTGGA TGGAGTTGTG 
ACCTTTGACT TGTTTAAGTG GTTGCAGCTG ACCATGTTTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN1 ( XM_006718698.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718761.1 Homo sapiens calpain 1 (CAPN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu16773
Clone ID Related Accession (Same CDS sequence)	XM_011545292.1 , XM_006718698.2 , NM_001198869.1 , NM_001198868.1 , NM_005186.4 , NM_001198868.2 , NM_005186.3 , XM_006718698.1
Accession Version	XM_006718698.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	calpain-1 catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006718698.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGG AGATCATCAC GCCGGTGTAC TGCACTGGGG TGTCAGCCCA AGTGCAGAAG 
CAGCGGGCCA GGGAGCTGGG CCTGGGCCGC CATGAGAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAGC AGCTGCGGGT GCGATGCCTG CAGAGTGGGA CCCTCTTCCG TGATGAGGCC 
TTCCCCCCGG TACCCCAGAG CCTGGGTTAC AAGGACCTGG GTCCCAATTC CTCCAAGACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAA CTGCTGTCAA ACCCCCAGTT CATTGTGGAT 
GGAGCTACCC GCACAGACAT CTGCCAGGGA GCACTGGGGG ACTGCTGGCT CTTGGCGGCC 
ATCGCCTCCC TCACTCTCAA CGACACCCTC CTGCACCGAG TGGTTCCGCA CGGCCAGAGC 
TTCCAGAATG GCTATGCCGG CATCTTCCAT TTCCAGCTGT GGCAATTTGG GGAGTGGGTG 
GACGTGGTCG TGGATGACCT GCTGCCCATC AAGGACGGGA AGCTAGTGTT CGTGCACTCT 
GCCGAAGGCA ACGAGTTCTG GAGCGCCCTG CTTGAGAAGG CCTATGCCAA GGTAAATGGC 
AGCTACGAGG CCCTGTCAGG GGGCAGCACC TCAGAGGGCT TTGAGGACTT CACAGGCGGG 
GTTACCGAGT GGTACGAGTT GCGCAAGGCT CCCAGTGACC TCTACCAGAT CATCCTCAAG 
GCGCTGGAGC GGGGCTCCCT GCTGGGCTGC TCCATAGACA TCTCCAGCGT TCTAGACATG 
GAGGCCATCA CTTTCAAGAA GTTGGTGAAG GGCCATGCCT ACTCTGTGAC CGGGGCCAAG 
CAGGTGAACT ACCGAGGCCA GGTGGTGAGC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CGGGAGCCTG GAGCGACAGC TCCTCAGAGT GGAACAACGT GGACCCATAT 
