BUB1B cDNA ORF clone, Bos taurus(cattle)

The following BUB1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUB1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa219650 XM_024997695.1
Latest version!
Bos taurus BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06476 NM_001145173.1
Latest version!
Bos taurus BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa219650
    Clone ID Related Accession (Same CDS sequence) XM_024997695.1
    Accession Version XM_024997695.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3204bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGCGA TGCAGAAGGA AAGGGGTGCT CCGAGTGACA TCATGTCCTT GGAGGGAGAT 
    GAATGGGAGC TGAGTAAAGA AAATGTCCAA CCTTTAAGGC AGGGGCGGAT CATGTCCACA
    CTTCAGGGAG CTCTGGCACA GCAAGAATCT GCCTGTAACA CTACTCTTCA ACAACAAAAA
    CGAGCATTTG AATCTGAAAT TCGATTTTAT GCTGGAAATG ACCCTCTGGA TGTTTGGGAT
    AGATATATTA ACTGGACAGA GCAAAACTAT CCTCAGGGCG GGAAGGAAAG CAATATGTCA
    ACATTACTAG AAAGAGCTGT AGAAGCACTA CAAGGAGAAA AACGATATTA TAATGATCCT
    CGATTTCTTA GTCTCTGGCT TAAGCTGGGG CATTTATGCA ATGAACCTTT GGATATGTAC
    AGTTATTTAC ATAACCAAGG AATTGGCATT TCACTTGCTC AGTTTTATAT CTCATGGGCT
    GAAGAATATG AAGCTAGAGA AAACTTTAAG AAAGCAGATC TGATATTTCA AGAAGGTATT
    GAACAGAAGG CTGAACCACT GGAAAGACTG CAGTCCCAGC ACAGACAATT CCAAGCTCGA
    GTGTCTCGGC AAACTCTGTT GGCACTTGAG AAAGAAGAGG AGGAGGAAGT TGTGGGATCT
    TCTGTACCAC AGCGAAGCAC ACTGGCTGAA CTAAAAAGCA AAGGGAAAAA GACAGCAAGA
    GCTCCAATCA GCCGTGTAGG GGGTGCTCTC AAGGCTCCAA GCCAGAACAG AGGTCTCCAA
    AATCCAGTTC CTCAACAGAT GCAAAGTAAT ACTAGAATTA TTGTTTTTGA TGAAAATACT
    GATGAGGCTT CTAGAGCAGA GCCATCTAAG CCTACAGTCC AGCCATGGAT AGCACCTCCT
    GTGTCCAGGG GCAAAGAGAA TGAGCTGCAA GCCGGCCCTT GGAACACAGG CAGGCCTTTG
    GAATACAGGC CTCGAGGCAA TACAGCGTCT ATGACAGCTG TACCCTCTAT GCTTCCAAGT
    TTTACTCCAT ATGTGGAAGA GACTGCACAA CAGCCAGTTA TGACACCATG TAAAATTGAA
    CCCAGTATAA ACCACATTCT AAGCACAAGA AAGCCTGGAA AGGAAGAAGG AGATCTCCTG
    CAGAGAGTTC AAAGTCTTCA GCAAGAGTCT GAGAAGAAGG AGAAGATGAT GTATTGTAAG
    GAGAAGATTT ATGCAGGAGT GGGGGAGTTC TCCTTTGAAG AGATCCGGGC TGAGGTTTTC
    CGGAAGAAAT TAAAAGAGCG AAGAGAAGCT GAGCTACTGA CCAGTGCAGA GAAAAGAGCA
    GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA AACTACTCAG
    CAAGAAAGAG CGAATGAACA GCAAGAAGAA AAGATGCCTA CAAAGGAGAC AGCTGAACTG
    CCAATTGCTT CAGAGTCTCA GGAAATATCA GGAATGGCTC TGTCCAATTT TGTTTGCCCA
    GTAAACTCTT GTGCCAGGGA AACATCACTT GCAGAAAACG TTTGGCAAGA ACAACCTCAT
    TCTAAAATCT CTCTTTCTAT AGGTTCCAGT GTACCTTTCT CCATTTTTGA TGAATCTCTT
    CTTTCAGAAA AAAAGCCTGT CAGTCCTTCT GCAGCTCCCT CACGTGTTTT AGCCCAACGA
    AGACCTCTTG CAATTCTCAA AACTTCAGAA AGCATCACCT CAAATGAGGA TGTATCTCCA
    GATGTTTGTG ATGAATTTAC AGGAATTGAG CCCTTGAGTG AGGATGCTAT TATCACAGGT
    TTCAGGAATG TAACTATTTG TCCTAACCCA GAAGACACTT GTGACTTCGC TAGAGCAGCT
    CGTTTTGCAT CCACTCCTTT TCATGAAATA ATATCCTTGA AGGATCTCCC TTCTGATCCT
    GAGAGACTGT TGCAGGAAGA GGATCTAGAT ATGAAGACCT CTGAGGACCA GCAAACTGCT
    TGTGGCACTA TCTACAATCC ACCTCTCAGC ATCAAGAAGC TGAGCCCAAT TATTGAAGAC
    AGCCGTGAAG CCACACATTC CTCTGGGTTC TCCGGATCTT CTGCCTCAGT TACAAGCACC
    TCCTCCATCA AAAGTCTTCA AATTCCTGAG AAACTGGAGC TCACTAGTGA TACTGCAGAC
    AGCCCTACTC AGTCACCCTG GTGTCCACAG TATCGCAGAC AACTGGTAAA ATCCCTACCA
    GAGCTGAGTG TCTCTACAGA GTTTTTTGTA GAAGATAGAC CAATGCCTAG TTTGGAAATA
    GAGAAGGAAA TTGAATTAGA TAATGAGGAA TATTGCATTA GACAAGAATA CCTGGTATGT
    GAAGACTACA AATTATTCTG GGTGACACCA AGAAACACTG CCGAGTTAAC CATAATAAAG
    GTATCTTCTC ATCCTGTCCC ATGGGACTTC TATATCAACT TCAAGTTAAA GGAACGTTTA
    AATGAGGAGT GTGATAATTT GTGTAGCTGT TATCAATACC AAGATGGCTG TATCGTTTGG
    CATCAATATA TAAACTGCTT CACCCTTCAG GATCTTCTTC AACACAGTGA ATTTATTACC
    CATGAAATGA CGGTATTGAT TATTTATAAC CTTTTGACAA TGGTGGAGAA ACTACACAAA
    GCAGAAATAG TCCATGGTGA CTTGAGTCCA AGGAGCCTGA TTCTTAGAAA CAGAATCCAC
    GATCCCTATG ATTTTAATAA GAACAATCAA GCTTTGAAGA TAGTGGACTT TTCCTACAGT
    GTTGACCTTA GGGCACAGCT AGACGTTTTT AACCTCAGTG GCTTTCGGAC TGTACAAACC
    CTGGAAGGAC AAAAGATCCT GGCTAACTGT TCTTCTCCAT ACCAGGTAGA TCTGTTTGGT
    ATAGCAGATT TAGCACATTT ATTATTGTTC AAGGAGCACC TACAGGTCTT CTGGGATGGT
    TCCCTCTGGA AACTTAGCCA AAATATTTCT GAGCTAAAAG ATGGTGAATT GTGGAATAAA
    TTCTTTTTGC GGATTCTGAA TGCCAGTGAT GAGTCCACAG TGTCTGTTCT GGGGGAACTT
    GCAGCAGAAA TGAATGGGGT GTTTGACACC ACATTCCAAA GTCACCTGAA CAAAGCTTTT
