Lamb1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Lamb1
Entrez Gene ID	16777
Full Name	laminin B1
Synonyms	C77966,C80098,C81607,D130003D08Rik,Lamb-1,Lamb1-1
General protein information	Preferred Names laminin B1 Names laminin subunit beta-1 laminin B1 chain laminin B1 subunit 1 laminin-1 subunit beta laminin-10 subunit beta laminin-111 laminin-12 subunit beta laminin-2 subunit beta laminin-6 subunit beta laminin-8 subunit beta
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12 A2\|12 13.39 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006514993.1	XP_006515056.1	laminin subunit beta-1 isoform X1
NM_008482.3	NP_032508.3	laminin subunit beta-1 precursor
NM_008482.2	NP_032508.2	laminin subunit beta-1
XM_006514992.3	XP_006515055.2	laminin subunit beta-1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LAMB1	NP_001193448.1
Homo sapiens (human)	LAMB1	NP_002282.2
Canis lupus familiaris (dog)	LAMB1	XP_533089.4
Xenopus tropicalis (tropical clawed frog)	lamb1	XP_002933140.2
Pan troglodytes (chimpanzee)	LAMB1	XP_001165667.2
Mus musculus (house mouse)	Lamb1	NP_032508.2
Gallus gallus (chicken)	LAMB1	XP_415943.3
	LAMB1	XP_001090393.2
Rattus norvegicus (Norway rat)	Lamb1	XP_003750185.1
Danio rerio (zebrafish)	lamb1a	NP_775382.1

Gene Ontology
Bibliography

Related articles in PubMed

Casoni F, Croci L, Bosone C, D'Ambrosio R, Badaloni A, Gaudesi D, Barili V, Sarna JR, Tessarollo L, Cremona O, Hawkes R, Warming S, Consalez GG
Development (Cambridge, England)144(20)3686-3697(2017 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that laminin alpha2beta1gamma1 (Lm211) can inhibit neuregulin 1 type III (Nrg1III) by limiting protein kinase A (PKA) activation, which is required to initiate myelination.
Title: Laminin 211 inhibits protein kinase A in Schwann cells to modulate neuregulin 1 type III-driven myelination.

A new mutant mouse (lamb1t) exhibits intermittent dystonic hindlimb movements and postures when awake, and hyperextension when asleep.
Title: A dystonia-like movement disorder with brain and spinal neuronal defects is caused by mutation of the mouse laminin ?1 subunit, Lamb1.

This study reveals a previously unidentified role of laminin-111 in early stem cell differentiation.
Title: Biologically-active laminin-111 fragment that modulates the epithelial-to-mesenchymal transition in embryonic stem cells.

These results suggest the possibility that up-regulation of LAMB1 in podocytes, should it become achievable, would likely lessen the severity of nephrotic syndrome in patients carrying LAMB2 mutations.
Title: Forced expression of laminin beta1 in podocytes prevents nephrotic syndrome in mice lacking laminin beta2, a model for Pierson syndrome.

Laminin beta(1) and gamma(1) N-terminal domains share the galactose-binding domain-like fold.
Title: Computational modeling of laminin N-terminal domains using sparse distance constraints from disulfide bonds and chemical cross-linking.

The following Lamb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lamb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21998	NM_008482.2 Latest version!	Mus musculus laminin B1 (Lamb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1350.30 $1929.00
OMu47246	NM_008482.3 Latest version!	Mus musculus laminin B1 (Lamb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu47246	XM_006514992.3 Latest version!	Mus musculus laminin B1 (Lamb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu47246	XM_006514993.1 Latest version!	Mus musculus laminin B1 (Lamb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lamb1 ( NM_008482.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032508.2 Mus musculus laminin B1 (Lamb1), mRNA.

Price & Availability↑

CloneID	OMu21998
Clone ID Related Accession (Same CDS sequence)	NM_008482.2
Accession Version	NM_008482.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Mus musculus(house mouse)
Product	laminin subunit beta-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ093872.1 and AK147313.1. On Sep 14, 2006 this sequence version replaced NM_008482.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147313.1, M15525.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGGGC CCCTCTCCTC TCTCCCAACA TTTGCCTTTT CTCCCCGCTA CCTCTCCAGA 
AAGGAAGACC CGAAGAAAAG ACAGGCAGCT TGCCTGCTGC GTCCTCCTTC CCGTGCCGCG 
TCCCCTCGTC TGCGAGGACT GGACATGGGG CTGCTCCAGG TGTTCGCCTT TGGTGTCCTA 
GCCCTATGGG GCACCCGAGT GTGCGCTCAG GAACCGGAGT TCAGCTATGG CTGCGCAGAA 
GGCAGCTGCT ACCCTGCCAC TGGCGACCTT CTCATCGGCC GAGCGCAAAA GCTCTCCGTG 
ACTTCGACAT GTGGACTGCA CAAACCAGAG CCCTACTGTA TTGTTAGCCA CCTGCAGGAG 
GACAAGAAAT GCTTCATATG TGACTCCCGA GACCCTTATC ACGAGACCCT CAACCCCGAC 
AGCCATCTCA TTGAGAACGT GGTCACCACA TTTGCTCCAA ACCGCCTTAA GATCTGGTGG 
CAATCGGAAA ATGGTGTGGA GAACGTGACC ATCCAACTGG ACCTGGAAGC AGAATTCCAT 
TTCACTCATC TCATCATGAC CTTCAAGACA TTCCGCCCAG CCGCCATGCT GATCGAGCGG 
TCTTCTGACT TTGGGAAGGC TTGGGGCGTG TACAGATACT TCGCCTACGA CTGTGAGAGC 
TCGTTCCCAG GCATTTCAAC TGGACCCATG AAGAAAGTGG ATGACATCAT CTGTGACTCT 
CGATATTCTG ACATTGAGCC CTCGACAGAA GGAGAGGTAA TATTTCGTGC TTTAGATCCT 
GCTTTCAAAA TTGAAGACCC TTATAGTCCA AGGATACAGA ATCTATTAAA AATCACCAAC 
TTGAGAATCA AGTTTGTGAA ACTGCACACC TTGGGGGATA ACCTTTTGGA CTCCAGAATG 
GAAATCCGAG AGAAGTACTA TTACGCTGTT TATGATATGG TGGTTCGAGG GAACTGCTTC 
TGCTATGGCC ACGCCAGTGA ATGCGCCCCT GTGGATGGAG TCAATGAAGA AGTGGAAGGA 
ATGGTTCACG GGCACTGCAT GTGCAGACAC AACACCAAAG GCCTGAACTG TGAGCTGTGC 
ATGGATTTCT ACCACGATTT GCCGTGGAGA CCTGCTGAAG GCCGGAACAG CAACGCCTGC 
AAAAAATGTA ACTGCAATGA ACATTCCAGC TCGTGTCACT TTGACATGGC AGTCTTCCTG 
GCTACTGGCA ACGTCAGCGG GGGAGTGTGT GATAACTGTC AGCACAACAC CATGGGGCGC 
AACTGTGAAC AGTGCAAACC GTTCTACTTC CAGCACCCTG AGAGGGACAT CCGGGACCCC 
AATCTCTGTG AACCATGTAC CTGTGACCCA GCTGGTTCTG AGAATGGCGG GATCTGTGAT 
GGGTACACTG ATTTTTCTGT GGGTCTCATT GCTGGTCAGT GTCGGTGCAA ATTGCACGTG 
GAGGGAGAGC GCTGTGATGT TTGTAAAGAA GGCTTCTACG ACTTAAGTGC TGAAGACCCG 
TATGGTTGTA AATCATGTGC TTGCAATCCT CTGGGAACAA TTCCTGGTGG GAATCCTTGT 
GATTCTGAGA CTGGCTACTG CTACTGTAAG CGCCTGGTGA CAGGACAGCG CTGTGACCAG 
TGCCTGCCGC AGCACTGGGG TTTAAGCAAT GATTTGGATG GGTGTCGACC TTGTGACTGT 
GACCTTGGAG GGGCGCTGAA CAATAGCTGC TCCGAGGACT CCGGCCAGTG CTCCTGCCTG 
CCCCACATGA TTGGGCGGCA GTGTAACGAG GTGGAGTCCG GTTACTACTT CACCACCCTG 
GACCACTACA TCTACGAAGC CGAGGAAGCC AATCTGGGGC CTGGAGTCAT TGTGGTGGAA 
AGGCAGTACA TTCAGGACCG CATTCCTTCC TGGACAGGAC CTGGGTTCGT CCGGGTGCCT 
GAAGGGGCTT ATTTGGAGTT TTTCATTGAC AACATACCGT ATTCCATGGA GTATGAAATC 
CTGATTCGCT ATGAGCCACA GCTGCCGGAC CACTGGGAGA AAGCTGTCAT CACTGTACAG 
CGGCCGGGGA AGATTCCAGC CAGCAGCCGA TGTGGTAACA CCGTTCCCGA TGATGACAAC 
CAGGTGGTGT CCTTGTCACC GGGCTCAAGA TACGTTGTCC TCCCTCGCCC CGTGTGCTTT 
GAGAAGGGAA TGAACTACAC GGTGAGGTTG GAGCTGCCCC AGTATACGGC ATCGGGCAGT 
GACGTGGAGA GCCCTTACAC GTTCATCGAC TCGCTTGTTC TCATGCCCTA CTGTAAATCG 
CTGGACATCT TCACTGTTGG CGGCTCAGGC GATGGGGAGG TCACCAATAG TGCCTGGGAA 
ACCTTCCAGC GCTACAGGTG TCTGGAGAAC AGCAGGAGTG TGGTAAAAAC ACCCATGACA 
GATGTCTGCA GAAACATTAT CTTCAGCATT TCTGCCTTGA TTCACCAGAC GGGCCTTGCT 
TGTGAATGTG ACCCCCAGGG ATCTCTGAGT TCTGTGTGTG ACCCCAATGG TGGCCAGTGC 
CAGTGCCGTC CTAATGTGGT TGGAAGAACC TGCAACAGGT GTGCCCCGGG CACCTTTGGC 
TTTGGCCCCA ACGGATGCAA ACCTTGTGAC TGCCATCTGC AAGGGTCTGC CAGTGCCTTC 
TGCGATGCGA TCACTGGCCA GTGCCACTGT TTCCAGGGCA TCTATGCTCG GCAGTGTGAC 
CGATGTCTCC CTGGGTATTG GGGCTTTCCC AGCTGCCAGC CCTGCCAGTG TAATGGTCAT 
GCTCTAGACT GTGACACAGT GACAGGGGAG TGTCTGAGCT GTCAGGACTA CACCACGGGC 
CACAACTGCG AAAGGTGCCT GGCTGGCTAC TACGGTGATC CCATCATTGG GTCAGGAGAC 
CACTGTCGCC CTTGCCCTTG TCCTGATGGT CCTGACAGTG GACGACAGTT TGCCAGGAGC 
TGTTATCAAG ACCCCGTCAC TCTCCAGCTT GCGTGTGTTT GTGATCCTGG GTACATTGGC 
TCCAGATGTG ATGACTGTGC CTCTGGATTT TTTGGCAATC CCTCAGACTT TGGGGGTTCA 
TGTCAACCAT GTCAGTGCCA CCACAACATT GACACTACCG ATCCAGAAGC CTGTGACAAG 
GAGACGGGAC GATGCCTCAA GTGCCTGTAC CACACAGAAG GGGACCATTG CCAGCTCTGC 
CAGTATGGGT ACTACGGCGA TGCTCTTCGG CAAGACTGTA GAAAGTGTGT CTGCAATTAC 
CTGGGCACGG TGAAGGAACA TTGTAATGGC TCTGACTGCC ACTGTGACAA AGCCACTGGT 
CAGTGCTCGT GCCTTCCCAA TGTGATCGGG CAGAACTGTG ACCGGTGTGC GCCCAACACC 
TGGCAGCTGG CTAGCGGGAC TGGCTGCGGG CCCTGCAATT GCAATGCTGC GCATTCCTTT 
GGGCCATCCT GCAACGAGTT CACAGGGCAG TGCCAGTGCA TGCCGGGCTT TGGAGGCCGA 
ACCTGCAGCG AGTGCCAGGA GCTCTTCTGG GGAGACCCTG ATGTGGAATG CCGAGCCTGT 
GACTGTGATC CCAGGGGCAT TGAGACACCT CAGTGTGACC AGTCCACGGG CCAGTGTGTC 
TGTGTGGAGG GTGTAGAGGG TCCTCGCTGC GACAAGTGCA CCAGAGGTTA CTCGGGGGTC 
TTTCCTGACT GCACACCCTG CCACCAGTGC TTTGCTCTCT GGGATGCTAT CATTGGTGAG 
CTGACCAACA GGACCCACAA ATTCCTGGAG AAAGCCAAGG CTCTGAAAAT CAGTGGTGTG 
ATTGGTCCCT ACCGAGAGAC CGTGGACTCT GTAGAGAAGA AAGTCAATGA GATAAAAGAC 
ATCCTGGCCC AGAGCCCAGC AGCGGAACCA CTGAAAAACA TTGGCATTCT CTTCGAGGAG 
GCAGAGAAAC TAACCAAAGA TGTCACAGAA AAGATGGCGC AGGTAGAAGT GAAATTAACT 
GATACAGCTT CACAGAGTAA CAGCACAGCT GGAGAGCTCG GCGCACTGCA GGCAGAAGCA 
GAGAGCCTTG ACAAGACCGT GAAGGAGCTG GCAGAACAGC TGGAGTTTAT CAAAAACTCC 
GATATTCAGG GCGCCTTGGA TAGCATCACC AAGTATTTCC AGATGTCTCT TGAGGCAGAG 
AAGCGGGTGA ATGCCTCCAC CACAGACCCC AACAGCACTG TGGAGCAGTC TGCCCTCACG 
CGAGACAGAG TAGAAGATCT GATGTTGGAG CGAGAGTCTC CGTTCAAGGA GCAGCAGGAG 
GAACAGGCAC GCCTCCTGGA CGAACTGGCC GGCAAACTGC AAAGTCTCGA CCTGTCGGCT 
GTTGCACAGA TGACCTGTGG AACACCTCCA GGGGCTGACT GTTCTGAAAG TGAATGTGGT 
GGCCCCAACT GCAGAACTGA CGAAGGAGAG AAGAAGTGTG GGGGGCCTGG CTGTGGTGGT 
CTGGTCACTG TGGCCCACAG TGCTTGGCAG AAAGCCATGG ATTTTGACCG TGATGTCCTG 
AGTGCCCTGG CTGAAGTCGA ACAGCTCTCC AAGATGGTCT CTGAAGCAAA AGTGAGAGCA 
GATGAGGCGA AGCAGAATGC GCAGGATGTC CTGTTAAAAA CAAATGCTAC CAAAGAAAAA 
GTGGACAAGA GCAACGAGGA CCTGCGGAAC CTCATCAAGC AGATCAGAAA CTTCCTGACT 
GAGGATAGTG CTGATCTAGA CAGTATTGAA GCAGTTGCTA ATGAAGTACT GAAAATGGAA 
ATGCCTAGCA CGCCACAGCA GTTACAGAAC CTAACAGAAG ACATTCGGGA GCGAGTTGAA 
ACCCTCTCTC AAGTAGAGGT TATTTTGCAG CAGAGTGCAG CTGACATTGC CAGAGCTGAG 
CTGTTGCTTG AGGAAGCTAA GAGAGCAAGC AAAAGTGCAA CAGATGTTAA AGTCACTGCA 
GACATGGTGA AGGAAGCATT AGAAGAAGCA GAAAAGGCCC AGGTTGCAGC AGAGAAGGCG 
ATTAAACAAG CTGATGAGGA TATCCAAGGA ACCCAAAACC TGCTAACATC GATTGAATCT 
GAAACGGCAG CTTCTGAGGA AACCCTGACC AACGCCTCCC AGCGCATCAG CAAGCTTGAG 
AGGAACGTGG AAGAGCTTAA GCGTAAAGCT GCCCAGAACT CTGGGGAGGC AGAATATATC 
GAAAAAGTAG TATATTCTGT AAAACAGAAT GCAGACGATG TTAAGAAGAC TCTAGATGGC 
GAACTTGATG AAAAGTATAA GAAGGTAGAA AGTTTAATTG CCCAAAAAAC TGAAGAGTCA 
GCAGATGCCA GGAGGAAAGC TGAGCTGCTA CAAAATGAAG CAAAAACACT CTTGGCTCAA 
GCTAACAGCA AGCTCCAGCT GTTGGAAGAC TTAGAAAGAA AATATGAGGA CAATCAAAAA 
TACTTAGAAG ATAAAGCTCA AGAATTGGTG CGACTGGAAG GAGAGGTTCG CTCCCTCCTT 
AAGGACATAA GTGAGAAAGT TGCGGTTTAC AGCACCTGCT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032508.2
CDS	128..5632
Translation	MEGPLSSLPTFAFSPRYLSRKEDPKKRQAACLLRPPSRAASPRLRGLDMGLLQVFAFGVLALWGTRVCAQEPEFSYGCAEGSCYPATGDLLIGRAQKLSVTSTCGLHKPEPYCIVSHLQEDKKCFICDSRDPYHETLNPDSHLIENVVTTFAPNRLKIWWQSENGVENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKAWGVYRYFAYDCESSFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIFRALDPAFKIEDPYSPRIQNLLKITNLRIKFVKLHTLGDNLLDSRMEIREKYYYAVYDMVVRGNCFCYGHASECAPVDGVNEEVEGMVHGHCMCRHNTKGLNCELCMDFYHDLPWRPAEGRNSNACKKCNCNEHSSSCHFDMAVFLATGNVSGGVCDNCQHNTMGRNCEQCKPFYFQHPERDIRDPNLCEPCTCDPAGSENGGICDGYTDFSVGLIAGQCRCKLHVEGERCDVCKEGFYDLSAEDPYGCKSCACNPLGTIPGGNPCDSETGYCYCKRLVTGQRCDQCLPQHWGLSNDLDGCRPCDCDLGGALNNSCSEDSGQCSCLPHMIGRQCNEVESGYYFTTLDHYIYEAEEANLGPGVIVVERQYIQDRIPSWTGPGFVRVPEGAYLEFFIDNIPYSMEYEILIRYEPQLPDHWEKAVITVQRPGKIPASSRCGNTVPDDDNQVVSLSPGSRYVVLPRPVCFEKGMNYTVRLELPQYTASGSDVESPYTFIDSLVLMPYCKSLDIFTVGGSGDGEVTNSAWETFQRYRCLENSRSVVKTPMTDVCRNIIFSISALIHQTGLACECDPQGSLSSVCDPNGGQCQCRPNVVGRTCNRCAPGTFGFGPNGCKPCDCHLQGSASAFCDAITGQCHCFQGIYARQCDRCLPGYWGFPSCQPCQCNGHALDCDTVTGECLSCQDYTTGHNCERCLAGYYGDPIIGSGDHCRPCPCPDGPDSGRQFARSCYQDPVTLQLACVCDPGYIGSRCDDCASGFFGNPSDFGGSCQPCQCHHNIDTTDPEACDKETGRCLKCLYHTEGDHCQLCQYGYYGDALRQDCRKCVCNYLGTVKEHCNGSDCHCDKATGQCSCLPNVIGQNCDRCAPNTWQLASGTGCGPCNCNAAHSFGPSCNEFTGQCQCMPGFGGRTCSECQELFWGDPDVECRACDCDPRGIETPQCDQSTGQCVCVEGVEGPRCDKCTRGYSGVFPDCTPCHQCFALWDAIIGELTNRTHKFLEKAKALKISGVIGPYRETVDSVEKKVNEIKDILAQSPAAEPLKNIGILFEEAEKLTKDVTEKMAQVEVKLTDTASQSNSTAGELGALQAEAESLDKTVKELAEQLEFIKNSDIQGALDSITKYFQMSLEAEKRVNASTTDPNSTVEQSALTRDRVEDLMLERESPFKEQQEEQARLLDELAGKLQSLDLSAVAQMTCGTPPGADCSESECGGPNCRTDEGEKKCGGPGCGGLVTVAHSAWQKAMDFDRDVLSALAEVEQLSKMVSEAKVRADEAKQNAQDVLLKTNATKEKVDKSNEDLRNLIKQIRNFLTEDSADLDSIEAVANEVLKMEMPSTPQQLQNLTEDIRERVETLSQVEVILQQSAADIARAELLLEEAKRASKSATDVKVTADMVKEALEEAEKAQVAAEKAIKQADEDIQGTQNLLTSIESETAASEETLTNASQRISKLERNVEELKRKAAQNSGEAEYIEKVVYSVKQNADDVKKTLDGELDEKYKKVESLIAQKTEESADARRKAELLQNEAKTLLAQANSKLQLLEDLERKYEDNQKYLEDKAQELVRLEGEVRSLLKDISEKVAVYSTCL

