TTBK1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	TTBK1A
Entrez Gene ID	100332125
Full Name	tau tubulin kinase 1a
General protein information	Preferred Names tau tubulin kinase 1a Names tau-tubulin kinase 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009306132.3	XP_009304407.1	tau-tubulin kinase 1 isoform X2
XM_009306129.2	XP_009304404.1	tau-tubulin kinase 1 isoform X1
XM_009306130.3	XP_009304405.1	tau-tubulin kinase 1 isoform X1
XM_009306130.2	XP_009304405.1	tau-tubulin kinase 1 isoform X2
XM_009306132.2	XP_009304407.1	tau-tubulin kinase 1 isoform X3
XM_009306131.3	XP_009304406.1	tau-tubulin kinase 1 isoform X2
XM_009306131.2	XP_009304406.1	tau-tubulin kinase 1 isoform X3
XM_009306133.2	XP_009304408.1	tau-tubulin kinase 1 isoform X4

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ttbk1	NP_001156336.1
Pan troglodytes (chimpanzee)	TTBK1	XP_003311367.1
Gallus gallus (chicken)	TTBK1	NP_001186368.1
Homo sapiens (human)	TTBK1	NP_115927.1
	TTBK1	XP_001088342.2
Rattus norvegicus (Norway rat)	Ttbk1	NP_001258159.1
Canis lupus familiaris (dog)	TTBK1	XP_005627821.1
Danio rerio (zebrafish)	LOC100332125	XP_002663847.3

Gene Ontology

The following TTBK1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTBK1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa49575	XM_009306133.2 Latest version!	Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa49572	XM_009306129.2 Latest version!	Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa49573	XM_009306130.3 Latest version!	Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa49574	XM_009306132.3 Latest version!	Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa49574	XM_009306131.3 Latest version!	Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa49573	XM_009306130.2	Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa49574	XM_009306132.2	Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa49574	XM_009306131.2	Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TTBK1A ( XM_009306133.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304408.1 Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa49575
Clone ID Related Accession (Same CDS sequence)	XM_009306133.2
Accession Version	XM_009306133.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tau-tubulin kinase 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306133.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAAATG GCTCATTTAA GCTGGGCGCT GCCAGAGTGC TCTCAGGGTT GCAGTGCCTA 
GCGCCAGCTC TGCAGGACAA CGCCAACATG AACGGCACTG CAGAGCAGCA GGACATTCTG 
CCCCCTCATT GCATGGTCAA AGATCGATGG AAAGTGCTGA AGAAGATCGG AGGAGGAGGG 
TTTGGGGAAA TCTACGAGGC GCTGGACCTG CTGACTCGAG AGAATGTGGC GTTAAAAGTG 
GAGTCAGCGC AACAGCCCAA ACAAGTCCTG AAGATGGAGG TAGCAGTTTT GAAGAAACTG 
CAGGGTAAAA ACCACGTATG TAAGTTCATA GGCTGTGGGC GAAACGATAA ATTCAACTAT 
GTCGTCATGC AGCTGCAGGG CAGAAACCTG GCAGATCTCA GGCGAAGTCA GCCTCGCGGC 
ACCTTTACCA TGAGCACAAC CCTCCGCTTG GGAAAACAGA TCCTCGAATC CATAGAAGCC 
ATTCACTCCG TTGGCTTTCT CCATCGAGAC ATCAAACCGT CAAATTTTGC CATGGGAAGG 
CTGCCTTCAA CATTCCGGAA ATGTTATATG CTAGATTTTG GGCTCGCAAG ACAGTATACA 
AACACCAATG GAGAAGTCAG ACCGACTTTC ATTCCTAGAA CTGTTGCAGG ATTTCGAGGG 
ACTGTTAGAT ATGCATCTGT GAATGCACAT AAAAACAAAG AAATGGGTCG CCATGATGAC 
TTATGGTCAT TGTTTTACAT GCTGGTTGAG TTTGCTGCTG GGCAGCTGCC ATGGAGGAAA 
ATCAAGGACA AGGAGCAAGT GGGTCAGATT AAGGAGCGCT ACGATCATAG GATGCTTCTT 
AAACACATGC CGTCAGAGTT TAATGTCTTC CTGGAGCATG TTTTAGCCCT TGACTACTAT 
ACCAAACCAG ACTATCAGCT CCTGATGTCA GTGTTCGAGA ACAGCATGAA GGAACGCATC 
ATCACTGAAA ACGAGCCCTA TGATTGGGAG AAAAGTGGGA CGGAGACTAT TATCTCTGCT 
ACCACACCGA CAGCAGCTCA GCAGAACACG AGACCCACAG CCGCGATGGT CGGGGTTATA 
AATGTGACCC CGATGCCAGG TGACCTTCCA AGGGAAAACA CAGATGATGT TGTACAGGAT 
GAGCACCTTA GTGACCAGGA AAACGCCCCT CCAATTCCCT CTGGACGGCC AGTTGATGGC 
AGCCAGATGC CCACAGATGC AGACGGATGG GAGGAGACAG ACTTCAACCG CAACAAACTC 
AGGATTAACC TTGGAAAGGG AGGTCAAGAA GAGGACGGTA GCCGGGGTGT GTGTCCTGTT 
TCGCCACAAA GGGGAGGTTT GGACTCTCCA GGGATGCAGG TTCACTCGAT CAGGTACCGA 
CGGGTCAACA GTCCTGAATC TGATCGCATG TCTGCTGCTG ATGGACGGGA CGGTTACGGC 
CAACGCTCTC GAATGGACAT TCTGGGTTCT CCATCTCGTC ACGTTTACTC CTCTCAGCCC 
GCTCAGATGC TCTCGGTGGA CGGTTGCAGA GGCCGTAATG AAGCCTCTGC ATCGGTGGAC 
CAGGAAGCCC ACAGCAATGC CTTCATCCGC TCAGTCCCGC TCGCAGAAGA AGAGGACTTC 
GACAGCAAAG AGTGGGTGAT CATAGACAAG GAAATGGAGC TCAGAGACTT CCACCACCTT 
CCAGGAGCCG AACCTACCAC TTCCGGCACG ACGGATGAGG AGCCGGAGGA ATTACGCCCT 
CTGGAGGAAG GAGATGATCG GAGGAGACAC AGAGGGAACG ATCCATCCGT CAGAACCAAA 
ACGTCCCATG GAGAACGGAC TACACGAGGG ATGCTACCAC TGACGGAGGA AGAGACATCC 
AGGAGGAGTG GGAAAGGCCA GTCCACGTCA ATGGAGAGCC CTGCGCAGTC ACCCTGTCAC 
TCGCTGCCCT CCGGACGGCC CCGGCGGAGA GATGCAGATA ACAACGGACC TCAGAGACAG 
CTGGACGAGG ACAGGTCACA CTCGGGACCG CGCTCCAATC AGCTTCGCCG TCTCGCTTCA 
TATGTCTTTT CGTCTTCTAC GTTGGAGACA GAGCAGTACC CGTCGTCCTT CATCCAGCGG 
AGCCGGTCCG CCGAAAGCAG CCCAGCTCAC GGTAACTTCC GCTCGGGCTC CAGACGTCAC 
GCTCCCCTGC AGCCGCCTGG ATCTCCACGC AGCAGGCACT CCATGCTCAA CGTGTCCCGT 
CTGCAGATCC AGCAGATGCT GGCCAAACTG ATGAACAAGA ACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304408.1
CDS	339..2606
Translation	MINGSFKLGAARVLSGLQCLAPALQDNANMNGTAEQQDILPPHCMVKDRWKVLKKIGGGGFGEIYEALDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKNHVCKFIGCGRNDKFNYVVMQLQGRNLADLRRSQPRGTFTMSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTFRKCYMLDFGLARQYTNTNGEVRPTFIPRTVAGFRGTVRYASVNAHKNKEMGRHDDLWSLFYMLVEFAAGQLPWRKIKDKEQVGQIKERYDHRMLLKHMPSEFNVFLEHVLALDYYTKPDYQLLMSVFENSMKERIITENEPYDWEKSGTETIISATTPTAAQQNTRPTAAMVGVINVTPMPGDLPRENTDDVVQDEHLSDQENAPPIPSGRPVDGSQMPTDADGWEETDFNRNKLRINLGKGGQEEDGSRGVCPVSPQRGGLDSPGMQVHSIRYRRVNSPESDRMSAADGRDGYGQRSRMDILGSPSRHVYSSQPAQMLSVDGCRGRNEASASVDQEAHSNAFIRSVPLAEEEDFDSKEWVIIDKEMELRDFHHLPGAEPTTSGTTDEEPEELRPLEEGDDRRRHRGNDPSVRTKTSHGERTTRGMLPLTEEETSRRSGKGQSTSMESPAQSPCHSLPSGRPRRRDADNNGPQRQLDEDRSHSGPRSNQLRRLASYVFSSSTLETEQYPSSFIQRSRSAESSPAHGNFRSGSRRHAPLQPPGSPRSRHSMLNVSRLQIQQMLAKLMNKNS

Target ORF information:

RefSeq Version	XM_009306133.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306133.2

ORF Insert Sequence:

ATGATAAATG GCTCATTTAA GCTGGGCGCT GCCAGAGTGC TCTCAGGGTT GCAGTGCCTA 
GCGCCAGCTC TGCAGGACAA CGCCAACATG AACGGCACTG CAGAGCAGCA GGACATTCTG 
CCCCCTCATT GCATGGTCAA AGATCGATGG AAAGTGCTGA AGAAGATCGG AGGAGGAGGG 
TTTGGGGAAA TCTACGAGGC GCTGGACCTG CTGACTCGAG AGAATGTGGC GTTAAAAGTG 
GAGTCAGCGC AACAGCCCAA ACAAGTCCTG AAGATGGAGG TAGCAGTTTT GAAGAAACTG 
CAGGGTAAAA ACCACGTATG TAAGTTCATA GGCTGTGGGC GAAACGATAA ATTCAACTAT 
GTCGTCATGC AGCTGCAGGG CAGAAACCTG GCAGATCTCA GGCGAAGTCA GCCTCGCGGC 
ACCTTTACCA TGAGCACAAC CCTCCGCTTG GGAAAACAGA TCCTCGAATC CATAGAAGCC 
ATTCACTCCG TTGGCTTTCT CCATCGAGAC ATCAAACCGT CAAATTTTGC CATGGGAAGG 
CTGCCTTCAA CATTCCGGAA ATGTTATATG CTAGATTTTG GGCTCGCAAG ACAGTATACA 
AACACCAATG GAGAAGTCAG ACCGACTTTC ATTCCTAGAA CTGTTGCAGG ATTTCGAGGG 
ACTGTTAGAT ATGCATCTGT GAATGCACAT AAAAACAAAG AAATGGGTCG CCATGATGAC 
TTATGGTCAT TGTTTTACAT GCTGGTTGAG TTTGCTGCTG GGCAGCTGCC ATGGAGGAAA 
ATCAAGGACA AGGAGCAAGT GGGTCAGATT AAGGAGCGCT ACGATCATAG GATGCTTCTT 
AAACACATGC CGTCAGAGTT TAATGTCTTC CTGGAGCATG TTTTAGCCCT TGACTACTAT 
ACCAAACCAG ACTATCAGCT CCTGATGTCA GTGTTCGAGA ACAGCATGAA GGAACGCATC 
ATCACTGAAA ACGAGCCCTA TGATTGGGAG AAAAGTGGGA CGGAGACTAT TATCTCTGCT 
ACCACACCGA CAGCAGCTCA GCAGAACACG AGACCCACAG CCGCGATGGT CGGGGTTATA 
AATGTGACCC CGATGCCAGG TGACCTTCCA AGGGAAAACA CAGATGATGT TGTACAGGAT 
GAGCACCTTA GTGACCAGGA AAACGCCCCT CCAATTCCCT CTGGACGGCC AGTTGATGGC 
AGCCAGATGC CCACAGATGC AGACGGATGG GAGGAGACAG ACTTCAACCG CAACAAACTC 
AGGATTAACC TTGGAAAGGG AGGTCAAGAA GAGGACGGTA GCCGGGGTGT GTGTCCTGTT 
TCGCCACAAA GGGGAGGTTT GGACTCTCCA GGGATGCAGG TTCACTCGAT CAGGTACCGA 
CGGGTCAACA GTCCTGAATC TGATCGCATG TCTGCTGCTG ATGGACGGGA CGGTTACGGC 
CAACGCTCTC GAATGGACAT TCTGGGTTCT CCATCTCGTC ACGTTTACTC CTCTCAGCCC 
GCTCAGATGC TCTCGGTGGA CGGTTGCAGA GGCCGTAATG AAGCCTCTGC ATCGGTGGAC 
CAGGAAGCCC ACAGCAATGC CTTCATCCGC TCAGTCCCGC TCGCAGAAGA AGAGGACTTC 
GACAGCAAAG AGTGGGTGAT CATAGACAAG GAAATGGAGC TCAGAGACTT CCACCACCTT 
CCAGGAGCCG AACCTACCAC TTCCGGCACG ACGGATGAGG AGCCGGAGGA ATTACGCCCT 
CTGGAGGAAG GAGATGATCG GAGGAGACAC AGAGGGAACG ATCCATCCGT CAGAACCAAA 
ACGTCCCATG GAGAACGGAC TACACGAGGG ATGCTACCAC TGACGGAGGA AGAGACATCC 
AGGAGGAGTG GGAAAGGCCA GTCCACGTCA ATGGAGAGCC CTGCGCAGTC ACCCTGTCAC 
TCGCTGCCCT CCGGACGGCC CCGGCGGAGA GATGCAGATA ACAACGGACC TCAGAGACAG 
CTGGACGAGG ACAGGTCACA CTCGGGACCG CGCTCCAATC AGCTTCGCCG TCTCGCTTCA 
TATGTCTTTT CGTCTTCTAC GTTGGAGACA GAGCAGTACC CGTCGTCCTT CATCCAGCGG 
AGCCGGTCCG CCGAAAGCAG CCCAGCTCAC GGTAACTTCC GCTCGGGCTC CAGACGTCAC 
GCTCCCCTGC AGCCGCCTGG ATCTCCACGC AGCAGGCACT CCATGCTCAA CGTGTCCCGT 
CTGCAGATCC AGCAGATGCT GGCCAAACTG ATGAACAAGA ACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTBK1A ( XM_009306129.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304404.1 Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa49572
Clone ID Related Accession (Same CDS sequence)	XM_009306129.2
Accession Version	XM_009306129.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tau-tubulin kinase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306129.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAAATG GCTCATTTAA GCTGGGCGCT GCCAGAGTGC TCTCAGGGTT GCAGTGCCTA 
GCGCCAGCTC TGCAGGACAA CGCCAACATG AACGGCACTG CAGAGCAGCA GGACATTCTG 
CCCCCTCATT GCATGGTCAA AGATCGATGG AAAGTGCTGA AGAAGATCGG AGGAGGAGGG 
TTTGGGGAAA TCTACGAGGC GCTGGACCTG CTGACTCGAG AGAATGTGGC GTTAAAAGTG 
GAGTCAGCGC AACAGCCCAA ACAAGTCCTG AAGATGGAGG TAGCAGTTTT GAAGAAACTG 
CAGGGTAAAA ACCACGTATG TAAGTTCATA GGCTGTGGGC GAAACGATAA ATTCAACTAT 
GTCGTCATGC AGCTGCAGGG CAGAAACCTG GCAGATCTCA GGCGAAGTCA GCCTCGCGGC 
ACCTTTACCA TGAGCACAAC CCTCCGCTTG GGAAAACAGA TCCTCGAATC CATAGAAGCC 
ATTCACTCCG TTGGCTTTCT CCATCGAGAC ATCAAACCGT CAAATTTTGC CATGGGAAGG 
CTGCCTTCAA CATTCCGGAA ATGTTATATG CTAGATTTTG GGCTCGCAAG ACAGTATACA 
AACACCAATG GAGAAGTCAG ACCGACTTTC ATTCCTAGAA CTGTTGCAGG ATTTCGAGGG 
ACTGTTAGAT ATGCATCTGT GAATGCACAT AAAAACAAAG AAATGGGTCG CCATGATGAC 
TTATGGTCAT TGTTTTACAT GCTGGTTGAG TTTGCTGCTG GGCAGCTGCC ATGGAGGAAA 
ATCAAGGACA AGGAGCAAGT GGGTCAGATT AAGGAGCGCT ACGATCATAG GATGCTTCTT 
AAACACATGC CGTCAGAGTT TAATGTCTTC CTGGAGCATG TTTTAGCCCT TGACTACTAT 
ACCAAACCAG ACTATCAGCT CCTGATGTCA GTGTTCGAGA ACAGCATGAA GGAACGCATC 
ATCACTGAAA ACGAGCCCTA TGATTGGGAG AAAAGTGGGA CGGAGACTAT TATCTCTGCT 
ACCACACCGA CAGCAGCTCA GCAGAACACG AGACCCACAG CCGCGATGGT CGGGGTTATA 
AATGTGACCC CGATGCCAGG TGACCTTCCA AGGGAAAACA CAGATGATGT TGTACAGGAT 
GAGCACCTTA GTGACCAGGA AAACGCCCCT CCAATTCCCT CTGGACGGCC AGTTGATGGC 
AGCCAGATGC CCACAGATGC AGACGGATGG GAGGAGACAG ACTTCAACCG CAACAAACTC 
AGGATTAACC TTGGAAAGGG AGGTCAAGAA GAGGACGGTA GCCGGGGTGT GTGTCCTGTT 
TCGCCACAAA GGGGAGGTTT GGACTCTCCA GGGATGCAGG TTCACTCGAT CAGGTACCGA 
CGGGTCAACA GTCCTGAATC TGATCGCATG TCTGCTGCTG ATGGACGGGA CGGTTACGGC 
CAACGCTCTC GAATGGACAT TCTGGGTTCT CCATCTCGTC ACGTTTACTC CTCTCAGCCC 
GCTCAGATGC TCTCGGTGGA CGGTTGCAGA GGCCGTAATG AAGCCTCTGC ATCGGTGGAC 
CAGGAAGCCC ACAGCAATGC CTTCATCCGC TCAGTCCCGC TCGCAGAAGA AGAGGACTTC 
GACAGCAAAG AGTGGGTGAT CATAGACAAG GAAATGGAGC TCAGAGACTT CCACCACCTT 
CCAGGAGCCG AACCTACCAC TTCCGGCACG ACGGATGAGG AGCCGGAGGA ATTACGCCCT 
CTGGAGGAAG GAGATGATCG GAGGAGACAC AGAGGGAACG ATCCATCCGT CAGAACCAAA 
ACGTCCCATG GAGAACGGAC TACACGAGGG ATGCTACCAC TGACGGAGGA AGAGACATCC 
AGGAGGAGTG GGAAAGGCCA GTCCACGTCA ATGGAGAGCC CTGCGCAGTC ACCCTGTCAC 
TCGCTGCCCT CCGGACGGCC CCGGCGGAGA GATGCAGATA ACAACGGACC TCAGAGACAG 
GCCCCAACCC CAAGTGAGCC TCTATTATCA CCCACTGACT ACAGGACTAA ATCCCCATCC 
TCTGAGAAGG AGATGTTCCA TATACTTCCA AAGCTGCAGG CTAAGAGAGC CGACTTCCTT 
ACAGTCATGC TTACAGGAAG CCTTCACCAG CGCCGGGCCT TCACAGCCAC ACCGCCTGAG 
ACACAAGATG AGGGGCCAAG AGATGATGAC GTCACAAAGA GCGAATCAAG CAGCGGAGCA 
AGTGAAAAGA GTACTGAACG TAGTCAAGAT GGGGCTCCAT CTACCTTGGT TGCCGATGAT 
CCACAAAAAG AGTCAAAAGA ACCAGGTTCG GTTGCTGATG TAGATCCTGA CCAAGAAGAT 
GTCTCAAACA CCCTTGTTTT GTTTTCACCA GGAGACACGA AGAAGTCACC AAATGCTATA 
ATCAGGGCTG AAGTTGATCA GGGTGTGGGG AGTTTTGATG CCCCTCAAAA TGAAAATCAA 
GGTCAATCAG AGGTCCAGAA ACAAGAGGAC ATCAAGCAAG CAGAGAGACC TATAGATGGA 
AGGCAAGCCG ATGTAACTGG GTTTGACCCA TTCATACAGT CATCTAAACC AGTGGTAACA 
ATCTCAACAA CTCCAGTGGC CTCTGCCTCA ATTGCATCAC CCCTTTTCTC CAAAGTGGAG 
CAAACTTTTG TACACATTGC AGAGACAACC CATCTGAATG TTATGTCTTC CAGTAGTGGA 
CAGGTTCTGG AAGTTTTACA GGAGGTTGAA GGCAAATTAC AGTCCAAACT GACAAAAGAC 
AAACCAGAGC CAGGAGGAGG TGCAGCTTGT GTCCAGTCTG AAGTCATCTC TCAGATTCTT 
ACAGAGGAAT CTCAGGTACC TAATGTCCTA GATGGGTCTT TGCCAAATGA ATCAACTGTA 
AAGCAAGAAA ATCCTACGGA TCCTCTATGT GTACAGTCTA TGGAACCCAT CATTCAAGAT 
TCAACAATTG ACTCCAATCA GGACGAAAAA CCAAAAGAAG AAATTCAAGA GGGGAGCACA 
GTACCTGATA TTGTTTCTAA ACCTCCAAGG CCAAGAAGCA GAAGTCGGAT TCCTATTCTA 
GTCCCGGAAG AGGAGCCAGG ATCTGAGAAA TCCCTGTCCA CCAAAGAACG AATGCGTAGA 
AGAGTCATGC AGCAAGACCT GGCCCGTCAA GTTGTGGAAA GACATCGTCA GGGTCGCTGG 
ATAAGACTGA ATTCCAGGAC ATCATCTTCG TTATCTTCTG GAGATGAGCC CAAGAGACCT 
TCAGAGACTC TTTCTACTAC AGGCTCGGAA GAAGATGTTC TTGAATCTGA TGATTCCCTA 
AACAAGCTCA AGAAGGAGGC TGAGGAAAAG CACCAAACTG GCTGGCACAG CAGGATCCCT 
CGACCAGTCA CCCCAATAAG GAAGCCTTCG ACTAAACTAG TTGTTGCACC TCCTTTGAGC 
CAACCTGAAG CTGCAAAATT AGAACAAAAA GCAAGCAATG GCCCAAAAGT ACAGTCACAA 
TCAGGATCAA GTGCTCCAGC ATTTCATGCA AGATCAAAAT CAACTCTGTC CCATTCGACC 
ATGCCAAGTG GAAGCAATTG CATGCCTCTC TCCTGTATTC CTGCAGCATC CCGCCGCAGC 
TTAAGCACCG CAAACTGCAC TTTCTCTTCA GCCCGTATAG CTAGTCCCTC GCCCCATAGG 
CTACACTTGC GAACTGCTGT TATTGAATCC CGCAAGCAAC CGTTTGCCCT CCGAACCACT 
GTGGAGCTCC CATTGAAGTC TAGGACTACT ACTTGCAGCA ATCCTGCAAA GGACACCAAA 
CCATCACCAA ACAAAGCCAC CAACACAACT GTCAGGAATC GAACCAAAGC TTCTACTCAA 
TCAAAAAACA CAGTTCCCGC TAAAAAGGAA AAAAGCACAA GCAACAATGC AGCTGCAGCC 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304404.1
CDS	333..4238
Translation	MINGSFKLGAARVLSGLQCLAPALQDNANMNGTAEQQDILPPHCMVKDRWKVLKKIGGGGFGEIYEALDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKNHVCKFIGCGRNDKFNYVVMQLQGRNLADLRRSQPRGTFTMSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTFRKCYMLDFGLARQYTNTNGEVRPTFIPRTVAGFRGTVRYASVNAHKNKEMGRHDDLWSLFYMLVEFAAGQLPWRKIKDKEQVGQIKERYDHRMLLKHMPSEFNVFLEHVLALDYYTKPDYQLLMSVFENSMKERIITENEPYDWEKSGTETIISATTPTAAQQNTRPTAAMVGVINVTPMPGDLPRENTDDVVQDEHLSDQENAPPIPSGRPVDGSQMPTDADGWEETDFNRNKLRINLGKGGQEEDGSRGVCPVSPQRGGLDSPGMQVHSIRYRRVNSPESDRMSAADGRDGYGQRSRMDILGSPSRHVYSSQPAQMLSVDGCRGRNEASASVDQEAHSNAFIRSVPLAEEEDFDSKEWVIIDKEMELRDFHHLPGAEPTTSGTTDEEPEELRPLEEGDDRRRHRGNDPSVRTKTSHGERTTRGMLPLTEEETSRRSGKGQSTSMESPAQSPCHSLPSGRPRRRDADNNGPQRQAPTPSEPLLSPTDYRTKSPSSEKEMFHILPKLQAKRADFLTVMLTGSLHQRRAFTATPPETQDEGPRDDDVTKSESSSGASEKSTERSQDGAPSTLVADDPQKESKEPGSVADVDPDQEDVSNTLVLFSPGDTKKSPNAIIRAEVDQGVGSFDAPQNENQGQSEVQKQEDIKQAERPIDGRQADVTGFDPFIQSSKPVVTISTTPVASASIASPLFSKVEQTFVHIAETTHLNVMSSSSGQVLEVLQEVEGKLQSKLTKDKPEPGGGAACVQSEVISQILTEESQVPNVLDGSLPNESTVKQENPTDPLCVQSMEPIIQDSTIDSNQDEKPKEEIQEGSTVPDIVSKPPRPRSRSRIPILVPEEEPGSEKSLSTKERMRRRVMQQDLARQVVERHRQGRWIRLNSRTSSSLSSGDEPKRPSETLSTTGSEEDVLESDDSLNKLKKEAEEKHQTGWHSRIPRPVTPIRKPSTKLVVAPPLSQPEAAKLEQKASNGPKVQSQSGSSAPAFHARSKSTLSHSTMPSGSNCMPLSCIPAASRRSLSTANCTFSSARIASPSPHRLHLRTAVIESRKQPFALRTTVELPLKSRTTTCSNPAKDTKPSPNKATNTTVRNRTKASTQSKNTVPAKKEKSTSNNAAAAR

