blm cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	blm
Entrez Gene ID	100124311
Full Name	Bloom syndrome RecQ like helicase
Synonyms	recq2,recql2,recql3,xBLM
General protein information	Preferred Names Bloom syndrome RecQ like helicase Names Bloom syndrome protein Bloom syndrome, RecQ helicase-like
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001256908.1	NP_001243837.1	Bloom syndrome protein
XM_012958409.2	XP_012813863.1	Bloom syndrome protein isoform X1
XM_012958409.3	XP_012813863.2	Bloom syndrome protein isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BLM	XP_510594.2
Canis lupus familiaris (dog)	BLM	XP_003434427.1
Mus musculus (house mouse)	Blm	NP_031576.4
	BLM	XP_001097543.1
Gallus gallus (chicken)	BLM	NP_001007088.2
Xenopus tropicalis (tropical clawed frog)	blm	NP_001243837.1
Homo sapiens (human)	BLM	NP_000048.1
Bos taurus (cattle)	BLM	XP_002696629.1
Rattus norvegicus (Norway rat)	Blm	XP_006229520.1
Danio rerio (zebrafish)	blm	XP_701357.4

Gene Ontology

The following blm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the blm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa53160	XM_012958409.3 Latest version!	Xenopus tropicalis Bloom syndrome RecQ like helicase (blm), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa00753	NM_001256908.1 Latest version!	Xenopus tropicalis Bloom syndrome RecQ like helicase (blm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa30300	XM_012958409.2 Latest version!	Xenopus tropicalis Bloom syndrome, RecQ helicase-like (blm), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

blm ( XM_012958409.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813863.2 Xenopus tropicalis Bloom syndrome RecQ like helicase (blm), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa53160
Clone ID Related Accession (Same CDS sequence)	XM_012958409.3
Accession Version	XM_012958409.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Bloom syndrome protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958409.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTC TTCCACAAAA TAATCTTCAG AAGCAGCTAG AACTTTTCTC TGCAAAGGGA 
ACTGCCAACA AGCTTTCTCT TCAGAAACCA AAATCCAGTG TTTTTACCTT TAAAAAGAAA 
TGTTCTCCAA ATGGTTCATT GTCAACTGGC TTCGTTGAAG TTAAGCAAAA TGTTGCTTTA 
AAGGACAAAA ATGTGAATGT GAAACAAAAT GATGTCCAAA CAAACCTCAC AGTTTTGCCA 
AAATCTGCAG AACGCAATAA AATAAATGGA TTTTTCACCC CGGTCTACAC AAAGTCCACA 
CAGCTGCCAC AGACAGTGGC AGTGAAGGAC CACGTCCATA TCAATGACTC TGCAAACAAG 
CACCCAAGAG TGGAGGATTC TGCATCCAAG AAAACAGGGA TTAATACCTC CTTTGGTTCT 
GTTACCAGTT TGGAGGAATG GGATGATCTC GATGATTTTG ACACATCTGT GTCTCCCCCC 
AAGAGTCAGG CTGGCAAAAT AAAAATAACT CCTCAAAATT GCAAGAACAC ATCTCCTGTT 
TCTTCTAAAA TTCAGTGTCT GAGCCCTAAA GGGCCAACCA TTGAAAAGCA GATGTGTGCC 
AAGAAACCAT ATGAGAATAA TGAGATCGCT TCTGAGCCAC AAAAACTTGT GCATGCTCAA 
ACAGCAGAAA TGCCAGACAA ATCATTAGTT TGTTTGGGGT CTGTGGAACA TACAAATTTA 
GGCAGTGCAG AGAAAGACAC ATGTAGGAAC ATTGATTATT CAGGAACTGA TGATATAGAA 
CATGATCAAG AGACGCTTTC ACAGGCACTT ATTGAAAATG AAGATGATTG TGAACCTGAT 
TTTATTCCCC CATCCCCATC AGATGAAAGT CTGTCCTCTC CTCCGGTTTT AAGAGTAATT 
GGTGCACAAA GGAAACACAA AGTCTCTTCT GTAACAGATG TAAATGACTG TGAAAATACA 
GCTGATCACC TGCAGAGCCA ATGTGTCTCT TCTTCACTTG ATTCCAAAGT GCCTTCTCAT 
CTTTTGAGTG TGATGCTTGA AATCTGTGAC TTGGTTGACA AGATTCCAAT ATCAGAGCTA 
CACGTGCTGT CTTGTGGGAT GGACCTCAAG AAAAAAAGGG ACATGCGGAA GCGCCTCTTG 
TTAAATAACT CTGTGTTAAG ATCATCTCCT GCTGACAGCT CAACAGTATC TTTGACTTCC 
TGTCCATCCC AAAATATGGA ATTTGATGTT AGTGCTCCCA AGGATGCAGA AAGTTTCTCT 
GGATCTTCTG TGAACAAAAT CTTTAAATTC AATAAATTGG TTGCAGACGA GTTTGGCACA 
AAGGGAAATG ATAGGGCTTC TGATTTTGCT CCCAATTTCA GGGAGAAAAT GGAGAATAAA 
ACCTCCTTCT CTCTCCAGGC TGGAGGAGAT TCAGTGATGG AAGACTCCTT CAGTTTTCAG 
AGCAATGTTC TTAGCACCAG CCAGTTTAAT ACCCCACAAA ATGAGAAGCC AGTCTCATCT 
AGCACCTGCG CCAGACCCAT CAGTCAGCCT ATAGATGACA TGGATAACCC TGATCTTGAC 
TTTGATATTG ATAACTTTGA TATTGAGGAC TTTGATGATC AGCTCATGGA CAGCCCTGCA 
GCTCCTCCTG TTTCCAGCAA AAATGCTCCA CAGTACCCAA CAATATGTGA GACACAGTTT 
GATTCTGGAA GTAAAGAGAA AGTTACACGT CATAATGTCG GTGACAAAAC TAACACAGAG 
TTATCAGACT CCCTACTAAA GCCACAGATA GTGAACCCAG CCCTTGAACG ATTCAAAAGC 