Target ORF information:

RefSeq Version	NM_008482.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus laminin B1 (Lamb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008482.2

ORF Insert Sequence:

ATGGAAGGGC CCCTCTCCTC TCTCCCAACA TTTGCCTTTT CTCCCCGCTA CCTCTCCAGA 
AAGGAAGACC CGAAGAAAAG ACAGGCAGCT TGCCTGCTGC GTCCTCCTTC CCGTGCCGCG 
TCCCCTCGTC TGCGAGGACT GGACATGGGG CTGCTCCAGG TGTTCGCCTT TGGTGTCCTA 
GCCCTATGGG GCACCCGAGT GTGCGCTCAG GAACCGGAGT TCAGCTATGG CTGCGCAGAA 
GGCAGCTGCT ACCCTGCCAC TGGCGACCTT CTCATCGGCC GAGCGCAAAA GCTCTCCGTG 
ACTTCGACAT GTGGACTGCA CAAACCAGAG CCCTACTGTA TTGTTAGCCA CCTGCAGGAG 
GACAAGAAAT GCTTCATATG TGACTCCCGA GACCCTTATC ACGAGACCCT CAACCCCGAC 
AGCCATCTCA TTGAGAACGT GGTCACCACA TTTGCTCCAA ACCGCCTTAA GATCTGGTGG 
CAATCGGAAA ATGGTGTGGA GAACGTGACC ATCCAACTGG ACCTGGAAGC AGAATTCCAT 
TTCACTCATC TCATCATGAC CTTCAAGACA TTCCGCCCAG CCGCCATGCT GATCGAGCGG 
TCTTCTGACT TTGGGAAGGC TTGGGGCGTG TACAGATACT TCGCCTACGA CTGTGAGAGC 
TCGTTCCCAG GCATTTCAAC TGGACCCATG AAGAAAGTGG ATGACATCAT CTGTGACTCT 
CGATATTCTG ACATTGAGCC CTCGACAGAA GGAGAGGTAA TATTTCGTGC TTTAGATCCT 
GCTTTCAAAA TTGAAGACCC TTATAGTCCA AGGATACAGA ATCTATTAAA AATCACCAAC 
TTGAGAATCA AGTTTGTGAA ACTGCACACC TTGGGGGATA ACCTTTTGGA CTCCAGAATG 
GAAATCCGAG AGAAGTACTA TTACGCTGTT TATGATATGG TGGTTCGAGG GAACTGCTTC 
TGCTATGGCC ACGCCAGTGA ATGCGCCCCT GTGGATGGAG TCAATGAAGA AGTGGAAGGA 
ATGGTTCACG GGCACTGCAT GTGCAGACAC AACACCAAAG GCCTGAACTG TGAGCTGTGC 
ATGGATTTCT ACCACGATTT GCCGTGGAGA CCTGCTGAAG GCCGGAACAG CAACGCCTGC 
AAAAAATGTA ACTGCAATGA ACATTCCAGC TCGTGTCACT TTGACATGGC AGTCTTCCTG 
GCTACTGGCA ACGTCAGCGG GGGAGTGTGT GATAACTGTC AGCACAACAC CATGGGGCGC 
AACTGTGAAC AGTGCAAACC GTTCTACTTC CAGCACCCTG AGAGGGACAT CCGGGACCCC 
AATCTCTGTG AACCATGTAC CTGTGACCCA GCTGGTTCTG AGAATGGCGG GATCTGTGAT 
GGGTACACTG ATTTTTCTGT GGGTCTCATT GCTGGTCAGT GTCGGTGCAA ATTGCACGTG 
GAGGGAGAGC GCTGTGATGT TTGTAAAGAA GGCTTCTACG ACTTAAGTGC TGAAGACCCG 
TATGGTTGTA AATCATGTGC TTGCAATCCT CTGGGAACAA TTCCTGGTGG GAATCCTTGT 
GATTCTGAGA CTGGCTACTG CTACTGTAAG CGCCTGGTGA CAGGACAGCG CTGTGACCAG 
TGCCTGCCGC AGCACTGGGG TTTAAGCAAT GATTTGGATG GGTGTCGACC TTGTGACTGT 
GACCTTGGAG GGGCGCTGAA CAATAGCTGC TCCGAGGACT CCGGCCAGTG CTCCTGCCTG 
CCCCACATGA TTGGGCGGCA GTGTAACGAG GTGGAGTCCG GTTACTACTT CACCACCCTG 
GACCACTACA TCTACGAAGC CGAGGAAGCC AATCTGGGGC CTGGAGTCAT TGTGGTGGAA 
AGGCAGTACA TTCAGGACCG CATTCCTTCC TGGACAGGAC CTGGGTTCGT CCGGGTGCCT 
GAAGGGGCTT ATTTGGAGTT TTTCATTGAC AACATACCGT ATTCCATGGA GTATGAAATC 
CTGATTCGCT ATGAGCCACA GCTGCCGGAC CACTGGGAGA AAGCTGTCAT CACTGTACAG 
CGGCCGGGGA AGATTCCAGC CAGCAGCCGA TGTGGTAACA CCGTTCCCGA TGATGACAAC 
CAGGTGGTGT CCTTGTCACC GGGCTCAAGA TACGTTGTCC TCCCTCGCCC CGTGTGCTTT 
GAGAAGGGAA TGAACTACAC GGTGAGGTTG GAGCTGCCCC AGTATACGGC ATCGGGCAGT 
GACGTGGAGA GCCCTTACAC GTTCATCGAC TCGCTTGTTC TCATGCCCTA CTGTAAATCG 
CTGGACATCT TCACTGTTGG CGGCTCAGGC GATGGGGAGG TCACCAATAG TGCCTGGGAA 
ACCTTCCAGC GCTACAGGTG TCTGGAGAAC AGCAGGAGTG TGGTAAAAAC ACCCATGACA 
GATGTCTGCA GAAACATTAT CTTCAGCATT TCTGCCTTGA TTCACCAGAC GGGCCTTGCT 
TGTGAATGTG ACCCCCAGGG ATCTCTGAGT TCTGTGTGTG ACCCCAATGG TGGCCAGTGC 
CAGTGCCGTC CTAATGTGGT TGGAAGAACC TGCAACAGGT GTGCCCCGGG CACCTTTGGC 
TTTGGCCCCA ACGGATGCAA ACCTTGTGAC TGCCATCTGC AAGGGTCTGC CAGTGCCTTC 
TGCGATGCGA TCACTGGCCA GTGCCACTGT TTCCAGGGCA TCTATGCTCG GCAGTGTGAC 
CGATGTCTCC CTGGGTATTG GGGCTTTCCC AGCTGCCAGC CCTGCCAGTG TAATGGTCAT 
GCTCTAGACT GTGACACAGT GACAGGGGAG TGTCTGAGCT GTCAGGACTA CACCACGGGC 
CACAACTGCG AAAGGTGCCT GGCTGGCTAC TACGGTGATC CCATCATTGG GTCAGGAGAC 
CACTGTCGCC CTTGCCCTTG TCCTGATGGT CCTGACAGTG GACGACAGTT TGCCAGGAGC 
TGTTATCAAG ACCCCGTCAC TCTCCAGCTT GCGTGTGTTT GTGATCCTGG GTACATTGGC 
TCCAGATGTG ATGACTGTGC CTCTGGATTT TTTGGCAATC CCTCAGACTT TGGGGGTTCA 
TGTCAACCAT GTCAGTGCCA CCACAACATT GACACTACCG ATCCAGAAGC CTGTGACAAG 
GAGACGGGAC GATGCCTCAA GTGCCTGTAC CACACAGAAG GGGACCATTG CCAGCTCTGC 
CAGTATGGGT ACTACGGCGA TGCTCTTCGG CAAGACTGTA GAAAGTGTGT CTGCAATTAC 
CTGGGCACGG TGAAGGAACA TTGTAATGGC TCTGACTGCC ACTGTGACAA AGCCACTGGT 
CAGTGCTCGT GCCTTCCCAA TGTGATCGGG CAGAACTGTG ACCGGTGTGC GCCCAACACC 
TGGCAGCTGG CTAGCGGGAC TGGCTGCGGG CCCTGCAATT GCAATGCTGC GCATTCCTTT 
GGGCCATCCT GCAACGAGTT CACAGGGCAG TGCCAGTGCA TGCCGGGCTT TGGAGGCCGA 
ACCTGCAGCG AGTGCCAGGA GCTCTTCTGG GGAGACCCTG ATGTGGAATG CCGAGCCTGT 
GACTGTGATC CCAGGGGCAT TGAGACACCT CAGTGTGACC AGTCCACGGG CCAGTGTGTC 
TGTGTGGAGG GTGTAGAGGG TCCTCGCTGC GACAAGTGCA CCAGAGGTTA CTCGGGGGTC 
TTTCCTGACT GCACACCCTG CCACCAGTGC TTTGCTCTCT GGGATGCTAT CATTGGTGAG 
CTGACCAACA GGACCCACAA ATTCCTGGAG AAAGCCAAGG CTCTGAAAAT CAGTGGTGTG 
ATTGGTCCCT ACCGAGAGAC CGTGGACTCT GTAGAGAAGA AAGTCAATGA GATAAAAGAC 
ATCCTGGCCC AGAGCCCAGC AGCGGAACCA CTGAAAAACA TTGGCATTCT CTTCGAGGAG 
GCAGAGAAAC TAACCAAAGA TGTCACAGAA AAGATGGCGC AGGTAGAAGT GAAATTAACT 
GATACAGCTT CACAGAGTAA CAGCACAGCT GGAGAGCTCG GCGCACTGCA GGCAGAAGCA 
GAGAGCCTTG ACAAGACCGT GAAGGAGCTG GCAGAACAGC TGGAGTTTAT CAAAAACTCC 
GATATTCAGG GCGCCTTGGA TAGCATCACC AAGTATTTCC AGATGTCTCT TGAGGCAGAG 
AAGCGGGTGA ATGCCTCCAC CACAGACCCC AACAGCACTG TGGAGCAGTC TGCCCTCACG 
CGAGACAGAG TAGAAGATCT GATGTTGGAG CGAGAGTCTC CGTTCAAGGA GCAGCAGGAG 
GAACAGGCAC GCCTCCTGGA CGAACTGGCC GGCAAACTGC AAAGTCTCGA CCTGTCGGCT 
GTTGCACAGA TGACCTGTGG AACACCTCCA GGGGCTGACT GTTCTGAAAG TGAATGTGGT 
GGCCCCAACT GCAGAACTGA CGAAGGAGAG AAGAAGTGTG GGGGGCCTGG CTGTGGTGGT 
CTGGTCACTG TGGCCCACAG TGCTTGGCAG AAAGCCATGG ATTTTGACCG TGATGTCCTG 
AGTGCCCTGG CTGAAGTCGA ACAGCTCTCC AAGATGGTCT CTGAAGCAAA AGTGAGAGCA 
GATGAGGCGA AGCAGAATGC GCAGGATGTC CTGTTAAAAA CAAATGCTAC CAAAGAAAAA 
GTGGACAAGA GCAACGAGGA CCTGCGGAAC CTCATCAAGC AGATCAGAAA CTTCCTGACT 
GAGGATAGTG CTGATCTAGA CAGTATTGAA GCAGTTGCTA ATGAAGTACT GAAAATGGAA 
ATGCCTAGCA CGCCACAGCA GTTACAGAAC CTAACAGAAG ACATTCGGGA GCGAGTTGAA 
ACCCTCTCTC AAGTAGAGGT TATTTTGCAG CAGAGTGCAG CTGACATTGC CAGAGCTGAG 
CTGTTGCTTG AGGAAGCTAA GAGAGCAAGC AAAAGTGCAA CAGATGTTAA AGTCACTGCA 
GACATGGTGA AGGAAGCATT AGAAGAAGCA GAAAAGGCCC AGGTTGCAGC AGAGAAGGCG 
ATTAAACAAG CTGATGAGGA TATCCAAGGA ACCCAAAACC TGCTAACATC GATTGAATCT 
GAAACGGCAG CTTCTGAGGA AACCCTGACC AACGCCTCCC AGCGCATCAG CAAGCTTGAG 
AGGAACGTGG AAGAGCTTAA GCGTAAAGCT GCCCAGAACT CTGGGGAGGC AGAATATATC 
GAAAAAGTAG TATATTCTGT AAAACAGAAT GCAGACGATG TTAAGAAGAC TCTAGATGGC 
GAACTTGATG AAAAGTATAA GAAGGTAGAA AGTTTAATTG CCCAAAAAAC TGAAGAGTCA 
GCAGATGCCA GGAGGAAAGC TGAGCTGCTA CAAAATGAAG CAAAAACACT CTTGGCTCAA 
GCTAACAGCA AGCTCCAGCT GTTGGAAGAC TTAGAAAGAA AATATGAGGA CAATCAAAAA 
TACTTAGAAG ATAAAGCTCA AGAATTGGTG CGACTGGAAG GAGAGGTTCG CTCCCTCCTT 
AAGGACATAA GTGAGAAAGT TGCGGTTTAC AGCACCTGCT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lamb1 ( NM_008482.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032508.3 Mus musculus laminin B1 (Lamb1), mRNA.