Target ORF information:

RefSeq Version	XM_009306129.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306129.2

ORF Insert Sequence:

ATGATAAATG GCTCATTTAA GCTGGGCGCT GCCAGAGTGC TCTCAGGGTT GCAGTGCCTA 
GCGCCAGCTC TGCAGGACAA CGCCAACATG AACGGCACTG CAGAGCAGCA GGACATTCTG 
CCCCCTCATT GCATGGTCAA AGATCGATGG AAAGTGCTGA AGAAGATCGG AGGAGGAGGG 
TTTGGGGAAA TCTACGAGGC GCTGGACCTG CTGACTCGAG AGAATGTGGC GTTAAAAGTG 
GAGTCAGCGC AACAGCCCAA ACAAGTCCTG AAGATGGAGG TAGCAGTTTT GAAGAAACTG 
CAGGGTAAAA ACCACGTATG TAAGTTCATA GGCTGTGGGC GAAACGATAA ATTCAACTAT 
GTCGTCATGC AGCTGCAGGG CAGAAACCTG GCAGATCTCA GGCGAAGTCA GCCTCGCGGC 
ACCTTTACCA TGAGCACAAC CCTCCGCTTG GGAAAACAGA TCCTCGAATC CATAGAAGCC 
ATTCACTCCG TTGGCTTTCT CCATCGAGAC ATCAAACCGT CAAATTTTGC CATGGGAAGG 
CTGCCTTCAA CATTCCGGAA ATGTTATATG CTAGATTTTG GGCTCGCAAG ACAGTATACA 
AACACCAATG GAGAAGTCAG ACCGACTTTC ATTCCTAGAA CTGTTGCAGG ATTTCGAGGG 
ACTGTTAGAT ATGCATCTGT GAATGCACAT AAAAACAAAG AAATGGGTCG CCATGATGAC 
TTATGGTCAT TGTTTTACAT GCTGGTTGAG TTTGCTGCTG GGCAGCTGCC ATGGAGGAAA 
ATCAAGGACA AGGAGCAAGT GGGTCAGATT AAGGAGCGCT ACGATCATAG GATGCTTCTT 
AAACACATGC CGTCAGAGTT TAATGTCTTC CTGGAGCATG TTTTAGCCCT TGACTACTAT 
ACCAAACCAG ACTATCAGCT CCTGATGTCA GTGTTCGAGA ACAGCATGAA GGAACGCATC 
ATCACTGAAA ACGAGCCCTA TGATTGGGAG AAAAGTGGGA CGGAGACTAT TATCTCTGCT 
ACCACACCGA CAGCAGCTCA GCAGAACACG AGACCCACAG CCGCGATGGT CGGGGTTATA 
AATGTGACCC CGATGCCAGG TGACCTTCCA AGGGAAAACA CAGATGATGT TGTACAGGAT 
GAGCACCTTA GTGACCAGGA AAACGCCCCT CCAATTCCCT CTGGACGGCC AGTTGATGGC 
AGCCAGATGC CCACAGATGC AGACGGATGG GAGGAGACAG ACTTCAACCG CAACAAACTC 
AGGATTAACC TTGGAAAGGG AGGTCAAGAA GAGGACGGTA GCCGGGGTGT GTGTCCTGTT 
TCGCCACAAA GGGGAGGTTT GGACTCTCCA GGGATGCAGG TTCACTCGAT CAGGTACCGA 
CGGGTCAACA GTCCTGAATC TGATCGCATG TCTGCTGCTG ATGGACGGGA CGGTTACGGC 
CAACGCTCTC GAATGGACAT TCTGGGTTCT CCATCTCGTC ACGTTTACTC CTCTCAGCCC 
GCTCAGATGC TCTCGGTGGA CGGTTGCAGA GGCCGTAATG AAGCCTCTGC ATCGGTGGAC 
CAGGAAGCCC ACAGCAATGC CTTCATCCGC TCAGTCCCGC TCGCAGAAGA AGAGGACTTC 
GACAGCAAAG AGTGGGTGAT CATAGACAAG GAAATGGAGC TCAGAGACTT CCACCACCTT 
CCAGGAGCCG AACCTACCAC TTCCGGCACG ACGGATGAGG AGCCGGAGGA ATTACGCCCT 
CTGGAGGAAG GAGATGATCG GAGGAGACAC AGAGGGAACG ATCCATCCGT CAGAACCAAA 
ACGTCCCATG GAGAACGGAC TACACGAGGG ATGCTACCAC TGACGGAGGA AGAGACATCC 
AGGAGGAGTG GGAAAGGCCA GTCCACGTCA ATGGAGAGCC CTGCGCAGTC ACCCTGTCAC 
TCGCTGCCCT CCGGACGGCC CCGGCGGAGA GATGCAGATA ACAACGGACC TCAGAGACAG 
GCCCCAACCC CAAGTGAGCC TCTATTATCA CCCACTGACT ACAGGACTAA ATCCCCATCC 
TCTGAGAAGG AGATGTTCCA TATACTTCCA AAGCTGCAGG CTAAGAGAGC CGACTTCCTT 
ACAGTCATGC TTACAGGAAG CCTTCACCAG CGCCGGGCCT TCACAGCCAC ACCGCCTGAG 
ACACAAGATG AGGGGCCAAG AGATGATGAC GTCACAAAGA GCGAATCAAG CAGCGGAGCA 
AGTGAAAAGA GTACTGAACG TAGTCAAGAT GGGGCTCCAT CTACCTTGGT TGCCGATGAT 
CCACAAAAAG AGTCAAAAGA ACCAGGTTCG GTTGCTGATG TAGATCCTGA CCAAGAAGAT 
GTCTCAAACA CCCTTGTTTT GTTTTCACCA GGAGACACGA AGAAGTCACC AAATGCTATA 
ATCAGGGCTG AAGTTGATCA GGGTGTGGGG AGTTTTGATG CCCCTCAAAA TGAAAATCAA 
GGTCAATCAG AGGTCCAGAA ACAAGAGGAC ATCAAGCAAG CAGAGAGACC TATAGATGGA 
AGGCAAGCCG ATGTAACTGG GTTTGACCCA TTCATACAGT CATCTAAACC AGTGGTAACA 
ATCTCAACAA CTCCAGTGGC CTCTGCCTCA ATTGCATCAC CCCTTTTCTC CAAAGTGGAG 
CAAACTTTTG TACACATTGC AGAGACAACC CATCTGAATG TTATGTCTTC CAGTAGTGGA 
CAGGTTCTGG AAGTTTTACA GGAGGTTGAA GGCAAATTAC AGTCCAAACT GACAAAAGAC 
AAACCAGAGC CAGGAGGAGG TGCAGCTTGT GTCCAGTCTG AAGTCATCTC TCAGATTCTT 
ACAGAGGAAT CTCAGGTACC TAATGTCCTA GATGGGTCTT TGCCAAATGA ATCAACTGTA 
AAGCAAGAAA ATCCTACGGA TCCTCTATGT GTACAGTCTA TGGAACCCAT CATTCAAGAT 
TCAACAATTG ACTCCAATCA GGACGAAAAA CCAAAAGAAG AAATTCAAGA GGGGAGCACA 
GTACCTGATA TTGTTTCTAA ACCTCCAAGG CCAAGAAGCA GAAGTCGGAT TCCTATTCTA 
GTCCCGGAAG AGGAGCCAGG ATCTGAGAAA TCCCTGTCCA CCAAAGAACG AATGCGTAGA 
AGAGTCATGC AGCAAGACCT GGCCCGTCAA GTTGTGGAAA GACATCGTCA GGGTCGCTGG 
ATAAGACTGA ATTCCAGGAC ATCATCTTCG TTATCTTCTG GAGATGAGCC CAAGAGACCT 
TCAGAGACTC TTTCTACTAC AGGCTCGGAA GAAGATGTTC TTGAATCTGA TGATTCCCTA 
AACAAGCTCA AGAAGGAGGC TGAGGAAAAG CACCAAACTG GCTGGCACAG CAGGATCCCT 
CGACCAGTCA CCCCAATAAG GAAGCCTTCG ACTAAACTAG TTGTTGCACC TCCTTTGAGC 
CAACCTGAAG CTGCAAAATT AGAACAAAAA GCAAGCAATG GCCCAAAAGT ACAGTCACAA 
TCAGGATCAA GTGCTCCAGC ATTTCATGCA AGATCAAAAT CAACTCTGTC CCATTCGACC 
ATGCCAAGTG GAAGCAATTG CATGCCTCTC TCCTGTATTC CTGCAGCATC CCGCCGCAGC 
TTAAGCACCG CAAACTGCAC TTTCTCTTCA GCCCGTATAG CTAGTCCCTC GCCCCATAGG 
CTACACTTGC GAACTGCTGT TATTGAATCC CGCAAGCAAC CGTTTGCCCT CCGAACCACT 
GTGGAGCTCC CATTGAAGTC TAGGACTACT ACTTGCAGCA ATCCTGCAAA GGACACCAAA 
CCATCACCAA ACAAAGCCAC CAACACAACT GTCAGGAATC GAACCAAAGC TTCTACTCAA 
TCAAAAAACA CAGTTCCCGC TAAAAAGGAA AAAAGCACAA GCAACAATGC AGCTGCAGCC 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTBK1A ( XM_009306130.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304405.1 Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa49573
Clone ID Related Accession (Same CDS sequence)	XM_009306130.3 , XM_009306130.2
Accession Version	XM_009306130.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tau-tubulin kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306130.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAAATG GCTCATTTAA GCTGGGCGCT GCCAGAGTGC TCTCAGGGTT GCAGTGCCTA 
GCGCCAGCTC TGCAGGACAA CGCCAACATG AACGGCACTG CAGAGCAGCA GGACATTCTG 
CCCCCTCATT GCATGGTCAA AGATCGATGG AAAGTGCTGA AGAAGATCGG AGGAGGAGGG 
TTTGGGGAAA TCTACGAGGC GCTGGACCTG CTGACTCGAG AGAATGTGGC GTTAAAAGTG 
GAGTCAGCGC AACAGCCCAA ACAAGTCCTG AAGATGGAGG TAGCAGTTTT GAAGAAACTG 
CAGGGTAAAA ACCACGTATG TAAGTTCATA GGCTGTGGGC GAAACGATAA ATTCAACTAT 
GTCGTCATGC AGCTGCAGGG CAGAAACCTG GCAGATCTCA GGCGAAGTCA GCCTCGCGGC 
ACCTTTACCA TGAGCACAAC CCTCCGCTTG GGAAAACAGA TCCTCGAATC CATAGAAGCC 
ATTCACTCCG TTGGCTTTCT CCATCGAGAC ATCAAACCGT CAAATTTTGC CATGGGAAGG 
CTGCCTTCAA CATTCCGGAA ATGTTATATG CTAGATTTTG GGCTCGCAAG ACAGTATACA 
AACACCAATG GAGAAGTCAG ACCGCCTAGA ACTGTTGCAG GATTTCGAGG GACTGTTAGA 
TATGCATCTG TGAATGCACA TAAAAACAAA GAAATGGGTC GCCATGATGA CTTATGGTCA 
TTGTTTTACA TGCTGGTTGA GTTTGCTGCT GGGCAGCTGC CATGGAGGAA AATCAAGGAC 
AAGGAGCAAG TGGGTCAGAT TAAGGAGCGC TACGATCATA GGATGCTTCT TAAACACATG 
CCGTCAGAGT TTAATGTCTT CCTGGAGCAT GTTTTAGCCC TTGACTACTA TACCAAACCA 
GACTATCAGC TCCTGATGTC AGTGTTCGAG AACAGCATGA AGGAACGCAT CATCACTGAA 
AACGAGCCCT ATGATTGGGA GAAAAGTGGG ACGGAGACTA TTATCTCTGC TACCACACCG 
ACAGCAGCTC AGCAGAACAC GAGACCCACA GCCGCGATGG TCGGGGTTAT AAATGTGACC 
CCGATGCCAG GTGACCTTCC AAGGGAAAAC ACAGATGATG TTGTACAGGA TGAGCACCTT 
AGTGACCAGG AAAACGCCCC TCCAATTCCC TCTGGACGGC CAGTTGATGG CAGCCAGATG 
CCCACAGATG CAGACGGATG GGAGGAGACA GACTTCAACC GCAACAAACT CAGGATTAAC 
CTTGGAAAGG GAGGTCAAGA AGAGGACGGT AGCCGGGGTG TGTGTCCTGT TTCGCCACAA 
AGGGGAGGTT TGGACTCTCC AGGGATGCAG GTTCACTCGA TCAGGTACCG ACGGGTCAAC 
AGTCCTGAAT CTGATCGCAT GTCTGCTGCT GATGGACGGG ACGGTTACGG CCAACGCTCT 
CGAATGGACA TTCTGGGTTC TCCATCTCGT CACGTTTACT CCTCTCAGCC CGCTCAGATG 
CTCTCGGTGG ACGGTTGCAG AGGCCGTAAT GAAGCCTCTG CATCGGTGGA CCAGGAAGCC 
CACAGCAATG CCTTCATCCG CTCAGTCCCG CTCGCAGAAG AAGAGGACTT CGACAGCAAA 
GAGTGGGTGA TCATAGACAA GGAAATGGAG CTCAGAGACT TCCACCACCT TCCAGGAGCC 
GAACCTACCA CTTCCGGCAC GACGGATGAG GAGCCGGAGG AATTACGCCC TCTGGAGGAA 
GGAGATGATC GGAGGAGACA CAGAGGGAAC GATCCATCCG TCAGAACCAA AACGTCCCAT 
GGAGAACGGA CTACACGAGG GATGCTACCA CTGACGGAGG AAGAGACATC CAGGAGGAGT 
GGGAAAGGCC AGTCCACGTC AATGGAGAGC CCTGCGCAGT CACCCTGTCA CTCGCTGCCC 
TCCGGACGGC CCCGGCGGAG AGATGCAGAT AACAACGGAC CTCAGAGACA GGCCCCAACC 
CCAAGTGAGC CTCTATTATC ACCCACTGAC TACAGGACTA AATCCCCATC CTCTGAGAAG 
GAGATGTTCC ATATACTTCC AAAGCTGCAG GCTAAGAGAG CCGACTTCCT TACAGTCATG 
CTTACAGGAA GCCTTCACCA GCGCCGGGCC TTCACAGCCA CACCGCCTGA GACACAAGAT 
GAGGGGCCAA GAGATGATGA CGTCACAAAG AGCGAATCAA GCAGCGGAGC AAGTGAAAAG 
AGTACTGAAC GTAGTCAAGA TGGGGCTCCA TCTACCTTGG TTGCCGATGA TCCACAAAAA 
GAGTCAAAAG AACCAGGTTC GGTTGCTGAT GTAGATCCTG ACCAAGAAGA TGTCTCAAAC 
ACCCTTGTTT TGTTTTCACC AGGAGACACG AAGAAGTCAC CAAATGCTAT AATCAGGGCT 
GAAGTTGATC AGGGTGTGGG GAGTTTTGAT GCCCCTCAAA ATGAAAATCA AGGTCAATCA 
GAGGTCCAGA AACAAGAGGA CATCAAGCAA GCAGAGAGAC CTATAGATGG AAGGCAAGCC 
GATGTAACTG GGTTTGACCC ATTCATACAG TCATCTAAAC CAGTGGTAAC AATCTCAACA 
ACTCCAGTGG CCTCTGCCTC AATTGCATCA CCCCTTTTCT CCAAAGTGGA GCAAACTTTT 
GTACACATTG CAGAGACAAC CCATCTGAAT GTTATGTCTT CCAGTAGTGG ACAGGTTCTG 
GAAGTTTTAC AGGAGGTTGA AGGCAAATTA CAGTCCAAAC TGACAAAAGA CAAACCAGAG 
CCAGGAGGAG GTGCAGCTTG TGTCCAGTCT GAAGTCATCT CTCAGATTCT TACAGAGGAA 
TCTCAGGTAC CTAATGTCCT AGATGGGTCT TTGCCAAATG AATCAACTGT AAAGCAAGAA 
AATCCTACGG ATCCTCTATG TGTACAGTCT ATGGAACCCA TCATTCAAGA TTCAACAATT 
GACTCCAATC AGGACGAAAA ACCAAAAGAA GAAATTCAAG AGGGGAGCAC AGTACCTGAT 
ATTGTTTCTA AACCTCCAAG GCCAAGAAGC AGAAGTCGGA TTCCTATTCT AGTCCCGGAA 
GAGGAGCCAG GATCTGAGAA ATCCCTGTCC ACCAAAGAAC GAATGCGTAG AAGAGTCATG 
CAGCAAGACC TGGCCCGTCA AGTTGTGGAA AGACATCGTC AGGGTCGCTG GATAAGACTG 
AATTCCAGGA CATCATCTTC GTTATCTTCT GGAGATGAGC CCAAGAGACC TTCAGAGACT 
CTTTCTACTA CAGGCTCGGA AGAAGATGTT CTTGAATCTG ATGATTCCCT AAACAAGCTC 
AAGAAGGAGG CTGAGGAAAA GCACCAAACT GGCTGGCACA GCAGGATCCC TCGACCAGTC 
ACCCCAATAA GGAAGCCTTC GACTAAACTA GTTGTTGCAC CTCCTTTGAG CCAACCTGAA 
GCTGCAAAAT TAGAACAAAA AGCAAGCAAT GGCCCAAAAG TACAGTCACA ATCAGGATCA 
AGTGCTCCAG CATTTCATGC AAGATCAAAA TCAACTCTGT CCCATTCGAC CATGCCAAGT 
GGAAGCAATT GCATGCCTCT CTCCTGTATT CCTGCAGCAT CCCGCCGCAG CTTAAGCACC 
GCAAACTGCA CTTTCTCTTC AGCCCGTATA GCTAGTCCCT CGCCCCATAG GCTACACTTG 
CGAACTGCTG TTATTGAATC CCGCAAGCAA CCGTTTGCCC TCCGAACCAC TGTGGAGCTC 
CCATTGAAGT CTAGGACTAC TACTTGCAGC AATCCTGCAA AGGACACCAA ACCATCACCA 
AACAAAGCCA CCAACACAAC TGTCAGGAAT CGAACCAAAG CTTCTACTCA ATCAAAAAAC 
ACAGTTCCCG CTAAAAAGGA AAAAAGCACA AGCAACAATG CAGCTGCAGC CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304405.1
CDS	109..4005
Translation	MINGSFKLGAARVLSGLQCLAPALQDNANMNGTAEQQDILPPHCMVKDRWKVLKKIGGGGFGEIYEALDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKNHVCKFIGCGRNDKFNYVVMQLQGRNLADLRRSQPRGTFTMSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTFRKCYMLDFGLARQYTNTNGEVRPPRTVAGFRGTVRYASVNAHKNKEMGRHDDLWSLFYMLVEFAAGQLPWRKIKDKEQVGQIKERYDHRMLLKHMPSEFNVFLEHVLALDYYTKPDYQLLMSVFENSMKERIITENEPYDWEKSGTETIISATTPTAAQQNTRPTAAMVGVINVTPMPGDLPRENTDDVVQDEHLSDQENAPPIPSGRPVDGSQMPTDADGWEETDFNRNKLRINLGKGGQEEDGSRGVCPVSPQRGGLDSPGMQVHSIRYRRVNSPESDRMSAADGRDGYGQRSRMDILGSPSRHVYSSQPAQMLSVDGCRGRNEASASVDQEAHSNAFIRSVPLAEEEDFDSKEWVIIDKEMELRDFHHLPGAEPTTSGTTDEEPEELRPLEEGDDRRRHRGNDPSVRTKTSHGERTTRGMLPLTEEETSRRSGKGQSTSMESPAQSPCHSLPSGRPRRRDADNNGPQRQAPTPSEPLLSPTDYRTKSPSSEKEMFHILPKLQAKRADFLTVMLTGSLHQRRAFTATPPETQDEGPRDDDVTKSESSSGASEKSTERSQDGAPSTLVADDPQKESKEPGSVADVDPDQEDVSNTLVLFSPGDTKKSPNAIIRAEVDQGVGSFDAPQNENQGQSEVQKQEDIKQAERPIDGRQADVTGFDPFIQSSKPVVTISTTPVASASIASPLFSKVEQTFVHIAETTHLNVMSSSSGQVLEVLQEVEGKLQSKLTKDKPEPGGGAACVQSEVISQILTEESQVPNVLDGSLPNESTVKQENPTDPLCVQSMEPIIQDSTIDSNQDEKPKEEIQEGSTVPDIVSKPPRPRSRSRIPILVPEEEPGSEKSLSTKERMRRRVMQQDLARQVVERHRQGRWIRLNSRTSSSLSSGDEPKRPSETLSTTGSEEDVLESDDSLNKLKKEAEEKHQTGWHSRIPRPVTPIRKPSTKLVVAPPLSQPEAAKLEQKASNGPKVQSQSGSSAPAFHARSKSTLSHSTMPSGSNCMPLSCIPAASRRSLSTANCTFSSARIASPSPHRLHLRTAVIESRKQPFALRTTVELPLKSRTTTCSNPAKDTKPSPNKATNTTVRNRTKASTQSKNTVPAKKEKSTSNNAAAAR

