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AGPAT2 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol AGPAT2
Entrez Gene ID 10555
Full Name 1-acylglycerol-3-phosphate O-acyltransferase 2
Synonyms 1-AGPAT2,BSCL,BSCL1,LPAAB,LPAAT-beta
General protein information
Preferred Names

1-acylglycerol-3-phosphate O-acyltransferase 2

Names

1-acyl-sn-glycerol-3-phosphate acyltransferase beta
1-AGP acyltransferase 2
1-AGPAT 2
1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta)
lysophosphatidic acid acyltransferase-beta
testicular tissue protein Li 143
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

9

9q34.3
Summary This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. The protein is located within the endoplasmic reticulum membrane and converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. Mutations in this gene have been associated with congenital generalized lipodystrophy (CGL), or Berardinelli-Seip syndrome, a disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008].
Disorder MIM:

603100

Disorder Html: Lipodystrophy, congenital generalized, type 1, 608594 (3)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001012727.1 NP_001012745.1 1-acyl-sn-glycerol-3-phosphate acyltransferase beta isoform b precursor
NM_006412.3 NP_006403.2 1-acyl-sn-glycerol-3-phosphate acyltransferase beta isoform a precursor
NM_006412.4 NP_006403.2 1-acyl-sn-glycerol-3-phosphate acyltransferase beta isoform a precursor
NM_001012727.2 NP_001012745.1 1-acyl-sn-glycerol-3-phosphate acyltransferase beta isoform b precursor

Pathways from BioSystems


Homologies


AGPAT2 XP_001094500.1
Bos taurus (cattle) AGPAT2 NP_001073733.1
Rattus norvegicus (Norway rat) Agpat2 NP_001101291.1
Homo sapiens (human) AGPAT2 NP_006403.2
Gallus gallus (chicken) AGPAT2 XP_001235300.2
Xenopus tropicalis (tropical clawed frog) agpat2 NP_001123741.1
Pan troglodytes (chimpanzee) AGPAT2 XP_001162511.3
Mus musculus (house mouse) Agpat2 NP_080488.1
Canis lupus familiaris (dog) AGPAT2 XP_548370.4

Gene Ontology


Bibliography

Related articles in PubMed

Silencing LPAAT? inhibits tumor growth of cisplatin-resistant human osteosarcoma in vivo and in vitro.
Song L, Duan P, Gan Y, Li P, Zhao C, Xu J, Zhang Z, Zhou Q
International journal of oncology50(2)535-544(2017 Feb)

Divergent metabolic phenotype between two sisters with congenital generalized lipodystrophy due to double AGPAT2 homozygous mutations. a clinical, genetic and in silico study.
Cort?s VA, Smalley SV, Goldenberg D, Lagos CF, Hodgson MI, Santos JL
PloS one9(1)e87173(2014)

MicroRNA-24 inhibits osteosarcoma cell proliferation both in vitro and in vivo by targeting LPAAT?.
Song L, Yang J, Duan P, Xu J, Luo X, Luo F, Zhang Z, Hou T, Liu B, Zhou Q
Archives of biochemistry and biophysics535(2)128-35(2013 Jul)

Lysophosphatidic acid acyltransferase beta regulates mTOR signaling.
Blaskovich MA, Yendluri V, Lawrence HR, Lawrence NJ, Sebti SM, Springett GM
PloS one8(10)e78632(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that LPAATbeta had high expression in osteosarcoma patients who received cisplatin treatment and cisplatin-resistant cancer cell lines. In vitro and in vivo studies provide evidence that LPAATB plays an important role in osteosarcoma.
Title: Silencing LPAAT? inhibits tumor growth of cisplatin-resistant human osteosarcoma in vivo and in vitro.