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CloneID	OMu47246
Clone ID Related Accession (Same CDS sequence)	XM_006514993.1 , NM_008482.3 , XM_006514992.3
Accession Version	NM_008482.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	laminin subunit beta-1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT571245.12 and CR974423.21. On May 24, 2019 this sequence version replaced NM_008482.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M15525.1, SRR5314784.95746.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGCTGC TCCAGGTGTT CGCCTTTGGT GTCCTAGCCC TATGGGGCAC CCGAGTGTGC 
GCTCAGGAAC CGGAGTTCAG CTATGGCTGC GCAGAAGGCA GCTGCTACCC TGCCACTGGC 
GACCTTCTCA TCGGCCGAGC GCAAAAGCTC TCCGTGACTT CGACATGTGG ACTGCACAAA 
CCAGAGCCCT ACTGTATTGT TAGCCACCTG CAGGAGGACA AGAAATGCTT CATATGTGAC 
TCCCGAGACC CTTATCACGA GACCCTCAAC CCCGACAGCC ATCTCATTGA GAACGTGGTC 
ACCACATTTG CTCCAAACCG CCTTAAGATC TGGTGGCAAT CGGAAAATGG TGTGGAGAAC 
GTGACCATCC AACTGGACCT GGAAGCAGAA TTCCATTTCA CTCATCTCAT CATGACCTTC 
AAGACATTCC GCCCAGCCGC CATGCTGATC GAGCGGTCTT CTGACTTTGG GAAGGCTTGG 
GGCGTGTACA GATACTTCGC CTACGACTGT GAGAGCTCGT TCCCAGGCAT TTCAACTGGA 
CCCATGAAGA AAGTGGATGA CATCATCTGT GACTCTCGAT ATTCTGACAT TGAGCCCTCG 
ACAGAAGGAG AGGTAATATT TCGTGCTTTA GATCCTGCTT TCAAAATTGA AGACCCTTAT 
AGTCCAAGGA TACAGAATCT ATTAAAAATC ACCAACTTGA GAATCAAGTT TGTGAAACTG 
CACACCTTGG GGGATAACCT TTTGGACTCC AGAATGGAAA TCCGAGAGAA GTACTATTAC 
GCTGTTTATG ATATGGTGGT TCGAGGGAAC TGCTTCTGCT ATGGCCACGC CAGTGAATGC 
GCCCCTGTGG ATGGAGTCAA TGAAGAAGTG GAAGGAATGG TTCACGGGCA CTGCATGTGC 
AGACACAACA CCAAAGGCCT GAACTGTGAG CTGTGCATGG ATTTCTACCA CGATTTGCCG 
TGGAGACCTG CTGAAGGCCG GAACAGCAAC GCCTGCAAAA AATGTAACTG CAATGAACAT 
TCCAGCTCGT GTCACTTTGA CATGGCAGTC TTCCTGGCTA CTGGCAACGT CAGCGGGGGA 
GTGTGTGATA ACTGTCAGCA CAACACCATG GGGCGCAACT GTGAACAGTG CAAACCGTTC 
TACTTCCAGC ACCCTGAGAG GGACATCCGG GACCCCAATC TCTGTGAACC ATGTACCTGT 
GACCCAGCTG GTTCTGAGAA TGGCGGGATC TGTGATGGGT ACACTGATTT TTCTGTGGGT 
CTCATTGCTG GTCAGTGTCG GTGCAAATTG CACGTGGAGG GAGAGCGCTG TGATGTTTGT 
AAAGAAGGCT TCTACGACTT AAGTGCTGAA GACCCGTATG GTTGTAAATC ATGTGCTTGC 
AATCCTCTGG GAACAATTCC TGGTGGGAAT CCTTGTGATT CTGAGACTGG CTACTGCTAC 
TGTAAGCGCC TGGTGACAGG ACAGCGCTGT GACCAGTGCC TGCCGCAGCA CTGGGGTTTA 
AGCAATGATT TGGATGGGTG TCGACCTTGT GACTGTGACC TTGGAGGGGC GCTGAACAAT 
AGCTGCTCCG AGGACTCCGG CCAGTGCTCC TGCCTGCCCC ACATGATTGG GCGGCAGTGT 
AACGAGGTGG AGTCCGGTTA CTACTTCACC ACCCTGGACC ACTACATCTA CGAAGCCGAG 
GAAGCCAATC TGGGGCCTGG AGTCATTGTG GTGGAAAGGC AGTACATTCA GGACCGCATT 
CCTTCCTGGA CAGGACCTGG GTTCGTCCGG GTGCCTGAAG GGGCTTATTT GGAGTTTTTC 
ATTGACAACA TACCGTATTC CATGGAGTAT GAAATCCTGA TTCGCTATGA GCCACAGCTG 
CCGGACCACT GGGAGAAAGC TGTCATCACT GTACAGCGGC CGGGGAAGAT TCCAGCCAGC 
AGCCGATGTG GTAACACCGT TCCCGATGAT GACAACCAGG TGGTGTCCTT GTCACCGGGC 
TCAAGATACG TTGTCCTCCC TCGCCCCGTG TGCTTTGAGA AGGGAATGAA CTACACGGTG 
AGGTTGGAGC TGCCCCAGTA TACGGCATCG GGCAGTGACG TGGAGAGCCC TTACACGTTC 
ATCGACTCGC TTGTTCTCAT GCCCTACTGT AAATCGCTGG ACATCTTCAC TGTTGGCGGC 
TCAGGCGATG GGGAGGTCAC CAATAGTGCC TGGGAAACCT TCCAGCGCTA CAGGTGTCTG 
GAGAACAGCA GGAGTGTGGT AAAAACACCC ATGACAGATG TCTGCAGAAA CATTATCTTC 
AGCATTTCTG CCTTGATTCA CCAGACGGGC CTTGCTTGTG AATGTGACCC CCAGGGATCT 
CTGAGTTCTG TGTGTGACCC CAATGGTGGC CAGTGCCAGT GCCGTCCTAA TGTGGTTGGA 
AGAACCTGCA ACAGGTGTGC CCCGGGCACC TTTGGCTTTG GCCCCAACGG ATGCAAACCT 
TGTGACTGCC ATCTGCAAGG GTCTGCCAGT GCCTTCTGCG ATGCGATCAC TGGCCAGTGC 
CACTGTTTCC AGGGCATCTA TGCTCGGCAG TGTGACCGAT GTCTCCCTGG GTATTGGGGC 
TTTCCCAGCT GCCAGCCCTG CCAGTGTAAT GGTCATGCTC TAGACTGTGA CACAGTGACA 
GGGGAGTGTC TGAGCTGTCA GGACTACACC ACGGGCCACA ACTGCGAAAG GTGCCTGGCT 
GGCTACTACG GTGATCCCAT CATTGGGTCA GGAGACCACT GTCGCCCTTG CCCTTGTCCT 
GATGGTCCTG ACAGTGGACG ACAGTTTGCC AGGAGCTGTT ATCAAGACCC CGTCACTCTC 
CAGCTTGCGT GTGTTTGTGA TCCTGGGTAC ATTGGCTCCA GATGTGATGA CTGTGCCTCT 
GGATTTTTTG GCAATCCCTC AGACTTTGGG GGTTCATGTC AACCATGTCA GTGCCACCAC 
AACATTGACA CTACCGATCC AGAAGCCTGT GACAAGGAGA CGGGACGATG CCTCAAGTGC 
CTGTACCACA CAGAAGGGGA CCATTGCCAG CTCTGCCAGT ATGGGTACTA CGGCGATGCT 
CTTCGGCAAG ACTGTAGAAA GTGTGTCTGC AATTACCTGG GCACGGTGAA GGAACATTGT 
AATGGCTCTG ACTGCCACTG TGACAAAGCC ACTGGTCAGT GCTCGTGCCT TCCCAATGTG 
ATCGGGCAGA ACTGTGACCG GTGTGCGCCC AACACCTGGC AGCTGGCTAG CGGGACTGGC 
TGCGGGCCCT GCAATTGCAA TGCTGCGCAT TCCTTTGGGC CATCCTGCAA CGAGTTCACA 
GGGCAGTGCC AGTGCATGCC GGGCTTTGGA GGCCGAACCT GCAGCGAGTG CCAGGAGCTC 
TTCTGGGGAG ACCCTGATGT GGAATGCCGA GCCTGTGACT GTGATCCCAG GGGCATTGAG 
ACACCTCAGT GTGACCAGTC CACGGGCCAG TGTGTCTGTG TGGAGGGTGT AGAGGGTCCT 
CGCTGCGACA AGTGCACCAG AGGTTACTCG GGGGTCTTTC CTGACTGCAC ACCCTGCCAC 
CAGTGCTTTG CTCTCTGGGA TGCTATCATT GGTGAGCTGA CCAACAGGAC CCACAAATTC 
CTGGAGAAAG CCAAGGCTCT GAAAATCAGT GGTGTGATTG GTCCCTACCG AGAGACCGTG 
GACTCTGTAG AGAAGAAAGT CAATGAGATA AAAGACATCC TGGCCCAGAG CCCAGCAGCG 
GAACCACTGA AAAACATTGG CATTCTCTTC GAGGAGGCAG AGAAACTAAC CAAAGATGTC 
ACAGAAAAGA TGGCGCAGGT AGAAGTGAAA TTAACTGATA CAGCTTCACA GAGTAACAGC 
ACAGCTGGAG AGCTCGGCGC ACTGCAGGCA GAAGCAGAGA GCCTTGACAA GACCGTGAAG 
GAGCTGGCAG AACAGCTGGA GTTTATCAAA AACTCCGATA TTCAGGGCGC CTTGGATAGC 
ATCACCAAGT ATTTCCAGAT GTCTCTTGAG GCAGAGAAGC GGGTGAATGC CTCCACCACA 
GACCCCAACA GCACTGTGGA GCAGTCTGCC CTCACGCGAG ACAGAGTAGA AGATCTGATG 
TTGGAGCGAG AGTCTCCGTT CAAGGAGCAG CAGGAGGAAC AGGCACGCCT CCTGGACGAA 
CTGGCCGGCA AACTGCAAAG TCTCGACCTG TCGGCTGTTG CACAGATGAC CTGTGGAACA 
CCTCCAGGGG CTGACTGTTC TGAAAGTGAA TGTGGTGGCC CCAACTGCAG AACTGACGAA 
GGAGAGAAGA AGTGTGGGGG GCCTGGCTGT GGTGGTCTGG TCACTGTGGC CCACAGTGCT 
TGGCAGAAAG CCATGGATTT TGACCGTGAT GTCCTGAGTG CCCTGGCTGA AGTCGAACAG 
CTCTCCAAGA TGGTCTCTGA AGCAAAAGTG AGAGCAGATG AGGCGAAGCA GAATGCGCAG 
GATGTCCTGT TAAAAACAAA TGCTACCAAA GAAAAAGTGG ACAAGAGCAA CGAGGACCTG 
CGGAACCTCA TCAAGCAGAT CAGAAACTTC CTGACTGAGG ATAGTGCTGA TCTAGACAGT 
ATTGAAGCAG TTGCTAATGA AGTACTGAAA ATGGAAATGC CTAGCACGCC ACAGCAGTTA 
CAGAACCTAA CAGAAGACAT TCGGGAGCGA GTTGAAACCC TCTCTCAAGT AGAGGTTATT 
TTGCAGCAGA GTGCAGCTGA CATTGCCAGA GCTGAGCTGT TGCTTGAGGA AGCTAAGAGA 
GCAAGCAAAA GTGCAACAGA TGTTAAAGTC ACTGCAGACA TGGTGAAGGA AGCATTAGAA 
GAAGCAGAAA AGGCCCAGGT TGCAGCAGAG AAGGCGATTA AACAAGCTGA TGAGGATATC 
CAAGGAACCC AAAACCTGCT AACATCGATT GAATCTGAAA CGGCAGCTTC TGAGGAAACC 
CTGACCAACG CCTCCCAGCG CATCAGCAAG CTTGAGAGGA ACGTGGAAGA GCTTAAGCGT 
AAAGCTGCCC AGAACTCTGG GGAGGCAGAA TATATCGAAA AAGTAGTATA TTCTGTAAAA 
CAGAATGCAG ACGATGTTAA GAAGACTCTA GATGGCGAAC TTGATGAAAA GTATAAGAAG 
GTAGAAAGTT TAATTGCCCA AAAAACTGAA GAGTCAGCAG ATGCCAGGAG GAAAGCTGAG 
CTGCTACAAA ATGAAGCAAA AACACTCTTG GCTCAAGCTA ACAGCAAGCT CCAGCTGTTG 
GAAGACTTAG AAAGAAAATA TGAGGACAAT CAAAAATACT TAGAAGATAA AGCTCAAGAA 
TTGGTGCGAC TGGAAGGAGA GGTTCGCTCC CTCCTTAAGG ACATAAGTGA GAAAGTTGCG 
GTTTACAGCA CCTGCTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032508.3
CDS	273..5633
Translation	MGLLQVFAFGVLALWGTRVCAQEPEFSYGCAEGSCYPATGDLLIGRAQKLSVTSTCGLHKPEPYCIVSHLQEDKKCFICDSRDPYHETLNPDSHLIENVVTTFAPNRLKIWWQSENGVENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKAWGVYRYFAYDCESSFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIFRALDPAFKIEDPYSPRIQNLLKITNLRIKFVKLHTLGDNLLDSRMEIREKYYYAVYDMVVRGNCFCYGHASECAPVDGVNEEVEGMVHGHCMCRHNTKGLNCELCMDFYHDLPWRPAEGRNSNACKKCNCNEHSSSCHFDMAVFLATGNVSGGVCDNCQHNTMGRNCEQCKPFYFQHPERDIRDPNLCEPCTCDPAGSENGGICDGYTDFSVGLIAGQCRCKLHVEGERCDVCKEGFYDLSAEDPYGCKSCACNPLGTIPGGNPCDSETGYCYCKRLVTGQRCDQCLPQHWGLSNDLDGCRPCDCDLGGALNNSCSEDSGQCSCLPHMIGRQCNEVESGYYFTTLDHYIYEAEEANLGPGVIVVERQYIQDRIPSWTGPGFVRVPEGAYLEFFIDNIPYSMEYEILIRYEPQLPDHWEKAVITVQRPGKIPASSRCGNTVPDDDNQVVSLSPGSRYVVLPRPVCFEKGMNYTVRLELPQYTASGSDVESPYTFIDSLVLMPYCKSLDIFTVGGSGDGEVTNSAWETFQRYRCLENSRSVVKTPMTDVCRNIIFSISALIHQTGLACECDPQGSLSSVCDPNGGQCQCRPNVVGRTCNRCAPGTFGFGPNGCKPCDCHLQGSASAFCDAITGQCHCFQGIYARQCDRCLPGYWGFPSCQPCQCNGHALDCDTVTGECLSCQDYTTGHNCERCLAGYYGDPIIGSGDHCRPCPCPDGPDSGRQFARSCYQDPVTLQLACVCDPGYIGSRCDDCASGFFGNPSDFGGSCQPCQCHHNIDTTDPEACDKETGRCLKCLYHTEGDHCQLCQYGYYGDALRQDCRKCVCNYLGTVKEHCNGSDCHCDKATGQCSCLPNVIGQNCDRCAPNTWQLASGTGCGPCNCNAAHSFGPSCNEFTGQCQCMPGFGGRTCSECQELFWGDPDVECRACDCDPRGIETPQCDQSTGQCVCVEGVEGPRCDKCTRGYSGVFPDCTPCHQCFALWDAIIGELTNRTHKFLEKAKALKISGVIGPYRETVDSVEKKVNEIKDILAQSPAAEPLKNIGILFEEAEKLTKDVTEKMAQVEVKLTDTASQSNSTAGELGALQAEAESLDKTVKELAEQLEFIKNSDIQGALDSITKYFQMSLEAEKRVNASTTDPNSTVEQSALTRDRVEDLMLERESPFKEQQEEQARLLDELAGKLQSLDLSAVAQMTCGTPPGADCSESECGGPNCRTDEGEKKCGGPGCGGLVTVAHSAWQKAMDFDRDVLSALAEVEQLSKMVSEAKVRADEAKQNAQDVLLKTNATKEKVDKSNEDLRNLIKQIRNFLTEDSADLDSIEAVANEVLKMEMPSTPQQLQNLTEDIRERVETLSQVEVILQQSAADIARAELLLEEAKRASKSATDVKVTADMVKEALEEAEKAQVAAEKAIKQADEDIQGTQNLLTSIESETAASEETLTNASQRISKLERNVEELKRKAAQNSGEAEYIEKVVYSVKQNADDVKKTLDGELDEKYKKVESLIAQKTEESADARRKAELLQNEAKTLLAQANSKLQLLEDLERKYEDNQKYLEDKAQELVRLEGEVRSLLKDISEKVAVYSTCL