Target ORF information:

RefSeq Version	XM_009306130.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306130.3

ORF Insert Sequence:

ATGATAAATG GCTCATTTAA GCTGGGCGCT GCCAGAGTGC TCTCAGGGTT GCAGTGCCTA 
GCGCCAGCTC TGCAGGACAA CGCCAACATG AACGGCACTG CAGAGCAGCA GGACATTCTG 
CCCCCTCATT GCATGGTCAA AGATCGATGG AAAGTGCTGA AGAAGATCGG AGGAGGAGGG 
TTTGGGGAAA TCTACGAGGC GCTGGACCTG CTGACTCGAG AGAATGTGGC GTTAAAAGTG 
GAGTCAGCGC AACAGCCCAA ACAAGTCCTG AAGATGGAGG TAGCAGTTTT GAAGAAACTG 
CAGGGTAAAA ACCACGTATG TAAGTTCATA GGCTGTGGGC GAAACGATAA ATTCAACTAT 
GTCGTCATGC AGCTGCAGGG CAGAAACCTG GCAGATCTCA GGCGAAGTCA GCCTCGCGGC 
ACCTTTACCA TGAGCACAAC CCTCCGCTTG GGAAAACAGA TCCTCGAATC CATAGAAGCC 
ATTCACTCCG TTGGCTTTCT CCATCGAGAC ATCAAACCGT CAAATTTTGC CATGGGAAGG 
CTGCCTTCAA CATTCCGGAA ATGTTATATG CTAGATTTTG GGCTCGCAAG ACAGTATACA 
AACACCAATG GAGAAGTCAG ACCGCCTAGA ACTGTTGCAG GATTTCGAGG GACTGTTAGA 
TATGCATCTG TGAATGCACA TAAAAACAAA GAAATGGGTC GCCATGATGA CTTATGGTCA 
TTGTTTTACA TGCTGGTTGA GTTTGCTGCT GGGCAGCTGC CATGGAGGAA AATCAAGGAC 
AAGGAGCAAG TGGGTCAGAT TAAGGAGCGC TACGATCATA GGATGCTTCT TAAACACATG 
CCGTCAGAGT TTAATGTCTT CCTGGAGCAT GTTTTAGCCC TTGACTACTA TACCAAACCA 
GACTATCAGC TCCTGATGTC AGTGTTCGAG AACAGCATGA AGGAACGCAT CATCACTGAA 
AACGAGCCCT ATGATTGGGA GAAAAGTGGG ACGGAGACTA TTATCTCTGC TACCACACCG 
ACAGCAGCTC AGCAGAACAC GAGACCCACA GCCGCGATGG TCGGGGTTAT AAATGTGACC 
CCGATGCCAG GTGACCTTCC AAGGGAAAAC ACAGATGATG TTGTACAGGA TGAGCACCTT 
AGTGACCAGG AAAACGCCCC TCCAATTCCC TCTGGACGGC CAGTTGATGG CAGCCAGATG 
CCCACAGATG CAGACGGATG GGAGGAGACA GACTTCAACC GCAACAAACT CAGGATTAAC 
CTTGGAAAGG GAGGTCAAGA AGAGGACGGT AGCCGGGGTG TGTGTCCTGT TTCGCCACAA 
AGGGGAGGTT TGGACTCTCC AGGGATGCAG GTTCACTCGA TCAGGTACCG ACGGGTCAAC 
AGTCCTGAAT CTGATCGCAT GTCTGCTGCT GATGGACGGG ACGGTTACGG CCAACGCTCT 
CGAATGGACA TTCTGGGTTC TCCATCTCGT CACGTTTACT CCTCTCAGCC CGCTCAGATG 
CTCTCGGTGG ACGGTTGCAG AGGCCGTAAT GAAGCCTCTG CATCGGTGGA CCAGGAAGCC 
CACAGCAATG CCTTCATCCG CTCAGTCCCG CTCGCAGAAG AAGAGGACTT CGACAGCAAA 
GAGTGGGTGA TCATAGACAA GGAAATGGAG CTCAGAGACT TCCACCACCT TCCAGGAGCC 
GAACCTACCA CTTCCGGCAC GACGGATGAG GAGCCGGAGG AATTACGCCC TCTGGAGGAA 
GGAGATGATC GGAGGAGACA CAGAGGGAAC GATCCATCCG TCAGAACCAA AACGTCCCAT 
GGAGAACGGA CTACACGAGG GATGCTACCA CTGACGGAGG AAGAGACATC CAGGAGGAGT 
GGGAAAGGCC AGTCCACGTC AATGGAGAGC CCTGCGCAGT CACCCTGTCA CTCGCTGCCC 
TCCGGACGGC CCCGGCGGAG AGATGCAGAT AACAACGGAC CTCAGAGACA GGCCCCAACC 
CCAAGTGAGC CTCTATTATC ACCCACTGAC TACAGGACTA AATCCCCATC CTCTGAGAAG 
GAGATGTTCC ATATACTTCC AAAGCTGCAG GCTAAGAGAG CCGACTTCCT TACAGTCATG 
CTTACAGGAA GCCTTCACCA GCGCCGGGCC TTCACAGCCA CACCGCCTGA GACACAAGAT 
GAGGGGCCAA GAGATGATGA CGTCACAAAG AGCGAATCAA GCAGCGGAGC AAGTGAAAAG 
AGTACTGAAC GTAGTCAAGA TGGGGCTCCA TCTACCTTGG TTGCCGATGA TCCACAAAAA 
GAGTCAAAAG AACCAGGTTC GGTTGCTGAT GTAGATCCTG ACCAAGAAGA TGTCTCAAAC 
ACCCTTGTTT TGTTTTCACC AGGAGACACG AAGAAGTCAC CAAATGCTAT AATCAGGGCT 
GAAGTTGATC AGGGTGTGGG GAGTTTTGAT GCCCCTCAAA ATGAAAATCA AGGTCAATCA 
GAGGTCCAGA AACAAGAGGA CATCAAGCAA GCAGAGAGAC CTATAGATGG AAGGCAAGCC 
GATGTAACTG GGTTTGACCC ATTCATACAG TCATCTAAAC CAGTGGTAAC AATCTCAACA 
ACTCCAGTGG CCTCTGCCTC AATTGCATCA CCCCTTTTCT CCAAAGTGGA GCAAACTTTT 
GTACACATTG CAGAGACAAC CCATCTGAAT GTTATGTCTT CCAGTAGTGG ACAGGTTCTG 
GAAGTTTTAC AGGAGGTTGA AGGCAAATTA CAGTCCAAAC TGACAAAAGA CAAACCAGAG 
CCAGGAGGAG GTGCAGCTTG TGTCCAGTCT GAAGTCATCT CTCAGATTCT TACAGAGGAA 
TCTCAGGTAC CTAATGTCCT AGATGGGTCT TTGCCAAATG AATCAACTGT AAAGCAAGAA 
AATCCTACGG ATCCTCTATG TGTACAGTCT ATGGAACCCA TCATTCAAGA TTCAACAATT 
GACTCCAATC AGGACGAAAA ACCAAAAGAA GAAATTCAAG AGGGGAGCAC AGTACCTGAT 
ATTGTTTCTA AACCTCCAAG GCCAAGAAGC AGAAGTCGGA TTCCTATTCT AGTCCCGGAA 
GAGGAGCCAG GATCTGAGAA ATCCCTGTCC ACCAAAGAAC GAATGCGTAG AAGAGTCATG 
CAGCAAGACC TGGCCCGTCA AGTTGTGGAA AGACATCGTC AGGGTCGCTG GATAAGACTG 
AATTCCAGGA CATCATCTTC GTTATCTTCT GGAGATGAGC CCAAGAGACC TTCAGAGACT 
CTTTCTACTA CAGGCTCGGA AGAAGATGTT CTTGAATCTG ATGATTCCCT AAACAAGCTC 
AAGAAGGAGG CTGAGGAAAA GCACCAAACT GGCTGGCACA GCAGGATCCC TCGACCAGTC 
ACCCCAATAA GGAAGCCTTC GACTAAACTA GTTGTTGCAC CTCCTTTGAG CCAACCTGAA 
GCTGCAAAAT TAGAACAAAA AGCAAGCAAT GGCCCAAAAG TACAGTCACA ATCAGGATCA 
AGTGCTCCAG CATTTCATGC AAGATCAAAA TCAACTCTGT CCCATTCGAC CATGCCAAGT 
GGAAGCAATT GCATGCCTCT CTCCTGTATT CCTGCAGCAT CCCGCCGCAG CTTAAGCACC 
GCAAACTGCA CTTTCTCTTC AGCCCGTATA GCTAGTCCCT CGCCCCATAG GCTACACTTG 
CGAACTGCTG TTATTGAATC CCGCAAGCAA CCGTTTGCCC TCCGAACCAC TGTGGAGCTC 
CCATTGAAGT CTAGGACTAC TACTTGCAGC AATCCTGCAA AGGACACCAA ACCATCACCA 
AACAAAGCCA CCAACACAAC TGTCAGGAAT CGAACCAAAG CTTCTACTCA ATCAAAAAAC 
ACAGTTCCCG CTAAAAAGGA AAAAAGCACA AGCAACAATG CAGCTGCAGC CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTBK1A ( XM_009306130.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304405.1 Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa49573
Clone ID Related Accession (Same CDS sequence)	XM_009306130.3 , XM_009306130.2
Accession Version	XM_009306130.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tau-tubulin kinase 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306130.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAAATG GCTCATTTAA GCTGGGCGCT GCCAGAGTGC TCTCAGGGTT GCAGTGCCTA 
GCGCCAGCTC TGCAGGACAA CGCCAACATG AACGGCACTG CAGAGCAGCA GGACATTCTG 
CCCCCTCATT GCATGGTCAA AGATCGATGG AAAGTGCTGA AGAAGATCGG AGGAGGAGGG 
TTTGGGGAAA TCTACGAGGC GCTGGACCTG CTGACTCGAG AGAATGTGGC GTTAAAAGTG 
GAGTCAGCGC AACAGCCCAA ACAAGTCCTG AAGATGGAGG TAGCAGTTTT GAAGAAACTG 
CAGGGTAAAA ACCACGTATG TAAGTTCATA GGCTGTGGGC GAAACGATAA ATTCAACTAT 
GTCGTCATGC AGCTGCAGGG CAGAAACCTG GCAGATCTCA GGCGAAGTCA GCCTCGCGGC 
ACCTTTACCA TGAGCACAAC CCTCCGCTTG GGAAAACAGA TCCTCGAATC CATAGAAGCC 
ATTCACTCCG TTGGCTTTCT CCATCGAGAC ATCAAACCGT CAAATTTTGC CATGGGAAGG 
CTGCCTTCAA CATTCCGGAA ATGTTATATG CTAGATTTTG GGCTCGCAAG ACAGTATACA 
AACACCAATG GAGAAGTCAG ACCGCCTAGA ACTGTTGCAG GATTTCGAGG GACTGTTAGA 
TATGCATCTG TGAATGCACA TAAAAACAAA GAAATGGGTC GCCATGATGA CTTATGGTCA 
TTGTTTTACA TGCTGGTTGA GTTTGCTGCT GGGCAGCTGC CATGGAGGAA AATCAAGGAC 
AAGGAGCAAG TGGGTCAGAT TAAGGAGCGC TACGATCATA GGATGCTTCT TAAACACATG 
CCGTCAGAGT TTAATGTCTT CCTGGAGCAT GTTTTAGCCC TTGACTACTA TACCAAACCA 
GACTATCAGC TCCTGATGTC AGTGTTCGAG AACAGCATGA AGGAACGCAT CATCACTGAA 
AACGAGCCCT ATGATTGGGA GAAAAGTGGG ACGGAGACTA TTATCTCTGC TACCACACCG 
ACAGCAGCTC AGCAGAACAC GAGACCCACA GCCGCGATGG TCGGGGTTAT AAATGTGACC 
CCGATGCCAG GTGACCTTCC AAGGGAAAAC ACAGATGATG TTGTACAGGA TGAGCACCTT 
AGTGACCAGG AAAACGCCCC TCCAATTCCC TCTGGACGGC CAGTTGATGG CAGCCAGATG 
CCCACAGATG CAGACGGATG GGAGGAGACA GACTTCAACC GCAACAAACT CAGGATTAAC 
CTTGGAAAGG GAGGTCAAGA AGAGGACGGT AGCCGGGGTG TGTGTCCTGT TTCGCCACAA 
AGGGGAGGTT TGGACTCTCC AGGGATGCAG GTTCACTCGA TCAGGTACCG ACGGGTCAAC 
AGTCCTGAAT CTGATCGCAT GTCTGCTGCT GATGGACGGG ACGGTTACGG CCAACGCTCT 
CGAATGGACA TTCTGGGTTC TCCATCTCGT CACGTTTACT CCTCTCAGCC CGCTCAGATG 
CTCTCGGTGG ACGGTTGCAG AGGCCGTAAT GAAGCCTCTG CATCGGTGGA CCAGGAAGCC 
CACAGCAATG CCTTCATCCG CTCAGTCCCG CTCGCAGAAG AAGAGGACTT CGACAGCAAA 
GAGTGGGTGA TCATAGACAA GGAAATGGAG CTCAGAGACT TCCACCACCT TCCAGGAGCC 
GAACCTACCA CTTCCGGCAC GACGGATGAG GAGCCGGAGG AATTACGCCC TCTGGAGGAA 
GGAGATGATC GGAGGAGACA CAGAGGGAAC GATCCATCCG TCAGAACCAA AACGTCCCAT 
GGAGAACGGA CTACACGAGG GATGCTACCA CTGACGGAGG AAGAGACATC CAGGAGGAGT 
GGGAAAGGCC AGTCCACGTC AATGGAGAGC CCTGCGCAGT CACCCTGTCA CTCGCTGCCC 
TCCGGACGGC CCCGGCGGAG AGATGCAGAT AACAACGGAC CTCAGAGACA GGCCCCAACC 
CCAAGTGAGC CTCTATTATC ACCCACTGAC TACAGGACTA AATCCCCATC CTCTGAGAAG 
GAGATGTTCC ATATACTTCC AAAGCTGCAG GCTAAGAGAG CCGACTTCCT TACAGTCATG 
CTTACAGGAA GCCTTCACCA GCGCCGGGCC TTCACAGCCA CACCGCCTGA GACACAAGAT 
GAGGGGCCAA GAGATGATGA CGTCACAAAG AGCGAATCAA GCAGCGGAGC AAGTGAAAAG 
AGTACTGAAC GTAGTCAAGA TGGGGCTCCA TCTACCTTGG TTGCCGATGA TCCACAAAAA 
GAGTCAAAAG AACCAGGTTC GGTTGCTGAT GTAGATCCTG ACCAAGAAGA TGTCTCAAAC 
ACCCTTGTTT TGTTTTCACC AGGAGACACG AAGAAGTCAC CAAATGCTAT AATCAGGGCT 
GAAGTTGATC AGGGTGTGGG GAGTTTTGAT GCCCCTCAAA ATGAAAATCA AGGTCAATCA 
GAGGTCCAGA AACAAGAGGA CATCAAGCAA GCAGAGAGAC CTATAGATGG AAGGCAAGCC 
GATGTAACTG GGTTTGACCC ATTCATACAG TCATCTAAAC CAGTGGTAAC AATCTCAACA 
ACTCCAGTGG CCTCTGCCTC AATTGCATCA CCCCTTTTCT CCAAAGTGGA GCAAACTTTT 
GTACACATTG CAGAGACAAC CCATCTGAAT GTTATGTCTT CCAGTAGTGG ACAGGTTCTG 
GAAGTTTTAC AGGAGGTTGA AGGCAAATTA CAGTCCAAAC TGACAAAAGA CAAACCAGAG 
CCAGGAGGAG GTGCAGCTTG TGTCCAGTCT GAAGTCATCT CTCAGATTCT TACAGAGGAA 
TCTCAGGTAC CTAATGTCCT AGATGGGTCT TTGCCAAATG AATCAACTGT AAAGCAAGAA 
AATCCTACGG ATCCTCTATG TGTACAGTCT ATGGAACCCA TCATTCAAGA TTCAACAATT 
GACTCCAATC AGGACGAAAA ACCAAAAGAA GAAATTCAAG AGGGGAGCAC AGTACCTGAT 
ATTGTTTCTA AACCTCCAAG GCCAAGAAGC AGAAGTCGGA TTCCTATTCT AGTCCCGGAA 
GAGGAGCCAG GATCTGAGAA ATCCCTGTCC ACCAAAGAAC GAATGCGTAG AAGAGTCATG 
CAGCAAGACC TGGCCCGTCA AGTTGTGGAA AGACATCGTC AGGGTCGCTG GATAAGACTG 
AATTCCAGGA CATCATCTTC GTTATCTTCT GGAGATGAGC CCAAGAGACC TTCAGAGACT 
CTTTCTACTA CAGGCTCGGA AGAAGATGTT CTTGAATCTG ATGATTCCCT AAACAAGCTC 
AAGAAGGAGG CTGAGGAAAA GCACCAAACT GGCTGGCACA GCAGGATCCC TCGACCAGTC 
ACCCCAATAA GGAAGCCTTC GACTAAACTA GTTGTTGCAC CTCCTTTGAG CCAACCTGAA 
GCTGCAAAAT TAGAACAAAA AGCAAGCAAT GGCCCAAAAG TACAGTCACA ATCAGGATCA 
AGTGCTCCAG CATTTCATGC AAGATCAAAA TCAACTCTGT CCCATTCGAC CATGCCAAGT 
GGAAGCAATT GCATGCCTCT CTCCTGTATT CCTGCAGCAT CCCGCCGCAG CTTAAGCACC 
GCAAACTGCA CTTTCTCTTC AGCCCGTATA GCTAGTCCCT CGCCCCATAG GCTACACTTG 
CGAACTGCTG TTATTGAATC CCGCAAGCAA CCGTTTGCCC TCCGAACCAC TGTGGAGCTC 
CCATTGAAGT CTAGGACTAC TACTTGCAGC AATCCTGCAA AGGACACCAA ACCATCACCA 
AACAAAGCCA CCAACACAAC TGTCAGGAAT CGAACCAAAG CTTCTACTCA ATCAAAAAAC 
ACAGTTCCCG CTAAAAAGGA AAAAAGCACA AGCAACAATG CAGCTGCAGC CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304405.1
CDS	332..4228
Translation	MINGSFKLGAARVLSGLQCLAPALQDNANMNGTAEQQDILPPHCMVKDRWKVLKKIGGGGFGEIYEALDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKNHVCKFIGCGRNDKFNYVVMQLQGRNLADLRRSQPRGTFTMSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTFRKCYMLDFGLARQYTNTNGEVRPPRTVAGFRGTVRYASVNAHKNKEMGRHDDLWSLFYMLVEFAAGQLPWRKIKDKEQVGQIKERYDHRMLLKHMPSEFNVFLEHVLALDYYTKPDYQLLMSVFENSMKERIITENEPYDWEKSGTETIISATTPTAAQQNTRPTAAMVGVINVTPMPGDLPRENTDDVVQDEHLSDQENAPPIPSGRPVDGSQMPTDADGWEETDFNRNKLRINLGKGGQEEDGSRGVCPVSPQRGGLDSPGMQVHSIRYRRVNSPESDRMSAADGRDGYGQRSRMDILGSPSRHVYSSQPAQMLSVDGCRGRNEASASVDQEAHSNAFIRSVPLAEEEDFDSKEWVIIDKEMELRDFHHLPGAEPTTSGTTDEEPEELRPLEEGDDRRRHRGNDPSVRTKTSHGERTTRGMLPLTEEETSRRSGKGQSTSMESPAQSPCHSLPSGRPRRRDADNNGPQRQAPTPSEPLLSPTDYRTKSPSSEKEMFHILPKLQAKRADFLTVMLTGSLHQRRAFTATPPETQDEGPRDDDVTKSESSSGASEKSTERSQDGAPSTLVADDPQKESKEPGSVADVDPDQEDVSNTLVLFSPGDTKKSPNAIIRAEVDQGVGSFDAPQNENQGQSEVQKQEDIKQAERPIDGRQADVTGFDPFIQSSKPVVTISTTPVASASIASPLFSKVEQTFVHIAETTHLNVMSSSSGQVLEVLQEVEGKLQSKLTKDKPEPGGGAACVQSEVISQILTEESQVPNVLDGSLPNESTVKQENPTDPLCVQSMEPIIQDSTIDSNQDEKPKEEIQEGSTVPDIVSKPPRPRSRSRIPILVPEEEPGSEKSLSTKERMRRRVMQQDLARQVVERHRQGRWIRLNSRTSSSLSSGDEPKRPSETLSTTGSEEDVLESDDSLNKLKKEAEEKHQTGWHSRIPRPVTPIRKPSTKLVVAPPLSQPEAAKLEQKASNGPKVQSQSGSSAPAFHARSKSTLSHSTMPSGSNCMPLSCIPAASRRSLSTANCTFSSARIASPSPHRLHLRTAVIESRKQPFALRTTVELPLKSRTTTCSNPAKDTKPSPNKATNTTVRNRTKASTQSKNTVPAKKEKSTSNNAAAAR