TTTAACTTTC CTCACTCCAA GGAAATGATG AAGATTTTTC ACAAGAAATT TGGTTTGCAT 
CGGTTCCGGA CGAACCAGCT AGAAGCAATT AATGCATGCT TGCTTGGGGA AGACTGCTTC 
ATTCTTATGC CAACAGGTGG TGGGAAAAGT CTATGCTACC AACTGCCAGG CTGTGTATCC 
CCAGGAGTCA CAATTGTCAT CTCTCCCCTC CGGTCCCTGA TTGTAGATCA GGTTCAAAAG 
CTCACCTCTT TGGATATTCC AGCTACATAT CTCACTGGAG ATAAGACAGA TGCTGAAGCT 
GCTAGTATTT ATTTGCAACT CTCTAAGAAA GACCCTATCA TTAAACTTCT CTATGTCACT 
CCAGAAAAGG TCTGTGCAAG TACCAGGCTA ATATCAACCA TGGAAAATCT ATATGAGCGT 
CAGTTGCTGG CACGATTTGT CATCGATGAG GCTCACTGTG TCAGTCAGTG GGGCCATGAT 
TTCCGGCCAG ACTACAAGCG TCTGAACATG CTACGCCAGA AGTTTCAGTC TGTACCAATG 
ATGGCTCTCA CAGCAACTGC AAACCCCCGT GTACAGAAGG ATATTCTTAA TCAACTAAAG 
ATGACCAAAC CTCAAATATT TACAATGAGC TTTAACCGAG ACAACCTAAA ATATGACGTA 
CTTCCAAAAA AACCCAAGAG GGTGGCATTG GACTGTGTGG AGTGGATCAA GAAATATCAC 
CCAAATGATT CCGGAATCAT TTATTGTCTG TCACGACATG AGTGTGATAC AATGGCAGAT 
ACCCTGCAGA AGGGGGGCCT TGCTGCACTT GCATATCATG CAGGACTAGC TGACTCTAAC 
AGAGATTATG TACAGCATAA GTGGATAAAC CAAGATGATT GCCAGGTAAT CTGTGCTACC 
ATTGCCTTTG GAATGGGCAT TGATAAGCCA GATGTGAGGT ATGTCATTCA TGCTTCTCTA 
CCAAAGTCTG TGGAGGGATA TTACCAGGAG TCAGGGAGAG CTGGCCGAGA CGGGGAAATA 
TCCCACTGCC TCCTGTTCTA CAGCTACAGT GATGTAACAA GAATTCGTAG GCTAATACAA 
ATGGAGAAAG ATGGAAACAG CCATACAAAA CAGACACATT TTAACAACCT ATACAGTATG 
GTCCACTACT GTGAAAATGT AGTGGAGTGT CGCAGGATGC AACTCCTGGC TTATTTTGGT 
GAAAATAATT TTAATCCAAA CTTCTGCAAA GAGAACACAC GAGTCAGTTG TGATAACTGT 
CTTGGGAGGA AGGAGTTCAA ATCCAGAAAT GTGACCGATG ATGTGAGAAA CATTGTGAGA 
TTTGTGCAAG AAAATTGCTC TTCAGCACGT GGCAAGGGTA GAAGCAACAG CAGTCGACTG 
ACCCTTAACA TGATGGTAGA TATATTCCTA GGTTCTAAGT CTGCAAAAAT CCAAACTGGG 
CTATTTGGGA AAGGAGCTGC TTATTCACGG CACAATGCAG AACGTCTCTT CCGGAAGTTA 
GTCCTGGACA GGATATTAGA TGAAGAGCTT TACATTACTG CCAATGATCA GGCTGTGGCC 
TATGTAAAAA CAGGAGAGCG GGCGCAGGCT GTGCTAAATG GATTCTTGAC GGTTGAGTTC 
CAAGACACCG AAAATGCCTC CAGCCTCAGA AAGCAAAAGG CATCAGTTGT CGCTAATACG 
TCCCAAAGGG AAGAAATGGT CAAGAAGTGC CAGGCAGAGC TCACAGAACT CTGTAAACGC 
CTGGGCAAAA TATTTGGAGT TCATTACTTC AACATCTTCA ACACGGCCAC AATACGGAGA 
ATTGCAGAAT CACTATCTCC TGAGCCTGAT GTGTTACTTC AGATTGATGG TGTTACAGAG 
GATAAGTTGG AGAAATATGG TGCAGAGCTA ATTGATGTTT TACAGAAATA TTCTGAGTGG 
ACACTTCCAG TGGAAGATAT TCACCAAAAA TCTAGTGGGC CTGCCAATTT CTCTGCAAGG 
AGATATGATA GTGATGATGA TGATGATGAT GATGGTGAGA GAGGTGAAAA GTCGTCTTAC 
TTCAGCACCA AGAGTAATAA AGGTACAAAG CGGAAGAATG CCCCCACCTT TAGGAAGTCC 
AAAAAACGAA AGACTGGAGG AGATGGTCAG CAGTCCCATC CCAAGAATGC GACAGGTTCA 
TTTTCTAGGA ATAATTCTAC TGCCAAAACA TCTAGTAGCT ACTACAGTGG TTCAAAGACA 
GGAGCTGATA AAAGACCTGG ATTTATGGCA CCACCATTGC CTCAGCCCAA CAGACGCTTC 
CTTAAACCAT CCTACACTCT GTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813863.2
CDS	438..4544
Translation	MAALPQNNLQKQLELFSAKGTANKLSLQKPKSSVFTFKKKCSPNGSLSTGFVEVKQNVALKDKNVNVKQNDVQTNLTVLPKSAERNKINGFFTPVYTKSTQLPQTVAVKDHVHINDSANKHPRVEDSASKKTGINTSFGSVTSLEEWDDLDDFDTSVSPPKSQAGKIKITPQNCKNTSPVSSKIQCLSPKGPTIEKQMCAKKPYENNEIASEPQKLVHAQTAEMPDKSLVCLGSVEHTNLGSAEKDTCRNIDYSGTDDIEHDQETLSQALIENEDDCEPDFIPPSPSDESLSSPPVLRVIGAQRKHKVSSVTDVNDCENTADHLQSQCVSSSLDSKVPSHLLSVMLEICDLVDKIPISELHVLSCGMDLKKKRDMRKRLLLNNSVLRSSPADSSTVSLTSCPSQNMEFDVSAPKDAESFSGSSVNKIFKFNKLVADEFGTKGNDRASDFAPNFREKMENKTSFSLQAGGDSVMEDSFSFQSNVLSTSQFNTPQNEKPVSSSTCARPISQPIDDMDNPDLDFDIDNFDIEDFDDQLMDSPAAPPVSSKNAPQYPTICETQFDSGSKEKVTRHNVGDKTNTELSDSLLKPQIVNPALERFKSFNFPHSKEMMKIFHKKFGLHRFRTNQLEAINACLLGEDCFILMPTGGGKSLCYQLPGCVSPGVTIVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDAEAASIYLQLSKKDPIIKLLYVTPEKVCASTRLISTMENLYERQLLARFVIDEAHCVSQWGHDFRPDYKRLNMLRQKFQSVPMMALTATANPRVQKDILNQLKMTKPQIFTMSFNRDNLKYDVLPKKPKRVALDCVEWIKKYHPNDSGIIYCLSRHECDTMADTLQKGGLAALAYHAGLADSNRDYVQHKWINQDDCQVICATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYSYSDVTRIRRLIQMEKDGNSHTKQTHFNNLYSMVHYCENVVECRRMQLLAYFGENNFNPNFCKENTRVSCDNCLGRKEFKSRNVTDDVRNIVRFVQENCSSARGKGRSNSSRLTLNMMVDIFLGSKSAKIQTGLFGKGAAYSRHNAERLFRKLVLDRILDEELYITANDQAVAYVKTGERAQAVLNGFLTVEFQDTENASSLRKQKASVVANTSQREEMVKKCQAELTELCKRLGKIFGVHYFNIFNTATIRRIAESLSPEPDVLLQIDGVTEDKLEKYGAELIDVLQKYSEWTLPVEDIHQKSSGPANFSARRYDSDDDDDDDGERGEKSSYFSTKSNKGTKRKNAPTFRKSKKRKTGGDGQQSHPKNATGSFSRNNSTAKTSSSYYSGSKTGADKRPGFMAPPLPQPNRRFLKPSYTLF

