GSPT2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GSPT2
Entrez Gene ID	23708
Full Name	G1 to S phase transition 2
Synonyms	ERF3B,GST2
General protein information	Preferred Names G1 to S phase transition 2 Names eukaryotic peptide chain release factor GTP-binding subunit ERF3B eukaryotic peptide chain release factor subunit 3b
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp11.22
Summary	This gene encodes a GTPase that belongs to the GTP-binding elongation factor family. The encoded protein is a polypeptide release factor that complexes with eukaryotic peptide chain release factor 1 to mediate translation termination. This protein may also be involved in mRNA stability.[provided by RefSeq, Mar 2010].
Disorder MIM:	300418

mRNA and Protein(s)

mRNA	Protein	Name
NM_018094.4	NP_060564.2	eukaryotic peptide chain release factor GTP-binding subunit ERF3B
NM_018094.5	NP_060564.2	eukaryotic peptide chain release factor GTP-binding subunit ERF3B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gspt2	NP_032205.2
Rattus norvegicus (Norway rat)	Gspt2	NP_001102789.1
Homo sapiens (human)	GSPT2	NP_060564.2
	GSPT2	XP_001100703.1
Caenorhabditis elegans (roundworm)	erfa-3	NP_001256292.1
Canis lupus familiaris (dog)	LOC480921	XP_005641416.1
Bos taurus (cattle)	GSPT2	NP_001192507.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Xp11.22 deletions encompassing CENPVL1, CENPVL2, MAGED1 and GSPT2 as a cause of syndromic X-linked intellectual disability.
Grau C, Starkovich M, Azamian MS, Xia F, Cheung SW, Evans P, Henderson A, Lalani SR, Scott DA
PloS one12(4)e0175962(2017)

The processed isoform of the translation termination factor eRF3 localizes to the nucleus to interact with the ARF tumor suppressor.
Hashimoto Y, Kumagai N, Hosoda N, Hoshino S
Biochemical and biophysical research communications445(3)639-44(2014 Mar)

eRF3b, a biomarker for hepatocellular carcinoma, influences cell cycle and phosphoralation status of 4E-BP1.
Li M, Wang J, Yang L, Gao P, Tian QB, Liu DW
PloS one9(1)e86371(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss of GSPT2 and/or MAGED1 function may contribute to the intellectual disability.
Title: Xp11.22 deletions encompassing CENPVL1, CENPVL2, MAGED1 and GSPT2 as a cause of syndromic X-linked intellectual disability.

The proteolytic cleavage of eRF3a and eRF3b into p-eRF3 leads to release an amino-terminal fragment containing nuclear export signal to allow the relocalization of eRF3 into the nucleus to interact with the p14ARF.
Title: The processed isoform of the translation termination factor eRF3 localizes to the nucleus to interact with the ARF tumor suppressor.

The tumor marker eRF3B can change the cell cycle and influence the phosphorylation status of 4E-BP1.
Title: eRF3b, a biomarker for hepatocellular carcinoma, influences cell cycle and phosphoralation status of 4E-BP1.

The survivin and eRF3 complex may function in spindle formation and segregation of chromosomes and cytokinesis.
Title: Polypeptide chain release factor eRF3 is a novel molecular partner of survivin.

eRF1's M domain contacts eRF3's GTPase domain
Title: Structural insights into eRF3 and stop codon recognition by eRF1.

The following GSPT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GSPT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23979	NM_018094.5 Latest version!	Homo sapiens G1 to S phase transition 2 (GSPT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu23979	NM_018094.4	Homo sapiens G1 to S phase transition 2 (GSPT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GSPT2 ( NM_018094.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060564.2 Homo sapiens G1 to S phase transition 2 (GSPT2), mRNA.