Target ORF information:

RefSeq Version	XM_009306130.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306130.2

ORF Insert Sequence:

ATGATAAATG GCTCATTTAA GCTGGGCGCT GCCAGAGTGC TCTCAGGGTT GCAGTGCCTA 
GCGCCAGCTC TGCAGGACAA CGCCAACATG AACGGCACTG CAGAGCAGCA GGACATTCTG 
CCCCCTCATT GCATGGTCAA AGATCGATGG AAAGTGCTGA AGAAGATCGG AGGAGGAGGG 
TTTGGGGAAA TCTACGAGGC GCTGGACCTG CTGACTCGAG AGAATGTGGC GTTAAAAGTG 
GAGTCAGCGC AACAGCCCAA ACAAGTCCTG AAGATGGAGG TAGCAGTTTT GAAGAAACTG 
CAGGGTAAAA ACCACGTATG TAAGTTCATA GGCTGTGGGC GAAACGATAA ATTCAACTAT 
GTCGTCATGC AGCTGCAGGG CAGAAACCTG GCAGATCTCA GGCGAAGTCA GCCTCGCGGC 
ACCTTTACCA TGAGCACAAC CCTCCGCTTG GGAAAACAGA TCCTCGAATC CATAGAAGCC 
ATTCACTCCG TTGGCTTTCT CCATCGAGAC ATCAAACCGT CAAATTTTGC CATGGGAAGG 
CTGCCTTCAA CATTCCGGAA ATGTTATATG CTAGATTTTG GGCTCGCAAG ACAGTATACA 
AACACCAATG GAGAAGTCAG ACCGCCTAGA ACTGTTGCAG GATTTCGAGG GACTGTTAGA 
TATGCATCTG TGAATGCACA TAAAAACAAA GAAATGGGTC GCCATGATGA CTTATGGTCA 
TTGTTTTACA TGCTGGTTGA GTTTGCTGCT GGGCAGCTGC CATGGAGGAA AATCAAGGAC 
AAGGAGCAAG TGGGTCAGAT TAAGGAGCGC TACGATCATA GGATGCTTCT TAAACACATG 
CCGTCAGAGT TTAATGTCTT CCTGGAGCAT GTTTTAGCCC TTGACTACTA TACCAAACCA 
GACTATCAGC TCCTGATGTC AGTGTTCGAG AACAGCATGA AGGAACGCAT CATCACTGAA 
AACGAGCCCT ATGATTGGGA GAAAAGTGGG ACGGAGACTA TTATCTCTGC TACCACACCG 
ACAGCAGCTC AGCAGAACAC GAGACCCACA GCCGCGATGG TCGGGGTTAT AAATGTGACC 
CCGATGCCAG GTGACCTTCC AAGGGAAAAC ACAGATGATG TTGTACAGGA TGAGCACCTT 
AGTGACCAGG AAAACGCCCC TCCAATTCCC TCTGGACGGC CAGTTGATGG CAGCCAGATG 
CCCACAGATG CAGACGGATG GGAGGAGACA GACTTCAACC GCAACAAACT CAGGATTAAC 
CTTGGAAAGG GAGGTCAAGA AGAGGACGGT AGCCGGGGTG TGTGTCCTGT TTCGCCACAA 
AGGGGAGGTT TGGACTCTCC AGGGATGCAG GTTCACTCGA TCAGGTACCG ACGGGTCAAC 
AGTCCTGAAT CTGATCGCAT GTCTGCTGCT GATGGACGGG ACGGTTACGG CCAACGCTCT 
CGAATGGACA TTCTGGGTTC TCCATCTCGT CACGTTTACT CCTCTCAGCC CGCTCAGATG 
CTCTCGGTGG ACGGTTGCAG AGGCCGTAAT GAAGCCTCTG CATCGGTGGA CCAGGAAGCC 
CACAGCAATG CCTTCATCCG CTCAGTCCCG CTCGCAGAAG AAGAGGACTT CGACAGCAAA 
GAGTGGGTGA TCATAGACAA GGAAATGGAG CTCAGAGACT TCCACCACCT TCCAGGAGCC 
GAACCTACCA CTTCCGGCAC GACGGATGAG GAGCCGGAGG AATTACGCCC TCTGGAGGAA 
GGAGATGATC GGAGGAGACA CAGAGGGAAC GATCCATCCG TCAGAACCAA AACGTCCCAT 
GGAGAACGGA CTACACGAGG GATGCTACCA CTGACGGAGG AAGAGACATC CAGGAGGAGT 
GGGAAAGGCC AGTCCACGTC AATGGAGAGC CCTGCGCAGT CACCCTGTCA CTCGCTGCCC 
TCCGGACGGC CCCGGCGGAG AGATGCAGAT AACAACGGAC CTCAGAGACA GGCCCCAACC 
CCAAGTGAGC CTCTATTATC ACCCACTGAC TACAGGACTA AATCCCCATC CTCTGAGAAG 
GAGATGTTCC ATATACTTCC AAAGCTGCAG GCTAAGAGAG CCGACTTCCT TACAGTCATG 
CTTACAGGAA GCCTTCACCA GCGCCGGGCC TTCACAGCCA CACCGCCTGA GACACAAGAT 
GAGGGGCCAA GAGATGATGA CGTCACAAAG AGCGAATCAA GCAGCGGAGC AAGTGAAAAG 
AGTACTGAAC GTAGTCAAGA TGGGGCTCCA TCTACCTTGG TTGCCGATGA TCCACAAAAA 
GAGTCAAAAG AACCAGGTTC GGTTGCTGAT GTAGATCCTG ACCAAGAAGA TGTCTCAAAC 
ACCCTTGTTT TGTTTTCACC AGGAGACACG AAGAAGTCAC CAAATGCTAT AATCAGGGCT 
GAAGTTGATC AGGGTGTGGG GAGTTTTGAT GCCCCTCAAA ATGAAAATCA AGGTCAATCA 
GAGGTCCAGA AACAAGAGGA CATCAAGCAA GCAGAGAGAC CTATAGATGG AAGGCAAGCC 
GATGTAACTG GGTTTGACCC ATTCATACAG TCATCTAAAC CAGTGGTAAC AATCTCAACA 
ACTCCAGTGG CCTCTGCCTC AATTGCATCA CCCCTTTTCT CCAAAGTGGA GCAAACTTTT 
GTACACATTG CAGAGACAAC CCATCTGAAT GTTATGTCTT CCAGTAGTGG ACAGGTTCTG 
GAAGTTTTAC AGGAGGTTGA AGGCAAATTA CAGTCCAAAC TGACAAAAGA CAAACCAGAG 
CCAGGAGGAG GTGCAGCTTG TGTCCAGTCT GAAGTCATCT CTCAGATTCT TACAGAGGAA 
TCTCAGGTAC CTAATGTCCT AGATGGGTCT TTGCCAAATG AATCAACTGT AAAGCAAGAA 
AATCCTACGG ATCCTCTATG TGTACAGTCT ATGGAACCCA TCATTCAAGA TTCAACAATT 
GACTCCAATC AGGACGAAAA ACCAAAAGAA GAAATTCAAG AGGGGAGCAC AGTACCTGAT 
ATTGTTTCTA AACCTCCAAG GCCAAGAAGC AGAAGTCGGA TTCCTATTCT AGTCCCGGAA 
GAGGAGCCAG GATCTGAGAA ATCCCTGTCC ACCAAAGAAC GAATGCGTAG AAGAGTCATG 
CAGCAAGACC TGGCCCGTCA AGTTGTGGAA AGACATCGTC AGGGTCGCTG GATAAGACTG 
AATTCCAGGA CATCATCTTC GTTATCTTCT GGAGATGAGC CCAAGAGACC TTCAGAGACT 
CTTTCTACTA CAGGCTCGGA AGAAGATGTT CTTGAATCTG ATGATTCCCT AAACAAGCTC 
AAGAAGGAGG CTGAGGAAAA GCACCAAACT GGCTGGCACA GCAGGATCCC TCGACCAGTC 
ACCCCAATAA GGAAGCCTTC GACTAAACTA GTTGTTGCAC CTCCTTTGAG CCAACCTGAA 
GCTGCAAAAT TAGAACAAAA AGCAAGCAAT GGCCCAAAAG TACAGTCACA ATCAGGATCA 
AGTGCTCCAG CATTTCATGC AAGATCAAAA TCAACTCTGT CCCATTCGAC CATGCCAAGT 
GGAAGCAATT GCATGCCTCT CTCCTGTATT CCTGCAGCAT CCCGCCGCAG CTTAAGCACC 
GCAAACTGCA CTTTCTCTTC AGCCCGTATA GCTAGTCCCT CGCCCCATAG GCTACACTTG 
CGAACTGCTG TTATTGAATC CCGCAAGCAA CCGTTTGCCC TCCGAACCAC TGTGGAGCTC 
CCATTGAAGT CTAGGACTAC TACTTGCAGC AATCCTGCAA AGGACACCAA ACCATCACCA 
AACAAAGCCA CCAACACAAC TGTCAGGAAT CGAACCAAAG CTTCTACTCA ATCAAAAAAC 
ACAGTTCCCG CTAAAAAGGA AAAAAGCACA AGCAACAATG CAGCTGCAGC CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTBK1A ( XM_009306132.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304407.1 Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X3, mRNA.