Target ORF information:

RefSeq Version	NM_008482.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus laminin B1 (Lamb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008482.3

ORF Insert Sequence:

ATGGGGCTGC TCCAGGTGTT CGCCTTTGGT GTCCTAGCCC TATGGGGCAC CCGAGTGTGC 
GCTCAGGAAC CGGAGTTCAG CTATGGCTGC GCAGAAGGCA GCTGCTACCC TGCCACTGGC 
GACCTTCTCA TCGGCCGAGC GCAAAAGCTC TCCGTGACTT CGACATGTGG ACTGCACAAA 
CCAGAGCCCT ACTGTATTGT TAGCCACCTG CAGGAGGACA AGAAATGCTT CATATGTGAC 
TCCCGAGACC CTTATCACGA GACCCTCAAC CCCGACAGCC ATCTCATTGA GAACGTGGTC 
ACCACATTTG CTCCAAACCG CCTTAAGATC TGGTGGCAAT CGGAAAATGG TGTGGAGAAC 
GTGACCATCC AACTGGACCT GGAAGCAGAA TTCCATTTCA CTCATCTCAT CATGACCTTC 
AAGACATTCC GCCCAGCCGC CATGCTGATC GAGCGGTCTT CTGACTTTGG GAAGGCTTGG 
GGCGTGTACA GATACTTCGC CTACGACTGT GAGAGCTCGT TCCCAGGCAT TTCAACTGGA 
CCCATGAAGA AAGTGGATGA CATCATCTGT GACTCTCGAT ATTCTGACAT TGAGCCCTCG 
ACAGAAGGAG AGGTAATATT TCGTGCTTTA GATCCTGCTT TCAAAATTGA AGACCCTTAT 
AGTCCAAGGA TACAGAATCT ATTAAAAATC ACCAACTTGA GAATCAAGTT TGTGAAACTG 
CACACCTTGG GGGATAACCT TTTGGACTCC AGAATGGAAA TCCGAGAGAA GTACTATTAC 
GCTGTTTATG ATATGGTGGT TCGAGGGAAC TGCTTCTGCT ATGGCCACGC CAGTGAATGC 
GCCCCTGTGG ATGGAGTCAA TGAAGAAGTG GAAGGAATGG TTCACGGGCA CTGCATGTGC 
AGACACAACA CCAAAGGCCT GAACTGTGAG CTGTGCATGG ATTTCTACCA CGATTTGCCG 
TGGAGACCTG CTGAAGGCCG GAACAGCAAC GCCTGCAAAA AATGTAACTG CAATGAACAT 
TCCAGCTCGT GTCACTTTGA CATGGCAGTC TTCCTGGCTA CTGGCAACGT CAGCGGGGGA 
GTGTGTGATA ACTGTCAGCA CAACACCATG GGGCGCAACT GTGAACAGTG CAAACCGTTC 
TACTTCCAGC ACCCTGAGAG GGACATCCGG GACCCCAATC TCTGTGAACC ATGTACCTGT 
GACCCAGCTG GTTCTGAGAA TGGCGGGATC TGTGATGGGT ACACTGATTT TTCTGTGGGT 
CTCATTGCTG GTCAGTGTCG GTGCAAATTG CACGTGGAGG GAGAGCGCTG TGATGTTTGT 
AAAGAAGGCT TCTACGACTT AAGTGCTGAA GACCCGTATG GTTGTAAATC ATGTGCTTGC 
AATCCTCTGG GAACAATTCC TGGTGGGAAT CCTTGTGATT CTGAGACTGG CTACTGCTAC 
TGTAAGCGCC TGGTGACAGG ACAGCGCTGT GACCAGTGCC TGCCGCAGCA CTGGGGTTTA 
AGCAATGATT TGGATGGGTG TCGACCTTGT GACTGTGACC TTGGAGGGGC GCTGAACAAT 
AGCTGCTCCG AGGACTCCGG CCAGTGCTCC TGCCTGCCCC ACATGATTGG GCGGCAGTGT 
AACGAGGTGG AGTCCGGTTA CTACTTCACC ACCCTGGACC ACTACATCTA CGAAGCCGAG 
GAAGCCAATC TGGGGCCTGG AGTCATTGTG GTGGAAAGGC AGTACATTCA GGACCGCATT 
CCTTCCTGGA CAGGACCTGG GTTCGTCCGG GTGCCTGAAG GGGCTTATTT GGAGTTTTTC 
ATTGACAACA TACCGTATTC CATGGAGTAT GAAATCCTGA TTCGCTATGA GCCACAGCTG 
CCGGACCACT GGGAGAAAGC TGTCATCACT GTACAGCGGC CGGGGAAGAT TCCAGCCAGC 
AGCCGATGTG GTAACACCGT TCCCGATGAT GACAACCAGG TGGTGTCCTT GTCACCGGGC 
TCAAGATACG TTGTCCTCCC TCGCCCCGTG TGCTTTGAGA AGGGAATGAA CTACACGGTG 
AGGTTGGAGC TGCCCCAGTA TACGGCATCG GGCAGTGACG TGGAGAGCCC TTACACGTTC 
ATCGACTCGC TTGTTCTCAT GCCCTACTGT AAATCGCTGG ACATCTTCAC TGTTGGCGGC 
TCAGGCGATG GGGAGGTCAC CAATAGTGCC TGGGAAACCT TCCAGCGCTA CAGGTGTCTG 
GAGAACAGCA GGAGTGTGGT AAAAACACCC ATGACAGATG TCTGCAGAAA CATTATCTTC 
AGCATTTCTG CCTTGATTCA CCAGACGGGC CTTGCTTGTG AATGTGACCC CCAGGGATCT 
CTGAGTTCTG TGTGTGACCC CAATGGTGGC CAGTGCCAGT GCCGTCCTAA TGTGGTTGGA 
AGAACCTGCA ACAGGTGTGC CCCGGGCACC TTTGGCTTTG GCCCCAACGG ATGCAAACCT 
TGTGACTGCC ATCTGCAAGG GTCTGCCAGT GCCTTCTGCG ATGCGATCAC TGGCCAGTGC 
CACTGTTTCC AGGGCATCTA TGCTCGGCAG TGTGACCGAT GTCTCCCTGG GTATTGGGGC 
TTTCCCAGCT GCCAGCCCTG CCAGTGTAAT GGTCATGCTC TAGACTGTGA CACAGTGACA 
GGGGAGTGTC TGAGCTGTCA GGACTACACC ACGGGCCACA ACTGCGAAAG GTGCCTGGCT 
GGCTACTACG GTGATCCCAT CATTGGGTCA GGAGACCACT GTCGCCCTTG CCCTTGTCCT 
GATGGTCCTG ACAGTGGACG ACAGTTTGCC AGGAGCTGTT ATCAAGACCC CGTCACTCTC 
CAGCTTGCGT GTGTTTGTGA TCCTGGGTAC ATTGGCTCCA GATGTGATGA CTGTGCCTCT 
GGATTTTTTG GCAATCCCTC AGACTTTGGG GGTTCATGTC AACCATGTCA GTGCCACCAC 
AACATTGACA CTACCGATCC AGAAGCCTGT GACAAGGAGA CGGGACGATG CCTCAAGTGC 
CTGTACCACA CAGAAGGGGA CCATTGCCAG CTCTGCCAGT ATGGGTACTA CGGCGATGCT 
CTTCGGCAAG ACTGTAGAAA GTGTGTCTGC AATTACCTGG GCACGGTGAA GGAACATTGT 
AATGGCTCTG ACTGCCACTG TGACAAAGCC ACTGGTCAGT GCTCGTGCCT TCCCAATGTG 
ATCGGGCAGA ACTGTGACCG GTGTGCGCCC AACACCTGGC AGCTGGCTAG CGGGACTGGC 
TGCGGGCCCT GCAATTGCAA TGCTGCGCAT TCCTTTGGGC CATCCTGCAA CGAGTTCACA 
GGGCAGTGCC AGTGCATGCC GGGCTTTGGA GGCCGAACCT GCAGCGAGTG CCAGGAGCTC 
TTCTGGGGAG ACCCTGATGT GGAATGCCGA GCCTGTGACT GTGATCCCAG GGGCATTGAG 
ACACCTCAGT GTGACCAGTC CACGGGCCAG TGTGTCTGTG TGGAGGGTGT AGAGGGTCCT 
CGCTGCGACA AGTGCACCAG AGGTTACTCG GGGGTCTTTC CTGACTGCAC ACCCTGCCAC 
CAGTGCTTTG CTCTCTGGGA TGCTATCATT GGTGAGCTGA CCAACAGGAC CCACAAATTC 
CTGGAGAAAG CCAAGGCTCT GAAAATCAGT GGTGTGATTG GTCCCTACCG AGAGACCGTG 
GACTCTGTAG AGAAGAAAGT CAATGAGATA AAAGACATCC TGGCCCAGAG CCCAGCAGCG 
GAACCACTGA AAAACATTGG CATTCTCTTC GAGGAGGCAG AGAAACTAAC CAAAGATGTC 
ACAGAAAAGA TGGCGCAGGT AGAAGTGAAA TTAACTGATA CAGCTTCACA GAGTAACAGC 
ACAGCTGGAG AGCTCGGCGC ACTGCAGGCA GAAGCAGAGA GCCTTGACAA GACCGTGAAG 
GAGCTGGCAG AACAGCTGGA GTTTATCAAA AACTCCGATA TTCAGGGCGC CTTGGATAGC 
ATCACCAAGT ATTTCCAGAT GTCTCTTGAG GCAGAGAAGC GGGTGAATGC CTCCACCACA 
GACCCCAACA GCACTGTGGA GCAGTCTGCC CTCACGCGAG ACAGAGTAGA AGATCTGATG 
TTGGAGCGAG AGTCTCCGTT CAAGGAGCAG CAGGAGGAAC AGGCACGCCT CCTGGACGAA 
CTGGCCGGCA AACTGCAAAG TCTCGACCTG TCGGCTGTTG CACAGATGAC CTGTGGAACA 
CCTCCAGGGG CTGACTGTTC TGAAAGTGAA TGTGGTGGCC CCAACTGCAG AACTGACGAA 
GGAGAGAAGA AGTGTGGGGG GCCTGGCTGT GGTGGTCTGG TCACTGTGGC CCACAGTGCT 
TGGCAGAAAG CCATGGATTT TGACCGTGAT GTCCTGAGTG CCCTGGCTGA AGTCGAACAG 
CTCTCCAAGA TGGTCTCTGA AGCAAAAGTG AGAGCAGATG AGGCGAAGCA GAATGCGCAG 
GATGTCCTGT TAAAAACAAA TGCTACCAAA GAAAAAGTGG ACAAGAGCAA CGAGGACCTG 
CGGAACCTCA TCAAGCAGAT CAGAAACTTC CTGACTGAGG ATAGTGCTGA TCTAGACAGT 
ATTGAAGCAG TTGCTAATGA AGTACTGAAA ATGGAAATGC CTAGCACGCC ACAGCAGTTA 
CAGAACCTAA CAGAAGACAT TCGGGAGCGA GTTGAAACCC TCTCTCAAGT AGAGGTTATT 
TTGCAGCAGA GTGCAGCTGA CATTGCCAGA GCTGAGCTGT TGCTTGAGGA AGCTAAGAGA 
GCAAGCAAAA GTGCAACAGA TGTTAAAGTC ACTGCAGACA TGGTGAAGGA AGCATTAGAA 
GAAGCAGAAA AGGCCCAGGT TGCAGCAGAG AAGGCGATTA AACAAGCTGA TGAGGATATC 
CAAGGAACCC AAAACCTGCT AACATCGATT GAATCTGAAA CGGCAGCTTC TGAGGAAACC 
CTGACCAACG CCTCCCAGCG CATCAGCAAG CTTGAGAGGA ACGTGGAAGA GCTTAAGCGT 
AAAGCTGCCC AGAACTCTGG GGAGGCAGAA TATATCGAAA AAGTAGTATA TTCTGTAAAA 
CAGAATGCAG ACGATGTTAA GAAGACTCTA GATGGCGAAC TTGATGAAAA GTATAAGAAG 
GTAGAAAGTT TAATTGCCCA AAAAACTGAA GAGTCAGCAG ATGCCAGGAG GAAAGCTGAG 
CTGCTACAAA ATGAAGCAAA AACACTCTTG GCTCAAGCTA ACAGCAAGCT CCAGCTGTTG 
GAAGACTTAG AAAGAAAATA TGAGGACAAT CAAAAATACT TAGAAGATAA AGCTCAAGAA 
TTGGTGCGAC TGGAAGGAGA GGTTCGCTCC CTCCTTAAGG ACATAAGTGA GAAAGTTGCG 
GTTTACAGCA CCTGCTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lamb1 ( XM_006514993.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515056.1 Mus musculus laminin B1 (Lamb1), transcript variant X2, mRNA.

