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TUFM cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol TUFM
Entrez Gene ID 7284
Full Name Tu translation elongation factor, mitochondrial
Synonyms COXPD4,EF-TuMT,EFTU,P43
General protein information
Preferred Names

Tu translation elongation factor, mitochondrial

Names

elongation factor Tu, mitochondrial
EF-Tu
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

16

16p11.2
Summary This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq, Jul 2008].
Disorder MIM:

602389

Disorder Html: Combined oxidative phosphorylation deficiency 4, 610678 (3)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_011545928.2 XP_011544230.1 elongation factor Tu, mitochondrial isoform X2
NM_003321.4 NP_003312.3 elongation factor Tu, mitochondrial precursor
XM_017023619.1 XP_016879108.1 elongation factor Tu, mitochondrial isoform X1
NM_001365360.1 NP_001352289.1 elongation factor Tu, mitochondrial isoform 2 precursor
NM_001365360.2 NP_001352289.1 elongation factor Tu, mitochondrial isoform 2 precursor
NM_003321.5 NP_003312.3 elongation factor Tu, mitochondrial isoform 1 precursor

Pathways from BioSystems


Homologies


TUFM XP_001103519.1
Homo sapiens (human) TUFM NP_003312.3
Saccharomyces cerevisiae (baker's yeast) TUF1 NP_014830.1
Kluyveromyces lactis KLLA0A04587g XP_451201.1
Kluyveromyces lactis KLLA0C05214g XP_452433.1
Eremothecium gossypii AGOS_AAR143W NP_982685.1
Magnaporthe oryzae (rice blast fungus) MGG_08162 XP_003715132.1
Canis lupus familiaris (dog) TUFM XP_536924.3
Bos taurus (cattle) TUFM NP_776632.1
Rattus norvegicus (Norway rat) Tufm NP_001099765.1
Danio rerio (zebrafish) tufm NP_001013523.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP006996 XP_308774.4
Neurospora crassa NCU03737 XP_961513.1
Pan troglodytes (chimpanzee) TUFM XP_510904.2
Drosophila melanogaster (fruit fly) EfTuM NP_001163144.1
Schizosaccharomyces pombe (fission yeast) tuf1 NP_595746.1
Arabidopsis thaliana (thale cress) AT4G02930 NP_192202.1
Oryza sativa (rice) Os03g0851100 NP_001051912.1
Mus musculus (house mouse) Tufm NP_766333.1
Caenorhabditis elegans (roundworm) tufm-1 NP_497623.1

Gene Ontology


Bibliography

Related articles in PubMed

Inhibition of Avian Influenza A Virus Replication in Human Cells by Host Restriction Factor TUFM Is Correlated with Autophagy.
Kuo SM, Chen CJ, Chang SC, Liu TJ, Chen YH, Huang SY, Shih SR
mBio8(3)(2017 Jun)

Novel mutation in mitochondrial Elongation Factor EF-Tu associated to dysplastic leukoencephalopathy and defective mitochondrial DNA translation.
Di Nottia M, Montanari A, Verrigni D, Oliva R, Torraco A, Fernandez-Vizarra E, Diodato D, Rizza T, Bianchi M, Catteruccia M, Zeviani M, Dionisi-Vici C, Francisci S, Bertini E, Carrozzo R
Biochimica et biophysica acta1863(4)961-967(2017 Apr)

Expression and clinicopathologic significance of TUFM and p53 for the normal-adenoma-carcinoma sequence in colorectal epithelia.
Xi HQ, Zhang KC, Li JY, Cui JX, Zhao P, Chen L
World journal of surgical oncology15(1)90(2017 Apr)

EGFR-targeted mAb therapy modulates autophagy in head and neck squamous cell carcinoma through NLRX1-TUFM protein complex.
Lei Y, Kansy BA, Li J, Cong L, Liu Y, Trivedi S, Wen H, Ting JP, Ouyang H, Ferris RL
Oncogene35(36)4698-707(2016 Sep)

