Ncan cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ncan
Entrez Gene ID	13004
Full Name	neurocan
Synonyms	C230035B04,Cspg3,Cspg3-rs,Tgfbit
General protein information	Preferred Names neurocan Names neurocan core protein TGFbeta-induced transcript, brain chondroitin sulfate proteoglycan 3, related sequence
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 B3.3\|8 34.15 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_007789.3	NP_031815.2	neurocan core protein precursor
XM_006509543.3	XP_006509606.1	neurocan core protein isoform X1
XM_006509543.4	XP_006509606.1	neurocan core protein isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NCAN	NP_004377.2
Mus musculus (house mouse)	Ncan	NP_031815.2
Canis lupus familiaris (dog)	NCAN	XP_541924.3
Bos taurus (cattle)	NCAN	NP_001180011.1
Rattus norvegicus (Norway rat)	Ncan	NP_113841.2
Pan troglodytes (chimpanzee)	NCAN	NP_001029342.1
	NCAN	XP_002808228.1
Rattus norvegicus (Norway rat)	LOC102550316	XP_006253042.1

Gene Ontology
Bibliography

Related articles in PubMed

Intrinsic cellular and molecular properties of in vivo hippocampal synaptic plasticity are altered in the absence of key synaptic matrix molecules.
Jansen S, Gottschling C, Faissner A, Manahan-Vaughan D
Hippocampus27(8)920-933(2017 Aug)

?-amyloid increases neurocan expression through regulating Sox9 in astrocytes: A potential relationship between Sox9 and chondroitin sulfate proteoglycans in Alzheimer's disease.
Yan H, Zhu X, Xie J, Zhao Y, Liu X
Brain research1646377-383(2016 09)

Muscleblind-like 1 is required for normal heart valve development in vivo.
Coram RJ, Stillwagon SJ, Guggilam A, Jenkins MW, Swanson MS, Ladd AN
BMC developmental biology1536(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study shows the viability and proliferation of astrocytes are significantly increased by beta-amyloid in a dose dependent manner. Moreover, beta-amyloid may effectively up-regulate the neurocan expression via regulating Sox9.
Title: ?-amyloid increases neurocan expression through regulating Sox9 in astrocytes: A potential relationship between Sox9 and chondroitin sulfate proteoglycans in Alzheimer's disease.

This study provided evidence that the neurocan modulates synaptogenesis and long-term synapse stability. The mutant neurons display reduced frequencies of mEPSCs and mIPSCs.
Title: Primary hippocampal neurons, which lack four crucial extracellular matrix molecules, display abnormalities of synaptic structure and function and severe deficits in perineuronal net formation.

Traumatic brain injury results in an increase in the chondroitin glycosaminoglycans (CSPGs) neurocan and NG2 expression in a tight band surrounding the injury core.
Title: Alterations in sulfated chondroitin glycosaminoglycans following controlled cortical impact injury in mice.

Ncan -/- mice showed behavioral abnormalities that were strikingly similar to those of the human mania phenotype.
Title: Studies in humans and mice implicate neurocan in the etiology of mania.

neurocan contributes to the molecular heterogeneity of the perinodal matrix, and its nodal expression is dependent on Bral1.
Title: Neurocan contributes to the molecular heterogeneity of the perinodal ECM.