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CloneID	OMu47246
Clone ID Related Accession (Same CDS sequence)	XM_006514993.1 , NM_008482.3 , XM_006514992.3
Accession Version	XM_006514993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	laminin subunit beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGC TCCAGGTGTT CGCCTTTGGT GTCCTAGCCC TATGGGGCAC CCGAGTGTGC 
GCTCAGGAAC CGGAGTTCAG CTATGGCTGC GCAGAAGGCA GCTGCTACCC TGCCACTGGC 
GACCTTCTCA TCGGCCGAGC GCAAAAGCTC TCCGTGACTT CGACATGTGG ACTGCACAAA 
CCAGAGCCCT ACTGTATTGT TAGCCACCTG CAGGAGGACA AGAAATGCTT CATATGTGAC 
TCCCGAGACC CTTATCACGA GACCCTCAAC CCCGACAGCC ATCTCATTGA GAACGTGGTC 
ACCACATTTG CTCCAAACCG CCTTAAGATC TGGTGGCAAT CGGAAAATGG TGTGGAGAAC 
GTGACCATCC AACTGGACCT GGAAGCAGAA TTCCATTTCA CTCATCTCAT CATGACCTTC 
AAGACATTCC GCCCAGCCGC CATGCTGATC GAGCGGTCTT CTGACTTTGG GAAGGCTTGG 
GGCGTGTACA GATACTTCGC CTACGACTGT GAGAGCTCGT TCCCAGGCAT TTCAACTGGA 
CCCATGAAGA AAGTGGATGA CATCATCTGT GACTCTCGAT ATTCTGACAT TGAGCCCTCG 
ACAGAAGGAG AGGTAATATT TCGTGCTTTA GATCCTGCTT TCAAAATTGA AGACCCTTAT 
AGTCCAAGGA TACAGAATCT ATTAAAAATC ACCAACTTGA GAATCAAGTT TGTGAAACTG 
CACACCTTGG GGGATAACCT TTTGGACTCC AGAATGGAAA TCCGAGAGAA GTACTATTAC 
GCTGTTTATG ATATGGTGGT TCGAGGGAAC TGCTTCTGCT ATGGCCACGC CAGTGAATGC 
GCCCCTGTGG ATGGAGTCAA TGAAGAAGTG GAAGGAATGG TTCACGGGCA CTGCATGTGC 
AGACACAACA CCAAAGGCCT GAACTGTGAG CTGTGCATGG ATTTCTACCA CGATTTGCCG 
TGGAGACCTG CTGAAGGCCG GAACAGCAAC GCCTGCAAAA AATGTAACTG CAATGAACAT 
TCCAGCTCGT GTCACTTTGA CATGGCAGTC TTCCTGGCTA CTGGCAACGT CAGCGGGGGA 
GTGTGTGATA ACTGTCAGCA CAACACCATG GGGCGCAACT GTGAACAGTG CAAACCGTTC 
TACTTCCAGC ACCCTGAGAG GGACATCCGG GACCCCAATC TCTGTGAACC ATGTACCTGT 
GACCCAGCTG GTTCTGAGAA TGGCGGGATC TGTGATGGGT ACACTGATTT TTCTGTGGGT 
CTCATTGCTG GTCAGTGTCG GTGCAAATTG CACGTGGAGG GAGAGCGCTG TGATGTTTGT 
AAAGAAGGCT TCTACGACTT AAGTGCTGAA GACCCGTATG GTTGTAAATC ATGTGCTTGC 
AATCCTCTGG GAACAATTCC TGGTGGGAAT CCTTGTGATT CTGAGACTGG CTACTGCTAC 
TGTAAGCGCC TGGTGACAGG ACAGCGCTGT GACCAGTGCC TGCCGCAGCA CTGGGGTTTA 
AGCAATGATT TGGATGGGTG TCGACCTTGT GACTGTGACC TTGGAGGGGC GCTGAACAAT 
AGCTGCTCCG AGGACTCCGG CCAGTGCTCC TGCCTGCCCC ACATGATTGG GCGGCAGTGT 
AACGAGGTGG AGTCCGGTTA CTACTTCACC ACCCTGGACC ACTACATCTA CGAAGCCGAG 
GAAGCCAATC TGGGGCCTGG AGTCATTGTG GTGGAAAGGC AGTACATTCA GGACCGCATT 
CCTTCCTGGA CAGGACCTGG GTTCGTCCGG GTGCCTGAAG GGGCTTATTT GGAGTTTTTC 
ATTGACAACA TACCGTATTC CATGGAGTAT GAAATCCTGA TTCGCTATGA GCCACAGCTG 
CCGGACCACT GGGAGAAAGC TGTCATCACT GTACAGCGGC CGGGGAAGAT TCCAGCCAGC 
AGCCGATGTG GTAACACCGT TCCCGATGAT GACAACCAGG TGGTGTCCTT GTCACCGGGC 
TCAAGATACG TTGTCCTCCC TCGCCCCGTG TGCTTTGAGA AGGGAATGAA CTACACGGTG 
AGGTTGGAGC TGCCCCAGTA TACGGCATCG GGCAGTGACG TGGAGAGCCC TTACACGTTC 
ATCGACTCGC TTGTTCTCAT GCCCTACTGT AAATCGCTGG ACATCTTCAC TGTTGGCGGC 
TCAGGCGATG GGGAGGTCAC CAATAGTGCC TGGGAAACCT TCCAGCGCTA CAGGTGTCTG 
GAGAACAGCA GGAGTGTGGT AAAAACACCC ATGACAGATG TCTGCAGAAA CATTATCTTC 
AGCATTTCTG CCTTGATTCA CCAGACGGGC CTTGCTTGTG AATGTGACCC CCAGGGATCT 
CTGAGTTCTG TGTGTGACCC CAATGGTGGC CAGTGCCAGT GCCGTCCTAA TGTGGTTGGA 
AGAACCTGCA ACAGGTGTGC CCCGGGCACC TTTGGCTTTG GCCCCAACGG ATGCAAACCT 
TGTGACTGCC ATCTGCAAGG GTCTGCCAGT GCCTTCTGCG ATGCGATCAC TGGCCAGTGC 
CACTGTTTCC AGGGCATCTA TGCTCGGCAG TGTGACCGAT GTCTCCCTGG GTATTGGGGC 
TTTCCCAGCT GCCAGCCCTG CCAGTGTAAT GGTCATGCTC TAGACTGTGA CACAGTGACA 
GGGGAGTGTC TGAGCTGTCA GGACTACACC ACGGGCCACA ACTGCGAAAG GTGCCTGGCT 
GGCTACTACG GTGATCCCAT CATTGGGTCA GGAGACCACT GTCGCCCTTG CCCTTGTCCT 
GATGGTCCTG ACAGTGGACG ACAGTTTGCC AGGAGCTGTT ATCAAGACCC CGTCACTCTC 
CAGCTTGCGT GTGTTTGTGA TCCTGGGTAC ATTGGCTCCA GATGTGATGA CTGTGCCTCT 
GGATTTTTTG GCAATCCCTC AGACTTTGGG GGTTCATGTC AACCATGTCA GTGCCACCAC 
AACATTGACA CTACCGATCC AGAAGCCTGT GACAAGGAGA CGGGACGATG CCTCAAGTGC 
CTGTACCACA CAGAAGGGGA CCATTGCCAG CTCTGCCAGT ATGGGTACTA CGGCGATGCT 
CTTCGGCAAG ACTGTAGAAA GTGTGTCTGC AATTACCTGG GCACGGTGAA GGAACATTGT 
AATGGCTCTG ACTGCCACTG TGACAAAGCC ACTGGTCAGT GCTCGTGCCT TCCCAATGTG 
ATCGGGCAGA ACTGTGACCG GTGTGCGCCC AACACCTGGC AGCTGGCTAG CGGGACTGGC 
TGCGGGCCCT GCAATTGCAA TGCTGCGCAT TCCTTTGGGC CATCCTGCAA CGAGTTCACA 
GGGCAGTGCC AGTGCATGCC GGGCTTTGGA GGCCGAACCT GCAGCGAGTG CCAGGAGCTC 
TTCTGGGGAG ACCCTGATGT GGAATGCCGA GCCTGTGACT GTGATCCCAG GGGCATTGAG 
ACACCTCAGT GTGACCAGTC CACGGGCCAG TGTGTCTGTG TGGAGGGTGT AGAGGGTCCT 
CGCTGCGACA AGTGCACCAG AGGTTACTCG GGGGTCTTTC CTGACTGCAC ACCCTGCCAC 
CAGTGCTTTG CTCTCTGGGA TGCTATCATT GGTGAGCTGA CCAACAGGAC CCACAAATTC 
CTGGAGAAAG CCAAGGCTCT GAAAATCAGT GGTGTGATTG GTCCCTACCG AGAGACCGTG 
GACTCTGTAG AGAAGAAAGT CAATGAGATA AAAGACATCC TGGCCCAGAG CCCAGCAGCG 
GAACCACTGA AAAACATTGG CATTCTCTTC GAGGAGGCAG AGAAACTAAC CAAAGATGTC 
ACAGAAAAGA TGGCGCAGGT AGAAGTGAAA TTAACTGATA CAGCTTCACA GAGTAACAGC 
ACAGCTGGAG AGCTCGGCGC ACTGCAGGCA GAAGCAGAGA GCCTTGACAA GACCGTGAAG 
GAGCTGGCAG AACAGCTGGA GTTTATCAAA AACTCCGATA TTCAGGGCGC CTTGGATAGC 
ATCACCAAGT ATTTCCAGAT GTCTCTTGAG GCAGAGAAGC GGGTGAATGC CTCCACCACA 
GACCCCAACA GCACTGTGGA GCAGTCTGCC CTCACGCGAG ACAGAGTAGA AGATCTGATG 
TTGGAGCGAG AGTCTCCGTT CAAGGAGCAG CAGGAGGAAC AGGCACGCCT CCTGGACGAA 
CTGGCCGGCA AACTGCAAAG TCTCGACCTG TCGGCTGTTG CACAGATGAC CTGTGGAACA 
CCTCCAGGGG CTGACTGTTC TGAAAGTGAA TGTGGTGGCC CCAACTGCAG AACTGACGAA 
GGAGAGAAGA AGTGTGGGGG GCCTGGCTGT GGTGGTCTGG TCACTGTGGC CCACAGTGCT 
TGGCAGAAAG CCATGGATTT TGACCGTGAT GTCCTGAGTG CCCTGGCTGA AGTCGAACAG 
CTCTCCAAGA TGGTCTCTGA AGCAAAAGTG AGAGCAGATG AGGCGAAGCA GAATGCGCAG 
GATGTCCTGT TAAAAACAAA TGCTACCAAA GAAAAAGTGG ACAAGAGCAA CGAGGACCTG 
CGGAACCTCA TCAAGCAGAT CAGAAACTTC CTGACTGAGG ATAGTGCTGA TCTAGACAGT 
ATTGAAGCAG TTGCTAATGA AGTACTGAAA ATGGAAATGC CTAGCACGCC ACAGCAGTTA 
CAGAACCTAA CAGAAGACAT TCGGGAGCGA GTTGAAACCC TCTCTCAAGT AGAGGTTATT 
TTGCAGCAGA GTGCAGCTGA CATTGCCAGA GCTGAGCTGT TGCTTGAGGA AGCTAAGAGA 
GCAAGCAAAA GTGCAACAGA TGTTAAAGTC ACTGCAGACA TGGTGAAGGA AGCATTAGAA 
GAAGCAGAAA AGGCCCAGGT TGCAGCAGAG AAGGCGATTA AACAAGCTGA TGAGGATATC 
CAAGGAACCC AAAACCTGCT AACATCGATT GAATCTGAAA CGGCAGCTTC TGAGGAAACC 
CTGACCAACG CCTCCCAGCG CATCAGCAAG CTTGAGAGGA ACGTGGAAGA GCTTAAGCGT 
AAAGCTGCCC AGAACTCTGG GGAGGCAGAA TATATCGAAA AAGTAGTATA TTCTGTAAAA 
CAGAATGCAG ACGATGTTAA GAAGACTCTA GATGGCGAAC TTGATGAAAA GTATAAGAAG 
GTAGAAAGTT TAATTGCCCA AAAAACTGAA GAGTCAGCAG ATGCCAGGAG GAAAGCTGAG 
CTGCTACAAA ATGAAGCAAA AACACTCTTG GCTCAAGCTA ACAGCAAGCT CCAGCTGTTG 
GAAGACTTAG AAAGAAAATA TGAGGACAAT CAAAAATACT TAGAAGATAA AGCTCAAGAA 
TTGGTGCGAC TGGAAGGAGA GGTTCGCTCC CTCCTTAAGG ACATAAGTGA GAAAGTTGCG 
GTTTACAGCA CCTGCTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515056.1
CDS	192..5552
Translation	MGLLQVFAFGVLALWGTRVCAQEPEFSYGCAEGSCYPATGDLLIGRAQKLSVTSTCGLHKPEPYCIVSHLQEDKKCFICDSRDPYHETLNPDSHLIENVVTTFAPNRLKIWWQSENGVENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKAWGVYRYFAYDCESSFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIFRALDPAFKIEDPYSPRIQNLLKITNLRIKFVKLHTLGDNLLDSRMEIREKYYYAVYDMVVRGNCFCYGHASECAPVDGVNEEVEGMVHGHCMCRHNTKGLNCELCMDFYHDLPWRPAEGRNSNACKKCNCNEHSSSCHFDMAVFLATGNVSGGVCDNCQHNTMGRNCEQCKPFYFQHPERDIRDPNLCEPCTCDPAGSENGGICDGYTDFSVGLIAGQCRCKLHVEGERCDVCKEGFYDLSAEDPYGCKSCACNPLGTIPGGNPCDSETGYCYCKRLVTGQRCDQCLPQHWGLSNDLDGCRPCDCDLGGALNNSCSEDSGQCSCLPHMIGRQCNEVESGYYFTTLDHYIYEAEEANLGPGVIVVERQYIQDRIPSWTGPGFVRVPEGAYLEFFIDNIPYSMEYEILIRYEPQLPDHWEKAVITVQRPGKIPASSRCGNTVPDDDNQVVSLSPGSRYVVLPRPVCFEKGMNYTVRLELPQYTASGSDVESPYTFIDSLVLMPYCKSLDIFTVGGSGDGEVTNSAWETFQRYRCLENSRSVVKTPMTDVCRNIIFSISALIHQTGLACECDPQGSLSSVCDPNGGQCQCRPNVVGRTCNRCAPGTFGFGPNGCKPCDCHLQGSASAFCDAITGQCHCFQGIYARQCDRCLPGYWGFPSCQPCQCNGHALDCDTVTGECLSCQDYTTGHNCERCLAGYYGDPIIGSGDHCRPCPCPDGPDSGRQFARSCYQDPVTLQLACVCDPGYIGSRCDDCASGFFGNPSDFGGSCQPCQCHHNIDTTDPEACDKETGRCLKCLYHTEGDHCQLCQYGYYGDALRQDCRKCVCNYLGTVKEHCNGSDCHCDKATGQCSCLPNVIGQNCDRCAPNTWQLASGTGCGPCNCNAAHSFGPSCNEFTGQCQCMPGFGGRTCSECQELFWGDPDVECRACDCDPRGIETPQCDQSTGQCVCVEGVEGPRCDKCTRGYSGVFPDCTPCHQCFALWDAIIGELTNRTHKFLEKAKALKISGVIGPYRETVDSVEKKVNEIKDILAQSPAAEPLKNIGILFEEAEKLTKDVTEKMAQVEVKLTDTASQSNSTAGELGALQAEAESLDKTVKELAEQLEFIKNSDIQGALDSITKYFQMSLEAEKRVNASTTDPNSTVEQSALTRDRVEDLMLERESPFKEQQEEQARLLDELAGKLQSLDLSAVAQMTCGTPPGADCSESECGGPNCRTDEGEKKCGGPGCGGLVTVAHSAWQKAMDFDRDVLSALAEVEQLSKMVSEAKVRADEAKQNAQDVLLKTNATKEKVDKSNEDLRNLIKQIRNFLTEDSADLDSIEAVANEVLKMEMPSTPQQLQNLTEDIRERVETLSQVEVILQQSAADIARAELLLEEAKRASKSATDVKVTADMVKEALEEAEKAQVAAEKAIKQADEDIQGTQNLLTSIESETAASEETLTNASQRISKLERNVEELKRKAAQNSGEAEYIEKVVYSVKQNADDVKKTLDGELDEKYKKVESLIAQKTEESADARRKAELLQNEAKTLLAQANSKLQLLEDLERKYEDNQKYLEDKAQELVRLEGEVRSLLKDISEKVAVYSTCL

