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Pcyt1b cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Pcyt1b
Entrez Gene ID 286936
Full Name phosphate cytidylyltransferase 1, choline, beta
Synonyms CCT-beta,CTB,Cctbeta
General protein information
Preferred Names

phosphate cytidylyltransferase 1, choline, beta

Names

choline-phosphate cytidylyltransferase B
CCT B
CT B
CTP:phosphocholine cytidylyltransferase B
choline phosphate cytidylyltransferase 1 beta
phosphate cytidylyltransferase 1, choline, beta isoform
phosphorylcholine transferase B
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

X

Xq22

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_008773254.2 XP_008771476.1 choline-phosphate cytidylyltransferase B isoform X3
XM_006257016.3 XP_006257078.1 choline-phosphate cytidylyltransferase B isoform X1
XM_006257017.3 XP_006257079.1 choline-phosphate cytidylyltransferase B isoform X2
NM_173151.1 NP_775174.1 choline-phosphate cytidylyltransferase B

Pathways from BioSystems


Homologies


Bos taurus (cattle) PCYT1B NP_001179980.1
Oryza sativa (rice) Os10g0578900 NP_001065503.1
Xenopus tropicalis (tropical clawed frog) pcyt1b XP_004911814.1
Homo sapiens (human) PCYT1B NP_004836.2
Canis lupus familiaris (dog) PCYT1B XP_548900.2
Rattus norvegicus (Norway rat) Pcyt1b NP_775174.1
Danio rerio (zebrafish) pcyt1ba NP_001076415.1
Mus musculus (house mouse) Pcyt1b NP_997593.1
Oryza sativa (rice) Os08g0161800 NP_001061052.1
PCYT1B XP_001090055.2
Gallus gallus (chicken) PCYT1B XP_416793.4

Gene Ontology


Bibliography

Related articles in PubMed

Functions of membrane binding domain of CTP:phosphocholine cytidylyltransferase in alveolar type II cells.
Ridsdale R, Tseu I, Wang J, Post M
American journal of respiratory cell and molecular biology43(1)74-87(2010 Jul)

Crystal structure of a mammalian CTP: phosphocholine cytidylyltransferase catalytic domain reveals novel active site residues within a highly conserved nucleotidyltransferase fold.
Lee J, Johnson J, Ding Z, Paetzel M, Cornell RB
The Journal of biological chemistry284(48)33535-48(2009 Nov)

Phosphatidylcholine biosynthesis via CTP:phosphocholine cytidylyltransferase 2 facilitates neurite outgrowth and branching.
Carter JM, Demizieux L, Campenot RB, Vance DE, Vance JE
The Journal of biological chemistry283(1)202-12(2008 Jan)

Disruption of CCTbeta2 expression leads to gonadal dysfunction.
Jackowski S, Rehg JE, Zhang YM, Wang J, Miller K, Jackson P, Karim MA
Molecular and cellular biology24(11)4720-33(2004 Jun)

Gene structure, expression and identification of a new CTP:phosphocholine cytidylyltransferase beta isoform.
Karim M, Jackson P, Jackowski S
Biochimica et biophysica acta1633(1)1-12(2003 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The 2.2-A structure reveals a CCT236 homodimer in complex with the reaction product, CDP-choline.
Title: Crystal structure of a mammalian CTP: phosphocholine cytidylyltransferase catalytic domain reveals novel active site residues within a highly conserved nucleotidyltransferase fold.

Results highlight the importance of CTbeta2 in neurons for promoting neurite outgrowth and branching and represent the first identification of a lipid biosynthetic enzyme that facilitates these functions.
Title: Phosphatidylcholine biosynthesis via CTP:phosphocholine cytidylyltransferase 2 facilitates neurite outgrowth and branching.

