Gene Symbol | TUFM |
Entrez Gene ID | 281556 |
Full Name | Tu translation elongation factor, mitochondrial |
General protein information |
|
Gene Type | protein-coding |
Organism | Bos taurus(cattle) |
ORF » Species Summary » Bos taurus » TUFM cDNA ORF clone
Gene Symbol | TUFM |
Entrez Gene ID | 281556 |
Full Name | Tu translation elongation factor, mitochondrial |
General protein information |
|
Gene Type | protein-coding |
Organism | Bos taurus(cattle) |
mRNA | Protein | Name |
---|---|---|
NM_174207.2 | NP_776632.1 | elongation factor Tu, mitochondrial precursor |
TUFM | XP_001103519.1 | |
Homo sapiens (human) | TUFM | NP_003312.3 |
Saccharomyces cerevisiae (baker's yeast) | TUF1 | NP_014830.1 |
Kluyveromyces lactis | KLLA0A04587g | XP_451201.1 |
Kluyveromyces lactis | KLLA0C05214g | XP_452433.1 |
Eremothecium gossypii | AGOS_AAR143W | NP_982685.1 |
Magnaporthe oryzae (rice blast fungus) | MGG_08162 | XP_003715132.1 |
Canis lupus familiaris (dog) | TUFM | XP_536924.3 |
Bos taurus (cattle) | TUFM | NP_776632.1 |
Rattus norvegicus (Norway rat) | Tufm | NP_001099765.1 |
Danio rerio (zebrafish) | tufm | NP_001013523.1 |
Anopheles gambiae (African malaria mosquito) | AgaP_AGAP006996 | XP_308774.4 |
Neurospora crassa | NCU03737 | XP_961513.1 |
Pan troglodytes (chimpanzee) | TUFM | XP_510904.2 |
Drosophila melanogaster (fruit fly) | EfTuM | NP_001163144.1 |
Schizosaccharomyces pombe (fission yeast) | tuf1 | NP_595746.1 |
Arabidopsis thaliana (thale cress) | AT4G02930 | NP_192202.1 |
Oryza sativa (rice) | Os03g0851100 | NP_001051912.1 |
Mus musculus (house mouse) | Tufm | NP_766333.1 |
Caenorhabditis elegans (roundworm) | tufm-1 | NP_497623.1 |
Analysis of the functional consequences of lethal mutations in mitochondrial translational elongation factors.
Akama K, Christian BE, Jones CN, Ueda T, Takeuchi N, Spremulli LL
Biochimica et biophysica acta1802(7-8)692-8(2010 Jul-Aug)
A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)
Chaperone properties of mammalian mitochondrial translation elongation factor Tu.
Suzuki H, Ueda T, Taguchi H, Takeuchi N
The Journal of biological chemistry282(6)4076-84(2007 Feb)
Crystal structure of the bovine mitochondrial elongation factor Tu.Ts complex.
Jeppesen MG, Navratil T, Spremulli LL, Nyborg J
The Journal of biological chemistry280(6)5071-81(2005 Feb)
Mutagenesis of Arg335 in bovine mitochondrial elongation factor Tu and the corresponding residue in the Escherichia coli factor affects interactions with mitochondrial aminoacyl-tRNAs.
Hunter SE, Spremulli LL
RNA biology1(2)95-102(2004 Jul)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Loss of activity is caused by a significant reduction in the ability of EF-Ts(mt) R325W to bind EF-Tu(mt), leading to a defect in nucleotide exchange.
Title: Analysis of the functional consequences of lethal mutations in mitochondrial translational elongation factors.
Recombinant mitochondrial EF-Tu prevents thermal aggregation of proteins and enhances protein refolding in vitro.
Title: Chaperone properties of mammalian mitochondrial translation elongation factor Tu.
X-ray crystallographic structure of the EF-Tumt.Tsmt complex
Title: Crystal structure of the bovine mitochondrial elongation factor Tu.Ts complex.
The arginine residue at position 335 of bovine mitochondrial elongation factor Tu (EF-Tu) plays an important role in the binding and EF-Tu-mediated delivery of mitochondrial aminoacyl-tRNAs to the A-site of the ribosome.
Title: Mutagenesis of Arg335 in bovine mitochondrial elongation factor Tu and the corresponding residue in the Escherichia coli factor affects interactions with mitochondrial aminoacyl-tRNAs.
Isolation, crystallisation, and preliminary X-ray analysis
Title: Isolation, crystallisation, and preliminary X-ray analysis of the bovine mitochondrial EF-Tu:GDP and EF-Tu:EF-Ts complexes.
The following TUFM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TUFM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OBa41643 | |
Clone ID Related Accession (Same CDS sequence) | NM_174207.2 | |
Accession Version | NM_174207.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-18 | |
Organism | Bos taurus(cattle) | |
Product | elongation factor Tu, mitochondrial precursor | |
Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L38996.1. On Jun 3, 2003 this sequence version replaced NM_174207.1. ##Evidence-Data-START## Transcript exon combination :: L38996.1, BC120109.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## |
1 | ATGGCGGCCG CTACCCTGCT GCGCGCGACG CCCCTTTTCA GCGGTCTCGG CGCCGGCCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_776632.1 |
CDS | 67..1425 |
Translation |
Target ORF information:
Target ORF information:
|
NM_174207.2 |
1 | ATGGCGGCCG CTACCCTGCT GCGCGCGACG CCCCTTTTCA GCGGTCTCGG CGCCGGCCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Analysis of the functional consequences of lethal mutations in mitochondrial translational elongation factors. |
A whole-genome assembly of the domestic cow, Bos taurus. |
Chaperone properties of mammalian mitochondrial translation elongation factor Tu. |
Crystal structure of the bovine mitochondrial elongation factor Tu.Ts complex. |
Mutagenesis of Arg335 in bovine mitochondrial elongation factor Tu and the corresponding residue in the Escherichia coli factor affects interactions with mitochondrial aminoacyl-tRNAs. |