    TGGAAAGTGG GGAAGTTAAT CAGTCCTGGG GCTTTCCTCT TCCAGCAAGA TAGGCAGTCA
    AGCCTCTCAC AGATTCCTGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853463.1
    CDS242..3445
    Translation

    Target ORF information:

    RefSeq Version XM_024997695.1
    Organism Bos taurus(cattle)
    Definition Bos taurus BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997695.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGCGA TGCAGAAGGA AAGGGGTGCT CCGAGTGACA TCATGTCCTT GGAGGGAGAT 
    GAATGGGAGC TGAGTAAAGA AAATGTCCAA CCTTTAAGGC AGGGGCGGAT CATGTCCACA
    CTTCAGGGAG CTCTGGCACA GCAAGAATCT GCCTGTAACA CTACTCTTCA ACAACAAAAA
    CGAGCATTTG AATCTGAAAT TCGATTTTAT GCTGGAAATG ACCCTCTGGA TGTTTGGGAT
    AGATATATTA ACTGGACAGA GCAAAACTAT CCTCAGGGCG GGAAGGAAAG CAATATGTCA
    ACATTACTAG AAAGAGCTGT AGAAGCACTA CAAGGAGAAA AACGATATTA TAATGATCCT
    CGATTTCTTA GTCTCTGGCT TAAGCTGGGG CATTTATGCA ATGAACCTTT GGATATGTAC
    AGTTATTTAC ATAACCAAGG AATTGGCATT TCACTTGCTC AGTTTTATAT CTCATGGGCT
    GAAGAATATG AAGCTAGAGA AAACTTTAAG AAAGCAGATC TGATATTTCA AGAAGGTATT
    GAACAGAAGG CTGAACCACT GGAAAGACTG CAGTCCCAGC ACAGACAATT CCAAGCTCGA
    GTGTCTCGGC AAACTCTGTT GGCACTTGAG AAAGAAGAGG AGGAGGAAGT TGTGGGATCT
    TCTGTACCAC AGCGAAGCAC ACTGGCTGAA CTAAAAAGCA AAGGGAAAAA GACAGCAAGA
    GCTCCAATCA GCCGTGTAGG GGGTGCTCTC AAGGCTCCAA GCCAGAACAG AGGTCTCCAA
    AATCCAGTTC CTCAACAGAT GCAAAGTAAT ACTAGAATTA TTGTTTTTGA TGAAAATACT
    GATGAGGCTT CTAGAGCAGA GCCATCTAAG CCTACAGTCC AGCCATGGAT AGCACCTCCT
    GTGTCCAGGG GCAAAGAGAA TGAGCTGCAA GCCGGCCCTT GGAACACAGG CAGGCCTTTG
    GAATACAGGC CTCGAGGCAA TACAGCGTCT ATGACAGCTG TACCCTCTAT GCTTCCAAGT
    TTTACTCCAT ATGTGGAAGA GACTGCACAA CAGCCAGTTA TGACACCATG TAAAATTGAA
    CCCAGTATAA ACCACATTCT AAGCACAAGA AAGCCTGGAA AGGAAGAAGG AGATCTCCTG
    CAGAGAGTTC AAAGTCTTCA GCAAGAGTCT GAGAAGAAGG AGAAGATGAT GTATTGTAAG
    GAGAAGATTT ATGCAGGAGT GGGGGAGTTC TCCTTTGAAG AGATCCGGGC TGAGGTTTTC
    CGGAAGAAAT TAAAAGAGCG AAGAGAAGCT GAGCTACTGA CCAGTGCAGA GAAAAGAGCA
    GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA AACTACTCAG
    CAAGAAAGAG CGAATGAACA GCAAGAAGAA AAGATGCCTA CAAAGGAGAC AGCTGAACTG