TUFM downregulation induces epithelial-mesenchymal transition and invasion in lung cancer cells via a mechanism involving AMPK-GSK3? signaling.
He K, Guo X, Liu Y, Li J, Hu Y, Wang D, Song J
Cellular and molecular life sciences : CMLS73(10)2105-21(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High expression of TUFM is associated with colorectal cancer.
Title: Expression and clinicopathologic significance of TUFM and p53 for the normal-adenoma-carcinoma sequence in colorectal epithelia.

we identify a novel signaling hub centering on the NLRX1 TUFM protein complex, promoting autophagic flux. Defects in the expression of either NLRX1 or TUFM result in compromised autophagy when treated with EGFR inhibitors.These findings expand our understanding of the components involved in head and neck squamous cell carcinoma autophagy machinery that responds to EGFR inhibitors.
Title: EGFR-targeted mAb therapy modulates autophagy in head and neck squamous cell carcinoma through NLRX1-TUFM protein complex.

TUFM is a novel regulator of epithelial-mesenchymal transition (EMT); there may be a molecular link between mitochondrial dysfunction and EMT induction
Title: TUFM downregulation induces epithelial-mesenchymal transition and invasion in lung cancer cells via a mechanism involving AMPK-GSK3? signaling.

NLRX1 and TUFM work in concert to reduce cytokine response and augment autophagy.
Title: The NLR protein, NLRX1, and its partner, TUFM, reduce type I interferon, and enhance autophagy.

Increased expression of TUFM is a promising new prognostic indicator for colorectal carcinoma.
Title: TUFM is a potential new prognostic indicator for colorectal carcinoma.

The following TUFM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TUFM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu18903 NM_003321.5
Latest version!		 Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$159.50-$223.30
$319.00
OHu18903 XM_017023619.1
Latest version!		 Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$159.50-$223.30
$319.00
OHu61179 NM_001365360.2
Latest version!		 Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu61179 XM_011545928.2
Latest version!		 Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
View Old Accession Versions >>
OHu61179 NM_001365360.1 Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu18903 NM_003321.4 Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$159.50-$223.30
$319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu18903
Clone ID Related Accession (Same CDS sequence) NM_003321.4 , XM_017023619.1 , NM_003321.5
Accession Version NM_003321.4 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1368bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-11-11
Organism Homo sapiens(human)
Product elongation factor Tu, mitochondrial precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC133550.2, BC010041.2 and CA420396.1. This sequence is a reference standard in the RefSeqGene project. On Mar 7, 2008 this sequence version replaced NM_003321.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.251890.1, SRR1803612.72950.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
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841
901
961
1021
1081
1141
1201
1261
1321
ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGT 
ACACTAGAGC GTGGCATTTT AAAGAAGGGA GACGAGTGTG AGCTCCTAGG ACATAGCAAG 
AACATCCGCA CTGTGGTGAC AGGCATTGAG ATGTTCCACA AGAGCCTGGA GAGGGCCGAG 
GCCGGAGATA ACCTCGGGGC CCTGGTCCGA GGCTTGAAGC GGGAGGACTT GCGGCGGGGC 
CTGGTCATGG TCAAGCCAGG TTCCATCAAG CCCCACCAGA AGGTGGAGGC CCAGGTTTAC 
ATCCTCAGCA AGGAGGAAGG TGGCCGCCAC AAGCCCTTTG TGTCCCACTT CATGCCTGTC 
ATGTTCTCCC TGACTTGGGA CATGGCCTGT CGGATTATCC TGCCCCCAGA GAAGGAGCTT 
GCCATGCCCG GGGAGGACCT GAAGTTCAAC CTAATCTTGC GGCAGCCAAT GATCTTAGAG 
AAAGGCCAGC GTTTCACCCT GCGAGATGGC AACCGGACTA TTGGCACCGG TCTAGTCACC 
AACACGCTGG CCATGACTGA GGAGGAGAAG AATATCAAAT GGGGTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003312.3
CDS140..1507
Translation

Target ORF information:

RefSeq Version NM_003321.4
Organism Homo sapiens(human)
Definition Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_003321.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGT 
ACACTAGAGC GTGGCATTTT AAAGAAGGGA GACGAGTGTG AGCTCCTAGG ACATAGCAAG 
AACATCCGCA CTGTGGTGAC AGGCATTGAG ATGTTCCACA AGAGCCTGGA GAGGGCCGAG 
GCCGGAGATA ACCTCGGGGC CCTGGTCCGA GGCTTGAAGC GGGAGGACTT GCGGCGGGGC 
CTGGTCATGG TCAAGCCAGG TTCCATCAAG CCCCACCAGA AGGTGGAGGC CCAGGTTTAC 
ATCCTCAGCA AGGAGGAAGG TGGCCGCCAC AAGCCCTTTG TGTCCCACTT CATGCCTGTC 
ATGTTCTCCC TGACTTGGGA CATGGCCTGT CGGATTATCC TGCCCCCAGA GAAGGAGCTT 
GCCATGCCCG GGGAGGACCT GAAGTTCAAC CTAATCTTGC GGCAGCCAAT GATCTTAGAG 
AAAGGCCAGC GTTTCACCCT GCGAGATGGC AACCGGACTA TTGGCACCGG TCTAGTCACC 
AACACGCTGG CCATGACTGA GGAGGAGAAG AATATCAAAT GGGGTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18903
Clone ID Related Accession (Same CDS sequence) NM_003321.4 , XM_017023619.1 , NM_003321.5
Accession Version NM_003321.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1368bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-30
Organism Homo sapiens(human)
Product elongation factor Tu, mitochondrial isoform 1 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC133550.2, BC010041.2 and CA420396.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_003321.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.291522.1, SRR1803613.251890.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000313511.8/ ENSP00000322439.3 RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGT 
ACACTAGAGC GTGGCATTTT AAAGAAGGGA GACGAGTGTG AGCTCCTAGG ACATAGCAAG 
AACATCCGCA CTGTGGTGAC AGGCATTGAG ATGTTCCACA AGAGCCTGGA GAGGGCCGAG 
GCCGGAGATA ACCTCGGGGC CCTGGTCCGA GGCTTGAAGC GGGAGGACTT GCGGCGGGGC 
CTGGTCATGG TCAAGCCAGG TTCCATCAAG CCCCACCAGA AGGTGGAGGC CCAGGTTTAC 
ATCCTCAGCA AGGAGGAAGG TGGCCGCCAC AAGCCCTTTG TGTCCCACTT CATGCCTGTC 
ATGTTCTCCC TGACTTGGGA CATGGCCTGT CGGATTATCC TGCCCCCAGA GAAGGAGCTT 
GCCATGCCCG GGGAGGACCT GAAGTTCAAC CTAATCTTGC GGCAGCCAAT GATCTTAGAG 
AAAGGCCAGC GTTTCACCCT GCGAGATGGC AACCGGACTA TTGGCACCGG TCTAGTCACC 
AACACGCTGG CCATGACTGA GGAGGAGAAG AATATCAAAT GGGGTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003312.3
CDS80..1447
Translation

Target ORF information:

RefSeq Version NM_003321.5
Organism Homo sapiens(human)
Definition Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_003321.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGT 
ACACTAGAGC GTGGCATTTT AAAGAAGGGA GACGAGTGTG AGCTCCTAGG ACATAGCAAG 
AACATCCGCA CTGTGGTGAC AGGCATTGAG ATGTTCCACA AGAGCCTGGA GAGGGCCGAG 
GCCGGAGATA ACCTCGGGGC CCTGGTCCGA GGCTTGAAGC GGGAGGACTT GCGGCGGGGC 
CTGGTCATGG TCAAGCCAGG TTCCATCAAG CCCCACCAGA AGGTGGAGGC CCAGGTTTAC 
ATCCTCAGCA AGGAGGAAGG TGGCCGCCAC AAGCCCTTTG TGTCCCACTT CATGCCTGTC 
ATGTTCTCCC TGACTTGGGA CATGGCCTGT CGGATTATCC TGCCCCCAGA GAAGGAGCTT 
GCCATGCCCG GGGAGGACCT GAAGTTCAAC CTAATCTTGC GGCAGCCAAT GATCTTAGAG 
AAAGGCCAGC GTTTCACCCT GCGAGATGGC AACCGGACTA TTGGCACCGG TCTAGTCACC 
AACACGCTGG CCATGACTGA GGAGGAGAAG AATATCAAAT GGGGTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18903
Clone ID Related Accession (Same CDS sequence) NM_003321.4 , XM_017023619.1 , NM_003321.5
Accession Version XM_017023619.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1368bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product elongation factor Tu, mitochondrial isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGT 
ACACTAGAGC GTGGCATTTT AAAGAAGGGA GACGAGTGTG AGCTCCTAGG ACATAGCAAG 
AACATCCGCA CTGTGGTGAC AGGCATTGAG ATGTTCCACA AGAGCCTGGA GAGGGCCGAG 
GCCGGAGATA ACCTCGGGGC CCTGGTCCGA GGCTTGAAGC GGGAGGACTT GCGGCGGGGC 
CTGGTCATGG TCAAGCCAGG TTCCATCAAG CCCCACCAGA AGGTGGAGGC CCAGGTTTAC 
ATCCTCAGCA AGGAGGAAGG TGGCCGCCAC AAGCCCTTTG TGTCCCACTT CATGCCTGTC 
ATGTTCTCCC TGACTTGGGA CATGGCCTGT CGGATTATCC TGCCCCCAGA GAAGGAGCTT 
GCCATGCCCG GGGAGGACCT GAAGTTCAAC CTAATCTTGC GGCAGCCAAT GATCTTAGAG 
AAAGGCCAGC GTTTCACCCT GCGAGATGGC AACCGGACTA TTGGCACCGG TCTAGTCACC 
AACACGCTGG CCATGACTGA GGAGGAGAAG AATATCAAAT GGGGTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016879108.1
CDS397..1764
Translation

