MLANA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MLANA
Entrez Gene ID	2315
Full Name	melan-A
Synonyms	MART-1,MART1
General protein information	Preferred Names melan-A Names melanoma antigen recognized by T-cells 1 antigen LB39-AA antigen SK29-AA protein Melan-A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9p24.1
Disorder MIM:	605513

mRNA and Protein(s)

mRNA	Protein	Name
NM_005511.2	NP_005502.1	melanoma antigen recognized by T-cells 1
NM_005511.1	NP_005502.1	melanoma antigen recognized by T-cells 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MLANA	NP_005502.1
Mus musculus (house mouse)	Mlana	NP_084269.1
	MLANA	XP_001112920.1
Canis lupus familiaris (dog)	MLANA	NP_001181897.1
Rattus norvegicus (Norway rat)	Mlana	NP_001099818.1
Bos taurus (cattle)	MLANA	NP_001180064.1
Gallus gallus (chicken)	MLANA	XP_001232043.1
Pan troglodytes (chimpanzee)	MLANA	XP_001141417.1

Gene Ontology
Bibliography

Related articles in PubMed

Detection of Occult Invasion in Melanoma In Situ.
Bax MJ, Johnson TM, Harms PW, Schwartz JL, Zhao L, Fullen DR, Chan MP
JAMA dermatology152(11)1201-1208(2016 Nov)

Automated quantification of proliferation with automated hot-spot selection in phosphohistone H3/MART1 dual-stained stage I/II melanoma.
Nielsen PS, Riber-Hansen R, Schmidt H, Steiniche T
Diagnostic pathology1135(2016 Apr)

MelanA-negative spindle-cell associated melanoma, a distinct inflammatory phenotype correlated with dense infiltration of CD163 macrophages and loss of E-cadherin.
B?nnelykke-Behrndtz ML, Steiniche T, Damsgaard TE, Georgsen JB, Danielsen A, Bastholt L, M?ller HJ, N?rgaard PH, Schmidt H
Melanoma research25(2)113-8(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Melan-A-stained slides shows definitive invasion.
Title: Detection of Occult Invasion in Melanoma In Situ.

Report automated quantification of proliferation with automated hot-spot selection in phosphohistone H3/MART1 dual-stained stage I/II melanoma.
Title: Automated quantification of proliferation with automated hot-spot selection in phosphohistone H3/MART1 dual-stained stage I/II melanoma.

Knockdown of T-bet expression in Mart-127-35 -specific T-cell-receptor-engineered human CD4(+) CD25(-) and CD8(+) T cells attenuates effector function.
Title: Knockdown of T-bet expression in Mart-127-35 -specific T-cell-receptor-engineered human CD4(+) ?CD25(-) and CD8(+) T cells attenuates effector function.

Suggest MelanA-negative spindle-cell associated melanoma constitutes a distinct inflammatory phenotype correlated with dense infiltration of CD163 macrophages and loss of E-cadherin.
Title: MelanA-negative spindle-cell associated melanoma, a distinct inflammatory phenotype correlated with dense infiltration of CD163 macrophages and loss of E-cadherin.

Inhibin alpha-subunit, Melan A and MNF116 were not sensitive for pheochromocytomas.
Title: Inhibin alpha-subunit, Melan A and MNF116 in pheochromocytomas.

The following MLANA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MLANA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16236	NM_005511.2 Latest version!	Homo sapiens melan-A (MLANA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu16236	NM_005511.1	Homo sapiens melan-A (MLANA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MLANA ( NM_005511.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005502.1 Homo sapiens melan-A (MLANA), mRNA.

Price & Availability↑

CloneID	OHu16236
Clone ID Related Accession (Same CDS sequence)	NM_005511.2 , NM_005511.1
Accession Version	NM_005511.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	melanoma antigen recognized by T-cells 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL365360.9 and U06452.1. On May 17, 2019 this sequence version replaced NM_005511.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U06452.1, BF691815.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145893, SAMEA2147596 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000381477.8/ ENSP00000370886.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGCCAAGAG AAGATGCTCA CTTCATCTAT GGTTACCCCA AGAAGGGGCA CGGCCACTCT 
TACACCACGG CTGAAGAGGC CGCTGGGATC GGCATCCTGA CAGTGATCCT GGGAGTCTTA 
CTGCTCATCG GCTGTTGGTA TTGTAGAAGA CGAAATGGAT ACAGAGCCTT GATGGATAAA 
AGTCTTCATG TTGGCACTCA ATGTGCCTTA ACAAGAAGAT GCCCACAAGA AGGGTTTGAT 
CATCGGGACA GCAAAGTGTC TCTTCAAGAG AAAAACTGTG AACCTGTGGT TCCCAATGCT 
CCACCTGCTT ATGAGAAACT CTCTGCAGAA CAGTCACCAC CACCTTATTC ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005502.1
CDS	74..430
Translation	MPREDAHFIYGYPKKGHGHSYTTAEEAAGIGILTVILGVLLLIGCWYCRRRNGYRALMDKSLHVGTQCALTRRCPQEGFDHRDSKVSLQEKNCEPVVPNAPPAYEKLSAEQSPPPYSP