Target ORF information:

RefSeq Version	XM_012958409.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Bloom syndrome RecQ like helicase (blm), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958409.3

ORF Insert Sequence:

ATGGCTGCTC TTCCACAAAA TAATCTTCAG AAGCAGCTAG AACTTTTCTC TGCAAAGGGA 
ACTGCCAACA AGCTTTCTCT TCAGAAACCA AAATCCAGTG TTTTTACCTT TAAAAAGAAA 
TGTTCTCCAA ATGGTTCATT GTCAACTGGC TTCGTTGAAG TTAAGCAAAA TGTTGCTTTA 
AAGGACAAAA ATGTGAATGT GAAACAAAAT GATGTCCAAA CAAACCTCAC AGTTTTGCCA 
AAATCTGCAG AACGCAATAA AATAAATGGA TTTTTCACCC CGGTCTACAC AAAGTCCACA 
CAGCTGCCAC AGACAGTGGC AGTGAAGGAC CACGTCCATA TCAATGACTC TGCAAACAAG 
CACCCAAGAG TGGAGGATTC TGCATCCAAG AAAACAGGGA TTAATACCTC CTTTGGTTCT 
GTTACCAGTT TGGAGGAATG GGATGATCTC GATGATTTTG ACACATCTGT GTCTCCCCCC 
AAGAGTCAGG CTGGCAAAAT AAAAATAACT CCTCAAAATT GCAAGAACAC ATCTCCTGTT 
TCTTCTAAAA TTCAGTGTCT GAGCCCTAAA GGGCCAACCA TTGAAAAGCA GATGTGTGCC 
AAGAAACCAT ATGAGAATAA TGAGATCGCT TCTGAGCCAC AAAAACTTGT GCATGCTCAA 
ACAGCAGAAA TGCCAGACAA ATCATTAGTT TGTTTGGGGT CTGTGGAACA TACAAATTTA 
GGCAGTGCAG AGAAAGACAC ATGTAGGAAC ATTGATTATT CAGGAACTGA TGATATAGAA 
CATGATCAAG AGACGCTTTC ACAGGCACTT ATTGAAAATG AAGATGATTG TGAACCTGAT 
TTTATTCCCC CATCCCCATC AGATGAAAGT CTGTCCTCTC CTCCGGTTTT AAGAGTAATT 
GGTGCACAAA GGAAACACAA AGTCTCTTCT GTAACAGATG TAAATGACTG TGAAAATACA 
GCTGATCACC TGCAGAGCCA ATGTGTCTCT TCTTCACTTG ATTCCAAAGT GCCTTCTCAT 
CTTTTGAGTG TGATGCTTGA AATCTGTGAC TTGGTTGACA AGATTCCAAT ATCAGAGCTA 
CACGTGCTGT CTTGTGGGAT GGACCTCAAG AAAAAAAGGG ACATGCGGAA GCGCCTCTTG 
TTAAATAACT CTGTGTTAAG ATCATCTCCT GCTGACAGCT CAACAGTATC TTTGACTTCC 
TGTCCATCCC AAAATATGGA ATTTGATGTT AGTGCTCCCA AGGATGCAGA AAGTTTCTCT 
GGATCTTCTG TGAACAAAAT CTTTAAATTC AATAAATTGG TTGCAGACGA GTTTGGCACA 
AAGGGAAATG ATAGGGCTTC TGATTTTGCT CCCAATTTCA GGGAGAAAAT GGAGAATAAA 
ACCTCCTTCT CTCTCCAGGC TGGAGGAGAT TCAGTGATGG AAGACTCCTT CAGTTTTCAG 
AGCAATGTTC TTAGCACCAG CCAGTTTAAT ACCCCACAAA ATGAGAAGCC AGTCTCATCT 
AGCACCTGCG CCAGACCCAT CAGTCAGCCT ATAGATGACA TGGATAACCC TGATCTTGAC 
TTTGATATTG ATAACTTTGA TATTGAGGAC TTTGATGATC AGCTCATGGA CAGCCCTGCA 
GCTCCTCCTG TTTCCAGCAA AAATGCTCCA CAGTACCCAA CAATATGTGA GACACAGTTT 
GATTCTGGAA GTAAAGAGAA AGTTACACGT CATAATGTCG GTGACAAAAC TAACACAGAG 
TTATCAGACT CCCTACTAAA GCCACAGATA GTGAACCCAG CCCTTGAACG ATTCAAAAGC 
TTTAACTTTC CTCACTCCAA GGAAATGATG AAGATTTTTC ACAAGAAATT TGGTTTGCAT 
CGGTTCCGGA CGAACCAGCT AGAAGCAATT AATGCATGCT TGCTTGGGGA AGACTGCTTC 
ATTCTTATGC CAACAGGTGG TGGGAAAAGT CTATGCTACC AACTGCCAGG CTGTGTATCC 
CCAGGAGTCA CAATTGTCAT CTCTCCCCTC CGGTCCCTGA TTGTAGATCA GGTTCAAAAG 
CTCACCTCTT TGGATATTCC AGCTACATAT CTCACTGGAG ATAAGACAGA TGCTGAAGCT 
GCTAGTATTT ATTTGCAACT CTCTAAGAAA GACCCTATCA TTAAACTTCT CTATGTCACT 
CCAGAAAAGG TCTGTGCAAG TACCAGGCTA ATATCAACCA TGGAAAATCT ATATGAGCGT 
CAGTTGCTGG CACGATTTGT CATCGATGAG GCTCACTGTG TCAGTCAGTG GGGCCATGAT 
TTCCGGCCAG ACTACAAGCG TCTGAACATG CTACGCCAGA AGTTTCAGTC TGTACCAATG 
ATGGCTCTCA CAGCAACTGC AAACCCCCGT GTACAGAAGG ATATTCTTAA TCAACTAAAG 
ATGACCAAAC CTCAAATATT TACAATGAGC TTTAACCGAG ACAACCTAAA ATATGACGTA 
CTTCCAAAAA AACCCAAGAG GGTGGCATTG GACTGTGTGG AGTGGATCAA GAAATATCAC 
CCAAATGATT CCGGAATCAT TTATTGTCTG TCACGACATG AGTGTGATAC AATGGCAGAT 
ACCCTGCAGA AGGGGGGCCT TGCTGCACTT GCATATCATG CAGGACTAGC TGACTCTAAC 
AGAGATTATG TACAGCATAA GTGGATAAAC CAAGATGATT GCCAGGTAAT CTGTGCTACC 
ATTGCCTTTG GAATGGGCAT TGATAAGCCA GATGTGAGGT ATGTCATTCA TGCTTCTCTA 
CCAAAGTCTG TGGAGGGATA TTACCAGGAG TCAGGGAGAG CTGGCCGAGA CGGGGAAATA 
TCCCACTGCC TCCTGTTCTA CAGCTACAGT GATGTAACAA GAATTCGTAG GCTAATACAA 
ATGGAGAAAG ATGGAAACAG CCATACAAAA CAGACACATT TTAACAACCT ATACAGTATG 
GTCCACTACT GTGAAAATGT AGTGGAGTGT CGCAGGATGC AACTCCTGGC TTATTTTGGT 
GAAAATAATT TTAATCCAAA CTTCTGCAAA GAGAACACAC GAGTCAGTTG TGATAACTGT 
CTTGGGAGGA AGGAGTTCAA ATCCAGAAAT GTGACCGATG ATGTGAGAAA CATTGTGAGA 
TTTGTGCAAG AAAATTGCTC TTCAGCACGT GGCAAGGGTA GAAGCAACAG CAGTCGACTG 
ACCCTTAACA TGATGGTAGA TATATTCCTA GGTTCTAAGT CTGCAAAAAT CCAAACTGGG 
CTATTTGGGA AAGGAGCTGC TTATTCACGG CACAATGCAG AACGTCTCTT CCGGAAGTTA 
GTCCTGGACA GGATATTAGA TGAAGAGCTT TACATTACTG CCAATGATCA GGCTGTGGCC 
TATGTAAAAA CAGGAGAGCG GGCGCAGGCT GTGCTAAATG GATTCTTGAC GGTTGAGTTC 
CAAGACACCG AAAATGCCTC CAGCCTCAGA AAGCAAAAGG CATCAGTTGT CGCTAATACG 
TCCCAAAGGG AAGAAATGGT CAAGAAGTGC CAGGCAGAGC TCACAGAACT CTGTAAACGC 
CTGGGCAAAA TATTTGGAGT TCATTACTTC AACATCTTCA ACACGGCCAC AATACGGAGA 
ATTGCAGAAT CACTATCTCC TGAGCCTGAT GTGTTACTTC AGATTGATGG TGTTACAGAG 
GATAAGTTGG AGAAATATGG TGCAGAGCTA ATTGATGTTT TACAGAAATA TTCTGAGTGG 
ACACTTCCAG TGGAAGATAT TCACCAAAAA TCTAGTGGGC CTGCCAATTT CTCTGCAAGG 
AGATATGATA GTGATGATGA TGATGATGAT GATGGTGAGA GAGGTGAAAA GTCGTCTTAC 
TTCAGCACCA AGAGTAATAA AGGTACAAAG CGGAAGAATG CCCCCACCTT TAGGAAGTCC 
AAAAAACGAA AGACTGGAGG AGATGGTCAG CAGTCCCATC CCAAGAATGC GACAGGTTCA 
TTTTCTAGGA ATAATTCTAC TGCCAAAACA TCTAGTAGCT ACTACAGTGG TTCAAAGACA 
GGAGCTGATA AAAGACCTGG ATTTATGGCA CCACCATTGC CTCAGCCCAA CAGACGCTTC 
CTTAAACCAT CCTACACTCT GTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

blm ( NM_001256908.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001243837.1 Xenopus tropicalis Bloom syndrome RecQ like helicase (blm), mRNA.