Target ORF information:

RefSeq Version	XM_006514993.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus laminin B1 (Lamb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514993.1

ORF Insert Sequence:

ATGGGGCTGC TCCAGGTGTT CGCCTTTGGT GTCCTAGCCC TATGGGGCAC CCGAGTGTGC 
GCTCAGGAAC CGGAGTTCAG CTATGGCTGC GCAGAAGGCA GCTGCTACCC TGCCACTGGC 
GACCTTCTCA TCGGCCGAGC GCAAAAGCTC TCCGTGACTT CGACATGTGG ACTGCACAAA 
CCAGAGCCCT ACTGTATTGT TAGCCACCTG CAGGAGGACA AGAAATGCTT CATATGTGAC 
TCCCGAGACC CTTATCACGA GACCCTCAAC CCCGACAGCC ATCTCATTGA GAACGTGGTC 
ACCACATTTG CTCCAAACCG CCTTAAGATC TGGTGGCAAT CGGAAAATGG TGTGGAGAAC 
GTGACCATCC AACTGGACCT GGAAGCAGAA TTCCATTTCA CTCATCTCAT CATGACCTTC 
AAGACATTCC GCCCAGCCGC CATGCTGATC GAGCGGTCTT CTGACTTTGG GAAGGCTTGG 
GGCGTGTACA GATACTTCGC CTACGACTGT GAGAGCTCGT TCCCAGGCAT TTCAACTGGA 
CCCATGAAGA AAGTGGATGA CATCATCTGT GACTCTCGAT ATTCTGACAT TGAGCCCTCG 
ACAGAAGGAG AGGTAATATT TCGTGCTTTA GATCCTGCTT TCAAAATTGA AGACCCTTAT 
AGTCCAAGGA TACAGAATCT ATTAAAAATC ACCAACTTGA GAATCAAGTT TGTGAAACTG 
CACACCTTGG GGGATAACCT TTTGGACTCC AGAATGGAAA TCCGAGAGAA GTACTATTAC 
GCTGTTTATG ATATGGTGGT TCGAGGGAAC TGCTTCTGCT ATGGCCACGC CAGTGAATGC 
GCCCCTGTGG ATGGAGTCAA TGAAGAAGTG GAAGGAATGG TTCACGGGCA CTGCATGTGC 
AGACACAACA CCAAAGGCCT GAACTGTGAG CTGTGCATGG ATTTCTACCA CGATTTGCCG 
TGGAGACCTG CTGAAGGCCG GAACAGCAAC GCCTGCAAAA AATGTAACTG CAATGAACAT 
TCCAGCTCGT GTCACTTTGA CATGGCAGTC TTCCTGGCTA CTGGCAACGT CAGCGGGGGA 
GTGTGTGATA ACTGTCAGCA CAACACCATG GGGCGCAACT GTGAACAGTG CAAACCGTTC 
TACTTCCAGC ACCCTGAGAG GGACATCCGG GACCCCAATC TCTGTGAACC ATGTACCTGT 
GACCCAGCTG GTTCTGAGAA TGGCGGGATC TGTGATGGGT ACACTGATTT TTCTGTGGGT 
CTCATTGCTG GTCAGTGTCG GTGCAAATTG CACGTGGAGG GAGAGCGCTG TGATGTTTGT 
AAAGAAGGCT TCTACGACTT AAGTGCTGAA GACCCGTATG GTTGTAAATC ATGTGCTTGC 
AATCCTCTGG GAACAATTCC TGGTGGGAAT CCTTGTGATT CTGAGACTGG CTACTGCTAC 
TGTAAGCGCC TGGTGACAGG ACAGCGCTGT GACCAGTGCC TGCCGCAGCA CTGGGGTTTA 
AGCAATGATT TGGATGGGTG TCGACCTTGT GACTGTGACC TTGGAGGGGC GCTGAACAAT 
AGCTGCTCCG AGGACTCCGG CCAGTGCTCC TGCCTGCCCC ACATGATTGG GCGGCAGTGT 
AACGAGGTGG AGTCCGGTTA CTACTTCACC ACCCTGGACC ACTACATCTA CGAAGCCGAG 
GAAGCCAATC TGGGGCCTGG AGTCATTGTG GTGGAAAGGC AGTACATTCA GGACCGCATT 
CCTTCCTGGA CAGGACCTGG GTTCGTCCGG GTGCCTGAAG GGGCTTATTT GGAGTTTTTC 
ATTGACAACA TACCGTATTC CATGGAGTAT GAAATCCTGA TTCGCTATGA GCCACAGCTG 
CCGGACCACT GGGAGAAAGC TGTCATCACT GTACAGCGGC CGGGGAAGAT TCCAGCCAGC 
AGCCGATGTG GTAACACCGT TCCCGATGAT GACAACCAGG TGGTGTCCTT GTCACCGGGC 
TCAAGATACG TTGTCCTCCC TCGCCCCGTG TGCTTTGAGA AGGGAATGAA CTACACGGTG 
AGGTTGGAGC TGCCCCAGTA TACGGCATCG GGCAGTGACG TGGAGAGCCC TTACACGTTC 
ATCGACTCGC TTGTTCTCAT GCCCTACTGT AAATCGCTGG ACATCTTCAC TGTTGGCGGC 
TCAGGCGATG GGGAGGTCAC CAATAGTGCC TGGGAAACCT TCCAGCGCTA CAGGTGTCTG 
GAGAACAGCA GGAGTGTGGT AAAAACACCC ATGACAGATG TCTGCAGAAA CATTATCTTC 
AGCATTTCTG CCTTGATTCA CCAGACGGGC CTTGCTTGTG AATGTGACCC CCAGGGATCT 
CTGAGTTCTG TGTGTGACCC CAATGGTGGC CAGTGCCAGT GCCGTCCTAA TGTGGTTGGA 
AGAACCTGCA ACAGGTGTGC CCCGGGCACC TTTGGCTTTG GCCCCAACGG ATGCAAACCT 
TGTGACTGCC ATCTGCAAGG GTCTGCCAGT GCCTTCTGCG ATGCGATCAC TGGCCAGTGC 
CACTGTTTCC AGGGCATCTA TGCTCGGCAG TGTGACCGAT GTCTCCCTGG GTATTGGGGC 
TTTCCCAGCT GCCAGCCCTG CCAGTGTAAT GGTCATGCTC TAGACTGTGA CACAGTGACA 
GGGGAGTGTC TGAGCTGTCA GGACTACACC ACGGGCCACA ACTGCGAAAG GTGCCTGGCT 
GGCTACTACG GTGATCCCAT CATTGGGTCA GGAGACCACT GTCGCCCTTG CCCTTGTCCT 
GATGGTCCTG ACAGTGGACG ACAGTTTGCC AGGAGCTGTT ATCAAGACCC CGTCACTCTC 
CAGCTTGCGT GTGTTTGTGA TCCTGGGTAC ATTGGCTCCA GATGTGATGA CTGTGCCTCT 
GGATTTTTTG GCAATCCCTC AGACTTTGGG GGTTCATGTC AACCATGTCA GTGCCACCAC 
AACATTGACA CTACCGATCC AGAAGCCTGT GACAAGGAGA CGGGACGATG CCTCAAGTGC 
CTGTACCACA CAGAAGGGGA CCATTGCCAG CTCTGCCAGT ATGGGTACTA CGGCGATGCT 
CTTCGGCAAG ACTGTAGAAA GTGTGTCTGC AATTACCTGG GCACGGTGAA GGAACATTGT 
AATGGCTCTG ACTGCCACTG TGACAAAGCC ACTGGTCAGT GCTCGTGCCT TCCCAATGTG 
ATCGGGCAGA ACTGTGACCG GTGTGCGCCC AACACCTGGC AGCTGGCTAG CGGGACTGGC 
TGCGGGCCCT GCAATTGCAA TGCTGCGCAT TCCTTTGGGC CATCCTGCAA CGAGTTCACA 
GGGCAGTGCC AGTGCATGCC GGGCTTTGGA GGCCGAACCT GCAGCGAGTG CCAGGAGCTC 
TTCTGGGGAG ACCCTGATGT GGAATGCCGA GCCTGTGACT GTGATCCCAG GGGCATTGAG 
ACACCTCAGT GTGACCAGTC CACGGGCCAG TGTGTCTGTG TGGAGGGTGT AGAGGGTCCT 
CGCTGCGACA AGTGCACCAG AGGTTACTCG GGGGTCTTTC CTGACTGCAC ACCCTGCCAC 
CAGTGCTTTG CTCTCTGGGA TGCTATCATT GGTGAGCTGA CCAACAGGAC CCACAAATTC 
CTGGAGAAAG CCAAGGCTCT GAAAATCAGT GGTGTGATTG GTCCCTACCG AGAGACCGTG 
GACTCTGTAG AGAAGAAAGT CAATGAGATA AAAGACATCC TGGCCCAGAG CCCAGCAGCG 
GAACCACTGA AAAACATTGG CATTCTCTTC GAGGAGGCAG AGAAACTAAC CAAAGATGTC 
ACAGAAAAGA TGGCGCAGGT AGAAGTGAAA TTAACTGATA CAGCTTCACA GAGTAACAGC 
ACAGCTGGAG AGCTCGGCGC ACTGCAGGCA GAAGCAGAGA GCCTTGACAA GACCGTGAAG 
GAGCTGGCAG AACAGCTGGA GTTTATCAAA AACTCCGATA TTCAGGGCGC CTTGGATAGC 
ATCACCAAGT ATTTCCAGAT GTCTCTTGAG GCAGAGAAGC GGGTGAATGC CTCCACCACA 
GACCCCAACA GCACTGTGGA GCAGTCTGCC CTCACGCGAG ACAGAGTAGA AGATCTGATG 
TTGGAGCGAG AGTCTCCGTT CAAGGAGCAG CAGGAGGAAC AGGCACGCCT CCTGGACGAA 
CTGGCCGGCA AACTGCAAAG TCTCGACCTG TCGGCTGTTG CACAGATGAC CTGTGGAACA 
CCTCCAGGGG CTGACTGTTC TGAAAGTGAA TGTGGTGGCC CCAACTGCAG AACTGACGAA 
GGAGAGAAGA AGTGTGGGGG GCCTGGCTGT GGTGGTCTGG TCACTGTGGC CCACAGTGCT 
TGGCAGAAAG CCATGGATTT TGACCGTGAT GTCCTGAGTG CCCTGGCTGA AGTCGAACAG 
CTCTCCAAGA TGGTCTCTGA AGCAAAAGTG AGAGCAGATG AGGCGAAGCA GAATGCGCAG 
GATGTCCTGT TAAAAACAAA TGCTACCAAA GAAAAAGTGG ACAAGAGCAA CGAGGACCTG 
CGGAACCTCA TCAAGCAGAT CAGAAACTTC CTGACTGAGG ATAGTGCTGA TCTAGACAGT 
ATTGAAGCAG TTGCTAATGA AGTACTGAAA ATGGAAATGC CTAGCACGCC ACAGCAGTTA 
CAGAACCTAA CAGAAGACAT TCGGGAGCGA GTTGAAACCC TCTCTCAAGT AGAGGTTATT 
TTGCAGCAGA GTGCAGCTGA CATTGCCAGA GCTGAGCTGT TGCTTGAGGA AGCTAAGAGA 
GCAAGCAAAA GTGCAACAGA TGTTAAAGTC ACTGCAGACA TGGTGAAGGA AGCATTAGAA 
GAAGCAGAAA AGGCCCAGGT TGCAGCAGAG AAGGCGATTA AACAAGCTGA TGAGGATATC 
CAAGGAACCC AAAACCTGCT AACATCGATT GAATCTGAAA CGGCAGCTTC TGAGGAAACC 
CTGACCAACG CCTCCCAGCG CATCAGCAAG CTTGAGAGGA ACGTGGAAGA GCTTAAGCGT 
AAAGCTGCCC AGAACTCTGG GGAGGCAGAA TATATCGAAA AAGTAGTATA TTCTGTAAAA 
CAGAATGCAG ACGATGTTAA GAAGACTCTA GATGGCGAAC TTGATGAAAA GTATAAGAAG 
GTAGAAAGTT TAATTGCCCA AAAAACTGAA GAGTCAGCAG ATGCCAGGAG GAAAGCTGAG 
CTGCTACAAA ATGAAGCAAA AACACTCTTG GCTCAAGCTA ACAGCAAGCT CCAGCTGTTG 
GAAGACTTAG AAAGAAAATA TGAGGACAAT CAAAAATACT TAGAAGATAA AGCTCAAGAA 
TTGGTGCGAC TGGAAGGAGA GGTTCGCTCC CTCCTTAAGG ACATAAGTGA GAAAGTTGCG 
GTTTACAGCA CCTGCTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lamb1 ( XM_006514992.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515055.2 Mus musculus laminin B1 (Lamb1), transcript variant X1, mRNA.

