RTCB cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin)

Gene
Clones
gRNAs

Summary

Gene Symbol	RTCB
Entrez Gene ID	108501508
Full Name	RNA 2',3'-cyclic phosphate and 5'-OH ligase
Gene Type	protein-coding
Organism	Lepidothrix coronata(blue-crowned manakin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017823464.1	XP_017678953.1	tRNA-splicing ligase RtcB homolog

Pathways from BioSystems
Gene Ontology

The following RTCB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RTCB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLe64226	XM_017823464.1 Latest version!	Lepidothrix coronata RNA 2',3'-cyclic phosphate and 5'-OH ligase (RTCB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RTCB ( XM_017823464.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017678953.1 Lepidothrix coronata RNA 2',3'-cyclic phosphate and 5'-OH ligase (RTCB), mRNA.

Price & Availability↑

CloneID	OLe64226
Clone ID Related Accession (Same CDS sequence)	XM_017823464.1
Accession Version	XM_017823464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	tRNA-splicing ligase RtcB homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGCA GCTACAACGA TGAGCTGCAG TACCTGGACA AGATCGACAA GAACTGCTGG 
CGGATCAAGA AGGGCTTCGT GCCCAACATG CGTGTGGAGG GTGTTTTCTA CGTGAACGAC 
CCGCTGGAGA AGCTGATGTT CGAGGAGCTG CGCAACGCCT GCCGCGGAGG AGGTGCAGGT 
GGCTTCTTGC CTGCTATGAA ACAGATTGGG AACGTGGCTG CATTACCTGG TATTGTTCAC 
AGATCAATAG GCCTTCCAGA TGTCCATTCT GGCTATGGGT TTGCCATTGG CAACATGGCT 
GCCTTTGATA TGAGTGATCC AGAAGCAGTG GTTTCACCAG GTGGTGTAGG ATTTGACATC 
AACTGTGGTG TCCGCTTGCT GCGTACAAAT TTGGATGAAA GTGATGTGCA GCCTGTGAAA 
GAGCAGCTGG CCCAGGCTAT GTTTGACCAC ATTCCTGTTG GTGTTGGCTC CAAAGGTGTC 
ATCCCCATGA ATGCCAAGGA TTTGGAAGAG GCACTAGAGA TGGGTGTGGA CTGGTCTCTC 
CGGGAGGGCT ATGCCTGGGC AGAGGACAAG GAGCACTGTG AGGAATATGG AAGAATGCTG 
CAGGCTGATC CCAACAAAGT CTCCTCAAGA GCAAAGAAGA GGGGTCTGCC TCAGCTGGGG 
ACCCTGGGAG CTGGAAATCA TTACGCTGAG ATCCAGGTTG TGGATGACAT CTACAATGAA 
TATGCTGCCA AGAAGATGGG CATTGACCAC AAAGGGCAGG TGTGCGTGAT GATCCACAGT 
GGAAGCAGAG GTCTGGGCCA CCAGGTTGCC ACAGATGCAC TCGTGGCTAT GGAAAAAGCA 
ATGAAACGGG ACAAAATCAT AGTGAATGAT CGCCAGCTGG CGTGTGCCAG GATTGCCTCT 
GCTGAGGGAC AGGATTACTT GAAGGGAATG GCAGCTGCTG GGAACTATGC GTGGGTTAAC 
CGCTCTTCTA TGACCTTTCT AACAAGACAG GCCTTTGCCA AAGTCTTTAA CACAACCCCA 
GATGACCTTG ATATGCATGT TATCTATGAT GTGTCTCACA ACATTGCCAA AGTGGAGCAA 
CATGTAGTGG ATGGAAAAGA GCGGACTCTG CTGGTGCACA GAAAAGGATC AACCAGGGCC 
TTCCCTCCTC ACCATCCTCT TATTGCTGTT GATTATCAAC TGACAGGACA GCCTGTGTTG 
ATCGGCGGGA CTATGGGCAC CTGTAGCTAT GTCCTTACTG GCACAGAGCA AGGCATGACT 
GAGACCTTCG GAACTACCTG TCATGGAGCT GGTCGTGCAC TGTCTCGAGC CAAATCTCGA 
CGTAACTTGG ACTTCCAGGA TGTGTTGGAC AAGCTGGCTG ACATGGGCAT TGCCATCCGT 
GTTGCTTCTC CCAAACTGGT CATGGAAGAA GCACCAGAGT CTTACAAGAA CGTGACTGAT 
GTGGTTAACA CATGCCATGA AGCTGGCATC AGCAAGAAAG CCATTAAACT GAGACCTATT 
GCTGTTATAA AAGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017678953.1
CDS	49..1566
Translation	MSRSYNDELQYLDKIDKNCWRIKKGFVPNMRVEGVFYVNDPLEKLMFEELRNACRGGGAGGFLPAMKQIGNVAALPGIVHRSIGLPDVHSGYGFAIGNMAAFDMSDPEAVVSPGGVGFDINCGVRLLRTNLDESDVQPVKEQLAQAMFDHIPVGVGSKGVIPMNAKDLEEALEMGVDWSLREGYAWAEDKEHCEEYGRMLQADPNKVSSRAKKRGLPQLGTLGAGNHYAEIQVVDDIYNEYAAKKMGIDHKGQVCVMIHSGSRGLGHQVATDALVAMEKAMKRDKIIVNDRQLACARIASAEGQDYLKGMAAAGNYAWVNRSSMTFLTRQAFAKVFNTTPDDLDMHVIYDVSHNIAKVEQHVVDGKERTLLVHRKGSTRAFPPHHPLIAVDYQLTGQPVLIGGTMGTCSYVLTGTEQGMTETFGTTCHGAGRALSRAKSRRNLDFQDVLDKLADMGIAIRVASPKLVMEEAPESYKNVTDVVNTCHEAGISKKAIKLRPIAVIKG