GAACGGGACC AGCTCCGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC ATTCCGAGAC 
TTCATGCGGG AGTTCACCCG CCTGGAGATC TGCAACCTCA CACCCGACGC CCTCAAGAGC 
CGGACCATCC GCAAATGGAA CACCACACTC TACGAAGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGAAACTA CCCAGCCACC TTCTGGGTGA ACCCTCAGTT CAAGATCCGG 
CTGGATGAGA CGGATGACCC GGACGACTAC GGGGACCGCG AGTCAGGCTG CAGCTTCGTG 
CTCGCCCTTA TGCAGAAGCA CCGTCGCCGC GAGCGCCGCT TCGGCCGCGA CATGGAGACT 
ATTGGCTTCG CGGTCTACGA GGTCCCTCCG GAGCTGGTGG GCCAGCCGGC CGTACACTTG 
AAGCGTGACT TCTTCCTGGC CAATGCGTCT CGGGCGCGCT CAGAGCAGTT CATCAACCTG 
CGAGAGGTCA GCACCCGCTT CCGCCTGCCA CCCGGGGAGT ATGTGGTGGT GCCCTCCACC 
TTCGAGCCCA ACAAGGAGGG CGACTTCGTG CTGCGCTTCT TCTCAGAGAA GAGTGCTGGG 
ACTGTGGAGC TGGATGACCA GATCCAGGCC AATCTCCCCG ATGAGCAAGT GCTCTCAGAA 
GAGGAGATTG ACGAGAACTT CAAGGCCCTC TTCAGGCAGC TGGCAGGGGA GGACATGGAG 
ATCAGCGTGA AGGAGTTGCG GACAATCCTC AATAGGATCA TCAGCAAACA CAAAGACCTG 
CGGACCAAGG GCTTCAGCCT AGAGTCGTGC CGCAGCATGG TGAACCTCAT GGATCGTGAT 
GGCAATGGGA AGCTGGGCCT GGTGGAGTTC AACATCCTGT GGAACCGCAT CCGGAATTAC 
CTGTCCATCT TCCGGAAGTT TGACCTGGAC AAGTCGGGCA GCATGAGTGC CTACGAGATG 
CGGATGGCCA TTGAGTCGGC AGGCTTCAAG CTCAACAAGA AGCTGTACGA GCTCATCATC 
ACCCGCTACT CGGAGCCCGA CCTGGCGGTC GACTTTGACA ATTTCGTTTG CTGCCTGGTG 
CGGCTAGAGA CCATGTTCCG ATTTTTCAAA ACTCTGGACA CAGATCTGGA TGGAGTTGTG 
ACCTTTGACT TGTTTAAGTG GTTGCAGCTG ACCATGTTTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718761.1
CDS	78..2222
Translation	MSEEIITPVYCTGVSAQVQKQRARELGLGRHENAIKYLGQDYEQLRVRCLQSGTLFRDEAFPPVPQSLGYKDLGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDLYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQVNYRGQVVSLIRMRNPWGEVEWTGAWSDSSSEWNNVDPYERDQLRVKMEDGEFWMSFRDFMREFTRLEICNLTPDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPDEQVLSEEEIDENFKALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA

Target ORF information:

RefSeq Version	XM_006718698.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 1 (CAPN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718698.2

ORF Insert Sequence:

ATGTCGGAGG AGATCATCAC GCCGGTGTAC TGCACTGGGG TGTCAGCCCA AGTGCAGAAG 
CAGCGGGCCA GGGAGCTGGG CCTGGGCCGC CATGAGAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAGC AGCTGCGGGT GCGATGCCTG CAGAGTGGGA CCCTCTTCCG TGATGAGGCC 
TTCCCCCCGG TACCCCAGAG CCTGGGTTAC AAGGACCTGG GTCCCAATTC CTCCAAGACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAA CTGCTGTCAA ACCCCCAGTT CATTGTGGAT 
GGAGCTACCC GCACAGACAT CTGCCAGGGA GCACTGGGGG ACTGCTGGCT CTTGGCGGCC 
ATCGCCTCCC TCACTCTCAA CGACACCCTC CTGCACCGAG TGGTTCCGCA CGGCCAGAGC 
TTCCAGAATG GCTATGCCGG CATCTTCCAT TTCCAGCTGT GGCAATTTGG GGAGTGGGTG 
GACGTGGTCG TGGATGACCT GCTGCCCATC AAGGACGGGA AGCTAGTGTT CGTGCACTCT 
GCCGAAGGCA ACGAGTTCTG GAGCGCCCTG CTTGAGAAGG CCTATGCCAA GGTAAATGGC 
AGCTACGAGG CCCTGTCAGG GGGCAGCACC TCAGAGGGCT TTGAGGACTT CACAGGCGGG 
GTTACCGAGT GGTACGAGTT GCGCAAGGCT CCCAGTGACC TCTACCAGAT CATCCTCAAG 
GCGCTGGAGC GGGGCTCCCT GCTGGGCTGC TCCATAGACA TCTCCAGCGT TCTAGACATG 
GAGGCCATCA CTTTCAAGAA GTTGGTGAAG GGCCATGCCT ACTCTGTGAC CGGGGCCAAG 
CAGGTGAACT ACCGAGGCCA GGTGGTGAGC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CGGGAGCCTG GAGCGACAGC TCCTCAGAGT GGAACAACGT GGACCCATAT 
GAACGGGACC AGCTCCGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC ATTCCGAGAC 
TTCATGCGGG AGTTCACCCG CCTGGAGATC TGCAACCTCA CACCCGACGC CCTCAAGAGC 
CGGACCATCC GCAAATGGAA CACCACACTC TACGAAGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGAAACTA CCCAGCCACC TTCTGGGTGA ACCCTCAGTT CAAGATCCGG 
CTGGATGAGA CGGATGACCC GGACGACTAC GGGGACCGCG AGTCAGGCTG CAGCTTCGTG 
CTCGCCCTTA TGCAGAAGCA CCGTCGCCGC GAGCGCCGCT TCGGCCGCGA CATGGAGACT 
ATTGGCTTCG CGGTCTACGA GGTCCCTCCG GAGCTGGTGG GCCAGCCGGC CGTACACTTG 
AAGCGTGACT TCTTCCTGGC CAATGCGTCT CGGGCGCGCT CAGAGCAGTT CATCAACCTG 
CGAGAGGTCA GCACCCGCTT CCGCCTGCCA CCCGGGGAGT ATGTGGTGGT GCCCTCCACC 
TTCGAGCCCA ACAAGGAGGG CGACTTCGTG CTGCGCTTCT TCTCAGAGAA GAGTGCTGGG 
ACTGTGGAGC TGGATGACCA GATCCAGGCC AATCTCCCCG ATGAGCAAGT GCTCTCAGAA 
GAGGAGATTG ACGAGAACTT CAAGGCCCTC TTCAGGCAGC TGGCAGGGGA GGACATGGAG 
ATCAGCGTGA AGGAGTTGCG GACAATCCTC AATAGGATCA TCAGCAAACA CAAAGACCTG 
CGGACCAAGG GCTTCAGCCT AGAGTCGTGC CGCAGCATGG TGAACCTCAT GGATCGTGAT 
GGCAATGGGA AGCTGGGCCT GGTGGAGTTC AACATCCTGT GGAACCGCAT CCGGAATTAC 
CTGTCCATCT TCCGGAAGTT TGACCTGGAC AAGTCGGGCA GCATGAGTGC CTACGAGATG 
CGGATGGCCA TTGAGTCGGC AGGCTTCAAG CTCAACAAGA AGCTGTACGA GCTCATCATC 
ACCCGCTACT CGGAGCCCGA CCTGGCGGTC GACTTTGACA ATTTCGTTTG CTGCCTGGTG 
CGGCTAGAGA CCATGTTCCG ATTTTTCAAA ACTCTGGACA CAGATCTGGA TGGAGTTGTG 
ACCTTTGACT TGTTTAAGTG GTTGCAGCTG ACCATGTTTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN1 ( XM_006718698.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718761.1 Homo sapiens calpain 1 (CAPN1), transcript variant X1, mRNA.