Target ORF information:

RefSeq Version	NM_005511.2
Organism	Homo sapiens(human)
Definition	Homo sapiens melan-A (MLANA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005511.2

ORF Insert Sequence:

ATGCCAAGAG AAGATGCTCA CTTCATCTAT GGTTACCCCA AGAAGGGGCA CGGCCACTCT 
TACACCACGG CTGAAGAGGC CGCTGGGATC GGCATCCTGA CAGTGATCCT GGGAGTCTTA 
CTGCTCATCG GCTGTTGGTA TTGTAGAAGA CGAAATGGAT ACAGAGCCTT GATGGATAAA 
AGTCTTCATG TTGGCACTCA ATGTGCCTTA ACAAGAAGAT GCCCACAAGA AGGGTTTGAT 
CATCGGGACA GCAAAGTGTC TCTTCAAGAG AAAAACTGTG AACCTGTGGT TCCCAATGCT 
CCACCTGCTT ATGAGAAACT CTCTGCAGAA CAGTCACCAC CACCTTATTC ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLANA ( NM_005511.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_005502.1 Homo sapiens melan-A (MLANA), mRNA.

Price & Availability↑

CloneID	OHu16236
Clone ID Related Accession (Same CDS sequence)	NM_005511.2 , NM_005511.1
Accession Version	NM_005511.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	melanoma antigen recognized by T-cells 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U06452.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U06452.1, BX444322.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145893, SAMEA2147596 [ECO:0000348] ##Evidence-Data-END##

ATGCCAAGAG AAGATGCTCA CTTCATCTAT GGTTACCCCA AGAAGGGGCA CGGCCACTCT 
TACACCACGG CTGAAGAGGC CGCTGGGATC GGCATCCTGA CAGTGATCCT GGGAGTCTTA 
CTGCTCATCG GCTGTTGGTA TTGTAGAAGA CGAAATGGAT ACAGAGCCTT GATGGATAAA 
AGTCTTCATG TTGGCACTCA ATGTGCCTTA ACAAGAAGAT GCCCACAAGA AGGGTTTGAT 
CATCGGGACA GCAAAGTGTC TCTTCAAGAG AAAAACTGTG AACCTGTGGT TCCCAATGCT 
CCACCTGCTT ATGAGAAACT CTCTGCAGAA CAGTCACCAC CACCTTATTC ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005502.1
CDS	54..410
Translation	MPREDAHFIYGYPKKGHGHSYTTAEEAAGIGILTVILGVLLLIGCWYCRRRNGYRALMDKSLHVGTQCALTRRCPQEGFDHRDSKVSLQEKNCEPVVPNAPPAYEKLSAEQSPPPYSP

Target ORF information:

RefSeq Version	NM_005511.1
Organism	Homo sapiens(human)
Definition	Homo sapiens melan-A (MLANA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005511.1

ORF Insert Sequence:

ATGCCAAGAG AAGATGCTCA CTTCATCTAT GGTTACCCCA AGAAGGGGCA CGGCCACTCT 
TACACCACGG CTGAAGAGGC CGCTGGGATC GGCATCCTGA CAGTGATCCT GGGAGTCTTA 
CTGCTCATCG GCTGTTGGTA TTGTAGAAGA CGAAATGGAT ACAGAGCCTT GATGGATAAA 
AGTCTTCATG TTGGCACTCA ATGTGCCTTA ACAAGAAGAT GCCCACAAGA AGGGTTTGAT 
CATCGGGACA GCAAAGTGTC TCTTCAAGAG AAAAACTGTG AACCTGTGGT TCCCAATGCT 
CCACCTGCTT ATGAGAAACT CTCTGCAGAA CAGTCACCAC CACCTTATTC ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Detection of Occult Invasion in Melanoma In Situ.
JAMA dermatology152(11)1201-1208(2016 Nov)
Bax MJ,Johnson TM,Harms PW,Schwartz JL,Zhao L,Fullen DR,Chan MP

Automated quantification of proliferation with automated hot-spot selection in phosphohistone H3/MART1 dual-stained stage I/II melanoma.
Diagnostic pathology1135(2016 Apr)
Nielsen PS,Riber-Hansen R,Schmidt H,Steiniche T

MelanA-negative spindle-cell associated melanoma, a distinct inflammatory phenotype correlated with dense infiltration of CD163 macrophages and loss of E-cadherin.
Melanoma research25(2)113-8(2015 Apr)
B?nnelykke-Behrndtz ML,Steiniche T,Damsgaard TE,Georgsen JB,Danielsen A,Bastholt L,M?ller HJ,N?rgaard PH,Schmidt H