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CD3E cDNA ORF clone, Mus musculus(House mouse)

Gene Symbol CD3E
Entrez Gene ID 12501
Full Name CD3 antigen, epsilon polypeptide
Synonyms AI504783,CD3,CD3epsilon,T3e
General protein information
Preferred Names

CD3 antigen, epsilon polypeptide

Names

T-cell surface glycoprotein CD3 epsilon chain
T-cell surface antigen T3/Leu-4 epsilon chain

Gene Type protein-coding
Organism Mus musculus(House mouse)
Genome

9

9 A5.2|9 24.84 cM


mRNA and Protein(s)

mRNA Protein Name
XM_006509966.2 XP_006510029.1 T-cell surface glycoprotein CD3 epsilon chain isoform X1
NM_007648.4 NP_031674.1 T-cell surface glycoprotein CD3 epsilon chain precursor
XM_006509966.3 XP_006510029.1 T-cell surface glycoprotein CD3 epsilon chain isoform X1



Gallus gallus (chicken) CD3E NP_996787.1
Homo sapiens (human) CD3E NP_000724.1
Canis lupus familiaris (dog) CD3E NP_001003379.1
Mus musculus (house mouse) Cd3e NP_031674.1
Xenopus tropicalis (tropical clawed frog) cd3e XP_002932946.1
Pan troglodytes (chimpanzee) CD3E XP_001160742.1
Bos taurus (cattle) CD3E NP_776436.1
Rattus norvegicus (Norway rat) Cd3e NP_001101610.1
Macaca mulatta (rhesus monkey) CD3E XP_001097204.1


Related articles in PubMed

Protein Palmitoylation by ZDHHC13 Protects Skin against Microbial-Driven Dermatitis.
Chen LY, Yang-Yen HF, Tsai CC, Thio CL, Chuang HL, Yang LT, Shen LF, Song IW, Liu KM, Huang YT, Liu FT, Chang YJ, Chen YT, Yen JJ
The Journal of investigative dermatology137(4)894-904(2017 Apr)

Triggering the TCR Developmental Checkpoint Activates a Therapeutically Targetable Tumor Suppressive Pathway in T-cell Leukemia.
Trinquand A, Dos Santos NR, Tran Quang C, Rocchetti F, Zaniboni B, Belhocine M, Da Costa de Jesus C, Lhermitte L, Tesio M, Dussiot M, Cosset FL, Verhoeyen E, Pflumio F, Ifrah N, Dombret H, Spicuglia S, Chatenoud L, Gross DA, Hermine O, Macintyre E, Ghysdael J, Asnafi V
Cancer discovery6(9)972-85(2016 Sep)

Protein kinase D regulates positive selection of CD4
Ishikawa E, Kosako H, Yasuda T, Ohmuraya M, Araki K, Kurosaki T, Saito T, Yamasaki S
Nature communications712756(2016 Sep)

CD1d-restricted peripheral T cell lymphoma in mice and humans.
Bachy E, Urb M, Chandra S, Robinot R, Bricard G, de Bernard S, Traverse-Glehen A, Gazzo S, Blond O, Khurana A, Baseggio L, Heavican T, Ffrench M, Crispatzu G, Mondière P, Schrader A, Taillardet M, Thaunat O, Martin N, Dalle S, Le Garff-Tavernier M, Salles G, Lachuer J, Hermine O, Asnafi V, Roussel M, Lamy T, Herling M, Iqbal J, Buffat L, Marche PN, Gaulard P, Kronenberg M, Defrance T, Genestier L
The Journal of experimental medicine213(5)841-57(2016 May)

Pulmonary neuroendocrine cells function as airway sensors to control lung immune response.
Branchfield K, Nantie L, Verheyden JM, Sui P, Wienhold MD, Sun X
Science (New York, N.Y.)351(6274)707-10(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data show that CD3epsilon chain couples to Cbl via simultaneous binding of both proteins to Numb, thus mediating TCR degradation
Title: CD3ε recruits Numb to promote TCR degradation.

IL-17-producing CD3(bright) gammadelta T cells responded promptly and strongly to pneumococcal infection and during skin inflammation.
Title: CD3bright signals on γδ T cells identify IL-17A-producing Vγ6Vδ1+ T cells.

these results indicate that membrane association of the CD3epsilon signaling domain is required for optimal thymocyte development and peripheral T cell function.
Title: Membrane association of the CD3ε signaling domain is required for optimal T cell development and function.

these results suggest that CD3zeta can be degraded by two pathways: SLAP/c-Cbl, which targets internalized cell surface CD3zeta dependent on TCR signaling, and LAPTM5, which targets intracellular CD3zeta independent of TCR signaling.
Title: LAPTM5 promotes lysosomal degradation of intracellular CD3ζ but not of cell surface CD3ζ.