The following Ncan gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ncan cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00807	NM_007789.3 Latest version!	Mus musculus neurocan (Ncan), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$741.30 $1059.00
OMu40921	XM_006509543.4 Latest version!	Mus musculus neurocan (Ncan), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OMu40921	XM_006509543.3	Mus musculus neurocan (Ncan), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ncan ( XM_006509543.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509606.1 Mus musculus neurocan (Ncan), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu40921
Clone ID Related Accession (Same CDS sequence)	XM_006509543.3 , XM_006509543.4
Accession Version	XM_006509543.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurocan core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509543.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT CAGGGTCAGG ACCCGTCCGT GCTGCCCTGG CAGAGCTAGT GGCCCTGCCC 
TGCTTCTTTA CCCTGCAACC ACGGCTAAGC TCCCTGCGAG ACATTCCTCG GATCAAGTGG 
ACTAAGGTTC AGACTGCATC AGGCCAGCGA CAGGATTTGC CAATCTTGGT GGCCAAAGAC 
AACGTGGTGC GTGTGGCCAA GGGCTGGCAG GGACGGGTGT CATTGCCTGC TTATCCCCGG 
CACAGAGCCA ATGCTACCCT TCTGCTGGGG CCACTTCGAG CAAGCGACTC TGGGCTGTAT 
CGCTGCCAAG TGGTAAAGGG TATCGAAGAT GAGCAGGACC TGGTAACCCT GGAAGTGACA 
GGCGTCGTGT TCCATTATCG GGCGGCCCGG GACCGCTATG CGCTGACCTT CGCTGAGGCC 
CAGGAGGCTT GTCGCCTAAG CTCTGCTACC ATCGCTGCCC CACGGCACCT GCAGGCTGCC 
TTTGAAGATG GCTTTGACAA CTGCGACGCG GGCTGGCTCT CAGACCGCAC GGTTCGGTAC 
CCGATCACTC AGTCGCGTCC TGGTTGCTAT GGTGACCGCA GCAGTCTCCC GGGTGTTCGG 
AGCTACGGGA GACGCGACCC GCAGGAACTC TACGATGTCT ACTGCTTTGC CCGCGAGCTA 
GGGGGTGAAG TCTTTTACGT GGGCCCGGCC CGCCGACTGA CCCTGGCGGG CGCGCGGGCA 
CAATGTCAGC GGCAGGGTGC AGCGCTGGCC TCGGTGGGGC AGTTGCACCT GGCCTGGCAC 
GAGGGCCTGG ACCAGTGCGA CCCGGGCTGG CTGGCAGACG GCAGCGTGCG CTACCCCATC 
CAGACTCCGC GCCGGCGTTG CGGGGGCCCC GCCCCAGGTG TGCGCACTGT GTACCGCTTC 
GCCAACCGCA CCGGCTTTCC TGCGCCAGGA GCACGCTTCG ACGCCTACTG CTTCCGAGCT 
CATCACCATA CAGCACAACA TGGAGATTCT GAGATCCCCT CATCTGGAGA TGAGGGGGAG 
ATTGTGTCAG CAGAGGGGCC TCCAGGCCGA GAACTAAAGC CCAGCTTGGG GGAACAGGAG 
GTGATAGCAC CTGACTTCCA GGAACCTCTC ATGTCCAGTG GAGAAGGCGA ACCCCCAGAC 
TTGACTTGGA CACAAGCACC TGAGGAGACC CTTGGTTCTA CTCCAGGGGG TCCCACGCTG 
GCCTCATGGC CCTCTTCAGA AAAGTGGCTC TTTACGGGTG CCCCAAGCTC CATGGGTGTC 
TCCAGTCCCA GCGACATGGG AGTAGATATG GAAGCGACAA CACCCTTGGG CACACAGGTA 
GCACCCACCC CCACGATGAG GAGGGGCCGC TTTAAAGGGT TGAATGGTCG ACACTTCCAG 
CAGCAGGGCC CAGAAGACCA GCTGCCTGAG GTAGCAGAGC CCAGTGCCCA GCCTCCCACC 
CTGGGAGCTA CTGCCAACCA CATGAGGCCT TCTGCAGCCA CAGAGGCTTC AGAGAGTGAC 
CAGAGCCACA GTCCTTGGGC CATTCTGACC AACGAAGTGG ATGAGCCAGG AGCAGGCTCT 
CTTGGCAGCA GGAGTCTCCC AGAGTCCCTG ATGTGGTCCC CGTCGTTGAT CTCACCCAGT 
GTCCCAAGCA CCGACAGTAC TCCTAGCGCG AAGCCAGGGG CAGCCGAGGC ACCCAGTGTG 
AAGTCAGCCA TCCCCCACCT GCCCCGGCTG CCCTCAGAGC CCCCTGCTCC CTCTCCCGGG 
CCCTCAGAGG CCCTAAGTGC TGTCTCCCTC CAGGCATCCT CTGCTGATGG CTCCCCTGAC 
TTCCCCATTG TAGCCATGCT TCGAGCCCCC AAACTGTGGC TTCTGCCACG CTCTACACTT 
GTCCCCAATA TGACCCCAGT CCCACTGTCC CCAGCTTCTC CACTTCCCTC CTGGGTCCCA 
GAAGAACAGG CTGTCAGGCC TGTCAGCCTT GGAGCGGAAG ACCTCGAAAC CCCATTTCAG 
ACCACCATAG CTGCCCCAGT TGAAGCCAGC CACAGATCCC CTGATGCAGA TTCTATAGAA 
ATCGAGGGGA CCAGCTCCAT GCGGGCTACA AAGCACCCCA TCTCTGGCCC ATGGGCTTCT 