Price & Availability↑

CloneID	OXa00753
Clone ID Related Accession (Same CDS sequence)	NM_001256908.1
Accession Version	NM_001256908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Bloom syndrome protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX887827.1, DT415557.1, CU024956.1, AAMC03012790.1, CX923823.1, CT025279.2, DR849961.1 and CX907370.1. On Mar 8, 2012 this sequence version replaced XM_002932953.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN04027316, SAMN04027317 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCTC TTCCACAAAA TAATCTTCAG AAGCAGCTAG AACTTTTCTC TGCAAAGGGA 
ACTGCCAACA AGCTTTCTCT TCAGAAACCA AAATCCAGTG TTTTTACCTT TAAAAAGAAA 
TGTTCTCCAA ATGGTTCATT GTCAACTGGC TTCGTTGAAG TTAAGCAAAA TGTTGCTTTA 
AAGGACAAAA ATGTGAATGT GAAACAAAAT GATGTCCAAA CAAACCTCAC AGTTTTGCCA 
AAATCTGCAG AACGCAATAA AATAAATGGA TTTTTCACCC CGGTCTACAC AAAGTCCACA 
CAGCTGCCAC AGACAGTGGC AGTGAAGGAC CACGTCCATA TCAATGACTC TGCAAACAAG 
CACCCAAGAG TGGAGGATTC TGCATCCAAG AAAACAGGGA TTAATACCTC CTTTGGTTCT 
GTTACCAGTT TGGAGGAATG GGATGATCTT GATGATTTTG ACACATCTGT GTCTCCCCCC 
AAGAGTCAGG CTGGCAAAAT AAAAATTACT CCTCAAAATT GCAAGAACAC ATCTCCTGTT 
TCTTCTAAAA TTCAGTGTCT GAGCCCTAAA GGGCCAACCA TTGAAAAGCA GATGTGTGCC 
AAGAAACCAT ATGAGAATAA TGAGATCGCT TCTGAGCCAC AAAAACTTGT GCATGCTCAA 
ACAGCAGAAA TGCCAGACAA ATCATTAGTT TGTTTGGGGT CTGTGGAACA TACAAATTTA 
GGCAGTGCAG AGAAAGACAC ATGTAGGAAC ATTGATTATT CAGGAACTGA TGATATAGAA 
CATGATCAAG AGACGCTTTC ACAGGCACTT ATTGAAAATG AAGATGATTG TGAACCTGAT 
TTTATTCCCC CATCCCCATC AGATGAAAGT CTGTCCTCTC CTCCGGTTTT AAGAGTAATT 
GGTGCACAAA GGAAACACAA AGTCTCTTCT GTAACAGATG TAAATGACTG TGAAAATACA 
GCTGATCACC TGCAGAGCCA ATGTGTCTCT TCTTCACTTG ATTCCAAAGT GCCTTCTCAT 
CTTTTGAGTG TGATGCTTGA AATCTGTGAC TTGGTTGACA AGATTCCAAT ATCAGAGCTA 
CACGTGCTGT CTTGTGGGAT GGACCTCAAG AAAAAAAGGG ACATGCGGAA GCGCCTCTTG 
TTAAATAACT CTGTGTTAAG ATCATCTCCT GCTGACAGCT CAACAGTATC TTTGACTTCC 
TGTCCATCCC AAAATATGGA ATTTGATGTT AGTGCTCCCA AGGATTCAGA AAATTTCTCT 
GGATCTTCTG TGAACAAAAT CTTTAAATTC AATAAATTGG TTGCAGACGA GTTTGGCACA 
AAGGGAAATG ATAGGGCTTC TGATTTTGCT CCCAATTTCA GGGAGAAAAT GGAGAATAAA 
ACCTCTTTCT CTCTCCAGGC TGGAGGAGAT TCAGTGATGG AAGACTCCTT CAGTTTTCAG 
AGCAATGTTC TTAGCACCAG CCAGTTTAAT ACCCCACAAA ATGAGAAGCC AGTCTCATCT 
AGCACCTGCG CCAGACCCAT CAGTCAGCCT ATAGATGACA TGGATAACCC TGATCTTGAC 
TTTGATATTG ATAACTTTGA TATTGAGGAC TTTGATGATC AGCTCATGGA CAGCCCTGCA 
GCTCCTCCTG TTTCCAGCAA AAATGCTCCA CAGTACCCAA CAATATGTGA GACACAGTTT 
GATTCTGGAA GTAAAGAGAA AGTTACACGT CATAATGTCG GTGACAAAAC TAACACAGAG 
TTATCAGACT CTCTACTAAA GCCACAGATA GTGAACCCAG CCCTTGAACG ATTCAAAAGC 
TTTAACTTTC CTCACTCCAA GGAAATGATG AAGATTTTTC ACAAGAAATT TGGTTTGCAT 
CGGTTCCGGA CGAACCAGCT AGAAGCAATT AATGCATGCT TGCTTGGGGA AGACTGCTTC 
ATTCTTATGC CAACAGGTGG TGGGAAAAGT CTATGCTACC AACTGCCAGG CTGTGTATCC 
CCAGGAGTCA CAATTGTCAT CTCTCCCCTC CGGTCCCTGA TTGTAGATCA GGTTCAAAAG 
CTCACCTCTT TGGATGTCTG TGCAAGTACC AGGCTAATAT CAACCATGGA AAATCTATAT 
GAGCGTCAGT TGCTGGCACG ATTTGTCATC GATGAGGCTC ACTGTGTCAG TCAGTGGGGC 
CATGATTTCC GGCCAGACTA CAAGCGTCTG AACATGCTAC GCCAGAAGTT TCAGTCTGTA 
CCAATGATGG CTCTCACAGC AACTGCAAAC CCCCGTGTAC AGAAGGATAT TCTTAATCAA 
CTAAAGATGA CCAAACCTCA AATATTTACA ATGAGCTTTA ACCGAGACAA CCTAAAATAT 
GACGTACTTC CAAAAAAACC CAAGAGGGTG GCATTGGACT GTGTGGAGTG GATCAAGAAA 
TATCACCCAA ATGATTCCGG AATCATTTAT TGTCTGTCAC GACATGAGTG TGATACAATG 
GCAGATACCC TGCAGAAGGG GGGCCTTGCT GCACTTGCAT ATCATGCAGG ACTAGCTGAC 
TCTAACAGAG ATTATGTACA GCATAAGTGG ATAAACCAAG ATGATTGCCA GGTAATCTGT 
GCTACCATTG CCTTTGGAAT GGGCATTGAT AAGCCAGATG TGAGGTATGT CATTCATGCT 
TCTCTACCAA AGTCTGTGGA GGGATATTAC CAGGAGTCAG GGAGAGCTGG CCGAGACGGG 
GAAATATCCC ACTGCCTCCT GTTCTACAGC TACAGTGATG TAACAAGAAT TCGTAGGCTA 
ATACAAATGG AGAAAGATGG AAACAGCCAT ACAAAACAGA CACATTTTAA CAACCTATAC 
AGTATGGTCC ACTACTGTGA AAATGTAGTG GAGTGTCGCA GGATGCAACT CCTGGCTTAT 
TTTGGTGAAA ATAATTTTAA TCCAAACTTC TGCAAAGAGA ACACACGAGT CAGTTGTGAT 
AACTGTCTTG GGAGGAAGGA GTTCAAATCC AGAAATGTGA CCGATGATGT GAGAAACATT 
GTGAGATTTG TGCAAGAAAA TTGCTCTTCA GCACGTGGCA AGGGTAGAAG CAACAGCAGT 
CGACTGACCC TTAACATGAT GGTAGATATA TTCCTAGGTT CTAAGTCTGC AAAAATCCAA 
ACTGGGCTAT TTGGGAAAGG AGCTGCTTAT TCACGGCACA ATGCAGAACG TCTCTTCCGG 
AAGTTAGTCC TGGACAGGAT ATTAGATGAA GAGCTTTACA TTACTGCCAA TGATCAGGCT 
GTGGCCTATG TAAAAACAGG AGAGCGGGCG CAGGCTGTGC TAAATGGATT CTTGACGGTT 
GAGTTCCAAG ACACCGAAAA TGCCTCCAGC CTCAGAAAGC AAAAGGCATC AGTTGTCGCT 
AATACGTCCC AAAGGGAAGA AATGGTCAAG AAGTGCCAGG CAGAGCTCAC AGAACTCTGT 
AAACGCCTGG GCAAAATATT TGGAGTTCAT TACTTCAACA TCTTCAACAC GGCCACAATA 
CGGAGAATTG CAGAATCACT ATCTCCTGAG CCTGATGTGT TACTTCAGAT TGATGGTGTT 
ACAGAGGATA AGTTGGAGAA ATATGGTGCA GAGCTAATTG ATGTTTTACA GAAATATTCT 
GAGTGGACAC TTCCAGTGGA AGATATTCAC CAAAAATCTA GTGGGCCTGC CAATTTCTCT 
GCAAGGAGAT ATGATAGTGA TGATGATGAT GGTGAGAGAG GTGAAAAGTC GTCTTACTTC 
AGCACCAAGA GTAATAAAGG TACAAAGCGG AAGAATGCCC CCACCTTTAG GAAGTCCAAA 
AAACGAAAGA CTGGAGGAGA TGGTCAGCAG TCCCATCCCA AGAATGCGAC AGGTTCATTT 
TCTAGGAATA ATTCTACTGC CAAAACATCT AGTAGCTACT ACAGTGGTTC AAAGACAGGA 
GCTGATAAAA GACCTGGATT TATGGCACCA CCATTGCCTC AGCCCAACAG ACGCTTCCTT 
AAACCATCCT ACACTCTGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243837.1
CDS	443..4426
Translation	MAALPQNNLQKQLELFSAKGTANKLSLQKPKSSVFTFKKKCSPNGSLSTGFVEVKQNVALKDKNVNVKQNDVQTNLTVLPKSAERNKINGFFTPVYTKSTQLPQTVAVKDHVHINDSANKHPRVEDSASKKTGINTSFGSVTSLEEWDDLDDFDTSVSPPKSQAGKIKITPQNCKNTSPVSSKIQCLSPKGPTIEKQMCAKKPYENNEIASEPQKLVHAQTAEMPDKSLVCLGSVEHTNLGSAEKDTCRNIDYSGTDDIEHDQETLSQALIENEDDCEPDFIPPSPSDESLSSPPVLRVIGAQRKHKVSSVTDVNDCENTADHLQSQCVSSSLDSKVPSHLLSVMLEICDLVDKIPISELHVLSCGMDLKKKRDMRKRLLLNNSVLRSSPADSSTVSLTSCPSQNMEFDVSAPKDSENFSGSSVNKIFKFNKLVADEFGTKGNDRASDFAPNFREKMENKTSFSLQAGGDSVMEDSFSFQSNVLSTSQFNTPQNEKPVSSSTCARPISQPIDDMDNPDLDFDIDNFDIEDFDDQLMDSPAAPPVSSKNAPQYPTICETQFDSGSKEKVTRHNVGDKTNTELSDSLLKPQIVNPALERFKSFNFPHSKEMMKIFHKKFGLHRFRTNQLEAINACLLGEDCFILMPTGGGKSLCYQLPGCVSPGVTIVISPLRSLIVDQVQKLTSLDVCASTRLISTMENLYERQLLARFVIDEAHCVSQWGHDFRPDYKRLNMLRQKFQSVPMMALTATANPRVQKDILNQLKMTKPQIFTMSFNRDNLKYDVLPKKPKRVALDCVEWIKKYHPNDSGIIYCLSRHECDTMADTLQKGGLAALAYHAGLADSNRDYVQHKWINQDDCQVICATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYSYSDVTRIRRLIQMEKDGNSHTKQTHFNNLYSMVHYCENVVECRRMQLLAYFGENNFNPNFCKENTRVSCDNCLGRKEFKSRNVTDDVRNIVRFVQENCSSARGKGRSNSSRLTLNMMVDIFLGSKSAKIQTGLFGKGAAYSRHNAERLFRKLVLDRILDEELYITANDQAVAYVKTGERAQAVLNGFLTVEFQDTENASSLRKQKASVVANTSQREEMVKKCQAELTELCKRLGKIFGVHYFNIFNTATIRRIAESLSPEPDVLLQIDGVTEDKLEKYGAELIDVLQKYSEWTLPVEDIHQKSSGPANFSARRYDSDDDDGERGEKSSYFSTKSNKGTKRKNAPTFRKSKKRKTGGDGQQSHPKNATGSFSRNNSTAKTSSSYYSGSKTGADKRPGFMAPPLPQPNRRFLKPSYTLF