Target ORF information:

RefSeq Version	XM_017823464.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata RNA 2',3'-cyclic phosphate and 5'-OH ligase (RTCB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017823464.1

ORF Insert Sequence:

ATGAGCCGCA GCTACAACGA TGAGCTGCAG TACCTGGACA AGATCGACAA GAACTGCTGG 
CGGATCAAGA AGGGCTTCGT GCCCAACATG CGTGTGGAGG GTGTTTTCTA CGTGAACGAC 
CCGCTGGAGA AGCTGATGTT CGAGGAGCTG CGCAACGCCT GCCGCGGAGG AGGTGCAGGT 
GGCTTCTTGC CTGCTATGAA ACAGATTGGG AACGTGGCTG CATTACCTGG TATTGTTCAC 
AGATCAATAG GCCTTCCAGA TGTCCATTCT GGCTATGGGT TTGCCATTGG CAACATGGCT 
GCCTTTGATA TGAGTGATCC AGAAGCAGTG GTTTCACCAG GTGGTGTAGG ATTTGACATC 
AACTGTGGTG TCCGCTTGCT GCGTACAAAT TTGGATGAAA GTGATGTGCA GCCTGTGAAA 
GAGCAGCTGG CCCAGGCTAT GTTTGACCAC ATTCCTGTTG GTGTTGGCTC CAAAGGTGTC 
ATCCCCATGA ATGCCAAGGA TTTGGAAGAG GCACTAGAGA TGGGTGTGGA CTGGTCTCTC 
CGGGAGGGCT ATGCCTGGGC AGAGGACAAG GAGCACTGTG AGGAATATGG AAGAATGCTG 
CAGGCTGATC CCAACAAAGT CTCCTCAAGA GCAAAGAAGA GGGGTCTGCC TCAGCTGGGG 
ACCCTGGGAG CTGGAAATCA TTACGCTGAG ATCCAGGTTG TGGATGACAT CTACAATGAA 
TATGCTGCCA AGAAGATGGG CATTGACCAC AAAGGGCAGG TGTGCGTGAT GATCCACAGT 
GGAAGCAGAG GTCTGGGCCA CCAGGTTGCC ACAGATGCAC TCGTGGCTAT GGAAAAAGCA 
ATGAAACGGG ACAAAATCAT AGTGAATGAT CGCCAGCTGG CGTGTGCCAG GATTGCCTCT 
GCTGAGGGAC AGGATTACTT GAAGGGAATG GCAGCTGCTG GGAACTATGC GTGGGTTAAC 
CGCTCTTCTA TGACCTTTCT AACAAGACAG GCCTTTGCCA AAGTCTTTAA CACAACCCCA 
GATGACCTTG ATATGCATGT TATCTATGAT GTGTCTCACA ACATTGCCAA AGTGGAGCAA 
CATGTAGTGG ATGGAAAAGA GCGGACTCTG CTGGTGCACA GAAAAGGATC AACCAGGGCC 
TTCCCTCCTC ACCATCCTCT TATTGCTGTT GATTATCAAC TGACAGGACA GCCTGTGTTG 
ATCGGCGGGA CTATGGGCAC CTGTAGCTAT GTCCTTACTG GCACAGAGCA AGGCATGACT 
GAGACCTTCG GAACTACCTG TCATGGAGCT GGTCGTGCAC TGTCTCGAGC CAAATCTCGA 
CGTAACTTGG ACTTCCAGGA TGTGTTGGAC AAGCTGGCTG ACATGGGCAT TGCCATCCGT 
GTTGCTTCTC CCAAACTGGT CATGGAAGAA GCACCAGAGT CTTACAAGAA CGTGACTGAT 
GTGGTTAACA CATGCCATGA AGCTGGCATC AGCAAGAAAG CCATTAAACT GAGACCTATT 
GCTGTTATAA AAGGCTAG