TTGGACTCTA GTAATGTGAC GATGAATCCT GTCCCTTCTG ATGCTGGCAT CCTAGGGACT 
GAGTCTGGGG TCTTGGACTT ACCAGGGAGT CCCACATCAG GCGGACAGGC CACTGTGGAG 
AAGGTGCTGG CCACCTGGCT ACCACTGCCT GGCCAAGGAC TAGACCCTGG CTCCCAGTCC 
ACACCCATGG AAGCCCATGG GGTTGCCGTG AGCATGGAAC CTACAGTGGC TTTGGAGGGA 
GGTGCCACCG AGGGCCCTAT GGAGGCCACC AGGGAGGTGG TCCCCAGCAC TGCTGATGCC 
ACTTGGGAGT CTGAATCCAG AAGTGCCATT TCTAGCACCC ACATAGCTGT GACCATGGCT 
AGGGCTCAGG GCATGCCCAC ACTGACCTCT ACAAGCTCCG AAGGCCACCC AGAGCCTAAG 
GGCCAGATGG TGGCCCAGGA GTCACTGGAG CCTCTCAACA CTCTGCCTTC GCACCCCTGG 
TCATCCCTGG TGGTCCCCAT GGATGAAGTG GCCTCAGTTT CCTCAGGGGA ACCCACAGGA 
CTGTGGGACA TACCCAGCAC TCTGATACCT GTATCCTTGG GCTTGGATGA ATCAGACCTG 
AATGTTGTGG CTGAGAGTCC AAGCGTGAAG GGCTTCTGGG AAGAGGTGGC CAGCGGGCAG 
GAAGATCCCA CAGATCCCTG CGAGAACAAC CCCTGCCTGC ATGGGGGCAC CTGCCACACC 
AACGGCACCG TGTATGGCTG TAGTTGTGAC CAGGGCTATG CTGGGGAGAA TTGTGAAATT 
GACATTGATG ACTGCTTATG CAGCCCTTGC GAGAATGGGG GCACCTGCAT CGACGAGGTG 
AATGGTTTCA TCTGCCTCTG CCTCCCCAGC TATGGGGGCA GCCTGTGTGA GAAGGACACA 
GAAGGCTGCG ACCGTGGCTG GCACAAATTC CAGGGACACT GCTACCGGTA CTTTGCCCAT 
CGCCGGGCCT GGGAGGATGC AGAGAGAGAT TGCAGGCGCC GAGCTGGTCA CCTGACAAGC 
GTCCATTCGC CAGAAGAGCA CAAGTTCATT AACAGTTTTG GACATGAGAA TTCGTGGATC 
GGCCTGAATG ACCGGACAGT AGAGAGGGAC TTCCAGTGGA CAGACAACAC AGGACTGCAA 
TATGAGAACT GGAGGGAGAA GCAGCCGGAT AATTTCTTCG CAGGTGGGGA GGATTGTGTG 
GTGATGGTGG CACATGAGAG TGGGCGCTGG AATGATGTCC CCTGCAACTA CAACCTACCC 
TATGTCTGCA AGAAGGGTAC AGTGCTGTGT GGTCCCCCTC CAGCAGTGGA GAATGCCTCT 
CTTGTTGGTG TGCGCAAGAT CAAGTACAAT GTCCATGCCA CTGTGCGATA CCAGTGTGAT 
GAAGGATTCT CCCAGCACCG AGTGGCCACT ATCCGCTGCC GTAACAATGG GAAGTGGGAC 
CGGCCTCAGA TCATGTGCAT CAAACCCAGG CGGTCACATC GGATGCGTAG ACACCACCAC 
CATCCACACC GACATCACAA GCCTCGCAAG GAGCACAGAA AACACAAGAG ACACCCAGCG 
GAAGACTGGG AGAAAGACGA AGGGGATTTC TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509606.1
CDS	178..3873
Translation	MLKSGSGPVRAALAELVALPCFFTLQPRLSSLRDIPRIKWTKVQTASGQRQDLPILVAKDNVVRVAKGWQGRVSLPAYPRHRANATLLLGPLRASDSGLYRCQVVKGIEDEQDLVTLEVTGVVFHYRAARDRYALTFAEAQEACRLSSATIAAPRHLQAAFEDGFDNCDAGWLSDRTVRYPITQSRPGCYGDRSSLPGVRSYGRRDPQELYDVYCFARELGGEVFYVGPARRLTLAGARAQCQRQGAALASVGQLHLAWHEGLDQCDPGWLADGSVRYPIQTPRRRCGGPAPGVRTVYRFANRTGFPAPGARFDAYCFRAHHHTAQHGDSEIPSSGDEGEIVSAEGPPGRELKPSLGEQEVIAPDFQEPLMSSGEGEPPDLTWTQAPEETLGSTPGGPTLASWPSSEKWLFTGAPSSMGVSSPSDMGVDMEATTPLGTQVAPTPTMRRGRFKGLNGRHFQQQGPEDQLPEVAEPSAQPPTLGATANHMRPSAATEASESDQSHSPWAILTNEVDEPGAGSLGSRSLPESLMWSPSLISPSVPSTDSTPSAKPGAAEAPSVKSAIPHLPRLPSEPPAPSPGPSEALSAVSLQASSADGSPDFPIVAMLRAPKLWLLPRSTLVPNMTPVPLSPASPLPSWVPEEQAVRPVSLGAEDLETPFQTTIAAPVEASHRSPDADSIEIEGTSSMRATKHPISGPWASLDSSNVTMNPVPSDAGILGTESGVLDLPGSPTSGGQATVEKVLATWLPLPGQGLDPGSQSTPMEAHGVAVSMEPTVALEGGATEGPMEATREVVPSTADATWESESRSAISSTHIAVTMARAQGMPTLTSTSSEGHPEPKGQMVAQESLEPLNTLPSHPWSSLVVPMDEVASVSSGEPTGLWDIPSTLIPVSLGLDESDLNVVAESPSVKGFWEEVASGQEDPTDPCENNPCLHGGTCHTNGTVYGCSCDQGYAGENCEIDIDDCLCSPCENGGTCIDEVNGFICLCLPSYGGSLCEKDTEGCDRGWHKFQGHCYRYFAHRRAWEDAERDCRRRAGHLTSVHSPEEHKFINSFGHENSWIGLNDRTVERDFQWTDNTGLQYENWREKQPDNFFAGGEDCVVMVAHESGRWNDVPCNYNLPYVCKKGTVLCGPPPAVENASLVGVRKIKYNVHATVRYQCDEGFSQHRVATIRCRNNGKWDRPQIMCIKPRRSHRMRRHHHHPHRHHKPRKEHRKHKRHPAEDWEKDEGDFC

Target ORF information:

RefSeq Version	XM_006509543.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurocan (Ncan), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509543.3

ORF Insert Sequence:

ATGCTGAAGT CAGGGTCAGG ACCCGTCCGT GCTGCCCTGG CAGAGCTAGT GGCCCTGCCC 
TGCTTCTTTA CCCTGCAACC ACGGCTAAGC TCCCTGCGAG ACATTCCTCG GATCAAGTGG 
ACTAAGGTTC AGACTGCATC AGGCCAGCGA CAGGATTTGC CAATCTTGGT GGCCAAAGAC 
AACGTGGTGC GTGTGGCCAA GGGCTGGCAG GGACGGGTGT CATTGCCTGC TTATCCCCGG 
CACAGAGCCA ATGCTACCCT TCTGCTGGGG CCACTTCGAG CAAGCGACTC TGGGCTGTAT 
CGCTGCCAAG TGGTAAAGGG TATCGAAGAT GAGCAGGACC TGGTAACCCT GGAAGTGACA 
GGCGTCGTGT TCCATTATCG GGCGGCCCGG GACCGCTATG CGCTGACCTT CGCTGAGGCC 
CAGGAGGCTT GTCGCCTAAG CTCTGCTACC ATCGCTGCCC CACGGCACCT GCAGGCTGCC 
TTTGAAGATG GCTTTGACAA CTGCGACGCG GGCTGGCTCT CAGACCGCAC GGTTCGGTAC 
CCGATCACTC AGTCGCGTCC TGGTTGCTAT GGTGACCGCA GCAGTCTCCC GGGTGTTCGG 
AGCTACGGGA GACGCGACCC GCAGGAACTC TACGATGTCT ACTGCTTTGC CCGCGAGCTA 
GGGGGTGAAG TCTTTTACGT GGGCCCGGCC CGCCGACTGA CCCTGGCGGG CGCGCGGGCA 
CAATGTCAGC GGCAGGGTGC AGCGCTGGCC TCGGTGGGGC AGTTGCACCT GGCCTGGCAC 
GAGGGCCTGG ACCAGTGCGA CCCGGGCTGG CTGGCAGACG GCAGCGTGCG CTACCCCATC 
CAGACTCCGC GCCGGCGTTG CGGGGGCCCC GCCCCAGGTG TGCGCACTGT GTACCGCTTC 
GCCAACCGCA CCGGCTTTCC TGCGCCAGGA GCACGCTTCG ACGCCTACTG CTTCCGAGCT 
CATCACCATA CAGCACAACA TGGAGATTCT GAGATCCCCT CATCTGGAGA TGAGGGGGAG 
ATTGTGTCAG CAGAGGGGCC TCCAGGCCGA GAACTAAAGC CCAGCTTGGG GGAACAGGAG 
GTGATAGCAC CTGACTTCCA GGAACCTCTC ATGTCCAGTG GAGAAGGCGA ACCCCCAGAC 
TTGACTTGGA CACAAGCACC TGAGGAGACC CTTGGTTCTA CTCCAGGGGG TCCCACGCTG 
GCCTCATGGC CCTCTTCAGA AAAGTGGCTC TTTACGGGTG CCCCAAGCTC CATGGGTGTC 
TCCAGTCCCA GCGACATGGG AGTAGATATG GAAGCGACAA CACCCTTGGG CACACAGGTA 
GCACCCACCC CCACGATGAG GAGGGGCCGC TTTAAAGGGT TGAATGGTCG ACACTTCCAG 
CAGCAGGGCC CAGAAGACCA GCTGCCTGAG GTAGCAGAGC CCAGTGCCCA GCCTCCCACC 
CTGGGAGCTA CTGCCAACCA CATGAGGCCT TCTGCAGCCA CAGAGGCTTC AGAGAGTGAC 
CAGAGCCACA GTCCTTGGGC CATTCTGACC AACGAAGTGG ATGAGCCAGG AGCAGGCTCT 
CTTGGCAGCA GGAGTCTCCC AGAGTCCCTG ATGTGGTCCC CGTCGTTGAT CTCACCCAGT 
GTCCCAAGCA CCGACAGTAC TCCTAGCGCG AAGCCAGGGG CAGCCGAGGC ACCCAGTGTG 
AAGTCAGCCA TCCCCCACCT GCCCCGGCTG CCCTCAGAGC CCCCTGCTCC CTCTCCCGGG 
CCCTCAGAGG CCCTAAGTGC TGTCTCCCTC CAGGCATCCT CTGCTGATGG CTCCCCTGAC 
TTCCCCATTG TAGCCATGCT TCGAGCCCCC AAACTGTGGC TTCTGCCACG CTCTACACTT 
GTCCCCAATA TGACCCCAGT CCCACTGTCC CCAGCTTCTC CACTTCCCTC CTGGGTCCCA 
GAAGAACAGG CTGTCAGGCC TGTCAGCCTT GGAGCGGAAG ACCTCGAAAC CCCATTTCAG 
ACCACCATAG CTGCCCCAGT TGAAGCCAGC CACAGATCCC CTGATGCAGA TTCTATAGAA 
ATCGAGGGGA CCAGCTCCAT GCGGGCTACA AAGCACCCCA TCTCTGGCCC ATGGGCTTCT 
TTGGACTCTA GTAATGTGAC GATGAATCCT GTCCCTTCTG ATGCTGGCAT CCTAGGGACT 
GAGTCTGGGG TCTTGGACTT ACCAGGGAGT CCCACATCAG GCGGACAGGC CACTGTGGAG 
AAGGTGCTGG CCACCTGGCT ACCACTGCCT GGCCAAGGAC TAGACCCTGG CTCCCAGTCC 
ACACCCATGG AAGCCCATGG GGTTGCCGTG AGCATGGAAC CTACAGTGGC TTTGGAGGGA 
GGTGCCACCG AGGGCCCTAT GGAGGCCACC AGGGAGGTGG TCCCCAGCAC TGCTGATGCC 
ACTTGGGAGT CTGAATCCAG AAGTGCCATT TCTAGCACCC ACATAGCTGT GACCATGGCT 
AGGGCTCAGG GCATGCCCAC ACTGACCTCT ACAAGCTCCG AAGGCCACCC AGAGCCTAAG 
GGCCAGATGG TGGCCCAGGA GTCACTGGAG CCTCTCAACA CTCTGCCTTC GCACCCCTGG 
TCATCCCTGG TGGTCCCCAT GGATGAAGTG GCCTCAGTTT CCTCAGGGGA ACCCACAGGA 
CTGTGGGACA TACCCAGCAC TCTGATACCT GTATCCTTGG GCTTGGATGA ATCAGACCTG 
AATGTTGTGG CTGAGAGTCC AAGCGTGAAG GGCTTCTGGG AAGAGGTGGC CAGCGGGCAG 
GAAGATCCCA CAGATCCCTG CGAGAACAAC CCCTGCCTGC ATGGGGGCAC CTGCCACACC 
AACGGCACCG TGTATGGCTG TAGTTGTGAC CAGGGCTATG CTGGGGAGAA TTGTGAAATT 
GACATTGATG ACTGCTTATG CAGCCCTTGC GAGAATGGGG GCACCTGCAT CGACGAGGTG 
AATGGTTTCA TCTGCCTCTG CCTCCCCAGC TATGGGGGCA GCCTGTGTGA GAAGGACACA 
GAAGGCTGCG ACCGTGGCTG GCACAAATTC CAGGGACACT GCTACCGGTA CTTTGCCCAT 
CGCCGGGCCT GGGAGGATGC AGAGAGAGAT TGCAGGCGCC GAGCTGGTCA CCTGACAAGC 
GTCCATTCGC CAGAAGAGCA CAAGTTCATT AACAGTTTTG GACATGAGAA TTCGTGGATC 
GGCCTGAATG ACCGGACAGT AGAGAGGGAC TTCCAGTGGA CAGACAACAC AGGACTGCAA 
TATGAGAACT GGAGGGAGAA GCAGCCGGAT AATTTCTTCG CAGGTGGGGA GGATTGTGTG 
GTGATGGTGG CACATGAGAG TGGGCGCTGG AATGATGTCC CCTGCAACTA CAACCTACCC 
TATGTCTGCA AGAAGGGTAC AGTGCTGTGT GGTCCCCCTC CAGCAGTGGA GAATGCCTCT 
CTTGTTGGTG TGCGCAAGAT CAAGTACAAT GTCCATGCCA CTGTGCGATA CCAGTGTGAT 
GAAGGATTCT CCCAGCACCG AGTGGCCACT ATCCGCTGCC GTAACAATGG GAAGTGGGAC 
CGGCCTCAGA TCATGTGCAT CAAACCCAGG CGGTCACATC GGATGCGTAG ACACCACCAC 
CATCCACACC GACATCACAA GCCTCGCAAG GAGCACAGAA AACACAAGAG ACACCCAGCG 
GAAGACTGGG AGAAAGACGA AGGGGATTTC TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncan ( NM_007789.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031815.2 Mus musculus neurocan (Ncan), mRNA.