Target ORF information:

RefSeq Version	NM_001256908.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Bloom syndrome RecQ like helicase (blm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256908.1

ORF Insert Sequence:

ATGGCTGCTC TTCCACAAAA TAATCTTCAG AAGCAGCTAG AACTTTTCTC TGCAAAGGGA 
ACTGCCAACA AGCTTTCTCT TCAGAAACCA AAATCCAGTG TTTTTACCTT TAAAAAGAAA 
TGTTCTCCAA ATGGTTCATT GTCAACTGGC TTCGTTGAAG TTAAGCAAAA TGTTGCTTTA 
AAGGACAAAA ATGTGAATGT GAAACAAAAT GATGTCCAAA CAAACCTCAC AGTTTTGCCA 
AAATCTGCAG AACGCAATAA AATAAATGGA TTTTTCACCC CGGTCTACAC AAAGTCCACA 
CAGCTGCCAC AGACAGTGGC AGTGAAGGAC CACGTCCATA TCAATGACTC TGCAAACAAG 
CACCCAAGAG TGGAGGATTC TGCATCCAAG AAAACAGGGA TTAATACCTC CTTTGGTTCT 
GTTACCAGTT TGGAGGAATG GGATGATCTT GATGATTTTG ACACATCTGT GTCTCCCCCC 
AAGAGTCAGG CTGGCAAAAT AAAAATTACT CCTCAAAATT GCAAGAACAC ATCTCCTGTT 
TCTTCTAAAA TTCAGTGTCT GAGCCCTAAA GGGCCAACCA TTGAAAAGCA GATGTGTGCC 
AAGAAACCAT ATGAGAATAA TGAGATCGCT TCTGAGCCAC AAAAACTTGT GCATGCTCAA 
ACAGCAGAAA TGCCAGACAA ATCATTAGTT TGTTTGGGGT CTGTGGAACA TACAAATTTA 
GGCAGTGCAG AGAAAGACAC ATGTAGGAAC ATTGATTATT CAGGAACTGA TGATATAGAA 
CATGATCAAG AGACGCTTTC ACAGGCACTT ATTGAAAATG AAGATGATTG TGAACCTGAT 
TTTATTCCCC CATCCCCATC AGATGAAAGT CTGTCCTCTC CTCCGGTTTT AAGAGTAATT 
GGTGCACAAA GGAAACACAA AGTCTCTTCT GTAACAGATG TAAATGACTG TGAAAATACA 
GCTGATCACC TGCAGAGCCA ATGTGTCTCT TCTTCACTTG ATTCCAAAGT GCCTTCTCAT 
CTTTTGAGTG TGATGCTTGA AATCTGTGAC TTGGTTGACA AGATTCCAAT ATCAGAGCTA 
CACGTGCTGT CTTGTGGGAT GGACCTCAAG AAAAAAAGGG ACATGCGGAA GCGCCTCTTG 
TTAAATAACT CTGTGTTAAG ATCATCTCCT GCTGACAGCT CAACAGTATC TTTGACTTCC 
TGTCCATCCC AAAATATGGA ATTTGATGTT AGTGCTCCCA AGGATTCAGA AAATTTCTCT 
GGATCTTCTG TGAACAAAAT CTTTAAATTC AATAAATTGG TTGCAGACGA GTTTGGCACA 
AAGGGAAATG ATAGGGCTTC TGATTTTGCT CCCAATTTCA GGGAGAAAAT GGAGAATAAA 
ACCTCTTTCT CTCTCCAGGC TGGAGGAGAT TCAGTGATGG AAGACTCCTT CAGTTTTCAG 
AGCAATGTTC TTAGCACCAG CCAGTTTAAT ACCCCACAAA ATGAGAAGCC AGTCTCATCT 
AGCACCTGCG CCAGACCCAT CAGTCAGCCT ATAGATGACA TGGATAACCC TGATCTTGAC 
TTTGATATTG ATAACTTTGA TATTGAGGAC TTTGATGATC AGCTCATGGA CAGCCCTGCA 
GCTCCTCCTG TTTCCAGCAA AAATGCTCCA CAGTACCCAA CAATATGTGA GACACAGTTT 
GATTCTGGAA GTAAAGAGAA AGTTACACGT CATAATGTCG GTGACAAAAC TAACACAGAG 
TTATCAGACT CTCTACTAAA GCCACAGATA GTGAACCCAG CCCTTGAACG ATTCAAAAGC 
TTTAACTTTC CTCACTCCAA GGAAATGATG AAGATTTTTC ACAAGAAATT TGGTTTGCAT 
CGGTTCCGGA CGAACCAGCT AGAAGCAATT AATGCATGCT TGCTTGGGGA AGACTGCTTC 
ATTCTTATGC CAACAGGTGG TGGGAAAAGT CTATGCTACC AACTGCCAGG CTGTGTATCC 
CCAGGAGTCA CAATTGTCAT CTCTCCCCTC CGGTCCCTGA TTGTAGATCA GGTTCAAAAG 
CTCACCTCTT TGGATGTCTG TGCAAGTACC AGGCTAATAT CAACCATGGA AAATCTATAT 
GAGCGTCAGT TGCTGGCACG ATTTGTCATC GATGAGGCTC ACTGTGTCAG TCAGTGGGGC 
CATGATTTCC GGCCAGACTA CAAGCGTCTG AACATGCTAC GCCAGAAGTT TCAGTCTGTA 
CCAATGATGG CTCTCACAGC AACTGCAAAC CCCCGTGTAC AGAAGGATAT TCTTAATCAA 
CTAAAGATGA CCAAACCTCA AATATTTACA ATGAGCTTTA ACCGAGACAA CCTAAAATAT 
GACGTACTTC CAAAAAAACC CAAGAGGGTG GCATTGGACT GTGTGGAGTG GATCAAGAAA 
TATCACCCAA ATGATTCCGG AATCATTTAT TGTCTGTCAC GACATGAGTG TGATACAATG 
GCAGATACCC TGCAGAAGGG GGGCCTTGCT GCACTTGCAT ATCATGCAGG ACTAGCTGAC 
TCTAACAGAG ATTATGTACA GCATAAGTGG ATAAACCAAG ATGATTGCCA GGTAATCTGT 
GCTACCATTG CCTTTGGAAT GGGCATTGAT AAGCCAGATG TGAGGTATGT CATTCATGCT 
TCTCTACCAA AGTCTGTGGA GGGATATTAC CAGGAGTCAG GGAGAGCTGG CCGAGACGGG 
GAAATATCCC ACTGCCTCCT GTTCTACAGC TACAGTGATG TAACAAGAAT TCGTAGGCTA 
ATACAAATGG AGAAAGATGG AAACAGCCAT ACAAAACAGA CACATTTTAA CAACCTATAC 
AGTATGGTCC ACTACTGTGA AAATGTAGTG GAGTGTCGCA GGATGCAACT CCTGGCTTAT 
TTTGGTGAAA ATAATTTTAA TCCAAACTTC TGCAAAGAGA ACACACGAGT CAGTTGTGAT 
AACTGTCTTG GGAGGAAGGA GTTCAAATCC AGAAATGTGA CCGATGATGT GAGAAACATT 
GTGAGATTTG TGCAAGAAAA TTGCTCTTCA GCACGTGGCA AGGGTAGAAG CAACAGCAGT 
CGACTGACCC TTAACATGAT GGTAGATATA TTCCTAGGTT CTAAGTCTGC AAAAATCCAA 
ACTGGGCTAT TTGGGAAAGG AGCTGCTTAT TCACGGCACA ATGCAGAACG TCTCTTCCGG 
AAGTTAGTCC TGGACAGGAT ATTAGATGAA GAGCTTTACA TTACTGCCAA TGATCAGGCT 
GTGGCCTATG TAAAAACAGG AGAGCGGGCG CAGGCTGTGC TAAATGGATT CTTGACGGTT 
GAGTTCCAAG ACACCGAAAA TGCCTCCAGC CTCAGAAAGC AAAAGGCATC AGTTGTCGCT 
AATACGTCCC AAAGGGAAGA AATGGTCAAG AAGTGCCAGG CAGAGCTCAC AGAACTCTGT 
AAACGCCTGG GCAAAATATT TGGAGTTCAT TACTTCAACA TCTTCAACAC GGCCACAATA 
CGGAGAATTG CAGAATCACT ATCTCCTGAG CCTGATGTGT TACTTCAGAT TGATGGTGTT 
ACAGAGGATA AGTTGGAGAA ATATGGTGCA GAGCTAATTG ATGTTTTACA GAAATATTCT 
GAGTGGACAC TTCCAGTGGA AGATATTCAC CAAAAATCTA GTGGGCCTGC CAATTTCTCT 
GCAAGGAGAT ATGATAGTGA TGATGATGAT GGTGAGAGAG GTGAAAAGTC GTCTTACTTC 
AGCACCAAGA GTAATAAAGG TACAAAGCGG AAGAATGCCC CCACCTTTAG GAAGTCCAAA 
AAACGAAAGA CTGGAGGAGA TGGTCAGCAG TCCCATCCCA AGAATGCGAC AGGTTCATTT 
TCTAGGAATA ATTCTACTGC CAAAACATCT AGTAGCTACT ACAGTGGTTC AAAGACAGGA 
GCTGATAAAA GACCTGGATT TATGGCACCA CCATTGCCTC AGCCCAACAG ACGCTTCCTT 
AAACCATCCT ACACTCTGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