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CloneID	ODa49574
Clone ID Related Accession (Same CDS sequence)	XM_009306132.3 , XM_009306132.2 , XM_009306131.3 , XM_009306131.2
Accession Version	XM_009306132.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tau-tubulin kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306132.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGGCA CTGCAGAGCA GCAGGACATT CTGCCCCCTC ATTGCATGGT CAAAGATCGA 
TGGAAAGTGC TGAAGAAGAT CGGAGGAGGA GGGTTTGGGG AAATCTACGA GGCGCTGGAC 
CTGCTGACTC GAGAGAATGT GGCGTTAAAA GTGGAGTCAG CGCAACAGCC CAAACAAGTC 
CTGAAGATGG AGGTAGCAGT TTTGAAGAAA CTGCAGGGTA AAAACCACGT ATGTAAGTTC 
ATAGGCTGTG GGCGAAACGA TAAATTCAAC TATGTCGTCA TGCAGCTGCA GGGCAGAAAC 
CTGGCAGATC TCAGGCGAAG TCAGCCTCGC GGCACCTTTA CCATGAGCAC AACCCTCCGC 
TTGGGAAAAC AGATCCTCGA ATCCATAGAA GCCATTCACT CCGTTGGCTT TCTCCATCGA 
GACATCAAAC CGTCAAATTT TGCCATGGGA AGGCTGCCTT CAACATTCCG GAAATGTTAT 
ATGCTAGATT TTGGGCTCGC AAGACAGTAT ACAAACACCA ATGGAGAAGT CAGACCGACT 
TTCATTCCTA GAACTGTTGC AGGATTTCGA GGGACTGTTA GATATGCATC TGTGAATGCA 
CATAAAAACA AAGAAATGGG TCGCCATGAT GACTTATGGT CATTGTTTTA CATGCTGGTT 
GAGTTTGCTG CTGGGCAGCT GCCATGGAGG AAAATCAAGG ACAAGGAGCA AGTGGGTCAG 
ATTAAGGAGC GCTACGATCA TAGGATGCTT CTTAAACACA TGCCGTCAGA GTTTAATGTC 
TTCCTGGAGC ATGTTTTAGC CCTTGACTAC TATACCAAAC CAGACTATCA GCTCCTGATG 
TCAGTGTTCG AGAACAGCAT GAAGGAACGC ATCATCACTG AAAACGAGCC CTATGATTGG 
GAGAAAAGTG GGACGGAGAC TATTATCTCT GCTACCACAC CGACAGCAGC TCAGCAGAAC 
ACGAGACCCA CAGCCGCGAT GGTCGGGGTT ATAAATGTGA CCCCGATGCC AGGTGACCTT 
CCAAGGGAAA ACACAGATGA TGTTGTACAG GATGAGCACC TTAGTGACCA GGAAAACGCC 
CCTCCAATTC CCTCTGGACG GCCAGTTGAT GGCAGCCAGA TGCCCACAGA TGCAGACGGA 
TGGGAGGAGA CAGACTTCAA CCGCAACAAA CTCAGGATTA ACCTTGGAAA GGGAGGTCAA 
GAAGAGGACG GTAGCCGGGG TGTGTGTCCT GTTTCGCCAC AAAGGGGAGG TTTGGACTCT 
CCAGGGATGC AGGTTCACTC GATCAGGTAC CGACGGGTCA ACAGTCCTGA ATCTGATCGC 
ATGTCTGCTG CTGATGGACG GGACGGTTAC GGCCAACGCT CTCGAATGGA CATTCTGGGT 
TCTCCATCTC GTCACGTTTA CTCCTCTCAG CCCGCTCAGA TGCTCTCGGT GGACGGTTGC 
AGAGGCCGTA ATGAAGCCTC TGCATCGGTG GACCAGGAAG CCCACAGCAA TGCCTTCATC 
CGCTCAGTCC CGCTCGCAGA AGAAGAGGAC TTCGACAGCA AAGAGTGGGT GATCATAGAC 
AAGGAAATGG AGCTCAGAGA CTTCCACCAC CTTCCAGGAG CCGAACCTAC CACTTCCGGC 
ACGACGGATG AGGAGCCGGA GGAATTACGC CCTCTGGAGG AAGGAGATGA TCGGAGGAGA 
CACAGAGGGA ACGATCCATC CGTCAGAACC AAAACGTCCC ATGGAGAACG GACTACACGA 
GGGATGCTAC CACTGACGGA GGAAGAGACA TCCAGGAGGA GTGGGAAAGG CCAGTCCACG 
TCAATGGAGA GCCCTGCGCA GTCACCCTGT CACTCGCTGC CCTCCGGACG GCCCCGGCGG 
AGAGATGCAG ATAACAACGG ACCTCAGAGA CAGGCCCCAA CCCCAAGTGA GCCTCTATTA 
TCACCCACTG ACTACAGGAC TAAATCCCCA TCCTCTGAGA AGGAGATGTT CCATATACTT 
CCAAAGCTGC AGGCTAAGAG AGCCGACTTC CTTACAGTCA TGCTTACAGG AAGCCTTCAC 
CAGCGCCGGG CCTTCACAGC CACACCGCCT GAGACACAAG ATGAGGGGCC AAGAGATGAT 
GACGTCACAA AGAGCGAATC AAGCAGCGGA GCAAGTGAAA AGAGTACTGA ACGTAGTCAA 
GATGGGGCTC CATCTACCTT GGTTGCCGAT GATCCACAAA AAGAGTCAAA AGAACCAGGT 
TCGGTTGCTG ATGTAGATCC TGACCAAGAA GATGTCTCAA ACACCCTTGT TTTGTTTTCA 
CCAGGAGACA CGAAGAAGTC ACCAAATGCT ATAATCAGGG CTGAAGTTGA TCAGGGTGTG 
GGGAGTTTTG ATGCCCCTCA AAATGAAAAT CAAGGTCAAT CAGAGGTCCA GAAACAAGAG 
GACATCAAGC AAGCAGAGAG ACCTATAGAT GGAAGGCAAG CCGATGTAAC TGGGTTTGAC 
CCATTCATAC AGTCATCTAA ACCAGTGGTA ACAATCTCAA CAACTCCAGT GGCCTCTGCC 
TCAATTGCAT CACCCCTTTT CTCCAAAGTG GAGCAAACTT TTGTACACAT TGCAGAGACA 
ACCCATCTGA ATGTTATGTC TTCCAGTAGT GGACAGGTTC TGGAAGTTTT ACAGGAGGTT 
GAAGGCAAAT TACAGTCCAA ACTGACAAAA GACAAACCAG AGCCAGGAGG AGGTGCAGCT 
TGTGTCCAGT CTGAAGTCAT CTCTCAGATT CTTACAGAGG AATCTCAGGT ACCTAATGTC 
CTAGATGGGT CTTTGCCAAA TGAATCAACT GTAAAGCAAG AAAATCCTAC GGATCCTCTA 
TGTGTACAGT CTATGGAACC CATCATTCAA GATTCAACAA TTGACTCCAA TCAGGACGAA 
AAACCAAAAG AAGAAATTCA AGAGGGGAGC ACAGTACCTG ATATTGTTTC TAAACCTCCA 
AGGCCAAGAA GCAGAAGTCG GATTCCTATT CTAGTCCCGG AAGAGGAGCC AGGATCTGAG 
AAATCCCTGT CCACCAAAGA ACGAATGCGT AGAAGAGTCA TGCAGCAAGA CCTGGCCCGT 
CAAGTTGTGG AAAGACATCG TCAGGGTCGC TGGATAAGAC TGAATTCCAG GACATCATCT 
TCGTTATCTT CTGGAGATGA GCCCAAGAGA CCTTCAGAGA CTCTTTCTAC TACAGGCTCG 
GAAGAAGATG TTCTTGAATC TGATGATTCC CTAAACAAGC TCAAGAAGGA GGCTGAGGAA 
AAGCACCAAA CTGGCTGGCA CAGCAGGATC CCTCGACCAG TCACCCCAAT AAGGAAGCCT 
TCGACTAAAC TAGTTGTTGC ACCTCCTTTG AGCCAACCTG AAGCTGCAAA ATTAGAACAA 
AAAGCAAGCA ATGGCCCAAA AGTACAGTCA CAATCAGGAT CAAGTGCTCC AGCATTTCAT 
GCAAGATCAA AATCAACTCT GTCCCATTCG ACCATGCCAA GTGGAAGCAA TTGCATGCCT 
CTCTCCTGTA TTCCTGCAGC ATCCCGCCGC AGCTTAAGCA CCGCAAACTG CACTTTCTCT 
TCAGCCCGTA TAGCTAGTCC CTCGCCCCAT AGGCTACACT TGCGAACTGC TGTTATTGAA 
TCCCGCAAGC AACCGTTTGC CCTCCGAACC ACTGTGGAGC TCCCATTGAA GTCTAGGACT 
ACTACTTGCA GCAATCCTGC AAAGGACACC AAACCATCAC CAAACAAAGC CACCAACACA 
ACTGTCAGGA ATCGAACCAA AGCTTCTACT CAATCAAAAA ACACAGTTCC CGCTAAAAAG 
GAAAAAAGCA CAAGCAACAA TGCAGCTGCA GCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304407.1
CDS	692..4510
Translation	MNGTAEQQDILPPHCMVKDRWKVLKKIGGGGFGEIYEALDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKNHVCKFIGCGRNDKFNYVVMQLQGRNLADLRRSQPRGTFTMSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTFRKCYMLDFGLARQYTNTNGEVRPTFIPRTVAGFRGTVRYASVNAHKNKEMGRHDDLWSLFYMLVEFAAGQLPWRKIKDKEQVGQIKERYDHRMLLKHMPSEFNVFLEHVLALDYYTKPDYQLLMSVFENSMKERIITENEPYDWEKSGTETIISATTPTAAQQNTRPTAAMVGVINVTPMPGDLPRENTDDVVQDEHLSDQENAPPIPSGRPVDGSQMPTDADGWEETDFNRNKLRINLGKGGQEEDGSRGVCPVSPQRGGLDSPGMQVHSIRYRRVNSPESDRMSAADGRDGYGQRSRMDILGSPSRHVYSSQPAQMLSVDGCRGRNEASASVDQEAHSNAFIRSVPLAEEEDFDSKEWVIIDKEMELRDFHHLPGAEPTTSGTTDEEPEELRPLEEGDDRRRHRGNDPSVRTKTSHGERTTRGMLPLTEEETSRRSGKGQSTSMESPAQSPCHSLPSGRPRRRDADNNGPQRQAPTPSEPLLSPTDYRTKSPSSEKEMFHILPKLQAKRADFLTVMLTGSLHQRRAFTATPPETQDEGPRDDDVTKSESSSGASEKSTERSQDGAPSTLVADDPQKESKEPGSVADVDPDQEDVSNTLVLFSPGDTKKSPNAIIRAEVDQGVGSFDAPQNENQGQSEVQKQEDIKQAERPIDGRQADVTGFDPFIQSSKPVVTISTTPVASASIASPLFSKVEQTFVHIAETTHLNVMSSSSGQVLEVLQEVEGKLQSKLTKDKPEPGGGAACVQSEVISQILTEESQVPNVLDGSLPNESTVKQENPTDPLCVQSMEPIIQDSTIDSNQDEKPKEEIQEGSTVPDIVSKPPRPRSRSRIPILVPEEEPGSEKSLSTKERMRRRVMQQDLARQVVERHRQGRWIRLNSRTSSSLSSGDEPKRPSETLSTTGSEEDVLESDDSLNKLKKEAEEKHQTGWHSRIPRPVTPIRKPSTKLVVAPPLSQPEAAKLEQKASNGPKVQSQSGSSAPAFHARSKSTLSHSTMPSGSNCMPLSCIPAASRRSLSTANCTFSSARIASPSPHRLHLRTAVIESRKQPFALRTTVELPLKSRTTTCSNPAKDTKPSPNKATNTTVRNRTKASTQSKNTVPAKKEKSTSNNAAAAR

Target ORF information:

RefSeq Version	XM_009306132.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306132.3

ORF Insert Sequence:

ATGAACGGCA CTGCAGAGCA GCAGGACATT CTGCCCCCTC ATTGCATGGT CAAAGATCGA 
TGGAAAGTGC TGAAGAAGAT CGGAGGAGGA GGGTTTGGGG AAATCTACGA GGCGCTGGAC 
CTGCTGACTC GAGAGAATGT GGCGTTAAAA GTGGAGTCAG CGCAACAGCC CAAACAAGTC 
CTGAAGATGG AGGTAGCAGT TTTGAAGAAA CTGCAGGGTA AAAACCACGT ATGTAAGTTC 
ATAGGCTGTG GGCGAAACGA TAAATTCAAC TATGTCGTCA TGCAGCTGCA GGGCAGAAAC 
CTGGCAGATC TCAGGCGAAG TCAGCCTCGC GGCACCTTTA CCATGAGCAC AACCCTCCGC 
TTGGGAAAAC AGATCCTCGA ATCCATAGAA GCCATTCACT CCGTTGGCTT TCTCCATCGA 
GACATCAAAC CGTCAAATTT TGCCATGGGA AGGCTGCCTT CAACATTCCG GAAATGTTAT 
ATGCTAGATT TTGGGCTCGC AAGACAGTAT ACAAACACCA ATGGAGAAGT CAGACCGACT 
TTCATTCCTA GAACTGTTGC AGGATTTCGA GGGACTGTTA GATATGCATC TGTGAATGCA 
CATAAAAACA AAGAAATGGG TCGCCATGAT GACTTATGGT CATTGTTTTA CATGCTGGTT 
GAGTTTGCTG CTGGGCAGCT GCCATGGAGG AAAATCAAGG ACAAGGAGCA AGTGGGTCAG 
ATTAAGGAGC GCTACGATCA TAGGATGCTT CTTAAACACA TGCCGTCAGA GTTTAATGTC 
TTCCTGGAGC ATGTTTTAGC CCTTGACTAC TATACCAAAC CAGACTATCA GCTCCTGATG 
TCAGTGTTCG AGAACAGCAT GAAGGAACGC ATCATCACTG AAAACGAGCC CTATGATTGG 
GAGAAAAGTG GGACGGAGAC TATTATCTCT GCTACCACAC CGACAGCAGC TCAGCAGAAC 
ACGAGACCCA CAGCCGCGAT GGTCGGGGTT ATAAATGTGA CCCCGATGCC AGGTGACCTT 
CCAAGGGAAA ACACAGATGA TGTTGTACAG GATGAGCACC TTAGTGACCA GGAAAACGCC 
CCTCCAATTC CCTCTGGACG GCCAGTTGAT GGCAGCCAGA TGCCCACAGA TGCAGACGGA 
TGGGAGGAGA CAGACTTCAA CCGCAACAAA CTCAGGATTA ACCTTGGAAA GGGAGGTCAA 
GAAGAGGACG GTAGCCGGGG TGTGTGTCCT GTTTCGCCAC AAAGGGGAGG TTTGGACTCT 
CCAGGGATGC AGGTTCACTC GATCAGGTAC CGACGGGTCA ACAGTCCTGA ATCTGATCGC 
ATGTCTGCTG CTGATGGACG GGACGGTTAC GGCCAACGCT CTCGAATGGA CATTCTGGGT 
TCTCCATCTC GTCACGTTTA CTCCTCTCAG CCCGCTCAGA TGCTCTCGGT GGACGGTTGC 
AGAGGCCGTA ATGAAGCCTC TGCATCGGTG GACCAGGAAG CCCACAGCAA TGCCTTCATC 
CGCTCAGTCC CGCTCGCAGA AGAAGAGGAC TTCGACAGCA AAGAGTGGGT GATCATAGAC 
AAGGAAATGG AGCTCAGAGA CTTCCACCAC CTTCCAGGAG CCGAACCTAC CACTTCCGGC 
ACGACGGATG AGGAGCCGGA GGAATTACGC CCTCTGGAGG AAGGAGATGA TCGGAGGAGA 
CACAGAGGGA ACGATCCATC CGTCAGAACC AAAACGTCCC ATGGAGAACG GACTACACGA 
GGGATGCTAC CACTGACGGA GGAAGAGACA TCCAGGAGGA GTGGGAAAGG CCAGTCCACG 
TCAATGGAGA GCCCTGCGCA GTCACCCTGT CACTCGCTGC CCTCCGGACG GCCCCGGCGG 
AGAGATGCAG ATAACAACGG ACCTCAGAGA CAGGCCCCAA CCCCAAGTGA GCCTCTATTA 
TCACCCACTG ACTACAGGAC TAAATCCCCA TCCTCTGAGA AGGAGATGTT CCATATACTT 
CCAAAGCTGC AGGCTAAGAG AGCCGACTTC CTTACAGTCA TGCTTACAGG AAGCCTTCAC 
CAGCGCCGGG CCTTCACAGC CACACCGCCT GAGACACAAG ATGAGGGGCC AAGAGATGAT 
GACGTCACAA AGAGCGAATC AAGCAGCGGA GCAAGTGAAA AGAGTACTGA ACGTAGTCAA 
GATGGGGCTC CATCTACCTT GGTTGCCGAT GATCCACAAA AAGAGTCAAA AGAACCAGGT 
TCGGTTGCTG ATGTAGATCC TGACCAAGAA GATGTCTCAA ACACCCTTGT TTTGTTTTCA 
CCAGGAGACA CGAAGAAGTC ACCAAATGCT ATAATCAGGG CTGAAGTTGA TCAGGGTGTG 
GGGAGTTTTG ATGCCCCTCA AAATGAAAAT CAAGGTCAAT CAGAGGTCCA GAAACAAGAG 
GACATCAAGC AAGCAGAGAG ACCTATAGAT GGAAGGCAAG CCGATGTAAC TGGGTTTGAC 
CCATTCATAC AGTCATCTAA ACCAGTGGTA ACAATCTCAA CAACTCCAGT GGCCTCTGCC 
TCAATTGCAT CACCCCTTTT CTCCAAAGTG GAGCAAACTT TTGTACACAT TGCAGAGACA 
ACCCATCTGA ATGTTATGTC TTCCAGTAGT GGACAGGTTC TGGAAGTTTT ACAGGAGGTT 
GAAGGCAAAT TACAGTCCAA ACTGACAAAA GACAAACCAG AGCCAGGAGG AGGTGCAGCT 
TGTGTCCAGT CTGAAGTCAT CTCTCAGATT CTTACAGAGG AATCTCAGGT ACCTAATGTC 
CTAGATGGGT CTTTGCCAAA TGAATCAACT GTAAAGCAAG AAAATCCTAC GGATCCTCTA 
TGTGTACAGT CTATGGAACC CATCATTCAA GATTCAACAA TTGACTCCAA TCAGGACGAA 
AAACCAAAAG AAGAAATTCA AGAGGGGAGC ACAGTACCTG ATATTGTTTC TAAACCTCCA 
AGGCCAAGAA GCAGAAGTCG GATTCCTATT CTAGTCCCGG AAGAGGAGCC AGGATCTGAG 
AAATCCCTGT CCACCAAAGA ACGAATGCGT AGAAGAGTCA TGCAGCAAGA CCTGGCCCGT 
CAAGTTGTGG AAAGACATCG TCAGGGTCGC TGGATAAGAC TGAATTCCAG GACATCATCT 
TCGTTATCTT CTGGAGATGA GCCCAAGAGA CCTTCAGAGA CTCTTTCTAC TACAGGCTCG 
GAAGAAGATG TTCTTGAATC TGATGATTCC CTAAACAAGC TCAAGAAGGA GGCTGAGGAA 
AAGCACCAAA CTGGCTGGCA CAGCAGGATC CCTCGACCAG TCACCCCAAT AAGGAAGCCT 
TCGACTAAAC TAGTTGTTGC ACCTCCTTTG AGCCAACCTG AAGCTGCAAA ATTAGAACAA 
AAAGCAAGCA ATGGCCCAAA AGTACAGTCA CAATCAGGAT CAAGTGCTCC AGCATTTCAT 
GCAAGATCAA AATCAACTCT GTCCCATTCG ACCATGCCAA GTGGAAGCAA TTGCATGCCT 
CTCTCCTGTA TTCCTGCAGC ATCCCGCCGC AGCTTAAGCA CCGCAAACTG CACTTTCTCT 
TCAGCCCGTA TAGCTAGTCC CTCGCCCCAT AGGCTACACT TGCGAACTGC TGTTATTGAA 
TCCCGCAAGC AACCGTTTGC CCTCCGAACC ACTGTGGAGC TCCCATTGAA GTCTAGGACT 
ACTACTTGCA GCAATCCTGC AAAGGACACC AAACCATCAC CAAACAAAGC CACCAACACA 
ACTGTCAGGA ATCGAACCAA AGCTTCTACT CAATCAAAAA ACACAGTTCC CGCTAAAAAG 
GAAAAAAGCA CAAGCAACAA TGCAGCTGCA GCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTBK1A ( XM_009306132.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304407.1 Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa49574
Clone ID Related Accession (Same CDS sequence)	XM_009306132.3 , XM_009306132.2 , XM_009306131.3 , XM_009306131.2
Accession Version	XM_009306132.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tau-tubulin kinase 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306132.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGGCA CTGCAGAGCA GCAGGACATT CTGCCCCCTC ATTGCATGGT CAAAGATCGA 
TGGAAAGTGC TGAAGAAGAT CGGAGGAGGA GGGTTTGGGG AAATCTACGA GGCGCTGGAC 
CTGCTGACTC GAGAGAATGT GGCGTTAAAA GTGGAGTCAG CGCAACAGCC CAAACAAGTC 
CTGAAGATGG AGGTAGCAGT TTTGAAGAAA CTGCAGGGTA AAAACCACGT ATGTAAGTTC 
ATAGGCTGTG GGCGAAACGA TAAATTCAAC TATGTCGTCA TGCAGCTGCA GGGCAGAAAC 
CTGGCAGATC TCAGGCGAAG TCAGCCTCGC GGCACCTTTA CCATGAGCAC AACCCTCCGC 
TTGGGAAAAC AGATCCTCGA ATCCATAGAA GCCATTCACT CCGTTGGCTT TCTCCATCGA 
GACATCAAAC CGTCAAATTT TGCCATGGGA AGGCTGCCTT CAACATTCCG GAAATGTTAT 
ATGCTAGATT TTGGGCTCGC AAGACAGTAT ACAAACACCA ATGGAGAAGT CAGACCGACT 
TTCATTCCTA GAACTGTTGC AGGATTTCGA GGGACTGTTA GATATGCATC TGTGAATGCA 
CATAAAAACA AAGAAATGGG TCGCCATGAT GACTTATGGT CATTGTTTTA CATGCTGGTT 
GAGTTTGCTG CTGGGCAGCT GCCATGGAGG AAAATCAAGG ACAAGGAGCA AGTGGGTCAG 
ATTAAGGAGC GCTACGATCA TAGGATGCTT CTTAAACACA TGCCGTCAGA GTTTAATGTC 
TTCCTGGAGC ATGTTTTAGC CCTTGACTAC TATACCAAAC CAGACTATCA GCTCCTGATG 
TCAGTGTTCG AGAACAGCAT GAAGGAACGC ATCATCACTG AAAACGAGCC CTATGATTGG 
GAGAAAAGTG GGACGGAGAC TATTATCTCT GCTACCACAC CGACAGCAGC TCAGCAGAAC 
ACGAGACCCA CAGCCGCGAT GGTCGGGGTT ATAAATGTGA CCCCGATGCC AGGTGACCTT 
CCAAGGGAAA ACACAGATGA TGTTGTACAG GATGAGCACC TTAGTGACCA GGAAAACGCC 
CCTCCAATTC CCTCTGGACG GCCAGTTGAT GGCAGCCAGA TGCCCACAGA TGCAGACGGA 
TGGGAGGAGA CAGACTTCAA CCGCAACAAA CTCAGGATTA ACCTTGGAAA GGGAGGTCAA 
GAAGAGGACG GTAGCCGGGG TGTGTGTCCT GTTTCGCCAC AAAGGGGAGG TTTGGACTCT 
CCAGGGATGC AGGTTCACTC GATCAGGTAC CGACGGGTCA ACAGTCCTGA ATCTGATCGC 
ATGTCTGCTG CTGATGGACG GGACGGTTAC GGCCAACGCT CTCGAATGGA CATTCTGGGT 
TCTCCATCTC GTCACGTTTA CTCCTCTCAG CCCGCTCAGA TGCTCTCGGT GGACGGTTGC 
AGAGGCCGTA ATGAAGCCTC TGCATCGGTG GACCAGGAAG CCCACAGCAA TGCCTTCATC 
CGCTCAGTCC CGCTCGCAGA AGAAGAGGAC TTCGACAGCA AAGAGTGGGT GATCATAGAC 
AAGGAAATGG AGCTCAGAGA CTTCCACCAC CTTCCAGGAG CCGAACCTAC CACTTCCGGC 
ACGACGGATG AGGAGCCGGA GGAATTACGC CCTCTGGAGG AAGGAGATGA TCGGAGGAGA 
CACAGAGGGA ACGATCCATC CGTCAGAACC AAAACGTCCC ATGGAGAACG GACTACACGA 
GGGATGCTAC CACTGACGGA GGAAGAGACA TCCAGGAGGA GTGGGAAAGG CCAGTCCACG 
TCAATGGAGA GCCCTGCGCA GTCACCCTGT CACTCGCTGC CCTCCGGACG GCCCCGGCGG 
AGAGATGCAG ATAACAACGG ACCTCAGAGA CAGGCCCCAA CCCCAAGTGA GCCTCTATTA 
TCACCCACTG ACTACAGGAC TAAATCCCCA TCCTCTGAGA AGGAGATGTT CCATATACTT 
CCAAAGCTGC AGGCTAAGAG AGCCGACTTC CTTACAGTCA TGCTTACAGG AAGCCTTCAC 
CAGCGCCGGG CCTTCACAGC CACACCGCCT GAGACACAAG ATGAGGGGCC AAGAGATGAT 
GACGTCACAA AGAGCGAATC AAGCAGCGGA GCAAGTGAAA AGAGTACTGA ACGTAGTCAA 
GATGGGGCTC CATCTACCTT GGTTGCCGAT GATCCACAAA AAGAGTCAAA AGAACCAGGT 
TCGGTTGCTG ATGTAGATCC TGACCAAGAA GATGTCTCAA ACACCCTTGT TTTGTTTTCA 
CCAGGAGACA CGAAGAAGTC ACCAAATGCT ATAATCAGGG CTGAAGTTGA TCAGGGTGTG 
GGGAGTTTTG ATGCCCCTCA AAATGAAAAT CAAGGTCAAT CAGAGGTCCA GAAACAAGAG 
GACATCAAGC AAGCAGAGAG ACCTATAGAT GGAAGGCAAG CCGATGTAAC TGGGTTTGAC 
CCATTCATAC AGTCATCTAA ACCAGTGGTA ACAATCTCAA CAACTCCAGT GGCCTCTGCC 
TCAATTGCAT CACCCCTTTT CTCCAAAGTG GAGCAAACTT TTGTACACAT TGCAGAGACA 
ACCCATCTGA ATGTTATGTC TTCCAGTAGT GGACAGGTTC TGGAAGTTTT ACAGGAGGTT 
GAAGGCAAAT TACAGTCCAA ACTGACAAAA GACAAACCAG AGCCAGGAGG AGGTGCAGCT 
TGTGTCCAGT CTGAAGTCAT CTCTCAGATT CTTACAGAGG AATCTCAGGT ACCTAATGTC 
CTAGATGGGT CTTTGCCAAA TGAATCAACT GTAAAGCAAG AAAATCCTAC GGATCCTCTA 
TGTGTACAGT CTATGGAACC CATCATTCAA GATTCAACAA TTGACTCCAA TCAGGACGAA 
AAACCAAAAG AAGAAATTCA AGAGGGGAGC ACAGTACCTG ATATTGTTTC TAAACCTCCA 
AGGCCAAGAA GCAGAAGTCG GATTCCTATT CTAGTCCCGG AAGAGGAGCC AGGATCTGAG 
AAATCCCTGT CCACCAAAGA ACGAATGCGT AGAAGAGTCA TGCAGCAAGA CCTGGCCCGT 
CAAGTTGTGG AAAGACATCG TCAGGGTCGC TGGATAAGAC TGAATTCCAG GACATCATCT 
TCGTTATCTT CTGGAGATGA GCCCAAGAGA CCTTCAGAGA CTCTTTCTAC TACAGGCTCG 
GAAGAAGATG TTCTTGAATC TGATGATTCC CTAAACAAGC TCAAGAAGGA GGCTGAGGAA 
AAGCACCAAA CTGGCTGGCA CAGCAGGATC CCTCGACCAG TCACCCCAAT AAGGAAGCCT 
TCGACTAAAC TAGTTGTTGC ACCTCCTTTG AGCCAACCTG AAGCTGCAAA ATTAGAACAA 
AAAGCAAGCA ATGGCCCAAA AGTACAGTCA CAATCAGGAT CAAGTGCTCC AGCATTTCAT 
GCAAGATCAA AATCAACTCT GTCCCATTCG ACCATGCCAA GTGGAAGCAA TTGCATGCCT 
CTCTCCTGTA TTCCTGCAGC ATCCCGCCGC AGCTTAAGCA CCGCAAACTG CACTTTCTCT 
TCAGCCCGTA TAGCTAGTCC CTCGCCCCAT AGGCTACACT TGCGAACTGC TGTTATTGAA 
TCCCGCAAGC AACCGTTTGC CCTCCGAACC ACTGTGGAGC TCCCATTGAA GTCTAGGACT 
ACTACTTGCA GCAATCCTGC AAAGGACACC AAACCATCAC CAAACAAAGC CACCAACACA 
ACTGTCAGGA ATCGAACCAA AGCTTCTACT CAATCAAAAA ACACAGTTCC CGCTAAAAAG 
GAAAAAAGCA CAAGCAACAA TGCAGCTGCA GCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304407.1
CDS	680..4498
Translation	MNGTAEQQDILPPHCMVKDRWKVLKKIGGGGFGEIYEALDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKNHVCKFIGCGRNDKFNYVVMQLQGRNLADLRRSQPRGTFTMSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTFRKCYMLDFGLARQYTNTNGEVRPTFIPRTVAGFRGTVRYASVNAHKNKEMGRHDDLWSLFYMLVEFAAGQLPWRKIKDKEQVGQIKERYDHRMLLKHMPSEFNVFLEHVLALDYYTKPDYQLLMSVFENSMKERIITENEPYDWEKSGTETIISATTPTAAQQNTRPTAAMVGVINVTPMPGDLPRENTDDVVQDEHLSDQENAPPIPSGRPVDGSQMPTDADGWEETDFNRNKLRINLGKGGQEEDGSRGVCPVSPQRGGLDSPGMQVHSIRYRRVNSPESDRMSAADGRDGYGQRSRMDILGSPSRHVYSSQPAQMLSVDGCRGRNEASASVDQEAHSNAFIRSVPLAEEEDFDSKEWVIIDKEMELRDFHHLPGAEPTTSGTTDEEPEELRPLEEGDDRRRHRGNDPSVRTKTSHGERTTRGMLPLTEEETSRRSGKGQSTSMESPAQSPCHSLPSGRPRRRDADNNGPQRQAPTPSEPLLSPTDYRTKSPSSEKEMFHILPKLQAKRADFLTVMLTGSLHQRRAFTATPPETQDEGPRDDDVTKSESSSGASEKSTERSQDGAPSTLVADDPQKESKEPGSVADVDPDQEDVSNTLVLFSPGDTKKSPNAIIRAEVDQGVGSFDAPQNENQGQSEVQKQEDIKQAERPIDGRQADVTGFDPFIQSSKPVVTISTTPVASASIASPLFSKVEQTFVHIAETTHLNVMSSSSGQVLEVLQEVEGKLQSKLTKDKPEPGGGAACVQSEVISQILTEESQVPNVLDGSLPNESTVKQENPTDPLCVQSMEPIIQDSTIDSNQDEKPKEEIQEGSTVPDIVSKPPRPRSRSRIPILVPEEEPGSEKSLSTKERMRRRVMQQDLARQVVERHRQGRWIRLNSRTSSSLSSGDEPKRPSETLSTTGSEEDVLESDDSLNKLKKEAEEKHQTGWHSRIPRPVTPIRKPSTKLVVAPPLSQPEAAKLEQKASNGPKVQSQSGSSAPAFHARSKSTLSHSTMPSGSNCMPLSCIPAASRRSLSTANCTFSSARIASPSPHRLHLRTAVIESRKQPFALRTTVELPLKSRTTTCSNPAKDTKPSPNKATNTTVRNRTKASTQSKNTVPAKKEKSTSNNAAAAR