Price & Availability↑

CloneID	OMu00807
Clone ID Related Accession (Same CDS sequence)	NM_007789.3
Accession Version	NM_007789.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	neurocan core protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX202471.1, BC065118.1 and BQ745524.1. On Nov 15, 2007 this sequence version replaced NM_007789.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065118.1, X84727.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGCCG GATCTGTGTG GGCCTCAGGC CTCCTGCTGC TGTGGCTGCT TCTCCTAGTG 
GCTGGGGATC AGGACACACA GGACACCACC GCCACGGAAA AGGGGCTTCG CATGCTGAAG 
TCAGGGTCAG GACCCGTCCG TGCTGCCCTG GCAGAGCTAG TGGCCCTGCC CTGCTTCTTT 
ACCCTGCAAC CACGGCTAAG CTCCCTGCGA GACATTCCTC GGATCAAGTG GACTAAGGTT 
CAGACTGCAT CAGGCCAGCG ACAGGATTTG CCAATCTTGG TGGCCAAAGA CAACGTGGTG 
CGTGTGGCCA AGGGCTGGCA GGGACGGGTG TCATTGCCTG CTTATCCCCG GCACAGAGCC 
AATGCTACCC TTCTGCTGGG GCCACTTCGA GCAAGCGACT CTGGGCTGTA TCGCTGCCAA 
GTGGTAAAGG GTATCGAAGA TGAGCAGGAC CTGGTAACCC TGGAAGTGAC AGGCGTCGTG 
TTCCATTATC GGGCGGCCCG GGACCGCTAT GCGCTGACCT TCGCTGAGGC CCAGGAGGCT 
TGTCGCCTAA GCTCTGCTAC CATCGCTGCC CCACGGCACC TGCAGGCTGC CTTTGAAGAT 
GGCTTTGACA ACTGCGACGC GGGCTGGCTC TCAGACCGCA CGGTTCGGTA CCCGATCACT 
CAGTCGCGTC CTGGTTGCTA TGGTGACCGC AGCAGTCTCC CGGGTGTTCG GAGCTACGGG 
AGACGCGACC CGCAGGAACT CTACGATGTC TACTGCTTTG CCCGCGAGCT AGGGGGTGAA 
GTCTTTTACG TGGGCCCGGC CCGCCGACTG ACCCTGGCGG GCGCGCGGGC ACAATGTCAG 
CGGCAGGGTG CAGCGCTGGC CTCGGTGGGG CAGTTGCACC TGGCCTGGCA CGAGGGCCTG 
GACCAGTGCG ACCCGGGCTG GCTGGCAGAC GGCAGCGTGC GCTACCCCAT CCAGACTCCG 
CGCCGGCGTT GCGGGGGCCC CGCCCCAGGT GTGCGCACTG TGTACCGCTT CGCCAACCGC 
ACCGGCTTTC CTGCGCCAGG AGCACGCTTC GACGCCTACT GCTTCCGAGC TCATCACCAT 
ACAGCACAAC ATGGAGATTC TGAGATCCCC TCATCTGGAG ATGAGGGGGA GATTGTGTCA 
GCAGAGGGGC CTCCAGGCCG AGAACTAAAG CCCAGCTTGG GGGAACAGGA GGTGATAGCA 
CCTGACTTCC AGGAACCTCT CATGTCCAGT GGAGAAGGCG AACCCCCAGA CTTGACTTGG 
ACACAAGCAC CTGAGGAGAC CCTTGGTTCT ACTCCAGGGG GTCCCACGCT GGCCTCATGG 
CCCTCTTCAG AAAAGTGGCT CTTTACGGGT GCCCCAAGCT CCATGGGTGT CTCCAGTCCC 
AGCGACATGG GAGTAGATAT GGAAGCGACA ACACCCTTGG GCACACAGGT AGCACCCACC 
CCCACGATGA GGAGGGGCCG CTTTAAAGGG TTGAATGGTC GACACTTCCA GCAGCAGGGC 
CCAGAAGACC AGCTGCCTGA GGTAGCAGAG CCCAGTGCCC AGCCTCCCAC CCTGGGAGCT 
ACTGCCAACC ACATGAGGCC TTCTGCAGCC ACAGAGGCTT CAGAGAGTGA CCAGAGCCAC 
AGTCCTTGGG CCATTCTGAC CAACGAAGTG GATGAGCCAG GAGCAGGCTC TCTTGGCAGC 
AGGAGTCTCC CAGAGTCCCT GATGTGGTCC CCGTCGTTGA TCTCACCCAG TGTCCCAAGC 
ACCGACAGTA CTCCTAGCGC GAAGCCAGGG GCAGCCGAGG CACCCAGTGT GAAGTCAGCC 
ATCCCCCACC TGCCCCGGCT GCCCTCAGAG CCCCCTGCTC CCTCTCCCGG GCCCTCAGAG 
GCCCTAAGTG CTGTCTCCCT CCAGGCATCC TCTGCTGATG GCTCCCCTGA CTTCCCCATT 
GTAGCCATGC TTCGAGCCCC CAAACTGTGG CTTCTGCCAC GCTCTACACT TGTCCCCAAT 
ATGACCCCAG TCCCACTGTC CCCAGCTTCT CCACTTCCCT CCTGGGTCCC AGAAGAACAG 
GCTGTCAGGC CTGTCAGCCT TGGAGCGGAA GACCTCGAAA CCCCATTTCA GACCACCATA 
GCTGCCCCAG TTGAAGCCAG CCACAGATCC CCTGATGCAG ATTCTATAGA AATCGAGGGG 
ACCAGCTCCA TGCGGGCTAC AAAGCACCCC ATCTCTGGCC CATGGGCTTC TTTGGACTCT 
AGTAATGTGA CGATGAATCC TGTCCCTTCT GATGCTGGCA TCCTAGGGAC TGAGTCTGGG 
GTCTTGGACT TACCAGGGAG TCCCACATCA GGCGGACAGG CCACTGTGGA GAAGGTGCTG 
GCCACCTGGC TACCACTGCC TGGCCAAGGA CTAGACCCTG GCTCCCAGTC CACACCCATG 
GAAGCCCATG GGGTTGCCGT GAGCATGGAA CCTACAGTGG CTTTGGAGGG AGGTGCCACC 
GAGGGCCCTA TGGAGGCCAC CAGGGAGGTG GTCCCCAGCA CTGCTGATGC CACTTGGGAG 
TCTGAATCCA GAAGTGCCAT TTCTAGCACC CACATAGCTG TGACCATGGC TAGGGCTCAG 
GGCATGCCCA CACTGACCTC TACAAGCTCC GAAGGCCACC CAGAGCCTAA GGGCCAGATG 
GTGGCCCAGG AGTCACTGGA GCCTCTCAAC ACTCTGCCTT CGCACCCCTG GTCATCCCTG 
GTGGTCCCCA TGGATGAAGT GGCCTCAGTT TCCTCAGGGG AACCCACAGG ACTGTGGGAC 
ATACCCAGCA CTCTGATACC TGTATCCTTG GGCTTGGATG AATCAGACCT GAATGTTGTG 
GCTGAGAGTC CAAGCGTGAA GGGCTTCTGG GAAGAGGTGG CCAGCGGGCA GGAAGATCCC 
ACAGATCCCT GCGAGAACAA CCCCTGCCTG CATGGGGGCA CCTGCCACAC CAACGGCACC 
GTGTATGGCT GTAGTTGTGA CCAGGGCTAT GCTGGGGAGA ATTGTGAAAT TGACATTGAT 
GACTGCTTAT GCAGCCCTTG CGAGAATGGG GGCACCTGCA TCGACGAGGT GAATGGTTTC 
ATCTGCCTCT GCCTCCCCAG CTATGGGGGC AGCCTGTGTG AGAAGGACAC AGAAGGCTGC 
GACCGTGGCT GGCACAAATT CCAGGGACAC TGCTACCGGT ACTTTGCCCA TCGCCGGGCC 
TGGGAGGATG CAGAGAGAGA TTGCAGGCGC CGAGCTGGTC ACCTGACAAG CGTCCATTCG 
CCAGAAGAGC ACAAGTTCAT TAACAGTTTT GGACATGAGA ATTCGTGGAT CGGCCTGAAT 
GACCGGACAG TAGAGAGGGA CTTCCAGTGG ACAGACAACA CAGGACTGCA ATATGAGAAC 
TGGAGGGAGA AGCAGCCGGA TAATTTCTTC GCAGGTGGGG AGGATTGTGT GGTGATGGTG 
GCACATGAGA GTGGGCGCTG GAATGATGTC CCCTGCAACT ACAACCTACC CTATGTCTGC 
AAGAAGGGTA CAGTGCTGTG TGGTCCCCCT CCAGCAGTGG AGAATGCCTC TCTTGTTGGT 
GTGCGCAAGA TCAAGTACAA TGTCCATGCC ACTGTGCGAT ACCAGTGTGA TGAAGGATTC 
TCCCAGCACC GAGTGGCCAC TATCCGCTGC CGTAACAATG GGAAGTGGGA CCGGCCTCAG 
ATCATGTGCA TCAAACCCAG GCGGTCACAT CGGATGCGTA GACACCACCA CCATCCACAC 
CGACATCACA AGCCTCGCAA GGAGCACAGA AAACACAAGA GACACCCAGC GGAAGACTGG 
GAGAAAGACG AAGGGGATTT CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031815.2
CDS	125..3931
Translation	MGAGSVWASGLLLLWLLLLVAGDQDTQDTTATEKGLRMLKSGSGPVRAALAELVALPCFFTLQPRLSSLRDIPRIKWTKVQTASGQRQDLPILVAKDNVVRVAKGWQGRVSLPAYPRHRANATLLLGPLRASDSGLYRCQVVKGIEDEQDLVTLEVTGVVFHYRAARDRYALTFAEAQEACRLSSATIAAPRHLQAAFEDGFDNCDAGWLSDRTVRYPITQSRPGCYGDRSSLPGVRSYGRRDPQELYDVYCFARELGGEVFYVGPARRLTLAGARAQCQRQGAALASVGQLHLAWHEGLDQCDPGWLADGSVRYPIQTPRRRCGGPAPGVRTVYRFANRTGFPAPGARFDAYCFRAHHHTAQHGDSEIPSSGDEGEIVSAEGPPGRELKPSLGEQEVIAPDFQEPLMSSGEGEPPDLTWTQAPEETLGSTPGGPTLASWPSSEKWLFTGAPSSMGVSSPSDMGVDMEATTPLGTQVAPTPTMRRGRFKGLNGRHFQQQGPEDQLPEVAEPSAQPPTLGATANHMRPSAATEASESDQSHSPWAILTNEVDEPGAGSLGSRSLPESLMWSPSLISPSVPSTDSTPSAKPGAAEAPSVKSAIPHLPRLPSEPPAPSPGPSEALSAVSLQASSADGSPDFPIVAMLRAPKLWLLPRSTLVPNMTPVPLSPASPLPSWVPEEQAVRPVSLGAEDLETPFQTTIAAPVEASHRSPDADSIEIEGTSSMRATKHPISGPWASLDSSNVTMNPVPSDAGILGTESGVLDLPGSPTSGGQATVEKVLATWLPLPGQGLDPGSQSTPMEAHGVAVSMEPTVALEGGATEGPMEATREVVPSTADATWESESRSAISSTHIAVTMARAQGMPTLTSTSSEGHPEPKGQMVAQESLEPLNTLPSHPWSSLVVPMDEVASVSSGEPTGLWDIPSTLIPVSLGLDESDLNVVAESPSVKGFWEEVASGQEDPTDPCENNPCLHGGTCHTNGTVYGCSCDQGYAGENCEIDIDDCLCSPCENGGTCIDEVNGFICLCLPSYGGSLCEKDTEGCDRGWHKFQGHCYRYFAHRRAWEDAERDCRRRAGHLTSVHSPEEHKFINSFGHENSWIGLNDRTVERDFQWTDNTGLQYENWREKQPDNFFAGGEDCVVMVAHESGRWNDVPCNYNLPYVCKKGTVLCGPPPAVENASLVGVRKIKYNVHATVRYQCDEGFSQHRVATIRCRNNGKWDRPQIMCIKPRRSHRMRRHHHHPHRHHKPRKEHRKHKRHPAEDWEKDEGDFC