These results suggest that proteasome-mediated degradation is involved in hypophosphorylated LAT and PLCgamma1 in Dow2-induced anergic T cells. The novel CD3-specific Ab, Dow2, may provide us with a unique tool for inducing immunosuppression
Title: Novel CD3-specific antibody induces immunosuppression via impaired phosphorylation of LAT and PLCγ1 following T-cell stimulation.

The following CD3E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD3E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OMu20096 NM_007648.4
Latest version!
Mus musculus CD3 antigen, epsilon polypeptide (Cd3e), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
OMu20096 XM_006509966.3
Latest version!
Mus musculus CD3 antigen, epsilon polypeptide (Cd3e), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
View Old Accession Versions >>
OMu20096 XM_006509966.2 Mus musculus CD3 antigen, epsilon polypeptide (Cd3e), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OMu20096
Clone ID Related Accession (Same CDS sequence) XM_006509966.2 , NM_007648.4 , XM_006509966.3
Accession Version NM_007648.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 570bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Mus musculus(House mouse)
Product T-cell surface glycoprotein CD3 epsilon chain precursor
Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC122305.4. On Oct 19, 2007 this sequence version replaced NM_007648.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J02990.1, AI323257.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
ATGCGGTGGA ACACTTTCTG GGGCATCCTG TGCCTCAGCC TCCTAGCTGT TGGCACTTGC 
CAGGACGATG CCGAGAACAT TGAATACAAA GTCTCCATCT CAGGAACCAG TGTAGAGTTG
ACGTGCCCTC TAGACAGTGA CGAGAACTTA AAATGGGAAA AAAATGGCCA AGAGCTGCCT
CAGAAGCATG ATAAGCACCT GGTGCTCCAG GATTTCTCGG AAGTCGAGGA CAGTGGCTAC
TACGTCTGCT ACACACCAGC CTCAAATAAA AACACGTACT TGTACCTGAA AGCTCGAGTG
TGTGAGTACT GTGTGGAGGT GGACCTGACA GCAGTAGCCA TAATCATCAT TGTTGACATC
TGTATCACTC TGGGCTTGCT GATGGTCATT TATTACTGGA GCAAGAATAG GAAGGCCAAG
GCCAAGCCTG TGACCCGAGG AACCGGTGCT GGTAGCAGGC CCAGAGGGCA AAACAAGGAG
CGGCCACCAC CTGTTCCCAA CCCAGACTAT GAGCCCATCC GCAAAGGCCA GCGGGACCTG
TATTCTGGCC TGAATCAGAG AGCAGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_031674.1
CDS79..648
Misc Feature(1)58..60
Misc Feature(2)163..387
Misc Feature(3)181..>327
Misc Feature(4)403..480
Misc Feature(5)577..639
Misc Feature(6)586..588
Misc Feature(7)619..621
Exon (1)1..25
Exon (2)26..127
Exon (3)128..145
Exon (4)146..160
Exon (5)161..376
Exon (6)377..544
Exon (7)545..591
Exon (8)592..1436
Translation

Target ORF information:

RefSeq Version NM_007648.4
Organism Mus musculus(House mouse)
Definition Mus musculus CD3 antigen, epsilon polypeptide (Cd3e), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_007648.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGCGGTGGA ACACTTTCTG GGGCATCCTG TGCCTCAGCC TCCTAGCTGT TGGCACTTGC 
CAGGACGATG CCGAGAACAT TGAATACAAA GTCTCCATCT CAGGAACCAG TGTAGAGTTG
ACGTGCCCTC TAGACAGTGA CGAGAACTTA AAATGGGAAA AAAATGGCCA AGAGCTGCCT
CAGAAGCATG ATAAGCACCT GGTGCTCCAG GATTTCTCGG AAGTCGAGGA CAGTGGCTAC
TACGTCTGCT ACACACCAGC CTCAAATAAA AACACGTACT TGTACCTGAA AGCTCGAGTG
TGTGAGTACT GTGTGGAGGT GGACCTGACA GCAGTAGCCA TAATCATCAT TGTTGACATC
TGTATCACTC TGGGCTTGCT GATGGTCATT TATTACTGGA GCAAGAATAG GAAGGCCAAG
GCCAAGCCTG TGACCCGAGG AACCGGTGCT GGTAGCAGGC CCAGAGGGCA AAACAAGGAG
CGGCCACCAC CTGTTCCCAA CCCAGACTAT GAGCCCATCC GCAAAGGCCA GCGGGACCTG
TATTCTGGCC TGAATCAGAG AGCAGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu20096
Clone ID Related Accession (Same CDS sequence) XM_006509966.2 , NM_007648.4 , XM_006509966.3
Accession Version XM_006509966.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 570bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-02-09
Organism Mus musculus(House mouse)
Product T-cell surface glycoprotein CD3 epsilon chain isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
ATGCGGTGGA ACACTTTCTG GGGCATCCTG TGCCTCAGCC TCCTAGCTGT TGGCACTTGC 
CAGGACGATG CCGAGAACAT TGAATACAAA GTCTCCATCT CAGGAACCAG TGTAGAGTTG
ACGTGCCCTC TAGACAGTGA CGAGAACTTA AAATGGGAAA AAAATGGCCA AGAGCTGCCT
CAGAAGCATG ATAAGCACCT GGTGCTCCAG GATTTCTCGG AAGTCGAGGA CAGTGGCTAC
TACGTCTGCT ACACACCAGC CTCAAATAAA AACACGTACT TGTACCTGAA AGCTCGAGTG
TGTGAGTACT GTGTGGAGGT GGACCTGACA GCAGTAGCCA TAATCATCAT TGTTGACATC
TGTATCACTC TGGGCTTGCT GATGGTCATT TATTACTGGA GCAAGAATAG GAAGGCCAAG
GCCAAGCCTG TGACCCGAGG AACCGGTGCT GGTAGCAGGC CCAGAGGGCA AAACAAGGAG
CGGCCACCAC CTGTTCCCAA CCCAGACTAT GAGCCCATCC GCAAAGGCCA GCGGGACCTG
TATTCTGGCC TGAATCAGAG AGCAGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006510029.1
Translation

Target ORF information:

RefSeq Version XM_006509966.2
Organism Mus musculus(House mouse)
Definition Mus musculus CD3 antigen, epsilon polypeptide (Cd3e), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006509966.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGCGGTGGA ACACTTTCTG GGGCATCCTG TGCCTCAGCC TCCTAGCTGT TGGCACTTGC 
CAGGACGATG CCGAGAACAT TGAATACAAA GTCTCCATCT CAGGAACCAG TGTAGAGTTG
ACGTGCCCTC TAGACAGTGA CGAGAACTTA AAATGGGAAA AAAATGGCCA AGAGCTGCCT
CAGAAGCATG ATAAGCACCT GGTGCTCCAG GATTTCTCGG AAGTCGAGGA CAGTGGCTAC
TACGTCTGCT ACACACCAGC CTCAAATAAA AACACGTACT TGTACCTGAA AGCTCGAGTG
TGTGAGTACT GTGTGGAGGT GGACCTGACA GCAGTAGCCA TAATCATCAT TGTTGACATC
TGTATCACTC TGGGCTTGCT GATGGTCATT TATTACTGGA GCAAGAATAG GAAGGCCAAG
GCCAAGCCTG TGACCCGAGG AACCGGTGCT GGTAGCAGGC CCAGAGGGCA AAACAAGGAG
CGGCCACCAC CTGTTCCCAA CCCAGACTAT GAGCCCATCC GCAAAGGCCA GCGGGACCTG
TATTCTGGCC TGAATCAGAG AGCAGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu20096
Clone ID Related Accession (Same CDS sequence) XM_006509966.2 , NM_007648.4 , XM_006509966.3
Accession Version XM_006509966.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 570bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-22
Organism Mus musculus(House mouse)
Product T-cell surface glycoprotein CD3 epsilon chain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509966.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
ATGCGGTGGA ACACTTTCTG GGGCATCCTG TGCCTCAGCC TCCTAGCTGT TGGCACTTGC 
CAGGACGATG CCGAGAACAT TGAATACAAA GTCTCCATCT CAGGAACCAG TGTAGAGTTG
ACGTGCCCTC TAGACAGTGA CGAGAACTTA AAATGGGAAA AAAATGGCCA AGAGCTGCCT
CAGAAGCATG ATAAGCACCT GGTGCTCCAG GATTTCTCGG AAGTCGAGGA CAGTGGCTAC
TACGTCTGCT ACACACCAGC CTCAAATAAA AACACGTACT TGTACCTGAA AGCTCGAGTG
TGTGAGTACT GTGTGGAGGT GGACCTGACA GCAGTAGCCA TAATCATCAT TGTTGACATC
TGTATCACTC TGGGCTTGCT GATGGTCATT TATTACTGGA GCAAGAATAG GAAGGCCAAG
GCCAAGCCTG TGACCCGAGG AACCGGTGCT GGTAGCAGGC CCAGAGGGCA AAACAAGGAG
CGGCCACCAC CTGTTCCCAA CCCAGACTAT GAGCCCATCC GCAAAGGCCA GCGGGACCTG
TATTCTGGCC TGAATCAGAG AGCAGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006510029.1
CDS150..719
Misc Feature(1)234..458
Misc Feature(2)252..>398
Misc Feature(3)648..710
Translation

