Goal: Generate rabbit monoclonal antibodies that are specific to Claudin 18.2 but not recognizing Claudin 18.1
Immunogen: DNA + Protein
Result: Successfully generated 47 Claudin 18.2-positive and Claudin 18.1-negative clones; among these clone 8 performed well in IF and IHC assays.
|Screening Methods||Indirect ELISA||Cell-Based ELISA||Cell-Based IHC||FACS||FACS|
|Clone ID||Claudin 18.2 protein||Vero-Claudin18.2||Vero-Claudin 18.2||CHO-K1-Claudin 18.1|
IF Results of Clone 8
The fluorescent staining could be observed on the Vero-18.2 samples for both supernatant and purified antibody of the Clone 8.
IHC Results of Clone 8
The membrane staining on paraffin-embedded tissue sections of gastric cancer could be observed.