Target ORF information:

RefSeq Version	NM_007789.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurocan (Ncan), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007789.3

ORF Insert Sequence:

ATGGGGGCCG GATCTGTGTG GGCCTCAGGC CTCCTGCTGC TGTGGCTGCT TCTCCTAGTG 
GCTGGGGATC AGGACACACA GGACACCACC GCCACGGAAA AGGGGCTTCG CATGCTGAAG 
TCAGGGTCAG GACCCGTCCG TGCTGCCCTG GCAGAGCTAG TGGCCCTGCC CTGCTTCTTT 
ACCCTGCAAC CACGGCTAAG CTCCCTGCGA GACATTCCTC GGATCAAGTG GACTAAGGTT 
CAGACTGCAT CAGGCCAGCG ACAGGATTTG CCAATCTTGG TGGCCAAAGA CAACGTGGTG 
CGTGTGGCCA AGGGCTGGCA GGGACGGGTG TCATTGCCTG CTTATCCCCG GCACAGAGCC 
AATGCTACCC TTCTGCTGGG GCCACTTCGA GCAAGCGACT CTGGGCTGTA TCGCTGCCAA 
GTGGTAAAGG GTATCGAAGA TGAGCAGGAC CTGGTAACCC TGGAAGTGAC AGGCGTCGTG 
TTCCATTATC GGGCGGCCCG GGACCGCTAT GCGCTGACCT TCGCTGAGGC CCAGGAGGCT 
TGTCGCCTAA GCTCTGCTAC CATCGCTGCC CCACGGCACC TGCAGGCTGC CTTTGAAGAT 
GGCTTTGACA ACTGCGACGC GGGCTGGCTC TCAGACCGCA CGGTTCGGTA CCCGATCACT 
CAGTCGCGTC CTGGTTGCTA TGGTGACCGC AGCAGTCTCC CGGGTGTTCG GAGCTACGGG 
AGACGCGACC CGCAGGAACT CTACGATGTC TACTGCTTTG CCCGCGAGCT AGGGGGTGAA 
GTCTTTTACG TGGGCCCGGC CCGCCGACTG ACCCTGGCGG GCGCGCGGGC ACAATGTCAG 
CGGCAGGGTG CAGCGCTGGC CTCGGTGGGG CAGTTGCACC TGGCCTGGCA CGAGGGCCTG 
GACCAGTGCG ACCCGGGCTG GCTGGCAGAC GGCAGCGTGC GCTACCCCAT CCAGACTCCG 
CGCCGGCGTT GCGGGGGCCC CGCCCCAGGT GTGCGCACTG TGTACCGCTT CGCCAACCGC 
ACCGGCTTTC CTGCGCCAGG AGCACGCTTC GACGCCTACT GCTTCCGAGC TCATCACCAT 
ACAGCACAAC ATGGAGATTC TGAGATCCCC TCATCTGGAG ATGAGGGGGA GATTGTGTCA 
GCAGAGGGGC CTCCAGGCCG AGAACTAAAG CCCAGCTTGG GGGAACAGGA GGTGATAGCA 
CCTGACTTCC AGGAACCTCT CATGTCCAGT GGAGAAGGCG AACCCCCAGA CTTGACTTGG 
ACACAAGCAC CTGAGGAGAC CCTTGGTTCT ACTCCAGGGG GTCCCACGCT GGCCTCATGG 
CCCTCTTCAG AAAAGTGGCT CTTTACGGGT GCCCCAAGCT CCATGGGTGT CTCCAGTCCC 
AGCGACATGG GAGTAGATAT GGAAGCGACA ACACCCTTGG GCACACAGGT AGCACCCACC 
CCCACGATGA GGAGGGGCCG CTTTAAAGGG TTGAATGGTC GACACTTCCA GCAGCAGGGC 
CCAGAAGACC AGCTGCCTGA GGTAGCAGAG CCCAGTGCCC AGCCTCCCAC CCTGGGAGCT 
ACTGCCAACC ACATGAGGCC TTCTGCAGCC ACAGAGGCTT CAGAGAGTGA CCAGAGCCAC 
AGTCCTTGGG CCATTCTGAC CAACGAAGTG GATGAGCCAG GAGCAGGCTC TCTTGGCAGC 
AGGAGTCTCC CAGAGTCCCT GATGTGGTCC CCGTCGTTGA TCTCACCCAG TGTCCCAAGC 
ACCGACAGTA CTCCTAGCGC GAAGCCAGGG GCAGCCGAGG CACCCAGTGT GAAGTCAGCC 
ATCCCCCACC TGCCCCGGCT GCCCTCAGAG CCCCCTGCTC CCTCTCCCGG GCCCTCAGAG 
GCCCTAAGTG CTGTCTCCCT CCAGGCATCC TCTGCTGATG GCTCCCCTGA CTTCCCCATT 
GTAGCCATGC TTCGAGCCCC CAAACTGTGG CTTCTGCCAC GCTCTACACT TGTCCCCAAT 
ATGACCCCAG TCCCACTGTC CCCAGCTTCT CCACTTCCCT CCTGGGTCCC AGAAGAACAG 
GCTGTCAGGC CTGTCAGCCT TGGAGCGGAA GACCTCGAAA CCCCATTTCA GACCACCATA 
GCTGCCCCAG TTGAAGCCAG CCACAGATCC CCTGATGCAG ATTCTATAGA AATCGAGGGG 
ACCAGCTCCA TGCGGGCTAC AAAGCACCCC ATCTCTGGCC CATGGGCTTC TTTGGACTCT 
AGTAATGTGA CGATGAATCC TGTCCCTTCT GATGCTGGCA TCCTAGGGAC TGAGTCTGGG 
GTCTTGGACT TACCAGGGAG TCCCACATCA GGCGGACAGG CCACTGTGGA GAAGGTGCTG 
GCCACCTGGC TACCACTGCC TGGCCAAGGA CTAGACCCTG GCTCCCAGTC CACACCCATG 
GAAGCCCATG GGGTTGCCGT GAGCATGGAA CCTACAGTGG CTTTGGAGGG AGGTGCCACC 
GAGGGCCCTA TGGAGGCCAC CAGGGAGGTG GTCCCCAGCA CTGCTGATGC CACTTGGGAG 
TCTGAATCCA GAAGTGCCAT TTCTAGCACC CACATAGCTG TGACCATGGC TAGGGCTCAG 
GGCATGCCCA CACTGACCTC TACAAGCTCC GAAGGCCACC CAGAGCCTAA GGGCCAGATG 
GTGGCCCAGG AGTCACTGGA GCCTCTCAAC ACTCTGCCTT CGCACCCCTG GTCATCCCTG 
GTGGTCCCCA TGGATGAAGT GGCCTCAGTT TCCTCAGGGG AACCCACAGG ACTGTGGGAC 
ATACCCAGCA CTCTGATACC TGTATCCTTG GGCTTGGATG AATCAGACCT GAATGTTGTG 
GCTGAGAGTC CAAGCGTGAA GGGCTTCTGG GAAGAGGTGG CCAGCGGGCA GGAAGATCCC 
ACAGATCCCT GCGAGAACAA CCCCTGCCTG CATGGGGGCA CCTGCCACAC CAACGGCACC 
GTGTATGGCT GTAGTTGTGA CCAGGGCTAT GCTGGGGAGA ATTGTGAAAT TGACATTGAT 
GACTGCTTAT GCAGCCCTTG CGAGAATGGG GGCACCTGCA TCGACGAGGT GAATGGTTTC 
ATCTGCCTCT GCCTCCCCAG CTATGGGGGC AGCCTGTGTG AGAAGGACAC AGAAGGCTGC 
GACCGTGGCT GGCACAAATT CCAGGGACAC TGCTACCGGT ACTTTGCCCA TCGCCGGGCC 
TGGGAGGATG CAGAGAGAGA TTGCAGGCGC CGAGCTGGTC ACCTGACAAG CGTCCATTCG 
CCAGAAGAGC ACAAGTTCAT TAACAGTTTT GGACATGAGA ATTCGTGGAT CGGCCTGAAT 
GACCGGACAG TAGAGAGGGA CTTCCAGTGG ACAGACAACA CAGGACTGCA ATATGAGAAC 
TGGAGGGAGA AGCAGCCGGA TAATTTCTTC GCAGGTGGGG AGGATTGTGT GGTGATGGTG 
GCACATGAGA GTGGGCGCTG GAATGATGTC CCCTGCAACT ACAACCTACC CTATGTCTGC 
AAGAAGGGTA CAGTGCTGTG TGGTCCCCCT CCAGCAGTGG AGAATGCCTC TCTTGTTGGT 
GTGCGCAAGA TCAAGTACAA TGTCCATGCC ACTGTGCGAT ACCAGTGTGA TGAAGGATTC 
TCCCAGCACC GAGTGGCCAC TATCCGCTGC CGTAACAATG GGAAGTGGGA CCGGCCTCAG 
ATCATGTGCA TCAAACCCAG GCGGTCACAT CGGATGCGTA GACACCACCA CCATCCACAC 
CGACATCACA AGCCTCGCAA GGAGCACAGA AAACACAAGA GACACCCAGC GGAAGACTGG 
GAGAAAGACG AAGGGGATTT CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncan ( XM_006509543.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509606.1 Mus musculus neurocan (Ncan), transcript variant X1, mRNA.