    CCAATTGCTT CAGAGTCTCA GGAAATATCA GGAATGGCTC TGTCCAATTT TGTTTGCCCA
    GTAAACTCTT GTGCCAGGGA AACATCACTT GCAGAAAACG TTTGGCAAGA ACAACCTCAT
    TCTAAAATCT CTCTTTCTAT AGGTTCCAGT GTACCTTTCT CCATTTTTGA TGAATCTCTT
    CTTTCAGAAA AAAAGCCTGT CAGTCCTTCT GCAGCTCCCT CACGTGTTTT AGCCCAACGA
    AGACCTCTTG CAATTCTCAA AACTTCAGAA AGCATCACCT CAAATGAGGA TGTATCTCCA
    GATGTTTGTG ATGAATTTAC AGGAATTGAG CCCTTGAGTG AGGATGCTAT TATCACAGGT
    TTCAGGAATG TAACTATTTG TCCTAACCCA GAAGACACTT GTGACTTCGC TAGAGCAGCT
    CGTTTTGCAT CCACTCCTTT TCATGAAATA ATATCCTTGA AGGATCTCCC TTCTGATCCT
    GAGAGACTGT TGCAGGAAGA GGATCTAGAT ATGAAGACCT CTGAGGACCA GCAAACTGCT
    TGTGGCACTA TCTACAATCC ACCTCTCAGC ATCAAGAAGC TGAGCCCAAT TATTGAAGAC
    AGCCGTGAAG CCACACATTC CTCTGGGTTC TCCGGATCTT CTGCCTCAGT TACAAGCACC
    TCCTCCATCA AAAGTCTTCA AATTCCTGAG AAACTGGAGC TCACTAGTGA TACTGCAGAC
    AGCCCTACTC AGTCACCCTG GTGTCCACAG TATCGCAGAC AACTGGTAAA ATCCCTACCA
    GAGCTGAGTG TCTCTACAGA GTTTTTTGTA GAAGATAGAC CAATGCCTAG TTTGGAAATA
    GAGAAGGAAA TTGAATTAGA TAATGAGGAA TATTGCATTA GACAAGAATA CCTGGTATGT
    GAAGACTACA AATTATTCTG GGTGACACCA AGAAACACTG CCGAGTTAAC CATAATAAAG
    GTATCTTCTC ATCCTGTCCC ATGGGACTTC TATATCAACT TCAAGTTAAA GGAACGTTTA
    AATGAGGAGT GTGATAATTT GTGTAGCTGT TATCAATACC AAGATGGCTG TATCGTTTGG
    CATCAATATA TAAACTGCTT CACCCTTCAG GATCTTCTTC AACACAGTGA ATTTATTACC
    CATGAAATGA CGGTATTGAT TATTTATAAC CTTTTGACAA TGGTGGAGAA ACTACACAAA
    GCAGAAATAG TCCATGGTGA CTTGAGTCCA AGGAGCCTGA TTCTTAGAAA CAGAATCCAC
    GATCCCTATG ATTTTAATAA GAACAATCAA GCTTTGAAGA TAGTGGACTT TTCCTACAGT
    GTTGACCTTA GGGCACAGCT AGACGTTTTT AACCTCAGTG GCTTTCGGAC TGTACAAACC
    CTGGAAGGAC AAAAGATCCT GGCTAACTGT TCTTCTCCAT ACCAGGTAGA TCTGTTTGGT
    ATAGCAGATT TAGCACATTT ATTATTGTTC AAGGAGCACC TACAGGTCTT CTGGGATGGT
    TCCCTCTGGA AACTTAGCCA AAATATTTCT GAGCTAAAAG ATGGTGAATT GTGGAATAAA
    TTCTTTTTGC GGATTCTGAA TGCCAGTGAT GAGTCCACAG TGTCTGTTCT GGGGGAACTT
    GCAGCAGAAA TGAATGGGGT GTTTGACACC ACATTCCAAA GTCACCTGAA CAAAGCTTTT
    TGGAAAGTGG GGAAGTTAAT CAGTCCTGGG GCTTTCCTCT TCCAGCAAGA TAGGCAGTCA
    AGCCTCTCAC AGATTCCTGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06476
    Clone ID Related Accession (Same CDS sequence) NM_001145173.1