Target ORF information:

RefSeq Version XM_017023619.1
Organism Homo sapiens(human)
Definition Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017023619.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGT 
ACACTAGAGC GTGGCATTTT AAAGAAGGGA GACGAGTGTG AGCTCCTAGG ACATAGCAAG 
AACATCCGCA CTGTGGTGAC AGGCATTGAG ATGTTCCACA AGAGCCTGGA GAGGGCCGAG 
GCCGGAGATA ACCTCGGGGC CCTGGTCCGA GGCTTGAAGC GGGAGGACTT GCGGCGGGGC 
CTGGTCATGG TCAAGCCAGG TTCCATCAAG CCCCACCAGA AGGTGGAGGC CCAGGTTTAC 
ATCCTCAGCA AGGAGGAAGG TGGCCGCCAC AAGCCCTTTG TGTCCCACTT CATGCCTGTC 
ATGTTCTCCC TGACTTGGGA CATGGCCTGT CGGATTATCC TGCCCCCAGA GAAGGAGCTT 
GCCATGCCCG GGGAGGACCT GAAGTTCAAC CTAATCTTGC GGCAGCCAAT GATCTTAGAG 
AAAGGCCAGC GTTTCACCCT GCGAGATGGC AACCGGACTA TTGGCACCGG TCTAGTCACC 
AACACGCTGG CCATGACTGA GGAGGAGAAG AATATCAAAT GGGGTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61179
Clone ID Related Accession (Same CDS sequence) XM_011545928.2 , NM_001365360.1 , NM_001365360.2
Accession Version NM_001365360.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-11-24
Organism Homo sapiens(human)
Product elongation factor Tu, mitochondrial isoform 2 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC133550.2. On Aug 20, 2018 this sequence version replaced XM_011545928.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.551915.1, SRR1163657.545971.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGC 
ATTGAGATGT TCCACAAGAG CCTGGAGAGG GCCGAGGCCG GAGATAACCT CGGGGCCCTG 
GTCCGAGGCT TGAAGCGGGA GGACTTGCGG CGGGGCCTGG TCATGGTCAA GCCAGGTTCC 
ATCAAGCCCC ACCAGAAGGT GGAGGCCCAG GTTTACATCC TCAGCAAGGA GGAAGGTGGC 
CGCCACAAGC CCTTTGTGTC CCACTTCATG CCTGTCATGT TCTCCCTGAC TTGGGACATG 
GCCTGTCGGA TTATCCTGCC CCCAGAGAAG GAGCTTGCCA TGCCCGGGGA GGACCTGAAG 
TTCAACCTAA TCTTGCGGCA GCCAATGATC TTAGAGAAAG GCCAGCGTTT CACCCTGCGA 
GATGGCAACC GGACTATTGG CACCGGTCTA GTCACCAACA CGCTGGCCAT GACTGAGGAG 
GAGAAGAATA TCAAATGGGG TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001352289.1
CDS80..1363
Translation