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CloneID	OMu40921
Clone ID Related Accession (Same CDS sequence)	XM_006509543.3 , XM_006509543.4
Accession Version	XM_006509543.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurocan core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006509543.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT CAGGGTCAGG ACCCGTCCGT GCTGCCCTGG CAGAGCTAGT GGCCCTGCCC 
TGCTTCTTTA CCCTGCAACC ACGGCTAAGC TCCCTGCGAG ACATTCCTCG GATCAAGTGG 
ACTAAGGTTC AGACTGCATC AGGCCAGCGA CAGGATTTGC CAATCTTGGT GGCCAAAGAC 
AACGTGGTGC GTGTGGCCAA GGGCTGGCAG GGACGGGTGT CATTGCCTGC TTATCCCCGG 
CACAGAGCCA ATGCTACCCT TCTGCTGGGG CCACTTCGAG CAAGCGACTC TGGGCTGTAT 
CGCTGCCAAG TGGTAAAGGG TATCGAAGAT GAGCAGGACC TGGTAACCCT GGAAGTGACA 
GGCGTCGTGT TCCATTATCG GGCGGCCCGG GACCGCTATG CGCTGACCTT CGCTGAGGCC 
CAGGAGGCTT GTCGCCTAAG CTCTGCTACC ATCGCTGCCC CACGGCACCT GCAGGCTGCC 
TTTGAAGATG GCTTTGACAA CTGCGACGCG GGCTGGCTCT CAGACCGCAC GGTTCGGTAC 
CCGATCACTC AGTCGCGTCC TGGTTGCTAT GGTGACCGCA GCAGTCTCCC GGGTGTTCGG 
AGCTACGGGA GACGCGACCC GCAGGAACTC TACGATGTCT ACTGCTTTGC CCGCGAGCTA 
GGGGGTGAAG TCTTTTACGT GGGCCCGGCC CGCCGACTGA CCCTGGCGGG CGCGCGGGCA 
CAATGTCAGC GGCAGGGTGC AGCGCTGGCC TCGGTGGGGC AGTTGCACCT GGCCTGGCAC 
GAGGGCCTGG ACCAGTGCGA CCCGGGCTGG CTGGCAGACG GCAGCGTGCG CTACCCCATC 
CAGACTCCGC GCCGGCGTTG CGGGGGCCCC GCCCCAGGTG TGCGCACTGT GTACCGCTTC 
GCCAACCGCA CCGGCTTTCC TGCGCCAGGA GCACGCTTCG ACGCCTACTG CTTCCGAGCT 
CATCACCATA CAGCACAACA TGGAGATTCT GAGATCCCCT CATCTGGAGA TGAGGGGGAG 
ATTGTGTCAG CAGAGGGGCC TCCAGGCCGA GAACTAAAGC CCAGCTTGGG GGAACAGGAG 
GTGATAGCAC CTGACTTCCA GGAACCTCTC ATGTCCAGTG GAGAAGGCGA ACCCCCAGAC 
TTGACTTGGA CACAAGCACC TGAGGAGACC CTTGGTTCTA CTCCAGGGGG TCCCACGCTG 
GCCTCATGGC CCTCTTCAGA AAAGTGGCTC TTTACGGGTG CCCCAAGCTC CATGGGTGTC 
TCCAGTCCCA GCGACATGGG AGTAGATATG GAAGCGACAA CACCCTTGGG CACACAGGTA 
GCACCCACCC CCACGATGAG GAGGGGCCGC TTTAAAGGGT TGAATGGTCG ACACTTCCAG 
CAGCAGGGCC CAGAAGACCA GCTGCCTGAG GTAGCAGAGC CCAGTGCCCA GCCTCCCACC 
CTGGGAGCTA CTGCCAACCA CATGAGGCCT TCTGCAGCCA CAGAGGCTTC AGAGAGTGAC 
CAGAGCCACA GTCCTTGGGC CATTCTGACC AACGAAGTGG ATGAGCCAGG AGCAGGCTCT 
CTTGGCAGCA GGAGTCTCCC AGAGTCCCTG ATGTGGTCCC CGTCGTTGAT CTCACCCAGT 
GTCCCAAGCA CCGACAGTAC TCCTAGCGCG AAGCCAGGGG CAGCCGAGGC ACCCAGTGTG 
AAGTCAGCCA TCCCCCACCT GCCCCGGCTG CCCTCAGAGC CCCCTGCTCC CTCTCCCGGG 
CCCTCAGAGG CCCTAAGTGC TGTCTCCCTC CAGGCATCCT CTGCTGATGG CTCCCCTGAC 
TTCCCCATTG TAGCCATGCT TCGAGCCCCC AAACTGTGGC TTCTGCCACG CTCTACACTT 
GTCCCCAATA TGACCCCAGT CCCACTGTCC CCAGCTTCTC CACTTCCCTC CTGGGTCCCA 
GAAGAACAGG CTGTCAGGCC TGTCAGCCTT GGAGCGGAAG ACCTCGAAAC CCCATTTCAG 
ACCACCATAG CTGCCCCAGT TGAAGCCAGC CACAGATCCC CTGATGCAGA TTCTATAGAA 
ATCGAGGGGA CCAGCTCCAT GCGGGCTACA AAGCACCCCA TCTCTGGCCC ATGGGCTTCT 
TTGGACTCTA GTAATGTGAC GATGAATCCT GTCCCTTCTG ATGCTGGCAT CCTAGGGACT 
GAGTCTGGGG TCTTGGACTT ACCAGGGAGT CCCACATCAG GCGGACAGGC CACTGTGGAG 
AAGGTGCTGG CCACCTGGCT ACCACTGCCT GGCCAAGGAC TAGACCCTGG CTCCCAGTCC 
ACACCCATGG AAGCCCATGG GGTTGCCGTG AGCATGGAAC CTACAGTGGC TTTGGAGGGA 
GGTGCCACCG AGGGCCCTAT GGAGGCCACC AGGGAGGTGG TCCCCAGCAC TGCTGATGCC 
ACTTGGGAGT CTGAATCCAG AAGTGCCATT TCTAGCACCC ACATAGCTGT GACCATGGCT 
AGGGCTCAGG GCATGCCCAC ACTGACCTCT ACAAGCTCCG AAGGCCACCC AGAGCCTAAG 
GGCCAGATGG TGGCCCAGGA GTCACTGGAG CCTCTCAACA CTCTGCCTTC GCACCCCTGG 
TCATCCCTGG TGGTCCCCAT GGATGAAGTG GCCTCAGTTT CCTCAGGGGA ACCCACAGGA 
CTGTGGGACA TACCCAGCAC TCTGATACCT GTATCCTTGG GCTTGGATGA ATCAGACCTG 
AATGTTGTGG CTGAGAGTCC AAGCGTGAAG GGCTTCTGGG AAGAGGTGGC CAGCGGGCAG 
GAAGATCCCA CAGATCCCTG CGAGAACAAC CCCTGCCTGC ATGGGGGCAC CTGCCACACC 
AACGGCACCG TGTATGGCTG TAGTTGTGAC CAGGGCTATG CTGGGGAGAA TTGTGAAATT 
GACATTGATG ACTGCTTATG CAGCCCTTGC GAGAATGGGG GCACCTGCAT CGACGAGGTG 
AATGGTTTCA TCTGCCTCTG CCTCCCCAGC TATGGGGGCA GCCTGTGTGA GAAGGACACA 
GAAGGCTGCG ACCGTGGCTG GCACAAATTC CAGGGACACT GCTACCGGTA CTTTGCCCAT 
CGCCGGGCCT GGGAGGATGC AGAGAGAGAT TGCAGGCGCC GAGCTGGTCA CCTGACAAGC 
GTCCATTCGC CAGAAGAGCA CAAGTTCATT AACAGTTTTG GACATGAGAA TTCGTGGATC 
GGCCTGAATG ACCGGACAGT AGAGAGGGAC TTCCAGTGGA CAGACAACAC AGGACTGCAA 
TATGAGAACT GGAGGGAGAA GCAGCCGGAT AATTTCTTCG CAGGTGGGGA GGATTGTGTG 
GTGATGGTGG CACATGAGAG TGGGCGCTGG AATGATGTCC CCTGCAACTA CAACCTACCC 
TATGTCTGCA AGAAGGGTAC AGTGCTGTGT GGTCCCCCTC CAGCAGTGGA GAATGCCTCT 
CTTGTTGGTG TGCGCAAGAT CAAGTACAAT GTCCATGCCA CTGTGCGATA CCAGTGTGAT 
GAAGGATTCT CCCAGCACCG AGTGGCCACT ATCCGCTGCC GTAACAATGG GAAGTGGGAC 
CGGCCTCAGA TCATGTGCAT CAAACCCAGG CGGTCACATC GGATGCGTAG ACACCACCAC 
CATCCACACC GACATCACAA GCCTCGCAAG GAGCACAGAA AACACAAGAG ACACCCAGCG 
GAAGACTGGG AGAAAGACGA AGGGGATTTC TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509606.1
CDS	175..3870
Translation	MLKSGSGPVRAALAELVALPCFFTLQPRLSSLRDIPRIKWTKVQTASGQRQDLPILVAKDNVVRVAKGWQGRVSLPAYPRHRANATLLLGPLRASDSGLYRCQVVKGIEDEQDLVTLEVTGVVFHYRAARDRYALTFAEAQEACRLSSATIAAPRHLQAAFEDGFDNCDAGWLSDRTVRYPITQSRPGCYGDRSSLPGVRSYGRRDPQELYDVYCFARELGGEVFYVGPARRLTLAGARAQCQRQGAALASVGQLHLAWHEGLDQCDPGWLADGSVRYPIQTPRRRCGGPAPGVRTVYRFANRTGFPAPGARFDAYCFRAHHHTAQHGDSEIPSSGDEGEIVSAEGPPGRELKPSLGEQEVIAPDFQEPLMSSGEGEPPDLTWTQAPEETLGSTPGGPTLASWPSSEKWLFTGAPSSMGVSSPSDMGVDMEATTPLGTQVAPTPTMRRGRFKGLNGRHFQQQGPEDQLPEVAEPSAQPPTLGATANHMRPSAATEASESDQSHSPWAILTNEVDEPGAGSLGSRSLPESLMWSPSLISPSVPSTDSTPSAKPGAAEAPSVKSAIPHLPRLPSEPPAPSPGPSEALSAVSLQASSADGSPDFPIVAMLRAPKLWLLPRSTLVPNMTPVPLSPASPLPSWVPEEQAVRPVSLGAEDLETPFQTTIAAPVEASHRSPDADSIEIEGTSSMRATKHPISGPWASLDSSNVTMNPVPSDAGILGTESGVLDLPGSPTSGGQATVEKVLATWLPLPGQGLDPGSQSTPMEAHGVAVSMEPTVALEGGATEGPMEATREVVPSTADATWESESRSAISSTHIAVTMARAQGMPTLTSTSSEGHPEPKGQMVAQESLEPLNTLPSHPWSSLVVPMDEVASVSSGEPTGLWDIPSTLIPVSLGLDESDLNVVAESPSVKGFWEEVASGQEDPTDPCENNPCLHGGTCHTNGTVYGCSCDQGYAGENCEIDIDDCLCSPCENGGTCIDEVNGFICLCLPSYGGSLCEKDTEGCDRGWHKFQGHCYRYFAHRRAWEDAERDCRRRAGHLTSVHSPEEHKFINSFGHENSWIGLNDRTVERDFQWTDNTGLQYENWREKQPDNFFAGGEDCVVMVAHESGRWNDVPCNYNLPYVCKKGTVLCGPPPAVENASLVGVRKIKYNVHATVRYQCDEGFSQHRVATIRCRNNGKWDRPQIMCIKPRRSHRMRRHHHHPHRHHKPRKEHRKHKRHPAEDWEKDEGDFC