Data show that missense mutation c.299G>A changes serine in the acyltransferase NHX4D motif of AGPAT2, and intronic c.493-1G>C mutation destroy a splicing site that leads to exon 4 skipping and deletion of whole AGPAT2 substrate binding domain.
Title: Divergent metabolic phenotype between two sisters with congenital generalized lipodystrophy due to double AGPAT2 homozygous mutations. a clinical, genetic and in silico study.

miR-24 may play an important role in inhibiting osteosarcoma growth through suppression of LPAATbeta.
Title: MicroRNA-24 inhibits osteosarcoma cell proliferation both in vitro and in vivo by targeting LPAAT?.

the ability of LPAAT-beta to regulate mTOR function
Title: Lysophosphatidic acid acyltransferase beta regulates mTOR signaling.

novel nonsense a missense mutations were found in two patients with congenital generalized lipodystrophy type 1.
Title: Identification of a novel nonsense mutation and a missense substitution in the AGPAT2 gene causing congenital generalized lipodystrophy type 1.

The following AGPAT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGPAT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu22397 NM_006412.4
Latest version!		 Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu23150 NM_001012727.2
Latest version!		 Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
View Old Accession Versions >>
OHu22397 NM_006412.3 Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu23150 NM_001012727.1 Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu22397
Clone ID Related Accession (Same CDS sequence) NM_006412.3 , NM_006412.4
Accession Version NM_006412.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 837bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-01
Organism Homo sapiens(human)
Product 1-acyl-sn-glycerol-3-phosphate acyltransferase beta isoform a precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF347518.1, AL590226.23, AF000237.1 and BC000026.2. This sequence is a reference standard in the RefSeqGene project. On Jul 5, 2005 this sequence version replaced NM_006412.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF011374.1, SRR1163655.83915.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153980, SAMEA2154125 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGAGCTGT GGCCGTGTCT GGCCGCGGCG CTGCTGTTGC TGCTGCTGCT GGTGCAGCTG 
AGCCGCGCGG CCGAGTTCTA CGCCAAGGTC GCCCTGTACT GCGCGCTGTG CTTCACGGTG 
TCCGCCGTGG CCTCGCTCGT CTGCCTGCTG CGCCACGGCG GCCGGACGGT GGAGAACATG 
AGCATCATCG GCTGGTTCGT GCGAAGCTTC AAGTACTTTT ACGGGCTCCG CTTCGAGGTG 
CGGGACCCGC GCAGGCTGCA GGAGGCCCGT CCCTGTGTCA TCGTCTCCAA CCACCAGAGC 
ATCCTGGACA TGATGGGCCT CATGGAGGTC CTTCCGGAGC GCTGCGTGCA GATCGCCAAG 
CGGGAGCTGC TCTTCCTGGG GCCCGTGGGC CTCATCATGT ACCTCGGGGG CGTCTTCTTC 
ATCAACCGGC AGCGCTCTAG CACTGCCATG ACAGTGATGG CCGACCTGGG CGAGCGCATG 
GTCAGGGAGA ACCTCAAAGT GTGGATCTAT CCCGAGGGTA CTCGCAACGA CAATGGGGAC 
CTGCTGCCTT TTAAGAAGGG CGCCTTCTAC CTGGCAGTCC AGGCACAGGT GCCCATCGTC 
CCCGTGGTGT ACTCTTCCTT CTCCTCCTTC TACAACACCA AGAAGAAGTT CTTCACTTCA 
GGAACAGTCA CAGTGCAGGT GCTGGAAGCC ATCCCCACCA GCGGCCTCAC TGCGGCGGAC 
GTCCCTGCGC TCGTGGACAC CTGCCACCGG GCCATGAGGA CCACCTTCCT CCACATCTCC 
AAGACCCCCC AGGAGAACGG GGCCACTGCG GGGTCTGGCG TGCAGCCGGC CCAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006403.2
CDS103..939
Translation

Target ORF information:

RefSeq Version NM_006412.3
Organism Homo sapiens(human)
Definition Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_006412.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGAGCTGT GGCCGTGTCT GGCCGCGGCG CTGCTGTTGC TGCTGCTGCT GGTGCAGCTG 
AGCCGCGCGG CCGAGTTCTA CGCCAAGGTC GCCCTGTACT GCGCGCTGTG CTTCACGGTG 
TCCGCCGTGG CCTCGCTCGT CTGCCTGCTG CGCCACGGCG GCCGGACGGT GGAGAACATG 
AGCATCATCG GCTGGTTCGT GCGAAGCTTC AAGTACTTTT ACGGGCTCCG CTTCGAGGTG 
CGGGACCCGC GCAGGCTGCA GGAGGCCCGT CCCTGTGTCA TCGTCTCCAA CCACCAGAGC 
ATCCTGGACA TGATGGGCCT CATGGAGGTC CTTCCGGAGC GCTGCGTGCA GATCGCCAAG 
CGGGAGCTGC TCTTCCTGGG GCCCGTGGGC CTCATCATGT ACCTCGGGGG CGTCTTCTTC 
ATCAACCGGC AGCGCTCTAG CACTGCCATG ACAGTGATGG CCGACCTGGG CGAGCGCATG 
GTCAGGGAGA ACCTCAAAGT GTGGATCTAT CCCGAGGGTA CTCGCAACGA CAATGGGGAC 
CTGCTGCCTT TTAAGAAGGG CGCCTTCTAC CTGGCAGTCC AGGCACAGGT GCCCATCGTC 
CCCGTGGTGT ACTCTTCCTT CTCCTCCTTC TACAACACCA AGAAGAAGTT CTTCACTTCA 
GGAACAGTCA CAGTGCAGGT GCTGGAAGCC ATCCCCACCA GCGGCCTCAC TGCGGCGGAC 
GTCCCTGCGC TCGTGGACAC CTGCCACCGG GCCATGAGGA CCACCTTCCT CCACATCTCC 
AAGACCCCCC AGGAGAACGG GGCCACTGCG GGGTCTGGCG TGCAGCCGGC CCAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22397
Clone ID Related Accession (Same CDS sequence) NM_006412.3 , NM_006412.4
Accession Version NM_006412.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 837bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-30
Organism Homo sapiens(human)
Product 1-acyl-sn-glycerol-3-phosphate acyltransferase beta isoform a precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF347518.1, AL590226.23, AF000237.1 and BC000026.2. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_006412.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.485265.1, SRR3476690.542634.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153980, SAMEA2154125 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000371696.7/ ENSP00000360761.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGAGCTGT GGCCGTGTCT GGCCGCGGCG CTGCTGTTGC TGCTGCTGCT GGTGCAGCTG 
AGCCGCGCGG CCGAGTTCTA CGCCAAGGTC GCCCTGTACT GCGCGCTGTG CTTCACGGTG 
TCCGCCGTGG CCTCGCTCGT CTGCCTGCTG CGCCACGGCG GCCGGACGGT GGAGAACATG 
AGCATCATCG GCTGGTTCGT GCGAAGCTTC AAGTACTTTT ACGGGCTCCG CTTCGAGGTG 
CGGGACCCGC GCAGGCTGCA GGAGGCCCGT CCCTGTGTCA TCGTCTCCAA CCACCAGAGC 
ATCCTGGACA TGATGGGCCT CATGGAGGTC CTTCCGGAGC GCTGCGTGCA GATCGCCAAG 
CGGGAGCTGC TCTTCCTGGG GCCCGTGGGC CTCATCATGT ACCTCGGGGG CGTCTTCTTC 
ATCAACCGGC AGCGCTCTAG CACTGCCATG ACAGTGATGG CCGACCTGGG CGAGCGCATG 
GTCAGGGAGA ACCTCAAAGT GTGGATCTAT CCCGAGGGTA CTCGCAACGA CAATGGGGAC 
CTGCTGCCTT TTAAGAAGGG CGCCTTCTAC CTGGCAGTCC AGGCACAGGT GCCCATCGTC 
CCCGTGGTGT ACTCTTCCTT CTCCTCCTTC TACAACACCA AGAAGAAGTT CTTCACTTCA 
GGAACAGTCA CAGTGCAGGT GCTGGAAGCC ATCCCCACCA GCGGCCTCAC TGCGGCGGAC 
GTCCCTGCGC TCGTGGACAC CTGCCACCGG GCCATGAGGA CCACCTTCCT CCACATCTCC 
AAGACCCCCC AGGAGAACGG GGCCACTGCG GGGTCTGGCG TGCAGCCGGC CCAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006403.2
CDS101..937
Translation