Target ORF information:

RefSeq Version NM_001365360.1
Organism Homo sapiens(human)
Definition Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001365360.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGC 
ATTGAGATGT TCCACAAGAG CCTGGAGAGG GCCGAGGCCG GAGATAACCT CGGGGCCCTG 
GTCCGAGGCT TGAAGCGGGA GGACTTGCGG CGGGGCCTGG TCATGGTCAA GCCAGGTTCC 
ATCAAGCCCC ACCAGAAGGT GGAGGCCCAG GTTTACATCC TCAGCAAGGA GGAAGGTGGC 
CGCCACAAGC CCTTTGTGTC CCACTTCATG CCTGTCATGT TCTCCCTGAC TTGGGACATG 
GCCTGTCGGA TTATCCTGCC CCCAGAGAAG GAGCTTGCCA TGCCCGGGGA GGACCTGAAG 
TTCAACCTAA TCTTGCGGCA GCCAATGATC TTAGAGAAAG GCCAGCGTTT CACCCTGCGA 
GATGGCAACC GGACTATTGG CACCGGTCTA GTCACCAACA CGCTGGCCAT GACTGAGGAG 
GAGAAGAATA TCAAATGGGG TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61179
Clone ID Related Accession (Same CDS sequence) XM_011545928.2 , NM_001365360.1 , NM_001365360.2
Accession Version NM_001365360.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-03
Organism Homo sapiens(human)
Product elongation factor Tu, mitochondrial isoform 2 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC133550.2. On May 31, 2019 this sequence version replaced NM_001365360.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.551915.1, SRR5189667.403990.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGC 
ATTGAGATGT TCCACAAGAG CCTGGAGAGG GCCGAGGCCG GAGATAACCT CGGGGCCCTG 
GTCCGAGGCT TGAAGCGGGA GGACTTGCGG CGGGGCCTGG TCATGGTCAA GCCAGGTTCC 
ATCAAGCCCC ACCAGAAGGT GGAGGCCCAG GTTTACATCC TCAGCAAGGA GGAAGGTGGC 
CGCCACAAGC CCTTTGTGTC CCACTTCATG CCTGTCATGT TCTCCCTGAC TTGGGACATG 
GCCTGTCGGA TTATCCTGCC CCCAGAGAAG GAGCTTGCCA TGCCCGGGGA GGACCTGAAG 
TTCAACCTAA TCTTGCGGCA GCCAATGATC TTAGAGAAAG GCCAGCGTTT CACCCTGCGA 
GATGGCAACC GGACTATTGG CACCGGTCTA GTCACCAACA CGCTGGCCAT GACTGAGGAG 
GAGAAGAATA TCAAATGGGG TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001352289.1
CDS80..1363
Translation

Target ORF information:

RefSeq Version NM_001365360.2
Organism Homo sapiens(human)
Definition Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001365360.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGC 
ATTGAGATGT TCCACAAGAG CCTGGAGAGG GCCGAGGCCG GAGATAACCT CGGGGCCCTG 
GTCCGAGGCT TGAAGCGGGA GGACTTGCGG CGGGGCCTGG TCATGGTCAA GCCAGGTTCC 
ATCAAGCCCC ACCAGAAGGT GGAGGCCCAG GTTTACATCC TCAGCAAGGA GGAAGGTGGC 
CGCCACAAGC CCTTTGTGTC CCACTTCATG CCTGTCATGT TCTCCCTGAC TTGGGACATG 
GCCTGTCGGA TTATCCTGCC CCCAGAGAAG GAGCTTGCCA TGCCCGGGGA GGACCTGAAG 
TTCAACCTAA TCTTGCGGCA GCCAATGATC TTAGAGAAAG GCCAGCGTTT CACCCTGCGA 
GATGGCAACC GGACTATTGG CACCGGTCTA GTCACCAACA CGCTGGCCAT GACTGAGGAG 
GAGAAGAATA TCAAATGGGG TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61179
Clone ID Related Accession (Same CDS sequence) XM_011545928.2 , NM_001365360.1 , NM_001365360.2
Accession Version XM_011545928.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product elongation factor Tu, mitochondrial isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545928.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGC 
ATTGAGATGT TCCACAAGAG CCTGGAGAGG GCCGAGGCCG GAGATAACCT CGGGGCCCTG 
GTCCGAGGCT TGAAGCGGGA GGACTTGCGG CGGGGCCTGG TCATGGTCAA GCCAGGTTCC 
ATCAAGCCCC ACCAGAAGGT GGAGGCCCAG GTTTACATCC TCAGCAAGGA GGAAGGTGGC 
CGCCACAAGC CCTTTGTGTC CCACTTCATG CCTGTCATGT TCTCCCTGAC TTGGGACATG 
GCCTGTCGGA TTATCCTGCC CCCAGAGAAG GAGCTTGCCA TGCCCGGGGA GGACCTGAAG 
TTCAACCTAA TCTTGCGGCA GCCAATGATC TTAGAGAAAG GCCAGCGTTT CACCCTGCGA 
GATGGCAACC GGACTATTGG CACCGGTCTA GTCACCAACA CGCTGGCCAT GACTGAGGAG 
GAGAAGAATA TCAAATGGGG TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011544230.1
CDS234..1517
Translation