Price & Availability↑

CloneID	OHu23979
Clone ID Related Accession (Same CDS sequence)	NM_018094.4 , NM_018094.5
Accession Version	NM_018094.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	eukaryotic peptide chain release factor GTP-binding subunit ERF3B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB173006.1, AJ251548.1, AK001303.1 and BF514488.1. This sequence is a reference standard in the RefSeqGene project. On Mar 23, 2010 this sequence version replaced NM_018094.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGGATTCGG GCAGCAGCAG CAGCGACTCG GCGCCCGATT GCTGGGACCA GGTGGACATG 
GAATCCCCGG GGTCGGCCCC GAGCGGGGAT GGAGTCTCCT CTGCGGTGGC CGAGGCCCAG 
CGCGAGCCCC TCAGCTCGGC TTTCAGCCGT AAGCTCAACG TCAACGCCAA GCCCTTCGTG 
CCTAACGTAC ACGCCGCGGA GTTCGTGCCG TCCTTCCTGC GGGGCCCGAC TCAGCCGCCC 
ACCCTCCCGG CCGGCTCCGG CAGCAACGAT GAAACCTGCA CCGGCGCGGG ATACCCTCAA 
GGTAAAAGGA TGGGACGGGG GGCACCTGTG GAACCTTCCC GAGAGGAACC GTTAGTGTCG 
CTTGAAGGTT CCAATTCAGC CGTTACCATG GAACTTTCAG AACCTGTTGT AGAAAATGGA 
GAGGTGGAAA TGGCCCTAGA AGAATCATGG GAGCACAGTA AAGAAGTAAG TGAAGCCGAG 
CCTGGGGGTG GTTCCTCGGG AGATTCAGGG CCCCCAGAAG AAAGTGGCCA GGAAATGATG 
GAGGAAAAAG AGGAAATAAG AAAATCCAAA TCTGTGATCG TACCCTCAGG TGCACCTAAG 
AAAGAACACG TAAATGTAGT ATTCATTGGC CATGTAGACG CTGGCAAGTC AACCATCGGA 
GGACAGATAA TGTTTTTGAC TGGAATGGTT GACAAAAGAA CACTGGAGAA ATATGAAAGA 
GAAGCTAAGG AAAAAAACAG AGAAACCTGG TATTTGTCCT GGGCCTTAGA TACAAATCAG 
GAGGAACGAG ACAAGGGTAA AACAGTCGAA GTGGGTCGTG CCTATTTTGA AACAGAAAGG 
AAACATTTCA CAATTTTAGA TGCCCCTGGC CACAAGAGTT TTGTCCCAAA TATGATTGGT 
GGTGCTTCTC AAGCTGATTT GGCTGTGCTG GTCATCTCTG CCAGGAAAGG AGAGTTTGAA 
ACTGGATTTG AAAAAGGTGG ACAGACAAGA GAACATGCGA TGTTGGCAAA AACGGCAGGG 
GTAAAACATT TAATAGTGCT TATTAATAAG ATGGATGATC CCACAGTAAA TTGGAGCATC 
GAGAGATATG AAGAATGTAA AGAAAAACTG GTGCCCTTTT TGAAAAAAGT AGGCTTCAGT 
CCAAAAAAGG ACATTCACTT TATGCCCTGC TCAGGACTGA CCGGAGCAAA TATTAAAGAG 
CAGTCAGATT TCTGCCCTTG GTACACTGGA TTACCATTTA TTCCGTATTT GGATAACTTG 
CCAAACTTCA ACAGATCAAT TGATGGACCA ATAAGACTGC CAATTGTGGA TAAGTACAAA 
GATATGGGCA CTGTGGTCCT GGGAAAGCTG GAATCCGGGT CCATTTTTAA AGGCCAGCAG 
CTCGTGATGA TGCCAAACAA GCACAATGTA GAAGTTCTTG GAATACTTTC TGATGATACT 
GAAACTGATT TTGTAGCCCC AGGTGAAAAC CTCAAAATCA GACTGAAGGG AATTGAAGAA 
GAAGAGATTC TTCCAGGATT CATACTTTGT GATCCTAGTA ACCTCTGCCA TTCTGGACGC 
ACGTTTGATG TTCAGATAGT GATTATTGAG CACAAATCCA TCATCTGCCC AGGTTATAAT 
GCGGTGCTGC ACATTCATAC TTGTATTGAG GAAGTTGAGA TAACAGCGTT AATCTCCTTG 
GTAGACAAAA AATCAGGAGA AAAAAGTAAG ACACGACCCC GCTTCGTGAA ACAAGATCAA 
GTATGCATTG CTCGTTTAAG GACAGCAGGA ACCATCTGCC TCGAGACGTT CAAAGATTTT 
CCTCAGATGG GTCGTTTTAC TTTAAGAGAT GAGGGTAAGA CCATTGCAAT TGGAAAAGTT 
CTGAAATTGG TCCCAGAGAA GGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060564.2
CDS	243..2129
Translation	MDSGSSSSDSAPDCWDQVDMESPGSAPSGDGVSSAVAEAQREPLSSAFSRKLNVNAKPFVPNVHAAEFVPSFLRGPTQPPTLPAGSGSNDETCTGAGYPQGKRMGRGAPVEPSREEPLVSLEGSNSAVTMELSEPVVENGEVEMALEESWEHSKEVSEAEPGGGSSGDSGPPEESGQEMMEEKEEIRKSKSVIVPSGAPKKEHVNVVFIGHVDAGKSTIGGQIMFLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETERKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSIERYEECKEKLVPFLKKVGFSPKKDIHFMPCSGLTGANIKEQSDFCPWYTGLPFIPYLDNLPNFNRSIDGPIRLPIVDKYKDMGTVVLGKLESGSIFKGQQLVMMPNKHNVEVLGILSDDTETDFVAPGENLKIRLKGIEEEEILPGFILCDPSNLCHSGRTFDVQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALISLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD

Target ORF information:

RefSeq Version	NM_018094.4
Organism	Homo sapiens(human)
Definition	Homo sapiens G1 to S phase transition 2 (GSPT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018094.4

ORF Insert Sequence:

ATGGATTCGG GCAGCAGCAG CAGCGACTCG GCGCCCGATT GCTGGGACCA GGTGGACATG 
GAATCCCCGG GGTCGGCCCC GAGCGGGGAT GGAGTCTCCT CTGCGGTGGC CGAGGCCCAG 
CGCGAGCCCC TCAGCTCGGC TTTCAGCCGT AAGCTCAACG TCAACGCCAA GCCCTTCGTG 
CCTAACGTAC ACGCCGCGGA GTTCGTGCCG TCCTTCCTGC GGGGCCCGAC TCAGCCGCCC 
ACCCTCCCGG CCGGCTCCGG CAGCAACGAT GAAACCTGCA CCGGCGCGGG ATACCCTCAA 
GGTAAAAGGA TGGGACGGGG GGCACCTGTG GAACCTTCCC GAGAGGAACC GTTAGTGTCG 
CTTGAAGGTT CCAATTCAGC CGTTACCATG GAACTTTCAG AACCTGTTGT AGAAAATGGA 
GAGGTGGAAA TGGCCCTAGA AGAATCATGG GAGCACAGTA AAGAAGTAAG TGAAGCCGAG 
CCTGGGGGTG GTTCCTCGGG AGATTCAGGG CCCCCAGAAG AAAGTGGCCA GGAAATGATG 
GAGGAAAAAG AGGAAATAAG AAAATCCAAA TCTGTGATCG TACCCTCAGG TGCACCTAAG 
AAAGAACACG TAAATGTAGT ATTCATTGGC CATGTAGACG CTGGCAAGTC AACCATCGGA 
GGACAGATAA TGTTTTTGAC TGGAATGGTT GACAAAAGAA CACTGGAGAA ATATGAAAGA 
GAAGCTAAGG AAAAAAACAG AGAAACCTGG TATTTGTCCT GGGCCTTAGA TACAAATCAG 
GAGGAACGAG ACAAGGGTAA AACAGTCGAA GTGGGTCGTG CCTATTTTGA AACAGAAAGG 
AAACATTTCA CAATTTTAGA TGCCCCTGGC CACAAGAGTT TTGTCCCAAA TATGATTGGT 
GGTGCTTCTC AAGCTGATTT GGCTGTGCTG GTCATCTCTG CCAGGAAAGG AGAGTTTGAA 
ACTGGATTTG AAAAAGGTGG ACAGACAAGA GAACATGCGA TGTTGGCAAA AACGGCAGGG 
GTAAAACATT TAATAGTGCT TATTAATAAG ATGGATGATC CCACAGTAAA TTGGAGCATC 
GAGAGATATG AAGAATGTAA AGAAAAACTG GTGCCCTTTT TGAAAAAAGT AGGCTTCAGT 
CCAAAAAAGG ACATTCACTT TATGCCCTGC TCAGGACTGA CCGGAGCAAA TATTAAAGAG 
CAGTCAGATT TCTGCCCTTG GTACACTGGA TTACCATTTA TTCCGTATTT GGATAACTTG 
CCAAACTTCA ACAGATCAAT TGATGGACCA ATAAGACTGC CAATTGTGGA TAAGTACAAA 
GATATGGGCA CTGTGGTCCT GGGAAAGCTG GAATCCGGGT CCATTTTTAA AGGCCAGCAG 
CTCGTGATGA TGCCAAACAA GCACAATGTA GAAGTTCTTG GAATACTTTC TGATGATACT 
GAAACTGATT TTGTAGCCCC AGGTGAAAAC CTCAAAATCA GACTGAAGGG AATTGAAGAA 
GAAGAGATTC TTCCAGGATT CATACTTTGT GATCCTAGTA ACCTCTGCCA TTCTGGACGC 
ACGTTTGATG TTCAGATAGT GATTATTGAG CACAAATCCA TCATCTGCCC AGGTTATAAT 
GCGGTGCTGC ACATTCATAC TTGTATTGAG GAAGTTGAGA TAACAGCGTT AATCTCCTTG 
GTAGACAAAA AATCAGGAGA AAAAAGTAAG ACACGACCCC GCTTCGTGAA ACAAGATCAA 
GTATGCATTG CTCGTTTAAG GACAGCAGGA ACCATCTGCC TCGAGACGTT CAAAGATTTT 
CCTCAGATGG GTCGTTTTAC TTTAAGAGAT GAGGGTAAGA CCATTGCAAT TGGAAAAGTT 
CTGAAATTGG TCCCAGAGAA GGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GSPT2 ( NM_018094.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060564.2 Homo sapiens G1 to S phase transition 2 (GSPT2), mRNA.