The following Pcyt1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcyt1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa03574 NM_173151.1
Latest version!		 Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $188.30
$269.00
ORa39772 XM_006257016.3
Latest version!		 Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
ORa39773 XM_006257017.3
Latest version!		 Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
ORa44551 XM_008773254.2
Latest version!		 Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa03574
Clone ID Related Accession (Same CDS sequence) NM_173151.1
Accession Version NM_173151.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1110bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-24
Organism Rattus norvegicus(Norway rat)
Product choline-phosphate cytidylyltransferase B
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF190256.1. ##Evidence-Data-START## Transcript exon combination :: AF190256.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## 

1
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361
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601
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721
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961
1021
1081
ATGCCAGTAG TTACCACTGA TGCTGAGTCA GAAACAGGTA TCCCAAAATC CCTTTCCAAT 
GAGCCTCCCT CAGAAACCAT GGAGGAAATA GAGCACACAT GCCCACAGCC TCGACTGACC 
CTGACTGCAC CAGCCCCATT TGCCGATGAG TCCAGCTGCC AGTGCCAAGC GCCTCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACTCCAGTTG ACAGGCCTGT TAGAGTATAT 
GCAGACGGAA TATTCGACCT CTTCCACTCA GGTCATGCAA GGGCACTTAT GCAAGCAAAA 
ACCCTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGACCT AACTCACAAA 
TTCAAAGGTT TTACTGTGAT GAATGAGGCA GAGAGATATG AAGCTCTCAG ACACTGTCGC 
TACGTGGACG AAGTCATCAG AGATGCACCA TGGACTCTTA CTCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCACGATGAC ATTCCGTACT CATCTGCCGG TTCTGATGAC 
GTTTATAAGC ATATCAAGGA AGCAGGCATG TTTGTTCCAA CACAAAGAAC AGAAGGCATC 
TCAACATCAG ACATCATCAC CAGAATTGTC CGTGACTATG ACGTATATGC CCGGCGTAAT 
CTCCAGAGAG GGTACACAGC CAAGGAGCTG AATGTCAGCT TTATAAACGA GAAGAAGTAC 
CGTTTCCAGA ACCAGGTGGA CAAGATGAAA GAAAAGGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TTAACAGAGT AGAAGAAAAG AGTCACGACC TAATTCAAAA GTGGGAAGAA 
AAATCAAGAG AATTCATTGG CAACTTCCTA GAATTGTTTG GGCCAGATGG TGCATGGAAG 
CAGATGTTCC AGGAGAGGAG TAGCCGGATG CTACAGGCCT TATCTCCAAA GCAGAGTCCT 
GTGAGCAGCC CGACCCGGAG CCGGTCCCCT TCCCGCTCTC CATCTCCTAC CTTCTCCTGG 
CTCCCAAACA AGACCTCTCC CCCTTCCTCA CCCAAAGCAG CCTCAGCCTC CATCAGCAGC 
ATGAGTGAGG GGGATGAGGA TGAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_775174.1
CDS11..1120
Translation

Target ORF information:

RefSeq Version NM_173151.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_173151.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCCAGTAG TTACCACTGA TGCTGAGTCA GAAACAGGTA TCCCAAAATC CCTTTCCAAT 
GAGCCTCCCT CAGAAACCAT GGAGGAAATA GAGCACACAT GCCCACAGCC TCGACTGACC 
CTGACTGCAC CAGCCCCATT TGCCGATGAG TCCAGCTGCC AGTGCCAAGC GCCTCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACTCCAGTTG ACAGGCCTGT TAGAGTATAT 
GCAGACGGAA TATTCGACCT CTTCCACTCA GGTCATGCAA GGGCACTTAT GCAAGCAAAA 
ACCCTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGACCT AACTCACAAA 
TTCAAAGGTT TTACTGTGAT GAATGAGGCA GAGAGATATG AAGCTCTCAG ACACTGTCGC 
TACGTGGACG AAGTCATCAG AGATGCACCA TGGACTCTTA CTCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCACGATGAC ATTCCGTACT CATCTGCCGG TTCTGATGAC 
GTTTATAAGC ATATCAAGGA AGCAGGCATG TTTGTTCCAA CACAAAGAAC AGAAGGCATC 
TCAACATCAG ACATCATCAC CAGAATTGTC CGTGACTATG ACGTATATGC CCGGCGTAAT 
CTCCAGAGAG GGTACACAGC CAAGGAGCTG AATGTCAGCT TTATAAACGA GAAGAAGTAC 
CGTTTCCAGA ACCAGGTGGA CAAGATGAAA GAAAAGGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TTAACAGAGT AGAAGAAAAG AGTCACGACC TAATTCAAAA GTGGGAAGAA 
AAATCAAGAG AATTCATTGG CAACTTCCTA GAATTGTTTG GGCCAGATGG TGCATGGAAG 
CAGATGTTCC AGGAGAGGAG TAGCCGGATG CTACAGGCCT TATCTCCAAA GCAGAGTCCT 
GTGAGCAGCC CGACCCGGAG CCGGTCCCCT TCCCGCTCTC CATCTCCTAC CTTCTCCTGG 
CTCCCAAACA AGACCTCTCC CCCTTCCTCA CCCAAAGCAG CCTCAGCCTC CATCAGCAGC 
ATGAGTGAGG GGGATGAGGA TGAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa39772
Clone ID Related Accession (Same CDS sequence) XM_006257016.3
Accession Version XM_006257016.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1050bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product choline-phosphate cytidylyltransferase B isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257016.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGTCGTGCC ACGCTTCTGA ATATGAGGTT CCTTTCAGCA GAGTGTCCCG GGTGTGGACC 
CTGACTGCAC CAGCCCCATT TGCCGATGAG TCCAGCTGCC AGTGCCAAGC GCCTCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACTCCAGTTG ACAGGCCTGT TAGAGTATAT 
GCAGACGGAA TATTCGACCT CTTCCACTCA GGTCATGCAA GGGCACTTAT GCAAGCAAAA 
ACCCTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGACCT AACTCACAAA 
TTCAAAGGTT TTACTGTGAT GAATGAGGCA GAGAGATATG AAGCTCTCAG ACACTGTCGC 
TACGTGGACG AAGTCATCAG AGATGCACCA TGGACTCTTA CTCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCACGATGAC ATTCCGTACT CATCTGCCGG TTCTGATGAC 
GTTTATAAGC ATATCAAGGA AGCAGGCATG TTTGTTCCAA CACAAAGAAC AGAAGGCATC 
TCAACATCAG ACATCATCAC CAGAATTGTC CGTGACTATG ACGTATATGC CCGGCGTAAT 
CTCCAGAGAG GGTACACAGC CAAGGAGCTG AATGTCAGCT TTATAAACGA GAAGAAGTAC 
CGTTTCCAGA ACCAGGTGGA CAAGATGAAA GAAAAGGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TTAACAGAGT AGAAGAAAAG AGTCACGACC TAATTCAAAA GTGGGAAGAA 
AAATCAAGAG AATTCATTGG CAACTTCCTA GAATTGTTTG GGCCAGATGG TGCATGGAAG 
CAGATGTTCC AGGAGAGGAG TAGCCGGATG CTACAGGCCT TATCTCCAAA GCAGAGTCCT 
GTGAGCAGCC CGACCCGGAG CCGGTCCCCT TCCCGCTCTC CATCTCCTAC CTTCTCCTGG 
CTCCCAAACA AGACCTCTCC CCCTTCCTCA CCCAAAGCAG CCTCAGCCTC CATCAGCAGC 
ATGAGTGAGG GGGATGAGGA TGAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006257078.1
CDS201..1250
Translation

Target ORF information:

RefSeq Version XM_006257016.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006257016.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGTCGTGCC ACGCTTCTGA ATATGAGGTT CCTTTCAGCA GAGTGTCCCG GGTGTGGACC 
CTGACTGCAC CAGCCCCATT TGCCGATGAG TCCAGCTGCC AGTGCCAAGC GCCTCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACTCCAGTTG ACAGGCCTGT TAGAGTATAT 
GCAGACGGAA TATTCGACCT CTTCCACTCA GGTCATGCAA GGGCACTTAT GCAAGCAAAA 
ACCCTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGACCT AACTCACAAA 
TTCAAAGGTT TTACTGTGAT GAATGAGGCA GAGAGATATG AAGCTCTCAG ACACTGTCGC 
TACGTGGACG AAGTCATCAG AGATGCACCA TGGACTCTTA CTCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCACGATGAC ATTCCGTACT CATCTGCCGG TTCTGATGAC 
GTTTATAAGC ATATCAAGGA AGCAGGCATG TTTGTTCCAA CACAAAGAAC AGAAGGCATC 
TCAACATCAG ACATCATCAC CAGAATTGTC CGTGACTATG ACGTATATGC CCGGCGTAAT 
CTCCAGAGAG GGTACACAGC CAAGGAGCTG AATGTCAGCT TTATAAACGA GAAGAAGTAC 
CGTTTCCAGA ACCAGGTGGA CAAGATGAAA GAAAAGGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TTAACAGAGT AGAAGAAAAG AGTCACGACC TAATTCAAAA GTGGGAAGAA 
AAATCAAGAG AATTCATTGG CAACTTCCTA GAATTGTTTG GGCCAGATGG TGCATGGAAG 
CAGATGTTCC AGGAGAGGAG TAGCCGGATG CTACAGGCCT TATCTCCAAA GCAGAGTCCT 
GTGAGCAGCC CGACCCGGAG CCGGTCCCCT TCCCGCTCTC CATCTCCTAC CTTCTCCTGG 
CTCCCAAACA AGACCTCTCC CCCTTCCTCA CCCAAAGCAG CCTCAGCCTC CATCAGCAGC 
ATGAGTGAGG GGGATGAGGA TGAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa39773
Clone ID Related Accession (Same CDS sequence) XM_006257017.3
Accession Version XM_006257017.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1050bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product choline-phosphate cytidylyltransferase B isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257017.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAACCTAG TGGTACACCA GAAATGCACC ATGGACAAAG ACAAATTCTC ACGCAAGACC 
CTGACTGCAC CAGCCCCATT TGCCGATGAG TCCAGCTGCC AGTGCCAAGC GCCTCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACTCCAGTTG ACAGGCCTGT TAGAGTATAT 
GCAGACGGAA TATTCGACCT CTTCCACTCA GGTCATGCAA GGGCACTTAT GCAAGCAAAA 
ACCCTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGACCT AACTCACAAA 
TTCAAAGGTT TTACTGTGAT GAATGAGGCA GAGAGATATG AAGCTCTCAG ACACTGTCGC 
TACGTGGACG AAGTCATCAG AGATGCACCA TGGACTCTTA CTCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCACGATGAC ATTCCGTACT CATCTGCCGG TTCTGATGAC 
GTTTATAAGC ATATCAAGGA AGCAGGCATG TTTGTTCCAA CACAAAGAAC AGAAGGCATC 
TCAACATCAG ACATCATCAC CAGAATTGTC CGTGACTATG ACGTATATGC CCGGCGTAAT 
CTCCAGAGAG GGTACACAGC CAAGGAGCTG AATGTCAGCT TTATAAACGA GAAGAAGTAC 
CGTTTCCAGA ACCAGGTGGA CAAGATGAAA GAAAAGGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TTAACAGAGT AGAAGAAAAG AGTCACGACC TAATTCAAAA GTGGGAAGAA 
AAATCAAGAG AATTCATTGG CAACTTCCTA GAATTGTTTG GGCCAGATGG TGCATGGAAG 
CAGATGTTCC AGGAGAGGAG TAGCCGGATG CTACAGGCCT TATCTCCAAA GCAGAGTCCT 
GTGAGCAGCC CGACCCGGAG CCGGTCCCCT TCCCGCTCTC CATCTCCTAC CTTCTCCTGG 
CTCCCAAACA AGACCTCTCC CCCTTCCTCA CCCAAAGCAG CCTCAGCCTC CATCAGCAGC 
ATGAGTGAGG GGGATGAGGA TGAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006257079.1
CDS365..1414
Translation

Target ORF information:

RefSeq Version XM_006257017.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006257017.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAACCTAG TGGTACACCA GAAATGCACC ATGGACAAAG ACAAATTCTC ACGCAAGACC 
CTGACTGCAC CAGCCCCATT TGCCGATGAG TCCAGCTGCC AGTGCCAAGC GCCTCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACTCCAGTTG ACAGGCCTGT TAGAGTATAT 
GCAGACGGAA TATTCGACCT CTTCCACTCA GGTCATGCAA GGGCACTTAT GCAAGCAAAA 
ACCCTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGACCT AACTCACAAA 
TTCAAAGGTT TTACTGTGAT GAATGAGGCA GAGAGATATG AAGCTCTCAG ACACTGTCGC 
TACGTGGACG AAGTCATCAG AGATGCACCA TGGACTCTTA CTCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCACGATGAC ATTCCGTACT CATCTGCCGG TTCTGATGAC 
GTTTATAAGC ATATCAAGGA AGCAGGCATG TTTGTTCCAA CACAAAGAAC AGAAGGCATC 
TCAACATCAG ACATCATCAC CAGAATTGTC CGTGACTATG ACGTATATGC CCGGCGTAAT 
CTCCAGAGAG GGTACACAGC CAAGGAGCTG AATGTCAGCT TTATAAACGA GAAGAAGTAC 
CGTTTCCAGA ACCAGGTGGA CAAGATGAAA GAAAAGGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TTAACAGAGT AGAAGAAAAG AGTCACGACC TAATTCAAAA GTGGGAAGAA 
AAATCAAGAG AATTCATTGG CAACTTCCTA GAATTGTTTG GGCCAGATGG TGCATGGAAG 
CAGATGTTCC AGGAGAGGAG TAGCCGGATG CTACAGGCCT TATCTCCAAA GCAGAGTCCT 
GTGAGCAGCC CGACCCGGAG CCGGTCCCCT TCCCGCTCTC CATCTCCTAC CTTCTCCTGG 
CTCCCAAACA AGACCTCTCC CCCTTCCTCA CCCAAAGCAG CCTCAGCCTC CATCAGCAGC 
ATGAGTGAGG GGGATGAGGA TGAGAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa44551
Clone ID Related Accession (Same CDS sequence) XM_008773254.2
Accession Version XM_008773254.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 996bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product choline-phosphate cytidylyltransferase B isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008773254.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGACCCTGA CTGCACCAGC CCCATTTGCC GATGAGTCCA GCTGCCAGTG CCAAGCGCCT 
CATGAAAAAC TGACCATTGC TCAGGCCCGC TTAGGAACTC CAGTTGACAG GCCTGTTAGA 
GTATATGCAG ACGGAATATT CGACCTCTTC CACTCAGGTC ATGCAAGGGC ACTTATGCAA 
GCAAAAACCC TGTTTCCCAA CAGCTACTTG TTGGTAGGAG TTTGCAGTGA TGACCTAACT 
CACAAATTCA AAGGTTTTAC TGTGATGAAT GAGGCAGAGA GATATGAAGC TCTCAGACAC 
TGTCGCTACG TGGACGAAGT CATCAGAGAT GCACCATGGA CTCTTACTCC AGAGTTTCTG 
GAAAAACACA AGATTGACTT TGTGGCTCAC GATGACATTC CGTACTCATC TGCCGGTTCT 
GATGACGTTT ATAAGCATAT CAAGGAAGCA GGCATGTTTG TTCCAACACA AAGAACAGAA 
GGCATCTCAA CATCAGACAT CATCACCAGA ATTGTCCGTG ACTATGACGT ATATGCCCGG 
CGTAATCTCC AGAGAGGGTA CACAGCCAAG GAGCTGAATG TCAGCTTTAT AAACGAGAAG 
AAGTACCGTT TCCAGAACCA GGTGGACAAG ATGAAAGAAA AGGTCAAGAA TGTGGAGGAA 
AGATCAAAGG AATTTGTTAA CAGAGTAGAA GAAAAGAGTC ACGACCTAAT TCAAAAGTGG 
GAAGAAAAAT CAAGAGAATT CATTGGCAAC TTCCTAGAAT TGTTTGGGCC AGATGGTGCA 
TGGAAGCAGA TGTTCCAGGA GAGGAGTAGC CGGATGCTAC AGGCCTTATC TCCAAAGCAG 
AGTCCTGTGA GCAGCCCGAC CCGGAGCCGG TCCCCTTCCC GCTCTCCATC TCCTACCTTC 
TCCTGGCTCC CAAACAAGAC CTCTCCCCCT TCCTCACCCA AAGCAGCCTC AGCCTCCATC 
AGCAGCATGA GTGAGGGGGA TGAGGATGAG AAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_008771476.1
CDS339..1334
Translation