Target ORF information:

RefSeq Version NM_006412.4
Organism Homo sapiens(human)
Definition Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_006412.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGAGCTGT GGCCGTGTCT GGCCGCGGCG CTGCTGTTGC TGCTGCTGCT GGTGCAGCTG 
AGCCGCGCGG CCGAGTTCTA CGCCAAGGTC GCCCTGTACT GCGCGCTGTG CTTCACGGTG 
TCCGCCGTGG CCTCGCTCGT CTGCCTGCTG CGCCACGGCG GCCGGACGGT GGAGAACATG 
AGCATCATCG GCTGGTTCGT GCGAAGCTTC AAGTACTTTT ACGGGCTCCG CTTCGAGGTG 
CGGGACCCGC GCAGGCTGCA GGAGGCCCGT CCCTGTGTCA TCGTCTCCAA CCACCAGAGC 
ATCCTGGACA TGATGGGCCT CATGGAGGTC CTTCCGGAGC GCTGCGTGCA GATCGCCAAG 
CGGGAGCTGC TCTTCCTGGG GCCCGTGGGC CTCATCATGT ACCTCGGGGG CGTCTTCTTC 
ATCAACCGGC AGCGCTCTAG CACTGCCATG ACAGTGATGG CCGACCTGGG CGAGCGCATG 
GTCAGGGAGA ACCTCAAAGT GTGGATCTAT CCCGAGGGTA CTCGCAACGA CAATGGGGAC 
CTGCTGCCTT TTAAGAAGGG CGCCTTCTAC CTGGCAGTCC AGGCACAGGT GCCCATCGTC 
CCCGTGGTGT ACTCTTCCTT CTCCTCCTTC TACAACACCA AGAAGAAGTT CTTCACTTCA 
GGAACAGTCA CAGTGCAGGT GCTGGAAGCC ATCCCCACCA GCGGCCTCAC TGCGGCGGAC 
GTCCCTGCGC TCGTGGACAC CTGCCACCGG GCCATGAGGA CCACCTTCCT CCACATCTCC 
AAGACCCCCC AGGAGAACGG GGCCACTGCG GGGTCTGGCG TGCAGCCGGC CCAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23150
Clone ID Related Accession (Same CDS sequence) NM_001012727.1 , NM_001012727.2
Accession Version NM_001012727.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 741bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-16
Organism Homo sapiens(human)
Product 1-acyl-sn-glycerol-3-phosphate acyltransferase beta isoform b precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF347518.1, CN407343.1, BC004529.2, AL590226.23 and BC000026.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC004529.2, SRR1163655.577851.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGCTGT GGCCGTGTCT GGCCGCGGCG CTGCTGTTGC TGCTGCTGCT GGTGCAGCTG 
AGCCGCGCGG CCGAGTTCTA CGCCAAGGTC GCCCTGTACT GCGCGCTGTG CTTCACGGTG 
TCCGCCGTGG CCTCGCTCGT CTGCCTGCTG CGCCACGGCG GCCGGACGGT GGAGAACATG 
AGCATCATCG GCTGGTTCGT GCGAAGCTTC AAGTACTTTT ACGGGCTCCG CTTCGAGGTG 
CGGGACCCGC GCAGGCTGCA GGAGGCCCGT CCCTGTGTCA TCGTCTCCAA CCACCAGAGC 
ATCCTGGACA TGATGGGCCT CATGGAGGTC CTTCCGGAGC GCTGCGTGCA GATCGCCAAG 
CGGGAGCTGC TCTTCCTGGG GCCCGTGGGC CTCATCATGT ACCTCGGGGG CGTCTTCTTC 
ATCAACCGGC AGCGCTCTAG CACTGCCATG ACAGTGATGG CCGACCTGGG CGAGCGCATG 
GTCAGGGAGA ACGTGCCCAT CGTCCCCGTG GTGTACTCTT CCTTCTCCTC CTTCTACAAC 
ACCAAGAAGA AGTTCTTCAC TTCAGGAACA GTCACAGTGC AGGTGCTGGA AGCCATCCCC 
ACCAGCGGCC TCACTGCGGC GGACGTCCCT GCGCTCGTGG ACACCTGCCA CCGGGCCATG 
AGGACCACCT TCCTCCACAT CTCCAAGACC CCCCAGGAGA ACGGGGCCAC TGCGGGGTCT 
GGCGTGCAGC CGGCCCAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001012745.1
CDS103..843
Translation