    Accession Version NM_001145173.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3189bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-02
    Organism Bos taurus(cattle)
    Product mitotic checkpoint serine/threonine-protein kinase BUB1 beta
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000010.1. On Feb 13, 2009 this sequence version replaced XM_617181.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571262.21607.1, SRR5571257.48774.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145426, SAMN03145433 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGCGA TGCAGAAGGA AAGGGGTGCT CCGAGTGACA TCATGTCCTT GGAGGGAGAT 
    GAATGGGAGC TGAGTAAAGA AAATGTCCAA CCTTTAAGGC AGGGGCGGAT CATGTCCACA
    CTTCAGGGAG CTCTGGCACA GCAAGAATCT GCCTGTAACA CTACTCTTCA ACAACAAAAA
    CGAGCATTTG AATCTGAAAT TCGATTTTAT GCTGGAAATG ACCCTCTGGA TGTTTGGGAT
    AGATATATTA ACTGGACAGA GCAAAACTAT CCTCAGGGCG GGAAGGAAAG CAATATGTCA
    ACATTACTAG AAAGAGCTGT AGAAGCACTA CAAGGAGAAA AACGATATTA TAATGATCCT
    CGATTTCTTA GTCTCTGGCT TAAGCTGGGG CATTTATGCA ATGAACCTTT GGATATGTAC
    AGTTATTTAC ATAACCAAGG AATTGGCATT TCACTTGCTC AGTTTTATAT CTCATGGGCT
    GAAGAATATG AAGCTAGAGA AAACTTTAAG AAAGCAGATC TGATATTTCA AGAAGGTATT
    GAACAGAAGG CTGAACCACT GGAAAGACTG CAGTCCCAGC ACAGACAATT CCAAGCTCGA
    GTGTCTCGGC AAACTCTGTT GGCACTTGAG AAAGAAGAGG AGGAGGAAGT TGTGGGATCT
    TCTGTACCAC AGCGAAGCAC ACTGGCTGAA CTAAAAAGCA AAGGGAAAAA GACAGCAAGA
    GCTCCAATCA GCCGTGTAGG GGGTGCTCTC AAGGCTCCAA GCCAGAACAG AGGTCTCCAA
    AATCCAGTTC CTCAACAGAT GCAAAGTAAT ACTAGAATTA TTGTTTTTGA TGAAAATACT
    GATGAGGCTT CTAGAGCAGA GCCATCTAAG CCTACAGTCC AGCCATGGAT AGCACCTCCT
    GTGTCCAGGG GCAAAGAGAA TGAGCTGCAA GCCGGCCCTT GGAACACAGG CAGGCCTTTG
    GAATACAGGC CTCGAGGCAA TACAGCGTCT ATGACAGCTG TACCCTCTAT GCTTCCAAGT
    TTTACTCCAT ATGTGGAAGA GACTGCACAA CAGCCAGTTA TGACACCATG TAAAATTGAA
    CCCAGTATAA ACCACATTCT AAGCACAAGA AAGCCTGGAA AGGAAGAAGG AGATCTCCTG
    CAGAGAGTTC AAAGTCTTCA GCAAGAGTCT GAGAAGAAGG AGAAGATGAT GTATTGTAAG
    GAGAAGATTT ATGCAGGAGT GGGGGAGTTC TCCTTTGAAG AGATCCGGGC TGAGGTTTTC
    CGGAAGAAAT TAAAAGAGCG AAGAGAAGCT GAGCTACTGA CCAGTGCAGA GAAAAGAGCA
    GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA AACTACTCAG
    CAAGAAAGAG CGAATGAACA GCAAGAAGAA AAGATGCCTA CAAAGGAGAC AGCTGAACTG
    CCAATTGCTT CAGAGTCTCA GGAAATATCA GGAATGGCTC TGTCCAATTT TGTTTGCCCA
    GTAAACTCTT GTGCCAGGGA AACATCACTT GCAGAAAACG TTTGGCAAGA ACAACCTCAT
    TCTAAAAGTT CCAGTGTACC TTTCTCCATT TTTGATGAAT CTCTTCTTTC AGAAAAAAAG
    CCTGTCAGTC CTTCTGCAGC TCCCTCACGT GTTTTAGCCC AACGAAGACC TCTTGCAATT
    CTCAAAACTT CAGAAAGCAT CACCTCAAAT GAGGATGTAT CTCCAGATGT TTGTGATGAA
    TTTACAGGAA TTGAGCCCTT GAGTGAGGAT GCTATTATCA CAGGTTTCAG GAATGTAACT
    ATTTGTCCTA ACCCAGAAGA CACTTGTGAC TTCGCTAGAG CAGCTCGTTT TGCATCCACT
    CCTTTTCATG AAATAATATC CTTGAAGGAT CTCCCTTCTG ATCCTGAGAG ACTGTTGCAG
    GAAGAGGATC TAGATATGAA GACCTCTGAG GACCAGCAAA CTGCTTGTGG CACTATCTAC
    AATCCACCTC TCAGCATCAA GAAGCTGAGC CCAATTATTG AAGACAGCCG TGAAGCCACA
    CATTCCTCTG GGTTCTCCGG ATCTTCTGCC TCAGTTACAA GCACCTCCTC CATCAAAAGT
    CTTCAAATTC CTGAGAAACT GGAGCTCACT AGTGATACTG CAGACAGCCC TACTCAGTCA
    CCCTGGTGTC CACAGTATCG CAGACAACTG GTAAAATCCC TACCAGAGCT GAGTGTCTCT
    ACAGAGTTTT TTGTAGAAGA TAGACCAATG CCTAGTTTGG AAATAGAGAA GGAAATTGAA
    TTAGATAATG AGGAATATTG CATTAGACAA GAATACCTGG TATGTGAAGA CTACAAATTA
    TTCTGGGTGA CACCAAGAAA CACTGCCGAG TTAACCATAA TAAAGGTATC TTCTCATCCT
    GTCCCATGGG ACTTCTATAT CAACTTCAAG TTAAAGGAAC GTTTAAATGA GGAGTGTGAT
    AATTTGTGTA GCTGTTATCA ATACCAAGAT GGCTGTATCG TTTGGCATCA ATATATAAAC
    TGCTTCACCC TTCAGGATCT TCTTCAACAC AGTGAATTTA TTACCCATGA AATGACGGTA
    TTGATTATTT ATAACCTTTT GACAATGGTG GAGAAACTAC ACAAAGCAGA AATAGTCCAT
    GGTGACTTGA GTCCAAGGAG CCTGATTCTT AGAAACAGAA TCCACGATCC CTATGATTTT
    AATAAGAACA ATCAAGCTTT GAAGATAGTG GACTTTTCCT ACAGTGTTGA CCTTAGGGCA
    CAGCTAGACG TTTTTAACCT CAGTGGCTTT CGGACTGTAC AAACCCTGGA AGGACAAAAG
    ATCCTGGCTA ACTGTTCTTC TCCATACCAG GTAGATCTGT TTGGTATAGC AGATTTAGCA
    CATTTATTAT TGTTCAAGGA GCACCTACAG GTCTTCTGGG ATGGTTCCCT CTGGAAACTT
    AGCCAAAATA TTTCTGAGCT AAAAGATGGT GAATTGTGGA ATAAATTCTT TTTGCGGATT
    CTGAATGCCA GTGATGAGTC CACAGTGTCT GTTCTGGGGG AACTTGCAGC AGAAATGAAT
    GGGGTGTTTG ACACCACATT CCAAAGTCAC CTGAACAAAG CTTTTTGGAA AGTGGGGAAG