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CloneID	OHu16773
Clone ID Related Accession (Same CDS sequence)	XM_011545292.1 , XM_006718698.2 , NM_001198869.1 , NM_001198868.1 , NM_005186.4 , NM_001198868.2 , NM_005186.3 , XM_006718698.1
Accession Version	XM_006718698.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpain-1 catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGG AGATCATCAC GCCGGTGTAC TGCACTGGGG TGTCAGCCCA AGTGCAGAAG 
CAGCGGGCCA GGGAGCTGGG CCTGGGCCGC CATGAGAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAGC AGCTGCGGGT GCGATGCCTG CAGAGTGGGA CCCTCTTCCG TGATGAGGCC 
TTCCCCCCGG TACCCCAGAG CCTGGGTTAC AAGGACCTGG GTCCCAATTC CTCCAAGACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAA CTGCTGTCAA ACCCCCAGTT CATTGTGGAT 
GGAGCTACCC GCACAGACAT CTGCCAGGGA GCACTGGGGG ACTGCTGGCT CTTGGCGGCC 
ATCGCCTCCC TCACTCTCAA CGACACCCTC CTGCACCGAG TGGTTCCGCA CGGCCAGAGC 
TTCCAGAATG GCTATGCCGG CATCTTCCAT TTCCAGCTGT GGCAATTTGG GGAGTGGGTG 
GACGTGGTCG TGGATGACCT GCTGCCCATC AAGGACGGGA AGCTAGTGTT CGTGCACTCT 
GCCGAAGGCA ACGAGTTCTG GAGCGCCCTG CTTGAGAAGG CCTATGCCAA GGTAAATGGC 
AGCTACGAGG CCCTGTCAGG GGGCAGCACC TCAGAGGGCT TTGAGGACTT CACAGGCGGG 
GTTACCGAGT GGTACGAGTT GCGCAAGGCT CCCAGTGACC TCTACCAGAT CATCCTCAAG 
GCGCTGGAGC GGGGCTCCCT GCTGGGCTGC TCCATAGACA TCTCCAGCGT TCTAGACATG 
GAGGCCATCA CTTTCAAGAA GTTGGTGAAG GGCCATGCCT ACTCTGTGAC CGGGGCCAAG 
CAGGTGAACT ACCGAGGCCA GGTGGTGAGC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CGGGAGCCTG GAGCGACAGC TCCTCAGAGT GGAACAACGT GGACCCATAT 
GAACGGGACC AGCTCCGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC ATTCCGAGAC 
TTCATGCGGG AGTTCACCCG CCTGGAGATC TGCAACCTCA CACCCGACGC CCTCAAGAGC 
CGGACCATCC GCAAATGGAA CACCACACTC TACGAAGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGAAACTA CCCAGCCACC TTCTGGGTGA ACCCTCAGTT CAAGATCCGG 
CTGGATGAGA CGGATGACCC GGACGACTAC GGGGACCGCG AGTCAGGCTG CAGCTTCGTG 
CTCGCCCTTA TGCAGAAGCA CCGTCGCCGC GAGCGCCGCT TCGGCCGCGA CATGGAGACT 
ATTGGCTTCG CGGTCTACGA GGTCCCTCCG GAGCTGGTGG GCCAGCCGGC CGTACACTTG 
AAGCGTGACT TCTTCCTGGC CAATGCGTCT CGGGCGCGCT CAGAGCAGTT CATCAACCTG 
CGAGAGGTCA GCACCCGCTT CCGCCTGCCA CCCGGGGAGT ATGTGGTGGT GCCCTCCACC 
TTCGAGCCCA ACAAGGAGGG CGACTTCGTG CTGCGCTTCT TCTCAGAGAA GAGTGCTGGG 
ACTGTGGAGC TGGATGACCA GATCCAGGCC AATCTCCCCG ATGAGCAAGT GCTCTCAGAA 
GAGGAGATTG ACGAGAACTT CAAGGCCCTC TTCAGGCAGC TGGCAGGGGA GGACATGGAG 
ATCAGCGTGA AGGAGTTGCG GACAATCCTC AATAGGATCA TCAGCAAACA CAAAGACCTG 
CGGACCAAGG GCTTCAGCCT AGAGTCGTGC CGCAGCATGG TGAACCTCAT GGATCGTGAT 
GGCAATGGGA AGCTGGGCCT GGTGGAGTTC AACATCCTGT GGAACCGCAT CCGGAATTAC 
CTGTCCATCT TCCGGAAGTT TGACCTGGAC AAGTCGGGCA GCATGAGTGC CTACGAGATG 
CGGATGGCCA TTGAGTCGGC AGGCTTCAAG CTCAACAAGA AGCTGTACGA GCTCATCATC 
ACCCGCTACT CGGAGCCCGA CCTGGCGGTC GACTTTGACA ATTTCGTTTG CTGCCTGGTG 
CGGCTAGAGA CCATGTTCCG ATTTTTCAAA ACTCTGGACA CAGATCTGGA TGGAGTTGTG 
ACCTTTGACT TGTTTAAGTG GTTGCAGCTG ACCATGTTTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718761.1
CDS	98..2242
Translation	MSEEIITPVYCTGVSAQVQKQRARELGLGRHENAIKYLGQDYEQLRVRCLQSGTLFRDEAFPPVPQSLGYKDLGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDLYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQVNYRGQVVSLIRMRNPWGEVEWTGAWSDSSSEWNNVDPYERDQLRVKMEDGEFWMSFRDFMREFTRLEICNLTPDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPDEQVLSEEEIDENFKALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA

Target ORF information:

RefSeq Version	XM_006718698.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 1 (CAPN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718698.1

ORF Insert Sequence:

ATGTCGGAGG AGATCATCAC GCCGGTGTAC TGCACTGGGG TGTCAGCCCA AGTGCAGAAG 
CAGCGGGCCA GGGAGCTGGG CCTGGGCCGC CATGAGAATG CCATCAAGTA CCTGGGCCAG 
GATTATGAGC AGCTGCGGGT GCGATGCCTG CAGAGTGGGA CCCTCTTCCG TGATGAGGCC 
TTCCCCCCGG TACCCCAGAG CCTGGGTTAC AAGGACCTGG GTCCCAATTC CTCCAAGACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAA CTGCTGTCAA ACCCCCAGTT CATTGTGGAT 
GGAGCTACCC GCACAGACAT CTGCCAGGGA GCACTGGGGG ACTGCTGGCT CTTGGCGGCC 
ATCGCCTCCC TCACTCTCAA CGACACCCTC CTGCACCGAG TGGTTCCGCA CGGCCAGAGC 
TTCCAGAATG GCTATGCCGG CATCTTCCAT TTCCAGCTGT GGCAATTTGG GGAGTGGGTG 
GACGTGGTCG TGGATGACCT GCTGCCCATC AAGGACGGGA AGCTAGTGTT CGTGCACTCT 
GCCGAAGGCA ACGAGTTCTG GAGCGCCCTG CTTGAGAAGG CCTATGCCAA GGTAAATGGC 
AGCTACGAGG CCCTGTCAGG GGGCAGCACC TCAGAGGGCT TTGAGGACTT CACAGGCGGG 
GTTACCGAGT GGTACGAGTT GCGCAAGGCT CCCAGTGACC TCTACCAGAT CATCCTCAAG 
GCGCTGGAGC GGGGCTCCCT GCTGGGCTGC TCCATAGACA TCTCCAGCGT TCTAGACATG 
GAGGCCATCA CTTTCAAGAA GTTGGTGAAG GGCCATGCCT ACTCTGTGAC CGGGGCCAAG 
CAGGTGAACT ACCGAGGCCA GGTGGTGAGC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CGGGAGCCTG GAGCGACAGC TCCTCAGAGT GGAACAACGT GGACCCATAT 
GAACGGGACC AGCTCCGGGT CAAGATGGAG GACGGGGAGT TCTGGATGTC ATTCCGAGAC 
TTCATGCGGG AGTTCACCCG CCTGGAGATC TGCAACCTCA CACCCGACGC CCTCAAGAGC 
CGGACCATCC GCAAATGGAA CACCACACTC TACGAAGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGAAACTA CCCAGCCACC TTCTGGGTGA ACCCTCAGTT CAAGATCCGG 
CTGGATGAGA CGGATGACCC GGACGACTAC GGGGACCGCG AGTCAGGCTG CAGCTTCGTG 
CTCGCCCTTA TGCAGAAGCA CCGTCGCCGC GAGCGCCGCT TCGGCCGCGA CATGGAGACT 
ATTGGCTTCG CGGTCTACGA GGTCCCTCCG GAGCTGGTGG GCCAGCCGGC CGTACACTTG 
AAGCGTGACT TCTTCCTGGC CAATGCGTCT CGGGCGCGCT CAGAGCAGTT CATCAACCTG 
CGAGAGGTCA GCACCCGCTT CCGCCTGCCA CCCGGGGAGT ATGTGGTGGT GCCCTCCACC 
TTCGAGCCCA ACAAGGAGGG CGACTTCGTG CTGCGCTTCT TCTCAGAGAA GAGTGCTGGG 
ACTGTGGAGC TGGATGACCA GATCCAGGCC AATCTCCCCG ATGAGCAAGT GCTCTCAGAA 
GAGGAGATTG ACGAGAACTT CAAGGCCCTC TTCAGGCAGC TGGCAGGGGA GGACATGGAG 
ATCAGCGTGA AGGAGTTGCG GACAATCCTC AATAGGATCA TCAGCAAACA CAAAGACCTG 
CGGACCAAGG GCTTCAGCCT AGAGTCGTGC CGCAGCATGG TGAACCTCAT GGATCGTGAT 
GGCAATGGGA AGCTGGGCCT GGTGGAGTTC AACATCCTGT GGAACCGCAT CCGGAATTAC 
CTGTCCATCT TCCGGAAGTT TGACCTGGAC AAGTCGGGCA GCATGAGTGC CTACGAGATG 
CGGATGGCCA TTGAGTCGGC AGGCTTCAAG CTCAACAAGA AGCTGTACGA GCTCATCATC 
ACCCGCTACT CGGAGCCCGA CCTGGCGGTC GACTTTGACA ATTTCGTTTG CTGCCTGGTG 
CGGCTAGAGA CCATGTTCCG ATTTTTCAAA ACTCTGGACA CAGATCTGGA TGGAGTTGTG 
ACCTTTGACT TGTTTAAGTG GTTGCAGCTG ACCATGTTTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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CAPN1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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