Target ORF information:

RefSeq Version NM_001012727.1
Organism Homo sapiens(human)
Definition Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001012727.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGCTGT GGCCGTGTCT GGCCGCGGCG CTGCTGTTGC TGCTGCTGCT GGTGCAGCTG 
AGCCGCGCGG CCGAGTTCTA CGCCAAGGTC GCCCTGTACT GCGCGCTGTG CTTCACGGTG 
TCCGCCGTGG CCTCGCTCGT CTGCCTGCTG CGCCACGGCG GCCGGACGGT GGAGAACATG 
AGCATCATCG GCTGGTTCGT GCGAAGCTTC AAGTACTTTT ACGGGCTCCG CTTCGAGGTG 
CGGGACCCGC GCAGGCTGCA GGAGGCCCGT CCCTGTGTCA TCGTCTCCAA CCACCAGAGC 
ATCCTGGACA TGATGGGCCT CATGGAGGTC CTTCCGGAGC GCTGCGTGCA GATCGCCAAG 
CGGGAGCTGC TCTTCCTGGG GCCCGTGGGC CTCATCATGT ACCTCGGGGG CGTCTTCTTC 
ATCAACCGGC AGCGCTCTAG CACTGCCATG ACAGTGATGG CCGACCTGGG CGAGCGCATG 
GTCAGGGAGA ACGTGCCCAT CGTCCCCGTG GTGTACTCTT CCTTCTCCTC CTTCTACAAC 
ACCAAGAAGA AGTTCTTCAC TTCAGGAACA GTCACAGTGC AGGTGCTGGA AGCCATCCCC 
ACCAGCGGCC TCACTGCGGC GGACGTCCCT GCGCTCGTGG ACACCTGCCA CCGGGCCATG 
AGGACCACCT TCCTCCACAT CTCCAAGACC CCCCAGGAGA ACGGGGCCAC TGCGGGGTCT 
GGCGTGCAGC CGGCCCAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23150
Clone ID Related Accession (Same CDS sequence) NM_001012727.1 , NM_001012727.2
Accession Version NM_001012727.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 741bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-01
Organism Homo sapiens(human)
Product 1-acyl-sn-glycerol-3-phosphate acyltransferase beta isoform b precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF347518.1, CN407343.1, BC004529.2, AL590226.23 and BC000026.2. On May 31, 2019 this sequence version replaced NM_001012727.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC004529.2, DRR138522.939835.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGCTGT GGCCGTGTCT GGCCGCGGCG CTGCTGTTGC TGCTGCTGCT GGTGCAGCTG 
AGCCGCGCGG CCGAGTTCTA CGCCAAGGTC GCCCTGTACT GCGCGCTGTG CTTCACGGTG 
TCCGCCGTGG CCTCGCTCGT CTGCCTGCTG CGCCACGGCG GCCGGACGGT GGAGAACATG 
AGCATCATCG GCTGGTTCGT GCGAAGCTTC AAGTACTTTT ACGGGCTCCG CTTCGAGGTG 
CGGGACCCGC GCAGGCTGCA GGAGGCCCGT CCCTGTGTCA TCGTCTCCAA CCACCAGAGC 
ATCCTGGACA TGATGGGCCT CATGGAGGTC CTTCCGGAGC GCTGCGTGCA GATCGCCAAG 
CGGGAGCTGC TCTTCCTGGG GCCCGTGGGC CTCATCATGT ACCTCGGGGG CGTCTTCTTC 
ATCAACCGGC AGCGCTCTAG CACTGCCATG ACAGTGATGG CCGACCTGGG CGAGCGCATG 
GTCAGGGAGA ACGTGCCCAT CGTCCCCGTG GTGTACTCTT CCTTCTCCTC CTTCTACAAC 
ACCAAGAAGA AGTTCTTCAC TTCAGGAACA GTCACAGTGC AGGTGCTGGA AGCCATCCCC 
ACCAGCGGCC TCACTGCGGC GGACGTCCCT GCGCTCGTGG ACACCTGCCA CCGGGCCATG 
AGGACCACCT TCCTCCACAT CTCCAAGACC CCCCAGGAGA ACGGGGCCAC TGCGGGGTCT 
GGCGTGCAGC CGGCCCAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001012745.1
CDS101..841
Translation