Target ORF information:

RefSeq Version	XM_009306132.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306132.2

ORF Insert Sequence:

ATGAACGGCA CTGCAGAGCA GCAGGACATT CTGCCCCCTC ATTGCATGGT CAAAGATCGA 
TGGAAAGTGC TGAAGAAGAT CGGAGGAGGA GGGTTTGGGG AAATCTACGA GGCGCTGGAC 
CTGCTGACTC GAGAGAATGT GGCGTTAAAA GTGGAGTCAG CGCAACAGCC CAAACAAGTC 
CTGAAGATGG AGGTAGCAGT TTTGAAGAAA CTGCAGGGTA AAAACCACGT ATGTAAGTTC 
ATAGGCTGTG GGCGAAACGA TAAATTCAAC TATGTCGTCA TGCAGCTGCA GGGCAGAAAC 
CTGGCAGATC TCAGGCGAAG TCAGCCTCGC GGCACCTTTA CCATGAGCAC AACCCTCCGC 
TTGGGAAAAC AGATCCTCGA ATCCATAGAA GCCATTCACT CCGTTGGCTT TCTCCATCGA 
GACATCAAAC CGTCAAATTT TGCCATGGGA AGGCTGCCTT CAACATTCCG GAAATGTTAT 
ATGCTAGATT TTGGGCTCGC AAGACAGTAT ACAAACACCA ATGGAGAAGT CAGACCGACT 
TTCATTCCTA GAACTGTTGC AGGATTTCGA GGGACTGTTA GATATGCATC TGTGAATGCA 
CATAAAAACA AAGAAATGGG TCGCCATGAT GACTTATGGT CATTGTTTTA CATGCTGGTT 
GAGTTTGCTG CTGGGCAGCT GCCATGGAGG AAAATCAAGG ACAAGGAGCA AGTGGGTCAG 
ATTAAGGAGC GCTACGATCA TAGGATGCTT CTTAAACACA TGCCGTCAGA GTTTAATGTC 
TTCCTGGAGC ATGTTTTAGC CCTTGACTAC TATACCAAAC CAGACTATCA GCTCCTGATG 
TCAGTGTTCG AGAACAGCAT GAAGGAACGC ATCATCACTG AAAACGAGCC CTATGATTGG 
GAGAAAAGTG GGACGGAGAC TATTATCTCT GCTACCACAC CGACAGCAGC TCAGCAGAAC 
ACGAGACCCA CAGCCGCGAT GGTCGGGGTT ATAAATGTGA CCCCGATGCC AGGTGACCTT 
CCAAGGGAAA ACACAGATGA TGTTGTACAG GATGAGCACC TTAGTGACCA GGAAAACGCC 
CCTCCAATTC CCTCTGGACG GCCAGTTGAT GGCAGCCAGA TGCCCACAGA TGCAGACGGA 
TGGGAGGAGA CAGACTTCAA CCGCAACAAA CTCAGGATTA ACCTTGGAAA GGGAGGTCAA 
GAAGAGGACG GTAGCCGGGG TGTGTGTCCT GTTTCGCCAC AAAGGGGAGG TTTGGACTCT 
CCAGGGATGC AGGTTCACTC GATCAGGTAC CGACGGGTCA ACAGTCCTGA ATCTGATCGC 
ATGTCTGCTG CTGATGGACG GGACGGTTAC GGCCAACGCT CTCGAATGGA CATTCTGGGT 
TCTCCATCTC GTCACGTTTA CTCCTCTCAG CCCGCTCAGA TGCTCTCGGT GGACGGTTGC 
AGAGGCCGTA ATGAAGCCTC TGCATCGGTG GACCAGGAAG CCCACAGCAA TGCCTTCATC 
CGCTCAGTCC CGCTCGCAGA AGAAGAGGAC TTCGACAGCA AAGAGTGGGT GATCATAGAC 
AAGGAAATGG AGCTCAGAGA CTTCCACCAC CTTCCAGGAG CCGAACCTAC CACTTCCGGC 
ACGACGGATG AGGAGCCGGA GGAATTACGC CCTCTGGAGG AAGGAGATGA TCGGAGGAGA 
CACAGAGGGA ACGATCCATC CGTCAGAACC AAAACGTCCC ATGGAGAACG GACTACACGA 
GGGATGCTAC CACTGACGGA GGAAGAGACA TCCAGGAGGA GTGGGAAAGG CCAGTCCACG 
TCAATGGAGA GCCCTGCGCA GTCACCCTGT CACTCGCTGC CCTCCGGACG GCCCCGGCGG 
AGAGATGCAG ATAACAACGG ACCTCAGAGA CAGGCCCCAA CCCCAAGTGA GCCTCTATTA 
TCACCCACTG ACTACAGGAC TAAATCCCCA TCCTCTGAGA AGGAGATGTT CCATATACTT 
CCAAAGCTGC AGGCTAAGAG AGCCGACTTC CTTACAGTCA TGCTTACAGG AAGCCTTCAC 
CAGCGCCGGG CCTTCACAGC CACACCGCCT GAGACACAAG ATGAGGGGCC AAGAGATGAT 
GACGTCACAA AGAGCGAATC AAGCAGCGGA GCAAGTGAAA AGAGTACTGA ACGTAGTCAA 
GATGGGGCTC CATCTACCTT GGTTGCCGAT GATCCACAAA AAGAGTCAAA AGAACCAGGT 
TCGGTTGCTG ATGTAGATCC TGACCAAGAA GATGTCTCAA ACACCCTTGT TTTGTTTTCA 
CCAGGAGACA CGAAGAAGTC ACCAAATGCT ATAATCAGGG CTGAAGTTGA TCAGGGTGTG 
GGGAGTTTTG ATGCCCCTCA AAATGAAAAT CAAGGTCAAT CAGAGGTCCA GAAACAAGAG 
GACATCAAGC AAGCAGAGAG ACCTATAGAT GGAAGGCAAG CCGATGTAAC TGGGTTTGAC 
CCATTCATAC AGTCATCTAA ACCAGTGGTA ACAATCTCAA CAACTCCAGT GGCCTCTGCC 
TCAATTGCAT CACCCCTTTT CTCCAAAGTG GAGCAAACTT TTGTACACAT TGCAGAGACA 
ACCCATCTGA ATGTTATGTC TTCCAGTAGT GGACAGGTTC TGGAAGTTTT ACAGGAGGTT 
GAAGGCAAAT TACAGTCCAA ACTGACAAAA GACAAACCAG AGCCAGGAGG AGGTGCAGCT 
TGTGTCCAGT CTGAAGTCAT CTCTCAGATT CTTACAGAGG AATCTCAGGT ACCTAATGTC 
CTAGATGGGT CTTTGCCAAA TGAATCAACT GTAAAGCAAG AAAATCCTAC GGATCCTCTA 
TGTGTACAGT CTATGGAACC CATCATTCAA GATTCAACAA TTGACTCCAA TCAGGACGAA 
AAACCAAAAG AAGAAATTCA AGAGGGGAGC ACAGTACCTG ATATTGTTTC TAAACCTCCA 
AGGCCAAGAA GCAGAAGTCG GATTCCTATT CTAGTCCCGG AAGAGGAGCC AGGATCTGAG 
AAATCCCTGT CCACCAAAGA ACGAATGCGT AGAAGAGTCA TGCAGCAAGA CCTGGCCCGT 
CAAGTTGTGG AAAGACATCG TCAGGGTCGC TGGATAAGAC TGAATTCCAG GACATCATCT 
TCGTTATCTT CTGGAGATGA GCCCAAGAGA CCTTCAGAGA CTCTTTCTAC TACAGGCTCG 
GAAGAAGATG TTCTTGAATC TGATGATTCC CTAAACAAGC TCAAGAAGGA GGCTGAGGAA 
AAGCACCAAA CTGGCTGGCA CAGCAGGATC CCTCGACCAG TCACCCCAAT AAGGAAGCCT 
TCGACTAAAC TAGTTGTTGC ACCTCCTTTG AGCCAACCTG AAGCTGCAAA ATTAGAACAA 
AAAGCAAGCA ATGGCCCAAA AGTACAGTCA CAATCAGGAT CAAGTGCTCC AGCATTTCAT 
GCAAGATCAA AATCAACTCT GTCCCATTCG ACCATGCCAA GTGGAAGCAA TTGCATGCCT 
CTCTCCTGTA TTCCTGCAGC ATCCCGCCGC AGCTTAAGCA CCGCAAACTG CACTTTCTCT 
TCAGCCCGTA TAGCTAGTCC CTCGCCCCAT AGGCTACACT TGCGAACTGC TGTTATTGAA 
TCCCGCAAGC AACCGTTTGC CCTCCGAACC ACTGTGGAGC TCCCATTGAA GTCTAGGACT 
ACTACTTGCA GCAATCCTGC AAAGGACACC AAACCATCAC CAAACAAAGC CACCAACACA 
ACTGTCAGGA ATCGAACCAA AGCTTCTACT CAATCAAAAA ACACAGTTCC CGCTAAAAAG 
GAAAAAAGCA CAAGCAACAA TGCAGCTGCA GCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTBK1A ( XM_009306131.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304406.1 Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa49574
Clone ID Related Accession (Same CDS sequence)	XM_009306132.3 , XM_009306132.2 , XM_009306131.3 , XM_009306131.2
Accession Version	XM_009306131.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tau-tubulin kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306131.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGGCA CTGCAGAGCA GCAGGACATT CTGCCCCCTC ATTGCATGGT CAAAGATCGA 
TGGAAAGTGC TGAAGAAGAT CGGAGGAGGA GGGTTTGGGG AAATCTACGA GGCGCTGGAC 
CTGCTGACTC GAGAGAATGT GGCGTTAAAA GTGGAGTCAG CGCAACAGCC CAAACAAGTC 
CTGAAGATGG AGGTAGCAGT TTTGAAGAAA CTGCAGGGTA AAAACCACGT ATGTAAGTTC 
ATAGGCTGTG GGCGAAACGA TAAATTCAAC TATGTCGTCA TGCAGCTGCA GGGCAGAAAC 
CTGGCAGATC TCAGGCGAAG TCAGCCTCGC GGCACCTTTA CCATGAGCAC AACCCTCCGC 
TTGGGAAAAC AGATCCTCGA ATCCATAGAA GCCATTCACT CCGTTGGCTT TCTCCATCGA 
GACATCAAAC CGTCAAATTT TGCCATGGGA AGGCTGCCTT CAACATTCCG GAAATGTTAT 
ATGCTAGATT TTGGGCTCGC AAGACAGTAT ACAAACACCA ATGGAGAAGT CAGACCGACT 
TTCATTCCTA GAACTGTTGC AGGATTTCGA GGGACTGTTA GATATGCATC TGTGAATGCA 
CATAAAAACA AAGAAATGGG TCGCCATGAT GACTTATGGT CATTGTTTTA CATGCTGGTT 
GAGTTTGCTG CTGGGCAGCT GCCATGGAGG AAAATCAAGG ACAAGGAGCA AGTGGGTCAG 
ATTAAGGAGC GCTACGATCA TAGGATGCTT CTTAAACACA TGCCGTCAGA GTTTAATGTC 
TTCCTGGAGC ATGTTTTAGC CCTTGACTAC TATACCAAAC CAGACTATCA GCTCCTGATG 
TCAGTGTTCG AGAACAGCAT GAAGGAACGC ATCATCACTG AAAACGAGCC CTATGATTGG 
GAGAAAAGTG GGACGGAGAC TATTATCTCT GCTACCACAC CGACAGCAGC TCAGCAGAAC 
ACGAGACCCA CAGCCGCGAT GGTCGGGGTT ATAAATGTGA CCCCGATGCC AGGTGACCTT 
CCAAGGGAAA ACACAGATGA TGTTGTACAG GATGAGCACC TTAGTGACCA GGAAAACGCC 
CCTCCAATTC CCTCTGGACG GCCAGTTGAT GGCAGCCAGA TGCCCACAGA TGCAGACGGA 
TGGGAGGAGA CAGACTTCAA CCGCAACAAA CTCAGGATTA ACCTTGGAAA GGGAGGTCAA 
GAAGAGGACG GTAGCCGGGG TGTGTGTCCT GTTTCGCCAC AAAGGGGAGG TTTGGACTCT 
CCAGGGATGC AGGTTCACTC GATCAGGTAC CGACGGGTCA ACAGTCCTGA ATCTGATCGC 
ATGTCTGCTG CTGATGGACG GGACGGTTAC GGCCAACGCT CTCGAATGGA CATTCTGGGT 
TCTCCATCTC GTCACGTTTA CTCCTCTCAG CCCGCTCAGA TGCTCTCGGT GGACGGTTGC 
AGAGGCCGTA ATGAAGCCTC TGCATCGGTG GACCAGGAAG CCCACAGCAA TGCCTTCATC 
CGCTCAGTCC CGCTCGCAGA AGAAGAGGAC TTCGACAGCA AAGAGTGGGT GATCATAGAC 
AAGGAAATGG AGCTCAGAGA CTTCCACCAC CTTCCAGGAG CCGAACCTAC CACTTCCGGC 
ACGACGGATG AGGAGCCGGA GGAATTACGC CCTCTGGAGG AAGGAGATGA TCGGAGGAGA 
CACAGAGGGA ACGATCCATC CGTCAGAACC AAAACGTCCC ATGGAGAACG GACTACACGA 
GGGATGCTAC CACTGACGGA GGAAGAGACA TCCAGGAGGA GTGGGAAAGG CCAGTCCACG 
TCAATGGAGA GCCCTGCGCA GTCACCCTGT CACTCGCTGC CCTCCGGACG GCCCCGGCGG 
AGAGATGCAG ATAACAACGG ACCTCAGAGA CAGGCCCCAA CCCCAAGTGA GCCTCTATTA 
TCACCCACTG ACTACAGGAC TAAATCCCCA TCCTCTGAGA AGGAGATGTT CCATATACTT 
CCAAAGCTGC AGGCTAAGAG AGCCGACTTC CTTACAGTCA TGCTTACAGG AAGCCTTCAC 
CAGCGCCGGG CCTTCACAGC CACACCGCCT GAGACACAAG ATGAGGGGCC AAGAGATGAT 
GACGTCACAA AGAGCGAATC AAGCAGCGGA GCAAGTGAAA AGAGTACTGA ACGTAGTCAA 
GATGGGGCTC CATCTACCTT GGTTGCCGAT GATCCACAAA AAGAGTCAAA AGAACCAGGT 
TCGGTTGCTG ATGTAGATCC TGACCAAGAA GATGTCTCAA ACACCCTTGT TTTGTTTTCA 
CCAGGAGACA CGAAGAAGTC ACCAAATGCT ATAATCAGGG CTGAAGTTGA TCAGGGTGTG 
GGGAGTTTTG ATGCCCCTCA AAATGAAAAT CAAGGTCAAT CAGAGGTCCA GAAACAAGAG 
GACATCAAGC AAGCAGAGAG ACCTATAGAT GGAAGGCAAG CCGATGTAAC TGGGTTTGAC 
CCATTCATAC AGTCATCTAA ACCAGTGGTA ACAATCTCAA CAACTCCAGT GGCCTCTGCC 
TCAATTGCAT CACCCCTTTT CTCCAAAGTG GAGCAAACTT TTGTACACAT TGCAGAGACA 
ACCCATCTGA ATGTTATGTC TTCCAGTAGT GGACAGGTTC TGGAAGTTTT ACAGGAGGTT 
GAAGGCAAAT TACAGTCCAA ACTGACAAAA GACAAACCAG AGCCAGGAGG AGGTGCAGCT 
TGTGTCCAGT CTGAAGTCAT CTCTCAGATT CTTACAGAGG AATCTCAGGT ACCTAATGTC 
CTAGATGGGT CTTTGCCAAA TGAATCAACT GTAAAGCAAG AAAATCCTAC GGATCCTCTA 
TGTGTACAGT CTATGGAACC CATCATTCAA GATTCAACAA TTGACTCCAA TCAGGACGAA 
AAACCAAAAG AAGAAATTCA AGAGGGGAGC ACAGTACCTG ATATTGTTTC TAAACCTCCA 
AGGCCAAGAA GCAGAAGTCG GATTCCTATT CTAGTCCCGG AAGAGGAGCC AGGATCTGAG 
AAATCCCTGT CCACCAAAGA ACGAATGCGT AGAAGAGTCA TGCAGCAAGA CCTGGCCCGT 
CAAGTTGTGG AAAGACATCG TCAGGGTCGC TGGATAAGAC TGAATTCCAG GACATCATCT 
TCGTTATCTT CTGGAGATGA GCCCAAGAGA CCTTCAGAGA CTCTTTCTAC TACAGGCTCG 
GAAGAAGATG TTCTTGAATC TGATGATTCC CTAAACAAGC TCAAGAAGGA GGCTGAGGAA 
AAGCACCAAA CTGGCTGGCA CAGCAGGATC CCTCGACCAG TCACCCCAAT AAGGAAGCCT 
TCGACTAAAC TAGTTGTTGC ACCTCCTTTG AGCCAACCTG AAGCTGCAAA ATTAGAACAA 
AAAGCAAGCA ATGGCCCAAA AGTACAGTCA CAATCAGGAT CAAGTGCTCC AGCATTTCAT 
GCAAGATCAA AATCAACTCT GTCCCATTCG ACCATGCCAA GTGGAAGCAA TTGCATGCCT 
CTCTCCTGTA TTCCTGCAGC ATCCCGCCGC AGCTTAAGCA CCGCAAACTG CACTTTCTCT 
TCAGCCCGTA TAGCTAGTCC CTCGCCCCAT AGGCTACACT TGCGAACTGC TGTTATTGAA 
TCCCGCAAGC AACCGTTTGC CCTCCGAACC ACTGTGGAGC TCCCATTGAA GTCTAGGACT 
ACTACTTGCA GCAATCCTGC AAAGGACACC AAACCATCAC CAAACAAAGC CACCAACACA 
ACTGTCAGGA ATCGAACCAA AGCTTCTACT CAATCAAAAA ACACAGTTCC CGCTAAAAAG 
GAAAAAAGCA CAAGCAACAA TGCAGCTGCA GCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304406.1
CDS	296..4114
Translation	MNGTAEQQDILPPHCMVKDRWKVLKKIGGGGFGEIYEALDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKNHVCKFIGCGRNDKFNYVVMQLQGRNLADLRRSQPRGTFTMSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTFRKCYMLDFGLARQYTNTNGEVRPTFIPRTVAGFRGTVRYASVNAHKNKEMGRHDDLWSLFYMLVEFAAGQLPWRKIKDKEQVGQIKERYDHRMLLKHMPSEFNVFLEHVLALDYYTKPDYQLLMSVFENSMKERIITENEPYDWEKSGTETIISATTPTAAQQNTRPTAAMVGVINVTPMPGDLPRENTDDVVQDEHLSDQENAPPIPSGRPVDGSQMPTDADGWEETDFNRNKLRINLGKGGQEEDGSRGVCPVSPQRGGLDSPGMQVHSIRYRRVNSPESDRMSAADGRDGYGQRSRMDILGSPSRHVYSSQPAQMLSVDGCRGRNEASASVDQEAHSNAFIRSVPLAEEEDFDSKEWVIIDKEMELRDFHHLPGAEPTTSGTTDEEPEELRPLEEGDDRRRHRGNDPSVRTKTSHGERTTRGMLPLTEEETSRRSGKGQSTSMESPAQSPCHSLPSGRPRRRDADNNGPQRQAPTPSEPLLSPTDYRTKSPSSEKEMFHILPKLQAKRADFLTVMLTGSLHQRRAFTATPPETQDEGPRDDDVTKSESSSGASEKSTERSQDGAPSTLVADDPQKESKEPGSVADVDPDQEDVSNTLVLFSPGDTKKSPNAIIRAEVDQGVGSFDAPQNENQGQSEVQKQEDIKQAERPIDGRQADVTGFDPFIQSSKPVVTISTTPVASASIASPLFSKVEQTFVHIAETTHLNVMSSSSGQVLEVLQEVEGKLQSKLTKDKPEPGGGAACVQSEVISQILTEESQVPNVLDGSLPNESTVKQENPTDPLCVQSMEPIIQDSTIDSNQDEKPKEEIQEGSTVPDIVSKPPRPRSRSRIPILVPEEEPGSEKSLSTKERMRRRVMQQDLARQVVERHRQGRWIRLNSRTSSSLSSGDEPKRPSETLSTTGSEEDVLESDDSLNKLKKEAEEKHQTGWHSRIPRPVTPIRKPSTKLVVAPPLSQPEAAKLEQKASNGPKVQSQSGSSAPAFHARSKSTLSHSTMPSGSNCMPLSCIPAASRRSLSTANCTFSSARIASPSPHRLHLRTAVIESRKQPFALRTTVELPLKSRTTTCSNPAKDTKPSPNKATNTTVRNRTKASTQSKNTVPAKKEKSTSNNAAAAR