blm ( XM_012958409.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813863.1 Xenopus tropicalis Bloom syndrome, RecQ helicase-like (blm), transcript variant X1, mRNA.

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CloneID	OXa30300
Clone ID Related Accession (Same CDS sequence)	XM_012958409.2
Accession Version	XM_012958409.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Bloom syndrome protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958409.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTC TTCCACAAAA TAATCTTCAG AAGCAGCTAG AACTTTTCTC TGCAAAGGGA 
ACTGCCAACA AGCTTTCTCT TCAGAAACCA AAATCCAGTG TTTTTACCTT TAAAAAGAAA 
TGTTCTCCAA ATGGTTCATT GTCAACTGGC TTCGTTGAAG TTAAGCAAAA TGTTGCTTTA 
AAGGACAAAA ATGTGAATGT GAAACAAAAT GATGTCCAAA CAAACCTCAC AGTTTTGCCA 
AAATCTGCAG AACGCAATAA AATAAATGGA TTTTTCACCC CGGTCTACAC AAAGTCCACA 
CAGCTGCCAC AGACAGTGGC AGTGAAGGAC CACGTCCATA TCAATGACTC TGCAAACAAG 
CACCCAAGAG TGGAGGATTC TGCATCCAAG AAAACAGGGA TTAATACCTC CTTTGGTTCT 
GTTACCAGTT TGGAGGAATG GGATGATCTT GATGATTTTG ACACATCTGT GTCTCCCCCC 
AAGAGTCAGG CTGGCAAAAT AAAAATTACT CCTCAAAATT GCAAGAACAC ATCTCCTGTT 
TCTTCTAAAA TTCAGTGTCT GAGCCCTAAA GGGCCAACCA TTGAAAAGCA GATGTGTGCC 
AAGAAACCAT ATGAGAATAA TGAGATCGCT TCTGAGCCAC AAAAACTTGT GCATGCTCAA 
ACAGCAGAAA TGCCAGACAA ATCATTAGTT TGTTTGGGGT CTGTGGAACA TACAAATTTA 
GGCAGTGCAG AGAAAGACAC ATGTAGGAAC ATTGATTATT CAGGAACTGA TGATATAGAA 
CATGATCAAG AGACGCTTTC ACAGGCACTT ATTGAAAATG AAGATGATTG TGAACCTGAT 
TTTATTCCCC CATCCCCATC AGATGAAAGT CTGTCCTCTC CTCCGGTTTT AAGAGTAATT 
GGTGCACAAA GGAAACACAA AGTCTCTTCT GTAACAGATG TAAATGACTG TGAAAATACA 
GCTGATCACC TGCAGAGCCA ATGTGTCTCT TCTTCACTTG ATTCCAAAGT GCCTTCTCAT 
CTTTTGAGTG TGATGCTTGA AATCTGTGAC TTGGTTGACA AGATTCCAAT ATCAGAGCTA 
CACGTGCTGT CTTGTGGGAT GGACCTCAAG AAAAAAAGGG ACATGCGGAA GCGCCTCTTG 
TTAAATAACT CTGTGTTAAG ATCATCTCCT GCTGACAGCT CAACAGTATC TTTGACTTCC 
TGTCCATCCC AAAATATGGA ATTTGATGTT AGTGCTCCCA AGGATTCAGA AAATTTCTCT 
GGATCTTCTG TGAACAAAAT CTTTAAATTC AATAAATTGG TTGCAGACGA GTTTGGCACA 
AAGGGAAATG ATAGGGCTTC TGATTTTGCT CCCAATTTCA GGGAGAAAAT GGAGAATAAA 
ACCTCTTTCT CTCTCCAGGC TGGAGGAGAT TCAGTGATGG AAGACTCCTT CAGTTTTCAG 
AGCAATGTTC TTAGCACCAG CCAGTTTAAT ACCCCACAAA ATGAGAAGCC AGTCTCATCT 
AGCACCTGCG CCAGACCCAT CAGTCAGCCT ATAGATGACA TGGATAACCC TGATCTTGAC 
TTTGATATTG ATAACTTTGA TATTGAGGAC TTTGATGATC AGCTCATGGA CAGCCCTGCA 
GCTCCTCCTG TTTCCAGCAA AAATGCTCCA CAGTACCCAA CAATATGTGA GACACAGTTT 
GATTCTGGAA GTAAAGAGAA AGTTACACGT CATAATGTCG GTGACAAAAC TAACACAGAG 
TTATCAGACT CTCTACTAAA GCCACAGATA GTGAACCCAG CCCTTGAACG ATTCAAAAGC 
TTTAACTTTC CTCACTCCAA GGAAATGATG AAGATTTTTC ACAAGAAATT TGGTTTGCAT 
CGGTTCCGGA CGAACCAGCT AGAAGCAATT AATGCATGCT TGCTTGGGGA AGACTGCTTC 
ATTCTTATGC CAACAGGTGG TGGGAAAAGT CTATGCTACC AACTGCCAGG CTGTGTATCC 
CCAGGAGTCA CAATTGTCAT CTCTCCCCTC CGGTCCCTGA TTGTAGATCA GGTTCAAAAG 
CTCACCTCTT TGGATATTCC AGCTACATAT CTCACTGGAG ATAAGACAGA TGCTGAAGCT 
GCTAGTATTT ATTTGCAACT CTCTAAGAAA GACCCTATCA TTAAACTTCT CTATGTCACT 
CCAGAAAAGG TCTGTGCAAG TACCAGGCTA ATATCAACCA TGGAAAATCT ATATGAGCGT 
CAGTTGCTGG CACGATTTGT CATCGATGAG GCTCACTGTG TCAGTCAGTG GGGCCATGAT 
TTCCGGCCAG ACTACAAGCG TCTGAACATG CTACGCCAGA AGTTTCAGTC TGTACCAATG 
ATGGCTCTCA CAGCAACTGC AAACCCCCGT GTACAGAAGG ATATTCTTAA TCAACTAAAG 
ATGACCAAAC CTCAAATATT TACAATGAGC TTTAACCGAG ACAACCTAAA ATATGACGTA 
CTTCCAAAAA AACCCAAGAG GGTGGCATTG GACTGTGTGG AGTGGATCAA GAAATATCAC 
CCAAATGATT CCGGAATCAT TTATTGTCTG TCACGACATG AGTGTGATAC AATGGCAGAT 