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CloneID	OMu47246
Clone ID Related Accession (Same CDS sequence)	XM_006514993.1 , NM_008482.3 , XM_006514992.3
Accession Version	XM_006514992.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	laminin subunit beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514992.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGC TCCAGGTGTT CGCCTTTGGT GTCCTAGCCC TATGGGGCAC CCGAGTGTGC 
GCTCAGGAAC CGGAGTTCAG CTATGGCTGC GCAGAAGGCA GCTGCTACCC TGCCACTGGC 
GACCTTCTCA TCGGCCGAGC GCAAAAGCTC TCCGTGACTT CGACATGTGG ACTGCACAAA 
CCAGAGCCCT ACTGTATTGT TAGCCACCTG CAGGAGGACA AGAAATGCTT CATATGTGAC 
TCCCGAGACC CTTATCACGA GACCCTCAAC CCCGACAGCC ATCTCATTGA GAACGTGGTC 
ACCACATTTG CTCCAAACCG CCTTAAGATC TGGTGGCAAT CGGAAAATGG TGTGGAGAAC 
GTGACCATCC AACTGGACCT GGAAGCAGAA TTCCATTTCA CTCATCTCAT CATGACCTTC 
AAGACATTCC GCCCAGCCGC CATGCTGATC GAGCGGTCTT CTGACTTTGG GAAGGCTTGG 
GGCGTGTACA GATACTTCGC CTACGACTGT GAGAGCTCGT TCCCAGGCAT TTCAACTGGA 
CCCATGAAGA AAGTGGATGA CATCATCTGT GACTCTCGAT ATTCTGACAT TGAGCCCTCG 
ACAGAAGGAG AGGTAATATT TCGTGCTTTA GATCCTGCTT TCAAAATTGA AGACCCTTAT 
AGTCCAAGGA TACAGAATCT ATTAAAAATC ACCAACTTGA GAATCAAGTT TGTGAAACTG 
CACACCTTGG GGGATAACCT TTTGGACTCC AGAATGGAAA TCCGAGAGAA GTACTATTAC 
GCTGTTTATG ATATGGTGGT TCGAGGGAAC TGCTTCTGCT ATGGCCACGC CAGTGAATGC 
GCCCCTGTGG ATGGAGTCAA TGAAGAAGTG GAAGGAATGG TTCACGGGCA CTGCATGTGC 
AGACACAACA CCAAAGGCCT GAACTGTGAG CTGTGCATGG ATTTCTACCA CGATTTGCCG 
TGGAGACCTG CTGAAGGCCG GAACAGCAAC GCCTGCAAAA AATGTAACTG CAATGAACAT 
TCCAGCTCGT GTCACTTTGA CATGGCAGTC TTCCTGGCTA CTGGCAACGT CAGCGGGGGA 
GTGTGTGATA ACTGTCAGCA CAACACCATG GGGCGCAACT GTGAACAGTG CAAACCGTTC 
TACTTCCAGC ACCCTGAGAG GGACATCCGG GACCCCAATC TCTGTGAACC ATGTACCTGT 
GACCCAGCTG GTTCTGAGAA TGGCGGGATC TGTGATGGGT ACACTGATTT TTCTGTGGGT 
CTCATTGCTG GTCAGTGTCG GTGCAAATTG CACGTGGAGG GAGAGCGCTG TGATGTTTGT 
AAAGAAGGCT TCTACGACTT AAGTGCTGAA GACCCGTATG GTTGTAAATC ATGTGCTTGC 
AATCCTCTGG GAACAATTCC TGGTGGGAAT CCTTGTGATT CTGAGACTGG CTACTGCTAC 
TGTAAGCGCC TGGTGACAGG ACAGCGCTGT GACCAGTGCC TGCCGCAGCA CTGGGGTTTA 
AGCAATGATT TGGATGGGTG TCGACCTTGT GACTGTGACC TTGGAGGGGC GCTGAACAAT 
AGCTGCTCCG AGGACTCCGG CCAGTGCTCC TGCCTGCCCC ACATGATTGG GCGGCAGTGT 
AACGAGGTGG AGTCCGGTTA CTACTTCACC ACCCTGGACC ACTACATCTA CGAAGCCGAG 
GAAGCCAATC TGGGGCCTGG AGTCATTGTG GTGGAAAGGC AGTACATTCA GGACCGCATT 
CCTTCCTGGA CAGGACCTGG GTTCGTCCGG GTGCCTGAAG GGGCTTATTT GGAGTTTTTC 
ATTGACAACA TACCGTATTC CATGGAGTAT GAAATCCTGA TTCGCTATGA GCCACAGCTG 
CCGGACCACT GGGAGAAAGC TGTCATCACT GTACAGCGGC CGGGGAAGAT TCCAGCCAGC 
AGCCGATGTG GTAACACCGT TCCCGATGAT GACAACCAGG TGGTGTCCTT GTCACCGGGC 
TCAAGATACG TTGTCCTCCC TCGCCCCGTG TGCTTTGAGA AGGGAATGAA CTACACGGTG 
AGGTTGGAGC TGCCCCAGTA TACGGCATCG GGCAGTGACG TGGAGAGCCC TTACACGTTC 
ATCGACTCGC TTGTTCTCAT GCCCTACTGT AAATCGCTGG ACATCTTCAC TGTTGGCGGC 
TCAGGCGATG GGGAGGTCAC CAATAGTGCC TGGGAAACCT TCCAGCGCTA CAGGTGTCTG 
GAGAACAGCA GGAGTGTGGT AAAAACACCC ATGACAGATG TCTGCAGAAA CATTATCTTC 
AGCATTTCTG CCTTGATTCA CCAGACGGGC CTTGCTTGTG AATGTGACCC CCAGGGATCT 
CTGAGTTCTG TGTGTGACCC CAATGGTGGC CAGTGCCAGT GCCGTCCTAA TGTGGTTGGA 
AGAACCTGCA ACAGGTGTGC CCCGGGCACC TTTGGCTTTG GCCCCAACGG ATGCAAACCT 
TGTGACTGCC ATCTGCAAGG GTCTGCCAGT GCCTTCTGCG ATGCGATCAC TGGCCAGTGC 
CACTGTTTCC AGGGCATCTA TGCTCGGCAG TGTGACCGAT GTCTCCCTGG GTATTGGGGC 
TTTCCCAGCT GCCAGCCCTG CCAGTGTAAT GGTCATGCTC TAGACTGTGA CACAGTGACA 
GGGGAGTGTC TGAGCTGTCA GGACTACACC ACGGGCCACA ACTGCGAAAG GTGCCTGGCT 
GGCTACTACG GTGATCCCAT CATTGGGTCA GGAGACCACT GTCGCCCTTG CCCTTGTCCT 
GATGGTCCTG ACAGTGGACG ACAGTTTGCC AGGAGCTGTT ATCAAGACCC CGTCACTCTC 
CAGCTTGCGT GTGTTTGTGA TCCTGGGTAC ATTGGCTCCA GATGTGATGA CTGTGCCTCT 
GGATTTTTTG GCAATCCCTC AGACTTTGGG GGTTCATGTC AACCATGTCA GTGCCACCAC 
AACATTGACA CTACCGATCC AGAAGCCTGT GACAAGGAGA CGGGACGATG CCTCAAGTGC 
CTGTACCACA CAGAAGGGGA CCATTGCCAG CTCTGCCAGT ATGGGTACTA CGGCGATGCT 
CTTCGGCAAG ACTGTAGAAA GTGTGTCTGC AATTACCTGG GCACGGTGAA GGAACATTGT 
AATGGCTCTG ACTGCCACTG TGACAAAGCC ACTGGTCAGT GCTCGTGCCT TCCCAATGTG 
ATCGGGCAGA ACTGTGACCG GTGTGCGCCC AACACCTGGC AGCTGGCTAG CGGGACTGGC 
TGCGGGCCCT GCAATTGCAA TGCTGCGCAT TCCTTTGGGC CATCCTGCAA CGAGTTCACA 
GGGCAGTGCC AGTGCATGCC GGGCTTTGGA GGCCGAACCT GCAGCGAGTG CCAGGAGCTC 
TTCTGGGGAG ACCCTGATGT GGAATGCCGA GCCTGTGACT GTGATCCCAG GGGCATTGAG 
ACACCTCAGT GTGACCAGTC CACGGGCCAG TGTGTCTGTG TGGAGGGTGT AGAGGGTCCT 
CGCTGCGACA AGTGCACCAG AGGTTACTCG GGGGTCTTTC CTGACTGCAC ACCCTGCCAC 
CAGTGCTTTG CTCTCTGGGA TGCTATCATT GGTGAGCTGA CCAACAGGAC CCACAAATTC 
CTGGAGAAAG CCAAGGCTCT GAAAATCAGT GGTGTGATTG GTCCCTACCG AGAGACCGTG 
GACTCTGTAG AGAAGAAAGT CAATGAGATA AAAGACATCC TGGCCCAGAG CCCAGCAGCG 
GAACCACTGA AAAACATTGG CATTCTCTTC GAGGAGGCAG AGAAACTAAC CAAAGATGTC 
ACAGAAAAGA TGGCGCAGGT AGAAGTGAAA TTAACTGATA CAGCTTCACA GAGTAACAGC 
ACAGCTGGAG AGCTCGGCGC ACTGCAGGCA GAAGCAGAGA GCCTTGACAA GACCGTGAAG 
GAGCTGGCAG AACAGCTGGA GTTTATCAAA AACTCCGATA TTCAGGGCGC CTTGGATAGC 
ATCACCAAGT ATTTCCAGAT GTCTCTTGAG GCAGAGAAGC GGGTGAATGC CTCCACCACA 
GACCCCAACA GCACTGTGGA GCAGTCTGCC CTCACGCGAG ACAGAGTAGA AGATCTGATG 
TTGGAGCGAG AGTCTCCGTT CAAGGAGCAG CAGGAGGAAC AGGCACGCCT CCTGGACGAA 
CTGGCCGGCA AACTGCAAAG TCTCGACCTG TCGGCTGTTG CACAGATGAC CTGTGGAACA 
CCTCCAGGGG CTGACTGTTC TGAAAGTGAA TGTGGTGGCC CCAACTGCAG AACTGACGAA 
GGAGAGAAGA AGTGTGGGGG GCCTGGCTGT GGTGGTCTGG TCACTGTGGC CCACAGTGCT 
TGGCAGAAAG CCATGGATTT TGACCGTGAT GTCCTGAGTG CCCTGGCTGA AGTCGAACAG 
CTCTCCAAGA TGGTCTCTGA AGCAAAAGTG AGAGCAGATG AGGCGAAGCA GAATGCGCAG 
GATGTCCTGT TAAAAACAAA TGCTACCAAA GAAAAAGTGG ACAAGAGCAA CGAGGACCTG 
CGGAACCTCA TCAAGCAGAT CAGAAACTTC CTGACTGAGG ATAGTGCTGA TCTAGACAGT 
ATTGAAGCAG TTGCTAATGA AGTACTGAAA ATGGAAATGC CTAGCACGCC ACAGCAGTTA 
CAGAACCTAA CAGAAGACAT TCGGGAGCGA GTTGAAACCC TCTCTCAAGT AGAGGTTATT 
TTGCAGCAGA GTGCAGCTGA CATTGCCAGA GCTGAGCTGT TGCTTGAGGA AGCTAAGAGA 
GCAAGCAAAA GTGCAACAGA TGTTAAAGTC ACTGCAGACA TGGTGAAGGA AGCATTAGAA 
GAAGCAGAAA AGGCCCAGGT TGCAGCAGAG AAGGCGATTA AACAAGCTGA TGAGGATATC 
CAAGGAACCC AAAACCTGCT AACATCGATT GAATCTGAAA CGGCAGCTTC TGAGGAAACC 
CTGACCAACG CCTCCCAGCG CATCAGCAAG CTTGAGAGGA ACGTGGAAGA GCTTAAGCGT 
AAAGCTGCCC AGAACTCTGG GGAGGCAGAA TATATCGAAA AAGTAGTATA TTCTGTAAAA 
CAGAATGCAG ACGATGTTAA GAAGACTCTA GATGGCGAAC TTGATGAAAA GTATAAGAAG 
GTAGAAAGTT TAATTGCCCA AAAAACTGAA GAGTCAGCAG ATGCCAGGAG GAAAGCTGAG 
CTGCTACAAA ATGAAGCAAA AACACTCTTG GCTCAAGCTA ACAGCAAGCT CCAGCTGTTG 
GAAGACTTAG AAAGAAAATA TGAGGACAAT CAAAAATACT TAGAAGATAA AGCTCAAGAA 
TTGGTGCGAC TGGAAGGAGA GGTTCGCTCC CTCCTTAAGG ACATAAGTGA GAAAGTTGCG 
GTTTACAGCA CCTGCTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515055.2
CDS	103..5463
Translation	MGLLQVFAFGVLALWGTRVCAQEPEFSYGCAEGSCYPATGDLLIGRAQKLSVTSTCGLHKPEPYCIVSHLQEDKKCFICDSRDPYHETLNPDSHLIENVVTTFAPNRLKIWWQSENGVENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKAWGVYRYFAYDCESSFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIFRALDPAFKIEDPYSPRIQNLLKITNLRIKFVKLHTLGDNLLDSRMEIREKYYYAVYDMVVRGNCFCYGHASECAPVDGVNEEVEGMVHGHCMCRHNTKGLNCELCMDFYHDLPWRPAEGRNSNACKKCNCNEHSSSCHFDMAVFLATGNVSGGVCDNCQHNTMGRNCEQCKPFYFQHPERDIRDPNLCEPCTCDPAGSENGGICDGYTDFSVGLIAGQCRCKLHVEGERCDVCKEGFYDLSAEDPYGCKSCACNPLGTIPGGNPCDSETGYCYCKRLVTGQRCDQCLPQHWGLSNDLDGCRPCDCDLGGALNNSCSEDSGQCSCLPHMIGRQCNEVESGYYFTTLDHYIYEAEEANLGPGVIVVERQYIQDRIPSWTGPGFVRVPEGAYLEFFIDNIPYSMEYEILIRYEPQLPDHWEKAVITVQRPGKIPASSRCGNTVPDDDNQVVSLSPGSRYVVLPRPVCFEKGMNYTVRLELPQYTASGSDVESPYTFIDSLVLMPYCKSLDIFTVGGSGDGEVTNSAWETFQRYRCLENSRSVVKTPMTDVCRNIIFSISALIHQTGLACECDPQGSLSSVCDPNGGQCQCRPNVVGRTCNRCAPGTFGFGPNGCKPCDCHLQGSASAFCDAITGQCHCFQGIYARQCDRCLPGYWGFPSCQPCQCNGHALDCDTVTGECLSCQDYTTGHNCERCLAGYYGDPIIGSGDHCRPCPCPDGPDSGRQFARSCYQDPVTLQLACVCDPGYIGSRCDDCASGFFGNPSDFGGSCQPCQCHHNIDTTDPEACDKETGRCLKCLYHTEGDHCQLCQYGYYGDALRQDCRKCVCNYLGTVKEHCNGSDCHCDKATGQCSCLPNVIGQNCDRCAPNTWQLASGTGCGPCNCNAAHSFGPSCNEFTGQCQCMPGFGGRTCSECQELFWGDPDVECRACDCDPRGIETPQCDQSTGQCVCVEGVEGPRCDKCTRGYSGVFPDCTPCHQCFALWDAIIGELTNRTHKFLEKAKALKISGVIGPYRETVDSVEKKVNEIKDILAQSPAAEPLKNIGILFEEAEKLTKDVTEKMAQVEVKLTDTASQSNSTAGELGALQAEAESLDKTVKELAEQLEFIKNSDIQGALDSITKYFQMSLEAEKRVNASTTDPNSTVEQSALTRDRVEDLMLERESPFKEQQEEQARLLDELAGKLQSLDLSAVAQMTCGTPPGADCSESECGGPNCRTDEGEKKCGGPGCGGLVTVAHSAWQKAMDFDRDVLSALAEVEQLSKMVSEAKVRADEAKQNAQDVLLKTNATKEKVDKSNEDLRNLIKQIRNFLTEDSADLDSIEAVANEVLKMEMPSTPQQLQNLTEDIRERVETLSQVEVILQQSAADIARAELLLEEAKRASKSATDVKVTADMVKEALEEAEKAQVAAEKAIKQADEDIQGTQNLLTSIESETAASEETLTNASQRISKLERNVEELKRKAAQNSGEAEYIEKVVYSVKQNADDVKKTLDGELDEKYKKVESLIAQKTEESADARRKAELLQNEAKTLLAQANSKLQLLEDLERKYEDNQKYLEDKAQELVRLEGEVRSLLKDISEKVAVYSTCL