Target ORF information:

RefSeq Version	XM_009306131.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306131.3

ORF Insert Sequence:

ATGAACGGCA CTGCAGAGCA GCAGGACATT CTGCCCCCTC ATTGCATGGT CAAAGATCGA 
TGGAAAGTGC TGAAGAAGAT CGGAGGAGGA GGGTTTGGGG AAATCTACGA GGCGCTGGAC 
CTGCTGACTC GAGAGAATGT GGCGTTAAAA GTGGAGTCAG CGCAACAGCC CAAACAAGTC 
CTGAAGATGG AGGTAGCAGT TTTGAAGAAA CTGCAGGGTA AAAACCACGT ATGTAAGTTC 
ATAGGCTGTG GGCGAAACGA TAAATTCAAC TATGTCGTCA TGCAGCTGCA GGGCAGAAAC 
CTGGCAGATC TCAGGCGAAG TCAGCCTCGC GGCACCTTTA CCATGAGCAC AACCCTCCGC 
TTGGGAAAAC AGATCCTCGA ATCCATAGAA GCCATTCACT CCGTTGGCTT TCTCCATCGA 
GACATCAAAC CGTCAAATTT TGCCATGGGA AGGCTGCCTT CAACATTCCG GAAATGTTAT 
ATGCTAGATT TTGGGCTCGC AAGACAGTAT ACAAACACCA ATGGAGAAGT CAGACCGACT 
TTCATTCCTA GAACTGTTGC AGGATTTCGA GGGACTGTTA GATATGCATC TGTGAATGCA 
CATAAAAACA AAGAAATGGG TCGCCATGAT GACTTATGGT CATTGTTTTA CATGCTGGTT 
GAGTTTGCTG CTGGGCAGCT GCCATGGAGG AAAATCAAGG ACAAGGAGCA AGTGGGTCAG 
ATTAAGGAGC GCTACGATCA TAGGATGCTT CTTAAACACA TGCCGTCAGA GTTTAATGTC 
TTCCTGGAGC ATGTTTTAGC CCTTGACTAC TATACCAAAC CAGACTATCA GCTCCTGATG 
TCAGTGTTCG AGAACAGCAT GAAGGAACGC ATCATCACTG AAAACGAGCC CTATGATTGG 
GAGAAAAGTG GGACGGAGAC TATTATCTCT GCTACCACAC CGACAGCAGC TCAGCAGAAC 
ACGAGACCCA CAGCCGCGAT GGTCGGGGTT ATAAATGTGA CCCCGATGCC AGGTGACCTT 
CCAAGGGAAA ACACAGATGA TGTTGTACAG GATGAGCACC TTAGTGACCA GGAAAACGCC 
CCTCCAATTC CCTCTGGACG GCCAGTTGAT GGCAGCCAGA TGCCCACAGA TGCAGACGGA 
TGGGAGGAGA CAGACTTCAA CCGCAACAAA CTCAGGATTA ACCTTGGAAA GGGAGGTCAA 
GAAGAGGACG GTAGCCGGGG TGTGTGTCCT GTTTCGCCAC AAAGGGGAGG TTTGGACTCT 
CCAGGGATGC AGGTTCACTC GATCAGGTAC CGACGGGTCA ACAGTCCTGA ATCTGATCGC 
ATGTCTGCTG CTGATGGACG GGACGGTTAC GGCCAACGCT CTCGAATGGA CATTCTGGGT 
TCTCCATCTC GTCACGTTTA CTCCTCTCAG CCCGCTCAGA TGCTCTCGGT GGACGGTTGC 
AGAGGCCGTA ATGAAGCCTC TGCATCGGTG GACCAGGAAG CCCACAGCAA TGCCTTCATC 
CGCTCAGTCC CGCTCGCAGA AGAAGAGGAC TTCGACAGCA AAGAGTGGGT GATCATAGAC 
AAGGAAATGG AGCTCAGAGA CTTCCACCAC CTTCCAGGAG CCGAACCTAC CACTTCCGGC 
ACGACGGATG AGGAGCCGGA GGAATTACGC CCTCTGGAGG AAGGAGATGA TCGGAGGAGA 
CACAGAGGGA ACGATCCATC CGTCAGAACC AAAACGTCCC ATGGAGAACG GACTACACGA 
GGGATGCTAC CACTGACGGA GGAAGAGACA TCCAGGAGGA GTGGGAAAGG CCAGTCCACG 
TCAATGGAGA GCCCTGCGCA GTCACCCTGT CACTCGCTGC CCTCCGGACG GCCCCGGCGG 
AGAGATGCAG ATAACAACGG ACCTCAGAGA CAGGCCCCAA CCCCAAGTGA GCCTCTATTA 
TCACCCACTG ACTACAGGAC TAAATCCCCA TCCTCTGAGA AGGAGATGTT CCATATACTT 
CCAAAGCTGC AGGCTAAGAG AGCCGACTTC CTTACAGTCA TGCTTACAGG AAGCCTTCAC 
CAGCGCCGGG CCTTCACAGC CACACCGCCT GAGACACAAG ATGAGGGGCC AAGAGATGAT 
GACGTCACAA AGAGCGAATC AAGCAGCGGA GCAAGTGAAA AGAGTACTGA ACGTAGTCAA 
GATGGGGCTC CATCTACCTT GGTTGCCGAT GATCCACAAA AAGAGTCAAA AGAACCAGGT 
TCGGTTGCTG ATGTAGATCC TGACCAAGAA GATGTCTCAA ACACCCTTGT TTTGTTTTCA 
CCAGGAGACA CGAAGAAGTC ACCAAATGCT ATAATCAGGG CTGAAGTTGA TCAGGGTGTG 
GGGAGTTTTG ATGCCCCTCA AAATGAAAAT CAAGGTCAAT CAGAGGTCCA GAAACAAGAG 
GACATCAAGC AAGCAGAGAG ACCTATAGAT GGAAGGCAAG CCGATGTAAC TGGGTTTGAC 
CCATTCATAC AGTCATCTAA ACCAGTGGTA ACAATCTCAA CAACTCCAGT GGCCTCTGCC 
TCAATTGCAT CACCCCTTTT CTCCAAAGTG GAGCAAACTT TTGTACACAT TGCAGAGACA 
ACCCATCTGA ATGTTATGTC TTCCAGTAGT GGACAGGTTC TGGAAGTTTT ACAGGAGGTT 
GAAGGCAAAT TACAGTCCAA ACTGACAAAA GACAAACCAG AGCCAGGAGG AGGTGCAGCT 
TGTGTCCAGT CTGAAGTCAT CTCTCAGATT CTTACAGAGG AATCTCAGGT ACCTAATGTC 
CTAGATGGGT CTTTGCCAAA TGAATCAACT GTAAAGCAAG AAAATCCTAC GGATCCTCTA 
TGTGTACAGT CTATGGAACC CATCATTCAA GATTCAACAA TTGACTCCAA TCAGGACGAA 
AAACCAAAAG AAGAAATTCA AGAGGGGAGC ACAGTACCTG ATATTGTTTC TAAACCTCCA 
AGGCCAAGAA GCAGAAGTCG GATTCCTATT CTAGTCCCGG AAGAGGAGCC AGGATCTGAG 
AAATCCCTGT CCACCAAAGA ACGAATGCGT AGAAGAGTCA TGCAGCAAGA CCTGGCCCGT 
CAAGTTGTGG AAAGACATCG TCAGGGTCGC TGGATAAGAC TGAATTCCAG GACATCATCT 
TCGTTATCTT CTGGAGATGA GCCCAAGAGA CCTTCAGAGA CTCTTTCTAC TACAGGCTCG 
GAAGAAGATG TTCTTGAATC TGATGATTCC CTAAACAAGC TCAAGAAGGA GGCTGAGGAA 
AAGCACCAAA CTGGCTGGCA CAGCAGGATC CCTCGACCAG TCACCCCAAT AAGGAAGCCT 
TCGACTAAAC TAGTTGTTGC ACCTCCTTTG AGCCAACCTG AAGCTGCAAA ATTAGAACAA 
AAAGCAAGCA ATGGCCCAAA AGTACAGTCA CAATCAGGAT CAAGTGCTCC AGCATTTCAT 
GCAAGATCAA AATCAACTCT GTCCCATTCG ACCATGCCAA GTGGAAGCAA TTGCATGCCT 
CTCTCCTGTA TTCCTGCAGC ATCCCGCCGC AGCTTAAGCA CCGCAAACTG CACTTTCTCT 
TCAGCCCGTA TAGCTAGTCC CTCGCCCCAT AGGCTACACT TGCGAACTGC TGTTATTGAA 
TCCCGCAAGC AACCGTTTGC CCTCCGAACC ACTGTGGAGC TCCCATTGAA GTCTAGGACT 
ACTACTTGCA GCAATCCTGC AAAGGACACC AAACCATCAC CAAACAAAGC CACCAACACA 
ACTGTCAGGA ATCGAACCAA AGCTTCTACT CAATCAAAAA ACACAGTTCC CGCTAAAAAG 
GAAAAAAGCA CAAGCAACAA TGCAGCTGCA GCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTBK1A ( XM_009306131.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304406.1 Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X3, mRNA.

