PRPF8 cDNA ORF clone, Bos taurus(cattle)

  • Gene

  • Clones

  • gRNAs

The following PRPF8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRPF8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa29516 NM_001105638.1
Latest version!
Bos taurus pre-mRNA processing factor 8 (PRPF8), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa29516 XM_005220080.3
Latest version!
Bos taurus pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa29516 XM_024979809.1
Latest version!
Bos taurus pre-mRNA processing factor 8 (PRPF8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
View Old Accession Versions >>
OBa29516 XM_005220080.2 Bos taurus pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa29516
    Clone ID Related Accession (Same CDS sequence) XM_024979809.1 , NM_001105638.1 , XM_005220080.2 , XM_005220080.3
    Accession Version NM_001105638.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product pre-mRNA-processing-splicing factor 8
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC153223.1. On or before Dec 11, 2007 this sequence version replaced XR_027872.1, XR_027883.1. ##Evidence-Data-START## Transcript exon combination :: BC153223.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC TTTAGCCCCG 
    CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CCCGGAAATG GCAACAATTG
    CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC
    ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG
    TTTCGGCATG ATAAAAGGGT GTACTTGGGG GCCCTCAAGT ACATGCCGCA TGCAGTCCTC
    AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC
    CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC
    ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT
    TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC
    AATATTCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT
    GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTAA GGGATAGCCG GAAGTATGTA
    AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC
    CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG
    AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG
    CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGACATCAAC
    AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAC
    AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC
    AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC
    CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTTGAGCTC
    CCAGAATTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT
    GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG
    GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT
    GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTCAA TGCCCTGAAG
    CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA
    TTCTTCCAGT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TACAGGTTTG CCGCCAGGGC
    TACAACATGC TCAACCTTCT CATTCACCGA AAGAACCTCA ACTATCTGCA CCTGGATTAC
    AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGGAAGAA ATCTCGTTTT
    GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT
    CATGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT
    ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG
    CAGATTCGTA TGTGCAAGGA CTTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT
    GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC
    ATGCGTGGAA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT
    GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCACAT
    TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG
    ATCAAGCAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG
    AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT
    CGGTACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAGCGC
    ATCCGCCGCG GGGCCACCGT AGACAAGACT GTGTGCAAAA AGAACCTGGG CCGCCTGACT
    CGGCTCTACC TGAAGGCAGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA CGGTCCTTAC
    ATCACAGCGG AGGAAGCGGT GGCGGTGTAT ACCACTACTG TGCATTGGCT GGAAAGCCGT
    AGATTCTCAC CTATCCCCTT CCCCCCGCTC TCCTACAAGC ATGACACCAA GTTGCTGATC
    TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGATTAAA CCAGTCTCAG
    AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC
    ATCAAGCGAC ACCTCCTTAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC
    CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCAC TGGAGAAGAT CACTGATGCC
    TACCTGGACC AGTACCTGTG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC
    AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AATGGTGCCA AGGCATCAAT
    AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGCGAGTGCA ATGTCATGCT GGAGTCACGA
    TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTAATC
    GTTGACCACA ATATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG
    GACATGAACC ATACAAATTC ATACGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATT
    GTACAGTACT ACGGCCTGGT GATGGACTTG CTTGTGTTGG GGTTGCACCG AGCCAGTGAG
    ATGGCCGGGC CACCTCAGAT GCCAAATGAT TTTCTCAGTT TCCAAGACAT AGCCACCGAG
    GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTCTTCAGG
    TTCACAGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TGACAGAGCA CCCTGACCCC
    AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGTATG
    CGCCTCATGA AGCATGATGT CAACCTAGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT
    CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG
    GACAATCCCA ACCTGCTATT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC
    CGCACCAGCT ACGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG
    ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC
    CACAACCGCG TGCGTCAAAT TCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG
    AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC
    ACCCAGGAGC TCCTGGACTT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG
    ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG
    GAGTTGGGTG GACTTGGCAT GCTTTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC
    AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA
    GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT
    GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC
    AGACGATTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAC
    ACTCTGTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT
    GACTTTAAGC AGTACCAGGT TTTGAAGCAG AATCCTTTCT GGTGGACACA CCAGCGGCAT
    GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACCGACA TGATCCAGGC CCTGGGTGGT
    GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGG
    CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT
    GCCCAGCGAT CAGGATTGAA CCAGATTCCC AATCGTAGGT TCACCCTCTG GTGGTCCCCA
    ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACCGGTATC
    TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC
    TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA
    CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT
    AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC
    CGGCCCTCAT TGCTGGCCGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT
    TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC
    CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT
    GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT
    GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG
    TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCGGA GCCCACTGAG
    CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG
    GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC
    AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG
    ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GACAGTTGGC TAAATGGAAG
    ACAGCTGAGG AGGTGGCTGC CCTAATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG
    ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC
    AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG
    AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCACAGA TGGTTCTCTT TAACCTCTAT
    GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG
    CGCGCCCTGC ACGTAAACAA CGACCGCGCG AAGGTGATCC TGAAGCCGGA CAAGACCACG
    ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACCGACG AGGAGTGGAT CAAGGTGGAG
    GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAATGTGGCA
    TCGCTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCACCATCA
    CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT
    GCAACCCAGA CTCGCACTGT CAATAAGCAT GGTGACGAAA TAATCACCTC TACCACCAGC
    AACTACGAGA CCCAGACTTT CTCCTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCC
    GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT
    GGGTACACCT ATATCCTTCC CAAGAACGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT
    CGGGCCCAAA TTGCAGGTTA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG
    GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT
    GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT
    CAGCCCAACG AGTCTCCTCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG
    GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA
    GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG
    AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG
    ATGCTACTGT CGGATCGCTT CCTTGGCTTC TTTATGGTGC CTGCCCAGTC CTCGTGGAAC
    TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAC
    CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG
    CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001099108.1
    CDS212..7219
    Translation

    Target ORF information:

    RefSeq Version NM_001105638.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pre-mRNA processing factor 8 (PRPF8), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001105638.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC TTTAGCCCCG 
    CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CCCGGAAATG GCAACAATTG
    CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC
    ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG
    TTTCGGCATG ATAAAAGGGT GTACTTGGGG GCCCTCAAGT ACATGCCGCA TGCAGTCCTC
    AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC
    CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC
    ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT
    TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC
    AATATTCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT
    GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTAA GGGATAGCCG GAAGTATGTA
    AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC
    CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG
    AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG
    CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGACATCAAC
    AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAC
    AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC
    AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC
    CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTTGAGCTC
    CCAGAATTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT
    GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG
    GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT
    GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTCAA TGCCCTGAAG
    CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA
    TTCTTCCAGT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TACAGGTTTG CCGCCAGGGC
    TACAACATGC TCAACCTTCT CATTCACCGA AAGAACCTCA ACTATCTGCA CCTGGATTAC
    AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGGAAGAA ATCTCGTTTT
    GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT
    CATGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT
    ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG
    CAGATTCGTA TGTGCAAGGA CTTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT
    GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC
    ATGCGTGGAA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT
    GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCACAT
    TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG
    ATCAAGCAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG
    AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT
    CGGTACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAGCGC
    ATCCGCCGCG GGGCCACCGT AGACAAGACT GTGTGCAAAA AGAACCTGGG CCGCCTGACT
    CGGCTCTACC TGAAGGCAGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA CGGTCCTTAC
    ATCACAGCGG AGGAAGCGGT GGCGGTGTAT ACCACTACTG TGCATTGGCT GGAAAGCCGT
    AGATTCTCAC CTATCCCCTT CCCCCCGCTC TCCTACAAGC ATGACACCAA GTTGCTGATC
    TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGATTAAA CCAGTCTCAG
    AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC
    ATCAAGCGAC ACCTCCTTAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC
    CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCAC TGGAGAAGAT CACTGATGCC
    TACCTGGACC AGTACCTGTG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC
    AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AATGGTGCCA AGGCATCAAT
    AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGCGAGTGCA ATGTCATGCT GGAGTCACGA
    TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTAATC
    GTTGACCACA ATATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG
    GACATGAACC ATACAAATTC ATACGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATT
    GTACAGTACT ACGGCCTGGT GATGGACTTG CTTGTGTTGG GGTTGCACCG AGCCAGTGAG
    ATGGCCGGGC CACCTCAGAT GCCAAATGAT TTTCTCAGTT TCCAAGACAT AGCCACCGAG
    GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTCTTCAGG
    TTCACAGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TGACAGAGCA CCCTGACCCC
    AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGTATG
    CGCCTCATGA AGCATGATGT CAACCTAGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT
    CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG
    GACAATCCCA ACCTGCTATT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC
    CGCACCAGCT ACGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG
    ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC
    CACAACCGCG TGCGTCAAAT TCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG
    AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC
    ACCCAGGAGC TCCTGGACTT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG
    ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG
    GAGTTGGGTG GACTTGGCAT GCTTTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC
    AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA
    GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT
    GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC
    AGACGATTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAC
    ACTCTGTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT
    GACTTTAAGC AGTACCAGGT TTTGAAGCAG AATCCTTTCT GGTGGACACA CCAGCGGCAT
    GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACCGACA TGATCCAGGC CCTGGGTGGT
    GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGG
    CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT
    GCCCAGCGAT CAGGATTGAA CCAGATTCCC AATCGTAGGT TCACCCTCTG GTGGTCCCCA
    ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACCGGTATC
    TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC
    TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA
    CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT
    AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC
    CGGCCCTCAT TGCTGGCCGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT
    TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC
    CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT
    GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT
    GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG
    TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCGGA GCCCACTGAG
    CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG
    GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC
    AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG
    ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GACAGTTGGC TAAATGGAAG
    ACAGCTGAGG AGGTGGCTGC CCTAATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG
    ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC
    AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG
    AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCACAGA TGGTTCTCTT TAACCTCTAT
    GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG
    CGCGCCCTGC ACGTAAACAA CGACCGCGCG AAGGTGATCC TGAAGCCGGA CAAGACCACG
    ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACCGACG AGGAGTGGAT CAAGGTGGAG
    GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAATGTGGCA
    TCGCTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCACCATCA
    CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT
    GCAACCCAGA CTCGCACTGT CAATAAGCAT GGTGACGAAA TAATCACCTC TACCACCAGC
    AACTACGAGA CCCAGACTTT CTCCTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCC
    GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT
    GGGTACACCT ATATCCTTCC CAAGAACGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT
    CGGGCCCAAA TTGCAGGTTA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG
    GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT
    GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT
    CAGCCCAACG AGTCTCCTCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG
    GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA
    GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG
    AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG
    ATGCTACTGT CGGATCGCTT CCTTGGCTTC TTTATGGTGC CTGCCCAGTC CTCGTGGAAC
    TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAC
    CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG
    CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29516
    Clone ID Related Accession (Same CDS sequence) XM_024979809.1 , NM_001105638.1 , XM_005220080.2 , XM_005220080.3
    Accession Version XM_024979809.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pre-mRNA-processing-splicing factor 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC TTTAGCCCCG 
    CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CCCGGAAATG GCAACAATTG
    CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC
    ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG
    TTTCGGCATG ATAAAAGGGT GTACTTGGGG GCCCTCAAGT ACATGCCGCA TGCAGTCCTC
    AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC
    CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC
    ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT
    TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC
    AATATTCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT
    GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTAA GGGATAGCCG GAAGTATGTA
    AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC
    CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG
    AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG
    CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGACATCAAC
    AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAC
    AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC
    AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC
    CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTTGAGCTC
    CCAGAATTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT
    GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG
    GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT
    GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTCAA TGCCCTGAAG
    CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA
    TTCTTCCAGT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TACAGGTTTG CCGCCAGGGC
    TACAACATGC TCAACCTTCT CATTCACCGA AAGAACCTCA ACTATCTGCA CCTGGATTAC
    AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGGAAGAA ATCTCGTTTT
    GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT
    CATGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT
    ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG
    CAGATTCGTA TGTGCAAGGA CTTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT
    GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC
    ATGCGTGGAA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT
    GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCACAT
    TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG
    ATCAAGCAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG
    AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT
    CGGTACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAGCGC
    ATCCGCCGCG GGGCCACCGT AGACAAGACT GTGTGCAAAA AGAACCTGGG CCGCCTGACT
    CGGCTCTACC TGAAGGCAGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA CGGTCCTTAC
    ATCACAGCGG AGGAAGCGGT GGCGGTGTAT ACCACTACTG TGCATTGGCT GGAAAGCCGT
    AGATTCTCAC CTATCCCCTT CCCCCCGCTC TCCTACAAGC ATGACACCAA GTTGCTGATC
    TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGATTAAA CCAGTCTCAG
    AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC
    ATCAAGCGAC ACCTCCTTAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC
    CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCAC TGGAGAAGAT CACTGATGCC
    TACCTGGACC AGTACCTGTG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC
    AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AATGGTGCCA AGGCATCAAT
    AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGCGAGTGCA ATGTCATGCT GGAGTCACGA
    TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTAATC
    GTTGACCACA ATATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG
    GACATGAACC ATACAAATTC ATACGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATT
    GTACAGTACT ACGGCCTGGT GATGGACTTG CTTGTGTTGG GGTTGCACCG AGCCAGTGAG
    ATGGCCGGGC CACCTCAGAT GCCAAATGAT TTTCTCAGTT TCCAAGACAT AGCCACCGAG
    GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTCTTCAGG
    TTCACAGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TGACAGAGCA CCCTGACCCC
    AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGTATG
    CGCCTCATGA AGCATGATGT CAACCTAGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT
    CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG
    GACAATCCCA ACCTGCTATT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC
    CGCACCAGCT ACGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG
    ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC
    CACAACCGCG TGCGTCAAAT TCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG
    AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC
    ACCCAGGAGC TCCTGGACTT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG
    ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG
    GAGTTGGGTG GACTTGGCAT GCTTTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC
    AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA
    GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT
    GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC
    AGACGATTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAC
    ACTCTGTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT
    GACTTTAAGC AGTACCAGGT TTTGAAGCAG AATCCTTTCT GGTGGACACA CCAGCGGCAT
    GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACCGACA TGATCCAGGC CCTGGGTGGT
    GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGG
    CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT
    GCCCAGCGAT CAGGATTGAA CCAGATTCCC AATCGTAGGT TCACCCTCTG GTGGTCCCCA
    ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACCGGTATC
    TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC
    TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA
    CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT
    AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC
    CGGCCCTCAT TGCTGGCCGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT
    TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC
    CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT
    GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT
    GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG
    TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCGGA GCCCACTGAG
    CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG
    GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC
    AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG
    ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GACAGTTGGC TAAATGGAAG
    ACAGCTGAGG AGGTGGCTGC CCTAATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG
    ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC
    AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG
    AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCACAGA TGGTTCTCTT TAACCTCTAT
    GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG
    CGCGCCCTGC ACGTAAACAA CGACCGCGCG AAGGTGATCC TGAAGCCGGA CAAGACCACG
    ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACCGACG AGGAGTGGAT CAAGGTGGAG
    GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAATGTGGCA
    TCGCTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCACCATCA
    CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT
    GCAACCCAGA CTCGCACTGT CAATAAGCAT GGTGACGAAA TAATCACCTC TACCACCAGC
    AACTACGAGA CCCAGACTTT CTCCTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCC
    GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT
    GGGTACACCT ATATCCTTCC CAAGAACGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT
    CGGGCCCAAA TTGCAGGTTA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG
    GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT
    GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT
    CAGCCCAACG AGTCTCCTCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG
    GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA
    GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG
    AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG
    ATGCTACTGT CGGATCGCTT CCTTGGCTTC TTTATGGTGC CTGCCCAGTC CTCGTGGAAC
    TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAC
    CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG
    CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835577.1
    CDS93..7100
    Translation

    Target ORF information:

    RefSeq Version XM_024979809.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pre-mRNA processing factor 8 (PRPF8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979809.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC TTTAGCCCCG 
    CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CCCGGAAATG GCAACAATTG
    CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC
    ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG
    TTTCGGCATG ATAAAAGGGT GTACTTGGGG GCCCTCAAGT ACATGCCGCA TGCAGTCCTC
    AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC
    CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC
    ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT
    TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC
    AATATTCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT
    GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTAA GGGATAGCCG GAAGTATGTA
    AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC
    CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG
    AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG
    CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGACATCAAC
    AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAC
    AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC
    AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC
    CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTTGAGCTC
    CCAGAATTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT
    GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG
    GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT
    GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTCAA TGCCCTGAAG
    CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA
    TTCTTCCAGT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TACAGGTTTG CCGCCAGGGC
    TACAACATGC TCAACCTTCT CATTCACCGA AAGAACCTCA ACTATCTGCA CCTGGATTAC
    AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGGAAGAA ATCTCGTTTT
    GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT
    CATGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT
    ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG
    CAGATTCGTA TGTGCAAGGA CTTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT
    GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC
    ATGCGTGGAA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT
    GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCACAT
    TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG
    ATCAAGCAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG
    AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT
    CGGTACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAGCGC
    ATCCGCCGCG GGGCCACCGT AGACAAGACT GTGTGCAAAA AGAACCTGGG CCGCCTGACT
    CGGCTCTACC TGAAGGCAGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA CGGTCCTTAC
    ATCACAGCGG AGGAAGCGGT GGCGGTGTAT ACCACTACTG TGCATTGGCT GGAAAGCCGT
    AGATTCTCAC CTATCCCCTT CCCCCCGCTC TCCTACAAGC ATGACACCAA GTTGCTGATC
    TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGATTAAA CCAGTCTCAG
    AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC
    ATCAAGCGAC ACCTCCTTAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC
    CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCAC TGGAGAAGAT CACTGATGCC
    TACCTGGACC AGTACCTGTG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC
    AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AATGGTGCCA AGGCATCAAT
    AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGCGAGTGCA ATGTCATGCT GGAGTCACGA
    TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTAATC
    GTTGACCACA ATATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG
    GACATGAACC ATACAAATTC ATACGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATT
    GTACAGTACT ACGGCCTGGT GATGGACTTG CTTGTGTTGG GGTTGCACCG AGCCAGTGAG
    ATGGCCGGGC CACCTCAGAT GCCAAATGAT TTTCTCAGTT TCCAAGACAT AGCCACCGAG
    GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTCTTCAGG
    TTCACAGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TGACAGAGCA CCCTGACCCC
    AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGTATG
    CGCCTCATGA AGCATGATGT CAACCTAGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT
    CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG
    GACAATCCCA ACCTGCTATT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC
    CGCACCAGCT ACGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG
    ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC
    CACAACCGCG TGCGTCAAAT TCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG
    AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC
    ACCCAGGAGC TCCTGGACTT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG
    ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG
    GAGTTGGGTG GACTTGGCAT GCTTTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC
    AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA
    GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT
    GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC
    AGACGATTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAC
    ACTCTGTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT
    GACTTTAAGC AGTACCAGGT TTTGAAGCAG AATCCTTTCT GGTGGACACA CCAGCGGCAT
    GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACCGACA TGATCCAGGC CCTGGGTGGT
    GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGG
    CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT
    GCCCAGCGAT CAGGATTGAA CCAGATTCCC AATCGTAGGT TCACCCTCTG GTGGTCCCCA
    ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACCGGTATC
    TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC
    TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA
    CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT
    AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC
    CGGCCCTCAT TGCTGGCCGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT
    TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC
    CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT
    GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT
    GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG
    TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCGGA GCCCACTGAG
    CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG
    GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC
    AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG
    ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GACAGTTGGC TAAATGGAAG
    ACAGCTGAGG AGGTGGCTGC CCTAATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG
    ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC
    AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG
    AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCACAGA TGGTTCTCTT TAACCTCTAT
    GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG
    CGCGCCCTGC ACGTAAACAA CGACCGCGCG AAGGTGATCC TGAAGCCGGA CAAGACCACG
    ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACCGACG AGGAGTGGAT CAAGGTGGAG
    GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAATGTGGCA
    TCGCTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCACCATCA
    CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT
    GCAACCCAGA CTCGCACTGT CAATAAGCAT GGTGACGAAA TAATCACCTC TACCACCAGC
    AACTACGAGA CCCAGACTTT CTCCTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCC
    GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT
    GGGTACACCT ATATCCTTCC CAAGAACGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT
    CGGGCCCAAA TTGCAGGTTA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG
    GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT
    GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT
    CAGCCCAACG AGTCTCCTCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG
    GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA
    GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG
    AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG
    ATGCTACTGT CGGATCGCTT CCTTGGCTTC TTTATGGTGC CTGCCCAGTC CTCGTGGAAC
    TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAC
    CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG
    CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29516
    Clone ID Related Accession (Same CDS sequence) XM_024979809.1 , NM_001105638.1 , XM_005220080.2 , XM_005220080.3
    Accession Version XM_005220080.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pre-mRNA-processing-splicing factor 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005220080.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC TTTAGCCCCG 
    CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CCCGGAAATG GCAACAATTG
    CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC
    ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG
    TTTCGGCATG ATAAAAGGGT GTACTTGGGG GCCCTCAAGT ACATGCCGCA TGCAGTCCTC
    AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC
    CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC
    ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT
    TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC
    AATATTCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT
    GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTAA GGGATAGCCG GAAGTATGTA
    AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC
    CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG
    AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG
    CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGACATCAAC
    AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAC
    AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC
    AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC
    CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTTGAGCTC
    CCAGAATTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT
    GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG
    GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT
    GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTCAA TGCCCTGAAG
    CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA
    TTCTTCCAGT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TACAGGTTTG CCGCCAGGGC
    TACAACATGC TCAACCTTCT CATTCACCGA AAGAACCTCA ACTATCTGCA CCTGGATTAC
    AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGGAAGAA ATCTCGTTTT
    GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT
    CATGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT
    ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG
    CAGATTCGTA TGTGCAAGGA CTTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT
    GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC
    ATGCGTGGAA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT
    GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCACAT
    TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG
    ATCAAGCAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG
    AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT
    CGGTACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAGCGC
    ATCCGCCGCG GGGCCACCGT AGACAAGACT GTGTGCAAAA AGAACCTGGG CCGCCTGACT
    CGGCTCTACC TGAAGGCAGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA CGGTCCTTAC
    ATCACAGCGG AGGAAGCGGT GGCGGTGTAT ACCACTACTG TGCATTGGCT GGAAAGCCGT
    AGATTCTCAC CTATCCCCTT CCCCCCGCTC TCCTACAAGC ATGACACCAA GTTGCTGATC
    TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGATTAAA CCAGTCTCAG
    AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC
    ATCAAGCGAC ACCTCCTTAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC
    CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCAC TGGAGAAGAT CACTGATGCC
    TACCTGGACC AGTACCTGTG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC
    AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AATGGTGCCA AGGCATCAAT
    AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGCGAGTGCA ATGTCATGCT GGAGTCACGA
    TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTAATC
    GTTGACCACA ATATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG
    GACATGAACC ATACAAATTC ATACGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATT
    GTACAGTACT ACGGCCTGGT GATGGACTTG CTTGTGTTGG GGTTGCACCG AGCCAGTGAG
    ATGGCCGGGC CACCTCAGAT GCCAAATGAT TTTCTCAGTT TCCAAGACAT AGCCACCGAG
    GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTCTTCAGG
    TTCACAGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TGACAGAGCA CCCTGACCCC
    AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGTATG
    CGCCTCATGA AGCATGATGT CAACCTAGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT
    CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG
    GACAATCCCA ACCTGCTATT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC
    CGCACCAGCT ACGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG
    ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC
    CACAACCGCG TGCGTCAAAT TCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG
    AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC
    ACCCAGGAGC TCCTGGACTT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG
    ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG
    GAGTTGGGTG GACTTGGCAT GCTTTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC
    AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA
    GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT
    GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC
    AGACGATTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAC
    ACTCTGTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT
    GACTTTAAGC AGTACCAGGT TTTGAAGCAG AATCCTTTCT GGTGGACACA CCAGCGGCAT
    GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACCGACA TGATCCAGGC CCTGGGTGGT
    GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGG
    CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT
    GCCCAGCGAT CAGGATTGAA CCAGATTCCC AATCGTAGGT TCACCCTCTG GTGGTCCCCA
    ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACCGGTATC
    TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC
    TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA
    CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT
    AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC
    CGGCCCTCAT TGCTGGCCGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT
    TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC
    CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT
    GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT
    GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG
    TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCGGA GCCCACTGAG
    CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG
    GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC
    AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG
    ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GACAGTTGGC TAAATGGAAG
    ACAGCTGAGG AGGTGGCTGC CCTAATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG
    ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC
    AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG
    AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCACAGA TGGTTCTCTT TAACCTCTAT
    GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG
    CGCGCCCTGC ACGTAAACAA CGACCGCGCG AAGGTGATCC TGAAGCCGGA CAAGACCACG
    ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACCGACG AGGAGTGGAT CAAGGTGGAG
    GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAATGTGGCA
    TCGCTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCACCATCA
    CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT
    GCAACCCAGA CTCGCACTGT CAATAAGCAT GGTGACGAAA TAATCACCTC TACCACCAGC
    AACTACGAGA CCCAGACTTT CTCCTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCC
    GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT
    GGGTACACCT ATATCCTTCC CAAGAACGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT
    CGGGCCCAAA TTGCAGGTTA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG
    GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT
    GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT
    CAGCCCAACG AGTCTCCTCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG
    GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA
    GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG
    AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG
    ATGCTACTGT CGGATCGCTT CCTTGGCTTC TTTATGGTGC CTGCCCAGTC CTCGTGGAAC
    TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAC
    CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG
    CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220137.1
    CDS118..7125
    Translation

    Target ORF information:

    RefSeq Version XM_005220080.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220080.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC TTTAGCCCCG 
    CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CCCGGAAATG GCAACAATTG
    CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC
    ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG
    TTTCGGCATG ATAAAAGGGT GTACTTGGGG GCCCTCAAGT ACATGCCGCA TGCAGTCCTC
    AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC
    CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC
    ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT
    TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC
    AATATTCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT
    GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTAA GGGATAGCCG GAAGTATGTA
    AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC
    CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG
    AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG
    CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGACATCAAC
    AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAC
    AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC
    AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC
    CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTTGAGCTC
    CCAGAATTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT
    GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG
    GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT
    GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTCAA TGCCCTGAAG
    CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA
    TTCTTCCAGT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TACAGGTTTG CCGCCAGGGC
    TACAACATGC TCAACCTTCT CATTCACCGA AAGAACCTCA ACTATCTGCA CCTGGATTAC
    AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGGAAGAA ATCTCGTTTT
    GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT
    CATGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT
    ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG
    CAGATTCGTA TGTGCAAGGA CTTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT
    GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC
    ATGCGTGGAA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT
    GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCACAT
    TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG
    ATCAAGCAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG
    AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT
    CGGTACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAGCGC
    ATCCGCCGCG GGGCCACCGT AGACAAGACT GTGTGCAAAA AGAACCTGGG CCGCCTGACT
    CGGCTCTACC TGAAGGCAGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA CGGTCCTTAC
    ATCACAGCGG AGGAAGCGGT GGCGGTGTAT ACCACTACTG TGCATTGGCT GGAAAGCCGT
    AGATTCTCAC CTATCCCCTT CCCCCCGCTC TCCTACAAGC ATGACACCAA GTTGCTGATC
    TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGATTAAA CCAGTCTCAG
    AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC
    ATCAAGCGAC ACCTCCTTAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC
    CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCAC TGGAGAAGAT CACTGATGCC
    TACCTGGACC AGTACCTGTG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC
    AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AATGGTGCCA AGGCATCAAT
    AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGCGAGTGCA ATGTCATGCT GGAGTCACGA
    TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTAATC
    GTTGACCACA ATATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG
    GACATGAACC ATACAAATTC ATACGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATT
    GTACAGTACT ACGGCCTGGT GATGGACTTG CTTGTGTTGG GGTTGCACCG AGCCAGTGAG
    ATGGCCGGGC CACCTCAGAT GCCAAATGAT TTTCTCAGTT TCCAAGACAT AGCCACCGAG
    GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTCTTCAGG
    TTCACAGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TGACAGAGCA CCCTGACCCC
    AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGTATG
    CGCCTCATGA AGCATGATGT CAACCTAGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT
    CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG
    GACAATCCCA ACCTGCTATT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC
    CGCACCAGCT ACGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG
    ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC
    CACAACCGCG TGCGTCAAAT TCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG
    AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC
    ACCCAGGAGC TCCTGGACTT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG
    ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG
    GAGTTGGGTG GACTTGGCAT GCTTTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC
    AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA
    GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT
    GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC
    AGACGATTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAC
    ACTCTGTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT
    GACTTTAAGC AGTACCAGGT TTTGAAGCAG AATCCTTTCT GGTGGACACA CCAGCGGCAT
    GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACCGACA TGATCCAGGC CCTGGGTGGT
    GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGG
    CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT
    GCCCAGCGAT CAGGATTGAA CCAGATTCCC AATCGTAGGT TCACCCTCTG GTGGTCCCCA
    ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACCGGTATC
    TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC
    TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA
    CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT
    AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC
    CGGCCCTCAT TGCTGGCCGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT
    TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC
    CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT
    GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT
    GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG
    TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCGGA GCCCACTGAG
    CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG
    GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC
    AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG
    ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GACAGTTGGC TAAATGGAAG
    ACAGCTGAGG AGGTGGCTGC CCTAATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG
    ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC
    AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG
    AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCACAGA TGGTTCTCTT TAACCTCTAT
    GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG
    CGCGCCCTGC ACGTAAACAA CGACCGCGCG AAGGTGATCC TGAAGCCGGA CAAGACCACG
    ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACCGACG AGGAGTGGAT CAAGGTGGAG
    GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAATGTGGCA
    TCGCTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCACCATCA
    CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT
    GCAACCCAGA CTCGCACTGT CAATAAGCAT GGTGACGAAA TAATCACCTC TACCACCAGC
    AACTACGAGA CCCAGACTTT CTCCTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCC
    GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT
    GGGTACACCT ATATCCTTCC CAAGAACGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT
    CGGGCCCAAA TTGCAGGTTA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG
    GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT
    GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT
    CAGCCCAACG AGTCTCCTCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG
    GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA
    GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG
    AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG
    ATGCTACTGT CGGATCGCTT CCTTGGCTTC TTTATGGTGC CTGCCCAGTC CTCGTGGAAC
    TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAC
    CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG
    CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29516
    Clone ID Related Accession (Same CDS sequence) XM_024979809.1 , NM_001105638.1 , XM_005220080.2 , XM_005220080.3
    Accession Version XM_005220080.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pre-mRNA-processing-splicing factor 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220080.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    121
    181
    241
    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC TTTAGCCCCG 
    CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CCCGGAAATG GCAACAATTG
    CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC
    ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG
    TTTCGGCATG ATAAAAGGGT GTACTTGGGG GCCCTCAAGT ACATGCCGCA TGCAGTCCTC
    AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC
    CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC
    ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT
    TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC
    AATATTCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT
    GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTAA GGGATAGCCG GAAGTATGTA
    AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC
    CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG
    AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG
    CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGACATCAAC
    AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAC
    AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC
    AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC
    CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTTGAGCTC
    CCAGAATTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT
    GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG
    GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT
    GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTCAA TGCCCTGAAG
    CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA
    TTCTTCCAGT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TACAGGTTTG CCGCCAGGGC
    TACAACATGC TCAACCTTCT CATTCACCGA AAGAACCTCA ACTATCTGCA CCTGGATTAC
    AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGGAAGAA ATCTCGTTTT
    GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT
    CATGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT
    ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG
    CAGATTCGTA TGTGCAAGGA CTTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT
    GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC
    ATGCGTGGAA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT
    GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCACAT
    TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG
    ATCAAGCAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG
    AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT
    CGGTACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAGCGC
    ATCCGCCGCG GGGCCACCGT AGACAAGACT GTGTGCAAAA AGAACCTGGG CCGCCTGACT
    CGGCTCTACC TGAAGGCAGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA CGGTCCTTAC
    ATCACAGCGG AGGAAGCGGT GGCGGTGTAT ACCACTACTG TGCATTGGCT GGAAAGCCGT
    AGATTCTCAC CTATCCCCTT CCCCCCGCTC TCCTACAAGC ATGACACCAA GTTGCTGATC
    TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGATTAAA CCAGTCTCAG
    AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC
    ATCAAGCGAC ACCTCCTTAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC
    CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCAC TGGAGAAGAT CACTGATGCC
    TACCTGGACC AGTACCTGTG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC
    AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AATGGTGCCA AGGCATCAAT
    AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGCGAGTGCA ATGTCATGCT GGAGTCACGA
    TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTAATC
    GTTGACCACA ATATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG
    GACATGAACC ATACAAATTC ATACGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATT
    GTACAGTACT ACGGCCTGGT GATGGACTTG CTTGTGTTGG GGTTGCACCG AGCCAGTGAG
    ATGGCCGGGC CACCTCAGAT GCCAAATGAT TTTCTCAGTT TCCAAGACAT AGCCACCGAG
    GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTCTTCAGG
    TTCACAGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TGACAGAGCA CCCTGACCCC
    AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGTATG
    CGCCTCATGA AGCATGATGT CAACCTAGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT
    CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG
    GACAATCCCA ACCTGCTATT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC
    CGCACCAGCT ACGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG
    ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC
    CACAACCGCG TGCGTCAAAT TCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG
    AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC
    ACCCAGGAGC TCCTGGACTT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG
    ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG
    GAGTTGGGTG GACTTGGCAT GCTTTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC
    AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA
    GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT
    GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC
    AGACGATTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAC
    ACTCTGTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT
    GACTTTAAGC AGTACCAGGT TTTGAAGCAG AATCCTTTCT GGTGGACACA CCAGCGGCAT
    GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACCGACA TGATCCAGGC CCTGGGTGGT
    GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGG
    CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT
    GCCCAGCGAT CAGGATTGAA CCAGATTCCC AATCGTAGGT TCACCCTCTG GTGGTCCCCA
    ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACCGGTATC
    TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC
    TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA
    CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT
    AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC
    CGGCCCTCAT TGCTGGCCGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT
    TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC
    CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT
    GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT
    GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG
    TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCGGA GCCCACTGAG
    CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG
    GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC
    AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG
    ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GACAGTTGGC TAAATGGAAG
    ACAGCTGAGG AGGTGGCTGC CCTAATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG
    ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC
    AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG
    AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCACAGA TGGTTCTCTT TAACCTCTAT
    GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG
    CGCGCCCTGC ACGTAAACAA CGACCGCGCG AAGGTGATCC TGAAGCCGGA CAAGACCACG
    ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACCGACG AGGAGTGGAT CAAGGTGGAG
    GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAATGTGGCA
    TCGCTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCACCATCA
    CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT
    GCAACCCAGA CTCGCACTGT CAATAAGCAT GGTGACGAAA TAATCACCTC TACCACCAGC
    AACTACGAGA CCCAGACTTT CTCCTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCC
    GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT
    GGGTACACCT ATATCCTTCC CAAGAACGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT
    CGGGCCCAAA TTGCAGGTTA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG
    GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT
    GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT
    CAGCCCAACG AGTCTCCTCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG
    GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA
    GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG
    AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG
    ATGCTACTGT CGGATCGCTT CCTTGGCTTC TTTATGGTGC CTGCCCAGTC CTCGTGGAAC
    TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAC
    CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG
    CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220137.1
    CDS169..7176
    Translation

    Target ORF information:

    RefSeq Version XM_005220080.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220080.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC TTTAGCCCCG 
    CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CCCGGAAATG GCAACAATTG
    CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC
    ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG
    TTTCGGCATG ATAAAAGGGT GTACTTGGGG GCCCTCAAGT ACATGCCGCA TGCAGTCCTC
    AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC
    CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC
    ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT
    TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC
    AATATTCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT
    GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTAA GGGATAGCCG GAAGTATGTA
    AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC
    CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG
    AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG
    CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGACATCAAC
    AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAC
    AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC
    AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC
    CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTTGAGCTC
    CCAGAATTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT
    GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG
    GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT
    GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTCAA TGCCCTGAAG
    CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA
    TTCTTCCAGT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TACAGGTTTG CCGCCAGGGC
    TACAACATGC TCAACCTTCT CATTCACCGA AAGAACCTCA ACTATCTGCA CCTGGATTAC
    AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGGAAGAA ATCTCGTTTT
    GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT
    CATGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT
    ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG
    CAGATTCGTA TGTGCAAGGA CTTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT
    GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC
    ATGCGTGGAA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT
    GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCACAT
    TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG
    ATCAAGCAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG
    AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT
    CGGTACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAGCGC
    ATCCGCCGCG GGGCCACCGT AGACAAGACT GTGTGCAAAA AGAACCTGGG CCGCCTGACT
    CGGCTCTACC TGAAGGCAGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA CGGTCCTTAC
    ATCACAGCGG AGGAAGCGGT GGCGGTGTAT ACCACTACTG TGCATTGGCT GGAAAGCCGT
    AGATTCTCAC CTATCCCCTT CCCCCCGCTC TCCTACAAGC ATGACACCAA GTTGCTGATC
    TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGATTAAA CCAGTCTCAG
    AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC
    ATCAAGCGAC ACCTCCTTAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC
    CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCAC TGGAGAAGAT CACTGATGCC
    TACCTGGACC AGTACCTGTG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC
    AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AATGGTGCCA AGGCATCAAT
    AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGCGAGTGCA ATGTCATGCT GGAGTCACGA
    TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTAATC
    GTTGACCACA ATATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG
    GACATGAACC ATACAAATTC ATACGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATT
    GTACAGTACT ACGGCCTGGT GATGGACTTG CTTGTGTTGG GGTTGCACCG AGCCAGTGAG
    ATGGCCGGGC CACCTCAGAT GCCAAATGAT TTTCTCAGTT TCCAAGACAT AGCCACCGAG
    GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTCTTCAGG
    TTCACAGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TGACAGAGCA CCCTGACCCC
    AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGTATG
    CGCCTCATGA AGCATGATGT CAACCTAGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT
    CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG
    GACAATCCCA ACCTGCTATT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC
    CGCACCAGCT ACGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG
    ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC
    CACAACCGCG TGCGTCAAAT TCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG
    AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC
    ACCCAGGAGC TCCTGGACTT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG
    ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG
    GAGTTGGGTG GACTTGGCAT GCTTTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC
    AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA
    GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT
    GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC
    AGACGATTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAC
    ACTCTGTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT
    GACTTTAAGC AGTACCAGGT TTTGAAGCAG AATCCTTTCT GGTGGACACA CCAGCGGCAT
    GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACCGACA TGATCCAGGC CCTGGGTGGT
    GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGG
    CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT
    GCCCAGCGAT CAGGATTGAA CCAGATTCCC AATCGTAGGT TCACCCTCTG GTGGTCCCCA
    ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACCGGTATC
    TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC
    TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA
    CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT
    AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC
    CGGCCCTCAT TGCTGGCCGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT
    TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC
    CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT
    GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT
    GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG
    TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCGGA GCCCACTGAG
    CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG
    GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC
    AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG
    ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GACAGTTGGC TAAATGGAAG
    ACAGCTGAGG AGGTGGCTGC CCTAATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG
    ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC
    AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG
    AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCACAGA TGGTTCTCTT TAACCTCTAT
    GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG
    CGCGCCCTGC ACGTAAACAA CGACCGCGCG AAGGTGATCC TGAAGCCGGA CAAGACCACG
    ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACCGACG AGGAGTGGAT CAAGGTGGAG
    GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAATGTGGCA
    TCGCTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCACCATCA
    CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT
    GCAACCCAGA CTCGCACTGT CAATAAGCAT GGTGACGAAA TAATCACCTC TACCACCAGC
    AACTACGAGA CCCAGACTTT CTCCTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCC
    GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT
    GGGTACACCT ATATCCTTCC CAAGAACGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT
    CGGGCCCAAA TTGCAGGTTA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG
    GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT
    GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT
    CAGCCCAACG AGTCTCCTCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG
    GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA
    GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG
    AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG
    ATGCTACTGT CGGATCGCTT CCTTGGCTTC TTTATGGTGC CTGCCCAGTC CTCGTGGAAC
    TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAC
    CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG
    CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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