    TTAATCAGTC CTGGGGCTTT CCTCTTCCAG CAAGATAGGC AGTCAAGCCT CTCACAGATT
    CCTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138645.1
    CDS201..3389
    Translation

    Target ORF information:

    RefSeq Version NM_001145173.1
    Organism Bos taurus(cattle)
    Definition Bos taurus BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145173.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGGCGA TGCAGAAGGA AAGGGGTGCT CCGAGTGACA TCATGTCCTT GGAGGGAGAT 
    GAATGGGAGC TGAGTAAAGA AAATGTCCAA CCTTTAAGGC AGGGGCGGAT CATGTCCACA
    CTTCAGGGAG CTCTGGCACA GCAAGAATCT GCCTGTAACA CTACTCTTCA ACAACAAAAA
    CGAGCATTTG AATCTGAAAT TCGATTTTAT GCTGGAAATG ACCCTCTGGA TGTTTGGGAT
    AGATATATTA ACTGGACAGA GCAAAACTAT CCTCAGGGCG GGAAGGAAAG CAATATGTCA
    ACATTACTAG AAAGAGCTGT AGAAGCACTA CAAGGAGAAA AACGATATTA TAATGATCCT
    CGATTTCTTA GTCTCTGGCT TAAGCTGGGG CATTTATGCA ATGAACCTTT GGATATGTAC
    AGTTATTTAC ATAACCAAGG AATTGGCATT TCACTTGCTC AGTTTTATAT CTCATGGGCT
    GAAGAATATG AAGCTAGAGA AAACTTTAAG AAAGCAGATC TGATATTTCA AGAAGGTATT
    GAACAGAAGG CTGAACCACT GGAAAGACTG CAGTCCCAGC ACAGACAATT CCAAGCTCGA
    GTGTCTCGGC AAACTCTGTT GGCACTTGAG AAAGAAGAGG AGGAGGAAGT TGTGGGATCT
    TCTGTACCAC AGCGAAGCAC ACTGGCTGAA CTAAAAAGCA AAGGGAAAAA GACAGCAAGA
    GCTCCAATCA GCCGTGTAGG GGGTGCTCTC AAGGCTCCAA GCCAGAACAG AGGTCTCCAA
    AATCCAGTTC CTCAACAGAT GCAAAGTAAT ACTAGAATTA TTGTTTTTGA TGAAAATACT
    GATGAGGCTT CTAGAGCAGA GCCATCTAAG CCTACAGTCC AGCCATGGAT AGCACCTCCT
    GTGTCCAGGG GCAAAGAGAA TGAGCTGCAA GCCGGCCCTT GGAACACAGG CAGGCCTTTG
    GAATACAGGC CTCGAGGCAA TACAGCGTCT ATGACAGCTG TACCCTCTAT GCTTCCAAGT
    TTTACTCCAT ATGTGGAAGA GACTGCACAA CAGCCAGTTA TGACACCATG TAAAATTGAA
    CCCAGTATAA ACCACATTCT AAGCACAAGA AAGCCTGGAA AGGAAGAAGG AGATCTCCTG
    CAGAGAGTTC AAAGTCTTCA GCAAGAGTCT GAGAAGAAGG AGAAGATGAT GTATTGTAAG
    GAGAAGATTT ATGCAGGAGT GGGGGAGTTC TCCTTTGAAG AGATCCGGGC TGAGGTTTTC
    CGGAAGAAAT TAAAAGAGCG AAGAGAAGCT GAGCTACTGA CCAGTGCAGA GAAAAGAGCA
    GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA AACTACTCAG
    CAAGAAAGAG CGAATGAACA GCAAGAAGAA AAGATGCCTA CAAAGGAGAC AGCTGAACTG