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CloneID	ODa49574
Clone ID Related Accession (Same CDS sequence)	XM_009306132.3 , XM_009306132.2 , XM_009306131.3 , XM_009306131.2
Accession Version	XM_009306131.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tau-tubulin kinase 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306131.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGGCA CTGCAGAGCA GCAGGACATT CTGCCCCCTC ATTGCATGGT CAAAGATCGA 
TGGAAAGTGC TGAAGAAGAT CGGAGGAGGA GGGTTTGGGG AAATCTACGA GGCGCTGGAC 
CTGCTGACTC GAGAGAATGT GGCGTTAAAA GTGGAGTCAG CGCAACAGCC CAAACAAGTC 
CTGAAGATGG AGGTAGCAGT TTTGAAGAAA CTGCAGGGTA AAAACCACGT ATGTAAGTTC 
ATAGGCTGTG GGCGAAACGA TAAATTCAAC TATGTCGTCA TGCAGCTGCA GGGCAGAAAC 
CTGGCAGATC TCAGGCGAAG TCAGCCTCGC GGCACCTTTA CCATGAGCAC AACCCTCCGC 
TTGGGAAAAC AGATCCTCGA ATCCATAGAA GCCATTCACT CCGTTGGCTT TCTCCATCGA 
GACATCAAAC CGTCAAATTT TGCCATGGGA AGGCTGCCTT CAACATTCCG GAAATGTTAT 
ATGCTAGATT TTGGGCTCGC AAGACAGTAT ACAAACACCA ATGGAGAAGT CAGACCGACT 
TTCATTCCTA GAACTGTTGC AGGATTTCGA GGGACTGTTA GATATGCATC TGTGAATGCA 
CATAAAAACA AAGAAATGGG TCGCCATGAT GACTTATGGT CATTGTTTTA CATGCTGGTT 
GAGTTTGCTG CTGGGCAGCT GCCATGGAGG AAAATCAAGG ACAAGGAGCA AGTGGGTCAG 
ATTAAGGAGC GCTACGATCA TAGGATGCTT CTTAAACACA TGCCGTCAGA GTTTAATGTC 
TTCCTGGAGC ATGTTTTAGC CCTTGACTAC TATACCAAAC CAGACTATCA GCTCCTGATG 
TCAGTGTTCG AGAACAGCAT GAAGGAACGC ATCATCACTG AAAACGAGCC CTATGATTGG 
GAGAAAAGTG GGACGGAGAC TATTATCTCT GCTACCACAC CGACAGCAGC TCAGCAGAAC 
ACGAGACCCA CAGCCGCGAT GGTCGGGGTT ATAAATGTGA CCCCGATGCC AGGTGACCTT 
CCAAGGGAAA ACACAGATGA TGTTGTACAG GATGAGCACC TTAGTGACCA GGAAAACGCC 
CCTCCAATTC CCTCTGGACG GCCAGTTGAT GGCAGCCAGA TGCCCACAGA TGCAGACGGA 
TGGGAGGAGA CAGACTTCAA CCGCAACAAA CTCAGGATTA ACCTTGGAAA GGGAGGTCAA 
GAAGAGGACG GTAGCCGGGG TGTGTGTCCT GTTTCGCCAC AAAGGGGAGG TTTGGACTCT 
CCAGGGATGC AGGTTCACTC GATCAGGTAC CGACGGGTCA ACAGTCCTGA ATCTGATCGC 
ATGTCTGCTG CTGATGGACG GGACGGTTAC GGCCAACGCT CTCGAATGGA CATTCTGGGT 
TCTCCATCTC GTCACGTTTA CTCCTCTCAG CCCGCTCAGA TGCTCTCGGT GGACGGTTGC 
AGAGGCCGTA ATGAAGCCTC TGCATCGGTG GACCAGGAAG CCCACAGCAA TGCCTTCATC 
CGCTCAGTCC CGCTCGCAGA AGAAGAGGAC TTCGACAGCA AAGAGTGGGT GATCATAGAC 
AAGGAAATGG AGCTCAGAGA CTTCCACCAC CTTCCAGGAG CCGAACCTAC CACTTCCGGC 
ACGACGGATG AGGAGCCGGA GGAATTACGC CCTCTGGAGG AAGGAGATGA TCGGAGGAGA 
CACAGAGGGA ACGATCCATC CGTCAGAACC AAAACGTCCC ATGGAGAACG GACTACACGA 
GGGATGCTAC CACTGACGGA GGAAGAGACA TCCAGGAGGA GTGGGAAAGG CCAGTCCACG 
TCAATGGAGA GCCCTGCGCA GTCACCCTGT CACTCGCTGC CCTCCGGACG GCCCCGGCGG 
AGAGATGCAG ATAACAACGG ACCTCAGAGA CAGGCCCCAA CCCCAAGTGA GCCTCTATTA 
TCACCCACTG ACTACAGGAC TAAATCCCCA TCCTCTGAGA AGGAGATGTT CCATATACTT 
CCAAAGCTGC AGGCTAAGAG AGCCGACTTC CTTACAGTCA TGCTTACAGG AAGCCTTCAC 
CAGCGCCGGG CCTTCACAGC CACACCGCCT GAGACACAAG ATGAGGGGCC AAGAGATGAT 
GACGTCACAA AGAGCGAATC AAGCAGCGGA GCAAGTGAAA AGAGTACTGA ACGTAGTCAA 
GATGGGGCTC CATCTACCTT GGTTGCCGAT GATCCACAAA AAGAGTCAAA AGAACCAGGT 
TCGGTTGCTG ATGTAGATCC TGACCAAGAA GATGTCTCAA ACACCCTTGT TTTGTTTTCA 
CCAGGAGACA CGAAGAAGTC ACCAAATGCT ATAATCAGGG CTGAAGTTGA TCAGGGTGTG 
GGGAGTTTTG ATGCCCCTCA AAATGAAAAT CAAGGTCAAT CAGAGGTCCA GAAACAAGAG 
GACATCAAGC AAGCAGAGAG ACCTATAGAT GGAAGGCAAG CCGATGTAAC TGGGTTTGAC 
CCATTCATAC AGTCATCTAA ACCAGTGGTA ACAATCTCAA CAACTCCAGT GGCCTCTGCC 
TCAATTGCAT CACCCCTTTT CTCCAAAGTG GAGCAAACTT TTGTACACAT TGCAGAGACA 
ACCCATCTGA ATGTTATGTC TTCCAGTAGT GGACAGGTTC TGGAAGTTTT ACAGGAGGTT 
GAAGGCAAAT TACAGTCCAA ACTGACAAAA GACAAACCAG AGCCAGGAGG AGGTGCAGCT 
TGTGTCCAGT CTGAAGTCAT CTCTCAGATT CTTACAGAGG AATCTCAGGT ACCTAATGTC 
CTAGATGGGT CTTTGCCAAA TGAATCAACT GTAAAGCAAG AAAATCCTAC GGATCCTCTA 
TGTGTACAGT CTATGGAACC CATCATTCAA GATTCAACAA TTGACTCCAA TCAGGACGAA 
AAACCAAAAG AAGAAATTCA AGAGGGGAGC ACAGTACCTG ATATTGTTTC TAAACCTCCA 
AGGCCAAGAA GCAGAAGTCG GATTCCTATT CTAGTCCCGG AAGAGGAGCC AGGATCTGAG 
AAATCCCTGT CCACCAAAGA ACGAATGCGT AGAAGAGTCA TGCAGCAAGA CCTGGCCCGT 
CAAGTTGTGG AAAGACATCG TCAGGGTCGC TGGATAAGAC TGAATTCCAG GACATCATCT 
TCGTTATCTT CTGGAGATGA GCCCAAGAGA CCTTCAGAGA CTCTTTCTAC TACAGGCTCG 
GAAGAAGATG TTCTTGAATC TGATGATTCC CTAAACAAGC TCAAGAAGGA GGCTGAGGAA 
AAGCACCAAA CTGGCTGGCA CAGCAGGATC CCTCGACCAG TCACCCCAAT AAGGAAGCCT 
TCGACTAAAC TAGTTGTTGC ACCTCCTTTG AGCCAACCTG AAGCTGCAAA ATTAGAACAA 
AAAGCAAGCA ATGGCCCAAA AGTACAGTCA CAATCAGGAT CAAGTGCTCC AGCATTTCAT 
GCAAGATCAA AATCAACTCT GTCCCATTCG ACCATGCCAA GTGGAAGCAA TTGCATGCCT 
CTCTCCTGTA TTCCTGCAGC ATCCCGCCGC AGCTTAAGCA CCGCAAACTG CACTTTCTCT 
TCAGCCCGTA TAGCTAGTCC CTCGCCCCAT AGGCTACACT TGCGAACTGC TGTTATTGAA 
TCCCGCAAGC AACCGTTTGC CCTCCGAACC ACTGTGGAGC TCCCATTGAA GTCTAGGACT 
ACTACTTGCA GCAATCCTGC AAAGGACACC AAACCATCAC CAAACAAAGC CACCAACACA 
ACTGTCAGGA ATCGAACCAA AGCTTCTACT CAATCAAAAA ACACAGTTCC CGCTAAAAAG 
GAAAAAAGCA CAAGCAACAA TGCAGCTGCA GCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304406.1
CDS	314..4132
Translation	MNGTAEQQDILPPHCMVKDRWKVLKKIGGGGFGEIYEALDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKNHVCKFIGCGRNDKFNYVVMQLQGRNLADLRRSQPRGTFTMSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTFRKCYMLDFGLARQYTNTNGEVRPTFIPRTVAGFRGTVRYASVNAHKNKEMGRHDDLWSLFYMLVEFAAGQLPWRKIKDKEQVGQIKERYDHRMLLKHMPSEFNVFLEHVLALDYYTKPDYQLLMSVFENSMKERIITENEPYDWEKSGTETIISATTPTAAQQNTRPTAAMVGVINVTPMPGDLPRENTDDVVQDEHLSDQENAPPIPSGRPVDGSQMPTDADGWEETDFNRNKLRINLGKGGQEEDGSRGVCPVSPQRGGLDSPGMQVHSIRYRRVNSPESDRMSAADGRDGYGQRSRMDILGSPSRHVYSSQPAQMLSVDGCRGRNEASASVDQEAHSNAFIRSVPLAEEEDFDSKEWVIIDKEMELRDFHHLPGAEPTTSGTTDEEPEELRPLEEGDDRRRHRGNDPSVRTKTSHGERTTRGMLPLTEEETSRRSGKGQSTSMESPAQSPCHSLPSGRPRRRDADNNGPQRQAPTPSEPLLSPTDYRTKSPSSEKEMFHILPKLQAKRADFLTVMLTGSLHQRRAFTATPPETQDEGPRDDDVTKSESSSGASEKSTERSQDGAPSTLVADDPQKESKEPGSVADVDPDQEDVSNTLVLFSPGDTKKSPNAIIRAEVDQGVGSFDAPQNENQGQSEVQKQEDIKQAERPIDGRQADVTGFDPFIQSSKPVVTISTTPVASASIASPLFSKVEQTFVHIAETTHLNVMSSSSGQVLEVLQEVEGKLQSKLTKDKPEPGGGAACVQSEVISQILTEESQVPNVLDGSLPNESTVKQENPTDPLCVQSMEPIIQDSTIDSNQDEKPKEEIQEGSTVPDIVSKPPRPRSRSRIPILVPEEEPGSEKSLSTKERMRRRVMQQDLARQVVERHRQGRWIRLNSRTSSSLSSGDEPKRPSETLSTTGSEEDVLESDDSLNKLKKEAEEKHQTGWHSRIPRPVTPIRKPSTKLVVAPPLSQPEAAKLEQKASNGPKVQSQSGSSAPAFHARSKSTLSHSTMPSGSNCMPLSCIPAASRRSLSTANCTFSSARIASPSPHRLHLRTAVIESRKQPFALRTTVELPLKSRTTTCSNPAKDTKPSPNKATNTTVRNRTKASTQSKNTVPAKKEKSTSNNAAAAR

Target ORF information:

RefSeq Version	XM_009306131.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tau tubulin kinase 1a (ttbk1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306131.2

ORF Insert Sequence:

ATGAACGGCA CTGCAGAGCA GCAGGACATT CTGCCCCCTC ATTGCATGGT CAAAGATCGA 
TGGAAAGTGC TGAAGAAGAT CGGAGGAGGA GGGTTTGGGG AAATCTACGA GGCGCTGGAC 
CTGCTGACTC GAGAGAATGT GGCGTTAAAA GTGGAGTCAG CGCAACAGCC CAAACAAGTC 
CTGAAGATGG AGGTAGCAGT TTTGAAGAAA CTGCAGGGTA AAAACCACGT ATGTAAGTTC 
ATAGGCTGTG GGCGAAACGA TAAATTCAAC TATGTCGTCA TGCAGCTGCA GGGCAGAAAC 
CTGGCAGATC TCAGGCGAAG TCAGCCTCGC GGCACCTTTA CCATGAGCAC AACCCTCCGC 
TTGGGAAAAC AGATCCTCGA ATCCATAGAA GCCATTCACT CCGTTGGCTT TCTCCATCGA 
GACATCAAAC CGTCAAATTT TGCCATGGGA AGGCTGCCTT CAACATTCCG GAAATGTTAT 
ATGCTAGATT TTGGGCTCGC AAGACAGTAT ACAAACACCA ATGGAGAAGT CAGACCGACT 
TTCATTCCTA GAACTGTTGC AGGATTTCGA GGGACTGTTA GATATGCATC TGTGAATGCA 
CATAAAAACA AAGAAATGGG TCGCCATGAT GACTTATGGT CATTGTTTTA CATGCTGGTT 
GAGTTTGCTG CTGGGCAGCT GCCATGGAGG AAAATCAAGG ACAAGGAGCA AGTGGGTCAG 
ATTAAGGAGC GCTACGATCA TAGGATGCTT CTTAAACACA TGCCGTCAGA GTTTAATGTC 
TTCCTGGAGC ATGTTTTAGC CCTTGACTAC TATACCAAAC CAGACTATCA GCTCCTGATG 
TCAGTGTTCG AGAACAGCAT GAAGGAACGC ATCATCACTG AAAACGAGCC CTATGATTGG 
GAGAAAAGTG GGACGGAGAC TATTATCTCT GCTACCACAC CGACAGCAGC TCAGCAGAAC 
ACGAGACCCA CAGCCGCGAT GGTCGGGGTT ATAAATGTGA CCCCGATGCC AGGTGACCTT 
CCAAGGGAAA ACACAGATGA TGTTGTACAG GATGAGCACC TTAGTGACCA GGAAAACGCC 
CCTCCAATTC CCTCTGGACG GCCAGTTGAT GGCAGCCAGA TGCCCACAGA TGCAGACGGA 
TGGGAGGAGA CAGACTTCAA CCGCAACAAA CTCAGGATTA ACCTTGGAAA GGGAGGTCAA 
GAAGAGGACG GTAGCCGGGG TGTGTGTCCT GTTTCGCCAC AAAGGGGAGG TTTGGACTCT 
CCAGGGATGC AGGTTCACTC GATCAGGTAC CGACGGGTCA ACAGTCCTGA ATCTGATCGC 
ATGTCTGCTG CTGATGGACG GGACGGTTAC GGCCAACGCT CTCGAATGGA CATTCTGGGT 
TCTCCATCTC GTCACGTTTA CTCCTCTCAG CCCGCTCAGA TGCTCTCGGT GGACGGTTGC 
AGAGGCCGTA ATGAAGCCTC TGCATCGGTG GACCAGGAAG CCCACAGCAA TGCCTTCATC 
CGCTCAGTCC CGCTCGCAGA AGAAGAGGAC TTCGACAGCA AAGAGTGGGT GATCATAGAC 
AAGGAAATGG AGCTCAGAGA CTTCCACCAC CTTCCAGGAG CCGAACCTAC CACTTCCGGC 
ACGACGGATG AGGAGCCGGA GGAATTACGC CCTCTGGAGG AAGGAGATGA TCGGAGGAGA 
CACAGAGGGA ACGATCCATC CGTCAGAACC AAAACGTCCC ATGGAGAACG GACTACACGA 
GGGATGCTAC CACTGACGGA GGAAGAGACA TCCAGGAGGA GTGGGAAAGG CCAGTCCACG 
TCAATGGAGA GCCCTGCGCA GTCACCCTGT CACTCGCTGC CCTCCGGACG GCCCCGGCGG 
AGAGATGCAG ATAACAACGG ACCTCAGAGA CAGGCCCCAA CCCCAAGTGA GCCTCTATTA 
TCACCCACTG ACTACAGGAC TAAATCCCCA TCCTCTGAGA AGGAGATGTT CCATATACTT 
CCAAAGCTGC AGGCTAAGAG AGCCGACTTC CTTACAGTCA TGCTTACAGG AAGCCTTCAC 
CAGCGCCGGG CCTTCACAGC CACACCGCCT GAGACACAAG ATGAGGGGCC AAGAGATGAT 
GACGTCACAA AGAGCGAATC AAGCAGCGGA GCAAGTGAAA AGAGTACTGA ACGTAGTCAA 
GATGGGGCTC CATCTACCTT GGTTGCCGAT GATCCACAAA AAGAGTCAAA AGAACCAGGT 
TCGGTTGCTG ATGTAGATCC TGACCAAGAA GATGTCTCAA ACACCCTTGT TTTGTTTTCA 
CCAGGAGACA CGAAGAAGTC ACCAAATGCT ATAATCAGGG CTGAAGTTGA TCAGGGTGTG 
GGGAGTTTTG ATGCCCCTCA AAATGAAAAT CAAGGTCAAT CAGAGGTCCA GAAACAAGAG 
GACATCAAGC AAGCAGAGAG ACCTATAGAT GGAAGGCAAG CCGATGTAAC TGGGTTTGAC 
CCATTCATAC AGTCATCTAA ACCAGTGGTA ACAATCTCAA CAACTCCAGT GGCCTCTGCC 
TCAATTGCAT CACCCCTTTT CTCCAAAGTG GAGCAAACTT TTGTACACAT TGCAGAGACA 
ACCCATCTGA ATGTTATGTC TTCCAGTAGT GGACAGGTTC TGGAAGTTTT ACAGGAGGTT 
GAAGGCAAAT TACAGTCCAA ACTGACAAAA GACAAACCAG AGCCAGGAGG AGGTGCAGCT 
TGTGTCCAGT CTGAAGTCAT CTCTCAGATT CTTACAGAGG AATCTCAGGT ACCTAATGTC 
CTAGATGGGT CTTTGCCAAA TGAATCAACT GTAAAGCAAG AAAATCCTAC GGATCCTCTA 
TGTGTACAGT CTATGGAACC CATCATTCAA GATTCAACAA TTGACTCCAA TCAGGACGAA 
AAACCAAAAG AAGAAATTCA AGAGGGGAGC ACAGTACCTG ATATTGTTTC TAAACCTCCA 
AGGCCAAGAA GCAGAAGTCG GATTCCTATT CTAGTCCCGG AAGAGGAGCC AGGATCTGAG 
AAATCCCTGT CCACCAAAGA ACGAATGCGT AGAAGAGTCA TGCAGCAAGA CCTGGCCCGT 
CAAGTTGTGG AAAGACATCG TCAGGGTCGC TGGATAAGAC TGAATTCCAG GACATCATCT 
TCGTTATCTT CTGGAGATGA GCCCAAGAGA CCTTCAGAGA CTCTTTCTAC TACAGGCTCG 
GAAGAAGATG TTCTTGAATC TGATGATTCC CTAAACAAGC TCAAGAAGGA GGCTGAGGAA 
AAGCACCAAA CTGGCTGGCA CAGCAGGATC CCTCGACCAG TCACCCCAAT AAGGAAGCCT 
TCGACTAAAC TAGTTGTTGC ACCTCCTTTG AGCCAACCTG AAGCTGCAAA ATTAGAACAA 
AAAGCAAGCA ATGGCCCAAA AGTACAGTCA CAATCAGGAT CAAGTGCTCC AGCATTTCAT 
GCAAGATCAA AATCAACTCT GTCCCATTCG ACCATGCCAA GTGGAAGCAA TTGCATGCCT 
CTCTCCTGTA TTCCTGCAGC ATCCCGCCGC AGCTTAAGCA CCGCAAACTG CACTTTCTCT 
TCAGCCCGTA TAGCTAGTCC CTCGCCCCAT AGGCTACACT TGCGAACTGC TGTTATTGAA 
TCCCGCAAGC AACCGTTTGC CCTCCGAACC ACTGTGGAGC TCCCATTGAA GTCTAGGACT 
ACTACTTGCA GCAATCCTGC AAAGGACACC AAACCATCAC CAAACAAAGC CACCAACACA 
ACTGTCAGGA ATCGAACCAA AGCTTCTACT CAATCAAAAA ACACAGTTCC CGCTAAAAAG 
GAAAAAAGCA CAAGCAACAA TGCAGCTGCA GCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TTBK1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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