Target ORF information:

RefSeq Version	XM_006514992.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus laminin B1 (Lamb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514992.3

ORF Insert Sequence:

ATGGGGCTGC TCCAGGTGTT CGCCTTTGGT GTCCTAGCCC TATGGGGCAC CCGAGTGTGC 
GCTCAGGAAC CGGAGTTCAG CTATGGCTGC GCAGAAGGCA GCTGCTACCC TGCCACTGGC 
GACCTTCTCA TCGGCCGAGC GCAAAAGCTC TCCGTGACTT CGACATGTGG ACTGCACAAA 
CCAGAGCCCT ACTGTATTGT TAGCCACCTG CAGGAGGACA AGAAATGCTT CATATGTGAC 
TCCCGAGACC CTTATCACGA GACCCTCAAC CCCGACAGCC ATCTCATTGA GAACGTGGTC 
ACCACATTTG CTCCAAACCG CCTTAAGATC TGGTGGCAAT CGGAAAATGG TGTGGAGAAC 
GTGACCATCC AACTGGACCT GGAAGCAGAA TTCCATTTCA CTCATCTCAT CATGACCTTC 
AAGACATTCC GCCCAGCCGC CATGCTGATC GAGCGGTCTT CTGACTTTGG GAAGGCTTGG 
GGCGTGTACA GATACTTCGC CTACGACTGT GAGAGCTCGT TCCCAGGCAT TTCAACTGGA 
CCCATGAAGA AAGTGGATGA CATCATCTGT GACTCTCGAT ATTCTGACAT TGAGCCCTCG 
ACAGAAGGAG AGGTAATATT TCGTGCTTTA GATCCTGCTT TCAAAATTGA AGACCCTTAT 
AGTCCAAGGA TACAGAATCT ATTAAAAATC ACCAACTTGA GAATCAAGTT TGTGAAACTG 
CACACCTTGG GGGATAACCT TTTGGACTCC AGAATGGAAA TCCGAGAGAA GTACTATTAC 
GCTGTTTATG ATATGGTGGT TCGAGGGAAC TGCTTCTGCT ATGGCCACGC CAGTGAATGC 
GCCCCTGTGG ATGGAGTCAA TGAAGAAGTG GAAGGAATGG TTCACGGGCA CTGCATGTGC 
AGACACAACA CCAAAGGCCT GAACTGTGAG CTGTGCATGG ATTTCTACCA CGATTTGCCG 
TGGAGACCTG CTGAAGGCCG GAACAGCAAC GCCTGCAAAA AATGTAACTG CAATGAACAT 
TCCAGCTCGT GTCACTTTGA CATGGCAGTC TTCCTGGCTA CTGGCAACGT CAGCGGGGGA 
GTGTGTGATA ACTGTCAGCA CAACACCATG GGGCGCAACT GTGAACAGTG CAAACCGTTC 
TACTTCCAGC ACCCTGAGAG GGACATCCGG GACCCCAATC TCTGTGAACC ATGTACCTGT 
GACCCAGCTG GTTCTGAGAA TGGCGGGATC TGTGATGGGT ACACTGATTT TTCTGTGGGT 
CTCATTGCTG GTCAGTGTCG GTGCAAATTG CACGTGGAGG GAGAGCGCTG TGATGTTTGT 
AAAGAAGGCT TCTACGACTT AAGTGCTGAA GACCCGTATG GTTGTAAATC ATGTGCTTGC 
AATCCTCTGG GAACAATTCC TGGTGGGAAT CCTTGTGATT CTGAGACTGG CTACTGCTAC 
TGTAAGCGCC TGGTGACAGG ACAGCGCTGT GACCAGTGCC TGCCGCAGCA CTGGGGTTTA 
AGCAATGATT TGGATGGGTG TCGACCTTGT GACTGTGACC TTGGAGGGGC GCTGAACAAT 
AGCTGCTCCG AGGACTCCGG CCAGTGCTCC TGCCTGCCCC ACATGATTGG GCGGCAGTGT 
AACGAGGTGG AGTCCGGTTA CTACTTCACC ACCCTGGACC ACTACATCTA CGAAGCCGAG 
GAAGCCAATC TGGGGCCTGG AGTCATTGTG GTGGAAAGGC AGTACATTCA GGACCGCATT 
CCTTCCTGGA CAGGACCTGG GTTCGTCCGG GTGCCTGAAG GGGCTTATTT GGAGTTTTTC 
ATTGACAACA TACCGTATTC CATGGAGTAT GAAATCCTGA TTCGCTATGA GCCACAGCTG 
CCGGACCACT GGGAGAAAGC TGTCATCACT GTACAGCGGC CGGGGAAGAT TCCAGCCAGC 
AGCCGATGTG GTAACACCGT TCCCGATGAT GACAACCAGG TGGTGTCCTT GTCACCGGGC 
TCAAGATACG TTGTCCTCCC TCGCCCCGTG TGCTTTGAGA AGGGAATGAA CTACACGGTG 
AGGTTGGAGC TGCCCCAGTA TACGGCATCG GGCAGTGACG TGGAGAGCCC TTACACGTTC 
ATCGACTCGC TTGTTCTCAT GCCCTACTGT AAATCGCTGG ACATCTTCAC TGTTGGCGGC 
TCAGGCGATG GGGAGGTCAC CAATAGTGCC TGGGAAACCT TCCAGCGCTA CAGGTGTCTG 
GAGAACAGCA GGAGTGTGGT AAAAACACCC ATGACAGATG TCTGCAGAAA CATTATCTTC 
AGCATTTCTG CCTTGATTCA CCAGACGGGC CTTGCTTGTG AATGTGACCC CCAGGGATCT 
CTGAGTTCTG TGTGTGACCC CAATGGTGGC CAGTGCCAGT GCCGTCCTAA TGTGGTTGGA 
AGAACCTGCA ACAGGTGTGC CCCGGGCACC TTTGGCTTTG GCCCCAACGG ATGCAAACCT 
TGTGACTGCC ATCTGCAAGG GTCTGCCAGT GCCTTCTGCG ATGCGATCAC TGGCCAGTGC 
CACTGTTTCC AGGGCATCTA TGCTCGGCAG TGTGACCGAT GTCTCCCTGG GTATTGGGGC 
TTTCCCAGCT GCCAGCCCTG CCAGTGTAAT GGTCATGCTC TAGACTGTGA CACAGTGACA 
GGGGAGTGTC TGAGCTGTCA GGACTACACC ACGGGCCACA ACTGCGAAAG GTGCCTGGCT 
GGCTACTACG GTGATCCCAT CATTGGGTCA GGAGACCACT GTCGCCCTTG CCCTTGTCCT 
GATGGTCCTG ACAGTGGACG ACAGTTTGCC AGGAGCTGTT ATCAAGACCC CGTCACTCTC 
CAGCTTGCGT GTGTTTGTGA TCCTGGGTAC ATTGGCTCCA GATGTGATGA CTGTGCCTCT 
GGATTTTTTG GCAATCCCTC AGACTTTGGG GGTTCATGTC AACCATGTCA GTGCCACCAC 
AACATTGACA CTACCGATCC AGAAGCCTGT GACAAGGAGA CGGGACGATG CCTCAAGTGC 
CTGTACCACA CAGAAGGGGA CCATTGCCAG CTCTGCCAGT ATGGGTACTA CGGCGATGCT 
CTTCGGCAAG ACTGTAGAAA GTGTGTCTGC AATTACCTGG GCACGGTGAA GGAACATTGT 
AATGGCTCTG ACTGCCACTG TGACAAAGCC ACTGGTCAGT GCTCGTGCCT TCCCAATGTG 
ATCGGGCAGA ACTGTGACCG GTGTGCGCCC AACACCTGGC AGCTGGCTAG CGGGACTGGC 
TGCGGGCCCT GCAATTGCAA TGCTGCGCAT TCCTTTGGGC CATCCTGCAA CGAGTTCACA 
GGGCAGTGCC AGTGCATGCC GGGCTTTGGA GGCCGAACCT GCAGCGAGTG CCAGGAGCTC 
TTCTGGGGAG ACCCTGATGT GGAATGCCGA GCCTGTGACT GTGATCCCAG GGGCATTGAG 
ACACCTCAGT GTGACCAGTC CACGGGCCAG TGTGTCTGTG TGGAGGGTGT AGAGGGTCCT 
CGCTGCGACA AGTGCACCAG AGGTTACTCG GGGGTCTTTC CTGACTGCAC ACCCTGCCAC 
CAGTGCTTTG CTCTCTGGGA TGCTATCATT GGTGAGCTGA CCAACAGGAC CCACAAATTC 
CTGGAGAAAG CCAAGGCTCT GAAAATCAGT GGTGTGATTG GTCCCTACCG AGAGACCGTG 
GACTCTGTAG AGAAGAAAGT CAATGAGATA AAAGACATCC TGGCCCAGAG CCCAGCAGCG 
GAACCACTGA AAAACATTGG CATTCTCTTC GAGGAGGCAG AGAAACTAAC CAAAGATGTC 
ACAGAAAAGA TGGCGCAGGT AGAAGTGAAA TTAACTGATA CAGCTTCACA GAGTAACAGC 
ACAGCTGGAG AGCTCGGCGC ACTGCAGGCA GAAGCAGAGA GCCTTGACAA GACCGTGAAG 
GAGCTGGCAG AACAGCTGGA GTTTATCAAA AACTCCGATA TTCAGGGCGC CTTGGATAGC 
ATCACCAAGT ATTTCCAGAT GTCTCTTGAG GCAGAGAAGC GGGTGAATGC CTCCACCACA 
GACCCCAACA GCACTGTGGA GCAGTCTGCC CTCACGCGAG ACAGAGTAGA AGATCTGATG 
TTGGAGCGAG AGTCTCCGTT CAAGGAGCAG CAGGAGGAAC AGGCACGCCT CCTGGACGAA 
CTGGCCGGCA AACTGCAAAG TCTCGACCTG TCGGCTGTTG CACAGATGAC CTGTGGAACA 
CCTCCAGGGG CTGACTGTTC TGAAAGTGAA TGTGGTGGCC CCAACTGCAG AACTGACGAA 
GGAGAGAAGA AGTGTGGGGG GCCTGGCTGT GGTGGTCTGG TCACTGTGGC CCACAGTGCT 
TGGCAGAAAG CCATGGATTT TGACCGTGAT GTCCTGAGTG CCCTGGCTGA AGTCGAACAG 
CTCTCCAAGA TGGTCTCTGA AGCAAAAGTG AGAGCAGATG AGGCGAAGCA GAATGCGCAG 
GATGTCCTGT TAAAAACAAA TGCTACCAAA GAAAAAGTGG ACAAGAGCAA CGAGGACCTG 
CGGAACCTCA TCAAGCAGAT CAGAAACTTC CTGACTGAGG ATAGTGCTGA TCTAGACAGT 
ATTGAAGCAG TTGCTAATGA AGTACTGAAA ATGGAAATGC CTAGCACGCC ACAGCAGTTA 
CAGAACCTAA CAGAAGACAT TCGGGAGCGA GTTGAAACCC TCTCTCAAGT AGAGGTTATT 
TTGCAGCAGA GTGCAGCTGA CATTGCCAGA GCTGAGCTGT TGCTTGAGGA AGCTAAGAGA 
GCAAGCAAAA GTGCAACAGA TGTTAAAGTC ACTGCAGACA TGGTGAAGGA AGCATTAGAA 
GAAGCAGAAA AGGCCCAGGT TGCAGCAGAG AAGGCGATTA AACAAGCTGA TGAGGATATC 
CAAGGAACCC AAAACCTGCT AACATCGATT GAATCTGAAA CGGCAGCTTC TGAGGAAACC 
CTGACCAACG CCTCCCAGCG CATCAGCAAG CTTGAGAGGA ACGTGGAAGA GCTTAAGCGT 
AAAGCTGCCC AGAACTCTGG GGAGGCAGAA TATATCGAAA AAGTAGTATA TTCTGTAAAA 
CAGAATGCAG ACGATGTTAA GAAGACTCTA GATGGCGAAC TTGATGAAAA GTATAAGAAG 
GTAGAAAGTT TAATTGCCCA AAAAACTGAA GAGTCAGCAG ATGCCAGGAG GAAAGCTGAG 
CTGCTACAAA ATGAAGCAAA AACACTCTTG GCTCAAGCTA ACAGCAAGCT CCAGCTGTTG 
GAAGACTTAG AAAGAAAATA TGAGGACAAT CAAAAATACT TAGAAGATAA AGCTCAAGAA 
TTGGTGCGAC TGGAAGGAGA GGTTCGCTCC CTCCTTAAGG ACATAAGTGA GAAAGTTGCG 
GTTTACAGCA CCTGCTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Development (Cambridge, England)144(20)3686-3697(2017 10)
Casoni F,Croci L,Bosone C,D'Ambrosio R,Badaloni A,Gaudesi D,Barili V,Sarna JR,Tessarollo L,Cremona O,Hawkes R,Warming S,Consalez GG

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Lamb1 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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