Target ORF information:

RefSeq Version NM_001012727.2
Organism Homo sapiens(human)
Definition Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001012727.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGCTGT GGCCGTGTCT GGCCGCGGCG CTGCTGTTGC TGCTGCTGCT GGTGCAGCTG 
AGCCGCGCGG CCGAGTTCTA CGCCAAGGTC GCCCTGTACT GCGCGCTGTG CTTCACGGTG 
TCCGCCGTGG CCTCGCTCGT CTGCCTGCTG CGCCACGGCG GCCGGACGGT GGAGAACATG 
AGCATCATCG GCTGGTTCGT GCGAAGCTTC AAGTACTTTT ACGGGCTCCG CTTCGAGGTG 
CGGGACCCGC GCAGGCTGCA GGAGGCCCGT CCCTGTGTCA TCGTCTCCAA CCACCAGAGC 
ATCCTGGACA TGATGGGCCT CATGGAGGTC CTTCCGGAGC GCTGCGTGCA GATCGCCAAG 
CGGGAGCTGC TCTTCCTGGG GCCCGTGGGC CTCATCATGT ACCTCGGGGG CGTCTTCTTC 
ATCAACCGGC AGCGCTCTAG CACTGCCATG ACAGTGATGG CCGACCTGGG CGAGCGCATG 
GTCAGGGAGA ACGTGCCCAT CGTCCCCGTG GTGTACTCTT CCTTCTCCTC CTTCTACAAC 
ACCAAGAAGA AGTTCTTCAC TTCAGGAACA GTCACAGTGC AGGTGCTGGA AGCCATCCCC 
ACCAGCGGCC TCACTGCGGC GGACGTCCCT GCGCTCGTGG ACACCTGCCA CCGGGCCATG 
AGGACCACCT TCCTCCACAT CTCCAAGACC CCCCAGGAGA ACGGGGCCAC TGCGGGGTCT 
GGCGTGCAGC CGGCCCAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Silencing LPAAT? inhibits tumor growth of cisplatin-resistant human osteosarcoma in vivo and in vitro.
International journal of oncology50(2)535-544(2017 Feb)
Song L,Duan P,Gan Y,Li P,Zhao C,Xu J,Zhang Z,Zhou Q


book

Divergent metabolic phenotype between two sisters with congenital generalized lipodystrophy due to double AGPAT2 homozygous mutations. a clinical, genetic and in silico study.
PloS one9(1)e87173(2014)
Cort?s VA,Smalley SV,Goldenberg D,Lagos CF,Hodgson MI,Santos JL


book

MicroRNA-24 inhibits osteosarcoma cell proliferation both in vitro and in vivo by targeting LPAAT?.
Archives of biochemistry and biophysics535(2)128-35(2013 Jul)
Song L,Yang J,Duan P,Xu J,Luo X,Luo F,Zhang Z,Hou T,Liu B,Zhou Q


book

Lysophosphatidic acid acyltransferase beta regulates mTOR signaling.
PloS one8(10)e78632(2013)
Blaskovich MA,Yendluri V,Lawrence HR,Lawrence NJ,Sebti SM,Springett GM