Target ORF information:

RefSeq Version XM_008773254.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus phosphate cytidylyltransferase 1, choline, beta (Pcyt1b), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_008773254.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGACCCTGA CTGCACCAGC CCCATTTGCC GATGAGTCCA GCTGCCAGTG CCAAGCGCCT 
CATGAAAAAC TGACCATTGC TCAGGCCCGC TTAGGAACTC CAGTTGACAG GCCTGTTAGA 
GTATATGCAG ACGGAATATT CGACCTCTTC CACTCAGGTC ATGCAAGGGC ACTTATGCAA 
GCAAAAACCC TGTTTCCCAA CAGCTACTTG TTGGTAGGAG TTTGCAGTGA TGACCTAACT 
CACAAATTCA AAGGTTTTAC TGTGATGAAT GAGGCAGAGA GATATGAAGC TCTCAGACAC 
TGTCGCTACG TGGACGAAGT CATCAGAGAT GCACCATGGA CTCTTACTCC AGAGTTTCTG 
GAAAAACACA AGATTGACTT TGTGGCTCAC GATGACATTC CGTACTCATC TGCCGGTTCT 
GATGACGTTT ATAAGCATAT CAAGGAAGCA GGCATGTTTG TTCCAACACA AAGAACAGAA 
GGCATCTCAA CATCAGACAT CATCACCAGA ATTGTCCGTG ACTATGACGT ATATGCCCGG 
CGTAATCTCC AGAGAGGGTA CACAGCCAAG GAGCTGAATG TCAGCTTTAT AAACGAGAAG 
AAGTACCGTT TCCAGAACCA GGTGGACAAG ATGAAAGAAA AGGTCAAGAA TGTGGAGGAA 
AGATCAAAGG AATTTGTTAA CAGAGTAGAA GAAAAGAGTC ACGACCTAAT TCAAAAGTGG 
GAAGAAAAAT CAAGAGAATT CATTGGCAAC TTCCTAGAAT TGTTTGGGCC AGATGGTGCA 
TGGAAGCAGA TGTTCCAGGA GAGGAGTAGC CGGATGCTAC AGGCCTTATC TCCAAAGCAG 
AGTCCTGTGA GCAGCCCGAC CCGGAGCCGG TCCCCTTCCC GCTCTCCATC TCCTACCTTC 
TCCTGGCTCC CAAACAAGAC CTCTCCCCCT TCCTCACCCA AAGCAGCCTC AGCCTCCATC 
AGCAGCATGA GTGAGGGGGA TGAGGATGAG AAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Functions of membrane binding domain of CTP:phosphocholine cytidylyltransferase in alveolar type II cells.
American journal of respiratory cell and molecular biology43(1)74-87(2010 Jul)
Ridsdale R,Tseu I,Wang J,Post M


book

Crystal structure of a mammalian CTP: phosphocholine cytidylyltransferase catalytic domain reveals novel active site residues within a highly conserved nucleotidyltransferase fold.
The Journal of biological chemistry284(48)33535-48(2009 Nov)
Lee J,Johnson J,Ding Z,Paetzel M,Cornell RB


book

Phosphatidylcholine biosynthesis via CTP:phosphocholine cytidylyltransferase 2 facilitates neurite outgrowth and branching.
The Journal of biological chemistry283(1)202-12(2008 Jan)
Carter JM,Demizieux L,Campenot RB,Vance DE,Vance JE


book

Disruption of CCTbeta2 expression leads to gonadal dysfunction.
Molecular and cellular biology24(11)4720-33(2004 Jun)
Jackowski S,Rehg JE,Zhang YM,Wang J,Miller K,Jackson P,Karim MA


book

Gene structure, expression and identification of a new CTP:phosphocholine cytidylyltransferase beta isoform.
Biochimica et biophysica acta1633(1)1-12(2003 Jul)
Karim M,Jackson P,Jackowski S