Target ORF information:

RefSeq Version	XM_006509543.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurocan (Ncan), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509543.4

ORF Insert Sequence:

ATGCTGAAGT CAGGGTCAGG ACCCGTCCGT GCTGCCCTGG CAGAGCTAGT GGCCCTGCCC 
TGCTTCTTTA CCCTGCAACC ACGGCTAAGC TCCCTGCGAG ACATTCCTCG GATCAAGTGG 
ACTAAGGTTC AGACTGCATC AGGCCAGCGA CAGGATTTGC CAATCTTGGT GGCCAAAGAC 
AACGTGGTGC GTGTGGCCAA GGGCTGGCAG GGACGGGTGT CATTGCCTGC TTATCCCCGG 
CACAGAGCCA ATGCTACCCT TCTGCTGGGG CCACTTCGAG CAAGCGACTC TGGGCTGTAT 
CGCTGCCAAG TGGTAAAGGG TATCGAAGAT GAGCAGGACC TGGTAACCCT GGAAGTGACA 
GGCGTCGTGT TCCATTATCG GGCGGCCCGG GACCGCTATG CGCTGACCTT CGCTGAGGCC 
CAGGAGGCTT GTCGCCTAAG CTCTGCTACC ATCGCTGCCC CACGGCACCT GCAGGCTGCC 
TTTGAAGATG GCTTTGACAA CTGCGACGCG GGCTGGCTCT CAGACCGCAC GGTTCGGTAC 
CCGATCACTC AGTCGCGTCC TGGTTGCTAT GGTGACCGCA GCAGTCTCCC GGGTGTTCGG 
AGCTACGGGA GACGCGACCC GCAGGAACTC TACGATGTCT ACTGCTTTGC CCGCGAGCTA 
GGGGGTGAAG TCTTTTACGT GGGCCCGGCC CGCCGACTGA CCCTGGCGGG CGCGCGGGCA 
CAATGTCAGC GGCAGGGTGC AGCGCTGGCC TCGGTGGGGC AGTTGCACCT GGCCTGGCAC 
GAGGGCCTGG ACCAGTGCGA CCCGGGCTGG CTGGCAGACG GCAGCGTGCG CTACCCCATC 
CAGACTCCGC GCCGGCGTTG CGGGGGCCCC GCCCCAGGTG TGCGCACTGT GTACCGCTTC 
GCCAACCGCA CCGGCTTTCC TGCGCCAGGA GCACGCTTCG ACGCCTACTG CTTCCGAGCT 
CATCACCATA CAGCACAACA TGGAGATTCT GAGATCCCCT CATCTGGAGA TGAGGGGGAG 
ATTGTGTCAG CAGAGGGGCC TCCAGGCCGA GAACTAAAGC CCAGCTTGGG GGAACAGGAG 
GTGATAGCAC CTGACTTCCA GGAACCTCTC ATGTCCAGTG GAGAAGGCGA ACCCCCAGAC 
TTGACTTGGA CACAAGCACC TGAGGAGACC CTTGGTTCTA CTCCAGGGGG TCCCACGCTG 
GCCTCATGGC CCTCTTCAGA AAAGTGGCTC TTTACGGGTG CCCCAAGCTC CATGGGTGTC 
TCCAGTCCCA GCGACATGGG AGTAGATATG GAAGCGACAA CACCCTTGGG CACACAGGTA 
GCACCCACCC CCACGATGAG GAGGGGCCGC TTTAAAGGGT TGAATGGTCG ACACTTCCAG 
CAGCAGGGCC CAGAAGACCA GCTGCCTGAG GTAGCAGAGC CCAGTGCCCA GCCTCCCACC 
CTGGGAGCTA CTGCCAACCA CATGAGGCCT TCTGCAGCCA CAGAGGCTTC AGAGAGTGAC 
CAGAGCCACA GTCCTTGGGC CATTCTGACC AACGAAGTGG ATGAGCCAGG AGCAGGCTCT 
CTTGGCAGCA GGAGTCTCCC AGAGTCCCTG ATGTGGTCCC CGTCGTTGAT CTCACCCAGT 
GTCCCAAGCA CCGACAGTAC TCCTAGCGCG AAGCCAGGGG CAGCCGAGGC ACCCAGTGTG 
AAGTCAGCCA TCCCCCACCT GCCCCGGCTG CCCTCAGAGC CCCCTGCTCC CTCTCCCGGG 
CCCTCAGAGG CCCTAAGTGC TGTCTCCCTC CAGGCATCCT CTGCTGATGG CTCCCCTGAC 
TTCCCCATTG TAGCCATGCT TCGAGCCCCC AAACTGTGGC TTCTGCCACG CTCTACACTT 
GTCCCCAATA TGACCCCAGT CCCACTGTCC CCAGCTTCTC CACTTCCCTC CTGGGTCCCA 
GAAGAACAGG CTGTCAGGCC TGTCAGCCTT GGAGCGGAAG ACCTCGAAAC CCCATTTCAG 
ACCACCATAG CTGCCCCAGT TGAAGCCAGC CACAGATCCC CTGATGCAGA TTCTATAGAA 
ATCGAGGGGA CCAGCTCCAT GCGGGCTACA AAGCACCCCA TCTCTGGCCC ATGGGCTTCT 
TTGGACTCTA GTAATGTGAC GATGAATCCT GTCCCTTCTG ATGCTGGCAT CCTAGGGACT 
GAGTCTGGGG TCTTGGACTT ACCAGGGAGT CCCACATCAG GCGGACAGGC CACTGTGGAG 
AAGGTGCTGG CCACCTGGCT ACCACTGCCT GGCCAAGGAC TAGACCCTGG CTCCCAGTCC 
ACACCCATGG AAGCCCATGG GGTTGCCGTG AGCATGGAAC CTACAGTGGC TTTGGAGGGA 
GGTGCCACCG AGGGCCCTAT GGAGGCCACC AGGGAGGTGG TCCCCAGCAC TGCTGATGCC 
ACTTGGGAGT CTGAATCCAG AAGTGCCATT TCTAGCACCC ACATAGCTGT GACCATGGCT 
AGGGCTCAGG GCATGCCCAC ACTGACCTCT ACAAGCTCCG AAGGCCACCC AGAGCCTAAG 
GGCCAGATGG TGGCCCAGGA GTCACTGGAG CCTCTCAACA CTCTGCCTTC GCACCCCTGG 
TCATCCCTGG TGGTCCCCAT GGATGAAGTG GCCTCAGTTT CCTCAGGGGA ACCCACAGGA 
CTGTGGGACA TACCCAGCAC TCTGATACCT GTATCCTTGG GCTTGGATGA ATCAGACCTG 
AATGTTGTGG CTGAGAGTCC AAGCGTGAAG GGCTTCTGGG AAGAGGTGGC CAGCGGGCAG 
GAAGATCCCA CAGATCCCTG CGAGAACAAC CCCTGCCTGC ATGGGGGCAC CTGCCACACC 
AACGGCACCG TGTATGGCTG TAGTTGTGAC CAGGGCTATG CTGGGGAGAA TTGTGAAATT 
GACATTGATG ACTGCTTATG CAGCCCTTGC GAGAATGGGG GCACCTGCAT CGACGAGGTG 
AATGGTTTCA TCTGCCTCTG CCTCCCCAGC TATGGGGGCA GCCTGTGTGA GAAGGACACA 
GAAGGCTGCG ACCGTGGCTG GCACAAATTC CAGGGACACT GCTACCGGTA CTTTGCCCAT 
CGCCGGGCCT GGGAGGATGC AGAGAGAGAT TGCAGGCGCC GAGCTGGTCA CCTGACAAGC 
GTCCATTCGC CAGAAGAGCA CAAGTTCATT AACAGTTTTG GACATGAGAA TTCGTGGATC 
GGCCTGAATG ACCGGACAGT AGAGAGGGAC TTCCAGTGGA CAGACAACAC AGGACTGCAA 
TATGAGAACT GGAGGGAGAA GCAGCCGGAT AATTTCTTCG CAGGTGGGGA GGATTGTGTG 
GTGATGGTGG CACATGAGAG TGGGCGCTGG AATGATGTCC CCTGCAACTA CAACCTACCC 
TATGTCTGCA AGAAGGGTAC AGTGCTGTGT GGTCCCCCTC CAGCAGTGGA GAATGCCTCT 
CTTGTTGGTG TGCGCAAGAT CAAGTACAAT GTCCATGCCA CTGTGCGATA CCAGTGTGAT 
GAAGGATTCT CCCAGCACCG AGTGGCCACT ATCCGCTGCC GTAACAATGG GAAGTGGGAC 
CGGCCTCAGA TCATGTGCAT CAAACCCAGG CGGTCACATC GGATGCGTAG ACACCACCAC 
CATCCACACC GACATCACAA GCCTCGCAAG GAGCACAGAA AACACAAGAG ACACCCAGCG 
GAAGACTGGG AGAAAGACGA AGGGGATTTC TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Intrinsic cellular and molecular properties of in vivo hippocampal synaptic plasticity are altered in the absence of key synaptic matrix molecules.
Hippocampus27(8)920-933(2017 Aug)
Jansen S,Gottschling C,Faissner A,Manahan-Vaughan D

?-amyloid increases neurocan expression through regulating Sox9 in astrocytes: A potential relationship between Sox9 and chondroitin sulfate proteoglycans in Alzheimer's disease.
Brain research1646377-383(2016 09)
Yan H,Zhu X,Xie J,Zhao Y,Liu X

Muscleblind-like 1 is required for normal heart valve development in vivo.
BMC developmental biology1536(2015 Oct)
Coram RJ,Stillwagon SJ,Guggilam A,Jenkins MW,Swanson MS,Ladd AN

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Ncan cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

ORF Insert Sequence:

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