Price & Availability↑

CloneID	OHu23979
Clone ID Related Accession (Same CDS sequence)	NM_018094.4 , NM_018094.5
Accession Version	NM_018094.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	eukaryotic peptide chain release factor GTP-binding subunit ERF3B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB173006.1, AJ251548.1, AK001303.1 and BF514488.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_018094.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: SRR1803615.204101.1, SRR1660805.209605.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000340438.6/ ENSP00000341247.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGATTCGG GCAGCAGCAG CAGCGACTCG GCGCCCGATT GCTGGGACCA GGTGGACATG 
GAATCCCCGG GGTCGGCCCC GAGCGGGGAT GGAGTCTCCT CTGCGGTGGC CGAGGCCCAG 
CGCGAGCCCC TCAGCTCGGC TTTCAGCCGT AAGCTCAACG TCAACGCCAA GCCCTTCGTG 
CCTAACGTAC ACGCCGCGGA GTTCGTGCCG TCCTTCCTGC GGGGCCCGAC TCAGCCGCCC 
ACCCTCCCGG CCGGCTCCGG CAGCAACGAT GAAACCTGCA CCGGCGCGGG ATACCCTCAA 
GGTAAAAGGA TGGGACGGGG GGCACCTGTG GAACCTTCCC GAGAGGAACC GTTAGTGTCG 
CTTGAAGGTT CCAATTCAGC CGTTACCATG GAACTTTCAG AACCTGTTGT AGAAAATGGA 
GAGGTGGAAA TGGCCCTAGA AGAATCATGG GAGCACAGTA AAGAAGTAAG TGAAGCCGAG 
CCTGGGGGTG GTTCCTCGGG AGATTCAGGG CCCCCAGAAG AAAGTGGCCA GGAAATGATG 
GAGGAAAAAG AGGAAATAAG AAAATCCAAA TCTGTGATCG TACCCTCAGG TGCACCTAAG 
AAAGAACACG TAAATGTAGT ATTCATTGGC CATGTAGACG CTGGCAAGTC AACCATCGGA 
GGACAGATAA TGTTTTTGAC TGGAATGGTT GACAAAAGAA CACTGGAGAA ATATGAAAGA 
GAAGCTAAGG AAAAAAACAG AGAAACCTGG TATTTGTCCT GGGCCTTAGA TACAAATCAG 
GAGGAACGAG ACAAGGGTAA AACAGTCGAA GTGGGTCGTG CCTATTTTGA AACAGAAAGG 
AAACATTTCA CAATTTTAGA TGCCCCTGGC CACAAGAGTT TTGTCCCAAA TATGATTGGT 
GGTGCTTCTC AAGCTGATTT GGCTGTGCTG GTCATCTCTG CCAGGAAAGG AGAGTTTGAA 
ACTGGATTTG AAAAAGGTGG ACAGACAAGA GAACATGCGA TGTTGGCAAA AACGGCAGGG 
GTAAAACATT TAATAGTGCT TATTAATAAG ATGGATGATC CCACAGTAAA TTGGAGCATC 
