Reg3a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Reg3a
Entrez Gene ID	19694
Full Name	regenerating islet-derived 3 alpha
Synonyms	AV063448
General protein information	Preferred Names regenerating islet-derived 3 alpha Names regenerating islet-derived protein 3-alpha REG-3-alpha RegIII (alpha) generating islet-derived, mouse homolog 3 alpha islet of Langerhans regenerating protein 3 lithostathine 3 pancreatitis-associated protein 2 reg III-alpha regenerating islet-derived protein III-alpha
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 C3\|6 34.76 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011259.1	NP_035389.1	regenerating islet-derived protein 3-alpha precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Reg3a	NP_035389.1
Rattus norvegicus (Norway rat)	Reg3a	NP_001139318.1

Gene Ontology
Bibliography

Related articles in PubMed

IL-36? Induced by the TLR3-SLUG-VDR Axis Promotes Wound Healing via REG3A.
Jiang Z, Liu Y, Li C, Chang L, Wang W, Wang Z, Gao X, Ryffel B, Wu Y, Lai Y
The Journal of investigative dermatology137(12)2620-2629(2017 Dec)

Recombinant Reg3? protein protects against experimental acute pancreatitis in mice.
Yu LT, Yang MQ, Liu JL, Alfred MO, Li X, Zhang XQ, Zhang J, Wu MY, Wang M, Luo C
Molecular and cellular endocrinology422150-159(2016 Feb)

The Reg3? (HIP/PAP) Lectin Suppresses Extracellular Oxidative Stress in a Murine Model of Acute Liver Failure.
Moniaux N, Darnaud M, Garbin K, Dos Santos A, Guettier C, Samuel D, Amouyal G, Amouyal P, Br?chot C, Faivre J
PloS one10(5)e0125584(2015)

The antimicrobial protein REG3A regulates keratinocyte proliferation and differentiation after skin injury.
Lai Y, Li D, Li C, Muehleisen B, Radek KA, Park HJ, Jiang Z, Li Z, Lei H, Quan Y, Zhang T, Wu Y, Kotol P, Morizane S, Hata TR, Iwatsuki K, Tang C, Gallo RL
Immunity37(1)74-84(2012 Jul)

Regenerating islet-derived 3-alpha is a biomarker of gastrointestinal graft-versus-host disease.
Ferrara JL, Harris AC, Greenson JK, Braun TM, Holler E, Teshima T, Levine JE, Choi SW, Huber E, Landfried K, Akashi K, Vander Lugt M, Reddy P, Chin A, Zhang Q, Hanash S, Paczesny S
Blood118(25)6702-8(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

skin injury increases IL-36gamma via the activation of TLR3-SLUG-VDR axis and IL-36gamma induces REG3A to promote wound healing
Title: IL-36? Induced by the TLR3-SLUG-VDR Axis Promotes Wound Healing via REG3A.

Reg3alpha is an extracellular matrix-targeted reactive oxygen species scavenger that binds the fibrin scaffold resulting from hepatocyte death during acute liver failure.
Title: The Reg3? (HIP/PAP) Lectin Suppresses Extracellular Oxidative Stress in a Murine Model of Acute Liver Failure.

Data show that regenerating islet-derived 3-alpha (REG3alpha) is a plasma biomarker of gastrointestinal tract graft-versus-host disease that can be combined with clinical stage and histologic grade to improve risk stratification of patients.
Title: Regenerating islet-derived 3-alpha is a biomarker of gastrointestinal graft-versus-host disease.

Reg2, Reg3alpha, and Reg3beta were abundantly expressed in most acinar cells of the pancreas with clear age-dependent and isoform-specific patterns.
Title: Coordinated age-dependent and pancreatic-specific expression of mouse Reg2Reg3?, and Reg3? genes.

Reg3alpha stimulates beta-cell replication, by activating Akt kinase and increasing the levels of cyclin D1/CDK4.
Title: Overexpression of Reg3alpha increases cell growth and the levels of cyclin D1 and CDK4 in insulinoma cells.

The following Reg3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Reg3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07761	NM_011259.1 Latest version!	Mus musculus regenerating islet-derived 3 alpha (Reg3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Reg3a ( NM_011259.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035389.1 Mus musculus regenerating islet-derived 3 alpha (Reg3a), mRNA.