Target ORF information:

RefSeq Version XM_011545928.2
Organism Homo sapiens(human)
Definition Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011545928.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGC 
ATTGAGATGT TCCACAAGAG CCTGGAGAGG GCCGAGGCCG GAGATAACCT CGGGGCCCTG 
GTCCGAGGCT TGAAGCGGGA GGACTTGCGG CGGGGCCTGG TCATGGTCAA GCCAGGTTCC 
ATCAAGCCCC ACCAGAAGGT GGAGGCCCAG GTTTACATCC TCAGCAAGGA GGAAGGTGGC 
CGCCACAAGC CCTTTGTGTC CCACTTCATG CCTGTCATGT TCTCCCTGAC TTGGGACATG 
GCCTGTCGGA TTATCCTGCC CCCAGAGAAG GAGCTTGCCA TGCCCGGGGA GGACCTGAAG 
TTCAACCTAA TCTTGCGGCA GCCAATGATC TTAGAGAAAG GCCAGCGTTT CACCCTGCGA 
GATGGCAACC GGACTATTGG CACCGGTCTA GTCACCAACA CGCTGGCCAT GACTGAGGAG 
GAGAAGAATA TCAAATGGGG TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Inhibition of Avian Influenza A Virus Replication in Human Cells by Host Restriction Factor TUFM Is Correlated with Autophagy.
mBio8(3)(2017 Jun)
Kuo SM,Chen CJ,Chang SC,Liu TJ,Chen YH,Huang SY,Shih SR


book

Novel mutation in mitochondrial Elongation Factor EF-Tu associated to dysplastic leukoencephalopathy and defective mitochondrial DNA translation.
Biochimica et biophysica acta1863(4)961-967(2017 Apr)
Di Nottia M,Montanari A,Verrigni D,Oliva R,Torraco A,Fernandez-Vizarra E,Diodato D,Rizza T,Bianchi M,Catteruccia M,Zeviani M,Dionisi-Vici C,Francisci S,Bertini E,Carrozzo R


book

Expression and clinicopathologic significance of TUFM and p53 for the normal-adenoma-carcinoma sequence in colorectal epithelia.
World journal of surgical oncology15(1)90(2017 Apr)
Xi HQ,Zhang KC,Li JY,Cui JX,Zhao P,Chen L


book

EGFR-targeted mAb therapy modulates autophagy in head and neck squamous cell carcinoma through NLRX1-TUFM protein complex.
Oncogene35(36)4698-707(2016 Sep)
Lei Y,Kansy BA,Li J,Cong L,Liu Y,Trivedi S,Wen H,Ting JP,Ouyang H,Ferris RL


book

TUFM downregulation induces epithelial-mesenchymal transition and invasion in lung cancer cells via a mechanism involving AMPK-GSK3? signaling.
Cellular and molecular life sciences : CMLS73(10)2105-21(2016 May)
He K,Guo X,Liu Y,Li J,Hu Y,Wang D,Song J