ACCCTGCAGA AGGGGGGCCT TGCTGCACTT GCATATCATG CAGGACTAGC TGACTCTAAC 
AGAGATTATG TACAGCATAA GTGGATAAAC CAAGATGATT GCCAGGTAAT CTGTGCTACC 
ATTGCCTTTG GAATGGGCAT TGATAAGCCA GATGTGAGGT ATGTCATTCA TGCTTCTCTA 
CCAAAGTCTG TGGAGGGATA TTACCAGGAG TCAGGGAGAG CTGGCCGAGA CGGGGAAATA 
TCCCACTGCC TCCTGTTCTA CAGCTACAGT GATGTAACAA GAATTCGTAG GCTAATACAA 
ATGGAGAAAG ATGGAAACAG CCATACAAAA CAGACACATT TTAACAACCT ATACAGTATG 
GTCCACTACT GTGAAAATGT AGTGGAGTGT CGCAGGATGC AACTCCTGGC TTATTTTGGT 
GAAAATAATT TTAATCCAAA CTTCTGCAAA GAGAACACAC GAGTCAGTTG TGATAACTGT 
CTTGGGAGGA AGGAGTTCAA ATCCAGAAAT GTGACCGATG ATGTGAGAAA CATTGTGAGA 
TTTGTGCAAG AAAATTGCTC TTCAGCACGT GGCAAGGGTA GAAGCAACAG CAGTCGACTG 
ACCCTTAACA TGATGGTAGA TATATTCCTA GGTTCTAAGT CTGCAAAAAT CCAAACTGGG 
CTATTTGGGA AAGGAGCTGC TTATTCACGG CACAATGCAG AACGTCTCTT CCGGAAGTTA 
GTCCTGGACA GGATATTAGA TGAAGAGCTT TACATTACTG CCAATGATCA GGCTGTGGCC 
TATGTAAAAA CAGGAGAGCG GGCGCAGGCT GTGCTAAATG GATTCTTGAC GGTTGAGTTC 
CAAGACACCG AAAATGCCTC CAGCCTCAGA AAGCAAAAGG CATCAGTTGT CGCTAATACG 
TCCCAAAGGG AAGAAATGGT CAAGAAGTGC CAGGCAGAGC TCACAGAACT CTGTAAACGC 
CTGGGCAAAA TATTTGGAGT TCATTACTTC AACATCTTCA ACACGGCCAC AATACGGAGA 
ATTGCAGAAT CACTATCTCC TGAGCCTGAT GTGTTACTTC AGATTGATGG TGTTACAGAG 
GATAAGTTGG AGAAATATGG TGCAGAGCTA ATTGATGTTT TACAGAAATA TTCTGAGTGG 
ACACTTCCAG TGGAAGATAT TCACCAAAAA TCTAGTGGGC CTGCCAATTT CTCTGCAAGG 
AGATATGATA GTGATGATGA TGATGGTGAG AGAGGTGAAA AGTCGTCTTA CTTCAGCACC 
AAGAGTAATA AAGGTACAAA GCGGAAGAAT GCCCCCACCT TTAGGAAGTC CAAAAAACGA 
AAGACTGGAG GAGATGGTCA GCAGTCCCAT CCCAAGAATG CGACAGGTTC ATTTTCTAGG 
AATAATTCTA CTGCCAAAAC ATCTAGTAGC TACTACAGTG GTTCAAAGAC AGGAGCTGAT 
AAAAGACCTG GATTTATGGC ACCACCATTG CCTCAGCCCA ACAGACGCTT CCTTAAACCA 
TCCTACACTC TGTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813863.1
CDS	438..4535
Translation	MAALPQNNLQKQLELFSAKGTANKLSLQKPKSSVFTFKKKCSPNGSLSTGFVEVKQNVALKDKNVNVKQNDVQTNLTVLPKSAERNKINGFFTPVYTKSTQLPQTVAVKDHVHINDSANKHPRVEDSASKKTGINTSFGSVTSLEEWDDLDDFDTSVSPPKSQAGKIKITPQNCKNTSPVSSKIQCLSPKGPTIEKQMCAKKPYENNEIASEPQKLVHAQTAEMPDKSLVCLGSVEHTNLGSAEKDTCRNIDYSGTDDIEHDQETLSQALIENEDDCEPDFIPPSPSDESLSSPPVLRVIGAQRKHKVSSVTDVNDCENTADHLQSQCVSSSLDSKVPSHLLSVMLEICDLVDKIPISELHVLSCGMDLKKKRDMRKRLLLNNSVLRSSPADSSTVSLTSCPSQNMEFDVSAPKDSENFSGSSVNKIFKFNKLVADEFGTKGNDRASDFAPNFREKMENKTSFSLQAGGDSVMEDSFSFQSNVLSTSQFNTPQNEKPVSSSTCARPISQPIDDMDNPDLDFDIDNFDIEDFDDQLMDSPAAPPVSSKNAPQYPTICETQFDSGSKEKVTRHNVGDKTNTELSDSLLKPQIVNPALERFKSFNFPHSKEMMKIFHKKFGLHRFRTNQLEAINACLLGEDCFILMPTGGGKSLCYQLPGCVSPGVTIVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDAEAASIYLQLSKKDPIIKLLYVTPEKVCASTRLISTMENLYERQLLARFVIDEAHCVSQWGHDFRPDYKRLNMLRQKFQSVPMMALTATANPRVQKDILNQLKMTKPQIFTMSFNRDNLKYDVLPKKPKRVALDCVEWIKKYHPNDSGIIYCLSRHECDTMADTLQKGGLAALAYHAGLADSNRDYVQHKWINQDDCQVICATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYSYSDVTRIRRLIQMEKDGNSHTKQTHFNNLYSMVHYCENVVECRRMQLLAYFGENNFNPNFCKENTRVSCDNCLGRKEFKSRNVTDDVRNIVRFVQENCSSARGKGRSNSSRLTLNMMVDIFLGSKSAKIQTGLFGKGAAYSRHNAERLFRKLVLDRILDEELYITANDQAVAYVKTGERAQAVLNGFLTVEFQDTENASSLRKQKASVVANTSQREEMVKKCQAELTELCKRLGKIFGVHYFNIFNTATIRRIAESLSPEPDVLLQIDGVTEDKLEKYGAELIDVLQKYSEWTLPVEDIHQKSSGPANFSARRYDSDDDDGERGEKSSYFSTKSNKGTKRKNAPTFRKSKKRKTGGDGQQSHPKNATGSFSRNNSTAKTSSSYYSGSKTGADKRPGFMAPPLPQPNRRFLKPSYTLF

Target ORF information:

RefSeq Version	XM_012958409.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Bloom syndrome, RecQ helicase-like (blm), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958409.2

ORF Insert Sequence:

ATGGCTGCTC TTCCACAAAA TAATCTTCAG AAGCAGCTAG AACTTTTCTC TGCAAAGGGA 
ACTGCCAACA AGCTTTCTCT TCAGAAACCA AAATCCAGTG TTTTTACCTT TAAAAAGAAA 
TGTTCTCCAA ATGGTTCATT GTCAACTGGC TTCGTTGAAG TTAAGCAAAA TGTTGCTTTA 
AAGGACAAAA ATGTGAATGT GAAACAAAAT GATGTCCAAA CAAACCTCAC AGTTTTGCCA 
AAATCTGCAG AACGCAATAA AATAAATGGA TTTTTCACCC CGGTCTACAC AAAGTCCACA 
CAGCTGCCAC AGACAGTGGC AGTGAAGGAC CACGTCCATA TCAATGACTC TGCAAACAAG 
CACCCAAGAG TGGAGGATTC TGCATCCAAG AAAACAGGGA TTAATACCTC CTTTGGTTCT 
GTTACCAGTT TGGAGGAATG GGATGATCTT GATGATTTTG ACACATCTGT GTCTCCCCCC 
AAGAGTCAGG CTGGCAAAAT AAAAATTACT CCTCAAAATT GCAAGAACAC ATCTCCTGTT 
TCTTCTAAAA TTCAGTGTCT GAGCCCTAAA GGGCCAACCA TTGAAAAGCA GATGTGTGCC 
AAGAAACCAT ATGAGAATAA TGAGATCGCT TCTGAGCCAC AAAAACTTGT GCATGCTCAA 
ACAGCAGAAA TGCCAGACAA ATCATTAGTT TGTTTGGGGT CTGTGGAACA TACAAATTTA 
GGCAGTGCAG AGAAAGACAC ATGTAGGAAC ATTGATTATT CAGGAACTGA TGATATAGAA 
CATGATCAAG AGACGCTTTC ACAGGCACTT ATTGAAAATG AAGATGATTG TGAACCTGAT 
TTTATTCCCC CATCCCCATC AGATGAAAGT CTGTCCTCTC CTCCGGTTTT AAGAGTAATT 
GGTGCACAAA GGAAACACAA AGTCTCTTCT GTAACAGATG TAAATGACTG TGAAAATACA 
GCTGATCACC TGCAGAGCCA ATGTGTCTCT TCTTCACTTG ATTCCAAAGT GCCTTCTCAT 
CTTTTGAGTG TGATGCTTGA AATCTGTGAC TTGGTTGACA AGATTCCAAT ATCAGAGCTA 
CACGTGCTGT CTTGTGGGAT GGACCTCAAG AAAAAAAGGG ACATGCGGAA GCGCCTCTTG 
TTAAATAACT CTGTGTTAAG ATCATCTCCT GCTGACAGCT CAACAGTATC TTTGACTTCC 
TGTCCATCCC AAAATATGGA ATTTGATGTT AGTGCTCCCA AGGATTCAGA AAATTTCTCT 
GGATCTTCTG TGAACAAAAT CTTTAAATTC AATAAATTGG TTGCAGACGA GTTTGGCACA 
AAGGGAAATG ATAGGGCTTC TGATTTTGCT CCCAATTTCA GGGAGAAAAT GGAGAATAAA 
ACCTCTTTCT CTCTCCAGGC TGGAGGAGAT TCAGTGATGG AAGACTCCTT CAGTTTTCAG 
AGCAATGTTC TTAGCACCAG CCAGTTTAAT ACCCCACAAA ATGAGAAGCC AGTCTCATCT 
AGCACCTGCG CCAGACCCAT CAGTCAGCCT ATAGATGACA TGGATAACCC TGATCTTGAC 
TTTGATATTG ATAACTTTGA TATTGAGGAC TTTGATGATC AGCTCATGGA CAGCCCTGCA 
GCTCCTCCTG TTTCCAGCAA AAATGCTCCA CAGTACCCAA CAATATGTGA GACACAGTTT 
GATTCTGGAA GTAAAGAGAA AGTTACACGT CATAATGTCG GTGACAAAAC TAACACAGAG 
TTATCAGACT CTCTACTAAA GCCACAGATA GTGAACCCAG CCCTTGAACG ATTCAAAAGC 
TTTAACTTTC CTCACTCCAA GGAAATGATG AAGATTTTTC ACAAGAAATT TGGTTTGCAT 
CGGTTCCGGA CGAACCAGCT AGAAGCAATT AATGCATGCT TGCTTGGGGA AGACTGCTTC 
ATTCTTATGC CAACAGGTGG TGGGAAAAGT CTATGCTACC AACTGCCAGG CTGTGTATCC 
CCAGGAGTCA CAATTGTCAT CTCTCCCCTC CGGTCCCTGA TTGTAGATCA GGTTCAAAAG 
CTCACCTCTT TGGATATTCC AGCTACATAT CTCACTGGAG ATAAGACAGA TGCTGAAGCT 
GCTAGTATTT ATTTGCAACT CTCTAAGAAA GACCCTATCA TTAAACTTCT CTATGTCACT 
CCAGAAAAGG TCTGTGCAAG TACCAGGCTA ATATCAACCA TGGAAAATCT ATATGAGCGT 
CAGTTGCTGG CACGATTTGT CATCGATGAG GCTCACTGTG TCAGTCAGTG GGGCCATGAT 
TTCCGGCCAG ACTACAAGCG TCTGAACATG CTACGCCAGA AGTTTCAGTC TGTACCAATG 
ATGGCTCTCA CAGCAACTGC AAACCCCCGT GTACAGAAGG ATATTCTTAA TCAACTAAAG 
ATGACCAAAC CTCAAATATT TACAATGAGC TTTAACCGAG ACAACCTAAA ATATGACGTA 
CTTCCAAAAA AACCCAAGAG GGTGGCATTG GACTGTGTGG AGTGGATCAA GAAATATCAC 
CCAAATGATT CCGGAATCAT TTATTGTCTG TCACGACATG AGTGTGATAC AATGGCAGAT 
ACCCTGCAGA AGGGGGGCCT TGCTGCACTT GCATATCATG CAGGACTAGC TGACTCTAAC 
AGAGATTATG TACAGCATAA GTGGATAAAC CAAGATGATT GCCAGGTAAT CTGTGCTACC 
ATTGCCTTTG GAATGGGCAT TGATAAGCCA GATGTGAGGT ATGTCATTCA TGCTTCTCTA 
CCAAAGTCTG TGGAGGGATA TTACCAGGAG TCAGGGAGAG CTGGCCGAGA CGGGGAAATA 
TCCCACTGCC TCCTGTTCTA CAGCTACAGT GATGTAACAA GAATTCGTAG GCTAATACAA 
ATGGAGAAAG ATGGAAACAG CCATACAAAA CAGACACATT TTAACAACCT ATACAGTATG 
GTCCACTACT GTGAAAATGT AGTGGAGTGT CGCAGGATGC AACTCCTGGC TTATTTTGGT 
GAAAATAATT TTAATCCAAA CTTCTGCAAA GAGAACACAC GAGTCAGTTG TGATAACTGT 
CTTGGGAGGA AGGAGTTCAA ATCCAGAAAT GTGACCGATG ATGTGAGAAA CATTGTGAGA 
TTTGTGCAAG AAAATTGCTC TTCAGCACGT GGCAAGGGTA GAAGCAACAG CAGTCGACTG 
ACCCTTAACA TGATGGTAGA TATATTCCTA GGTTCTAAGT CTGCAAAAAT CCAAACTGGG 
CTATTTGGGA AAGGAGCTGC TTATTCACGG CACAATGCAG AACGTCTCTT CCGGAAGTTA 
GTCCTGGACA GGATATTAGA TGAAGAGCTT TACATTACTG CCAATGATCA GGCTGTGGCC 
TATGTAAAAA CAGGAGAGCG GGCGCAGGCT GTGCTAAATG GATTCTTGAC GGTTGAGTTC 
CAAGACACCG AAAATGCCTC CAGCCTCAGA AAGCAAAAGG CATCAGTTGT CGCTAATACG 
TCCCAAAGGG AAGAAATGGT CAAGAAGTGC CAGGCAGAGC TCACAGAACT CTGTAAACGC 
CTGGGCAAAA TATTTGGAGT TCATTACTTC AACATCTTCA ACACGGCCAC AATACGGAGA 
ATTGCAGAAT CACTATCTCC TGAGCCTGAT GTGTTACTTC AGATTGATGG TGTTACAGAG 
GATAAGTTGG AGAAATATGG TGCAGAGCTA ATTGATGTTT TACAGAAATA TTCTGAGTGG 
ACACTTCCAG TGGAAGATAT TCACCAAAAA TCTAGTGGGC CTGCCAATTT CTCTGCAAGG 
AGATATGATA GTGATGATGA TGATGGTGAG AGAGGTGAAA AGTCGTCTTA CTTCAGCACC 
AAGAGTAATA AAGGTACAAA GCGGAAGAAT GCCCCCACCT TTAGGAAGTC CAAAAAACGA 
AAGACTGGAG GAGATGGTCA GCAGTCCCAT CCCAAGAATG CGACAGGTTC ATTTTCTAGG 
AATAATTCTA CTGCCAAAAC ATCTAGTAGC TACTACAGTG GTTCAAAGAC AGGAGCTGAT 
AAAAGACCTG GATTTATGGC ACCACCATTG CCTCAGCCCA ACAGACGCTT CCTTAAACCA 
TCCTACACTC TGTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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blm cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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