    CCAATTGCTT CAGAGTCTCA GGAAATATCA GGAATGGCTC TGTCCAATTT TGTTTGCCCA
    GTAAACTCTT GTGCCAGGGA AACATCACTT GCAGAAAACG TTTGGCAAGA ACAACCTCAT
    TCTAAAAGTT CCAGTGTACC TTTCTCCATT TTTGATGAAT CTCTTCTTTC AGAAAAAAAG
    CCTGTCAGTC CTTCTGCAGC TCCCTCACGT GTTTTAGCCC AACGAAGACC TCTTGCAATT
    CTCAAAACTT CAGAAAGCAT CACCTCAAAT GAGGATGTAT CTCCAGATGT TTGTGATGAA
    TTTACAGGAA TTGAGCCCTT GAGTGAGGAT GCTATTATCA CAGGTTTCAG GAATGTAACT
    ATTTGTCCTA ACCCAGAAGA CACTTGTGAC TTCGCTAGAG CAGCTCGTTT TGCATCCACT
    CCTTTTCATG AAATAATATC CTTGAAGGAT CTCCCTTCTG ATCCTGAGAG ACTGTTGCAG
    GAAGAGGATC TAGATATGAA GACCTCTGAG GACCAGCAAA CTGCTTGTGG CACTATCTAC
    AATCCACCTC TCAGCATCAA GAAGCTGAGC CCAATTATTG AAGACAGCCG TGAAGCCACA
    CATTCCTCTG GGTTCTCCGG ATCTTCTGCC TCAGTTACAA GCACCTCCTC CATCAAAAGT
    CTTCAAATTC CTGAGAAACT GGAGCTCACT AGTGATACTG CAGACAGCCC TACTCAGTCA
    CCCTGGTGTC CACAGTATCG CAGACAACTG GTAAAATCCC TACCAGAGCT GAGTGTCTCT
    ACAGAGTTTT TTGTAGAAGA TAGACCAATG CCTAGTTTGG AAATAGAGAA GGAAATTGAA
    TTAGATAATG AGGAATATTG CATTAGACAA GAATACCTGG TATGTGAAGA CTACAAATTA
    TTCTGGGTGA CACCAAGAAA CACTGCCGAG TTAACCATAA TAAAGGTATC TTCTCATCCT
    GTCCCATGGG ACTTCTATAT CAACTTCAAG TTAAAGGAAC GTTTAAATGA GGAGTGTGAT
    AATTTGTGTA GCTGTTATCA ATACCAAGAT GGCTGTATCG TTTGGCATCA ATATATAAAC
    TGCTTCACCC TTCAGGATCT TCTTCAACAC AGTGAATTTA TTACCCATGA AATGACGGTA
    TTGATTATTT ATAACCTTTT GACAATGGTG GAGAAACTAC ACAAAGCAGA AATAGTCCAT
    GGTGACTTGA GTCCAAGGAG CCTGATTCTT AGAAACAGAA TCCACGATCC CTATGATTTT
    AATAAGAACA ATCAAGCTTT GAAGATAGTG GACTTTTCCT ACAGTGTTGA CCTTAGGGCA
    CAGCTAGACG TTTTTAACCT CAGTGGCTTT CGGACTGTAC AAACCCTGGA AGGACAAAAG
    ATCCTGGCTA ACTGTTCTTC TCCATACCAG GTAGATCTGT TTGGTATAGC AGATTTAGCA
    CATTTATTAT TGTTCAAGGA GCACCTACAG GTCTTCTGGG ATGGTTCCCT CTGGAAACTT
    AGCCAAAATA TTTCTGAGCT AAAAGATGGT GAATTGTGGA ATAAATTCTT TTTGCGGATT
    CTGAATGCCA GTGATGAGTC CACAGTGTCT GTTCTGGGGG AACTTGCAGC AGAAATGAAT
    GGGGTGTTTG ACACCACATT CCAAAGTCAC CTGAACAAAG CTTTTTGGAA AGTGGGGAAG
    TTAATCAGTC CTGGGGCTTT CCTCTTCCAG CAAGATAGGC AGTCAAGCCT CTCACAGATT
    CCTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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