GAGAGATATG AAGAATGTAA AGAAAAACTG GTGCCCTTTT TGAAAAAAGT AGGCTTCAGT 
CCAAAAAAGG ACATTCACTT TATGCCCTGC TCAGGACTGA CCGGAGCAAA TATTAAAGAG 
CAGTCAGATT TCTGCCCTTG GTACACTGGA TTACCATTTA TTCCGTATTT GGATAACTTG 
CCAAACTTCA ACAGATCAAT TGATGGACCA ATAAGACTGC CAATTGTGGA TAAGTACAAA 
GATATGGGCA CTGTGGTCCT GGGAAAGCTG GAATCCGGGT CCATTTTTAA AGGCCAGCAG 
CTCGTGATGA TGCCAAACAA GCACAATGTA GAAGTTCTTG GAATACTTTC TGATGATACT 
GAAACTGATT TTGTAGCCCC AGGTGAAAAC CTCAAAATCA GACTGAAGGG AATTGAAGAA 
GAAGAGATTC TTCCAGGATT CATACTTTGT GATCCTAGTA ACCTCTGCCA TTCTGGACGC 
ACGTTTGATG TTCAGATAGT GATTATTGAG CACAAATCCA TCATCTGCCC AGGTTATAAT 
GCGGTGCTGC ACATTCATAC TTGTATTGAG GAAGTTGAGA TAACAGCGTT AATCTCCTTG 
GTAGACAAAA AATCAGGAGA AAAAAGTAAG ACACGACCCC GCTTCGTGAA ACAAGATCAA 
GTATGCATTG CTCGTTTAAG GACAGCAGGA ACCATCTGCC TCGAGACGTT CAAAGATTTT 
CCTCAGATGG GTCGTTTTAC TTTAAGAGAT GAGGGTAAGA CCATTGCAAT TGGAAAAGTT 
CTGAAATTGG TCCCAGAGAA GGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060564.2
CDS	186..2072
Translation	MDSGSSSSDSAPDCWDQVDMESPGSAPSGDGVSSAVAEAQREPLSSAFSRKLNVNAKPFVPNVHAAEFVPSFLRGPTQPPTLPAGSGSNDETCTGAGYPQGKRMGRGAPVEPSREEPLVSLEGSNSAVTMELSEPVVENGEVEMALEESWEHSKEVSEAEPGGGSSGDSGPPEESGQEMMEEKEEIRKSKSVIVPSGAPKKEHVNVVFIGHVDAGKSTIGGQIMFLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETERKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSIERYEECKEKLVPFLKKVGFSPKKDIHFMPCSGLTGANIKEQSDFCPWYTGLPFIPYLDNLPNFNRSIDGPIRLPIVDKYKDMGTVVLGKLESGSIFKGQQLVMMPNKHNVEVLGILSDDTETDFVAPGENLKIRLKGIEEEEILPGFILCDPSNLCHSGRTFDVQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALISLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD

Target ORF information:

RefSeq Version	NM_018094.5
Organism	Homo sapiens(human)
Definition	Homo sapiens G1 to S phase transition 2 (GSPT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018094.5

ORF Insert Sequence:

ATGGATTCGG GCAGCAGCAG CAGCGACTCG GCGCCCGATT GCTGGGACCA GGTGGACATG 
GAATCCCCGG GGTCGGCCCC GAGCGGGGAT GGAGTCTCCT CTGCGGTGGC CGAGGCCCAG 
CGCGAGCCCC TCAGCTCGGC TTTCAGCCGT AAGCTCAACG TCAACGCCAA GCCCTTCGTG 
CCTAACGTAC ACGCCGCGGA GTTCGTGCCG TCCTTCCTGC GGGGCCCGAC TCAGCCGCCC 
ACCCTCCCGG CCGGCTCCGG CAGCAACGAT GAAACCTGCA CCGGCGCGGG ATACCCTCAA 
GGTAAAAGGA TGGGACGGGG GGCACCTGTG GAACCTTCCC GAGAGGAACC GTTAGTGTCG 
CTTGAAGGTT CCAATTCAGC CGTTACCATG GAACTTTCAG AACCTGTTGT AGAAAATGGA 
GAGGTGGAAA TGGCCCTAGA AGAATCATGG GAGCACAGTA AAGAAGTAAG TGAAGCCGAG 
CCTGGGGGTG GTTCCTCGGG AGATTCAGGG CCCCCAGAAG AAAGTGGCCA GGAAATGATG 
GAGGAAAAAG AGGAAATAAG AAAATCCAAA TCTGTGATCG TACCCTCAGG TGCACCTAAG 
AAAGAACACG TAAATGTAGT ATTCATTGGC CATGTAGACG CTGGCAAGTC AACCATCGGA 
GGACAGATAA TGTTTTTGAC TGGAATGGTT GACAAAAGAA CACTGGAGAA ATATGAAAGA 
GAAGCTAAGG AAAAAAACAG AGAAACCTGG TATTTGTCCT GGGCCTTAGA TACAAATCAG 
GAGGAACGAG ACAAGGGTAA AACAGTCGAA GTGGGTCGTG CCTATTTTGA AACAGAAAGG 
AAACATTTCA CAATTTTAGA TGCCCCTGGC CACAAGAGTT TTGTCCCAAA TATGATTGGT 
GGTGCTTCTC AAGCTGATTT GGCTGTGCTG GTCATCTCTG CCAGGAAAGG AGAGTTTGAA 
ACTGGATTTG AAAAAGGTGG ACAGACAAGA GAACATGCGA TGTTGGCAAA AACGGCAGGG 
GTAAAACATT TAATAGTGCT TATTAATAAG ATGGATGATC CCACAGTAAA TTGGAGCATC 
GAGAGATATG AAGAATGTAA AGAAAAACTG GTGCCCTTTT TGAAAAAAGT AGGCTTCAGT 
CCAAAAAAGG ACATTCACTT TATGCCCTGC TCAGGACTGA CCGGAGCAAA TATTAAAGAG 
CAGTCAGATT TCTGCCCTTG GTACACTGGA TTACCATTTA TTCCGTATTT GGATAACTTG 
CCAAACTTCA ACAGATCAAT TGATGGACCA ATAAGACTGC CAATTGTGGA TAAGTACAAA 
GATATGGGCA CTGTGGTCCT GGGAAAGCTG GAATCCGGGT CCATTTTTAA AGGCCAGCAG 
CTCGTGATGA TGCCAAACAA GCACAATGTA GAAGTTCTTG GAATACTTTC TGATGATACT 
GAAACTGATT TTGTAGCCCC AGGTGAAAAC CTCAAAATCA GACTGAAGGG AATTGAAGAA 
GAAGAGATTC TTCCAGGATT CATACTTTGT GATCCTAGTA ACCTCTGCCA TTCTGGACGC 
ACGTTTGATG TTCAGATAGT GATTATTGAG CACAAATCCA TCATCTGCCC AGGTTATAAT 
GCGGTGCTGC ACATTCATAC TTGTATTGAG GAAGTTGAGA TAACAGCGTT AATCTCCTTG 
GTAGACAAAA AATCAGGAGA AAAAAGTAAG ACACGACCCC GCTTCGTGAA ACAAGATCAA 
GTATGCATTG CTCGTTTAAG GACAGCAGGA ACCATCTGCC TCGAGACGTT CAAAGATTTT 
CCTCAGATGG GTCGTTTTAC TTTAAGAGAT GAGGGTAAGA CCATTGCAAT TGGAAAAGTT 
CTGAAATTGG TCCCAGAGAA GGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Xp11.22 deletions encompassing CENPVL1, CENPVL2, MAGED1 and GSPT2 as a cause of syndromic X-linked intellectual disability.
PloS one12(4)e0175962(2017)
Grau C,Starkovich M,Azamian MS,Xia F,Cheung SW,Evans P,Henderson A,Lalani SR,Scott DA

The processed isoform of the translation termination factor eRF3 localizes to the nucleus to interact with the ARF tumor suppressor.
Biochemical and biophysical research communications445(3)639-44(2014 Mar)
Hashimoto Y,Kumagai N,Hosoda N,Hoshino S

eRF3b, a biomarker for hepatocellular carcinoma, influences cell cycle and phosphoralation status of 4E-BP1.
PloS one9(1)e86371(2014)
Li M,Wang J,Yang L,Gao P,Tian QB,Liu DW