Price & Availability↑

CloneID	OMu07761
Clone ID Related Accession (Same CDS sequence)	NM_011259.1
Accession Version	NM_011259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	regenerating islet-derived protein 3-alpha precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D63358.1. ##Evidence-Data-START## Transcript exon combination :: BC119153.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCCTC ACCTGGTCCT CAACAGTATT TCCTGGATGC TGCTCTCCTG CCTGTTGTTT 
GTATTTCAGG TACAAGGTGA AGACTTCCAG AAGGAAGTGC CCTCTCCACG TACCAGCTGC 
CCCATGGGTT ACAAGGCTTA TCGCTCCCAC TGCTATGCCT TAGTTATGAC ACCTAAATCC 
TGGTTTCAAG CAGATCTGGT CTGCCAGAAG AGACCCTCAG GACATCTCGT GTCTATTCTT 
AGTGGAGGTG AGGCTTCCTT TGTGTCCTCC TTGGTGAACG GCAGAGTGGA CAACTACCAA 
GACATCTGGA TTGGGCTCCA TGATCCAACA ATGGGTCAAC AACCCAATGG AGGTGGATGG 
GAGTGGAGTA ACTCCGATGT GCTGAATTAT CTTAACTGGG ATGGGGATCC TTCCTCTACT 
GTCAACCGTG GTCACTGTGG GAGTCTGACA GCAAGTTCAG GGTTTCTGAA GTGGGGAGAC 
TATTACTGCG ATGGGACATT ACCATTTGTC TGCAAGTTCA AGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035389.1
CDS	79..606
Translation	MLPHLVLNSISWMLLSCLLFVFQVQGEDFQKEVPSPRTSCPMGYKAYRSHCYALVMTPKSWFQADLVCQKRPSGHLVSILSGGEASFVSSLVNGRVDNYQDIWIGLHDPTMGQQPNGGGWEWSNSDVLNYLNWDGDPSSTVNRGHCGSLTASSGFLKWGDYYCDGTLPFVCKFKQ

Target ORF information:

RefSeq Version	NM_011259.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus regenerating islet-derived 3 alpha (Reg3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011259.1

ORF Insert Sequence:

ATGCTGCCTC ACCTGGTCCT CAACAGTATT TCCTGGATGC TGCTCTCCTG CCTGTTGTTT 
GTATTTCAGG TACAAGGTGA AGACTTCCAG AAGGAAGTGC CCTCTCCACG TACCAGCTGC 
CCCATGGGTT ACAAGGCTTA TCGCTCCCAC TGCTATGCCT TAGTTATGAC ACCTAAATCC 
TGGTTTCAAG CAGATCTGGT CTGCCAGAAG AGACCCTCAG GACATCTCGT GTCTATTCTT 
AGTGGAGGTG AGGCTTCCTT TGTGTCCTCC TTGGTGAACG GCAGAGTGGA CAACTACCAA 
GACATCTGGA TTGGGCTCCA TGATCCAACA ATGGGTCAAC AACCCAATGG AGGTGGATGG 
GAGTGGAGTA ACTCCGATGT GCTGAATTAT CTTAACTGGG ATGGGGATCC TTCCTCTACT 
GTCAACCGTG GTCACTGTGG GAGTCTGACA GCAAGTTCAG GGTTTCTGAA GTGGGGAGAC 
TATTACTGCG ATGGGACATT ACCATTTGTC TGCAAGTTCA AGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

IL-36? Induced by the TLR3-SLUG-VDR Axis Promotes Wound Healing via REG3A.
The Journal of investigative dermatology137(12)2620-2629(2017 Dec)
Jiang Z,Liu Y,Li C,Chang L,Wang W,Wang Z,Gao X,Ryffel B,Wu Y,Lai Y

Recombinant Reg3? protein protects against experimental acute pancreatitis in mice.
Molecular and cellular endocrinology422150-159(2016 Feb)
Yu LT,Yang MQ,Liu JL,Alfred MO,Li X,Zhang XQ,Zhang J,Wu MY,Wang M,Luo C

The Reg3? (HIP/PAP) Lectin Suppresses Extracellular Oxidative Stress in a Murine Model of Acute Liver Failure.
PloS one10(5)e0125584(2015)
Moniaux N,Darnaud M,Garbin K,Dos Santos A,Guettier C,Samuel D,Amouyal G,Amouyal P,Br?chot C,Faivre J

The antimicrobial protein REG3A regulates keratinocyte proliferation and differentiation after skin injury.
Immunity37(1)74-84(2012 Jul)
Lai Y,Li D,Li C,Muehleisen B,Radek KA,Park HJ,Jiang Z,Li Z,Lei H,Quan Y,Zhang T,Wu Y,Kotol P,Morizane S,Hata TR,Iwatsuki K,Tang C,Gallo RL

Regenerating islet-derived 3-alpha is a biomarker of gastrointestinal graft-versus-host disease.
Blood118(25)6702-8(2011 Dec)
Ferrara JL,Harris AC,Greenson JK,Braun TM,Holler E,Teshima T,Levine JE,Choi SW,Huber E,Landfried K,Akashi K,Vander Lugt M,Reddy P,Chin A,Zhang Q,Hanash S,Paczesny S

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Reg3a cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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