Target ORF information:

RefSeq Version XM_006509966.3
Organism Mus musculus(House mouse)
Definition Mus musculus CD3 antigen, epsilon polypeptide (Cd3e), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006509966.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGCGGTGGA ACACTTTCTG GGGCATCCTG TGCCTCAGCC TCCTAGCTGT TGGCACTTGC 
CAGGACGATG CCGAGAACAT TGAATACAAA GTCTCCATCT CAGGAACCAG TGTAGAGTTG
ACGTGCCCTC TAGACAGTGA CGAGAACTTA AAATGGGAAA AAAATGGCCA AGAGCTGCCT
CAGAAGCATG ATAAGCACCT GGTGCTCCAG GATTTCTCGG AAGTCGAGGA CAGTGGCTAC
TACGTCTGCT ACACACCAGC CTCAAATAAA AACACGTACT TGTACCTGAA AGCTCGAGTG
TGTGAGTACT GTGTGGAGGT GGACCTGACA GCAGTAGCCA TAATCATCAT TGTTGACATC
TGTATCACTC TGGGCTTGCT GATGGTCATT TATTACTGGA GCAAGAATAG GAAGGCCAAG
GCCAAGCCTG TGACCCGAGG AACCGGTGCT GGTAGCAGGC CCAGAGGGCA AAACAAGGAG
CGGCCACCAC CTGTTCCCAA CCCAGACTAT GAGCCCATCC GCAAAGGCCA GCGGGACCTG
TATTCTGGCC TGAATCAGAG AGCAGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Protein Palmitoylation by ZDHHC13 Protects Skin against Microbial-Driven Dermatitis.
The Journal of investigative dermatology137(4)894-904(2017 Apr)
Chen LY,Yang-Yen HF,Tsai CC,Thio CL,Chuang HL,Yang LT,Shen LF,Song IW,Liu KM,Huang YT,Liu FT,Chang YJ,Chen YT,Yen JJ


book

Triggering the TCR Developmental Checkpoint Activates a Therapeutically Targetable Tumor Suppressive Pathway in T-cell Leukemia.
Cancer discovery6(9)972-85(2016 Sep)
Trinquand A,Dos Santos NR,Tran Quang C,Rocchetti F,Zaniboni B,Belhocine M,Da Costa de Jesus C,Lhermitte L,Tesio M,Dussiot M,Cosset FL,Verhoeyen E,Pflumio F,Ifrah N,Dombret H,Spicuglia S,Chatenoud L,Gross DA,Hermine O,Macintyre E,Ghysdael J,Asnafi V


book

Protein kinase D regulates positive selection of CD4
Nature communications712756(2016 Sep)
Ishikawa E,Kosako H,Yasuda T,Ohmuraya M,Araki K,Kurosaki T,Saito T,Yamasaki S


book

CD1d-restricted peripheral T cell lymphoma in mice and humans.
The Journal of experimental medicine213(5)841-57(2016 May)
Bachy E,Urb M,Chandra S,Robinot R,Bricard G,de Bernard S,Traverse-Glehen A,Gazzo S,Blond O,Khurana A,Baseggio L,Heavican T,Ffrench M,Crispatzu G,Mondière P,Schrader A,Taillardet M,Thaunat O,Martin N,Dalle S,Le Garff-Tavernier M,Salles G,Lachuer J,Hermine O,Asnafi V,Roussel M,Lamy T,Herling M,Iqbal J,Buffat L,Marche PN,Gaulard P,Kronenberg M,Defrance T,Genestier L


book

Pulmonary neuroendocrine cells function as airway sensors to control lung immune response.
Science (New York, N.Y.)351(6274)707-10(2016 Feb)
Branchfield K,Nantie L,Verheyden JM,Sui P,Wienhold MD,Sun X


 
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